AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1202990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1202990 MtrunA17_Chr8g0373451 96.078 102 4 0 1 102 1 102 2.63e-65 192
Msa1202990 MtrunA17_Chr3g0143691 42.593 108 56 3 1 102 1 108 1.15e-15 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1202990 AT1G20460.1 42.453 106 57 3 1 102 1 106 1.14e-14 64.3
Msa1202990 AT1G76185.1 42.453 106 57 3 1 102 1 106 4.57e-12 57.8

Find 44 sgRNAs with CRISPR-Local

Find 325 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTTGTTAGTTAGTTAAAA+AGG 0.180464 8_1:-56737455 Msa1202990:five_prime_UTR
CGAGTGTTACAAGTAAAATA+AGG 0.239823 8_1:+56733454 None:intergenic
TATATCTTACATAATTAATT+TGG 0.249603 8_1:-56733604 Msa1202990:three_prime_UTR
ACTTGTAACACTCGTAAAAT+TGG 0.284676 8_1:-56733445 Msa1202990:three_prime_UTR
ATTCACTTAGCTTGTGCATA+TGG 0.310277 8_1:+56733486 None:intergenic
AGCAGAAGATCCTGGGAAAA+AGG 0.316556 8_1:+56733362 None:intergenic
TCATACGCCTAAGAGCTTCT+AGG 0.326975 8_1:-56733728 Msa1202990:CDS
CCAAGTAATGATATGAGGTT+TGG 0.331279 8_1:+56737330 None:intergenic
ATAAAGGGTCAAGCAGGATA+TGG 0.382041 8_1:+56733803 None:intergenic
CTGTTCAACAAGTTTAGAAC+GGG 0.430395 8_1:+56737411 None:intergenic
GGCAAACCTTGGCAAGGAAG+AGG 0.430808 8_1:-56733707 Msa1202990:CDS
AGGTTTGCCTAGAAGCTCTT+AGG 0.436059 8_1:+56733721 None:intergenic
GATCTGAGACAAACACGGTT+TGG 0.440546 8_1:-56737501 Msa1202990:five_prime_UTR
ACATAAACACAACCATTTCA+CGG 0.443810 8_1:+56737549 None:intergenic
CAAGTAATGATATGAGGTTT+GGG 0.444710 8_1:+56737331 None:intergenic
TTCACTTAGCTTGTGCATAT+GGG 0.450193 8_1:+56733487 None:intergenic
TCTTTCCCTCTTCCTTGCCA+AGG 0.451193 8_1:+56733701 None:intergenic
CACCACAAAGAATGCTCATA+TGG 0.453210 8_1:+56733665 None:intergenic
CCAAACCTCATATCATTACT+TGG 0.454335 8_1:-56737330 Msa1202990:intron
TGACAGCACTCAAATATTCA+TGG 0.456413 8_1:+56733577 None:intergenic
AATGTCACAGCAGAAGATCC+TGG 0.464356 8_1:+56733354 None:intergenic
ATTTATGCTGAGATCGGATC+TGG 0.468524 8_1:+56737377 None:intergenic
ATCACATCTCTATACTACTT+TGG 0.480081 8_1:+56733312 None:intergenic
TGCTGTGACATTTCTAGCAT+AGG 0.492979 8_1:-56733343 Msa1202990:three_prime_UTR
GAGTGTTACAAGTAAAATAA+GGG 0.495682 8_1:+56733455 None:intergenic
AATTACCAAGTAATGATATG+AGG 0.499125 8_1:+56737325 None:intergenic
GTCCATATGAGCATTCTTTG+TGG 0.501670 8_1:-56733667 Msa1202990:exon
GAGAATATTTATGCTGAGAT+CGG 0.512331 8_1:+56737371 None:intergenic
GAAATTTCATGCTTCTCTGT+AGG 0.523780 8_1:+56733421 None:intergenic
CCTAAAATAAAGGGTCAAGC+AGG 0.550751 8_1:+56733797 None:intergenic
AGTGCTGTCATTACTAGTGT+AGG 0.555939 8_1:-56733564 Msa1202990:three_prime_UTR
ATGTCACAGCAGAAGATCCT+GGG 0.557119 8_1:+56733355 None:intergenic
TTAATAAATGAATGAAGAAG+AGG 0.559434 8_1:+56737612 None:intergenic
GCAAACCTTGGCAAGGAAGA+GGG 0.560246 8_1:-56733706 Msa1202990:CDS
TTGGGAGAGAAGAAAGTGAG+AGG 0.560855 8_1:+56737349 None:intergenic
TTGCAGGGTTGATGTTGCTG+TGG 0.577557 8_1:-56733862 Msa1202990:intron
TCTTTGTGGTGATATCTCAC+AGG 0.583891 8_1:-56733653 Msa1202990:three_prime_UTR
AAGAGCTTCTAGGCAAACCT+TGG 0.584867 8_1:-56733718 Msa1202990:CDS
GCTGTTCAACAAGTTTAGAA+CGG 0.592461 8_1:+56737410 None:intergenic
CTTCTAGGCAAACCTTGGCA+AGG 0.624301 8_1:-56733713 Msa1202990:CDS
AAGAATGCTCATATGGACAA+TGG 0.637377 8_1:+56733672 None:intergenic
GGATGTACACTGGCAGACAA+AGG 0.645230 8_1:-56733632 Msa1202990:three_prime_UTR
ATATCTCACAGGATGTACAC+TGG 0.678712 8_1:-56733642 Msa1202990:three_prime_UTR
TATATGATCTGAGACAAACA+CGG 0.765222 8_1:-56737506 Msa1202990:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTATTTTTTAAAAAAACT+TGG - chr8_1:56733863-56733882 Msa1202990:intron 10.0%
!! TATATATAATATGTCAAAAT+GGG + chr8_1:56735011-56735030 None:intergenic 10.0%
!! ATATATATAATATGTCAAAA+TGG + chr8_1:56735012-56735031 None:intergenic 10.0%
!!! CATATTATATATATGTTTTT+AGG - chr8_1:56735018-56735037 Msa1202990:intron 10.0%
!!! CCAAATATTTTTTAAAAAAA+TGG + chr8_1:56735487-56735506 None:intergenic 10.0%
!!! CCATTTTTTTAAAAAATATT+TGG - chr8_1:56735484-56735503 Msa1202990:intron 10.0%
!! AATTCATACAAATATATAAA+AGG - chr8_1:56736183-56736202 Msa1202990:intron 10.0%
!! TATATCTTACATAATTAATT+TGG - chr8_1:56737289-56737308 Msa1202990:intron 10.0%
!!! CTATTTTTAAAAGAAAAACA+GGG + chr8_1:56734118-56734137 None:intergenic 15.0%
!!! AAAAGTTTTGTTTGTTTAAA+AGG - chr8_1:56734252-56734271 Msa1202990:intron 15.0%
!!! AAAGTTTTGTTTGTTTAAAA+GGG - chr8_1:56734253-56734272 Msa1202990:intron 15.0%
!! ATATATAATATGTCAAAATG+GGG + chr8_1:56735010-56735029 None:intergenic 15.0%
!! AAATTGAATTCCATATATTT+AGG + chr8_1:56735247-56735266 None:intergenic 15.0%
!!! TTTAACCATATGAAATTTTA+AGG - chr8_1:56735320-56735339 Msa1202990:intron 15.0%
!!! TCATTGTATTTAATTTAACA+GGG + chr8_1:56735656-56735675 None:intergenic 15.0%
!! ATATATTTGTATGAATTCAT+TGG + chr8_1:56736180-56736199 None:intergenic 15.0%
!!! TGTATTTTGATATGATAAAA+AGG - chr8_1:56736465-56736484 Msa1202990:intron 15.0%
!! AGTTTCTTCTATAATTTATT+TGG + chr8_1:56736809-56736828 None:intergenic 15.0%
!! TTAATAAATGAATGAAGAAG+AGG + chr8_1:56733284-56733303 None:intergenic 20.0%
!! AAGTTGTTAGTTAGTTAAAA+AGG - chr8_1:56733438-56733457 Msa1202990:three_prime_UTR 20.0%
!! AGTTGTTAGTTAGTTAAAAA+GGG - chr8_1:56733439-56733458 Msa1202990:three_prime_UTR 20.0%
!! GTAAAAAAAAAAAAAGTGAG+AGG + chr8_1:56733600-56733619 None:intergenic 20.0%
!!! TTTTAAAAAAACTTGGTATC+TGG - chr8_1:56733870-56733889 Msa1202990:intron 20.0%
!!! CCTATTTTTAAAAGAAAAAC+AGG + chr8_1:56734119-56734138 None:intergenic 20.0%
!!! CCTGTTTTTCTTTTAAAAAT+AGG - chr8_1:56734116-56734135 Msa1202990:intron 20.0%
!!! AATTCCATATATTTAGGATT+GGG + chr8_1:56735241-56735260 None:intergenic 20.0%
!!! TTTTGCCTTAAAATTTCATA+TGG + chr8_1:56735328-56735347 None:intergenic 20.0%
!!! TAGTATTGAAAATTTGAAAG+CGG + chr8_1:56735404-56735423 None:intergenic 20.0%
!!! GTCATTGTATTTAATTTAAC+AGG + chr8_1:56735657-56735676 None:intergenic 20.0%
!! TTCCTTACTATAGAATATAT+AGG + chr8_1:56736741-56736760 None:intergenic 20.0%
!!! TGATTTTTGATCAAATACTA+TGG - chr8_1:56736862-56736881 Msa1202990:intron 20.0%
!!! TATTGTAGATTTTTACAATG+AGG - chr8_1:56737503-56737522 Msa1202990:five_prime_UTR 20.0%
!!! AAAACTGGGTTTTGATTTTT+AGG - chr8_1:56733249-56733268 Msa1202990:three_prime_UTR 25.0%
!!! AAACTGGGTTTTGATTTTTA+GGG - chr8_1:56733250-56733269 Msa1202990:three_prime_UTR 25.0%
! AATTACCAAGTAATGATATG+AGG + chr8_1:56733571-56733590 None:intergenic 25.0%
! TTTGCTCTCTAGATTTAAAT+GGG + chr8_1:56733922-56733941 None:intergenic 25.0%
! TACAAAATGTCCTCAAATTA+CGG - chr8_1:56734539-56734558 Msa1202990:intron 25.0%
!!! AAGATTGATAGTTTTTCGAT+TGG - chr8_1:56734684-56734703 Msa1202990:intron 25.0%
!!! AGATTGATAGTTTTTCGATT+GGG - chr8_1:56734685-56734704 Msa1202990:intron 25.0%
! ATGTAAATTCTTGACATTGA+GGG - chr8_1:56734805-56734824 Msa1202990:intron 25.0%
!!! ATTCAGGTTGCATATAAATA+TGG - chr8_1:56735068-56735087 Msa1202990:intron 25.0%
! TACCTAATAGGTAATAAATG+AGG - chr8_1:56735170-56735189 Msa1202990:intron 25.0%
!! ATTCCATATATTTAGGATTG+GGG + chr8_1:56735240-56735259 None:intergenic 25.0%
!! GAATTCCATATATTTAGGAT+TGG + chr8_1:56735242-56735261 None:intergenic 25.0%
! CTTAAATCAAAAGGGTAAAT+GGG + chr8_1:56735291-56735310 None:intergenic 25.0%
! AAAATGAAATATTAGCCCAA+AGG - chr8_1:56735353-56735372 Msa1202990:intron 25.0%
!!! GGTTTGTCTTTGAAATAAAA+TGG - chr8_1:56735455-56735474 Msa1202990:intron 25.0%
! GTTTGATTGTTCAAGAAAAA+TGG + chr8_1:56735598-56735617 None:intergenic 25.0%
!! TTTAATTTAACAGGGAAGAA+GGG + chr8_1:56735648-56735667 None:intergenic 25.0%
!! ATTTAATTTAACAGGGAAGA+AGG + chr8_1:56735649-56735668 None:intergenic 25.0%
! ACTATAAGTTAGCATATTTG+AGG + chr8_1:56735687-56735706 None:intergenic 25.0%
! TAAAGTACAAAACCATTGAT+TGG - chr8_1:56735796-56735815 Msa1202990:intron 25.0%
! GGATGTAAAAATTTATGACT+CGG - chr8_1:56735976-56735995 Msa1202990:intron 25.0%
! GATGTAAAAATTTATGACTC+GGG - chr8_1:56735977-56735996 Msa1202990:intron 25.0%
! TCACATGGTTTAAATTTCTT+AGG - chr8_1:56736349-56736368 Msa1202990:intron 25.0%
!! TATTTTATAGGACAGATTGT+TGG - chr8_1:56736538-56736557 Msa1202990:intron 25.0%
!! ATTTTATAGGACAGATTGTT+GGG - chr8_1:56736539-56736558 Msa1202990:intron 25.0%
!! TTATCTGTCTTTTCTACAAT+AGG + chr8_1:56736681-56736700 None:intergenic 25.0%
! CAGATAAATCTTGATTTAGA+TGG - chr8_1:56736694-56736713 Msa1202990:intron 25.0%
! GACCTATATATTCTATAGTA+AGG - chr8_1:56736736-56736755 Msa1202990:intron 25.0%
! CGTATAAAATACATTGAAGA+TGG - chr8_1:56736961-56736980 Msa1202990:intron 25.0%
! AAAATACATTGAAGATGGTT+AGG - chr8_1:56736966-56736985 Msa1202990:intron 25.0%
! AGAAATTAAGGAAATTGTCA+AGG + chr8_1:56737012-56737031 None:intergenic 25.0%
! AATTTCCTTAATTTCTTGCA+GGG - chr8_1:56737016-56737035 Msa1202990:intron 25.0%
! GAGTGTTACAAGTAAAATAA+GGG + chr8_1:56737441-56737460 None:intergenic 25.0%
!!! AACTGGGTTTTGATTTTTAG+GGG - chr8_1:56733251-56733270 Msa1202990:three_prime_UTR 30.0%
!! ACTGTATTTTTACGATTTGG+CGG + chr8_1:56733308-56733327 None:intergenic 30.0%
!! GAGACTGTATTTTTACGATT+TGG + chr8_1:56733311-56733330 None:intergenic 30.0%
ACATAAACACAACCATTTCA+CGG + chr8_1:56733347-56733366 None:intergenic 30.0%
! TATATGATCTGAGACAAACA+CGG - chr8_1:56733387-56733406 Msa1202990:three_prime_UTR 30.0%
GAGAATATTTATGCTGAGAT+CGG + chr8_1:56733525-56733544 None:intergenic 30.0%
CAAGTAATGATATGAGGTTT+GGG + chr8_1:56733565-56733584 None:intergenic 30.0%
! TCTAAAACAACTACTTGTGT+CGG + chr8_1:56733752-56733771 None:intergenic 30.0%
CTTTGCTCTCTAGATTTAAA+TGG + chr8_1:56733923-56733942 None:intergenic 30.0%
ACTTCGTATATTGAAAGTGA+TGG - chr8_1:56734069-56734088 Msa1202990:intron 30.0%
TAGTCACAAATTATGCCTAT+GGG + chr8_1:56734283-56734302 None:intergenic 30.0%
ATAGTCACAAATTATGCCTA+TGG + chr8_1:56734284-56734303 None:intergenic 30.0%
TGATTGAACTCTTCATATTC+AGG - chr8_1:56734341-56734360 Msa1202990:intron 30.0%
!! ATAGTTTTTCGATTGGGTTA+TGG - chr8_1:56734691-56734710 Msa1202990:intron 30.0%
!! TAGTTTTTCGATTGGGTTAT+GGG - chr8_1:56734692-56734711 Msa1202990:intron 30.0%
CTCAATGTCAAGAATTTACA+TGG + chr8_1:56734806-56734825 None:intergenic 30.0%
CATGTAAATTCTTGACATTG+AGG - chr8_1:56734804-56734823 Msa1202990:intron 30.0%
! TAGCATTGAGATAAAATGAC+AGG - chr8_1:56734831-56734850 Msa1202990:intron 30.0%
TATTCATTCACTTTCAAACC+CGG - chr8_1:56734863-56734882 Msa1202990:intron 30.0%
TTAAGGTTATGGAGTTATTC+AGG - chr8_1:56735052-56735071 Msa1202990:intron 30.0%
! ATAGGTTTTCGTTACCTAAT+AGG - chr8_1:56735158-56735177 Msa1202990:intron 30.0%
AGCCTCATTTATTACCTATT+AGG + chr8_1:56735175-56735194 None:intergenic 30.0%
TAATAGGTAATAAATGAGGC+TGG - chr8_1:56735174-56735193 Msa1202990:intron 30.0%
! TTCCATATATTTAGGATTGG+GGG + chr8_1:56735239-56735258 None:intergenic 30.0%
GCTTAAATCAAAAGGGTAAA+TGG + chr8_1:56735292-56735311 None:intergenic 30.0%
TGCAAAGGCTTAAATCAAAA+GGG + chr8_1:56735299-56735318 None:intergenic 30.0%
TTCATATGGTTAAACTGCAA+AGG + chr8_1:56735314-56735333 None:intergenic 30.0%
!! AACCATTTTCCTTAGCTTTT+GGG + chr8_1:56735439-56735458 None:intergenic 30.0%
!! AAACCATTTTCCTTAGCTTT+TGG + chr8_1:56735440-56735459 None:intergenic 30.0%
! TTTTCTTGAACAATCAAACC+CGG - chr8_1:56735599-56735618 Msa1202990:intron 30.0%
AGTTTACATGAACAGATTCA+CGG + chr8_1:56735726-56735745 None:intergenic 30.0%
ACAACGCAAAAGATATCTAA+TGG + chr8_1:56735903-56735922 None:intergenic 30.0%
! ATTAGATATCTTTTGCGTTG+TGG - chr8_1:56735902-56735921 Msa1202990:intron 30.0%
!! TGTAAAGTTGATATGCAATC+AGG - chr8_1:56735931-56735950 Msa1202990:intron 30.0%
AAGTGAGAAACATCATAATC+TGG + chr8_1:56736076-56736095 None:intergenic 30.0%
! TTGCTGTTAAAATGCAAAAC+TGG - chr8_1:56736236-56736255 Msa1202990:intron 30.0%
AATTTAAACCATGTGACTTG+TGG + chr8_1:56736345-56736364 None:intergenic 30.0%
TGTAAACCATCATATTCAAG+CGG - chr8_1:56736407-56736426 Msa1202990:intron 30.0%
!! AAAAGGTACTCTCAAACTAA+AGG - chr8_1:56736482-56736501 Msa1202990:intron 30.0%
! TAAAGCCGATGGTATTTTAT+AGG - chr8_1:56736526-56736545 Msa1202990:intron 30.0%
TCTGTCCTATAAAATACCAT+CGG + chr8_1:56736534-56736553 None:intergenic 30.0%
!!! CACTAGTTTTATTCATACGT+TGG + chr8_1:56736574-56736593 None:intergenic 30.0%
ATTAGGTATGATTGCATTAG+AGG - chr8_1:56736647-56736666 Msa1202990:intron 30.0%
TTAGGTATGATTGCATTAGA+GGG - chr8_1:56736648-56736667 Msa1202990:intron 30.0%
AAGGAAATTGTCAAGGATAA+TGG + chr8_1:56737005-56737024 None:intergenic 30.0%
CAATTTCCTTAATTTCTTGC+AGG - chr8_1:56737015-56737034 Msa1202990:intron 30.0%
CCAAAAACGCCTAAAATAAA+GGG + chr8_1:56737108-56737127 None:intergenic 30.0%
TCCAAAAACGCCTAAAATAA+AGG + chr8_1:56737109-56737128 None:intergenic 30.0%
CGAGTGTTACAAGTAAAATA+AGG + chr8_1:56737442-56737461 None:intergenic 30.0%
ACTTGTAACACTCGTAAAAT+TGG - chr8_1:56737448-56737467 Msa1202990:five_prime_UTR 30.0%
ATCACATCTCTATACTACTT+TGG + chr8_1:56737584-56737603 None:intergenic 30.0%
GATTTGCAAAAACCGTGAAA+TGG - chr8_1:56733332-56733351 Msa1202990:three_prime_UTR 35.0%
! CTGTTCAACAAGTTTAGAAC+GGG + chr8_1:56733485-56733504 None:intergenic 35.0%
GCTGTTCAACAAGTTTAGAA+CGG + chr8_1:56733486-56733505 None:intergenic 35.0%
CCAAGTAATGATATGAGGTT+TGG + chr8_1:56733566-56733585 None:intergenic 35.0%
CCAAACCTCATATCATTACT+TGG - chr8_1:56733563-56733582 Msa1202990:three_prime_UTR 35.0%
TGAATGTATTCACATGTGTG+AGG - chr8_1:56733785-56733804 Msa1202990:CDS 35.0%
GAATGTATTCACATGTGTGA+GGG - chr8_1:56733786-56733805 Msa1202990:CDS 35.0%
AAGTAGTCACTTCAGATCAA+AGG + chr8_1:56733838-56733857 None:intergenic 35.0%
TATGCCCATTTCAATGTTTC+AGG + chr8_1:56733985-56734004 None:intergenic 35.0%
ATTGAAACCGAGACTTTGTT+TGG - chr8_1:56734041-56734060 Msa1202990:intron 35.0%
! TTGAAACCGAGACTTTGTTT+GGG - chr8_1:56734042-56734061 Msa1202990:intron 35.0%
! TCTTAAAGTGTTTCACCTAG+TGG - chr8_1:56734228-56734247 Msa1202990:intron 35.0%
! CTTAAAGTGTTTCACCTAGT+GGG - chr8_1:56734229-56734248 Msa1202990:intron 35.0%
! CAAACAAAACTTTTCCCACT+AGG + chr8_1:56734246-56734265 None:intergenic 35.0%
AGTCACAAATTATGCCTATG+GGG + chr8_1:56734282-56734301 None:intergenic 35.0%
CACTGCAATTAACTAGTGTT+GGG - chr8_1:56734395-56734414 Msa1202990:intron 35.0%
GATGTAGCACTATATTGTAG+CGG - chr8_1:56734475-56734494 Msa1202990:intron 35.0%
AGAATTTACATGGACAAGGA+AGG + chr8_1:56734796-56734815 None:intergenic 35.0%
GTCAAGAATTTACATGGACA+AGG + chr8_1:56734800-56734819 None:intergenic 35.0%
ATTCATTCACTTTCAAACCC+GGG - chr8_1:56734864-56734883 Msa1202990:intron 35.0%
GCAGAAAACTCAACATAGTA+TGG + chr8_1:56734900-56734919 None:intergenic 35.0%
! TACTATGTTGAGTTTTCTGC+TGG - chr8_1:56734900-56734919 Msa1202990:intron 35.0%
! TTTTCCTGAGATTTAACAGC+AGG + chr8_1:56735097-56735116 None:intergenic 35.0%
! ACATCTGTTGTGAGCTTTAT+AGG - chr8_1:56735140-56735159 Msa1202990:intron 35.0%
!!! GCTGCATTTTTAGGTTAGAA+AGG - chr8_1:56735204-56735223 Msa1202990:intron 35.0%
CTGCAAAGGCTTAAATCAAA+AGG + chr8_1:56735300-56735319 None:intergenic 35.0%
TTTCCAGTAGCAAAACCTTT+GGG + chr8_1:56735371-56735390 None:intergenic 35.0%
!! ACCATTTTCCTTAGCTTTTG+GGG + chr8_1:56735438-56735457 None:intergenic 35.0%
TCAATGCACTTTGCCTTTAT+TGG - chr8_1:56735560-56735579 Msa1202990:intron 35.0%
CAATGCACTTTGCCTTTATT+GGG - chr8_1:56735561-56735580 Msa1202990:intron 35.0%
TTTCTTGAACAATCAAACCC+GGG - chr8_1:56735600-56735619 Msa1202990:intron 35.0%
AAATTAAGACTTGAGTTCCC+CGG + chr8_1:56735621-56735640 None:intergenic 35.0%
AGATATAGTGATAGCATCAG+TGG + chr8_1:56735761-56735780 None:intergenic 35.0%
! CAGTACTGGAGCTATAAAAT+TGG - chr8_1:56735839-56735858 Msa1202990:intron 35.0%
TGGAGCTATAAAATTGGCTA+AGG - chr8_1:56735845-56735864 Msa1202990:intron 35.0%
GGAGCTATAAAATTGGCTAA+GGG - chr8_1:56735846-56735865 Msa1202990:intron 35.0%
!! AAGTTGATATGCAATCAGGT+AGG - chr8_1:56735935-56735954 Msa1202990:intron 35.0%
! AGTTGATATGCAATCAGGTA+GGG - chr8_1:56735936-56735955 Msa1202990:intron 35.0%
! TAGGGTGTTCTGTATTCTAT+TGG - chr8_1:56735954-56735973 Msa1202990:intron 35.0%
! AGGGTGTTCTGTATTCTATT+GGG - chr8_1:56735955-56735974 Msa1202990:intron 35.0%
TGCAATAAGATCTTGCACAA+GGG + chr8_1:56736153-56736172 None:intergenic 35.0%
AAAACTGGATTTCTGATTCC+AGG - chr8_1:56736251-56736270 Msa1202990:intron 35.0%
TCTACCATCATTTCGTACAA+TGG + chr8_1:56736376-56736395 None:intergenic 35.0%
AAATGATGGTAGAATGAGTG+AGG - chr8_1:56736383-56736402 Msa1202990:intron 35.0%
GTAAACCATCATATTCAAGC+GGG - chr8_1:56736408-56736427 Msa1202990:intron 35.0%
TAAACCATCATATTCAAGCG+GGG - chr8_1:56736409-56736428 Msa1202990:intron 35.0%
GTGGTTGAAAATTGAAATGG+AGG - chr8_1:56736431-56736450 Msa1202990:intron 35.0%
GTTGAAAATTGAAATGGAGG+AGG - chr8_1:56736434-56736453 Msa1202990:intron 35.0%
CACTGAATGTGTAGAAATAC+TGG - chr8_1:56736614-56736633 Msa1202990:intron 35.0%
GAATGTGTAGAAATACTGGA+CGG - chr8_1:56736618-56736637 Msa1202990:intron 35.0%
AATGTGTAGAAATACTGGAC+GGG - chr8_1:56736619-56736638 Msa1202990:intron 35.0%
ATCAACCCTGCAAGAAATTA+AGG + chr8_1:56737024-56737043 None:intergenic 35.0%
!!! CCCTTTATTTTAGGCGTTTT+TGG - chr8_1:56737105-56737124 Msa1202990:intron 35.0%
AAGAATGCTCATATGGACAA+TGG + chr8_1:56737224-56737243 None:intergenic 35.0%
TGACAGCACTCAAATATTCA+TGG + chr8_1:56737319-56737338 None:intergenic 35.0%
!! TTCACTTAGCTTGTGCATAT+GGG + chr8_1:56737409-56737428 None:intergenic 35.0%
!! ATTCACTTAGCTTGTGCATA+TGG + chr8_1:56737410-56737429 None:intergenic 35.0%
GAAATTTCATGCTTCTCTGT+AGG + chr8_1:56737475-56737494 None:intergenic 35.0%
ATTTATGCTGAGATCGGATC+TGG + chr8_1:56733519-56733538 None:intergenic 40.0%
TTCAATGACTGACTAATCCG+AGG - chr8_1:56733893-56733912 Msa1202990:intron 40.0%
TCAATGACTGACTAATCCGA+GGG - chr8_1:56733894-56733913 Msa1202990:intron 40.0%
TCGGACCTGAAACATTGAAA+TGG - chr8_1:56733977-56733996 Msa1202990:intron 40.0%
CGGACCTGAAACATTGAAAT+GGG - chr8_1:56733978-56733997 Msa1202990:intron 40.0%
ATTGAAATGGGCATACTCCA+AGG - chr8_1:56733990-56734009 Msa1202990:intron 40.0%
GTAAGTCCCAAACAAAGTCT+CGG + chr8_1:56734051-56734070 None:intergenic 40.0%
TAATGAAATGCCACCTGCTA+AGG - chr8_1:56734173-56734192 Msa1202990:intron 40.0%
TAAGATTCATCCTTAGCAGG+TGG + chr8_1:56734186-56734205 None:intergenic 40.0%
TGGTAAGATTCATCCTTAGC+AGG + chr8_1:56734189-56734208 None:intergenic 40.0%
ACATCTCTCATTCTTTCAGC+TGG + chr8_1:56734209-56734228 None:intergenic 40.0%
!! GTTTAAAAGGGTATCCCCAT+AGG - chr8_1:56734265-56734284 Msa1202990:intron 40.0%
TGGAAGTAGATCGTGATCTT+AGG + chr8_1:56734377-56734396 None:intergenic 40.0%
CCAACACTAGTTAATTGCAG+TGG + chr8_1:56734397-56734416 None:intergenic 40.0%
CCACTGCAATTAACTAGTGT+TGG - chr8_1:56734394-56734413 Msa1202990:intron 40.0%
! TCCTCAAATTACGGCTTTAG+TGG - chr8_1:56734548-56734567 Msa1202990:intron 40.0%
ACCACTAAAGCCGTAATTTG+AGG + chr8_1:56734552-56734571 None:intergenic 40.0%
! GTAGGTAGGTGTTATGCTTA+TGG + chr8_1:56734938-56734957 None:intergenic 40.0%
ATCCTCGAAATAGTGTAGGT+AGG + chr8_1:56734952-56734971 None:intergenic 40.0%
CTACATCCTCGAAATAGTGT+AGG + chr8_1:56734956-56734975 None:intergenic 40.0%
GGATGTAGTCGAAATTATGC+TGG - chr8_1:56734968-56734987 Msa1202990:intron 40.0%
TTTAGGCCTCTTGTGCTTTA+AGG - chr8_1:56735035-56735054 Msa1202990:intron 40.0%
CCATAACCTTAAAGCACAAG+AGG + chr8_1:56735044-56735063 None:intergenic 40.0%
!! CCTCTTGTGCTTTAAGGTTA+TGG - chr8_1:56735041-56735060 Msa1202990:intron 40.0%
TGCCCCCAATCCTAAATATA+TGG - chr8_1:56735234-56735253 Msa1202990:intron 40.0%
! AAATGGGAAAGTCTGGAACT+AGG + chr8_1:56735275-56735294 None:intergenic 40.0%
AAAGGGTAAATGGGAAAGTC+TGG + chr8_1:56735282-56735301 None:intergenic 40.0%
CTTTCCAGTAGCAAAACCTT+TGG + chr8_1:56735372-56735391 None:intergenic 40.0%
!! CCATTTTCCTTAGCTTTTGG+GGG + chr8_1:56735437-56735456 None:intergenic 40.0%
CTTTGCCTTTATTGGGATCA+CGG - chr8_1:56735568-56735587 Msa1202990:intron 40.0%
TTCTTGAACAATCAAACCCG+GGG - chr8_1:56735601-56735620 Msa1202990:intron 40.0%
AATTAAGACTTGAGTTCCCC+GGG + chr8_1:56735620-56735639 None:intergenic 40.0%
TTAAGCTGTGGTCCAATCAA+TGG + chr8_1:56735811-56735830 None:intergenic 40.0%
! TTTTATAGCTCCAGTACTGC+CGG + chr8_1:56735838-56735857 None:intergenic 40.0%
TGTTCCATGTTAGTTGACCA+AGG - chr8_1:56736104-56736123 Msa1202990:intron 40.0%
TGAACCTTGGTCAACTAACA+TGG + chr8_1:56736111-56736130 None:intergenic 40.0%
GTGCAATAAGATCTTGCACA+AGG + chr8_1:56736154-56736173 None:intergenic 40.0%
ATTCCAGGCAGGATTTATGT+AGG - chr8_1:56736266-56736285 Msa1202990:intron 40.0%
CAGGATTTATGTAGGTCATG+TGG - chr8_1:56736274-56736293 Msa1202990:intron 40.0%
AAGCTTCGATAGCTTAGACA+TGG - chr8_1:56736299-56736318 Msa1202990:intron 40.0%
GATAGCTTAGACATGGATGT+TGG - chr8_1:56736306-56736325 Msa1202990:intron 40.0%
! ATGTGACTTGTGGTATGACA+TGG + chr8_1:56736335-56736354 None:intergenic 40.0%
ATGTCATACCACAAGTCACA+TGG - chr8_1:56736334-56736353 Msa1202990:intron 40.0%
AGGTCCATTGTACGAAATGA+TGG - chr8_1:56736369-56736388 Msa1202990:intron 40.0%
GGGGTGGTTGAAAATTGAAA+TGG - chr8_1:56736428-56736447 Msa1202990:intron 40.0%
TTATAGGACAGATTGTTGGG+TGG - chr8_1:56736542-56736561 Msa1202990:intron 40.0%
! TTAGATGGTTTGTGCGTATG+TGG - chr8_1:56736709-56736728 Msa1202990:intron 40.0%
AAGGAAAGTAGATCAGACGA+AGG - chr8_1:56736755-56736774 Msa1202990:intron 40.0%
AAAGTAGATCAGACGAAGGA+TGG - chr8_1:56736759-56736778 Msa1202990:intron 40.0%
CTTATTCCACTAGGTCTACA+TGG + chr8_1:56736898-56736917 None:intergenic 40.0%
CATGTAGACCTAGTGGAATA+AGG - chr8_1:56736896-56736915 Msa1202990:intron 40.0%
! ATGGGTTCTGTTGTTGTTGT+GGG - chr8_1:56736920-56736939 Msa1202990:intron 40.0%
ATAAAGGGTCAAGCAGGATA+TGG + chr8_1:56737093-56737112 None:intergenic 40.0%
CCTAAAATAAAGGGTCAAGC+AGG + chr8_1:56737099-56737118 None:intergenic 40.0%
!!! CCTGCTTGACCCTTTATTTT+AGG - chr8_1:56737096-56737115 Msa1202990:intron 40.0%
GTCCATATGAGCATTCTTTG+TGG - chr8_1:56737226-56737245 Msa1202990:intron 40.0%
CACCACAAAGAATGCTCATA+TGG + chr8_1:56737231-56737250 None:intergenic 40.0%
TCTTTGTGGTGATATCTCAC+AGG - chr8_1:56737240-56737259 Msa1202990:intron 40.0%
ATATCTCACAGGATGTACAC+TGG - chr8_1:56737251-56737270 Msa1202990:intron 40.0%
AGTGCTGTCATTACTAGTGT+AGG - chr8_1:56737329-56737348 Msa1202990:intron 40.0%
CCATCAACACTCAAAAACGT+AGG + chr8_1:56737371-56737390 None:intergenic 40.0%
!!! CCTACGTTTTTGAGTGTTGA+TGG - chr8_1:56737368-56737387 Msa1202990:CDS 40.0%
! TGAGGAATTTCCTTTTTCCC+AGG - chr8_1:56737521-56737540 Msa1202990:five_prime_UTR 40.0%
!! TGCTGTGACATTTCTAGCAT+AGG - chr8_1:56737550-56737569 Msa1202990:five_prime_UTR 40.0%
! GATCTGAGACAAACACGGTT+TGG - chr8_1:56733392-56733411 Msa1202990:three_prime_UTR 45.0%
TTGGGAGAGAAGAAAGTGAG+AGG + chr8_1:56733547-56733566 None:intergenic 45.0%
! ATTCACATGTGTGAGGGTGT+TGG - chr8_1:56733792-56733811 Msa1202990:CDS 45.0%
! CAAAGGCGTGTCAAACTATC+TGG + chr8_1:56733821-56733840 None:intergenic 45.0%
CAATGACTGACTAATCCGAG+GGG - chr8_1:56733895-56733914 Msa1202990:intron 45.0%
AATGACTGACTAATCCGAGG+GGG - chr8_1:56733896-56733915 Msa1202990:intron 45.0%
TAGATTTAAATGGGCCCCCT+CGG + chr8_1:56733913-56733932 None:intergenic 45.0%
CTAGAGAGCAAAGCTCTGTA+AGG - chr8_1:56733930-56733949 Msa1202990:intron 45.0%
!! CTCTGTAAGGACTAGCCTAT+TGG - chr8_1:56733943-56733962 Msa1202990:intron 45.0%
GTTTCAATTCAACTCCGTCG+TGG + chr8_1:56734029-56734048 None:intergenic 45.0%
TGCAAGACTAACCTTGAGAC+TGG - chr8_1:56734303-56734322 Msa1202990:intron 45.0%
GCAAGACTAACCTTGAGACT+GGG - chr8_1:56734304-56734323 Msa1202990:intron 45.0%
AGTCCACCATCAAATTCTGG+GGG + chr8_1:56734773-56734792 None:intergenic 45.0%
GAGTCCACCATCAAATTCTG+GGG + chr8_1:56734774-56734793 None:intergenic 45.0%
GGAGTCCACCATCAAATTCT+GGG + chr8_1:56734775-56734794 None:intergenic 45.0%
AGGAGTCCACCATCAAATTC+TGG + chr8_1:56734776-56734795 None:intergenic 45.0%
TAGTATGGCATTCAGATGCC+CGG + chr8_1:56734885-56734904 None:intergenic 45.0%
CACCTACCTACACTATTTCG+AGG - chr8_1:56734947-56734966 Msa1202990:intron 45.0%
GACACCTGCTGTTAAATCTC+AGG - chr8_1:56735090-56735109 Msa1202990:intron 45.0%
! GGTGTGACAGCTGCATTTTT+AGG - chr8_1:56735195-56735214 Msa1202990:intron 45.0%
! TAGCCCAAAGGTTTTGCTAC+TGG - chr8_1:56735365-56735384 Msa1202990:intron 45.0%
ATCGAAACCCCCAAAAGCTA+AGG - chr8_1:56735427-56735446 Msa1202990:intron 45.0%
CCCCCAAAAGCTAAGGAAAA+TGG - chr8_1:56735434-56735453 Msa1202990:intron 45.0%
GAGATCCGTGATCCCAATAA+AGG + chr8_1:56735576-56735595 None:intergenic 45.0%
TTTATAGCTCCAGTACTGCC+GGG + chr8_1:56735837-56735856 None:intergenic 45.0%
TTGACCAAGGTTCATAACCC+TGG - chr8_1:56736117-56736136 Msa1202990:intron 45.0%
TGACCTACATAAATCCTGCC+TGG + chr8_1:56736272-56736291 None:intergenic 45.0%
ATTGAAATGGAGGAGGCTCT+TGG - chr8_1:56736441-56736460 Msa1202990:intron 45.0%
TTGAAATGGAGGAGGCTCTT+GGG - chr8_1:56736442-56736461 Msa1202990:intron 45.0%
!! CCATCGGCTTTAGTGTTGTA+AGG + chr8_1:56736518-56736537 None:intergenic 45.0%
CCTTACAACACTAAAGCCGA+TGG - chr8_1:56736515-56736534 Msa1202990:intron 45.0%
ATACTGGACGGGATGAGATT+AGG - chr8_1:56736630-56736649 Msa1202990:intron 45.0%
GTTGATCCATGTAGACCTAG+TGG - chr8_1:56736889-56736908 Msa1202990:intron 45.0%
AGACCTAGTGGAATAAGGCA+TGG - chr8_1:56736901-56736920 Msa1202990:intron 45.0%
GACCTAGTGGAATAAGGCAT+GGG - chr8_1:56736902-56736921 Msa1202990:intron 45.0%
! AACCCATGCCTTATTCCACT+AGG + chr8_1:56736907-56736926 None:intergenic 45.0%
! CATGGGTTCTGTTGTTGTTG+TGG - chr8_1:56736919-56736938 Msa1202990:intron 45.0%
TCATACGCCTAAGAGCTTCT+AGG - chr8_1:56737165-56737184 Msa1202990:intron 45.0%
AGGTTTGCCTAGAAGCTCTT+AGG + chr8_1:56737175-56737194 None:intergenic 45.0%
AAGAGCTTCTAGGCAAACCT+TGG - chr8_1:56737175-56737194 Msa1202990:intron 45.0%
AGCAGAAGATCCTGGGAAAA+AGG + chr8_1:56737534-56737553 None:intergenic 45.0%
ATGTCACAGCAGAAGATCCT+GGG + chr8_1:56737541-56737560 None:intergenic 45.0%
AATGTCACAGCAGAAGATCC+TGG + chr8_1:56737542-56737561 None:intergenic 45.0%
!!! CATTTTTTTAAAAAATATTT+GGG - chr8_1:56735485-56735504 Msa1202990:intron 5.0%
!!! ATTTTTTTAAAAAATATTTG+GGG - chr8_1:56735486-56735505 Msa1202990:intron 5.0%
!! ATGAAGCACTGACACAGACG+CGG - chr8_1:56733723-56733742 Msa1202990:CDS 50.0%
!! AAGGACTAGCCTATTGGCAC+TGG - chr8_1:56733949-56733968 Msa1202990:intron 50.0%
! CCTATTGGCACTGGAGGAAT+CGG - chr8_1:56733958-56733977 Msa1202990:intron 50.0%
ATTCAACTCCGTCGTGGTGT+TGG + chr8_1:56734023-56734042 None:intergenic 50.0%
CTAACCTTGAGACTGGGTAG+TGG - chr8_1:56734310-56734329 Msa1202990:intron 50.0%
CTAACCACTACCCAGTCTCA+AGG + chr8_1:56734317-56734336 None:intergenic 50.0%
! CTTGAGACTGGGTAGTGGTT+AGG - chr8_1:56734315-56734334 Msa1202990:intron 50.0%
!! TTAGTGGTGCCATAGCACTG+CGG - chr8_1:56734564-56734583 Msa1202990:intron 50.0%
!! GGGGGTATCGAAAACTAGCT+GGG + chr8_1:56734754-56734773 None:intergenic 50.0%
CGATACCCCCCAGAATTTGA+TGG - chr8_1:56734764-56734783 Msa1202990:intron 50.0%
TACCCCCCAGAATTTGATGG+TGG - chr8_1:56734767-56734786 Msa1202990:intron 50.0%
GTCCACCATCAAATTCTGGG+GGG + chr8_1:56734772-56734791 None:intergenic 50.0%
AGTATGGCATTCAGATGCCC+GGG + chr8_1:56734884-56734903 None:intergenic 50.0%
! GGGAAAGTCTGGAACTAGGA+CGG + chr8_1:56735271-56735290 None:intergenic 50.0%
CTGGATTTCTGATTCCAGGC+AGG - chr8_1:56736255-56736274 Msa1202990:intron 50.0%
ACCATCATATTCAAGCGGGG+TGG - chr8_1:56736412-56736431 Msa1202990:intron 50.0%
ACCACCCCGCTTGAATATGA+TGG + chr8_1:56736416-56736435 None:intergenic 50.0%
! GATGGTTTGTGCGTATGTGG+AGG - chr8_1:56736712-56736731 Msa1202990:intron 50.0%
! GCTTTATCGCTAGAAGCACG+AGG - chr8_1:56736781-56736800 Msa1202990:intron 50.0%
!! TTGCAGGGTTGATGTTGCTG+TGG - chr8_1:56737031-56737050 Msa1202990:intron 50.0%
CTTCTAGGCAAACCTTGGCA+AGG - chr8_1:56737180-56737199 Msa1202990:intron 50.0%
GCAAACCTTGGCAAGGAAGA+GGG - chr8_1:56737187-56737206 Msa1202990:intron 50.0%
TCTTTCCCTCTTCCTTGCCA+AGG + chr8_1:56737195-56737214 None:intergenic 50.0%
GGATGTACACTGGCAGACAA+AGG - chr8_1:56737261-56737280 Msa1202990:intron 50.0%
!! GACTAGCCTATTGGCACTGG+AGG - chr8_1:56733952-56733971 Msa1202990:intron 55.0%
CCGATTCCTCCAGTGCCAAT+AGG + chr8_1:56733961-56733980 None:intergenic 55.0%
GTGCTACATCGCTGCTGTTG+TGG + chr8_1:56734465-56734484 None:intergenic 55.0%
TCTGCTACACCGCAGTGCTA+TGG + chr8_1:56734576-56734595 None:intergenic 55.0%
!! GGGGGGTATCGAAAACTAGC+TGG + chr8_1:56734755-56734774 None:intergenic 55.0%
TGGACCACAGCTTAAGAGCC+CGG - chr8_1:56735816-56735835 Msa1202990:intron 55.0%
CCTGCCAGGGTTATGAACCT+TGG + chr8_1:56736124-56736143 None:intergenic 55.0%
CCAAGGTTCATAACCCTGGC+AGG - chr8_1:56736121-56736140 Msa1202990:intron 55.0%
GGCAAACCTTGGCAAGGAAG+AGG - chr8_1:56737186-56737205 Msa1202990:intron 55.0%
!! GTGGTGTTGGCTTGGGACCT+TGG + chr8_1:56734010-56734029 None:intergenic 60.0%
GTCCCAAGCCAACACCACGA+CGG - chr8_1:56734012-56734031 Msa1202990:intron 60.0%
!! CTCCGTCGTGGTGTTGGCTT+GGG + chr8_1:56734017-56734036 None:intergenic 60.0%
!! ACTCCGTCGTGGTGTTGGCT+TGG + chr8_1:56734018-56734037 None:intergenic 60.0%
ACTGCCGGGCTCTTAAGCTG+TGG + chr8_1:56735823-56735842 None:intergenic 60.0%
GCTTAAGAGCCCGGCAGTAC+TGG - chr8_1:56735825-56735844 Msa1202990:intron 60.0%
GGTTCATAACCCTGGCAGGC+TGG - chr8_1:56736125-56736144 Msa1202990:intron 60.0%
ACAAGGGAGCCAGCCTGCCA+GGG + chr8_1:56736137-56736156 None:intergenic 65.0%
CACAAGGGAGCCAGCCTGCC+AGG + chr8_1:56736138-56736157 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 56733246 56737669 56733246 ID=Msa1202990;Name=Msa1202990
chr8_1 mRNA 56733246 56737669 56733246 ID=Msa1202990-mRNA-1;Parent=Msa1202990;Name=Msa1202990-mRNA-1;_AED=0.00;_eAED=0.00;_QI=229|1|1|1|0|0.5|2|434|102
chr8_1 exon 56733246 56733878 56733246 ID=Msa1202990-mRNA-1:exon:18519;Parent=Msa1202990-mRNA-1
chr8_1 exon 56737331 56737669 56737331 ID=Msa1202990-mRNA-1:exon:18518;Parent=Msa1202990-mRNA-1
chr8_1 five_prime_UTR 56737441 56737669 56737441 ID=Msa1202990-mRNA-1:five_prime_utr;Parent=Msa1202990-mRNA-1
chr8_1 CDS 56737331 56737440 56737331 ID=Msa1202990-mRNA-1:cds;Parent=Msa1202990-mRNA-1
chr8_1 CDS 56733680 56733878 56733680 ID=Msa1202990-mRNA-1:cds;Parent=Msa1202990-mRNA-1
chr8_1 three_prime_UTR 56733246 56733679 56733246 ID=Msa1202990-mRNA-1:three_prime_utr;Parent=Msa1202990-mRNA-1
Gene Sequence

>Msa1202990

ATGATAACCCGTTCTAAACTTGTTGAACAGCTCAGAGATTACCAGATCCGATCTCAGCATAAATATTCTCCTCTCACTTTCTTCTCTCCCAAACCTCATATCATTACTTGGGTTGATGTTGCTGTGGCAATTGCTTTTGCTCTAGTGTTCTGCATGCTTGTCGCCATATCCTGCTTGACCCTTTATTTTAGGCGTTTTTGGATTTTTCTTCTTGTTGTAAGCTTCAGTATAATTCTTCTCATACGCCTAAGAGCTTCTAGGCAAACCTTGGCAAGGAAGAGGGAAAGAAGATTGCCATTGTCCATATGA
Protein sequence

>Msa1202990

MITRSKLVEQLRDYQIRSQHKYSPLTFFSPKPHIITWVDVAVAIAFALVFCMLVAISCLTLYFRRFWIFLLVVSFSIILLIRLRASRQTLARKRERRLPLSI*