AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1201370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1201370 MtrunA17_Chr8g0371351 98.295 528 7 2 1 527 1 527 0.0 1066
Msa1201370 MtrunA17_Chr5g0406001 49.785 466 208 11 21 474 33 484 1.04e-154 452
Msa1201370 MtrunA17_Chr8g0371371 50.325 461 212 10 21 475 33 482 1.73e-152 446
Msa1201370 MtrunA17_Chr5g0425141 38.306 496 274 13 24 495 22 509 2.63e-95 299
Msa1201370 MtrunA17_Chr3g0124421 40.263 457 250 16 27 470 27 473 9.99e-94 295
Msa1201370 MtrunA17_Chr2g0320621 29.139 453 278 18 24 450 2 437 1.74e-33 133
Msa1201370 MtrunA17_Chr7g0221681 29.231 390 225 17 92 450 64 433 4.46e-31 125
Msa1201370 MtrunA17_Chr4g0027681 28.723 376 226 14 101 450 77 436 2.20e-30 124
Msa1201370 MtrunA17_Chr1g0179751 27.007 411 263 12 65 450 71 469 2.44e-30 124
Msa1201370 MtrunA17_Chr7g0249451 29.897 485 250 27 1 450 1 430 3.72e-30 123
Msa1201370 MtrunA17_Chr1g0189381 26.877 413 253 18 65 450 39 429 2.42e-28 117
Msa1201370 MtrunA17_Chr7g0265331 26.694 487 288 21 65 522 45 491 4.74e-28 117
Msa1201370 MtrunA17_Chr6g0484731 25.330 379 235 13 101 450 191 550 3.17e-26 112
Msa1201370 MtrunA17_Chr2g0310831 28.142 366 218 14 106 450 99 440 1.04e-25 109
Msa1201370 MtrunA17_Chr2g0310821 26.463 393 242 15 73 450 82 442 3.43e-23 102
Msa1201370 MtrunA17_Chr3g0130931 26.243 362 228 12 106 450 109 448 6.53e-22 98.6
Msa1201370 MtrunA17_Chr4g0010951 25.610 410 226 17 101 453 61 448 1.31e-21 97.8
Msa1201370 MtrunA17_Chr2g0308001 25.936 374 230 14 106 455 63 413 1.37e-21 97.4
Msa1201370 MtrunA17_Chr5g0419291 24.775 444 277 19 27 453 28 431 1.76e-21 97.1
Msa1201370 MtrunA17_Chr2g0310801 25.275 364 228 15 106 450 95 433 1.01e-20 94.7
Msa1201370 MtrunA17_Chr4g0053721 24.737 380 232 16 102 453 80 433 1.27e-20 95.5
Msa1201370 MtrunA17_Chr3g0130741 26.509 381 230 16 95 450 79 434 1.98e-20 94.0
Msa1201370 MtrunA17_Chr2g0307991 28.150 373 208 20 106 450 64 404 1.30e-19 91.3
Msa1201370 MtrunA17_Chr3g0095891 24.947 469 265 21 30 453 35 461 6.61e-19 89.4
Msa1201370 MtrunA17_Chr5g0401601 23.545 378 239 15 102 453 88 441 1.27e-17 86.3
Msa1201370 MtrunA17_Chr5g0435871 23.769 467 292 20 13 453 9 437 1.41e-17 85.1
Msa1201370 MtrunA17_Chr1g0210201 25.676 370 220 12 102 450 132 467 1.57e-17 85.1
Msa1201370 MtrunA17_Chr8g0352661 25.867 375 227 16 105 453 90 439 2.42e-17 84.3
Msa1201370 MtrunA17_Chr8g0352671 23.769 467 293 19 13 453 10 439 3.06e-17 84.3
Msa1201370 MtrunA17_Chr8g0386441 25.067 371 228 15 105 453 90 432 1.57e-16 82.0
Msa1201370 MtrunA17_Chr4g0001991 27.248 367 227 16 102 450 173 517 5.48e-16 80.9
Msa1201370 MtrunA17_Chr5g0442921 25.000 396 222 18 102 453 192 556 6.72e-16 80.5
Msa1201370 MtrunA17_Chr5g0395871 25.132 378 235 15 94 450 74 424 8.75e-16 79.7
Msa1201370 MtrunA17_Chr5g0395931 27.397 365 219 18 108 450 95 435 2.16e-15 78.6
Msa1201370 MtrunA17_Chr6g0484901 24.451 364 231 10 102 450 157 491 3.79e-14 74.7
Msa1201370 MtrunA17_Chr2g0307461 26.963 382 208 19 108 447 77 429 6.80e-14 73.9
Msa1201370 MtrunA17_Chr3g0087851 24.603 378 231 17 99 450 87 436 7.51e-14 73.6
Msa1201370 MtrunA17_Chr3g0099901 26.087 368 228 15 102 450 143 485 7.33e-13 70.9
Msa1201370 MtrunA17_Chr6g0486981 24.523 367 228 13 108 450 95 436 9.80e-13 70.1
Msa1201370 MtrunA17_Chr7g0239001 25.445 393 216 19 105 450 63 425 1.05e-12 70.1
Msa1201370 MtrunA17_Chr1g0179091 28.151 238 149 10 226 450 138 366 1.19e-12 69.7
Msa1201370 MtrunA17_Chr2g0311451 23.404 376 220 15 108 450 95 435 1.52e-12 69.7
Msa1201370 MtrunA17_Chr6g0457401 23.077 377 238 14 98 450 88 436 4.62e-12 68.2
Msa1201370 MtrunA17_Chr8g0390761 23.362 351 221 16 106 437 121 442 7.51e-12 67.4
Msa1201370 MtrunA17_Chr7g0275731 25.067 371 228 16 102 450 142 484 8.92e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1201370 AT4G35880.1 64.829 526 173 5 4 524 5 523 0.0 680
Msa1201370 AT4G35880.2 70.163 429 118 4 4 427 5 428 0.0 616
Msa1201370 AT2G17760.1 54.362 447 193 4 18 462 24 461 2.09e-169 489
Msa1201370 AT3G51330.1 45.495 444 229 6 18 454 20 457 1.25e-130 390
Msa1201370 AT3G51330.3 45.682 440 226 6 18 450 20 453 2.28e-130 387
Msa1201370 AT3G51360.1 44.615 455 223 10 27 474 22 454 1.82e-124 374
Msa1201370 AT3G51360.9 44.615 455 223 10 27 474 32 464 3.70e-124 373
Msa1201370 AT3G51350.1 44.695 443 227 10 21 454 23 456 2.34e-123 372
Msa1201370 AT3G51340.2 42.952 454 241 8 7 454 10 451 3.02e-123 371
Msa1201370 AT3G51340.1 40.725 469 242 9 7 454 10 463 9.84e-115 350
Msa1201370 AT3G51330.2 43.301 418 224 6 18 428 20 431 9.32e-112 340
Msa1201370 AT3G51360.7 45.503 378 191 6 101 474 4 370 1.71e-109 332
Msa1201370 AT3G51360.10 43.659 410 207 9 27 429 22 414 7.97e-108 329
Msa1201370 AT3G51360.6 43.659 410 207 9 27 429 32 424 1.26e-107 328
Msa1201370 AT3G51360.5 43.077 390 202 7 27 413 32 404 1.10e-102 315
Msa1201370 AT3G51360.4 43.077 390 202 7 27 413 22 394 1.20e-102 315
Msa1201370 AT3G51340.3 40.887 406 223 7 7 407 10 403 4.73e-100 308
Msa1201370 AT5G10080.1 39.375 480 255 16 24 475 22 493 9.20e-98 306
Msa1201370 AT3G51340.4 42.857 371 199 6 7 372 10 372 2.04e-95 295
Msa1201370 AT3G51360.3 46.012 326 164 5 153 474 11 328 4.25e-93 288
Msa1201370 AT3G51360.8 46.012 326 164 5 153 474 11 328 4.25e-93 288
Msa1201370 AT3G51360.2 44.582 323 159 7 27 346 32 337 1.49e-85 268
Msa1201370 AT3G02740.2 30.053 376 217 16 101 450 9 364 1.72e-33 132
Msa1201370 AT3G02740.1 30.053 376 217 16 101 450 84 439 2.68e-33 132
Msa1201370 AT5G36260.1 27.098 417 259 16 57 450 39 433 2.39e-30 124
Msa1201370 AT1G65240.1 28.822 399 240 18 75 450 49 426 3.40e-30 123
Msa1201370 AT1G49050.2 26.485 404 236 18 97 461 25 406 1.87e-29 120
Msa1201370 AT2G36670.4 29.427 384 218 16 98 450 30 391 9.30e-29 119
Msa1201370 AT2G36670.3 29.427 384 218 16 98 450 30 391 9.30e-29 119
Msa1201370 AT2G36670.1 28.738 428 243 18 63 450 57 462 1.32e-28 119
Msa1201370 AT5G22850.1 27.470 415 256 18 63 450 44 440 3.95e-28 117
Msa1201370 AT2G36670.2 29.134 381 217 16 101 450 99 457 8.92e-28 117
Msa1201370 AT1G49050.1 26.500 400 233 18 101 461 202 579 2.07e-27 116
Msa1201370 AT1G08210.1 26.972 393 246 17 101 469 83 458 3.97e-27 115
Msa1201370 AT1G08210.4 26.972 393 246 17 101 469 117 492 4.61e-27 115
Msa1201370 AT1G08210.3 26.972 393 246 17 101 469 131 506 5.35e-27 115
Msa1201370 AT1G05840.1 27.252 433 253 22 50 450 36 438 2.18e-26 112
Msa1201370 AT1G08210.2 25.476 420 245 18 101 469 83 485 1.10e-23 104
Msa1201370 AT3G50050.1 25.128 390 241 16 102 471 92 450 1.33e-22 102
Msa1201370 AT5G43100.2 24.641 418 255 18 62 453 37 420 4.40e-22 99.8
Msa1201370 AT5G43100.1 24.641 418 255 18 62 453 37 420 5.59e-22 100
Msa1201370 AT4G33490.2 25.450 389 232 14 95 453 54 414 8.00e-22 98.6
Msa1201370 AT1G77480.1 26.087 391 230 16 98 457 64 426 1.42e-21 98.2
Msa1201370 AT1G77480.2 26.302 384 224 16 98 450 64 419 1.59e-21 97.4
Msa1201370 AT3G54400.1 26.087 368 212 16 107 450 93 424 2.53e-21 97.1
Msa1201370 AT1G44130.1 26.190 378 215 17 106 450 53 399 8.75e-21 95.1
Msa1201370 AT2G23945.1 23.711 388 238 15 101 457 95 455 2.17e-20 94.4
Msa1201370 AT1G49050.3 27.698 278 160 12 101 357 202 459 1.04e-19 92.4
Msa1201370 AT5G10770.1 27.540 374 227 15 96 450 125 473 1.95e-19 91.7
Msa1201370 AT3G25700.1 25.628 398 215 19 102 450 84 449 3.06e-19 90.9
Msa1201370 AT2G03200.1 26.110 383 210 17 106 450 111 458 5.12e-19 90.1
Msa1201370 AT4G30030.1 24.259 371 230 13 106 454 82 423 3.75e-18 87.4
Msa1201370 AT3G12700.1 23.200 375 242 12 102 450 106 460 4.54e-18 87.4
Msa1201370 AT4G33490.1 24.202 376 232 13 95 445 51 398 1.84e-17 85.1
Msa1201370 AT2G28040.1 25.556 360 215 16 106 450 69 390 2.20e-17 84.7
Msa1201370 AT2G28040.2 25.556 360 215 16 106 450 60 381 2.23e-17 84.7
Msa1201370 AT5G33340.1 26.087 368 222 15 106 450 94 434 2.66e-17 84.7
Msa1201370 AT5G45120.1 25.882 425 210 17 102 450 83 478 1.51e-16 82.8
Msa1201370 AT5G10760.1 27.596 366 226 17 97 450 126 464 2.90e-16 81.6
Msa1201370 AT3G52500.1 23.797 395 232 14 105 450 93 467 3.07e-16 81.6
Msa1201370 AT3G18490.1 26.431 367 224 14 102 450 162 500 3.36e-15 78.6
Msa1201370 AT2G42980.1 24.163 418 246 19 74 453 143 527 3.69e-15 78.6
Msa1201370 AT1G64830.1 26.415 424 261 18 51 450 32 428 4.77e-15 77.8
Msa1201370 AT1G01300.1 24.468 376 224 15 102 450 142 484 8.14e-15 77.4
Msa1201370 AT1G25510.1 25.410 366 226 13 102 450 148 483 6.84e-14 74.3
Msa1201370 AT2G28010.1 23.140 363 221 13 106 450 69 391 1.92e-13 72.8
Msa1201370 AT2G28220.1 22.826 368 230 14 101 450 66 397 4.55e-13 71.6
Msa1201370 AT5G07030.1 23.577 369 221 15 108 450 121 454 1.39e-12 70.1
Msa1201370 AT3G61820.1 25.397 378 224 17 102 450 135 483 3.02e-12 69.3
Msa1201370 AT1G09750.1 27.224 371 212 18 107 450 109 448 7.93e-12 67.8
Msa1201370 AT2G39710.1 25.065 387 215 17 105 445 68 425 2.38e-11 66.2
Msa1201370 AT5G02190.1 24.093 386 222 17 111 450 82 442 8.92e-11 64.7

Find 160 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGAGGCAAATGTCATATT+TGG 0.121470 8_1:+54574095 Msa1201370:CDS
TGTGATGGGTTCACATTAAA+TGG 0.190870 8_1:-54574430 None:intergenic
TGAATGTTGTGAATTGTATT+TGG 0.224651 8_1:-54575589 None:intergenic
GATCATTCATTTATAGTTTA+CGG 0.262012 8_1:+54575706 Msa1201370:exon
GATTCATTCTCCATGTGTTT+TGG 0.265087 8_1:+54574346 Msa1201370:CDS
ATACAGGAAGTGACTTGTTT+TGG 0.276629 8_1:+54573197 Msa1201370:CDS
GATTTAAGGTGAAACCTTAC+AGG 0.283183 8_1:-54575923 None:intergenic
CCCTAGCATGAGTTTAACTA+TGG 0.294423 8_1:+54574948 Msa1201370:CDS
AGAAAGAGAGGATCTTGTTT+TGG 0.297684 8_1:-54572516 None:intergenic
GACACCTGGAGTAAAGTTTA+TGG 0.303372 8_1:+54573165 Msa1201370:CDS
AGTGCTGAGCTGAACATAAT+TGG 0.309833 8_1:+54575162 Msa1201370:CDS
CATTATACAACAATTGAGTT+AGG 0.311886 8_1:+54573142 Msa1201370:CDS
GTGCCTCCTGCAGTCGCTGC+GGG 0.316913 8_1:+54575528 Msa1201370:CDS
TGACCGGGAAAAGCTCATAT+TGG 0.330953 8_1:+54575341 Msa1201370:CDS
TTGAATATTGTTATGATATG+AGG 0.331633 8_1:+54574822 Msa1201370:CDS
CTATAGAGCAAGAAAATAAA+AGG 0.334111 8_1:-54575772 None:intergenic
AAGCGAGACCGGCGTCGAAT+TGG 0.339323 8_1:-54572840 None:intergenic
TTGATTGATTGTGATGTTAT+AGG 0.358144 8_1:-54574558 None:intergenic
TTACTTGTTGAAGATCCATT+AGG 0.359898 8_1:-54573906 None:intergenic
GACCGGGAAAAGCTCATATT+GGG 0.361609 8_1:+54575342 Msa1201370:CDS
TTCCCATCGATGGCCGGAAA+AGG 0.365257 8_1:+54572753 Msa1201370:CDS
TGGATCTTCAACAAGTAAAA+AGG 0.367601 8_1:+54573911 Msa1201370:CDS
AACATCCTCCACCAGTATTC+CGG 0.372461 8_1:-54574036 None:intergenic
TTGAGGCAAATGTCATATTT+GGG 0.373533 8_1:+54574096 Msa1201370:CDS
TGCGTGGAGATAATAATTAT+TGG 0.374493 8_1:-54575000 None:intergenic
GCATTGGAAGAATAAGCTTT+GGG 0.389645 8_1:+54574377 Msa1201370:CDS
ACTTCACTTCTGACTTGCTT+TGG 0.395375 8_1:+54575642 Msa1201370:CDS
CTTAGTGTATATAATCCTAA+TGG 0.396178 8_1:+54573891 Msa1201370:CDS
GGACCATTTCTTGACGGGTT+CGG 0.396839 8_1:-54572713 None:intergenic
AACCTCTACATCCGGAATAC+TGG 0.406049 8_1:+54574025 Msa1201370:CDS
GGCATTGGAAGAATAAGCTT+TGG 0.408549 8_1:+54574376 Msa1201370:CDS
CGGTTTGTTTGGACTTGGTA+TGG 0.411015 8_1:+54574276 Msa1201370:CDS
TTTATGGTGGCACTTGATAC+AGG 0.412986 8_1:+54573181 Msa1201370:CDS
AGCGAGACCGGCGTCGAATT+GGG 0.413335 8_1:-54572839 None:intergenic
ATTTGCTTCTGCGTTGGCTT+CGG 0.413723 8_1:+54573264 Msa1201370:CDS
CCTAACGGTTTGTTTGGACT+TGG 0.414197 8_1:+54574271 Msa1201370:CDS
ACCTTCCGTATCAGCTCCCT+TGG 0.415434 8_1:+54572880 Msa1201370:CDS
TTCATTCCCAAGTGGAAGAT+AGG 0.418720 8_1:+54574771 Msa1201370:CDS
ACTCTCGGAAAGCCTTGAAT+AGG 0.420275 8_1:-54574666 None:intergenic
GCTGCTCCTAACGGTTTGTT+TGG 0.422219 8_1:+54574265 Msa1201370:CDS
TATTCCGGATGTAGAGGTTT+CGG 0.423673 8_1:-54574021 None:intergenic
ACTCCGAACCCGTCAAGAAA+TGG 0.424803 8_1:+54572710 Msa1201370:CDS
CATAATAATGCCATCCCAAC+AGG 0.431829 8_1:+54575489 Msa1201370:CDS
ATATCATAACAATATTCAAA+AGG 0.433640 8_1:-54574818 None:intergenic
GGTAAAGTTAGCTCATATAA+TGG 0.435481 8_1:+54575727 Msa1201370:three_prime_UTR
ATCAAGCTAGTATTTGAATC+AGG 0.440142 8_1:-54574925 None:intergenic
TATCTGAGTGTTGTCCTGTT+GGG 0.442424 8_1:-54575503 None:intergenic
TAATTCAATTTAGCCCTGTA+AGG 0.443316 8_1:+54575909 Msa1201370:three_prime_UTR
TTTCTGCTTGAATCTGTGGC+AGG 0.445297 8_1:-54575564 None:intergenic
CAGTTCCTAGCATGTTATCA+AGG 0.451307 8_1:+54574308 Msa1201370:CDS
CCAAGCCCCGCAGCGACTGC+AGG 0.451663 8_1:-54575534 None:intergenic
ATTCAGCAACTGCCCTTACA+TGG 0.453310 8_1:+54573983 Msa1201370:CDS
TGGTTGATCCAACCTATTCA+AGG 0.456361 8_1:+54574654 Msa1201370:CDS
TCAAAAGGGATCCTTGAATC+AGG 0.458347 8_1:-54574803 None:intergenic
ACGCACCGCAACCAATGCCT+TGG 0.464658 8_1:+54573957 Msa1201370:CDS
GTCATGGTTATCATCTGGTT+GGG 0.469543 8_1:-54574069 None:intergenic
CGCACCGCAACCAATGCCTT+GGG 0.470136 8_1:+54573958 Msa1201370:CDS
GAGTGGGACCATTTCTTGAC+GGG 0.471515 8_1:-54572718 None:intergenic
TGAACATCTTGTGCAATCAC+AGG 0.473018 8_1:-54573224 None:intergenic
GTCTACGGCCGCGGAGGAAG+CGG 0.475524 8_1:-54572813 None:intergenic
TTCTGCATTTGCTTCTGCGT+TGG 0.479332 8_1:+54573258 Msa1201370:CDS
ATTATACAACAATTGAGTTA+GGG 0.483927 8_1:+54573143 Msa1201370:CDS
TCCCATCGATGGCCGGAAAA+GGG 0.485538 8_1:+54572754 Msa1201370:CDS
AGCCCCGCAGCGACTGCAGG+AGG 0.489204 8_1:-54575531 None:intergenic
GGACTTTCTGCTTGAATCTG+TGG 0.491123 8_1:-54575568 None:intergenic
ACAAGGTCATGGTTATCATC+TGG 0.491895 8_1:-54574074 None:intergenic
TCTTGACGGGTTCGGAGTAA+CGG 0.492465 8_1:-54572705 None:intergenic
GGGAAGGTGAAGGTGTGGAG+TGG 0.492527 8_1:-54572735 None:intergenic
GAGACATAAGAAACCATGTA+AGG 0.493294 8_1:-54573996 None:intergenic
CGTCGAATTGGGAGAGTCTA+CGG 0.494067 8_1:-54572828 None:intergenic
TTGGATCATAAACAACAAAA+CGG 0.494783 8_1:-54574981 None:intergenic
GGAGTGGGACCATTTCTTGA+CGG 0.498257 8_1:-54572719 None:intergenic
TTATCTGAGTGTTGTCCTGT+TGG 0.500735 8_1:-54575504 None:intergenic
GGCGGCGTCCACCTGATTCA+AGG 0.508785 8_1:+54574792 Msa1201370:CDS
TATCATAACAATATTCAAAA+GGG 0.509072 8_1:-54574817 None:intergenic
GGTCATGGTTATCATCTGGT+TGG 0.509682 8_1:-54574070 None:intergenic
GCGGTCACGGTCAGCTAACT+CGG 0.509711 8_1:-54572794 None:intergenic
GGAATTACCACTTACTGTGA+TGG 0.510180 8_1:-54574445 None:intergenic
ATTGCAGAAAACTTCATGAC+TGG 0.511221 8_1:+54575303 Msa1201370:intron
GGCCGCGGAGGAAGCGGTCA+CGG 0.513673 8_1:-54572807 None:intergenic
ACAATCCAAGGGAGCTGATA+CGG 0.515182 8_1:-54572885 None:intergenic
GCCGTCGGAGAAAGCGAGAC+CGG 0.516971 8_1:-54572851 None:intergenic
TGCTGAATGTCCCAAGGCAT+TGG 0.521268 8_1:-54573968 None:intergenic
TTCACCTTCCCATCGATGGC+CGG 0.522545 8_1:+54572747 Msa1201370:CDS
ATCAACCAAATAAGTAAAAG+AGG 0.523671 8_1:-54574639 None:intergenic
TTAGATGTTGCTGCTCCTAA+CGG 0.523739 8_1:+54574256 Msa1201370:CDS
TCCGCCGAAACCTCTACATC+CGG 0.525784 8_1:+54574017 Msa1201370:CDS
CACCTTCACCTTCCCATCGA+TGG 0.530680 8_1:+54572743 Msa1201370:CDS
CCAAGTCCAAACAAACCGTT+AGG 0.530750 8_1:-54574271 None:intergenic
TTGATTGTGATGTTATAGGT+AGG 0.539595 8_1:-54574554 None:intergenic
GGAAGGTGAAGGTGTGGAGT+GGG 0.540282 8_1:-54572734 None:intergenic
GGTTGGTTACATACAATCCA+AGG 0.547001 8_1:-54572897 None:intergenic
GCCGGTCTCGCTTTCTCCGA+CGG 0.547301 8_1:+54572850 Msa1201370:CDS
CACCAGTATTCCGGATGTAG+AGG 0.550268 8_1:-54574027 None:intergenic
CCCATAGTTAAACTCATGCT+AGG 0.550350 8_1:-54574949 None:intergenic
TCGGAAAGCCTTGAATAGGT+TGG 0.551873 8_1:-54574662 None:intergenic
CCTTCCCTTGATAACATGCT+AGG 0.552068 8_1:-54574313 None:intergenic
AGAATAAGCTTTGGGGACAA+AGG 0.562087 8_1:+54574385 Msa1201370:CDS
ATGTCCCAAGGCATTGGTTG+CGG 0.563380 8_1:-54573962 None:intergenic
CCTAGCATGTTATCAAGGGA+AGG 0.563836 8_1:+54574313 Msa1201370:CDS
CTTGACAGGTTATGACATTG+AGG 0.564914 8_1:+54575464 Msa1201370:intron
GGTGCCTCCTGCAGTCGCTG+CGG 0.569484 8_1:+54575527 Msa1201370:CDS
TGAGTGTTGTCCTGTTGGGA+TGG 0.569912 8_1:-54575499 None:intergenic
ATTGGGAGAGTCTACGGCCG+CGG 0.572075 8_1:-54572822 None:intergenic
GCCACCATAAACTTTACTCC+AGG 0.575979 8_1:-54573169 None:intergenic
GAGTGTAATGTGAAAGGGAA+GGG 0.578140 8_1:-54572494 None:intergenic
AGTTCCTAGCATGTTATCAA+GGG 0.578402 8_1:+54574309 Msa1201370:CDS
CATTAGGATTATATACACTA+AGG 0.579056 8_1:-54573890 None:intergenic
TACATCCGGAATACTGGTGG+AGG 0.581327 8_1:+54574031 Msa1201370:CDS
AACAGGACAACACTCAGATA+AGG 0.581972 8_1:+54575506 Msa1201370:CDS
TGATTGTGATGTTATAGGTA+GGG 0.582602 8_1:-54574553 None:intergenic
GAATTACCACTTACTGTGAT+GGG 0.582853 8_1:-54574444 None:intergenic
CCATAGTTAAACTCATGCTA+GGG 0.587521 8_1:-54574948 None:intergenic
TTCTGCTTGAATCTGTGGCA+GGG 0.590184 8_1:-54575563 None:intergenic
ACAATTGAGTTAGGGACACC+TGG 0.591063 8_1:+54573151 Msa1201370:CDS
CTCTACATCCGGAATACTGG+TGG 0.591456 8_1:+54574028 Msa1201370:CDS
ATTGTATAATAGAGTCAATG+AGG 0.597973 8_1:-54575865 None:intergenic
CATTTGCCTCAACAAGGTCA+TGG 0.599237 8_1:-54574085 None:intergenic
CACAATCAATCAAGTCCGTG+TGG 0.602457 8_1:+54574568 Msa1201370:CDS
CCTAGCATGAGTTTAACTAT+GGG 0.603068 8_1:+54574949 Msa1201370:CDS
GCCATCCCGTCCAAAACACA+TGG 0.611680 8_1:-54574356 None:intergenic
ATGAGTTTAACTATGGGAGG+TGG 0.611842 8_1:+54574955 Msa1201370:CDS
AATAATTATTATCTCCACGC+AGG 0.613796 8_1:+54575002 Msa1201370:CDS
TGCCTCCTGCAGTCGCTGCG+GGG 0.614730 8_1:+54575529 Msa1201370:CDS
TAACTCGGCGTAGTATTCAA+AGG 0.615030 8_1:-54572779 None:intergenic
AAGGGAGCTGATACGGAAGG+TGG 0.615307 8_1:-54572878 None:intergenic
ATTTAAGGTGAAACCTTACA+GGG 0.619103 8_1:-54575922 None:intergenic
GGCAGTTGCTGAATGTCCCA+AGG 0.619484 8_1:-54573974 None:intergenic
GGCCATCGATGGGAAGGTGA+AGG 0.620111 8_1:-54572745 None:intergenic
AAAGGGATCCTTGAATCAGG+TGG 0.621177 8_1:-54574800 None:intergenic
CGATTAGAGTTGTTCCCACA+CGG 0.622058 8_1:-54574583 None:intergenic
GGGAAAAGCTCATATTGGGA+TGG 0.629078 8_1:+54575346 Msa1201370:CDS
GGAGTGTAATGTGAAAGGGA+AGG 0.632135 8_1:-54572495 None:intergenic
GAACATCTTGTGCAATCACA+GGG 0.634149 8_1:-54573223 None:intergenic
GGGAGAGTCTACGGCCGCGG+AGG 0.634309 8_1:-54572819 None:intergenic
AGCATGAGTTTAACTATGGG+AGG 0.640728 8_1:+54574952 Msa1201370:CDS
TCGATGGGAAGGTGAAGGTG+TGG 0.641944 8_1:-54572740 None:intergenic
ACGGAAGGTGGAATTGCCGT+CGG 0.643883 8_1:-54572866 None:intergenic
GTGCTCTAATATCAACATGT+CGG 0.645210 8_1:-54575750 None:intergenic
ACGCCGCCTATCTTCCACTT+GGG 0.645869 8_1:-54574777 None:intergenic
AGACTCTCCCAATTCGACGC+CGG 0.646984 8_1:+54572832 Msa1201370:CDS
ATATGACATTTGCCTCAACA+AGG 0.649605 8_1:-54574091 None:intergenic
TTGTATAATAGAGTCAATGA+GGG 0.650338 8_1:-54575864 None:intergenic
TCCGGATGTAGAGGTTTCGG+CGG 0.652058 8_1:-54574018 None:intergenic
TCCAAGGGAGCTGATACGGA+AGG 0.654290 8_1:-54572881 None:intergenic
AGCTGGCACTATTACCTGCG+TGG 0.655731 8_1:-54575016 None:intergenic
AGACATAAGAAACCATGTAA+GGG 0.656398 8_1:-54573995 None:intergenic
ATTCCCAAGTGGAAGATAGG+CGG 0.657278 8_1:+54574774 Msa1201370:CDS
TGATAACCATGACCTTGTTG+AGG 0.660761 8_1:+54574079 Msa1201370:CDS
TGACGGGTTCGGAGTAACGG+TGG 0.661765 8_1:-54572702 None:intergenic
GTTGCAGTTTCATTCCCAAG+TGG 0.664300 8_1:+54574763 Msa1201370:intron
ACAATCAATCAAGTCCGTGT+GGG 0.664422 8_1:+54574569 Msa1201370:CDS
CATTGGAAGAATAAGCTTTG+GGG 0.665699 8_1:+54574378 Msa1201370:CDS
GTAACGGTGGTGCATTGTGA+AGG 0.669458 8_1:-54572689 None:intergenic
GACCGTGACCGCTTCCTCCG+CGG 0.675079 8_1:+54572805 Msa1201370:CDS
GTTGGTTACATACAATCCAA+GGG 0.675864 8_1:-54572896 None:intergenic
AGCAAGTCAGAAGTGAAGTG+CGG 0.678400 8_1:-54575638 None:intergenic
ACCTGGAGTAAAGTTTATGG+TGG 0.683352 8_1:+54573168 Msa1201370:CDS
GGGAACAACTCTAATCGACG+TGG 0.725199 8_1:+54574589 Msa1201370:CDS
AGCTGTGGTCAAGTACAGAG+TGG 0.744828 8_1:+54574226 Msa1201370:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTAGTTTAAAAATCTC+AGG + chr8_1:54573700-54573719 Msa1201370:intron 15.0%
!! AATATATAGAATAATATGGA+TGG - chr8_1:54574153-54574172 None:intergenic 15.0%
!! TATCATAACAATATTCAAAA+GGG - chr8_1:54574820-54574839 None:intergenic 15.0%
!! ATATCATAACAATATTCAAA+AGG - chr8_1:54574821-54574840 None:intergenic 15.0%
!! AACAATAATAAATTAGAGAA+TGG - chr8_1:54575413-54575432 None:intergenic 15.0%
!!! TTCTTTTTCTTTTCATTCTT+CGG + chr8_1:54572535-54572554 Msa1201370:five_prime_UTR 20.0%
!!! AAATGTCTTGATATTTTTCT+TGG + chr8_1:54573043-54573062 Msa1201370:intron 20.0%
!!! TTTTCTTGGAATTTGATTTT+TGG + chr8_1:54573057-54573076 Msa1201370:intron 20.0%
!!! ATTTGTATCTTTTTTTGTGT+TGG + chr8_1:54573094-54573113 Msa1201370:intron 20.0%
!!! TTTGTATCTTTTTTTGTGTT+GGG + chr8_1:54573095-54573114 Msa1201370:intron 20.0%
!! ATTATACAACAATTGAGTTA+GGG + chr8_1:54573143-54573162 Msa1201370:CDS 20.0%
!! ATGATACATTTAACATATCA+TGG - chr8_1:54573799-54573818 None:intergenic 20.0%
!! AATGGAATGAATTATTATTG+TGG + chr8_1:54573856-54573875 Msa1201370:intron 20.0%
!!! ATTTTATTGCTAGTTATGAT+CGG - chr8_1:54574188-54574207 None:intergenic 20.0%
!! ATTAAACACGACTTTAAATT+CGG - chr8_1:54574492-54574511 None:intergenic 20.0%
!! TTGAATATTGTTATGATATG+AGG + chr8_1:54574822-54574841 Msa1201370:CDS 20.0%
!! AAGCTTAATATTCTTTCTTA+TGG + chr8_1:54574893-54574912 Msa1201370:intron 20.0%
!! GATCATTCATTTATAGTTTA+CGG + chr8_1:54575706-54575725 Msa1201370:exon 20.0%
! ATGAAAAGAAAAAGAAAGAG+AGG - chr8_1:54572531-54572550 None:intergenic 25.0%
!! TCTTTTTCTTTTCATTCTTC+GGG + chr8_1:54572536-54572555 Msa1201370:five_prime_UTR 25.0%
!!! TTTTTGGAAAGTGATGATTT+TGG + chr8_1:54573019-54573038 Msa1201370:intron 25.0%
!!! TTTTGGAAAGTGATGATTTT+GGG + chr8_1:54573020-54573039 Msa1201370:intron 25.0%
! CATTATACAACAATTGAGTT+AGG + chr8_1:54573142-54573161 Msa1201370:CDS 25.0%
! GTGATTTCTATGATATGTTA+AGG + chr8_1:54573381-54573400 Msa1201370:intron 25.0%
! TTATTTCCATAAACTCTCAA+GGG - chr8_1:54573540-54573559 None:intergenic 25.0%
!! TATGCTTTAAATCATCTGAT+AGG + chr8_1:54573619-54573638 Msa1201370:intron 25.0%
! AGTTTAAAAATCTCAGGAAT+TGG + chr8_1:54573706-54573725 Msa1201370:intron 25.0%
! CATTAGGATTATATACACTA+AGG - chr8_1:54573893-54573912 None:intergenic 25.0%
! CTTAGTGTATATAATCCTAA+TGG + chr8_1:54573891-54573910 Msa1201370:CDS 25.0%
! TTGATTGATTGTGATGTTAT+AGG - chr8_1:54574561-54574580 None:intergenic 25.0%
! ATCAACCAAATAAGTAAAAG+AGG - chr8_1:54574642-54574661 None:intergenic 25.0%
! AATAACAACATCTAGCTAAA+TGG - chr8_1:54574712-54574731 None:intergenic 25.0%
! TTGGATCATAAACAACAAAA+CGG - chr8_1:54574984-54575003 None:intergenic 25.0%
! GTTAGATACATAGATTTAAG+AGG - chr8_1:54575047-54575066 None:intergenic 25.0%
! TGAGTTTGATATATACTTTG+AGG + chr8_1:54575440-54575459 Msa1201370:intron 25.0%
! TGAATGTTGTGAATTGTATT+TGG - chr8_1:54575592-54575611 None:intergenic 25.0%
! CTATAGAGCAAGAAAATAAA+AGG - chr8_1:54575775-54575794 None:intergenic 25.0%
! TTGTATAATAGAGTCAATGA+GGG - chr8_1:54575867-54575886 None:intergenic 25.0%
! ATTGTATAATAGAGTCAATG+AGG - chr8_1:54575868-54575887 None:intergenic 25.0%
!!! TTTTGGAGTGTAATGTGAAA+GGG - chr8_1:54572502-54572521 None:intergenic 30.0%
!! ATGATGATTTTGGTGAAACT+GGG - chr8_1:54572613-54572632 None:intergenic 30.0%
!! GATGATGATGATGATGATTT+TGG - chr8_1:54572623-54572642 None:intergenic 30.0%
AATAGTGAGAATGAGGAAAA+GGG - chr8_1:54572922-54572941 None:intergenic 30.0%
TTTCCAAAAATAGAGTAGGT+TGG - chr8_1:54573009-54573028 None:intergenic 30.0%
TCACTTTCCAAAAATAGAGT+AGG - chr8_1:54573013-54573032 None:intergenic 30.0%
CTTATTTCCATAAACTCTCA+AGG - chr8_1:54573541-54573560 None:intergenic 30.0%
TTCAATTACAAAACACTCTC+AGG + chr8_1:54573664-54573683 Msa1201370:intron 30.0%
ATCTCAGGAATTGGTATATA+TGG + chr8_1:54573715-54573734 Msa1201370:intron 30.0%
! ATCTTGTGTTTTACTACTGT+TGG + chr8_1:54573822-54573841 Msa1201370:intron 30.0%
!!! CTGTTGGTTTTGTTTTTGAA+TGG + chr8_1:54573838-54573857 Msa1201370:intron 30.0%
GAATGAATTATTATTGTGGC+AGG + chr8_1:54573860-54573879 Msa1201370:intron 30.0%
! TTACTTGTTGAAGATCCATT+AGG - chr8_1:54573909-54573928 None:intergenic 30.0%
TGGATCTTCAACAAGTAAAA+AGG + chr8_1:54573911-54573930 Msa1201370:CDS 30.0%
AGACATAAGAAACCATGTAA+GGG - chr8_1:54573998-54574017 None:intergenic 30.0%
TTGAGGCAAATGTCATATTT+GGG + chr8_1:54574096-54574115 Msa1201370:CDS 30.0%
CTTTAAATTCGGATAATCAG+AGG - chr8_1:54574481-54574500 None:intergenic 30.0%
TCGAATATATCTCTTATGCT+TGG + chr8_1:54574517-54574536 Msa1201370:intron 30.0%
TGATTGTGATGTTATAGGTA+GGG - chr8_1:54574556-54574575 None:intergenic 30.0%
TTGATTGTGATGTTATAGGT+AGG - chr8_1:54574557-54574576 None:intergenic 30.0%
! TGGAACCTCTTTTACTTATT+TGG + chr8_1:54574634-54574653 Msa1201370:CDS 30.0%
CTGCAACATAAATACTTCAA+TGG - chr8_1:54574750-54574769 None:intergenic 30.0%
ATCAAGCTAGTATTTGAATC+AGG - chr8_1:54574928-54574947 None:intergenic 30.0%
TGCGTGGAGATAATAATTAT+TGG - chr8_1:54575003-54575022 None:intergenic 30.0%
AATAATTATTATCTCCACGC+AGG + chr8_1:54575002-54575021 Msa1201370:CDS 30.0%
ATCTAACAACTGTTAAGCTT+TGG + chr8_1:54575060-54575079 Msa1201370:intron 30.0%
TCTAACAACTGTTAAGCTTT+GGG + chr8_1:54575061-54575080 Msa1201370:intron 30.0%
ATGATACATCATGCATCATT+TGG - chr8_1:54575248-54575267 None:intergenic 30.0%
GGTAAAGTTAGCTCATATAA+TGG + chr8_1:54575727-54575746 Msa1201370:three_prime_UTR 30.0%
TAATTCAATTTAGCCCTGTA+AGG + chr8_1:54575909-54575928 Msa1201370:three_prime_UTR 30.0%
!!! GTTTTGGAGTGTAATGTGAA+AGG - chr8_1:54572503-54572522 None:intergenic 35.0%
!! AGAAAGAGAGGATCTTGTTT+TGG - chr8_1:54572519-54572538 None:intergenic 35.0%
!! TTCTTTTCATTCTTCGGGTT+TGG + chr8_1:54572541-54572560 Msa1201370:five_prime_UTR 35.0%
!! GATGATGATTTTGGTGAAAC+TGG - chr8_1:54572614-54572633 None:intergenic 35.0%
GTTGGTTACATACAATCCAA+GGG - chr8_1:54572899-54572918 None:intergenic 35.0%
ATAGTGAGAATGAGGAAAAG+GGG - chr8_1:54572921-54572940 None:intergenic 35.0%
GAATAGTGAGAATGAGGAAA+AGG - chr8_1:54572923-54572942 None:intergenic 35.0%
ATGGATGAATAGTGAGAATG+AGG - chr8_1:54572929-54572948 None:intergenic 35.0%
! TCACCAACCTACTCTATTTT+TGG + chr8_1:54573003-54573022 Msa1201370:intron 35.0%
!!! ATACAGGAAGTGACTTGTTT+TGG + chr8_1:54573197-54573216 Msa1201370:CDS 35.0%
!!! TACAGGAAGTGACTTGTTTT+GGG + chr8_1:54573198-54573217 Msa1201370:CDS 35.0%
AGCCATTTCTATGATATGTG+AGG + chr8_1:54573588-54573607 Msa1201370:intron 35.0%
GCCATTTCTATGATATGTGA+GGG + chr8_1:54573589-54573608 Msa1201370:intron 35.0%
TCCCTCACATATCATAGAAA+TGG - chr8_1:54573593-54573612 None:intergenic 35.0%
TAAATCATCTGATAGGATGC+TGG + chr8_1:54573626-54573645 Msa1201370:intron 35.0%
AAATCATCTGATAGGATGCT+GGG + chr8_1:54573627-54573646 Msa1201370:intron 35.0%
TCAGGAATTGGTATATATGG+TGG + chr8_1:54573718-54573737 Msa1201370:intron 35.0%
CAGGAATTGGTATATATGGT+GGG + chr8_1:54573719-54573738 Msa1201370:intron 35.0%
! GTATATATGGTGGGATTCTT+TGG + chr8_1:54573728-54573747 Msa1201370:intron 35.0%
GAGACATAAGAAACCATGTA+AGG - chr8_1:54573999-54574018 None:intergenic 35.0%
ATATGACATTTGCCTCAACA+AGG - chr8_1:54574094-54574113 None:intergenic 35.0%
GTTGAGGCAAATGTCATATT+TGG + chr8_1:54574095-54574114 Msa1201370:CDS 35.0%
! GCTAGTTATGATCGGTTTAA+AGG - chr8_1:54574180-54574199 None:intergenic 35.0%
AGTTCCTAGCATGTTATCAA+GGG + chr8_1:54574309-54574328 Msa1201370:CDS 35.0%
! GATTCATTCTCCATGTGTTT+TGG + chr8_1:54574346-54574365 Msa1201370:CDS 35.0%
GCATTGGAAGAATAAGCTTT+GGG + chr8_1:54574377-54574396 Msa1201370:CDS 35.0%
CATTGGAAGAATAAGCTTTG+GGG + chr8_1:54574378-54574397 Msa1201370:CDS 35.0%
!! TGTGATGGGTTCACATTAAA+TGG - chr8_1:54574433-54574452 None:intergenic 35.0%
GAATTACCACTTACTGTGAT+GGG - chr8_1:54574447-54574466 None:intergenic 35.0%
!! TTCACTGCTCTTTTTGATTC+TGG + chr8_1:54574614-54574633 Msa1201370:CDS 35.0%
!! TATTTTGCCACTTACACTCT+CGG - chr8_1:54574684-54574703 None:intergenic 35.0%
CCATAGTTAAACTCATGCTA+GGG - chr8_1:54574951-54574970 None:intergenic 35.0%
CCTAGCATGAGTTTAACTAT+GGG + chr8_1:54574949-54574968 Msa1201370:CDS 35.0%
AGATTTAAGAGGTTTCAAGC+TGG - chr8_1:54575036-54575055 None:intergenic 35.0%
CTAACAACTGTTAAGCTTTG+GGG + chr8_1:54575062-54575081 Msa1201370:intron 35.0%
TTCACTCTGTAGAAGTAGTA+AGG - chr8_1:54575115-54575134 None:intergenic 35.0%
CATTTGGATTTCTGCATTCA+CGG - chr8_1:54575232-54575251 None:intergenic 35.0%
ATTGCAGAAAACTTCATGAC+TGG + chr8_1:54575303-54575322 Msa1201370:intron 35.0%
GGAAGAAATCTGATTGTAAG+TGG + chr8_1:54575367-54575386 Msa1201370:intron 35.0%
GTGCTCTAATATCAACATGT+CGG - chr8_1:54575753-54575772 None:intergenic 35.0%
GAGTGTAATGTGAAAGGGAA+GGG - chr8_1:54572497-54572516 None:intergenic 40.0%
! TATTCAAAGGAGCCCTTTTC+CGG - chr8_1:54572769-54572788 None:intergenic 40.0%
TAACTCGGCGTAGTATTCAA+AGG - chr8_1:54572782-54572801 None:intergenic 40.0%
! GGTTGGTTACATACAATCCA+AGG - chr8_1:54572900-54572919 None:intergenic 40.0%
GACACCTGGAGTAAAGTTTA+TGG + chr8_1:54573165-54573184 Msa1201370:CDS 40.0%
ACCTGGAGTAAAGTTTATGG+TGG + chr8_1:54573168-54573187 Msa1201370:CDS 40.0%
TTTATGGTGGCACTTGATAC+AGG + chr8_1:54573181-54573200 Msa1201370:CDS 40.0%
GAACATCTTGTGCAATCACA+GGG - chr8_1:54573226-54573245 None:intergenic 40.0%
TGAACATCTTGTGCAATCAC+AGG - chr8_1:54573227-54573246 None:intergenic 40.0%
ATGATATGTTAAGGCCCTGT+TGG + chr8_1:54573390-54573409 Msa1201370:intron 40.0%
TGATATGTTAAGGCCCTGTT+GGG + chr8_1:54573391-54573410 Msa1201370:intron 40.0%
TAAGCTGATAATCCCCAACA+GGG - chr8_1:54573407-54573426 None:intergenic 40.0%
TTAAGCTGATAATCCCCAAC+AGG - chr8_1:54573408-54573427 None:intergenic 40.0%
CTATGATATGTGAGGGATGA+CGG + chr8_1:54573596-54573615 Msa1201370:intron 40.0%
AATCATCTGATAGGATGCTG+GGG + chr8_1:54573628-54573647 Msa1201370:intron 40.0%
TATTCCGGATGTAGAGGTTT+CGG - chr8_1:54574024-54574043 None:intergenic 40.0%
GTCATGGTTATCATCTGGTT+GGG - chr8_1:54574072-54574091 None:intergenic 40.0%
ACAAGGTCATGGTTATCATC+TGG - chr8_1:54574077-54574096 None:intergenic 40.0%
TGATAACCATGACCTTGTTG+AGG + chr8_1:54574079-54574098 Msa1201370:CDS 40.0%
! ATACATGTTTTCTGCAGCTG+TGG + chr8_1:54574211-54574230 Msa1201370:intron 40.0%
TTAGATGTTGCTGCTCCTAA+CGG + chr8_1:54574256-54574275 Msa1201370:CDS 40.0%
CAGTTCCTAGCATGTTATCA+AGG + chr8_1:54574308-54574327 Msa1201370:CDS 40.0%
!! CATTCTCCATGTGTTTTGGA+CGG + chr8_1:54574350-54574369 Msa1201370:CDS 40.0%
!! ATTCTCCATGTGTTTTGGAC+GGG + chr8_1:54574351-54574370 Msa1201370:CDS 40.0%
! GGCATTGGAAGAATAAGCTT+TGG + chr8_1:54574376-54574395 Msa1201370:CDS 40.0%
!! AGAATAAGCTTTGGGGACAA+AGG + chr8_1:54574385-54574404 Msa1201370:CDS 40.0%
GGAATTACCACTTACTGTGA+TGG - chr8_1:54574448-54574467 None:intergenic 40.0%
ATAATCAGAGGCAAGACACA+GGG - chr8_1:54574469-54574488 None:intergenic 40.0%
ACAATCAATCAAGTCCGTGT+GGG + chr8_1:54574569-54574588 Msa1201370:CDS 40.0%
TGGTTGATCCAACCTATTCA+AGG + chr8_1:54574654-54574673 Msa1201370:CDS 40.0%
TTCATTCCCAAGTGGAAGAT+AGG + chr8_1:54574771-54574790 Msa1201370:CDS 40.0%
TCAAAAGGGATCCTTGAATC+AGG - chr8_1:54574806-54574825 None:intergenic 40.0%
CCCTAGCATGAGTTTAACTA+TGG + chr8_1:54574948-54574967 Msa1201370:CDS 40.0%
CCCATAGTTAAACTCATGCT+AGG - chr8_1:54574952-54574971 None:intergenic 40.0%
AGCATGAGTTTAACTATGGG+AGG + chr8_1:54574952-54574971 Msa1201370:CDS 40.0%
ATGAGTTTAACTATGGGAGG+TGG + chr8_1:54574955-54574974 Msa1201370:CDS 40.0%
! AGTGCTGAGCTGAACATAAT+TGG + chr8_1:54575162-54575181 Msa1201370:CDS 40.0%
ATATGATCGTTCTCATGCCT+CGG + chr8_1:54575268-54575287 Msa1201370:intron 40.0%
!!! GTTACCGTGTTGTTTTTGAC+CGG + chr8_1:54575325-54575344 Msa1201370:CDS 40.0%
!!! TTACCGTGTTGTTTTTGACC+GGG + chr8_1:54575326-54575345 Msa1201370:CDS 40.0%
TTTCCCGGTCAAAAACAACA+CGG - chr8_1:54575332-54575351 None:intergenic 40.0%
! ATCCCAATATGAGCTTTTCC+CGG - chr8_1:54575347-54575366 None:intergenic 40.0%
!! ATATACTTTGAGGCCTTGAC+AGG + chr8_1:54575450-54575469 Msa1201370:intron 40.0%
CTCAATGTCATAACCTGTCA+AGG - chr8_1:54575466-54575485 None:intergenic 40.0%
CTTGACAGGTTATGACATTG+AGG + chr8_1:54575464-54575483 Msa1201370:intron 40.0%
CATAATAATGCCATCCCAAC+AGG + chr8_1:54575489-54575508 Msa1201370:CDS 40.0%
! TATCTGAGTGTTGTCCTGTT+GGG - chr8_1:54575506-54575525 None:intergenic 40.0%
! TTATCTGAGTGTTGTCCTGT+TGG - chr8_1:54575507-54575526 None:intergenic 40.0%
AACAGGACAACACTCAGATA+AGG + chr8_1:54575506-54575525 Msa1201370:CDS 40.0%
!! ACTTCACTTCTGACTTGCTT+TGG + chr8_1:54575642-54575661 Msa1201370:CDS 40.0%
GGAGTGTAATGTGAAAGGGA+AGG - chr8_1:54572498-54572517 None:intergenic 45.0%
ACAATCCAAGGGAGCTGATA+CGG - chr8_1:54572888-54572907 None:intergenic 45.0%
TGAGAATGAGGAAAAGGGGT+TGG - chr8_1:54572917-54572936 None:intergenic 45.0%
ACAATTGAGTTAGGGACACC+TGG + chr8_1:54573151-54573170 Msa1201370:CDS 45.0%
GCCACCATAAACTTTACTCC+AGG - chr8_1:54573172-54573191 None:intergenic 45.0%
TTCTGCATTTGCTTCTGCGT+TGG + chr8_1:54573258-54573277 Msa1201370:CDS 45.0%
!! ATTTGCTTCTGCGTTGGCTT+CGG + chr8_1:54573264-54573283 Msa1201370:CDS 45.0%
GATATGTTAAGGCCCTGTTG+GGG + chr8_1:54573392-54573411 Msa1201370:intron 45.0%
AAGCTGCCCTTGAGAGTTTA+TGG + chr8_1:54573531-54573550 Msa1201370:intron 45.0%
ATTCAGCAACTGCCCTTACA+TGG + chr8_1:54573983-54574002 Msa1201370:CDS 45.0%
AACCTCTACATCCGGAATAC+TGG + chr8_1:54574025-54574044 Msa1201370:CDS 45.0%
AACATCCTCCACCAGTATTC+CGG - chr8_1:54574039-54574058 None:intergenic 45.0%
GGTCATGGTTATCATCTGGT+TGG - chr8_1:54574073-54574092 None:intergenic 45.0%
CATTTGCCTCAACAAGGTCA+TGG - chr8_1:54574088-54574107 None:intergenic 45.0%
CCAAGTCCAAACAAACCGTT+AGG - chr8_1:54574274-54574293 None:intergenic 45.0%
! CCTAACGGTTTGTTTGGACT+TGG + chr8_1:54574271-54574290 Msa1201370:CDS 45.0%
! CGGTTTGTTTGGACTTGGTA+TGG + chr8_1:54574276-54574295 Msa1201370:CDS 45.0%
! CCTTCCCTTGATAACATGCT+AGG - chr8_1:54574316-54574335 None:intergenic 45.0%
CCTAGCATGTTATCAAGGGA+AGG + chr8_1:54574313-54574332 Msa1201370:CDS 45.0%
TGTGAACCCATCACAGTAAG+TGG + chr8_1:54574438-54574457 Msa1201370:intron 45.0%
GATAATCAGAGGCAAGACAC+AGG - chr8_1:54574470-54574489 None:intergenic 45.0%
CACAATCAATCAAGTCCGTG+TGG + chr8_1:54574568-54574587 Msa1201370:CDS 45.0%
CGATTAGAGTTGTTCCCACA+CGG - chr8_1:54574586-54574605 None:intergenic 45.0%
TCGGAAAGCCTTGAATAGGT+TGG - chr8_1:54574665-54574684 None:intergenic 45.0%
ACTCTCGGAAAGCCTTGAAT+AGG - chr8_1:54574669-54574688 None:intergenic 45.0%
GTTGCAGTTTCATTCCCAAG+TGG + chr8_1:54574763-54574782 Msa1201370:intron 45.0%
ATTCCCAAGTGGAAGATAGG+CGG + chr8_1:54574774-54574793 Msa1201370:CDS 45.0%
AAAGGGATCCTTGAATCAGG+TGG - chr8_1:54574803-54574822 None:intergenic 45.0%
TGACCGGGAAAAGCTCATAT+TGG + chr8_1:54575341-54575360 Msa1201370:CDS 45.0%
GACCGGGAAAAGCTCATATT+GGG + chr8_1:54575342-54575361 Msa1201370:CDS 45.0%
GGGAAAAGCTCATATTGGGA+TGG + chr8_1:54575346-54575365 Msa1201370:CDS 45.0%
TTCTGCTTGAATCTGTGGCA+GGG - chr8_1:54575566-54575585 None:intergenic 45.0%
TTTCTGCTTGAATCTGTGGC+AGG - chr8_1:54575567-54575586 None:intergenic 45.0%
GGACTTTCTGCTTGAATCTG+TGG - chr8_1:54575571-54575590 None:intergenic 45.0%
AGCAAGTCAGAAGTGAAGTG+CGG - chr8_1:54575641-54575660 None:intergenic 45.0%
!!! GAATTTTTAATAATAAAAAA+TGG - chr8_1:54572948-54572967 None:intergenic 5.0%
!! ATAAAATATATAGAATAATA+TGG - chr8_1:54574157-54574176 None:intergenic 5.0%
GTAACGGTGGTGCATTGTGA+AGG - chr8_1:54572692-54572711 None:intergenic 50.0%
!! TCTTGACGGGTTCGGAGTAA+CGG - chr8_1:54572708-54572727 None:intergenic 50.0%
ACTCCGAACCCGTCAAGAAA+TGG + chr8_1:54572710-54572729 Msa1201370:CDS 50.0%
!! GGACCATTTCTTGACGGGTT+CGG - chr8_1:54572716-54572735 None:intergenic 50.0%
GAGTGGGACCATTTCTTGAC+GGG - chr8_1:54572721-54572740 None:intergenic 50.0%
GGAGTGGGACCATTTCTTGA+CGG - chr8_1:54572722-54572741 None:intergenic 50.0%
CGTCGAATTGGGAGAGTCTA+CGG - chr8_1:54572831-54572850 None:intergenic 50.0%
!! ATGTCCCAAGGCATTGGTTG+CGG - chr8_1:54573965-54573984 None:intergenic 50.0%
TGCTGAATGTCCCAAGGCAT+TGG - chr8_1:54573971-54573990 None:intergenic 50.0%
CACCAGTATTCCGGATGTAG+AGG - chr8_1:54574030-54574049 None:intergenic 50.0%
CTCTACATCCGGAATACTGG+TGG + chr8_1:54574028-54574047 Msa1201370:CDS 50.0%
TACATCCGGAATACTGGTGG+AGG + chr8_1:54574031-54574050 Msa1201370:CDS 50.0%
AGCTGTGGTCAAGTACAGAG+TGG + chr8_1:54574226-54574245 Msa1201370:intron 50.0%
! GCTGCTCCTAACGGTTTGTT+TGG + chr8_1:54574265-54574284 Msa1201370:CDS 50.0%
!! TCCATGTGTTTTGGACGGGA+TGG + chr8_1:54574355-54574374 Msa1201370:CDS 50.0%
!! TGTTTTGGACGGGATGGCAT+TGG + chr8_1:54574361-54574380 Msa1201370:CDS 50.0%
GGGAACAACTCTAATCGACG+TGG + chr8_1:54574589-54574608 Msa1201370:CDS 50.0%
! AGGCTTTCCGAGAGTGTAAG+TGG + chr8_1:54574674-54574693 Msa1201370:intron 50.0%
GCAATCAACATCAAGAGCCG+AGG - chr8_1:54575288-54575307 None:intergenic 50.0%
! TGAGTGTTGTCCTGTTGGGA+TGG - chr8_1:54575502-54575521 None:intergenic 50.0%
! GGAAGGTGAAGGTGTGGAGT+GGG - chr8_1:54572737-54572756 None:intergenic 55.0%
TCGATGGGAAGGTGAAGGTG+TGG - chr8_1:54572743-54572762 None:intergenic 55.0%
CACCTTCACCTTCCCATCGA+TGG + chr8_1:54572743-54572762 Msa1201370:CDS 55.0%
TTCACCTTCCCATCGATGGC+CGG + chr8_1:54572747-54572766 Msa1201370:CDS 55.0%
! TTTTCCGGCCATCGATGGGA+AGG - chr8_1:54572754-54572773 None:intergenic 55.0%
TTCCCATCGATGGCCGGAAA+AGG + chr8_1:54572753-54572772 Msa1201370:CDS 55.0%
TCCCATCGATGGCCGGAAAA+GGG + chr8_1:54572754-54572773 Msa1201370:CDS 55.0%
AGACTCTCCCAATTCGACGC+CGG + chr8_1:54572832-54572851 Msa1201370:CDS 55.0%
ACGGAAGGTGGAATTGCCGT+CGG - chr8_1:54572869-54572888 None:intergenic 55.0%
AAGGGAGCTGATACGGAAGG+TGG - chr8_1:54572881-54572900 None:intergenic 55.0%
ACCTTCCGTATCAGCTCCCT+TGG + chr8_1:54572880-54572899 Msa1201370:CDS 55.0%
TCCAAGGGAGCTGATACGGA+AGG - chr8_1:54572884-54572903 None:intergenic 55.0%
TCTGATAGGATGCTGGGGAC+AGG + chr8_1:54573633-54573652 Msa1201370:intron 55.0%
GGCAGTTGCTGAATGTCCCA+AGG - chr8_1:54573977-54573996 None:intergenic 55.0%
TCCGCCGAAACCTCTACATC+CGG + chr8_1:54574017-54574036 Msa1201370:CDS 55.0%
TCCGGATGTAGAGGTTTCGG+CGG - chr8_1:54574021-54574040 None:intergenic 55.0%
GCCATCCCGTCCAAAACACA+TGG - chr8_1:54574359-54574378 None:intergenic 55.0%
ACGCCGCCTATCTTCCACTT+GGG - chr8_1:54574780-54574799 None:intergenic 55.0%
AGCTGGCACTATTACCTGCG+TGG - chr8_1:54575019-54575038 None:intergenic 55.0%
!! TGACGGGTTCGGAGTAACGG+TGG - chr8_1:54572705-54572724 None:intergenic 60.0%
! GGGAAGGTGAAGGTGTGGAG+TGG - chr8_1:54572738-54572757 None:intergenic 60.0%
GGCCATCGATGGGAAGGTGA+AGG - chr8_1:54572748-54572767 None:intergenic 60.0%
! GCCCTTTTCCGGCCATCGAT+GGG - chr8_1:54572758-54572777 None:intergenic 60.0%
! AGCCCTTTTCCGGCCATCGA+TGG - chr8_1:54572759-54572778 None:intergenic 60.0%
GCGGTCACGGTCAGCTAACT+CGG - chr8_1:54572797-54572816 None:intergenic 60.0%
ATTGGGAGAGTCTACGGCCG+CGG - chr8_1:54572825-54572844 None:intergenic 60.0%
! AGCGAGACCGGCGTCGAATT+GGG - chr8_1:54572842-54572861 None:intergenic 60.0%
! AAGCGAGACCGGCGTCGAAT+TGG - chr8_1:54572843-54572862 None:intergenic 60.0%
ACGCACCGCAACCAATGCCT+TGG + chr8_1:54573957-54573976 Msa1201370:CDS 60.0%
CGCACCGCAACCAATGCCTT+GGG + chr8_1:54573958-54573977 Msa1201370:CDS 60.0%
GACGCCGCCTATCTTCCACT+TGG - chr8_1:54574781-54574800 None:intergenic 60.0%
GCCGGTCTCGCTTTCTCCGA+CGG + chr8_1:54572850-54572869 Msa1201370:CDS 65.0%
GCCGTCGGAGAAAGCGAGAC+CGG - chr8_1:54572854-54572873 None:intergenic 65.0%
GGCGGCGTCCACCTGATTCA+AGG + chr8_1:54574792-54574811 Msa1201370:CDS 65.0%
GACCGTGACCGCTTCCTCCG+CGG + chr8_1:54572805-54572824 Msa1201370:CDS 70.0%
GTCTACGGCCGCGGAGGAAG+CGG - chr8_1:54572816-54572835 None:intergenic 70.0%
GGTGCCTCCTGCAGTCGCTG+CGG + chr8_1:54575527-54575546 Msa1201370:CDS 70.0%
GTGCCTCCTGCAGTCGCTGC+GGG + chr8_1:54575528-54575547 Msa1201370:CDS 70.0%
TGCCTCCTGCAGTCGCTGCG+GGG + chr8_1:54575529-54575548 Msa1201370:CDS 70.0%
GGCCGCGGAGGAAGCGGTCA+CGG - chr8_1:54572810-54572829 None:intergenic 75.0%
GGGAGAGTCTACGGCCGCGG+AGG - chr8_1:54572822-54572841 None:intergenic 75.0%
AGCCCCGCAGCGACTGCAGG+AGG - chr8_1:54575534-54575553 None:intergenic 75.0%
CCAAGCCCCGCAGCGACTGC+AGG - chr8_1:54575537-54575556 None:intergenic 75.0%
CCTGCAGTCGCTGCGGGGCT+TGG + chr8_1:54575534-54575553 Msa1201370:CDS 75.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 54572483 54575935 54572483 ID=Msa1201370;Name=Msa1201370
chr8_1 mRNA 54572483 54575935 54572483 ID=Msa1201370-mRNA-1;Parent=Msa1201370;Name=Msa1201370-mRNA-1;_AED=0.12;_eAED=0.12;_QI=124|1|1|1|1|1|10|228|527
chr8_1 exon 54572483 54572905 54572483 ID=Msa1201370-mRNA-1:exon:17486;Parent=Msa1201370-mRNA-1
chr8_1 exon 54573138 54573285 54573138 ID=Msa1201370-mRNA-1:exon:17487;Parent=Msa1201370-mRNA-1
chr8_1 exon 54573882 54574117 54573882 ID=Msa1201370-mRNA-1:exon:17488;Parent=Msa1201370-mRNA-1
chr8_1 exon 54574228 54574452 54574228 ID=Msa1201370-mRNA-1:exon:17489;Parent=Msa1201370-mRNA-1
chr8_1 exon 54574553 54574688 54574553 ID=Msa1201370-mRNA-1:exon:17490;Parent=Msa1201370-mRNA-1
chr8_1 exon 54574770 54574843 54574770 ID=Msa1201370-mRNA-1:exon:17491;Parent=Msa1201370-mRNA-1
chr8_1 exon 54574924 54575023 54574924 ID=Msa1201370-mRNA-1:exon:17492;Parent=Msa1201370-mRNA-1
chr8_1 exon 54575129 54575186 54575129 ID=Msa1201370-mRNA-1:exon:17493;Parent=Msa1201370-mRNA-1
chr8_1 exon 54575310 54575381 54575310 ID=Msa1201370-mRNA-1:exon:17494;Parent=Msa1201370-mRNA-1
chr8_1 exon 54575472 54575935 54575472 ID=Msa1201370-mRNA-1:exon:17495;Parent=Msa1201370-mRNA-1
chr8_1 five_prime_UTR 54572483 54572606 54572483 ID=Msa1201370-mRNA-1:five_prime_utr;Parent=Msa1201370-mRNA-1
chr8_1 CDS 54572607 54572905 54572607 ID=Msa1201370-mRNA-1:cds;Parent=Msa1201370-mRNA-1
chr8_1 CDS 54573138 54573285 54573138 ID=Msa1201370-mRNA-1:cds;Parent=Msa1201370-mRNA-1
chr8_1 CDS 54573882 54574117 54573882 ID=Msa1201370-mRNA-1:cds;Parent=Msa1201370-mRNA-1
chr8_1 CDS 54574228 54574452 54574228 ID=Msa1201370-mRNA-1:cds;Parent=Msa1201370-mRNA-1
chr8_1 CDS 54574553 54574688 54574553 ID=Msa1201370-mRNA-1:cds;Parent=Msa1201370-mRNA-1
chr8_1 CDS 54574770 54574843 54574770 ID=Msa1201370-mRNA-1:cds;Parent=Msa1201370-mRNA-1
chr8_1 CDS 54574924 54575023 54574924 ID=Msa1201370-mRNA-1:cds;Parent=Msa1201370-mRNA-1
chr8_1 CDS 54575129 54575186 54575129 ID=Msa1201370-mRNA-1:cds;Parent=Msa1201370-mRNA-1
chr8_1 CDS 54575310 54575381 54575310 ID=Msa1201370-mRNA-1:cds;Parent=Msa1201370-mRNA-1
chr8_1 CDS 54575472 54575707 54575472 ID=Msa1201370-mRNA-1:cds;Parent=Msa1201370-mRNA-1
chr8_1 three_prime_UTR 54575708 54575935 54575708 ID=Msa1201370-mRNA-1:three_prime_utr;Parent=Msa1201370-mRNA-1
Gene Sequence

>Msa1201370

ATGCCCAGTTTCACCAAAATCATCATCATCATCATCTTAATCATTCTTCATCTTTCTATGTGTTGCAATGCTCACATATTCACCTTCACAATGCACCACCGTTACTCCGAACCCGTCAAGAAATGGTCCCACTCCACACCTTCACCTTCCCATCGATGGCCGGAAAAGGGCTCCTTTGAATACTACGCCGAGTTAGCTGACCGTGACCGCTTCCTCCGCGGCCGTAGACTCTCCCAATTCGACGCCGGTCTCGCTTTCTCCGACGGCAATTCCACCTTCCGTATCAGCTCCCTTGGATTTTTGCATTATACAACAATTGAGTTAGGGACACCTGGAGTAAAGTTTATGGTGGCACTTGATACAGGAAGTGACTTGTTTTGGGTACCCTGTGATTGCACAAGATGTTCAGCTACTCGTAGTTCTGCATTTGCTTCTGCGTTGGCTTCGGACTTTGACCTTAGTGTATATAATCCTAATGGATCTTCAACAAGTAAAAAGGTCACTTGTAACAATAGTCTCTGCACGCACCGCAACCAATGCCTTGGGACATTCAGCAACTGCCCTTACATGGTTTCTTATGTCTCCGCCGAAACCTCTACATCCGGAATACTGGTGGAGGATGTTCTGCACTTAACCCAACCAGATGATAACCATGACCTTGTTGAGGCAAATGTCATATTTGGCTGTGGTCAAGTACAGAGTGGATCATTCTTAGATGTTGCTGCTCCTAACGGTTTGTTTGGACTTGGTATGGAGAAAATATCAGTTCCTAGCATGTTATCAAGGGAAGGATTTACTGCAGATTCATTCTCCATGTGTTTTGGACGGGATGGCATTGGAAGAATAAGCTTTGGGGACAAAGGAAGTCTTGATCAAGATGAGACTCCATTTAATGTGAACCCATCACACCCTACCTATAACATCACAATCAATCAAGTCCGTGTGGGAACAACTCTAATCGACGTGGAATTCACTGCTCTTTTTGATTCTGGAACCTCTTTTACTTATTTGGTTGATCCAACCTATTCAAGGCTTTCCGAGAGTTTTCATTCCCAAGTGGAAGATAGGCGGCGTCCACCTGATTCAAGGATCCCTTTTGAATATTGTTATGATATGAGTCCTGATTCAAATACTAGCTTGATCCCTAGCATGAGTTTAACTATGGGAGGTGGAAGCCGTTTTGTTGTTTATGATCCAATAATTATTATCTCCACGCAGAGTGAACTTGTATATTGTCTAGCAGTAGTGAAGAGTGCTGAGCTGAACATAATTGGACAAAACTTCATGACTGGTTACCGTGTTGTTTTTGACCGGGAAAAGCTCATATTGGGATGGAAGAAATCTGATTGTTATGACATTGAGGATCATAATAATGCCATCCCAACAGGACAACACTCAGATAAGGTGCCTCCTGCAGTCGCTGCGGGGCTTGGCGATTACCCTGCCACAGATTCAAGCAGAAAGTCCAAATACAATTCACAACATTCAAGTGCATCACAGTCGTCGCTTTATAGCCGCACTTCACTTCTGACTTGCTTTGGATTTCTAATTTCTTATATTTTGTTTTGTTTACATGTTCTATGA
Protein sequence

>Msa1201370

MPSFTKIIIIIILIILHLSMCCNAHIFTFTMHHRYSEPVKKWSHSTPSPSHRWPEKGSFEYYAELADRDRFLRGRRLSQFDAGLAFSDGNSTFRISSLGFLHYTTIELGTPGVKFMVALDTGSDLFWVPCDCTRCSATRSSAFASALASDFDLSVYNPNGSSTSKKVTCNNSLCTHRNQCLGTFSNCPYMVSYVSAETSTSGILVEDVLHLTQPDDNHDLVEANVIFGCGQVQSGSFLDVAAPNGLFGLGMEKISVPSMLSREGFTADSFSMCFGRDGIGRISFGDKGSLDQDETPFNVNPSHPTYNITINQVRVGTTLIDVEFTALFDSGTSFTYLVDPTYSRLSESFHSQVEDRRRPPDSRIPFEYCYDMSPDSNTSLIPSMSLTMGGGSRFVVYDPIIIISTQSELVYCLAVVKSAELNIIGQNFMTGYRVVFDREKLILGWKKSDCYDIEDHNNAIPTGQHSDKVPPAVAAGLGDYPATDSSRKSKYNSQHSSASQSSLYSRTSLLTCFGFLISYILFCLHVL*