AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1395660


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1395660 MtrunA17_Chr4g0007651 56.643 143 32 3 1 113 78 220 5.35e-36 122
Msa1395660 MtrunA17_Chr4g0007851 56.863 102 25 2 31 113 49 150 2.28e-24 90.5
Msa1395660 MtrunA17_Chr3g0139751 43.077 130 30 3 1 113 56 158 2.35e-23 88.2
Msa1395660 MtrunA17_Chr4g0014231 39.231 130 32 4 2 113 75 175 3.65e-18 75.1
Msa1395660 MtrunA17_Chr5g0405871 42.636 129 28 4 2 113 56 155 2.28e-17 72.8
Msa1395660 MtrunA17_Chr5g0405891 41.860 129 29 4 2 113 56 155 7.60e-17 71.2
Msa1395660 MtrunA17_Chr7g0221641 41.860 129 29 4 2 113 57 156 1.31e-16 70.9
Msa1395660 MtrunA17_Chr1g0202631 45.652 92 33 1 39 113 205 296 1.34e-16 73.2
Msa1395660 MtrunA17_Chr7g0259261 39.535 129 32 3 2 113 57 156 2.97e-16 69.7
Msa1395660 MtrunA17_Chr7g0259271 41.860 129 29 4 2 113 57 156 5.70e-16 69.3
Msa1395660 MtrunA17_Chr4g0014271 35.115 131 32 3 1 113 58 153 1.58e-15 67.8
Msa1395660 MtrunA17_Chr4g0015171 34.615 130 39 4 1 113 60 160 2.80e-15 67.4
Msa1395660 MtrunA17_Chr7g0259231 36.434 129 38 3 2 113 58 159 3.39e-15 67.0
Msa1395660 MtrunA17_Chr8g0361911 39.535 129 33 4 2 113 57 157 9.69e-15 65.9
Msa1395660 MtrunA17_Chr7g0259381 36.923 130 35 5 2 113 44 144 1.76e-14 65.1
Msa1395660 MtrunA17_Chr4g0014211 36.154 130 37 3 1 113 63 163 2.20e-14 65.1
Msa1395660 MtrunA17_Chr4g0014241 37.692 130 29 3 1 113 56 150 2.53e-14 64.7
Msa1395660 MtrunA17_Chr5g0438791 32.812 128 40 2 2 112 57 155 4.56e-14 64.3
Msa1395660 MtrunA17_Chr7g0259371 36.154 130 36 5 2 113 58 158 8.07e-14 63.5
Msa1395660 MtrunA17_Chr4g0014261 32.308 130 36 3 1 113 58 152 2.84e-13 62.0
Msa1395660 MtrunA17_Chr7g0221621 38.760 129 33 4 2 113 57 156 1.10e-12 60.5
Msa1395660 MtrunA17_Chr4g0015151 32.308 130 42 3 1 113 60 160 1.40e-12 60.5
Msa1395660 MtrunA17_Chr1g0167301 47.458 59 30 1 50 107 81 139 3.03e-12 61.2
Msa1395660 MtrunA17_Chr4g0009571 37.209 129 35 5 2 113 56 155 4.84e-12 58.9
Msa1395660 MtrunA17_Chr7g0259441 43.564 101 41 5 2 88 58 156 1.06e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1395660 AT4G34210.1 35.659 129 35 2 2 113 55 152 2.68e-17 72.8
Msa1395660 AT2G25700.1 36.434 129 43 3 2 113 57 163 6.07e-17 72.0
Msa1395660 AT1G20140.1 37.984 129 41 4 2 113 57 163 1.15e-16 71.6
Msa1395660 AT1G75950.1 38.760 129 39 3 2 113 55 160 1.89e-16 70.9
Msa1395660 AT4G34470.1 34.884 129 36 2 2 113 55 152 2.42e-16 70.5
Msa1395660 AT3G21850.1 33.077 130 39 3 1 113 55 153 1.22e-15 68.6
Msa1395660 AT5G42190.1 40.310 129 47 3 2 113 56 171 9.98e-15 66.6
Msa1395660 AT3G21860.1 33.077 130 39 2 1 113 54 152 3.87e-14 64.7
Msa1395660 AT3G21830.1 33.077 130 39 3 1 113 54 152 1.29e-12 60.8
Msa1395660 AT3G25650.1 34.091 132 50 5 1 113 54 167 1.76e-11 58.2

Find 24 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCGGGCTGCGGCTTATT+TGG 0.104601 tig0021957:+106544 Msa1395660:CDS
TACGTTGTTTGAACTCTTTC+GGG 0.185695 tig0021957:+106529 Msa1395660:CDS
TTACGTTGTTTGAACTCTTT+CGG 0.248726 tig0021957:+106528 Msa1395660:CDS
AGTGAGTAAGGAGGATGATC+TGG 0.299573 tig0021957:+106469 Msa1395660:CDS
TTTCGGGCTGCGGCTTATTT+GGG 0.306367 tig0021957:+106545 Msa1395660:CDS
ACTAAACTCCTCCTCTTCCT+TGG 0.354072 tig0021957:-106624 None:intergenic
TTTGGGTGTTGATAGTTTGC+TGG 0.372447 tig0021957:+106562 Msa1395660:CDS
CTAAACTCCTCCTCTTCCTT+GGG 0.433716 tig0021957:-106623 None:intergenic
CAAGTTTCAGCAGCAGCCTT+AGG 0.454196 tig0021957:-106434 None:intergenic
TGCAGAGAAAGAAGTGAGTA+AGG 0.460165 tig0021957:+106457 Msa1395660:CDS
GGAGGATGATCTGGAGAAGT+GGG 0.488161 tig0021957:+106478 Msa1395660:CDS
AGGAGGATGATCTGGAGAAG+TGG 0.503398 tig0021957:+106477 Msa1395660:CDS
GGAAGAGGAGGAGTTTAGTA+AGG 0.507410 tig0021957:+106628 Msa1395660:CDS
GTTTGAACTCTTTCGGGCTG+CGG 0.508842 tig0021957:+106535 Msa1395660:CDS
TTGCTGAAGAAGAAAAGCCT+TGG 0.514532 tig0021957:+106378 Msa1395660:CDS
TTCGAGAGATTTCTCTCCCA+AGG 0.518718 tig0021957:+106607 Msa1395660:CDS
AGATTTCTCTCCCAAGGAAG+AGG 0.524351 tig0021957:+106613 Msa1395660:CDS
AAGACCGATGAAAAGAAATC+CGG 0.542891 tig0021957:+106401 Msa1395660:CDS
ATCCGGTGAAGATGTTCCTA+AGG 0.572791 tig0021957:+106418 Msa1395660:CDS
GTTTGCTGGAGCTGACATGT+TGG 0.578098 tig0021957:+106576 Msa1395660:CDS
AGCCTTAGGAACATCTTCAC+CGG 0.585183 tig0021957:-106420 None:intergenic
AGAGAAAGAAGTGAGTAAGG+AGG 0.588345 tig0021957:+106460 Msa1395660:CDS
TTTCTCTCCCAAGGAAGAGG+AGG 0.597882 tig0021957:+106616 Msa1395660:CDS
AGTTTAGTAAGGAAAATCAA+TGG 0.605827 tig0021957:+106639 Msa1395660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGTTTAGTAAGGAAAATCAA+TGG + tig0021957:106639-106658 Msa1395660:CDS 25.0%
TTACGTTGTTTGAACTCTTT+CGG + tig0021957:106528-106547 Msa1395660:CDS 30.0%
AAGACCGATGAAAAGAAATC+CGG + tig0021957:106401-106420 Msa1395660:CDS 35.0%
! TTCACCGGATTTCTTTTCAT+CGG - tig0021957:106408-106427 None:intergenic 35.0%
TACGTTGTTTGAACTCTTTC+GGG + tig0021957:106529-106548 Msa1395660:CDS 35.0%
TTGCTGAAGAAGAAAAGCCT+TGG + tig0021957:106378-106397 Msa1395660:CDS 40.0%
! TTCTTTTCATCGGTCTTCCA+AGG - tig0021957:106398-106417 None:intergenic 40.0%
TGCAGAGAAAGAAGTGAGTA+AGG + tig0021957:106457-106476 Msa1395660:CDS 40.0%
AGAGAAAGAAGTGAGTAAGG+AGG + tig0021957:106460-106479 Msa1395660:CDS 40.0%
!! TTTGGGTGTTGATAGTTTGC+TGG + tig0021957:106562-106581 Msa1395660:CDS 40.0%
ATCCGGTGAAGATGTTCCTA+AGG + tig0021957:106418-106437 Msa1395660:CDS 45.0%
AGCCTTAGGAACATCTTCAC+CGG - tig0021957:106423-106442 None:intergenic 45.0%
AGTGAGTAAGGAGGATGATC+TGG + tig0021957:106469-106488 Msa1395660:CDS 45.0%
TTCGAGAGATTTCTCTCCCA+AGG + tig0021957:106607-106626 Msa1395660:CDS 45.0%
AGATTTCTCTCCCAAGGAAG+AGG + tig0021957:106613-106632 Msa1395660:CDS 45.0%
CTAAACTCCTCCTCTTCCTT+GGG - tig0021957:106626-106645 None:intergenic 45.0%
ACTAAACTCCTCCTCTTCCT+TGG - tig0021957:106627-106646 None:intergenic 45.0%
GGAAGAGGAGGAGTTTAGTA+AGG + tig0021957:106628-106647 Msa1395660:CDS 45.0%
CAAGTTTCAGCAGCAGCCTT+AGG - tig0021957:106437-106456 None:intergenic 50.0%
AGGAGGATGATCTGGAGAAG+TGG + tig0021957:106477-106496 Msa1395660:CDS 50.0%
GGAGGATGATCTGGAGAAGT+GGG + tig0021957:106478-106497 Msa1395660:CDS 50.0%
GTTTGAACTCTTTCGGGCTG+CGG + tig0021957:106535-106554 Msa1395660:CDS 50.0%
TTTCGGGCTGCGGCTTATTT+GGG + tig0021957:106545-106564 Msa1395660:CDS 50.0%
! GTTTGCTGGAGCTGACATGT+TGG + tig0021957:106576-106595 Msa1395660:CDS 50.0%
TTTCTCTCCCAAGGAAGAGG+AGG + tig0021957:106616-106635 Msa1395660:CDS 50.0%
CTTTCGGGCTGCGGCTTATT+TGG + tig0021957:106544-106563 Msa1395660:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0021957 gene 106332 106673 106332 ID=Msa1395660;Name=Msa1395660
tig0021957 mRNA 106332 106673 106332 ID=Msa1395660-mRNA-1;Parent=Msa1395660;Name=Msa1395660-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|-1|0|1|-1|1|1|0|113
tig0021957 exon 106332 106673 106332 ID=Msa1395660-mRNA-1:exon:14976;Parent=Msa1395660-mRNA-1
tig0021957 CDS 106332 106673 106332 ID=Msa1395660-mRNA-1:cds;Parent=Msa1395660-mRNA-1
Gene Sequence

>Msa1395660

ATGGTAATTGATTATTGCAAGAAACATCATGTTGAAGCTGCTGCGTTTGCTGAAGAAGAAAAGCCTTGGAAGACCGATGAAAAGAAATCCGGTGAAGATGTTCCTAAGGCTGCTGCTGAAACTTGTGCAGAGAAAGAAGTGAGTAAGGAGGATGATCTGGAGAAGTGGGATGCTGAATTTGTTAATGTTGATCATGTTACGTTGTTTGAACTCTTTCGGGCTGCGGCTTATTTGGGTGTTGATAGTTTGCTGGAGCTGACATGTTGGACTGTGACTTCGAGAGATTTCTCTCCCAAGGAAGAGGAGGAGTTTAGTAAGGAAAATCAATGGCATTTTGAATGA

Protein sequence

>Msa1395660

MVIDYCKKHHVEAAAFAEEEKPWKTDEKKSGEDVPKAAAETCAEKEVSKEDDLEKWDAEFVNVDHVTLFELFRAAAYLGVDSLLELTCWTVTSRDFSPKEEEEFSKENQWHFE*