AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0320120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0320120 MtrunA17_Chr6g0469071 67.164 67 11 2 97 153 1 66 1.43e-17 79.0
Msa0320120 MtrunA17_Chr1g0153901 65.672 67 12 2 97 153 1 66 2.67e-17 77.8
Msa0320120 MtrunA17_Chr7g0261491 89.655 29 3 0 69 97 342 370 8.22e-13 65.1
Msa0320120 MtrunA17_Chr1g0183651 86.207 29 4 0 69 97 342 370 1.18e-12 64.7
Msa0320120 MtrunA17_Chr3g0135951 82.759 29 5 0 69 97 325 353 3.65e-12 63.2
Msa0320120 MtrunA17_Chr2g0299781 82.759 29 5 0 69 97 344 372 5.59e-12 62.8
Msa0320120 MtrunA17_Chr1g0167351 82.759 29 5 0 69 97 334 362 1.09e-11 62.0
Msa0320120 MtrunA17_Chr1g0187581 82.759 29 5 0 69 97 340 368 3.55e-11 60.5
Msa0320120 MtrunA17_Chr1g0167311 76.667 30 7 0 69 98 339 368 7.13e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0320120 AT1G03920.1 43.529 85 37 3 69 150 359 435 5.09e-13 66.2
Msa0320120 AT1G03920.3 43.529 85 37 3 69 150 359 435 5.09e-13 66.2
Msa0320120 AT2G20470.3 89.655 29 3 0 69 97 345 373 6.14e-13 65.9
Msa0320120 AT2G20470.2 89.655 29 3 0 69 97 345 373 6.73e-13 65.9
Msa0320120 AT2G20470.1 89.655 29 3 0 69 97 345 373 6.73e-13 65.9
Msa0320120 AT1G03920.2 43.529 85 37 3 69 150 246 322 8.82e-13 65.5
Msa0320120 AT2G19400.1 82.759 29 5 0 69 97 336 364 4.39e-12 63.5
Msa0320120 AT4G33080.2 82.759 29 5 0 69 97 325 353 4.44e-12 63.2
Msa0320120 AT4G14350.3 82.759 29 5 0 69 97 342 370 5.12e-12 63.2
Msa0320120 AT4G14350.2 82.759 29 5 0 69 97 342 370 5.12e-12 63.2
Msa0320120 AT4G14350.1 82.759 29 5 0 69 97 342 370 5.12e-12 63.2
Msa0320120 AT4G14350.4 82.759 29 5 0 69 97 342 370 5.12e-12 63.2
Msa0320120 AT4G33080.1 82.759 29 5 0 69 97 325 353 5.56e-12 63.2
Msa0320120 AT3G23310.1 70.588 34 10 0 69 102 345 378 3.87e-11 60.5

Find 65 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAAATATCAGTGAATTTA+AGG 0.109116 2_4:-20394501 Msa0320120:intron
TCAAAGAAAGCAATTGAATA+TGG 0.281933 2_4:-20393863 Msa0320120:three_prime_UTR
ACAGATCTGAGGTAAATTTC+TGG 0.291373 2_4:+20396872 None:intergenic
TTGTGTATTAATCATAAATA+TGG 0.331416 2_4:-20393609 Msa0320120:three_prime_UTR
GAGAGTGCTTCACATGCTTC+AGG 0.340669 2_4:+20393909 None:intergenic
CTCACCTGATTTCAGAACTT+TGG 0.343040 2_4:+20394987 None:intergenic
ATTGGATCATCAGAATAAAA+AGG 0.356530 2_4:+20396113 None:intergenic
GAGTCTAATGACTTAAGTTT+TGG 0.358699 2_4:-20393948 Msa0320120:three_prime_UTR
CCATTCTTACTCGACGTTGC+TGG 0.367976 2_4:-20396724 Msa0320120:CDS
CATATACGTTCTTTAAGAAA+AGG 0.395907 2_4:+20394542 None:intergenic
GTAGAAACTGGGAGGTGTTA+TGG 0.399971 2_4:-20394941 Msa0320120:intron
ACTATCACTAACAACATTAT+CGG 0.417376 2_4:+20393771 None:intergenic
AAGAATCTGATTATTGTTGA+TGG 0.422920 2_4:-20393721 Msa0320120:three_prime_UTR
ACCACCAAAGTTCTGAAATC+AGG 0.425415 2_4:-20394991 Msa0320120:intron
ACGTATATGGAAGAGGTTCT+TGG 0.432015 2_4:-20394528 Msa0320120:three_prime_UTR
GGAGGATCGTGTTCCGAGTT+GGG 0.451029 2_4:+20394578 None:intergenic
TTTCATCGTAGTGGCACCTC+TGG 0.455751 2_4:+20395020 None:intergenic
GATAGTGACTTAGATGTTGT+TGG 0.458086 2_4:-20393754 Msa0320120:three_prime_UTR
CGGAGGATCGTGTTCCGAGT+TGG 0.471300 2_4:+20394577 None:intergenic
GCATGTGAAGCACTCTCAAA+AGG 0.473608 2_4:-20393903 Msa0320120:three_prime_UTR
CTTAGATGTTGTTGGTCTTG+AGG 0.474267 2_4:-20393746 Msa0320120:three_prime_UTR
AGAAAAGGATACATAATATC+CGG 0.480833 2_4:+20394557 None:intergenic
ACCTGATTTCAGAACTTTGG+TGG 0.481782 2_4:+20394990 None:intergenic
ATTATTGTTGATGGAACCAT+TGG 0.482478 2_4:-20393712 Msa0320120:three_prime_UTR
TAAATTCACAGGTGGTCACT+TGG 0.489963 2_4:-20396170 Msa0320120:intron
TTAAAGAACGTATATGGAAG+AGG 0.490513 2_4:-20394535 Msa0320120:three_prime_UTR
TTAAGTTCACTAGACGAAGA+AGG 0.495636 2_4:+20395054 None:intergenic
ATTGAATATGGAAGAAAATG+TGG 0.500975 2_4:-20393851 Msa0320120:three_prime_UTR
TGCTGCATAACTCTTAAACT+TGG 0.507255 2_4:+20394907 None:intergenic
GCTTGAGGTTTGTCCTAGAT+CGG 0.507765 2_4:-20393809 Msa0320120:three_prime_UTR
CCAGCAACGTCGAGTAAGAA+TGG 0.508399 2_4:+20396724 None:intergenic
TAAGTTCACTAGACGAAGAA+GGG 0.513899 2_4:+20395055 None:intergenic
TGAAGTTGGAAGTGAACCCT+TGG 0.523892 2_4:-20393670 Msa0320120:three_prime_UTR
ACCTTCCTACATGTTGACAT+TGG 0.524308 2_4:+20396095 None:intergenic
CATGTATATGTCCATGTGGT+TGG 0.525817 2_4:-20393529 Msa0320120:three_prime_UTR
CATATTAATATCCTAAAACA+TGG 0.554096 2_4:+20393633 None:intergenic
ATATGACGAATTTGAGCTTG+AGG 0.567465 2_4:-20393824 Msa0320120:three_prime_UTR
TGGACTCAAAAGTTCTGCCA+AGG 0.573018 2_4:+20393653 None:intergenic
CAACTCGGAACACGATCCTC+CGG 0.576955 2_4:-20394576 Msa0320120:three_prime_UTR
AGCTATTGTGCATGAAATGT+TGG 0.577959 2_4:-20396147 Msa0320120:CDS
ATGATCCAATGTCAACATGT+AGG 0.578246 2_4:-20396100 Msa0320120:CDS
ACTAGACGAAGAAGGGATGT+TGG 0.591690 2_4:+20395062 None:intergenic
GCGGCAAAATGTTCAGCCAA+TGG 0.593066 2_4:+20393696 None:intergenic
GGAGAGTGAACTCACGGCGG+AGG 0.596524 2_4:+20396793 None:intergenic
TCCAATGTCAACATGTAGGA+AGG 0.597352 2_4:-20396096 Msa0320120:intron
GAGAGTAAACTAAACTGTAC+CGG 0.600791 2_4:+20396749 None:intergenic
TTCACTTCCAACTTCAAATG+CGG 0.601113 2_4:+20393677 None:intergenic
GCGGAGGTTACGCGAACTGA+CGG 0.612584 2_4:+20396809 None:intergenic
CAGTTTCATCTCGACACCGC+CGG 0.615730 2_4:-20396768 Msa0320120:CDS
TCACTAACAACATTATCGGA+AGG 0.627876 2_4:+20393775 None:intergenic
TATTGTGCATGAAATGTTGG+TGG 0.631431 2_4:-20396144 Msa0320120:CDS
CATCCTCAAAACCAACCACA+TGG 0.632853 2_4:+20393518 None:intergenic
TTATATTACACATCCCAACT+CGG 0.644448 2_4:-20394591 Msa0320120:three_prime_UTR
CGGTGTCGAGATGAAACTGT+CGG 0.657508 2_4:+20396772 None:intergenic
ACTGTCGGAGAGTGAACTCA+CGG 0.661336 2_4:+20396787 None:intergenic
GGACTCAAAAGTTCTGCCAA+GGG 0.664088 2_4:+20393654 None:intergenic
TCAACTTTAATGTAGAAACT+GGG 0.666117 2_4:-20394952 Msa0320120:intron
TTATCATGTATATGTCCATG+TGG 0.667951 2_4:-20393533 Msa0320120:three_prime_UTR
AAAGGATACATAATATCCGG+AGG 0.676498 2_4:+20394560 None:intergenic
ATTGTGCATGAAATGTTGGT+GGG 0.689487 2_4:-20396143 Msa0320120:CDS
ACTTTAATGTAGAAACTGGG+AGG 0.739092 2_4:-20394949 Msa0320120:intron
AGTAAACTAAACTGTACCGG+CGG 0.741081 2_4:+20396752 None:intergenic
GCAACGTCGAGTAAGAATGG+CGG 0.760998 2_4:+20396727 None:intergenic
GACGGCGTCGAAAAGAAACG+CGG 0.775873 2_4:+20396692 None:intergenic
GTCGGAGAGTGAACTCACGG+CGG 0.800954 2_4:+20396790 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAAAATAGAAAATTATTAA+TGG - chr2_4:20393905-20393924 Msa0320120:three_prime_UTR 10.0%
!! CAAAATAGAAAATTATTAAT+GGG - chr2_4:20393906-20393925 Msa0320120:three_prime_UTR 10.0%
!! ATTATATTAGATAAACAAAA+TGG + chr2_4:20394525-20394544 None:intergenic 10.0%
!!! ACATGTTTTAAAATTAAAAA+AGG - chr2_4:20394980-20394999 Msa0320120:intron 10.0%
!! TTTATCATATAATAAATCAT+GGG + chr2_4:20396235-20396254 None:intergenic 10.0%
!!! TTTTATCATATAATAAATCA+TGG + chr2_4:20396236-20396255 None:intergenic 10.0%
!!! TTCTTCAAATTATTGTTTTA+GGG - chr2_4:20393880-20393899 Msa0320120:three_prime_UTR 15.0%
!! TAATCATTCACTATAAAAAT+AGG - chr2_4:20393962-20393981 Msa0320120:intron 15.0%
!! AATCAAATAAATTTAACATG+TGG + chr2_4:20395139-20395158 None:intergenic 15.0%
!! TCTGTTTAACAAAAAAAAAA+AGG + chr2_4:20395299-20395318 None:intergenic 15.0%
!! CAATAGACATAATAATTAAA+TGG + chr2_4:20395637-20395656 None:intergenic 15.0%
!! TTGTGTATTAATCATAAATA+TGG - chr2_4:20396788-20396807 Msa0320120:CDS 15.0%
!!! TTTTTATATGGTTAGTTTTT+AGG - chr2_4:20396819-20396838 Msa0320120:CDS 15.0%
!!! AATTTATCTGACTTGTTAAT+TGG - chr2_4:20393803-20393822 Msa0320120:three_prime_UTR 20.0%
!!! GTTCTTCAAATTATTGTTTT+AGG - chr2_4:20393879-20393898 Msa0320120:three_prime_UTR 20.0%
!!! TACTTTATTGTTGTTTAATG+TGG - chr2_4:20394131-20394150 Msa0320120:intron 20.0%
!! GTAAATACACAAGAATAATT+AGG - chr2_4:20394677-20394696 Msa0320120:three_prime_UTR 20.0%
!! TAATTAAGTGTCATAGTAAT+CGG - chr2_4:20395106-20395125 Msa0320120:intron 20.0%
!! CAATTAAGTTAATATACACA+AGG + chr2_4:20395176-20395195 None:intergenic 20.0%
!!! TTTTTTGTTAAACAGATAGA+TGG - chr2_4:20395303-20395322 Msa0320120:intron 20.0%
!!! TTTTTGTTAAACAGATAGAT+GGG - chr2_4:20395304-20395323 Msa0320120:intron 20.0%
!! TAGTGAACTTAAAAAAAGAA+AGG - chr2_4:20395354-20395373 Msa0320120:intron 20.0%
!! TTATTATGTCTATTGATCTT+AGG - chr2_4:20395642-20395661 Msa0320120:intron 20.0%
!! AGAAAGTAATGATCTAAAAA+AGG - chr2_4:20395711-20395730 Msa0320120:intron 20.0%
!! TCAAAATATCAGTGAATTTA+AGG - chr2_4:20395896-20395915 Msa0320120:intron 20.0%
!!! TTTTCAGTAGAATTTGAATT+GGG + chr2_4:20396174-20396193 None:intergenic 20.0%
!!! TTTTTCAGTAGAATTTGAAT+TGG + chr2_4:20396175-20396194 None:intergenic 20.0%
!!! CATGAAAAAACAAACATTTT+TGG + chr2_4:20396200-20396219 None:intergenic 20.0%
!! CATATTAATATCCTAAAACA+TGG + chr2_4:20396767-20396786 None:intergenic 20.0%
! AACAATAATTTGAAGAACGA+AGG + chr2_4:20393877-20393896 None:intergenic 25.0%
!!! TTAATGGGTTGAAATTGAAT+AGG - chr2_4:20393921-20393940 Msa0320120:three_prime_UTR 25.0%
! TTGAAATTGAATAGGATTTG+TGG - chr2_4:20393929-20393948 Msa0320120:three_prime_UTR 25.0%
! TGAAATTGAATAGGATTTGT+GGG - chr2_4:20393930-20393949 Msa0320120:three_prime_UTR 25.0%
! AGGAAAAAAGAGAAAAACTA+AGG - chr2_4:20394005-20394024 Msa0320120:intron 25.0%
! GGAAAAAAGAGAAAAACTAA+GGG - chr2_4:20394006-20394025 Msa0320120:intron 25.0%
! CTATGATAAATCATGTCTAA+TGG + chr2_4:20394069-20394088 None:intergenic 25.0%
! CATTAGACATGATTTATCAT+AGG - chr2_4:20394067-20394086 Msa0320120:intron 25.0%
!! CAATAAAGTATTTCATCACT+AGG + chr2_4:20394121-20394140 None:intergenic 25.0%
! AATAAAACTTTCTTGAGATC+AGG - chr2_4:20394162-20394181 Msa0320120:intron 25.0%
! ATAAAACTTTCTTGAGATCA+GGG - chr2_4:20394163-20394182 Msa0320120:intron 25.0%
!! ACAGTTGTTATTAAATTCAC+AGG - chr2_4:20394216-20394235 Msa0320120:intron 25.0%
! ATTGGATCATCAGAATAAAA+AGG + chr2_4:20394287-20394306 None:intergenic 25.0%
! AAAAATGTTTGCTTCACATA+AGG - chr2_4:20394337-20394356 Msa0320120:intron 25.0%
! GTTTGAAGAAACAAAAAAGA+AGG + chr2_4:20394378-20394397 None:intergenic 25.0%
!!! GGACTAAATGCTATTTTTAA+GGG + chr2_4:20394412-20394431 None:intergenic 25.0%
!!! AATATATTTTTTGTGACCTG+TGG - chr2_4:20394588-20394607 Msa0320120:three_prime_UTR 25.0%
!! AACCTTTGATTTAGTATTCT+TGG - chr2_4:20394817-20394836 Msa0320120:intron 25.0%
! TACCAAGAATACTAAATCAA+AGG + chr2_4:20394822-20394841 None:intergenic 25.0%
!! TTAATAAGTTTAGTTCCCAA+GGG + chr2_4:20394863-20394882 None:intergenic 25.0%
!! ATTAATAAGTTTAGTTCCCA+AGG + chr2_4:20394864-20394883 None:intergenic 25.0%
!! AATACTCGATAGTTTTCATA+CGG + chr2_4:20395247-20395266 None:intergenic 25.0%
! AATCAGGTGAGAAAATAAAA+AGG - chr2_4:20395422-20395441 Msa0320120:intron 25.0%
! TCAACTTTAATGTAGAAACT+GGG - chr2_4:20395445-20395464 Msa0320120:intron 25.0%
! AAAACACAAAAGATGCAATT+TGG - chr2_4:20395568-20395587 Msa0320120:intron 25.0%
! AAACACAAAAGATGCAATTT+GGG - chr2_4:20395569-20395588 Msa0320120:intron 25.0%
!!! AAAAAATGGAAGGTGTTTTT+GGG - chr2_4:20395745-20395764 Msa0320120:intron 25.0%
! AGAAAAGGATACATAATATC+CGG + chr2_4:20395843-20395862 None:intergenic 25.0%
!! CATATACGTTCTTTAAGAAA+AGG + chr2_4:20395858-20395877 None:intergenic 25.0%
!! CTTTTCTTAAAGAACGTATA+TGG - chr2_4:20395856-20395875 Msa0320120:intron 25.0%
! AGACAAAAAGTCAAAAAAGT+AGG - chr2_4:20396006-20396025 Msa0320120:intron 25.0%
! ATTCAAAGTTAACATCACAA+CGG + chr2_4:20396125-20396144 None:intergenic 25.0%
! TCAAAGAAAGCAATTGAATA+TGG - chr2_4:20396534-20396553 Msa0320120:intron 25.0%
! ATTGAATATGGAAGAAAATG+TGG - chr2_4:20396546-20396565 Msa0320120:intron 25.0%
! ACTATCACTAACAACATTAT+CGG + chr2_4:20396629-20396648 None:intergenic 25.0%
! AAGAATCTGATTATTGTTGA+TGG - chr2_4:20396676-20396695 Msa0320120:intron 25.0%
!!! ATGGCTATGTACTTTTTATA+TGG - chr2_4:20396807-20396826 Msa0320120:CDS 25.0%
! TAAAGTATTTCATCACTAGG+TGG + chr2_4:20394118-20394137 None:intergenic 30.0%
! GTTGTTATTAAATTCACAGG+TGG - chr2_4:20394219-20394238 Msa0320120:intron 30.0%
!!! GGGACTAAATGCTATTTTTA+AGG + chr2_4:20394413-20394432 None:intergenic 30.0%
TGTACAACTTGTATAGTTCA+GGG + chr2_4:20394495-20394514 None:intergenic 30.0%
TCCAAGCAGAAACTAAAAAT+TGG + chr2_4:20394720-20394739 None:intergenic 30.0%
! TTCCTTATTAGTCTCTATGA+AGG - chr2_4:20394780-20394799 Msa0320120:intron 30.0%
TTCCTTCATAGAGACTAATA+AGG + chr2_4:20394785-20394804 None:intergenic 30.0%
TCTCTCATATATCTTCTTCA+TGG + chr2_4:20394889-20394908 None:intergenic 30.0%
GAAGAAGATATATGAGAGAA+CGG - chr2_4:20394890-20394909 Msa0320120:intron 30.0%
AAGAAGATATATGAGAGAAC+GGG - chr2_4:20394891-20394910 Msa0320120:intron 30.0%
ATCATGACCATCTATTAAAG+AGG - chr2_4:20394950-20394969 Msa0320120:intron 30.0%
TATCTCACCTCTTTAATAGA+TGG + chr2_4:20394960-20394979 None:intergenic 30.0%
! ATTTATTGACTGCAGAAAAC+AGG - chr2_4:20395027-20395046 Msa0320120:CDS 30.0%
!!! GGTAATTTTTTTCATCGTAG+TGG + chr2_4:20395389-20395408 None:intergenic 30.0%
GTCAACTTTAATGTAGAAAC+TGG - chr2_4:20395444-20395463 Msa0320120:intron 30.0%
! TTTGTGTTTTCATCTACCAT+TGG + chr2_4:20395558-20395577 None:intergenic 30.0%
AGGATCCATTGAAGAAAAAA+TGG - chr2_4:20395731-20395750 Msa0320120:intron 30.0%
TCCATTGAAGAAAAAATGGA+AGG - chr2_4:20395735-20395754 Msa0320120:intron 30.0%
!!! ACCTTCCATTTTTTCTTCAA+TGG + chr2_4:20395739-20395758 None:intergenic 30.0%
!!! GAAAAAATGGAAGGTGTTTT+TGG - chr2_4:20395744-20395763 Msa0320120:intron 30.0%
!!! AAAAATGGAAGGTGTTTTTG+GGG - chr2_4:20395746-20395765 Msa0320120:intron 30.0%
TTATATTACACATCCCAACT+CGG - chr2_4:20395806-20395825 Msa0320120:intron 30.0%
TTAAAGAACGTATATGGAAG+AGG - chr2_4:20395862-20395881 Msa0320120:intron 30.0%
! CAATTCTGGAAGAATTAAGA+GGG + chr2_4:20396069-20396088 None:intergenic 30.0%
TTCTTCCAGAATTGCTAAAA+TGG - chr2_4:20396075-20396094 Msa0320120:intron 30.0%
TCTTCCAGAATTGCTAAAAT+GGG - chr2_4:20396076-20396095 Msa0320120:intron 30.0%
ATTCGTCGAAATTGTGTTTA+TGG - chr2_4:20396351-20396370 Msa0320120:intron 30.0%
CCATATTTACAAATAGCAAC+AGG + chr2_4:20396389-20396408 None:intergenic 30.0%
!! CCTGTTGCTATTTGTAAATA+TGG - chr2_4:20396386-20396405 Msa0320120:intron 30.0%
TTACTTCTCTGTCTTATGTT+TGG - chr2_4:20396414-20396433 Msa0320120:intron 30.0%
! GAGTCTAATGACTTAAGTTT+TGG - chr2_4:20396449-20396468 Msa0320120:intron 30.0%
! ATTATTGTTGATGGAACCAT+TGG - chr2_4:20396685-20396704 Msa0320120:intron 30.0%
!! AACTTTTGAGTCCATGTTTT+AGG - chr2_4:20396753-20396772 Msa0320120:CDS 30.0%
TTATCATGTATATGTCCATG+TGG - chr2_4:20396864-20396883 Msa0320120:CDS 30.0%
! ACAGATCTGAGGTAAATTTC+TGG + chr2_4:20393528-20393547 None:intergenic 35.0%
!! TTTTAGGTGAAACAGATCTG+AGG + chr2_4:20393539-20393558 None:intergenic 35.0%
!!! GAGATGCGACACAATTTTTT+AGG + chr2_4:20393555-20393574 None:intergenic 35.0%
GAGAGTAAACTAAACTGTAC+CGG + chr2_4:20393651-20393670 None:intergenic 35.0%
TCACAGTTTCTATTGTTCTG+AGG - chr2_4:20393768-20393787 Msa0320120:three_prime_UTR 35.0%
TTGAAGAACGAAGGAGAAAT+TGG + chr2_4:20393868-20393887 None:intergenic 35.0%
! AATAGGAACAAGTTGACTAG+AGG - chr2_4:20393979-20393998 Msa0320120:intron 35.0%
AACTAAGGGTCGAACAATTA+AGG - chr2_4:20394020-20394039 Msa0320120:intron 35.0%
AGCTATTGTGCATGAAATGT+TGG - chr2_4:20394250-20394269 Msa0320120:intron 35.0%
TATTGTGCATGAAATGTTGG+TGG - chr2_4:20394253-20394272 Msa0320120:intron 35.0%
ATTGTGCATGAAATGTTGGT+GGG - chr2_4:20394254-20394273 Msa0320120:intron 35.0%
GGATCATCAGAATAAAAAGG+TGG + chr2_4:20394284-20394303 None:intergenic 35.0%
ATGATCCAATGTCAACATGT+AGG - chr2_4:20394297-20394316 Msa0320120:intron 35.0%
CAAGTGAGTGAAAGTTTATG+GGG + chr2_4:20394433-20394452 None:intergenic 35.0%
GCAAGTGAGTGAAAGTTTAT+GGG + chr2_4:20394434-20394453 None:intergenic 35.0%
TGCAAGTGAGTGAAAGTTTA+TGG + chr2_4:20394435-20394454 None:intergenic 35.0%
GTACAACTTGTATAGTTCAG+GGG + chr2_4:20394494-20394513 None:intergenic 35.0%
GTGTACAACTTGTATAGTTC+AGG + chr2_4:20394496-20394515 None:intergenic 35.0%
TAACAAATTCATCATGCCAC+AGG + chr2_4:20394607-20394626 None:intergenic 35.0%
!! TGGCATGATGAATTTGTTAC+TGG - chr2_4:20394608-20394627 Msa0320120:three_prime_UTR 35.0%
TGGGACACTCAAATTATTGT+TGG - chr2_4:20394655-20394674 Msa0320120:three_prime_UTR 35.0%
!!! GCCAATTTTTAGTTTCTGCT+TGG - chr2_4:20394716-20394735 Msa0320120:intron 35.0%
ACTAATAAGGAACATCCCTT+AGG + chr2_4:20394772-20394791 None:intergenic 35.0%
ACACATGAGAAAAATGTCAC+TGG + chr2_4:20394929-20394948 None:intergenic 35.0%
! AGGAGAGAGAGTGTAATTAA+TGG - chr2_4:20395000-20395019 Msa0320120:CDS 35.0%
! TTATTAGTTCTCTCAGGGAA+AGG + chr2_4:20395075-20395094 None:intergenic 35.0%
TTTCCCTGAGAGAACTAATA+AGG - chr2_4:20395074-20395093 Msa0320120:CDS 35.0%
! CAACCTTATTAGTTCTCTCA+GGG + chr2_4:20395080-20395099 None:intergenic 35.0%
TAAGTTCACTAGACGAAGAA+GGG + chr2_4:20395345-20395364 None:intergenic 35.0%
TTAAGTTCACTAGACGAAGA+AGG + chr2_4:20395346-20395365 None:intergenic 35.0%
CTTAAAAAAAGAAAGGCCAG+AGG - chr2_4:20395361-20395380 Msa0320120:intron 35.0%
ACTTTAATGTAGAAACTGGG+AGG - chr2_4:20395448-20395467 Msa0320120:intron 35.0%
TGCTGCATAACTCTTAAACT+TGG + chr2_4:20395493-20395512 None:intergenic 35.0%
AAAGGATACATAATATCCGG+AGG + chr2_4:20395840-20395859 None:intergenic 35.0%
! GCAATTCTGGAAGAATTAAG+AGG + chr2_4:20396070-20396089 None:intergenic 35.0%
! TTGATTGATTCTGATAGTGC+TGG - chr2_4:20396264-20396283 Msa0320120:intron 35.0%
AATGATGTTGAAGTGACTGA+TGG - chr2_4:20396294-20396313 Msa0320120:intron 35.0%
! AAGCACTCTCAAAAGGTTTT+TGG - chr2_4:20396501-20396520 Msa0320120:intron 35.0%
ATATGACGAATTTGAGCTTG+AGG - chr2_4:20396573-20396592 Msa0320120:intron 35.0%
GGAAAAAGTAAAACCGATCT+AGG + chr2_4:20396604-20396623 None:intergenic 35.0%
TCACTAACAACATTATCGGA+AGG + chr2_4:20396625-20396644 None:intergenic 35.0%
GATAGTGACTTAGATGTTGT+TGG - chr2_4:20396643-20396662 Msa0320120:intron 35.0%
TTCACTTCCAACTTCAAATG+CGG + chr2_4:20396723-20396742 None:intergenic 35.0%
AGTAAACTAAACTGTACCGG+CGG + chr2_4:20393648-20393667 None:intergenic 40.0%
AGAAACTGTGAGTGAGAACA+GGG + chr2_4:20393759-20393778 None:intergenic 40.0%
TAGAAACTGTGAGTGAGAAC+AGG + chr2_4:20393760-20393779 None:intergenic 40.0%
! AACAAGTTGACTAGAGGTTG+AGG - chr2_4:20393985-20394004 Msa0320120:intron 40.0%
TAAATTCACAGGTGGTCACT+TGG - chr2_4:20394227-20394246 Msa0320120:intron 40.0%
TCCAATGTCAACATGTAGGA+AGG - chr2_4:20394301-20394320 Msa0320120:intron 40.0%
ACCTTCCTACATGTTGACAT+TGG + chr2_4:20394305-20394324 None:intergenic 40.0%
AGTTCAGGGGCTAAATCAAA+AGG + chr2_4:20394481-20394500 None:intergenic 40.0%
GTTGAAATCGTAGTATGTGC+TGG - chr2_4:20394630-20394649 Msa0320120:three_prime_UTR 40.0%
!! TTGGTAGTGATGTTCCTTCA+TGG - chr2_4:20394836-20394855 Msa0320120:intron 40.0%
AAGATATATGAGAGAACGGG+AGG - chr2_4:20394894-20394913 Msa0320120:CDS 40.0%
AGATATATGAGAGAACGGGA+GGG - chr2_4:20394895-20394914 Msa0320120:CDS 40.0%
AAAACAGGAGAGTTTCTCAC+TGG - chr2_4:20395042-20395061 Msa0320120:CDS 40.0%
! GCAACCTTATTAGTTCTCTC+AGG + chr2_4:20395081-20395100 None:intergenic 40.0%
! ACTCGATAGTTTTCATACGG+TGG + chr2_4:20395244-20395263 None:intergenic 40.0%
!! ACCACCAAAGTTCTGAAATC+AGG - chr2_4:20395406-20395425 Msa0320120:intron 40.0%
ACCTGATTTCAGAACTTTGG+TGG + chr2_4:20395410-20395429 None:intergenic 40.0%
CTCACCTGATTTCAGAACTT+TGG + chr2_4:20395413-20395432 None:intergenic 40.0%
TAGAGTAGATGAGTAGCCAA+TGG - chr2_4:20395539-20395558 Msa0320120:intron 40.0%
ACGTATATGGAAGAGGTTCT+TGG - chr2_4:20395869-20395888 Msa0320120:intron 40.0%
! TTCTGGAAGAATTAAGAGGG+AGG + chr2_4:20396066-20396085 None:intergenic 40.0%
!! GAGACCCATTTTAGCAATTC+TGG + chr2_4:20396083-20396102 None:intergenic 40.0%
CAAAGTTAACATCACAACGG+TGG + chr2_4:20396122-20396141 None:intergenic 40.0%
TGAAGTGACTGATGGTATAG+TGG - chr2_4:20396302-20396321 Msa0320120:intron 40.0%
!! CTTAGATGTTGTTGGTCTTG+AGG - chr2_4:20396651-20396670 Msa0320120:intron 40.0%
!! CATTTTGCCGCATTTGAAGT+TGG - chr2_4:20396713-20396732 Msa0320120:CDS 40.0%
CATGTATATGTCCATGTGGT+TGG - chr2_4:20396868-20396887 Msa0320120:CDS 40.0%
ACTTGCGTAGTTTGAACCGA+CGG + chr2_4:20393726-20393745 None:intergenic 45.0%
GAAACTGTGAGTGAGAACAG+GGG + chr2_4:20393758-20393777 None:intergenic 45.0%
! AATCGTAGTATGTGCTGGCA+TGG - chr2_4:20394635-20394654 Msa0320120:three_prime_UTR 45.0%
! ATCGTAGTATGTGCTGGCAT+GGG - chr2_4:20394636-20394655 Msa0320120:three_prime_UTR 45.0%
CATGGACACTCTTCCTTCTA+CGG + chr2_4:20394743-20394762 None:intergenic 45.0%
!! AGAAGGAAGAGTGTCCATGA+AGG - chr2_4:20394744-20394763 Msa0320120:intron 45.0%
AGTGTCCATGAAGGAACCTA+AGG - chr2_4:20394753-20394772 Msa0320120:intron 45.0%
GTGTCCATGAAGGAACCTAA+GGG - chr2_4:20394754-20394773 Msa0320120:intron 45.0%
ACATCCCTTAGGTTCCTTCA+TGG + chr2_4:20394761-20394780 None:intergenic 45.0%
ATGTTCCTTCATGGTCCCTT+GGG - chr2_4:20394845-20394864 Msa0320120:intron 45.0%
GATATATGAGAGAACGGGAG+GGG - chr2_4:20394896-20394915 Msa0320120:CDS 45.0%
ACTAGACGAAGAAGGGATGT+TGG + chr2_4:20395338-20395357 None:intergenic 45.0%
!! GTAGAAACTGGGAGGTGTTA+TGG - chr2_4:20395456-20395475 Msa0320120:intron 45.0%
! GCATGTGAAGCACTCTCAAA+AGG - chr2_4:20396494-20396513 Msa0320120:intron 45.0%
GCTTGAGGTTTGTCCTAGAT+CGG - chr2_4:20396588-20396607 Msa0320120:intron 45.0%
TGAAGTTGGAAGTGAACCCT+TGG - chr2_4:20396727-20396746 Msa0320120:CDS 45.0%
GGACTCAAAAGTTCTGCCAA+GGG + chr2_4:20396746-20396765 None:intergenic 45.0%
TGGACTCAAAAGTTCTGCCA+AGG + chr2_4:20396747-20396766 None:intergenic 45.0%
ACTGTCGGAGAGTGAACTCA+CGG + chr2_4:20393613-20393632 None:intergenic 50.0%
! CGGTGTCGAGATGAAACTGT+CGG + chr2_4:20393628-20393647 None:intergenic 50.0%
GCAACGTCGAGTAAGAATGG+CGG + chr2_4:20393673-20393692 None:intergenic 50.0%
CCAGCAACGTCGAGTAAGAA+TGG + chr2_4:20393676-20393695 None:intergenic 50.0%
CCATTCTTACTCGACGTTGC+TGG - chr2_4:20393673-20393692 Msa0320120:three_prime_UTR 50.0%
GTTTCTGCTTGGACCGTAGA+AGG - chr2_4:20394727-20394746 Msa0320120:intron 50.0%
GATGTTCCTTCATGGTCCCT+TGG - chr2_4:20394844-20394863 Msa0320120:intron 50.0%
TAGTTCCCAAGGGACCATGA+AGG + chr2_4:20394853-20394872 None:intergenic 50.0%
AGGAGAGTTTCTCACTGGAG+AGG - chr2_4:20395047-20395066 Msa0320120:CDS 50.0%
TTTCATCGTAGTGGCACCTC+TGG + chr2_4:20395380-20395399 None:intergenic 50.0%
!!! TGGAAGGTGTTTTTGGGGTG+TGG - chr2_4:20395751-20395770 Msa0320120:intron 50.0%
!!! AAGGTGTTTTTGGGGTGTGG+TGG - chr2_4:20395754-20395773 Msa0320120:intron 50.0%
!! GGTGTGGTGGAGAGGTTTTA+AGG - chr2_4:20395767-20395786 Msa0320120:intron 50.0%
!! GAGAGTGCTTCACATGCTTC+AGG + chr2_4:20396491-20396510 None:intergenic 50.0%
GCGGCAAAATGTTCAGCCAA+TGG + chr2_4:20396704-20396723 None:intergenic 50.0%
CAGTTTCATCTCGACACCGC+CGG - chr2_4:20393629-20393648 Msa0320120:three_prime_UTR 55.0%
! GACGGCGTCGAAAAGAAACG+CGG + chr2_4:20393708-20393727 None:intergenic 55.0%
! GCGTTTCTTTTCGACGCCGT+CGG - chr2_4:20393707-20393726 Msa0320120:three_prime_UTR 55.0%
!!! GTTTTTGGGGTGTGGTGGAG+AGG - chr2_4:20395759-20395778 Msa0320120:intron 55.0%
GGAGGATCGTGTTCCGAGTT+GGG + chr2_4:20395822-20395841 None:intergenic 55.0%
CAACTCGGAACACGATCCTC+CGG - chr2_4:20395821-20395840 Msa0320120:intron 55.0%
GCGGAGGTTACGCGAACTGA+CGG + chr2_4:20393591-20393610 None:intergenic 60.0%
GTCGGAGAGTGAACTCACGG+CGG + chr2_4:20393610-20393629 None:intergenic 60.0%
CGGAGGATCGTGTTCCGAGT+TGG + chr2_4:20395823-20395842 None:intergenic 60.0%
GGAGAGTGAACTCACGGCGG+AGG + chr2_4:20393607-20393626 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 20393522 20396897 20393522 ID=Msa0320120;Name=Msa0320120
chr2_4 mRNA 20393522 20396897 20393522 ID=Msa0320120-mRNA-1;Parent=Msa0320120;Name=Msa0320120-mRNA-1;_AED=0.07;_eAED=0.23;_QI=0|0.4|0.5|1|0|0.16|6|653|161
chr2_4 exon 20393522 20393976 20393522 ID=Msa0320120-mRNA-1:exon:7544;Parent=Msa0320120-mRNA-1
chr2_4 exon 20394502 20394725 20394502 ID=Msa0320120-mRNA-1:exon:7543;Parent=Msa0320120-mRNA-1
chr2_4 exon 20394894 20394959 20394894 ID=Msa0320120-mRNA-1:exon:7542;Parent=Msa0320120-mRNA-1
chr2_4 exon 20394992 20395097 20394992 ID=Msa0320120-mRNA-1:exon:7541;Parent=Msa0320120-mRNA-1
chr2_4 exon 20396097 20396181 20396097 ID=Msa0320120-mRNA-1:exon:7540;Parent=Msa0320120-mRNA-1
chr2_4 exon 20396695 20396897 20396695 ID=Msa0320120-mRNA-1:exon:7539;Parent=Msa0320120-mRNA-1
chr2_4 CDS 20396695 20396897 20396695 ID=Msa0320120-mRNA-1:cds;Parent=Msa0320120-mRNA-1
chr2_4 CDS 20396097 20396181 20396097 ID=Msa0320120-mRNA-1:cds;Parent=Msa0320120-mRNA-1
chr2_4 CDS 20394992 20395097 20394992 ID=Msa0320120-mRNA-1:cds;Parent=Msa0320120-mRNA-1
chr2_4 CDS 20394894 20394959 20394894 ID=Msa0320120-mRNA-1:cds;Parent=Msa0320120-mRNA-1
chr2_4 CDS 20394700 20394725 20394700 ID=Msa0320120-mRNA-1:cds;Parent=Msa0320120-mRNA-1
chr2_4 three_prime_UTR 20394502 20394699 20394502 ID=Msa0320120-mRNA-1:three_prime_utr;Parent=Msa0320120-mRNA-1
chr2_4 three_prime_UTR 20393522 20393976 20393522 ID=Msa0320120-mRNA-1:three_prime_utr;Parent=Msa0320120-mRNA-1
Gene Sequence

>Msa0320120

ATGCCAGAAATTTACCTCAGATCTGTTTCACCTAAAAAATTGTGTCGCATCTCTCTCTCATCGACGCCGTCAGTTCGCGTAACCTCCGCCGTGAGTTCACTCTCCGACAGTTTCATCTCGACACCGCCGGTACAGTTTAGTTTACTCTCCGCCATTCTTACTCGACGTTGCTGGTACAAATCACCGCGTTTCTTTTCGACGCCGTGGTCACTTGGAGCTATTGTGCATGAAATGTTGGTGGGATATCCACCTTTTTATTCTGATGATCCAATGTCAACATGTAGGAAGATGGGTGATTCAACCAACATCCCTTCTTCGTCTAGTGAACTTAAAAAAAGAAAGGCCAGAGGTGCCACTACGATGAAAAAAATTACCACCAAAGTTCTGAAATCAGAAACTGGGAGGTGTTATGGTCAAAATTCTGCCAAGTTTAAGAGTTATGCAGCATTTCTTGCTCGAAAAGCATTTTGTTATTCTAGAAAGTAA
Protein sequence

>Msa0320120

MPEIYLRSVSPKKLCRISLSSTPSVRVTSAVSSLSDSFISTPPVQFSLLSAILTRRCWYKSPRFFSTPWSLGAIVHEMLVGYPPFYSDDPMSTCRKMGDSTNIPSSSSELKKRKARGATTMKKITTKVLKSETGRCYGQNSAKFKSYAAFLARKAFCYSRK*