AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1177520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1177520 MtrunA17_Chr8g0348131 97.004 267 6 1 1 265 1 267 0.0 512
Msa1177520 MtrunA17_Chr8g0387631 65.414 266 80 3 1 263 1 257 3.25e-113 326
Msa1177520 MtrunA17_Chr1g0202401 57.627 236 98 1 18 251 7 242 2.07e-93 275
Msa1177520 MtrunA17_Chr3g0095441 44.000 225 124 1 22 244 3 227 7.88e-63 197
Msa1177520 MtrunA17_Chr5g0442361 44.286 210 111 2 51 258 33 238 1.30e-56 181
Msa1177520 MtrunA17_Chr8g0376101 35.345 232 135 2 32 263 4 220 7.37e-47 155
Msa1177520 MtrunA17_Chr1g0209261 37.255 204 118 3 60 263 10 203 2.22e-43 146
Msa1177520 MtrunA17_Chr3g0108661 35.652 230 133 2 28 250 16 237 5.27e-41 141
Msa1177520 MtrunA17_Chr4g0005701 34.197 193 127 0 59 251 6 198 1.21e-32 119
Msa1177520 MtrunA17_Chr5g0402811 36.413 184 117 0 72 255 29 212 1.23e-30 114
Msa1177520 MtrunA17_Chr6g0453331 35.359 181 117 0 77 257 14 194 8.26e-28 105
Msa1177520 MtrunA17_Chr1g0208091 34.171 199 127 1 67 265 9 203 1.20e-22 92.4
Msa1177520 MtrunA17_Chr5g0402701 38.519 135 83 0 81 215 346 480 1.10e-19 88.2
Msa1177520 MtrunA17_Chr3g0136511 33.333 177 96 5 63 226 368 535 4.06e-13 68.9
Msa1177520 MtrunA17_Chr5g0402691 33.582 134 88 1 81 213 283 416 2.20e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1177520 AT2G46170.1 60.837 263 91 3 1 263 1 251 1.10e-108 315
Msa1177520 AT4G23630.1 71.498 207 55 1 57 263 70 272 9.12e-108 313
Msa1177520 AT4G23630.2 71.498 207 55 1 57 263 70 272 9.12e-108 313
Msa1177520 AT5G41600.1 62.698 252 93 1 13 263 4 255 1.66e-105 306
Msa1177520 AT1G64090.1 63.900 241 84 1 18 258 9 246 1.59e-103 301
Msa1177520 AT4G11220.1 58.456 272 100 3 1 263 1 268 5.33e-103 301
Msa1177520 AT3G61560.1 59.245 265 96 3 1 265 1 253 1.98e-100 293
Msa1177520 AT3G61560.2 59.245 265 96 3 1 265 1 253 1.98e-100 293
Msa1177520 AT1G64090.2 59.533 257 85 2 18 258 9 262 2.89e-98 289
Msa1177520 AT3G61560.3 59.420 207 76 2 1 207 1 199 4.42e-77 233
Msa1177520 AT2G46170.2 58.919 185 68 2 1 185 1 177 4.62e-70 214
Msa1177520 AT4G01230.2 50.754 199 95 1 53 251 47 242 1.34e-66 207
Msa1177520 AT4G01230.1 49.246 199 96 2 53 251 47 240 4.08e-62 196
Msa1177520 AT3G10260.1 42.786 201 115 0 51 251 36 236 9.22e-60 190
Msa1177520 AT3G10260.2 42.786 201 115 0 51 251 36 236 9.22e-60 190
Msa1177520 AT3G10260.3 42.786 201 115 0 51 251 56 256 1.39e-59 190
Msa1177520 AT3G18260.1 36.957 230 131 2 34 263 7 222 1.92e-50 165
Msa1177520 AT3G10915.2 31.963 219 145 2 33 251 2 216 6.95e-37 130
Msa1177520 AT2G15280.1 34.536 194 121 2 64 254 2 192 7.17e-37 129
Msa1177520 AT3G10915.7 31.959 194 132 0 58 251 48 241 1.95e-35 127
Msa1177520 AT3G10915.4 31.818 220 145 3 33 251 2 217 3.15e-35 126
Msa1177520 AT1G68230.2 37.436 195 112 3 71 263 26 212 2.97e-33 120
Msa1177520 AT3G10915.5 28.629 248 150 4 33 257 2 245 1.39e-29 112
Msa1177520 AT3G19460.1 35.849 159 102 0 68 226 14 172 1.63e-29 110
Msa1177520 AT3G19460.5 35.849 159 102 0 68 226 14 172 1.63e-29 110
Msa1177520 AT3G19460.4 35.849 159 102 0 68 226 14 172 1.63e-29 110
Msa1177520 AT3G10915.6 29.545 176 124 0 76 251 9 184 1.12e-26 103
Msa1177520 AT3G10915.3 29.545 176 124 0 76 251 8 183 2.24e-26 102
Msa1177520 AT3G54120.1 35.329 167 108 0 59 225 7 173 2.85e-24 97.1
Msa1177520 AT3G19460.3 30.052 193 101 1 68 226 14 206 5.17e-23 94.4
Msa1177520 AT3G19460.7 30.052 193 101 1 68 226 14 206 5.17e-23 94.4
Msa1177520 AT1G68230.1 38.406 138 79 2 71 206 26 159 2.98e-21 87.8
Msa1177520 AT3G19460.2 29.348 184 96 1 77 226 7 190 1.47e-19 84.7
Msa1177520 AT3G19460.6 29.348 184 96 1 77 226 7 190 1.47e-19 84.7
Msa1177520 AT2G23640.1 26.316 171 126 0 78 248 18 188 2.28e-19 84.0
Msa1177520 AT3G10915.1 30.719 153 102 2 33 185 2 150 2.78e-18 80.1
Msa1177520 AT2G15280.2 29.915 117 82 0 64 180 2 118 1.27e-17 78.2
Msa1177520 AT2G01240.1 31.655 139 85 3 121 258 1 130 1.70e-11 60.8

Find 66 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTGTCACATTTCGATTC+TGG 0.146999 8_1:-9896155 Msa1177520:CDS
TTGGGACAGCAACTGCTATT+TGG 0.161400 8_1:-9896216 Msa1177520:CDS
TTTCCTTTCAACACACCTTT+TGG 0.257041 8_1:+9895127 None:intergenic
TTCTGATTCTCATTCTGATT+TGG 0.273750 8_1:-9897145 Msa1177520:CDS
TTTGATTGATTAAAATATTT+GGG 0.294747 8_1:-9894869 Msa1177520:three_prime_UTR
TAGTCCTTGATTAACTTTGC+TGG 0.325176 8_1:-9894929 Msa1177520:three_prime_UTR
GATTTATTGGCTACTCTGTT+TGG 0.331987 8_1:-9894960 Msa1177520:three_prime_UTR
TGGGACAGCAACTGCTATTT+GGG 0.332755 8_1:-9896215 Msa1177520:CDS
TTAAAATAACACGAATCATC+AGG 0.341305 8_1:+9894805 None:intergenic
AGAGAGTGTAGTGTGGTTAG+AGG 0.352208 8_1:+9897352 None:intergenic
CAGGCTTCTCCCTTCCAAAA+AGG 0.354297 8_1:+9897079 None:intergenic
TCCTTCACCGATTCAATAAC+CGG 0.358423 8_1:+9897114 None:intergenic
CACCGAGAAGCTTCACTTTG+AGG 0.367936 8_1:-9897184 Msa1177520:CDS
TTCTAGGCAGTATTGATACT+TGG 0.392877 8_1:-9895011 Msa1177520:three_prime_UTR
ATCCTCAAAGTGAAGCTTCT+CGG 0.394266 8_1:+9897182 None:intergenic
TATTCTTACATATGTAGAAC+AGG 0.397072 8_1:+9895375 None:intergenic
GCAGAAGCAATCTGCAGAAA+TGG 0.411515 8_1:+9895926 None:intergenic
TTCTGATTTGGATAAGAAAC+CGG 0.414962 8_1:-9897133 Msa1177520:CDS
TCAACGAAACCCTAAATCTC+AGG 0.422908 8_1:+9897281 None:intergenic
TCCTCAGGAAGATGAACAAT+TGG 0.425388 8_1:+9895950 None:intergenic
TGTGGATTCTGTCGATTGTT+GGG 0.429665 8_1:-9895418 Msa1177520:CDS
TTCTGTCGATTGTTGGGAGC+TGG 0.437564 8_1:-9895412 Msa1177520:CDS
TTGTGGATTCTGTCGATTGT+TGG 0.438890 8_1:-9895419 Msa1177520:CDS
ATCTGCAGAAATGGTTCCTC+AGG 0.452475 8_1:+9895935 None:intergenic
GCCTTGCGTGATATTGGTAC+TGG 0.456671 8_1:-9895872 Msa1177520:CDS
GATAGACCCTCTGGCAGAAA+AGG 0.457105 8_1:-9895221 Msa1177520:CDS
GTTCACTCCGTCCTCGGCGG+CGG 0.462690 8_1:-9897057 Msa1177520:CDS
CCGCCGAGGACGGAGTGAAC+AGG 0.464754 8_1:+9897060 None:intergenic
GTATGCTGTGTTTGACGAAA+AGG 0.466260 8_1:-9895176 Msa1177520:CDS
AAGTTATCTGCTGGTACACT+TGG 0.483724 8_1:-9896235 Msa1177520:CDS
TAAGAAACCGGTTATTGAAT+CGG 0.485712 8_1:-9897121 Msa1177520:CDS
CAGCTGCTGATGTGTTCTTG+TGG 0.506480 8_1:-9896267 Msa1177520:CDS
TCTAGGCAGTATTGATACTT+GGG 0.510592 8_1:-9895010 Msa1177520:three_prime_UTR
ATCTTCAGCAAGATCCCAAA+AGG 0.519575 8_1:-9895142 Msa1177520:CDS
ATCAGAATGAGAATCAGAAT+CGG 0.525151 8_1:+9897149 None:intergenic
ACGCTTTCCGCCGCCGAGGA+CGG 0.537634 8_1:+9897050 None:intergenic
CATACCAGCAAAGTTAATCA+AGG 0.542205 8_1:+9894925 None:intergenic
ATCCCAAAAGGTGTGTTGAA+AGG 0.543000 8_1:-9895130 Msa1177520:CDS
TATTGAATCGGTGAAGGAGA+AGG 0.548952 8_1:-9897109 Msa1177520:CDS
TAAAATAACACGAATCATCA+GGG 0.552238 8_1:+9894806 None:intergenic
AAGCCTGTTCACTCCGTCCT+CGG 0.554597 8_1:-9897063 Msa1177520:CDS
GTGAACAAAGGTATGAGCAT+TGG 0.557082 8_1:+9896108 None:intergenic
CACGCAAGGCAGAAAATCCT+CGG 0.571310 8_1:+9895885 None:intergenic
TCCAGTACCAATATCACGCA+AGG 0.574766 8_1:+9895871 None:intergenic
AGTTTGTAGAATGTGTGCTC+CGG 0.579104 8_1:-9895094 Msa1177520:three_prime_UTR
GGAGAAAAGCACCGTGACGG+TGG 0.582541 8_1:-9897241 Msa1177520:CDS
TCCAATTGTTCATCTTCCTG+AGG 0.588534 8_1:-9895951 Msa1177520:CDS
AAGAACAAGAAGTTATCTGC+TGG 0.588642 8_1:-9896244 Msa1177520:CDS
CGAGGACAAGATAGACCCTC+TGG 0.598470 8_1:-9895230 Msa1177520:CDS
ACCGGTTATTGAATCGGTGA+AGG 0.598940 8_1:-9897115 Msa1177520:CDS
GCCGTCACGATCCACCGTCA+CGG 0.610496 8_1:+9897230 None:intergenic
AGTTATCTGCTGGTACACTT+GGG 0.615512 8_1:-9896234 Msa1177520:CDS
ATCGGAGAAAAGCACCGTGA+CGG 0.632245 8_1:-9897244 Msa1177520:CDS
TGAACAATTGGAATGTGAGG+TGG 0.647379 8_1:+9895962 None:intergenic
AGAGTCTTACTTGTGAACAA+AGG 0.648694 8_1:+9896096 None:intergenic
TAGAGGCGAGAGTCTCAGTG+TGG 0.649895 8_1:+9897369 None:intergenic
AGAGGCGAGAGTCTCAGTGT+GGG 0.653226 8_1:+9897370 None:intergenic
TCGTACTTGTCATACACGAA+AGG 0.653781 8_1:+9895250 None:intergenic
ATTCTTACATATGTAGAACA+GGG 0.654822 8_1:+9895376 None:intergenic
TTGAGAATTGAAATCAACCG+AGG 0.656314 8_1:-9895902 Msa1177520:CDS
ACCGTGACGGTGGATCGTGA+CGG 0.663353 8_1:-9897231 Msa1177520:CDS
GTGACGGTGGATCGTGACGG+CGG 0.663536 8_1:-9897228 Msa1177520:CDS
AGATGAACAATTGGAATGTG+AGG 0.665708 8_1:+9895959 None:intergenic
TCATACGCTTTCCGCCGCCG+AGG 0.684893 8_1:+9897046 None:intergenic
CCTGTTCACTCCGTCCTCGG+CGG 0.706578 8_1:-9897060 Msa1177520:CDS
TTTCGTGTATGACAAGTACG+AGG 0.772122 8_1:-9895248 Msa1177520:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTTAAGTAATTATAGT+TGG + chr8_1:9896453-9896472 None:intergenic 10.0%
!!! TTTTGATTGATTAAAATATT+TGG - chr8_1:9897280-9897299 Msa1177520:five_prime_UTR 10.0%
!! TTTGATTGATTAAAATATTT+GGG - chr8_1:9897281-9897300 Msa1177520:five_prime_UTR 10.0%
!! AAGAAAAAAAAATTCTCTTT+CGG + chr8_1:9894830-9894849 None:intergenic 15.0%
!! AAAAAAATATCTATGAATCA+TGG - chr8_1:9895511-9895530 Msa1177520:intron 15.0%
!! ACAAAACAAGAAAAAATAAT+TGG + chr8_1:9895566-9895585 None:intergenic 15.0%
!!! AATATTTTGTAATGTTTTGT+AGG - chr8_1:9896162-9896181 Msa1177520:CDS 15.0%
!!! GTAGTTTAAAATTTTGAAAA+TGG - chr8_1:9897168-9897187 Msa1177520:CDS 15.0%
!!! AATTTTGAAAATGGATTTAT+TGG - chr8_1:9897177-9897196 Msa1177520:CDS 15.0%
!!! GTGTTATTTTAAAAATATCT+TGG - chr8_1:9897356-9897375 Msa1177520:five_prime_UTR 15.0%
!!! TTTTAAAAATATCTTGGTAA+AGG - chr8_1:9897362-9897381 Msa1177520:five_prime_UTR 15.0%
!!! CTTTATTGTTTTAGTTGTTT+TGG - chr8_1:9895129-9895148 Msa1177520:CDS 20.0%
!!! GATTCATAGATATTTTTTTG+AGG + chr8_1:9895510-9895529 None:intergenic 20.0%
!!! ATTCTTACTTTTTAGTGTTA+TGG - chr8_1:9895735-9895754 Msa1177520:intron 20.0%
!!! GTCTTAACTGTTTTTATAAT+TGG - chr8_1:9897101-9897120 Msa1177520:CDS 20.0%
!!! TTTTATAATTGGCCTTTTTT+TGG - chr8_1:9897112-9897131 Msa1177520:CDS 20.0%
!! AAAAAATTCTCTTTCGGTTT+TGG + chr8_1:9894824-9894843 None:intergenic 25.0%
! ATGTGAATGAAAATTGTGAT+TGG - chr8_1:9895210-9895229 Msa1177520:CDS 25.0%
!! GACTAGCTAACATAAATAAT+TGG + chr8_1:9895537-9895556 None:intergenic 25.0%
! ATTTCGTAGATACAATTCAA+TGG + chr8_1:9895619-9895638 None:intergenic 25.0%
! TGAATTGTATCTACGAAATT+TGG - chr8_1:9895620-9895639 Msa1177520:intron 25.0%
! TTATGTAAGATGTCAAAACA+TGG - chr8_1:9895693-9895712 Msa1177520:intron 25.0%
!! AATTTCATCTCTTCTTTTAG+AGG + chr8_1:9895812-9895831 None:intergenic 25.0%
! TTCATAAGTGAATGAATGAT+GGG - chr8_1:9896414-9896433 Msa1177520:intron 25.0%
!! ATGTTGTGTTAGGTAATTTT+TGG - chr8_1:9896707-9896726 Msa1177520:intron 25.0%
! ATTCTTACATATGTAGAACA+GGG + chr8_1:9896777-9896796 None:intergenic 25.0%
! TATTCTTACATATGTAGAAC+AGG + chr8_1:9896778-9896797 None:intergenic 25.0%
! AACGTATAAATCAGTTTGAT+AGG + chr8_1:9896804-9896823 None:intergenic 25.0%
!!! ATACTGTTTTTTAATCTCGA+TGG + chr8_1:9896958-9896977 None:intergenic 25.0%
! TAAAATAACACGAATCATCA+GGG + chr8_1:9897347-9897366 None:intergenic 25.0%
! TTAAAATAACACGAATCATC+AGG + chr8_1:9897348-9897367 None:intergenic 25.0%
!!! AACCTTTTTTCCTGAGATTT+AGG - chr8_1:9894859-9894878 Msa1177520:three_prime_UTR 30.0%
!!! ACCTTTTTTCCTGAGATTTA+GGG - chr8_1:9894860-9894879 Msa1177520:three_prime_UTR 30.0%
! TTTAGGGTTTCGTTGAAAAA+TGG - chr8_1:9894876-9894895 Msa1177520:three_prime_UTR 30.0%
ATCAGAATGAGAATCAGAAT+CGG + chr8_1:9895004-9895023 None:intergenic 30.0%
TTCTGATTCTCATTCTGATT+TGG - chr8_1:9895005-9895024 Msa1177520:three_prime_UTR 30.0%
!! TTCTGATTTGGATAAGAAAC+CGG - chr8_1:9895017-9895036 Msa1177520:three_prime_UTR 30.0%
TAAGAAACCGGTTATTGAAT+CGG - chr8_1:9895029-9895048 Msa1177520:three_prime_UTR 30.0%
TGTATCTACGAAATTTGGTT+TGG - chr8_1:9895625-9895644 Msa1177520:intron 30.0%
TGTCAAAACATGGTAATTTG+TGG - chr8_1:9895703-9895722 Msa1177520:intron 30.0%
ATAAAAACAGAGAACCAACT+CGG + chr8_1:9895766-9895785 None:intergenic 30.0%
!!! AGTTGGTTCTCTGTTTTTAT+TGG - chr8_1:9895766-9895785 Msa1177520:intron 30.0%
ATTATTGCGTATTTGTCTGA+TGG - chr8_1:9895846-9895865 Msa1177520:intron 30.0%
TTATTGCGTATTTGTCTGAT+GGG - chr8_1:9895847-9895866 Msa1177520:CDS 30.0%
!!! TTCTTGCACTATTGTTTTTG+TGG - chr8_1:9896018-9896037 Msa1177520:intron 30.0%
! TTATTGACTAGAAAGTTCAC+AGG + chr8_1:9896337-9896356 None:intergenic 30.0%
CTTCATAAGTGAATGAATGA+TGG - chr8_1:9896413-9896432 Msa1177520:intron 30.0%
TCATAAGTGAATGAATGATG+GGG - chr8_1:9896415-9896434 Msa1177520:intron 30.0%
CTTCATAAGTGAATGAATGA+CGG - chr8_1:9896413-9896432 Msa1177520:intron 30.0%
TTCATAAGTGAATGAATGAC+GGG - chr8_1:9896538-9896557 Msa1177520:intron 30.0%
!! TTTGTTGTTGATGTTGTGTT+AGG - chr8_1:9896697-9896716 Msa1177520:intron 30.0%
!!! AGCTTTTGTTTTGTTGTTCA+CGG - chr8_1:9896875-9896894 Msa1177520:intron 30.0%
ACCCTAAATCTCAGGAAAAA+AGG + chr8_1:9894864-9894883 None:intergenic 35.0%
TTGAAAAATGGCTGACGAAT+CGG - chr8_1:9894888-9894907 Msa1177520:three_prime_UTR 35.0%
! CTTTACCTCTGACCTAATTT+TGG - chr8_1:9895474-9895493 Msa1177520:intron 35.0%
GTAATCCAAAATTAGGTCAG+AGG + chr8_1:9895482-9895501 None:intergenic 35.0%
GGCACTAGTAATCCAAAATT+AGG + chr8_1:9895489-9895508 None:intergenic 35.0%
AAGAACAAGAAGTTATCTGC+TGG - chr8_1:9895906-9895925 Msa1177520:CDS 35.0%
CATTTGTCACATTTCGATTC+TGG - chr8_1:9895995-9896014 Msa1177520:intron 35.0%
AGAGTCTTACTTGTGAACAA+AGG + chr8_1:9896057-9896076 None:intergenic 35.0%
TATTCATCACATGCAGCAAA+TGG + chr8_1:9896136-9896155 None:intergenic 35.0%
AGATGAACAATTGGAATGTG+AGG + chr8_1:9896194-9896213 None:intergenic 35.0%
TTGAGAATTGAAATCAACCG+AGG - chr8_1:9896248-9896267 Msa1177520:CDS 35.0%
CATAAGTGAATGAATGATGG+GGG - chr8_1:9896416-9896435 Msa1177520:intron 35.0%
TCATAAGTGAATGAATGACG+GGG - chr8_1:9896539-9896558 Msa1177520:intron 35.0%
! CATGTTATTGACTACTGCTA+AGG - chr8_1:9896672-9896691 Msa1177520:intron 35.0%
!!! GTTAGGTAATTTTTGGCTTG+TGG - chr8_1:9896714-9896733 Msa1177520:intron 35.0%
! TTCCTTTCAACACACCTTTT+GGG + chr8_1:9897025-9897044 None:intergenic 35.0%
! TTTCCTTTCAACACACCTTT+TGG + chr8_1:9897026-9897045 None:intergenic 35.0%
!!! GCCTTTTTTTGGATTGTTCT+AGG - chr8_1:9897123-9897142 Msa1177520:CDS 35.0%
GCCTAGAACAATCCAAAAAA+AGG + chr8_1:9897127-9897146 None:intergenic 35.0%
!! TTCTAGGCAGTATTGATACT+TGG - chr8_1:9897139-9897158 Msa1177520:CDS 35.0%
!! TCTAGGCAGTATTGATACTT+GGG - chr8_1:9897140-9897159 Msa1177520:CDS 35.0%
! GATTTATTGGCTACTCTGTT+TGG - chr8_1:9897190-9897209 Msa1177520:CDS 35.0%
TAGTCCTTGATTAACTTTGC+TGG - chr8_1:9897221-9897240 Msa1177520:CDS 35.0%
CATACCAGCAAAGTTAATCA+AGG + chr8_1:9897228-9897247 None:intergenic 35.0%
TTCGTGCTGTGAAAAATGTT+TGG - chr8_1:9897314-9897333 Msa1177520:five_prime_UTR 35.0%
GCTGTGAAAAATGTTTGGAT+AGG - chr8_1:9897319-9897338 Msa1177520:five_prime_UTR 35.0%
TCAACGAAACCCTAAATCTC+AGG + chr8_1:9894872-9894891 None:intergenic 40.0%
ATCCTCAAAGTGAAGCTTCT+CGG + chr8_1:9894971-9894990 None:intergenic 40.0%
!! TCCTTCACCGATTCAATAAC+CGG + chr8_1:9895039-9895058 None:intergenic 40.0%
TATTGAATCGGTGAAGGAGA+AGG - chr8_1:9895041-9895060 Msa1177520:three_prime_UTR 40.0%
! GAGAAGGTTTTTCGCCTTTT+TGG - chr8_1:9895057-9895076 Msa1177520:three_prime_UTR 40.0%
! AGGTTTTTCGCCTTTTTGGA+AGG - chr8_1:9895061-9895080 Msa1177520:three_prime_UTR 40.0%
!! GGTTTTTCGCCTTTTTGGAA+GGG - chr8_1:9895062-9895081 Msa1177520:three_prime_UTR 40.0%
GCTCTTAATGACTTTGCCAA+AGG - chr8_1:9895395-9895414 Msa1177520:CDS 40.0%
TTGCCAAAGGACGAATTGTT+TGG - chr8_1:9895408-9895427 Msa1177520:CDS 40.0%
TGCCAAAGGACGAATTGTTT+GGG - chr8_1:9895409-9895428 Msa1177520:CDS 40.0%
TTCCCAAACAATTCGTCCTT+TGG + chr8_1:9895414-9895433 None:intergenic 40.0%
TTCTACACACAAATCGCACT+CGG + chr8_1:9895439-9895458 None:intergenic 40.0%
AAGTTATCTGCTGGTACACT+TGG - chr8_1:9895915-9895934 Msa1177520:CDS 40.0%
AGTTATCTGCTGGTACACTT+GGG - chr8_1:9895916-9895935 Msa1177520:CDS 40.0%
! GTGAACAAAGGTATGAGCAT+TGG + chr8_1:9896045-9896064 None:intergenic 40.0%
CATGCAGCAAATGGTTAGAT+AGG + chr8_1:9896127-9896146 None:intergenic 40.0%
TGAACAATTGGAATGTGAGG+TGG + chr8_1:9896191-9896210 None:intergenic 40.0%
TCCAATTGTTCATCTTCCTG+AGG - chr8_1:9896199-9896218 Msa1177520:CDS 40.0%
TCCTCAGGAAGATGAACAAT+TGG + chr8_1:9896203-9896222 None:intergenic 40.0%
! TTTTCTGCCTTGCGTGATAT+TGG - chr8_1:9896272-9896291 Msa1177520:intron 40.0%
TTCACAGGTTACGTACAATG+AGG + chr8_1:9896322-9896341 None:intergenic 40.0%
CATAAGTGAATGAATGACGG+GGG - chr8_1:9896540-9896559 Msa1177520:intron 40.0%
! ATAAGTGAATGAATGACGGG+GGG - chr8_1:9896541-9896560 Msa1177520:intron 40.0%
! TTGTGGATTCTGTCGATTGT+TGG - chr8_1:9896731-9896750 Msa1177520:intron 40.0%
! TGTGGATTCTGTCGATTGTT+GGG - chr8_1:9896732-9896751 Msa1177520:intron 40.0%
TCGTACTTGTCATACACGAA+AGG + chr8_1:9896903-9896922 None:intergenic 40.0%
TTTCGTGTATGACAAGTACG+AGG - chr8_1:9896902-9896921 Msa1177520:intron 40.0%
GTATGCTGTGTTTGACGAAA+AGG - chr8_1:9896974-9896993 Msa1177520:intron 40.0%
ATCTTCAGCAAGATCCCAAA+AGG - chr8_1:9897008-9897027 Msa1177520:intron 40.0%
! ATCCCAAAAGGTGTGTTGAA+AGG - chr8_1:9897020-9897039 Msa1177520:intron 40.0%
AGTTTGTAGAATGTGTGCTC+CGG - chr8_1:9897056-9897075 Msa1177520:CDS 40.0%
!!! AGCCTCTTTTTTCTTCTAGC+CGG + chr8_1:9897078-9897097 None:intergenic 40.0%
! AGAGAGTGTAGTGTGGTTAG+AGG + chr8_1:9894801-9894820 None:intergenic 45.0%
!!! GTTTTGGAGAGAGTGTAGTG+TGG + chr8_1:9894808-9894827 None:intergenic 45.0%
ACCGGTTATTGAATCGGTGA+AGG - chr8_1:9895035-9895054 Msa1177520:three_prime_UTR 45.0%
!! GTGTTATGGTTTTGCCGAGT+TGG - chr8_1:9895749-9895768 Msa1177520:intron 45.0%
TTGGGACAGCAACTGCTATT+TGG - chr8_1:9895934-9895953 Msa1177520:CDS 45.0%
! TGGGACAGCAACTGCTATTT+GGG - chr8_1:9895935-9895954 Msa1177520:CDS 45.0%
ATCTGCAGAAATGGTTCCTC+AGG + chr8_1:9896218-9896237 None:intergenic 45.0%
GCAGAAGCAATCTGCAGAAA+TGG + chr8_1:9896227-9896246 None:intergenic 45.0%
TCCAGTACCAATATCACGCA+AGG + chr8_1:9896282-9896301 None:intergenic 45.0%
CTCCGGCTAGAAGAAAAAAG+AGG - chr8_1:9897073-9897092 Msa1177520:CDS 45.0%
!! ATCGGAGAAAAGCACCGTGA+CGG - chr8_1:9894906-9894925 Msa1177520:three_prime_UTR 50.0%
CACCGAGAAGCTTCACTTTG+AGG - chr8_1:9894966-9894985 Msa1177520:three_prime_UTR 50.0%
CAGGCTTCTCCCTTCCAAAA+AGG + chr8_1:9895074-9895093 None:intergenic 50.0%
CAGCTGCTGATGTGTTCTTG+TGG - chr8_1:9895883-9895902 Msa1177520:CDS 50.0%
CACGCAAGGCAGAAAATCCT+CGG + chr8_1:9896268-9896287 None:intergenic 50.0%
GCCTTGCGTGATATTGGTAC+TGG - chr8_1:9896278-9896297 Msa1177520:intron 50.0%
! TTCTGTCGATTGTTGGGAGC+TGG - chr8_1:9896738-9896757 Msa1177520:intron 50.0%
GATAGACCCTCTGGCAGAAA+AGG - chr8_1:9896929-9896948 Msa1177520:intron 50.0%
! TGGATGCCTTTTCTGCCAGA+GGG + chr8_1:9896938-9896957 None:intergenic 50.0%
! ATGGATGCCTTTTCTGCCAG+AGG + chr8_1:9896939-9896958 None:intergenic 50.0%
AGAGGCGAGAGTCTCAGTGT+GGG + chr8_1:9894783-9894802 None:intergenic 55.0%
TAGAGGCGAGAGTCTCAGTG+TGG + chr8_1:9894784-9894803 None:intergenic 55.0%
AAGCCTGTTCACTCCGTCCT+CGG - chr8_1:9895087-9895106 Msa1177520:three_prime_UTR 55.0%
CGAGGACAAGATAGACCCTC+TGG - chr8_1:9896920-9896939 Msa1177520:intron 55.0%
!! GGAGAAAAGCACCGTGACGG+TGG - chr8_1:9894909-9894928 Msa1177520:three_prime_UTR 60.0%
ACCGTGACGGTGGATCGTGA+CGG - chr8_1:9894919-9894938 Msa1177520:three_prime_UTR 60.0%
GCCGTCACGATCCACCGTCA+CGG + chr8_1:9894923-9894942 None:intergenic 65.0%
GTGACGGTGGATCGTGACGG+CGG - chr8_1:9894922-9894941 Msa1177520:three_prime_UTR 65.0%
CCTGTTCACTCCGTCCTCGG+CGG - chr8_1:9895090-9895109 Msa1177520:three_prime_UTR 65.0%
TCATACGCTTTCCGCCGCCG+AGG + chr8_1:9895107-9895126 None:intergenic 65.0%
CCGCCGAGGACGGAGTGAAC+AGG + chr8_1:9895093-9895112 None:intergenic 70.0%
GTTCACTCCGTCCTCGGCGG+CGG - chr8_1:9895093-9895112 Msa1177520:three_prime_UTR 70.0%
ACGCTTTCCGCCGCCGAGGA+CGG + chr8_1:9895103-9895122 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 9894764 9897408 9894764 ID=Msa1177520;Name=Msa1177520
chr8_1 mRNA 9894764 9897408 9894764 ID=Msa1177520-mRNA-1;Parent=Msa1177520;Name=Msa1177520-mRNA-1;_AED=0.06;_eAED=0.06;_QI=131|1|1|1|1|1|5|353|265
chr8_1 exon 9894764 9895295 9894764 ID=Msa1177520-mRNA-1:exon:3425;Parent=Msa1177520-mRNA-1
chr8_1 exon 9895384 9895453 9895384 ID=Msa1177520-mRNA-1:exon:3424;Parent=Msa1177520-mRNA-1
chr8_1 exon 9895847 9895988 9895847 ID=Msa1177520-mRNA-1:exon:3423;Parent=Msa1177520-mRNA-1
chr8_1 exon 9896106 9896286 9896106 ID=Msa1177520-mRNA-1:exon:3422;Parent=Msa1177520-mRNA-1
chr8_1 exon 9897052 9897408 9897052 ID=Msa1177520-mRNA-1:exon:3421;Parent=Msa1177520-mRNA-1
chr8_1 five_prime_UTR 9897278 9897408 9897278 ID=Msa1177520-mRNA-1:five_prime_utr;Parent=Msa1177520-mRNA-1
chr8_1 CDS 9897052 9897277 9897052 ID=Msa1177520-mRNA-1:cds;Parent=Msa1177520-mRNA-1
chr8_1 CDS 9896106 9896286 9896106 ID=Msa1177520-mRNA-1:cds;Parent=Msa1177520-mRNA-1
chr8_1 CDS 9895847 9895988 9895847 ID=Msa1177520-mRNA-1:cds;Parent=Msa1177520-mRNA-1
chr8_1 CDS 9895384 9895453 9895384 ID=Msa1177520-mRNA-1:cds;Parent=Msa1177520-mRNA-1
chr8_1 CDS 9895117 9895295 9895117 ID=Msa1177520-mRNA-1:cds;Parent=Msa1177520-mRNA-1
chr8_1 three_prime_UTR 9894764 9895116 9894764 ID=Msa1177520-mRNA-1:three_prime_utr;Parent=Msa1177520-mRNA-1
Gene Sequence

>Msa1177520

ATGGCTGACGAATCGGAGAAAAGCACCGTGACGGTGGATCGTGACGGCGGCGATTCGTTGCTTGAGAAGATCACCGAGAAGCTTCACTTTGAGGATGATTCTTCATCCGATTCTGATTCTCATTCTGATTTGGATAAGAAACCGGTTATTGAATCGGTGAAGGAGAAGGTTTTTCGCCTTTTTGGAAGGGAGAAGCCTGTTCACTCCGTCCTCGGCGGCGGAAAGCCTGCTGATGTGTTCTTGTGGAAGAACAAGAAGTTATCTGCTGGTACACTTGGGACAGCAACTGCTATTTGGGTGTTTTTTGAGCTGCTTGAGTATCATTTTCTCACTCTCATTTGTCACATTTCGATTCTGGTTCTTGCACTATTGTTTTTGTGGTCCAATGCTCATACCTTTGTTCACAAGACTCCACCTCACATTCCAATTGTTCATCTTCCTGAGGAACCATTTCTGCAGATTGCTTCTGCATTGAGAATTGAAATCAACCGAGGATTTTCTGCCTTGCGTGATATTGGTACTGGAAAAGATGTGAAGAAATTCCTCATTGTAATTTTTGGCTTGTGGATTCTGTCGATTGTTGGGAGCTGGAGCAATTTCTTAACCCTGTTCTACATATCTTTTGTTTTGTTGTTCACGGTGCCTTTCGTGTATGACAAGTACGAGGACAAGATAGACCCTCTGGCAGAAAAGGCATCCATCGAGATTAAAAAACAGTATGCTGTGTTTGACGAAAAGGTCTTGAGCAAGATCTTCAGCAAGATCCCAAAAGGTGTGTTGAAAGGAAAATTAGCTTAG
Protein sequence

>Msa1177520

MADESEKSTVTVDRDGGDSLLEKITEKLHFEDDSSSDSDSHSDLDKKPVIESVKEKVFRLFGREKPVHSVLGGGKPADVFLWKNKKLSAGTLGTATAIWVFFELLEYHFLTLICHISILVLALLFLWSNAHTFVHKTPPHIPIVHLPEEPFLQIASALRIEINRGFSALRDIGTGKDVKKFLIVIFGLWILSIVGSWSNFLTLFYISFVLLFTVPFVYDKYEDKIDPLAEKASIEIKKQYAVFDEKVLSKIFSKIPKGVLKGKLA*