Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1175410 | AAA61914.1 | 98.540 | 274 | 3 | 1 | 1 | 274 | 1 | 273 | 0.0 | 544 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1175410 | sp|Q01806|LEC1_MEDTR | 95.000 | 260 | 10 | 2 | 1 | 258 | 3 | 261 | 2.15e-180 | 500 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0216340 | Msa1175410 | 0.801253 | 9.823951e-49 | -8.615850e-47 |
| Msa1119440 | Msa1175410 | 0.808643 | 2.799852e-50 | -8.615850e-47 |
| Msa1175410 | Msa1235430 | 0.831660 | 1.471548e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1175410 | MtrunA17_Chr8g0345451 | 95.000 | 260 | 10 | 2 | 1 | 258 | 3 | 261 | 0.0 | 500 |
| Msa1175410 | MtrunA17_Chr3g0104481 | 59.574 | 282 | 65 | 6 | 1 | 274 | 1 | 241 | 1.02e-99 | 291 |
| Msa1175410 | MtrunA17_Chr4g0011021 | 60.236 | 254 | 85 | 6 | 23 | 274 | 33 | 272 | 1.07e-99 | 293 |
| Msa1175410 | MtrunA17_Chr8g0367061 | 37.743 | 257 | 145 | 7 | 10 | 256 | 10 | 261 | 8.65e-49 | 162 |
| Msa1175410 | MtrunA17_Chr5g0411741 | 39.583 | 240 | 129 | 5 | 32 | 256 | 40 | 278 | 5.12e-48 | 160 |
| Msa1175410 | MtrunA17_Chr8g0367051 | 36.398 | 261 | 147 | 7 | 10 | 256 | 10 | 265 | 5.78e-47 | 157 |
| Msa1175410 | MtrunA17_Chr5g0411771 | 38.800 | 250 | 133 | 8 | 24 | 256 | 41 | 287 | 2.59e-45 | 154 |
| Msa1175410 | MtrunA17_Chr5g0411751 | 39.004 | 241 | 130 | 6 | 32 | 256 | 40 | 279 | 1.37e-44 | 152 |
| Msa1175410 | MtrunA17_Chr5g0411761 | 38.333 | 240 | 132 | 7 | 32 | 256 | 48 | 286 | 3.37e-43 | 148 |
| Msa1175410 | MtrunA17_Chr8g0367071 | 39.313 | 262 | 138 | 10 | 9 | 256 | 19 | 273 | 1.81e-42 | 154 |
| Msa1175410 | MtrunA17_Chr3g0096161 | 38.636 | 264 | 150 | 8 | 6 | 263 | 11 | 268 | 6.76e-41 | 142 |
| Msa1175410 | MtrunA17_Chr5g0411721 | 38.306 | 248 | 136 | 9 | 23 | 256 | 38 | 282 | 6.68e-40 | 140 |
| Msa1175410 | MtrunA17_Chr3g0100011 | 42.553 | 235 | 123 | 8 | 6 | 236 | 34 | 260 | 1.76e-39 | 141 |
| Msa1175410 | MtrunA17_Chr5g0411731 | 37.603 | 242 | 133 | 8 | 32 | 256 | 35 | 275 | 2.52e-39 | 138 |
| Msa1175410 | MtrunA17_Chr3g0100021 | 41.702 | 235 | 124 | 7 | 25 | 253 | 14 | 241 | 4.94e-39 | 137 |
| Msa1175410 | MtrunA17_Chr3g0087611 | 39.526 | 253 | 132 | 10 | 13 | 252 | 18 | 262 | 4.53e-38 | 135 |
| Msa1175410 | MtrunA17_Chr8g0367031 | 40.756 | 238 | 120 | 12 | 32 | 254 | 26 | 257 | 1.00e-37 | 133 |
| Msa1175410 | MtrunA17_Chr3g0095861 | 36.996 | 273 | 157 | 9 | 6 | 269 | 10 | 276 | 8.80e-36 | 129 |
| Msa1175410 | MtrunA17_Chr3g0095851 | 36.996 | 273 | 157 | 9 | 6 | 269 | 11 | 277 | 9.39e-36 | 129 |
| Msa1175410 | MtrunA17_Chr4g0049601 | 34.082 | 267 | 163 | 10 | 15 | 271 | 19 | 282 | 1.10e-34 | 132 |
| Msa1175410 | MtrunA17_Chr4g0049611 | 34.483 | 261 | 150 | 9 | 8 | 256 | 13 | 264 | 1.36e-34 | 131 |
| Msa1175410 | MtrunA17_Chr1g0197771 | 38.168 | 262 | 148 | 11 | 2 | 256 | 6 | 260 | 5.59e-34 | 124 |
| Msa1175410 | MtrunA17_Chr8g0366941 | 38.627 | 233 | 132 | 8 | 33 | 256 | 33 | 263 | 1.35e-33 | 124 |
| Msa1175410 | MtrunA17_Chr3g0100031 | 37.008 | 254 | 149 | 8 | 9 | 257 | 8 | 255 | 2.29e-33 | 122 |
| Msa1175410 | MtrunA17_Chr8g0367011 | 33.818 | 275 | 154 | 9 | 7 | 256 | 24 | 295 | 7.80e-33 | 122 |
| Msa1175410 | MtrunA17_Chr1g0197791 | 36.508 | 252 | 125 | 10 | 13 | 252 | 18 | 246 | 9.49e-33 | 120 |
| Msa1175410 | MtrunA17_Chr8g0367041 | 36.842 | 247 | 132 | 13 | 32 | 261 | 26 | 265 | 7.44e-32 | 118 |
| Msa1175410 | MtrunA17_Chr8g0366991 | 32.727 | 275 | 170 | 7 | 1 | 266 | 101 | 369 | 1.16e-29 | 117 |
| Msa1175410 | MtrunA17_Chr4g0049581 | 30.182 | 275 | 165 | 9 | 2 | 258 | 21 | 286 | 1.46e-29 | 117 |
| Msa1175410 | MtrunA17_Chr7g0274651 | 32.422 | 256 | 150 | 10 | 19 | 258 | 25 | 273 | 4.15e-26 | 107 |
| Msa1175410 | MtrunA17_Chr8g0367021 | 33.482 | 224 | 131 | 9 | 45 | 256 | 41 | 258 | 1.09e-24 | 99.4 |
| Msa1175410 | MtrunA17_Chr8g0366981 | 28.464 | 267 | 148 | 5 | 1 | 264 | 1 | 227 | 2.54e-24 | 102 |
| Msa1175410 | MtrunA17_Chr1g0197761 | 32.937 | 252 | 146 | 10 | 16 | 256 | 21 | 260 | 4.33e-24 | 98.2 |
| Msa1175410 | MtrunA17_Chr4g0049551 | 35.638 | 188 | 110 | 6 | 68 | 245 | 73 | 259 | 5.11e-24 | 101 |
| Msa1175410 | MtrunA17_Chr7g0274661 | 34.498 | 229 | 128 | 9 | 44 | 256 | 57 | 279 | 6.50e-24 | 100 |
| Msa1175410 | MtrunA17_Chr1g0197801 | 32.197 | 264 | 130 | 11 | 5 | 257 | 9 | 234 | 3.00e-23 | 95.1 |
| Msa1175410 | MtrunA17_Chr1g0170201 | 33.810 | 210 | 119 | 8 | 56 | 254 | 79 | 279 | 1.64e-22 | 97.1 |
| Msa1175410 | MtrunA17_Chr1g0197751 | 39.378 | 193 | 101 | 9 | 68 | 256 | 3 | 183 | 7.51e-22 | 90.5 |
| Msa1175410 | MtrunA17_Chr7g0239321 | 31.621 | 253 | 153 | 8 | 17 | 252 | 6 | 255 | 4.24e-21 | 92.8 |
| Msa1175410 | MtrunA17_Chr7g0239381 | 31.439 | 264 | 156 | 8 | 5 | 252 | 6 | 260 | 2.44e-19 | 87.4 |
| Msa1175410 | MtrunA17_Chr7g0239331 | 31.349 | 252 | 153 | 7 | 18 | 252 | 7 | 255 | 3.40e-19 | 87.0 |
| Msa1175410 | MtrunA17_Chr4g0049561 | 32.308 | 260 | 144 | 12 | 16 | 252 | 12 | 262 | 4.14e-19 | 87.0 |
| Msa1175410 | MtrunA17_Chr8g0366961 | 32.895 | 152 | 97 | 3 | 33 | 180 | 46 | 196 | 4.33e-19 | 83.2 |
| Msa1175410 | MtrunA17_Chr7g0239221 | 31.250 | 256 | 155 | 6 | 17 | 252 | 6 | 260 | 2.42e-18 | 84.7 |
| Msa1175410 | MtrunA17_Chr4g0027601 | 28.571 | 266 | 126 | 10 | 9 | 257 | 12 | 230 | 6.63e-18 | 80.9 |
| Msa1175410 | MtrunA17_Chr5g0414561 | 30.651 | 261 | 153 | 10 | 15 | 252 | 8 | 263 | 1.73e-17 | 82.0 |
| Msa1175410 | MtrunA17_Chr1g0172951 | 30.888 | 259 | 155 | 7 | 15 | 252 | 3 | 258 | 2.15e-17 | 81.6 |
| Msa1175410 | MtrunA17_Chr7g0239261 | 31.102 | 254 | 154 | 8 | 17 | 252 | 6 | 256 | 2.35e-17 | 81.6 |
| Msa1175410 | MtrunA17_Chr7g0239191 | 31.518 | 257 | 151 | 8 | 17 | 252 | 37 | 289 | 7.86e-17 | 80.1 |
| Msa1175410 | MtrunA17_Chr5g0407721 | 30.233 | 215 | 124 | 7 | 67 | 263 | 85 | 291 | 1.04e-16 | 79.7 |
| Msa1175410 | MtrunA17_Chr5g0433941 | 31.771 | 192 | 115 | 5 | 70 | 254 | 68 | 250 | 3.17e-16 | 78.6 |
| Msa1175410 | MtrunA17_Chr7g0239371 | 28.185 | 259 | 165 | 6 | 9 | 252 | 4 | 256 | 1.49e-15 | 76.3 |
| Msa1175410 | MtrunA17_Chr7g0239211 | 28.906 | 256 | 159 | 7 | 17 | 252 | 6 | 258 | 1.95e-15 | 75.9 |
| Msa1175410 | MtrunA17_Chr5g0428851 | 29.457 | 258 | 148 | 12 | 23 | 254 | 28 | 277 | 3.01e-15 | 75.5 |
| Msa1175410 | MtrunA17_Chr3g0112111 | 30.769 | 260 | 157 | 8 | 15 | 252 | 9 | 267 | 1.76e-14 | 73.2 |
| Msa1175410 | MtrunA17_Chr7g0239361 | 27.444 | 266 | 175 | 5 | 4 | 252 | 6 | 270 | 6.81e-14 | 71.6 |
| Msa1175410 | MtrunA17_Chr2g0282271 | 30.370 | 270 | 153 | 11 | 5 | 252 | 6 | 262 | 1.48e-13 | 70.5 |
| Msa1175410 | MtrunA17_Chr7g0239311 | 29.688 | 256 | 157 | 7 | 17 | 252 | 6 | 258 | 2.52e-13 | 69.7 |
| Msa1175410 | MtrunA17_Chr7g0239171 | 31.225 | 253 | 154 | 7 | 17 | 252 | 6 | 255 | 9.29e-13 | 68.2 |
| Msa1175410 | MtrunA17_Chr7g0239281 | 26.718 | 262 | 156 | 6 | 6 | 252 | 4 | 244 | 1.10e-12 | 67.8 |
| Msa1175410 | MtrunA17_Chr7g0239181 | 29.694 | 229 | 140 | 5 | 44 | 252 | 32 | 259 | 7.93e-12 | 65.1 |
| Msa1175410 | MtrunA17_Chr1g0202201 | 26.016 | 246 | 151 | 9 | 30 | 254 | 14 | 249 | 1.47e-11 | 64.3 |
| Msa1175410 | MtrunA17_Chr5g0446651 | 30.769 | 221 | 132 | 6 | 44 | 254 | 38 | 247 | 2.18e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1175410 | AT5G65600.1 | 33.778 | 225 | 137 | 7 | 33 | 245 | 38 | 262 | 7.97e-25 | 103 |
| Msa1175410 | AT5G10530.1 | 33.462 | 260 | 155 | 10 | 12 | 259 | 4 | 257 | 1.22e-24 | 103 |
| Msa1175410 | AT4G02410.1 | 32.231 | 242 | 147 | 6 | 27 | 252 | 23 | 263 | 4.25e-23 | 99.0 |
| Msa1175410 | AT2G37710.1 | 30.667 | 225 | 139 | 6 | 44 | 252 | 38 | 261 | 6.56e-22 | 95.5 |
| Msa1175410 | AT4G02420.1 | 30.682 | 264 | 161 | 9 | 6 | 252 | 2 | 260 | 1.13e-21 | 94.7 |
| Msa1175410 | AT5G01540.1 | 31.200 | 250 | 144 | 9 | 31 | 254 | 31 | 278 | 6.68e-21 | 92.4 |
| Msa1175410 | AT3G55550.1 | 30.705 | 241 | 152 | 4 | 27 | 253 | 20 | 259 | 1.70e-20 | 91.3 |
| Msa1175410 | AT2G29250.1 | 31.179 | 263 | 146 | 9 | 17 | 255 | 13 | 264 | 1.76e-20 | 91.3 |
| Msa1175410 | AT4G29050.1 | 33.186 | 226 | 123 | 8 | 49 | 252 | 39 | 258 | 4.45e-20 | 90.1 |
| Msa1175410 | AT4G29050.2 | 33.186 | 226 | 123 | 8 | 49 | 252 | 53 | 272 | 4.49e-20 | 90.1 |
| Msa1175410 | AT3G53810.1 | 30.488 | 246 | 152 | 7 | 25 | 253 | 19 | 262 | 2.68e-19 | 87.8 |
| Msa1175410 | AT5G01550.1 | 29.181 | 281 | 160 | 11 | 9 | 254 | 14 | 290 | 9.52e-19 | 86.3 |
| Msa1175410 | AT5G01560.1 | 29.482 | 251 | 142 | 10 | 33 | 254 | 30 | 274 | 1.40e-17 | 82.8 |
| Msa1175410 | AT5G55830.1 | 29.740 | 269 | 152 | 12 | 7 | 254 | 14 | 266 | 4.37e-17 | 81.3 |
| Msa1175410 | AT3G15356.1 | 28.689 | 244 | 142 | 8 | 41 | 253 | 28 | 270 | 6.33e-17 | 79.0 |
| Msa1175410 | AT3G08870.2 | 29.963 | 267 | 155 | 11 | 18 | 254 | 37 | 301 | 7.65e-17 | 80.5 |
| Msa1175410 | AT3G08870.1 | 29.963 | 267 | 155 | 11 | 18 | 254 | 16 | 280 | 8.79e-17 | 80.5 |
| Msa1175410 | AT2G29220.1 | 30.216 | 278 | 149 | 12 | 8 | 255 | 2 | 264 | 1.15e-16 | 80.1 |
| Msa1175410 | AT5G03140.1 | 30.392 | 204 | 128 | 6 | 57 | 250 | 58 | 257 | 1.28e-16 | 80.1 |
| Msa1175410 | AT5G60280.1 | 28.405 | 257 | 159 | 9 | 13 | 252 | 8 | 256 | 7.40e-16 | 77.8 |
| Msa1175410 | AT5G60310.1 | 29.278 | 263 | 156 | 10 | 10 | 252 | 5 | 257 | 1.07e-15 | 77.0 |
| Msa1175410 | AT3G45430.2 | 31.679 | 262 | 137 | 13 | 27 | 261 | 22 | 268 | 1.83e-15 | 76.6 |
| Msa1175410 | AT3G45430.3 | 31.679 | 262 | 137 | 13 | 27 | 261 | 22 | 268 | 1.83e-15 | 76.6 |
| Msa1175410 | AT3G45430.1 | 31.679 | 262 | 137 | 13 | 27 | 261 | 22 | 268 | 1.83e-15 | 76.6 |
| Msa1175410 | AT3G16530.1 | 27.049 | 244 | 146 | 8 | 41 | 253 | 28 | 270 | 1.57e-14 | 72.4 |
| Msa1175410 | AT5G06740.2 | 31.000 | 200 | 121 | 9 | 65 | 254 | 78 | 270 | 4.29e-14 | 72.4 |
| Msa1175410 | AT5G06740.1 | 31.000 | 200 | 121 | 9 | 65 | 254 | 66 | 258 | 4.72e-14 | 72.4 |
| Msa1175410 | AT4G04960.2 | 31.780 | 236 | 138 | 9 | 33 | 250 | 23 | 253 | 4.77e-14 | 72.4 |
| Msa1175410 | AT4G04960.1 | 31.780 | 236 | 138 | 9 | 33 | 250 | 23 | 253 | 8.96e-14 | 71.6 |
| Msa1175410 | AT5G60300.1 | 29.918 | 244 | 139 | 12 | 31 | 252 | 25 | 258 | 1.01e-13 | 71.2 |
| Msa1175410 | AT5G60300.2 | 29.918 | 244 | 139 | 12 | 31 | 252 | 25 | 258 | 1.01e-13 | 71.2 |
| Msa1175410 | AT5G60300.3 | 29.918 | 244 | 139 | 12 | 31 | 252 | 25 | 258 | 1.05e-13 | 71.2 |
| Msa1175410 | AT1G70110.1 | 27.706 | 231 | 138 | 10 | 44 | 252 | 33 | 256 | 4.21e-13 | 69.3 |
| Msa1175410 | AT3G59700.1 | 25.641 | 234 | 155 | 7 | 35 | 250 | 17 | 249 | 1.16e-12 | 68.2 |
| Msa1175410 | AT2G43700.1 | 29.796 | 245 | 145 | 8 | 30 | 254 | 14 | 251 | 2.16e-12 | 67.4 |
| Msa1175410 | AT3G45330.1 | 28.112 | 249 | 161 | 7 | 27 | 259 | 24 | 270 | 2.38e-12 | 67.4 |
| Msa1175410 | AT5G42120.1 | 29.707 | 239 | 140 | 8 | 37 | 254 | 27 | 258 | 4.82e-12 | 66.2 |
| Msa1175410 | AT3G45410.1 | 26.966 | 267 | 166 | 12 | 3 | 252 | 2 | 256 | 1.59e-11 | 64.7 |
| Msa1175410 | AT3G53380.1 | 31.873 | 251 | 151 | 10 | 10 | 250 | 4 | 244 | 1.82e-11 | 64.7 |
| Msa1175410 | AT5G60320.1 | 27.799 | 259 | 159 | 12 | 17 | 252 | 9 | 262 | 1.93e-11 | 64.7 |
Find 51 sgRNAs with CRISPR-Local
Find 122 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGGAAGATTAGATTCTTT+TGG | 0.238719 | 8_1:-6724415 | None:intergenic |
| CAGAACATGATATTCGTTAT+TGG | 0.241480 | 8_1:+6725020 | Msa1175410:CDS |
| ATTCTGCTCCTATTCATATC+TGG | 0.244024 | 8_1:+6724513 | Msa1175410:CDS |
| CCAATATGTCTATTTATCTT+TGG | 0.248398 | 8_1:-6724767 | None:intergenic |
| CTTCATCTTGTTATTCAATA+AGG | 0.258451 | 8_1:+6724343 | Msa1175410:CDS |
| AAGCACTACACCATGGGTTT+TGG | 0.288100 | 8_1:+6724817 | Msa1175410:CDS |
| CTTCCCTGTTGAGGTAGTTA+TGG | 0.356339 | 8_1:-6724444 | None:intergenic |
| ATCGGTCGTGCAGGATTTCT+CGG | 0.366627 | 8_1:+6724665 | Msa1175410:CDS |
| TTGCTATCCCAGATATGAAT+AGG | 0.375848 | 8_1:-6724521 | None:intergenic |
| CATATCTGGGATAGCAAAAC+AGG | 0.382485 | 8_1:+6724527 | Msa1175410:CDS |
| CCAAAGATAAATAGACATAT+TGG | 0.396965 | 8_1:+6724767 | Msa1175410:CDS |
| TACCAATGTTGCAGATGGTT+TGG | 0.397603 | 8_1:+6724604 | Msa1175410:CDS |
| TCACTAAGTATATAACTATC+AGG | 0.404845 | 8_1:-6724923 | None:intergenic |
| TCTGCAACATTGGTACTATA+AGG | 0.412329 | 8_1:-6724596 | None:intergenic |
| GGTACTATAAGGAGCGGTTA+TGG | 0.427914 | 8_1:-6724585 | None:intergenic |
| TAACAAGATGAAGACTGTTA+AGG | 0.442595 | 8_1:-6724333 | None:intergenic |
| AAGGCAGTCAAGAACTCTAT+TGG | 0.450440 | 8_1:+6724479 | Msa1175410:CDS |
| TAGTGATGTTGTGCCTTTGA+AGG | 0.455186 | 8_1:+6724940 | Msa1175410:CDS |
| AATGCCATAACTACCTCAAC+AGG | 0.458397 | 8_1:+6724440 | Msa1175410:CDS |
| TTCTGCTCCTATTCATATCT+GGG | 0.459119 | 8_1:+6724514 | Msa1175410:CDS |
| GAGAGGCTAATGTTGTGATA+AGG | 0.465611 | 8_1:+6724849 | Msa1175410:CDS |
| GCTAAACTTTGTGATGGTAA+AGG | 0.469149 | 8_1:-6724387 | None:intergenic |
| GGCCAAACCATCTGCAACAT+TGG | 0.479659 | 8_1:-6724606 | None:intergenic |
| CCACAAAACATCGGTCGTGC+AGG | 0.494269 | 8_1:+6724656 | Msa1175410:CDS |
| AATAGAGTTCTTGACTGCCT+TGG | 0.497569 | 8_1:-6724477 | None:intergenic |
| ATATTGTTCCTGAGTGGGTA+AGG | 0.500247 | 8_1:+6724963 | Msa1175410:CDS |
| TAATGTTTCAAGCTCTGTGC+AGG | 0.524413 | 8_1:+6725087 | Msa1175410:CDS |
| GTCAATAAGCACTACACCAT+GGG | 0.524982 | 8_1:+6724811 | Msa1175410:CDS |
| GAAGGATATTGTTCCTGAGT+GGG | 0.526965 | 8_1:+6724958 | Msa1175410:CDS |
| ATATAACTATCAGGAAGTCC+TGG | 0.527887 | 8_1:-6724914 | None:intergenic |
| ATCGGTTTCTCAGCAGCCAC+TGG | 0.531685 | 8_1:+6724986 | Msa1175410:CDS |
| TATAGTACCAATGTTGCAGA+TGG | 0.532177 | 8_1:+6724599 | Msa1175410:CDS |
| TTTGGCCTTCTTCATTGCAC+CGG | 0.538662 | 8_1:+6724622 | Msa1175410:CDS |
| GATGAAGACTGTTAAGGAGA+TGG | 0.541381 | 8_1:-6724327 | None:intergenic |
| ATCGACCGGTGCAATGAAGA+AGG | 0.544745 | 8_1:-6724627 | None:intergenic |
| GTTCCTGAGTGGGTAAGGAT+CGG | 0.550149 | 8_1:+6724968 | Msa1175410:CDS |
| AGGTAGTTATGGCATTGCCT+TGG | 0.557576 | 8_1:-6724433 | None:intergenic |
| TTCTGCAAATTCTGCACCAG+TGG | 0.566800 | 8_1:-6725002 | None:intergenic |
| AAGTTGTAACTTCCCTGTTG+AGG | 0.568898 | 8_1:-6724453 | None:intergenic |
| ATAAGGAGCGGTTATGGCAA+AGG | 0.569731 | 8_1:-6724579 | None:intergenic |
| CAAAAGAATCTAATCTTCCA+AGG | 0.573374 | 8_1:+6724416 | Msa1175410:CDS |
| AAACCGATCCTTACCCACTC+AGG | 0.577788 | 8_1:-6724971 | None:intergenic |
| GTCTTGGCTAAACTTTGTGA+TGG | 0.583455 | 8_1:-6724393 | None:intergenic |
| TCAGGAACAATATCCTTCAA+AGG | 0.596202 | 8_1:-6724953 | None:intergenic |
| TGAAGGATATTGTTCCTGAG+TGG | 0.597859 | 8_1:+6724957 | Msa1175410:CDS |
| GATACTCAGCCACAAAACAT+CGG | 0.646194 | 8_1:+6724647 | Msa1175410:CDS |
| AGTCAATAAGCACTACACCA+TGG | 0.646654 | 8_1:+6724810 | Msa1175410:CDS |
| AACATTGGTACTATAAGGAG+CGG | 0.664620 | 8_1:-6724591 | None:intergenic |
| AGGGAAGTTACAACTTACCA+AGG | 0.684795 | 8_1:+6724460 | Msa1175410:CDS |
| GGATAGCAAAACAGGAGACG+TGG | 0.685503 | 8_1:+6724535 | Msa1175410:CDS |
| ATGCCATAACTACCTCAACA+GGG | 0.753652 | 8_1:+6724441 | Msa1175410:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATTGTTGTTATTAAAAACT+AGG | - | chr8_1:6725066-6725085 | None:intergenic | 20.0% |
| !!! | CATTGTTGTTATTAAAAACT+AGG | - | chr8_1:6725066-6725085 | None:intergenic | 20.0% |
| !!! | CTTCATCTTGTTATTCAATA+AGG | + | chr8_1:6724343-6724362 | Msa1175410:CDS | 25.0% |
| !! | TAGACACTTTTCATAATACT+TGG | + | chr8_1:6724741-6724760 | Msa1175410:CDS | 25.0% |
| !! | AGACACTTTTCATAATACTT+GGG | + | chr8_1:6724742-6724761 | Msa1175410:CDS | 25.0% |
| ! | CCAATATGTCTATTTATCTT+TGG | - | chr8_1:6724770-6724789 | None:intergenic | 25.0% |
| ! | CCAAAGATAAATAGACATAT+TGG | + | chr8_1:6724767-6724786 | Msa1175410:CDS | 25.0% |
| ! | TCACTAAGTATATAACTATC+AGG | - | chr8_1:6724926-6724945 | None:intergenic | 25.0% |
| !!! | CTTCATCTTGTTATTCAATA+AGG | + | chr8_1:6724343-6724362 | Msa1175410:CDS | 25.0% |
| !! | TAGACACTTTTCATAATACT+TGG | + | chr8_1:6724741-6724760 | Msa1175410:CDS | 25.0% |
| !! | AGACACTTTTCATAATACTT+GGG | + | chr8_1:6724742-6724761 | Msa1175410:CDS | 25.0% |
| ! | CCAATATGTCTATTTATCTT+TGG | - | chr8_1:6724770-6724789 | None:intergenic | 25.0% |
| ! | CCAAAGATAAATAGACATAT+TGG | + | chr8_1:6724767-6724786 | Msa1175410:CDS | 25.0% |
| ! | TCACTAAGTATATAACTATC+AGG | - | chr8_1:6724926-6724945 | None:intergenic | 25.0% |
| TAACAAGATGAAGACTGTTA+AGG | - | chr8_1:6724336-6724355 | None:intergenic | 30.0% | |
| ! | AGATTAGATTCTTTTGGTCT+TGG | - | chr8_1:6724412-6724431 | None:intergenic | 30.0% |
| ! | CTTGGAAGATTAGATTCTTT+TGG | - | chr8_1:6724418-6724437 | None:intergenic | 30.0% |
| CAAAAGAATCTAATCTTCCA+AGG | + | chr8_1:6724416-6724435 | Msa1175410:CDS | 30.0% | |
| CAGAACATGATATTCGTTAT+TGG | + | chr8_1:6725020-6725039 | Msa1175410:CDS | 30.0% | |
| TAACAAGATGAAGACTGTTA+AGG | - | chr8_1:6724336-6724355 | None:intergenic | 30.0% | |
| ! | AGATTAGATTCTTTTGGTCT+TGG | - | chr8_1:6724412-6724431 | None:intergenic | 30.0% |
| ! | CTTGGAAGATTAGATTCTTT+TGG | - | chr8_1:6724418-6724437 | None:intergenic | 30.0% |
| CAAAAGAATCTAATCTTCCA+AGG | + | chr8_1:6724416-6724435 | Msa1175410:CDS | 30.0% | |
| CAGAACATGATATTCGTTAT+TGG | + | chr8_1:6725020-6725039 | Msa1175410:CDS | 30.0% | |
| GCTAAACTTTGTGATGGTAA+AGG | - | chr8_1:6724390-6724409 | None:intergenic | 35.0% | |
| ATTCTGCTCCTATTCATATC+TGG | + | chr8_1:6724513-6724532 | Msa1175410:CDS | 35.0% | |
| TTCTGCTCCTATTCATATCT+GGG | + | chr8_1:6724514-6724533 | Msa1175410:CDS | 35.0% | |
| TTGCTATCCCAGATATGAAT+AGG | - | chr8_1:6724524-6724543 | None:intergenic | 35.0% | |
| AACATTGGTACTATAAGGAG+CGG | - | chr8_1:6724594-6724613 | None:intergenic | 35.0% | |
| TCTGCAACATTGGTACTATA+AGG | - | chr8_1:6724599-6724618 | None:intergenic | 35.0% | |
| TATAGTACCAATGTTGCAGA+TGG | + | chr8_1:6724599-6724618 | Msa1175410:CDS | 35.0% | |
| !!! | AGTGTAGTTTTGTCTTATCC+AGG | + | chr8_1:6724896-6724915 | Msa1175410:CDS | 35.0% |
| ATATAACTATCAGGAAGTCC+TGG | - | chr8_1:6724917-6724936 | None:intergenic | 35.0% | |
| TCAGGAACAATATCCTTCAA+AGG | - | chr8_1:6724956-6724975 | None:intergenic | 35.0% | |
| GCTAAACTTTGTGATGGTAA+AGG | - | chr8_1:6724390-6724409 | None:intergenic | 35.0% | |
| ATTCTGCTCCTATTCATATC+TGG | + | chr8_1:6724513-6724532 | Msa1175410:CDS | 35.0% | |
| TTCTGCTCCTATTCATATCT+GGG | + | chr8_1:6724514-6724533 | Msa1175410:CDS | 35.0% | |
| TTGCTATCCCAGATATGAAT+AGG | - | chr8_1:6724524-6724543 | None:intergenic | 35.0% | |
| AACATTGGTACTATAAGGAG+CGG | - | chr8_1:6724594-6724613 | None:intergenic | 35.0% | |
| TCTGCAACATTGGTACTATA+AGG | - | chr8_1:6724599-6724618 | None:intergenic | 35.0% | |
| TATAGTACCAATGTTGCAGA+TGG | + | chr8_1:6724599-6724618 | Msa1175410:CDS | 35.0% | |
| !!! | AGTGTAGTTTTGTCTTATCC+AGG | + | chr8_1:6724896-6724915 | Msa1175410:CDS | 35.0% |
| ATATAACTATCAGGAAGTCC+TGG | - | chr8_1:6724917-6724936 | None:intergenic | 35.0% | |
| TCAGGAACAATATCCTTCAA+AGG | - | chr8_1:6724956-6724975 | None:intergenic | 35.0% | |
| GATGAAGACTGTTAAGGAGA+TGG | - | chr8_1:6724330-6724349 | None:intergenic | 40.0% | |
| GTCTTGGCTAAACTTTGTGA+TGG | - | chr8_1:6724396-6724415 | None:intergenic | 40.0% | |
| AATGCCATAACTACCTCAAC+AGG | + | chr8_1:6724440-6724459 | Msa1175410:CDS | 40.0% | |
| ATGCCATAACTACCTCAACA+GGG | + | chr8_1:6724441-6724460 | Msa1175410:CDS | 40.0% | |
| AAGTTGTAACTTCCCTGTTG+AGG | - | chr8_1:6724456-6724475 | None:intergenic | 40.0% | |
| AGGGAAGTTACAACTTACCA+AGG | + | chr8_1:6724460-6724479 | Msa1175410:CDS | 40.0% | |
| AATAGAGTTCTTGACTGCCT+TGG | - | chr8_1:6724480-6724499 | None:intergenic | 40.0% | |
| AAGGCAGTCAAGAACTCTAT+TGG | + | chr8_1:6724479-6724498 | Msa1175410:CDS | 40.0% | |
| ! | CATATCTGGGATAGCAAAAC+AGG | + | chr8_1:6724527-6724546 | Msa1175410:CDS | 40.0% |
| TACCAATGTTGCAGATGGTT+TGG | + | chr8_1:6724604-6724623 | Msa1175410:CDS | 40.0% | |
| GATACTCAGCCACAAAACAT+CGG | + | chr8_1:6724647-6724666 | Msa1175410:CDS | 40.0% | |
| AGTCAATAAGCACTACACCA+TGG | + | chr8_1:6724810-6724829 | Msa1175410:CDS | 40.0% | |
| GTCAATAAGCACTACACCAT+GGG | + | chr8_1:6724811-6724830 | Msa1175410:CDS | 40.0% | |
| !!! | ACACCATGGGTTTTGGAAAA+TGG | + | chr8_1:6724824-6724843 | Msa1175410:CDS | 40.0% |
| ! | CTTCCATTTTCCAAAACCCA+TGG | - | chr8_1:6724830-6724849 | None:intergenic | 40.0% |
| !!! | GGTTTTGGAAAATGGAAGAG+AGG | + | chr8_1:6724832-6724851 | Msa1175410:CDS | 40.0% |
| GAGAGGCTAATGTTGTGATA+AGG | + | chr8_1:6724849-6724868 | Msa1175410:CDS | 40.0% | |
| TAGTGATGTTGTGCCTTTGA+AGG | + | chr8_1:6724940-6724959 | Msa1175410:CDS | 40.0% | |
| TGAAGGATATTGTTCCTGAG+TGG | + | chr8_1:6724957-6724976 | Msa1175410:CDS | 40.0% | |
| GAAGGATATTGTTCCTGAGT+GGG | + | chr8_1:6724958-6724977 | Msa1175410:CDS | 40.0% | |
| ATATTGTTCCTGAGTGGGTA+AGG | + | chr8_1:6724963-6724982 | Msa1175410:CDS | 40.0% | |
| TAATGTTTCAAGCTCTGTGC+AGG | + | chr8_1:6725087-6725106 | Msa1175410:CDS | 40.0% | |
| GATGAAGACTGTTAAGGAGA+TGG | - | chr8_1:6724330-6724349 | None:intergenic | 40.0% | |
| GTCTTGGCTAAACTTTGTGA+TGG | - | chr8_1:6724396-6724415 | None:intergenic | 40.0% | |
| AATGCCATAACTACCTCAAC+AGG | + | chr8_1:6724440-6724459 | Msa1175410:CDS | 40.0% | |
| ATGCCATAACTACCTCAACA+GGG | + | chr8_1:6724441-6724460 | Msa1175410:CDS | 40.0% | |
| AAGTTGTAACTTCCCTGTTG+AGG | - | chr8_1:6724456-6724475 | None:intergenic | 40.0% | |
| AGGGAAGTTACAACTTACCA+AGG | + | chr8_1:6724460-6724479 | Msa1175410:CDS | 40.0% | |
| AATAGAGTTCTTGACTGCCT+TGG | - | chr8_1:6724480-6724499 | None:intergenic | 40.0% | |
| AAGGCAGTCAAGAACTCTAT+TGG | + | chr8_1:6724479-6724498 | Msa1175410:CDS | 40.0% | |
| ! | CATATCTGGGATAGCAAAAC+AGG | + | chr8_1:6724527-6724546 | Msa1175410:CDS | 40.0% |
| TACCAATGTTGCAGATGGTT+TGG | + | chr8_1:6724604-6724623 | Msa1175410:CDS | 40.0% | |
| GATACTCAGCCACAAAACAT+CGG | + | chr8_1:6724647-6724666 | Msa1175410:CDS | 40.0% | |
| AGTCAATAAGCACTACACCA+TGG | + | chr8_1:6724810-6724829 | Msa1175410:CDS | 40.0% | |
| GTCAATAAGCACTACACCAT+GGG | + | chr8_1:6724811-6724830 | Msa1175410:CDS | 40.0% | |
| !!! | ACACCATGGGTTTTGGAAAA+TGG | + | chr8_1:6724824-6724843 | Msa1175410:CDS | 40.0% |
| ! | CTTCCATTTTCCAAAACCCA+TGG | - | chr8_1:6724830-6724849 | None:intergenic | 40.0% |
| !!! | GGTTTTGGAAAATGGAAGAG+AGG | + | chr8_1:6724832-6724851 | Msa1175410:CDS | 40.0% |
| GAGAGGCTAATGTTGTGATA+AGG | + | chr8_1:6724849-6724868 | Msa1175410:CDS | 40.0% | |
| TAGTGATGTTGTGCCTTTGA+AGG | + | chr8_1:6724940-6724959 | Msa1175410:CDS | 40.0% | |
| TGAAGGATATTGTTCCTGAG+TGG | + | chr8_1:6724957-6724976 | Msa1175410:CDS | 40.0% | |
| GAAGGATATTGTTCCTGAGT+GGG | + | chr8_1:6724958-6724977 | Msa1175410:CDS | 40.0% | |
| ATATTGTTCCTGAGTGGGTA+AGG | + | chr8_1:6724963-6724982 | Msa1175410:CDS | 40.0% | |
| TAATGTTTCAAGCTCTGTGC+AGG | + | chr8_1:6725087-6725106 | Msa1175410:CDS | 40.0% | |
| AGGTAGTTATGGCATTGCCT+TGG | - | chr8_1:6724436-6724455 | None:intergenic | 45.0% | |
| CTTCCCTGTTGAGGTAGTTA+TGG | - | chr8_1:6724447-6724466 | None:intergenic | 45.0% | |
| ATAAGGAGCGGTTATGGCAA+AGG | - | chr8_1:6724582-6724601 | None:intergenic | 45.0% | |
| GGTACTATAAGGAGCGGTTA+TGG | - | chr8_1:6724588-6724607 | None:intergenic | 45.0% | |
| ! | TTTGGCCTTCTTCATTGCAC+CGG | + | chr8_1:6724622-6724641 | Msa1175410:CDS | 45.0% |
| !!! | TTTTGTGGCTGAGTATCGAC+CGG | - | chr8_1:6724644-6724663 | None:intergenic | 45.0% |
| ! | AAGCACTACACCATGGGTTT+TGG | + | chr8_1:6724817-6724836 | Msa1175410:CDS | 45.0% |
| TTCTGCAAATTCTGCACCAG+TGG | - | chr8_1:6725005-6725024 | None:intergenic | 45.0% | |
| AGGTAGTTATGGCATTGCCT+TGG | - | chr8_1:6724436-6724455 | None:intergenic | 45.0% | |
| CTTCCCTGTTGAGGTAGTTA+TGG | - | chr8_1:6724447-6724466 | None:intergenic | 45.0% | |
| ATAAGGAGCGGTTATGGCAA+AGG | - | chr8_1:6724582-6724601 | None:intergenic | 45.0% | |
| GGTACTATAAGGAGCGGTTA+TGG | - | chr8_1:6724588-6724607 | None:intergenic | 45.0% | |
| ! | TTTGGCCTTCTTCATTGCAC+CGG | + | chr8_1:6724622-6724641 | Msa1175410:CDS | 45.0% |
| !!! | TTTTGTGGCTGAGTATCGAC+CGG | - | chr8_1:6724644-6724663 | None:intergenic | 45.0% |
| ! | AAGCACTACACCATGGGTTT+TGG | + | chr8_1:6724817-6724836 | Msa1175410:CDS | 45.0% |
| TTCTGCAAATTCTGCACCAG+TGG | - | chr8_1:6725005-6725024 | None:intergenic | 45.0% | |
| GGATAGCAAAACAGGAGACG+TGG | + | chr8_1:6724535-6724554 | Msa1175410:CDS | 50.0% | |
| GGCCAAACCATCTGCAACAT+TGG | - | chr8_1:6724609-6724628 | None:intergenic | 50.0% | |
| ATCGACCGGTGCAATGAAGA+AGG | - | chr8_1:6724630-6724649 | None:intergenic | 50.0% | |
| ATCGGTCGTGCAGGATTTCT+CGG | + | chr8_1:6724665-6724684 | Msa1175410:CDS | 50.0% | |
| GTTCCTGAGTGGGTAAGGAT+CGG | + | chr8_1:6724968-6724987 | Msa1175410:CDS | 50.0% | |
| AAACCGATCCTTACCCACTC+AGG | - | chr8_1:6724974-6724993 | None:intergenic | 50.0% | |
| GGATAGCAAAACAGGAGACG+TGG | + | chr8_1:6724535-6724554 | Msa1175410:CDS | 50.0% | |
| GGCCAAACCATCTGCAACAT+TGG | - | chr8_1:6724609-6724628 | None:intergenic | 50.0% | |
| ATCGACCGGTGCAATGAAGA+AGG | - | chr8_1:6724630-6724649 | None:intergenic | 50.0% | |
| ATCGGTCGTGCAGGATTTCT+CGG | + | chr8_1:6724665-6724684 | Msa1175410:CDS | 50.0% | |
| GTTCCTGAGTGGGTAAGGAT+CGG | + | chr8_1:6724968-6724987 | Msa1175410:CDS | 50.0% | |
| AAACCGATCCTTACCCACTC+AGG | - | chr8_1:6724974-6724993 | None:intergenic | 50.0% | |
| ! | CCTGCACGACCGATGTTTTG+TGG | - | chr8_1:6724659-6724678 | None:intergenic | 55.0% |
| CCACAAAACATCGGTCGTGC+AGG | + | chr8_1:6724656-6724675 | Msa1175410:CDS | 55.0% | |
| ! | ATCGGTTTCTCAGCAGCCAC+TGG | + | chr8_1:6724986-6725005 | Msa1175410:CDS | 55.0% |
| ! | CCTGCACGACCGATGTTTTG+TGG | - | chr8_1:6724659-6724678 | None:intergenic | 55.0% |
| CCACAAAACATCGGTCGTGC+AGG | + | chr8_1:6724656-6724675 | Msa1175410:CDS | 55.0% | |
| ! | ATCGGTTTCTCAGCAGCCAC+TGG | + | chr8_1:6724986-6725005 | Msa1175410:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 6724293 | 6725117 | 6724293 | ID=Msa1175410;Name=Msa1175410 |
| chr8_1 | mRNA | 6724293 | 6725117 | 6724293 | ID=Msa1175410-mRNA-1;Parent=Msa1175410;Name=Msa1175410-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|274 |
| chr8_1 | exon | 6724293 | 6725117 | 6724293 | ID=Msa1175410-mRNA-1:exon:2386;Parent=Msa1175410-mRNA-1 |
| chr8_1 | CDS | 6724293 | 6725117 | 6724293 | ID=Msa1175410-mRNA-1:cds;Parent=Msa1175410-mRNA-1 |
| Gene Sequence |
| Protein sequence |
