AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1443830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1443830 MtrunA17_Chr8g0373451 96.078 102 4 0 1 102 1 102 2.63e-65 192
Msa1443830 MtrunA17_Chr3g0143691 42.593 108 56 3 1 102 1 108 1.15e-15 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1443830 AT1G20460.1 42.453 106 57 3 1 102 1 106 1.14e-14 64.3
Msa1443830 AT1G76185.1 42.453 106 57 3 1 102 1 106 4.57e-12 57.8

Find 44 sgRNAs with CRISPR-Local

Find 324 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTTGTTAGTTAGTTAAAA+AGG 0.180464 tig0030547:-11502 Msa1443830:five_prime_UTR
CGAGTGTTACAAGTAAAATA+AGG 0.239823 tig0030547:+7501 None:intergenic
TATATCTTACATAATTAATT+TGG 0.249603 tig0030547:-7651 Msa1443830:three_prime_UTR
ACTTGTAACACTCGTAAAAT+TGG 0.284676 tig0030547:-7492 Msa1443830:three_prime_UTR
ATTCACTTAGCTTGTGCATA+TGG 0.310277 tig0030547:+7533 None:intergenic
AGCAGAAGATCCTGGGAAAA+AGG 0.316556 tig0030547:+7409 None:intergenic
TCATACGCCTAAGAGCTTCT+AGG 0.326975 tig0030547:-7775 Msa1443830:CDS
CCAAGTAATGATATGAGGTT+TGG 0.331279 tig0030547:+11377 None:intergenic
ATAAAGGGTCAAGCAGGATA+TGG 0.382041 tig0030547:+7850 None:intergenic
CTGTTCAACAAGTTTAGAAC+GGG 0.430395 tig0030547:+11458 None:intergenic
GGCAAACCTTGGCAAGGAAG+AGG 0.430808 tig0030547:-7754 Msa1443830:CDS
AGGTTTGCCTAGAAGCTCTT+AGG 0.436059 tig0030547:+7768 None:intergenic
GATCTGAGACAAACACGGTT+TGG 0.440546 tig0030547:-11548 Msa1443830:five_prime_UTR
ACATAAACACAACCATTTCA+CGG 0.443810 tig0030547:+11596 None:intergenic
CAAGTAATGATATGAGGTTT+GGG 0.444710 tig0030547:+11378 None:intergenic
TTCACTTAGCTTGTGCATAT+GGG 0.450193 tig0030547:+7534 None:intergenic
TCTTTCCCTCTTCCTTGCCA+AGG 0.451193 tig0030547:+7748 None:intergenic
CACCACAAAGAATGCTCATA+TGG 0.453210 tig0030547:+7712 None:intergenic
CCAAACCTCATATCATTACT+TGG 0.454335 tig0030547:-11377 Msa1443830:intron
TGACAGCACTCAAATATTCA+TGG 0.456413 tig0030547:+7624 None:intergenic
AATGTCACAGCAGAAGATCC+TGG 0.464356 tig0030547:+7401 None:intergenic
ATTTATGCTGAGATCGGATC+TGG 0.468524 tig0030547:+11424 None:intergenic
ATCACATCTCTATACTACTT+TGG 0.480081 tig0030547:+7359 None:intergenic
TGCTGTGACATTTCTAGCAT+AGG 0.492979 tig0030547:-7390 Msa1443830:three_prime_UTR
GAGTGTTACAAGTAAAATAA+GGG 0.495682 tig0030547:+7502 None:intergenic
AATTACCAAGTAATGATATG+AGG 0.499125 tig0030547:+11372 None:intergenic
GTCCATATGAGCATTCTTTG+TGG 0.501670 tig0030547:-7714 Msa1443830:exon
GAGAATATTTATGCTGAGAT+CGG 0.512331 tig0030547:+11418 None:intergenic
GAAATTTCATGCTTCTCTGT+AGG 0.523780 tig0030547:+7468 None:intergenic
CCTAAAATAAAGGGTCAAGC+AGG 0.550751 tig0030547:+7844 None:intergenic
AGTGCTGTCATTACTAGTGT+AGG 0.555939 tig0030547:-7611 Msa1443830:three_prime_UTR
ATGTCACAGCAGAAGATCCT+GGG 0.557119 tig0030547:+7402 None:intergenic
GCAAACCTTGGCAAGGAAGA+GGG 0.560246 tig0030547:-7753 Msa1443830:CDS
TTGGGAGAGAAGAAAGTGAG+AGG 0.560855 tig0030547:+11396 None:intergenic
TTAATGAATGAATGAAGAAG+AGG 0.576063 tig0030547:+11659 None:intergenic
TTGCAGGGTTGATGTTGCTG+TGG 0.577557 tig0030547:-7909 Msa1443830:intron
TCTTTGTGGTGATATCTCAC+AGG 0.583891 tig0030547:-7700 Msa1443830:three_prime_UTR
AAGAGCTTCTAGGCAAACCT+TGG 0.584867 tig0030547:-7765 Msa1443830:CDS
GCTGTTCAACAAGTTTAGAA+CGG 0.592461 tig0030547:+11457 None:intergenic
CTTCTAGGCAAACCTTGGCA+AGG 0.624301 tig0030547:-7760 Msa1443830:CDS
AAGAATGCTCATATGGACAA+TGG 0.637377 tig0030547:+7719 None:intergenic
GGATGTACACTGGCAGACAA+AGG 0.645230 tig0030547:-7679 Msa1443830:three_prime_UTR
ATATCTCACAGGATGTACAC+TGG 0.678712 tig0030547:-7689 Msa1443830:three_prime_UTR
TATATGATCTGAGACAAACA+CGG 0.765222 tig0030547:-11553 Msa1443830:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTATTTTTTTAAAAAACT+TGG - tig0030547:7910-7929 Msa1443830:intron 10.0%
!! TATATATAATATGTCAAAAT+GGG + tig0030547:9058-9077 None:intergenic 10.0%
!! ATATATATAATATGTCAAAA+TGG + tig0030547:9059-9078 None:intergenic 10.0%
!!! CATATTATATATATGTTTTT+AGG - tig0030547:9065-9084 Msa1443830:intron 10.0%
!!! CCAAATATTTTTTAAAAAAA+TGG + tig0030547:9534-9553 None:intergenic 10.0%
!!! CCATTTTTTTAAAAAATATT+TGG - tig0030547:9531-9550 Msa1443830:intron 10.0%
!! AATTCATACAAATATATAAA+AGG - tig0030547:10230-10249 Msa1443830:intron 10.0%
!! TATATCTTACATAATTAATT+TGG - tig0030547:11336-11355 Msa1443830:intron 10.0%
!!! CTATTTTTAAAAGAAAAACA+GGG + tig0030547:8165-8184 None:intergenic 15.0%
!!! AAAAGTTTTGTTTGTTTAAA+AGG - tig0030547:8299-8318 Msa1443830:intron 15.0%
!!! AAAGTTTTGTTTGTTTAAAA+GGG - tig0030547:8300-8319 Msa1443830:intron 15.0%
!! ATATATAATATGTCAAAATG+GGG + tig0030547:9057-9076 None:intergenic 15.0%
!! AAATTGAATTCCATATATTT+AGG + tig0030547:9294-9313 None:intergenic 15.0%
!!! TTTAACCATATGAAATTTTA+AGG - tig0030547:9367-9386 Msa1443830:intron 15.0%
!!! TCATTGTATTTAATTTAACA+GGG + tig0030547:9703-9722 None:intergenic 15.0%
!! ATATATTTGTATGAATTCAT+TGG + tig0030547:10227-10246 None:intergenic 15.0%
!!! TGTATTTTGATATGATAAAA+AGG - tig0030547:10512-10531 Msa1443830:intron 15.0%
!! AGTTTCTTCTATAATTTATT+TGG + tig0030547:10856-10875 None:intergenic 15.0%
!! AAGTTGTTAGTTAGTTAAAA+AGG - tig0030547:7485-7504 Msa1443830:three_prime_UTR 20.0%
!! AGTTGTTAGTTAGTTAAAAA+GGG - tig0030547:7486-7505 Msa1443830:three_prime_UTR 20.0%
!! GTAAAAAAAAAAAAAGTGAG+AGG + tig0030547:7647-7666 None:intergenic 20.0%
!!! TTTTTAAAAAACTTGGTATC+TGG - tig0030547:7917-7936 Msa1443830:intron 20.0%
!!! CCTATTTTTAAAAGAAAAAC+AGG + tig0030547:8166-8185 None:intergenic 20.0%
!!! CCTGTTTTTCTTTTAAAAAT+AGG - tig0030547:8163-8182 Msa1443830:intron 20.0%
!! TATCTAATAGGTAATAAATG+AGG - tig0030547:9217-9236 Msa1443830:intron 20.0%
!!! AATTCCATATATTTAGGATT+GGG + tig0030547:9288-9307 None:intergenic 20.0%
!!! TTTTGCCTTAAAATTTCATA+TGG + tig0030547:9375-9394 None:intergenic 20.0%
!!! TAGTATTGAAAATTTGAAAG+CGG + tig0030547:9451-9470 None:intergenic 20.0%
!!! GTCATTGTATTTAATTTAAC+AGG + tig0030547:9704-9723 None:intergenic 20.0%
!! TTCCTTACTATAGAATATAT+AGG + tig0030547:10788-10807 None:intergenic 20.0%
!!! TGATTTTTGATCAAATACTA+TGG - tig0030547:10909-10928 Msa1443830:intron 20.0%
!!! TATTGTAGATTTTTACAATG+AGG - tig0030547:11550-11569 Msa1443830:five_prime_UTR 20.0%
!!! AAAACTGGGTTTTGATTTTT+AGG - tig0030547:7296-7315 Msa1443830:three_prime_UTR 25.0%
!!! AAACTGGGTTTTGATTTTTA+GGG - tig0030547:7297-7316 Msa1443830:three_prime_UTR 25.0%
! TTAATGAATGAATGAAGAAG+AGG + tig0030547:7331-7350 None:intergenic 25.0%
! AATTACCAAGTAATGATATG+AGG + tig0030547:7618-7637 None:intergenic 25.0%
! TTTGCTCTCTAGATTTAAAT+GGG + tig0030547:7969-7988 None:intergenic 25.0%
! TACAAAATGTCCTCAAATTA+CGG - tig0030547:8586-8605 Msa1443830:intron 25.0%
!!! AAGATTGATAGTTTTTCGAT+TGG - tig0030547:8731-8750 Msa1443830:intron 25.0%
!!! AGATTGATAGTTTTTCGATT+GGG - tig0030547:8732-8751 Msa1443830:intron 25.0%
! ATGTAAATTCTTGACATTGA+GGG - tig0030547:8852-8871 Msa1443830:intron 25.0%
!!! ATTCAGGTTGCATATAAATA+TGG - tig0030547:9115-9134 Msa1443830:intron 25.0%
!! ATAGGTTTTCGTTATCTAAT+AGG - tig0030547:9205-9224 Msa1443830:intron 25.0%
!! ATTCCATATATTTAGGATTG+GGG + tig0030547:9287-9306 None:intergenic 25.0%
!! GAATTCCATATATTTAGGAT+TGG + tig0030547:9289-9308 None:intergenic 25.0%
! CTTAAATCAAAAGGGTAAAT+GGG + tig0030547:9338-9357 None:intergenic 25.0%
! AAAATGAAATATTAGCCCAA+AGG - tig0030547:9400-9419 Msa1443830:intron 25.0%
!!! GGTTTGTCTTTGAAATAAAA+TGG - tig0030547:9502-9521 Msa1443830:intron 25.0%
! GTTTGATTGTTCAAGAAAAA+TGG + tig0030547:9645-9664 None:intergenic 25.0%
!! TTTAATTTAACAGGGAAGAA+GGG + tig0030547:9695-9714 None:intergenic 25.0%
!! ATTTAATTTAACAGGGAAGA+AGG + tig0030547:9696-9715 None:intergenic 25.0%
! ACTATAAGTTAGCATATTTG+AGG + tig0030547:9734-9753 None:intergenic 25.0%
! TAAAGTACAAAACCATTGAT+TGG - tig0030547:9843-9862 Msa1443830:intron 25.0%
! GGATGTAAAAATTTATGACT+CGG - tig0030547:10023-10042 Msa1443830:intron 25.0%
! GATGTAAAAATTTATGACTC+GGG - tig0030547:10024-10043 Msa1443830:intron 25.0%
! TCACATGGTTTAAATTTCTT+AGG - tig0030547:10396-10415 Msa1443830:intron 25.0%
!! TATTTTATAGGACAGATTGT+TGG - tig0030547:10585-10604 Msa1443830:intron 25.0%
!! ATTTTATAGGACAGATTGTT+GGG - tig0030547:10586-10605 Msa1443830:intron 25.0%
!! TTATCTGTCTTTTCTACAAT+AGG + tig0030547:10728-10747 None:intergenic 25.0%
! CAGATAAATCTTGATTTAGA+TGG - tig0030547:10741-10760 Msa1443830:intron 25.0%
! GACCTATATATTCTATAGTA+AGG - tig0030547:10783-10802 Msa1443830:intron 25.0%
! CGTATAAAATACATTGAAGA+TGG - tig0030547:11008-11027 Msa1443830:intron 25.0%
! AAAATACATTGAAGATGGTT+AGG - tig0030547:11013-11032 Msa1443830:intron 25.0%
! AGAAATTAAGGAAATTGTCA+AGG + tig0030547:11059-11078 None:intergenic 25.0%
! AATTTCCTTAATTTCTTGCA+GGG - tig0030547:11063-11082 Msa1443830:intron 25.0%
! GAGTGTTACAAGTAAAATAA+GGG + tig0030547:11488-11507 None:intergenic 25.0%
!!! AACTGGGTTTTGATTTTTAG+GGG - tig0030547:7298-7317 Msa1443830:three_prime_UTR 30.0%
!! ACTGTATTTTTACGATTTGG+CGG + tig0030547:7355-7374 None:intergenic 30.0%
!! GAGACTGTATTTTTACGATT+TGG + tig0030547:7358-7377 None:intergenic 30.0%
ACATAAACACAACCATTTCA+CGG + tig0030547:7394-7413 None:intergenic 30.0%
! TATATGATCTGAGACAAACA+CGG - tig0030547:7434-7453 Msa1443830:three_prime_UTR 30.0%
GAGAATATTTATGCTGAGAT+CGG + tig0030547:7572-7591 None:intergenic 30.0%
CAAGTAATGATATGAGGTTT+GGG + tig0030547:7612-7631 None:intergenic 30.0%
! TCTAAAACAACTACTTGTGT+CGG + tig0030547:7799-7818 None:intergenic 30.0%
CTTTGCTCTCTAGATTTAAA+TGG + tig0030547:7970-7989 None:intergenic 30.0%
ACTTCGTATATTGAAAGTGA+TGG - tig0030547:8116-8135 Msa1443830:intron 30.0%
TAGTCACAAATTATGCCTAT+GGG + tig0030547:8330-8349 None:intergenic 30.0%
ATAGTCACAAATTATGCCTA+TGG + tig0030547:8331-8350 None:intergenic 30.0%
TGATTGAACTCTTCATATTC+AGG - tig0030547:8388-8407 Msa1443830:intron 30.0%
!! ATAGTTTTTCGATTGGGTTA+TGG - tig0030547:8738-8757 Msa1443830:intron 30.0%
!! TAGTTTTTCGATTGGGTTAT+GGG - tig0030547:8739-8758 Msa1443830:intron 30.0%
CTCAATGTCAAGAATTTACA+TGG + tig0030547:8853-8872 None:intergenic 30.0%
CATGTAAATTCTTGACATTG+AGG - tig0030547:8851-8870 Msa1443830:intron 30.0%
! TAGCATTGAGATAAAATGAC+AGG - tig0030547:8878-8897 Msa1443830:intron 30.0%
TATTCATTCACTTTCAAACC+CGG - tig0030547:8910-8929 Msa1443830:intron 30.0%
TTAAGGTTATGGAGTTATTC+AGG - tig0030547:9099-9118 Msa1443830:intron 30.0%
TAATAGGTAATAAATGAGGC+TGG - tig0030547:9221-9240 Msa1443830:intron 30.0%
! TTCCATATATTTAGGATTGG+GGG + tig0030547:9286-9305 None:intergenic 30.0%
GCTTAAATCAAAAGGGTAAA+TGG + tig0030547:9339-9358 None:intergenic 30.0%
TGCAAAGGCTTAAATCAAAA+GGG + tig0030547:9346-9365 None:intergenic 30.0%
TTCATATGGTTAAACTGCAA+AGG + tig0030547:9361-9380 None:intergenic 30.0%
!! AACCATTTTCCTTAGCTTTT+GGG + tig0030547:9486-9505 None:intergenic 30.0%
!! AAACCATTTTCCTTAGCTTT+TGG + tig0030547:9487-9506 None:intergenic 30.0%
! TTTTCTTGAACAATCAAACC+CGG - tig0030547:9646-9665 Msa1443830:intron 30.0%
AGTTTACATGAACAGATTCA+CGG + tig0030547:9773-9792 None:intergenic 30.0%
ACAACGCAAAAGATATCTAA+TGG + tig0030547:9950-9969 None:intergenic 30.0%
! ATTAGATATCTTTTGCGTTG+TGG - tig0030547:9949-9968 Msa1443830:intron 30.0%
!! TGTAAAGTTGATATGCAATC+AGG - tig0030547:9978-9997 Msa1443830:intron 30.0%
AAGTGAGAAACATCATAATC+TGG + tig0030547:10123-10142 None:intergenic 30.0%
! TTGCTGTTAAAATGCAAAAC+TGG - tig0030547:10283-10302 Msa1443830:intron 30.0%
AATTTAAACCATGTGACTTG+TGG + tig0030547:10392-10411 None:intergenic 30.0%
TGTAAACCATCATATTCAAG+CGG - tig0030547:10454-10473 Msa1443830:intron 30.0%
!! AAAAGGTACTCTCAAACTAA+AGG - tig0030547:10529-10548 Msa1443830:intron 30.0%
! TAAAGCCGATGGTATTTTAT+AGG - tig0030547:10573-10592 Msa1443830:intron 30.0%
TCTGTCCTATAAAATACCAT+CGG + tig0030547:10581-10600 None:intergenic 30.0%
!!! CACTAGTTTTATTCATACGT+TGG + tig0030547:10621-10640 None:intergenic 30.0%
ATTAGGTATGATTGCATTAG+AGG - tig0030547:10694-10713 Msa1443830:intron 30.0%
TTAGGTATGATTGCATTAGA+GGG - tig0030547:10695-10714 Msa1443830:intron 30.0%
AAGGAAATTGTCAAGGATAA+TGG + tig0030547:11052-11071 None:intergenic 30.0%
CAATTTCCTTAATTTCTTGC+AGG - tig0030547:11062-11081 Msa1443830:intron 30.0%
CCAAAAACGCCTAAAATAAA+GGG + tig0030547:11155-11174 None:intergenic 30.0%
TCCAAAAACGCCTAAAATAA+AGG + tig0030547:11156-11175 None:intergenic 30.0%
CGAGTGTTACAAGTAAAATA+AGG + tig0030547:11489-11508 None:intergenic 30.0%
ACTTGTAACACTCGTAAAAT+TGG - tig0030547:11495-11514 Msa1443830:five_prime_UTR 30.0%
ATCACATCTCTATACTACTT+TGG + tig0030547:11631-11650 None:intergenic 30.0%
GATTTGCAAAAACCGTGAAA+TGG - tig0030547:7379-7398 Msa1443830:three_prime_UTR 35.0%
! CTGTTCAACAAGTTTAGAAC+GGG + tig0030547:7532-7551 None:intergenic 35.0%
GCTGTTCAACAAGTTTAGAA+CGG + tig0030547:7533-7552 None:intergenic 35.0%
CCAAGTAATGATATGAGGTT+TGG + tig0030547:7613-7632 None:intergenic 35.0%
CCAAACCTCATATCATTACT+TGG - tig0030547:7610-7629 Msa1443830:three_prime_UTR 35.0%
TGAATGTATTCACATGTGTG+AGG - tig0030547:7832-7851 Msa1443830:CDS 35.0%
GAATGTATTCACATGTGTGA+GGG - tig0030547:7833-7852 Msa1443830:CDS 35.0%
AAGTAGTCACTTCAGATCAA+AGG + tig0030547:7885-7904 None:intergenic 35.0%
TATGCCCATTTCAATGTTTC+AGG + tig0030547:8032-8051 None:intergenic 35.0%
ATTGAAACCGAGACTTTGTT+TGG - tig0030547:8088-8107 Msa1443830:intron 35.0%
! TTGAAACCGAGACTTTGTTT+GGG - tig0030547:8089-8108 Msa1443830:intron 35.0%
! TCTTAAAGTGTTTCACCTAG+TGG - tig0030547:8275-8294 Msa1443830:intron 35.0%
! CTTAAAGTGTTTCACCTAGT+GGG - tig0030547:8276-8295 Msa1443830:intron 35.0%
! CAAACAAAACTTTTCCCACT+AGG + tig0030547:8293-8312 None:intergenic 35.0%
AGTCACAAATTATGCCTATG+GGG + tig0030547:8329-8348 None:intergenic 35.0%
CACTGCAATTAACTAGTGTT+GGG - tig0030547:8442-8461 Msa1443830:intron 35.0%
GATGTAGCACTATATTGTAG+CGG - tig0030547:8522-8541 Msa1443830:intron 35.0%
AGAATTTACATGGACAAGGA+AGG + tig0030547:8843-8862 None:intergenic 35.0%
GTCAAGAATTTACATGGACA+AGG + tig0030547:8847-8866 None:intergenic 35.0%
ATTCATTCACTTTCAAACCC+GGG - tig0030547:8911-8930 Msa1443830:intron 35.0%
GCAGAAAACTCAACATAGTA+TGG + tig0030547:8947-8966 None:intergenic 35.0%
! TACTATGTTGAGTTTTCTGC+TGG - tig0030547:8947-8966 Msa1443830:intron 35.0%
! TTTTCCTGAGATTTAACAGC+AGG + tig0030547:9144-9163 None:intergenic 35.0%
! ACATCTGTTGTGAGCTTTAT+AGG - tig0030547:9187-9206 Msa1443830:intron 35.0%
!!! GCTGCATTTTTAGGTTAGAA+AGG - tig0030547:9251-9270 Msa1443830:intron 35.0%
CTGCAAAGGCTTAAATCAAA+AGG + tig0030547:9347-9366 None:intergenic 35.0%
TTTCCAGTAGCAAAACCTTT+GGG + tig0030547:9418-9437 None:intergenic 35.0%
!! ACCATTTTCCTTAGCTTTTG+GGG + tig0030547:9485-9504 None:intergenic 35.0%
TCAATGCACTTTGCCTTTAT+TGG - tig0030547:9607-9626 Msa1443830:intron 35.0%
CAATGCACTTTGCCTTTATT+GGG - tig0030547:9608-9627 Msa1443830:intron 35.0%
TTTCTTGAACAATCAAACCC+GGG - tig0030547:9647-9666 Msa1443830:intron 35.0%
AAATTAAGACTTGAGTTCCC+CGG + tig0030547:9668-9687 None:intergenic 35.0%
AGATATAGTGATAGCATCAG+TGG + tig0030547:9808-9827 None:intergenic 35.0%
! CAGTACTGGAGCTATAAAAT+TGG - tig0030547:9886-9905 Msa1443830:intron 35.0%
TGGAGCTATAAAATTGGCTA+AGG - tig0030547:9892-9911 Msa1443830:intron 35.0%
GGAGCTATAAAATTGGCTAA+GGG - tig0030547:9893-9912 Msa1443830:intron 35.0%
!! AAGTTGATATGCAATCAGGT+AGG - tig0030547:9982-10001 Msa1443830:intron 35.0%
! AGTTGATATGCAATCAGGTA+GGG - tig0030547:9983-10002 Msa1443830:intron 35.0%
! TAGGGTGTTCTGTATTCTAT+TGG - tig0030547:10001-10020 Msa1443830:intron 35.0%
! AGGGTGTTCTGTATTCTATT+GGG - tig0030547:10002-10021 Msa1443830:intron 35.0%
TGCAATAAGATCTTGCACAA+GGG + tig0030547:10200-10219 None:intergenic 35.0%
AAAACTGGATTTCTGATTCC+AGG - tig0030547:10298-10317 Msa1443830:intron 35.0%
TCTACCATCATTTCGTACAA+TGG + tig0030547:10423-10442 None:intergenic 35.0%
AAATGATGGTAGAATGAGTG+AGG - tig0030547:10430-10449 Msa1443830:intron 35.0%
GTAAACCATCATATTCAAGC+GGG - tig0030547:10455-10474 Msa1443830:intron 35.0%
TAAACCATCATATTCAAGCG+GGG - tig0030547:10456-10475 Msa1443830:intron 35.0%
GTGGTTGAAAATTGAAATGG+AGG - tig0030547:10478-10497 Msa1443830:intron 35.0%
GTTGAAAATTGAAATGGAGG+AGG - tig0030547:10481-10500 Msa1443830:intron 35.0%
CACTGAATGTGTAGAAATAC+TGG - tig0030547:10661-10680 Msa1443830:intron 35.0%
GAATGTGTAGAAATACTGGA+CGG - tig0030547:10665-10684 Msa1443830:intron 35.0%
AATGTGTAGAAATACTGGAC+GGG - tig0030547:10666-10685 Msa1443830:intron 35.0%
ATCAACCCTGCAAGAAATTA+AGG + tig0030547:11071-11090 None:intergenic 35.0%
!!! CCCTTTATTTTAGGCGTTTT+TGG - tig0030547:11152-11171 Msa1443830:intron 35.0%
AAGAATGCTCATATGGACAA+TGG + tig0030547:11271-11290 None:intergenic 35.0%
TGACAGCACTCAAATATTCA+TGG + tig0030547:11366-11385 None:intergenic 35.0%
!! TTCACTTAGCTTGTGCATAT+GGG + tig0030547:11456-11475 None:intergenic 35.0%
!! ATTCACTTAGCTTGTGCATA+TGG + tig0030547:11457-11476 None:intergenic 35.0%
GAAATTTCATGCTTCTCTGT+AGG + tig0030547:11522-11541 None:intergenic 35.0%
ATTTATGCTGAGATCGGATC+TGG + tig0030547:7566-7585 None:intergenic 40.0%
TTCAATGACTGACTAATCCG+AGG - tig0030547:7940-7959 Msa1443830:intron 40.0%
TCAATGACTGACTAATCCGA+GGG - tig0030547:7941-7960 Msa1443830:intron 40.0%
TCGGACCTGAAACATTGAAA+TGG - tig0030547:8024-8043 Msa1443830:intron 40.0%
CGGACCTGAAACATTGAAAT+GGG - tig0030547:8025-8044 Msa1443830:intron 40.0%
ATTGAAATGGGCATACTCCA+AGG - tig0030547:8037-8056 Msa1443830:intron 40.0%
GTAAGTCCCAAACAAAGTCT+CGG + tig0030547:8098-8117 None:intergenic 40.0%
TAATGAAATGCCACCTGCTA+AGG - tig0030547:8220-8239 Msa1443830:intron 40.0%
TAAGATTCATCCTTAGCAGG+TGG + tig0030547:8233-8252 None:intergenic 40.0%
TGGTAAGATTCATCCTTAGC+AGG + tig0030547:8236-8255 None:intergenic 40.0%
ACATCTCTCATTCTTTCAGC+TGG + tig0030547:8256-8275 None:intergenic 40.0%
!! GTTTAAAAGGGTATCCCCAT+AGG - tig0030547:8312-8331 Msa1443830:intron 40.0%
TGGAAGTAGATCGTGATCTT+AGG + tig0030547:8424-8443 None:intergenic 40.0%
CCAACACTAGTTAATTGCAG+TGG + tig0030547:8444-8463 None:intergenic 40.0%
CCACTGCAATTAACTAGTGT+TGG - tig0030547:8441-8460 Msa1443830:intron 40.0%
! TCCTCAAATTACGGCTTTAG+TGG - tig0030547:8595-8614 Msa1443830:intron 40.0%
ACCACTAAAGCCGTAATTTG+AGG + tig0030547:8599-8618 None:intergenic 40.0%
! GTAGGTAGGTGTTATGCTTA+TGG + tig0030547:8985-9004 None:intergenic 40.0%
ATCCTCGAAATAGTGTAGGT+AGG + tig0030547:8999-9018 None:intergenic 40.0%
CTACATCCTCGAAATAGTGT+AGG + tig0030547:9003-9022 None:intergenic 40.0%
GGATGTAGTCGAAATTATGC+TGG - tig0030547:9015-9034 Msa1443830:intron 40.0%
TTTAGGCCTCTTGTGCTTTA+AGG - tig0030547:9082-9101 Msa1443830:intron 40.0%
CCATAACCTTAAAGCACAAG+AGG + tig0030547:9091-9110 None:intergenic 40.0%
!! CCTCTTGTGCTTTAAGGTTA+TGG - tig0030547:9088-9107 Msa1443830:intron 40.0%
TGCCCCCAATCCTAAATATA+TGG - tig0030547:9281-9300 Msa1443830:intron 40.0%
! AAATGGGAAAGTCTGGAACT+AGG + tig0030547:9322-9341 None:intergenic 40.0%
AAAGGGTAAATGGGAAAGTC+TGG + tig0030547:9329-9348 None:intergenic 40.0%
CTTTCCAGTAGCAAAACCTT+TGG + tig0030547:9419-9438 None:intergenic 40.0%
!! CCATTTTCCTTAGCTTTTGG+GGG + tig0030547:9484-9503 None:intergenic 40.0%
CTTTGCCTTTATTGGGATCA+CGG - tig0030547:9615-9634 Msa1443830:intron 40.0%
TTCTTGAACAATCAAACCCG+GGG - tig0030547:9648-9667 Msa1443830:intron 40.0%
AATTAAGACTTGAGTTCCCC+GGG + tig0030547:9667-9686 None:intergenic 40.0%
TTAAGCTGTGGTCCAATCAA+TGG + tig0030547:9858-9877 None:intergenic 40.0%
! TTTTATAGCTCCAGTACTGC+CGG + tig0030547:9885-9904 None:intergenic 40.0%
TGTTCCATGTTAGTTGACCA+AGG - tig0030547:10151-10170 Msa1443830:intron 40.0%
TGAACCTTGGTCAACTAACA+TGG + tig0030547:10158-10177 None:intergenic 40.0%
GTGCAATAAGATCTTGCACA+AGG + tig0030547:10201-10220 None:intergenic 40.0%
ATTCCAGGCAGGATTTATGT+AGG - tig0030547:10313-10332 Msa1443830:intron 40.0%
CAGGATTTATGTAGGTCATG+TGG - tig0030547:10321-10340 Msa1443830:intron 40.0%
AAGCTTCGATAGCTTAGACA+TGG - tig0030547:10346-10365 Msa1443830:intron 40.0%
GATAGCTTAGACATGGATGT+TGG - tig0030547:10353-10372 Msa1443830:intron 40.0%
! ATGTGACTTGTGGTATGACA+TGG + tig0030547:10382-10401 None:intergenic 40.0%
ATGTCATACCACAAGTCACA+TGG - tig0030547:10381-10400 Msa1443830:intron 40.0%
AGGTCCATTGTACGAAATGA+TGG - tig0030547:10416-10435 Msa1443830:intron 40.0%
GGGGTGGTTGAAAATTGAAA+TGG - tig0030547:10475-10494 Msa1443830:intron 40.0%
TTATAGGACAGATTGTTGGG+TGG - tig0030547:10589-10608 Msa1443830:intron 40.0%
! TTAGATGGTTTGTGCGTATG+TGG - tig0030547:10756-10775 Msa1443830:intron 40.0%
AAGGAAAGTAGATCAGACGA+AGG - tig0030547:10802-10821 Msa1443830:intron 40.0%
AAAGTAGATCAGACGAAGGA+TGG - tig0030547:10806-10825 Msa1443830:intron 40.0%
CTTATTCCACTAGGTCTACA+TGG + tig0030547:10945-10964 None:intergenic 40.0%
CATGTAGACCTAGTGGAATA+AGG - tig0030547:10943-10962 Msa1443830:intron 40.0%
! ATGGGTTCTGTTGTTGTTGT+GGG - tig0030547:10967-10986 Msa1443830:intron 40.0%
ATAAAGGGTCAAGCAGGATA+TGG + tig0030547:11140-11159 None:intergenic 40.0%
CCTAAAATAAAGGGTCAAGC+AGG + tig0030547:11146-11165 None:intergenic 40.0%
!!! CCTGCTTGACCCTTTATTTT+AGG - tig0030547:11143-11162 Msa1443830:intron 40.0%
GTCCATATGAGCATTCTTTG+TGG - tig0030547:11273-11292 Msa1443830:intron 40.0%
CACCACAAAGAATGCTCATA+TGG + tig0030547:11278-11297 None:intergenic 40.0%
TCTTTGTGGTGATATCTCAC+AGG - tig0030547:11287-11306 Msa1443830:intron 40.0%
ATATCTCACAGGATGTACAC+TGG - tig0030547:11298-11317 Msa1443830:intron 40.0%
AGTGCTGTCATTACTAGTGT+AGG - tig0030547:11376-11395 Msa1443830:intron 40.0%
CCATCAACACTCAAAAACGT+AGG + tig0030547:11418-11437 None:intergenic 40.0%
!!! CCTACGTTTTTGAGTGTTGA+TGG - tig0030547:11415-11434 Msa1443830:CDS 40.0%
! TGAGGAATTTCCTTTTTCCC+AGG - tig0030547:11568-11587 Msa1443830:five_prime_UTR 40.0%
!! TGCTGTGACATTTCTAGCAT+AGG - tig0030547:11597-11616 Msa1443830:five_prime_UTR 40.0%
! GATCTGAGACAAACACGGTT+TGG - tig0030547:7439-7458 Msa1443830:three_prime_UTR 45.0%
TTGGGAGAGAAGAAAGTGAG+AGG + tig0030547:7594-7613 None:intergenic 45.0%
! ATTCACATGTGTGAGGGTGT+TGG - tig0030547:7839-7858 Msa1443830:CDS 45.0%
! CAAAGGCGTGTCAAACTATC+TGG + tig0030547:7868-7887 None:intergenic 45.0%
CAATGACTGACTAATCCGAG+GGG - tig0030547:7942-7961 Msa1443830:intron 45.0%
AATGACTGACTAATCCGAGG+GGG - tig0030547:7943-7962 Msa1443830:intron 45.0%
TAGATTTAAATGGGCCCCCT+CGG + tig0030547:7960-7979 None:intergenic 45.0%
CTAGAGAGCAAAGCTCTGTA+AGG - tig0030547:7977-7996 Msa1443830:intron 45.0%
!! CTCTGTAAGGACTAGCCTAT+TGG - tig0030547:7990-8009 Msa1443830:intron 45.0%
GTTTCAATTCAACTCCGTCG+TGG + tig0030547:8076-8095 None:intergenic 45.0%
TGCAAGACTAACCTTGAGAC+TGG - tig0030547:8350-8369 Msa1443830:intron 45.0%
GCAAGACTAACCTTGAGACT+GGG - tig0030547:8351-8370 Msa1443830:intron 45.0%
AGTCCACCATCAAATTCTGG+GGG + tig0030547:8820-8839 None:intergenic 45.0%
GAGTCCACCATCAAATTCTG+GGG + tig0030547:8821-8840 None:intergenic 45.0%
GGAGTCCACCATCAAATTCT+GGG + tig0030547:8822-8841 None:intergenic 45.0%
AGGAGTCCACCATCAAATTC+TGG + tig0030547:8823-8842 None:intergenic 45.0%
TAGTATGGCATTCAGATGCC+CGG + tig0030547:8932-8951 None:intergenic 45.0%
CACCTACCTACACTATTTCG+AGG - tig0030547:8994-9013 Msa1443830:intron 45.0%
GACACCTGCTGTTAAATCTC+AGG - tig0030547:9137-9156 Msa1443830:intron 45.0%
! GGTGTGACAGCTGCATTTTT+AGG - tig0030547:9242-9261 Msa1443830:intron 45.0%
! TAGCCCAAAGGTTTTGCTAC+TGG - tig0030547:9412-9431 Msa1443830:intron 45.0%
ATCGAAACCCCCAAAAGCTA+AGG - tig0030547:9474-9493 Msa1443830:intron 45.0%
CCCCCAAAAGCTAAGGAAAA+TGG - tig0030547:9481-9500 Msa1443830:intron 45.0%
GAGATCCGTGATCCCAATAA+AGG + tig0030547:9623-9642 None:intergenic 45.0%
TTTATAGCTCCAGTACTGCC+GGG + tig0030547:9884-9903 None:intergenic 45.0%
TTGACCAAGGTTCATAACCC+TGG - tig0030547:10164-10183 Msa1443830:intron 45.0%
TGACCTACATAAATCCTGCC+TGG + tig0030547:10319-10338 None:intergenic 45.0%
ATTGAAATGGAGGAGGCTCT+TGG - tig0030547:10488-10507 Msa1443830:intron 45.0%
TTGAAATGGAGGAGGCTCTT+GGG - tig0030547:10489-10508 Msa1443830:intron 45.0%
!! CCATCGGCTTTAGTGTTGTA+AGG + tig0030547:10565-10584 None:intergenic 45.0%
CCTTACAACACTAAAGCCGA+TGG - tig0030547:10562-10581 Msa1443830:intron 45.0%
ATACTGGACGGGATGAGATT+AGG - tig0030547:10677-10696 Msa1443830:intron 45.0%
! GCTTTATCGCTAGAAGCACA+AGG - tig0030547:10828-10847 Msa1443830:intron 45.0%
GTTGATCCATGTAGACCTAG+TGG - tig0030547:10936-10955 Msa1443830:intron 45.0%
AGACCTAGTGGAATAAGGCA+TGG - tig0030547:10948-10967 Msa1443830:intron 45.0%
GACCTAGTGGAATAAGGCAT+GGG - tig0030547:10949-10968 Msa1443830:intron 45.0%
! AACCCATGCCTTATTCCACT+AGG + tig0030547:10954-10973 None:intergenic 45.0%
! CATGGGTTCTGTTGTTGTTG+TGG - tig0030547:10966-10985 Msa1443830:intron 45.0%
TCATACGCCTAAGAGCTTCT+AGG - tig0030547:11212-11231 Msa1443830:intron 45.0%
AGGTTTGCCTAGAAGCTCTT+AGG + tig0030547:11222-11241 None:intergenic 45.0%
AAGAGCTTCTAGGCAAACCT+TGG - tig0030547:11222-11241 Msa1443830:intron 45.0%
AGCAGAAGATCCTGGGAAAA+AGG + tig0030547:11581-11600 None:intergenic 45.0%
ATGTCACAGCAGAAGATCCT+GGG + tig0030547:11588-11607 None:intergenic 45.0%
AATGTCACAGCAGAAGATCC+TGG + tig0030547:11589-11608 None:intergenic 45.0%
!!! CATTTTTTTAAAAAATATTT+GGG - tig0030547:9532-9551 Msa1443830:intron 5.0%
!!! ATTTTTTTAAAAAATATTTG+GGG - tig0030547:9533-9552 Msa1443830:intron 5.0%
!! ATGAAGCACTGACACAGACG+CGG - tig0030547:7770-7789 Msa1443830:CDS 50.0%
!! AAGGACTAGCCTATTGGCAC+TGG - tig0030547:7996-8015 Msa1443830:intron 50.0%
! CCTATTGGCACTGGAGGAAT+CGG - tig0030547:8005-8024 Msa1443830:intron 50.0%
ATTCAACTCCGTCGTGGTGT+TGG + tig0030547:8070-8089 None:intergenic 50.0%
CTAACCTTGAGACTGGGTAG+TGG - tig0030547:8357-8376 Msa1443830:intron 50.0%
CTAACCACTACCCAGTCTCA+AGG + tig0030547:8364-8383 None:intergenic 50.0%
! CTTGAGACTGGGTAGTGGTT+AGG - tig0030547:8362-8381 Msa1443830:intron 50.0%
!! TTAGTGGTGCCATAGCACTG+CGG - tig0030547:8611-8630 Msa1443830:intron 50.0%
!! GGGGGTATCGAAAACTAGCT+GGG + tig0030547:8801-8820 None:intergenic 50.0%
CGATACCCCCCAGAATTTGA+TGG - tig0030547:8811-8830 Msa1443830:intron 50.0%
TACCCCCCAGAATTTGATGG+TGG - tig0030547:8814-8833 Msa1443830:intron 50.0%
GTCCACCATCAAATTCTGGG+GGG + tig0030547:8819-8838 None:intergenic 50.0%
AGTATGGCATTCAGATGCCC+GGG + tig0030547:8931-8950 None:intergenic 50.0%
! GGGAAAGTCTGGAACTAGGA+CGG + tig0030547:9318-9337 None:intergenic 50.0%
CTGGATTTCTGATTCCAGGC+AGG - tig0030547:10302-10321 Msa1443830:intron 50.0%
ACCATCATATTCAAGCGGGG+TGG - tig0030547:10459-10478 Msa1443830:intron 50.0%
ACCACCCCGCTTGAATATGA+TGG + tig0030547:10463-10482 None:intergenic 50.0%
! GATGGTTTGTGCGTATGTGG+AGG - tig0030547:10759-10778 Msa1443830:intron 50.0%
!! TTGCAGGGTTGATGTTGCTG+TGG - tig0030547:11078-11097 Msa1443830:intron 50.0%
CTTCTAGGCAAACCTTGGCA+AGG - tig0030547:11227-11246 Msa1443830:intron 50.0%
GCAAACCTTGGCAAGGAAGA+GGG - tig0030547:11234-11253 Msa1443830:intron 50.0%
TCTTTCCCTCTTCCTTGCCA+AGG + tig0030547:11242-11261 None:intergenic 50.0%
GGATGTACACTGGCAGACAA+AGG - tig0030547:11308-11327 Msa1443830:intron 50.0%
!! GACTAGCCTATTGGCACTGG+AGG - tig0030547:7999-8018 Msa1443830:intron 55.0%
CCGATTCCTCCAGTGCCAAT+AGG + tig0030547:8008-8027 None:intergenic 55.0%
GTGCTACATCGCTGCTGTTG+TGG + tig0030547:8512-8531 None:intergenic 55.0%
TCTGCTACACCGCAGTGCTA+TGG + tig0030547:8623-8642 None:intergenic 55.0%
!! GGGGGGTATCGAAAACTAGC+TGG + tig0030547:8802-8821 None:intergenic 55.0%
TGGACCACAGCTTAAGAGCC+CGG - tig0030547:9863-9882 Msa1443830:intron 55.0%
CCTGCCAGGGTTATGAACCT+TGG + tig0030547:10171-10190 None:intergenic 55.0%
CCAAGGTTCATAACCCTGGC+AGG - tig0030547:10168-10187 Msa1443830:intron 55.0%
GGCAAACCTTGGCAAGGAAG+AGG - tig0030547:11233-11252 Msa1443830:intron 55.0%
!! GTGGTGTTGGCTTGGGACCT+TGG + tig0030547:8057-8076 None:intergenic 60.0%
GTCCCAAGCCAACACCACGA+CGG - tig0030547:8059-8078 Msa1443830:intron 60.0%
!! CTCCGTCGTGGTGTTGGCTT+GGG + tig0030547:8064-8083 None:intergenic 60.0%
!! ACTCCGTCGTGGTGTTGGCT+TGG + tig0030547:8065-8084 None:intergenic 60.0%
ACTGCCGGGCTCTTAAGCTG+TGG + tig0030547:9870-9889 None:intergenic 60.0%
GCTTAAGAGCCCGGCAGTAC+TGG - tig0030547:9872-9891 Msa1443830:intron 60.0%
GGTTCATAACCCTGGCAGGC+TGG - tig0030547:10172-10191 Msa1443830:intron 60.0%
ACAAGGGAGCCAGCCTGCCA+GGG + tig0030547:10184-10203 None:intergenic 65.0%
CACAAGGGAGCCAGCCTGCC+AGG + tig0030547:10185-10204 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0030547 gene 7293 11716 7293 ID=Msa1443830;Name=Msa1443830
tig0030547 mRNA 7293 11716 7293 ID=Msa1443830-mRNA-1;Parent=Msa1443830;Name=Msa1443830-mRNA-1;_AED=0.00;_eAED=0.00;_QI=229|1|1|1|0|0.5|2|434|102
tig0030547 exon 7293 7925 7293 ID=Msa1443830-mRNA-1:exon:14137;Parent=Msa1443830-mRNA-1
tig0030547 exon 11378 11716 11378 ID=Msa1443830-mRNA-1:exon:14136;Parent=Msa1443830-mRNA-1
tig0030547 five_prime_UTR 11488 11716 11488 ID=Msa1443830-mRNA-1:five_prime_utr;Parent=Msa1443830-mRNA-1
tig0030547 CDS 11378 11487 11378 ID=Msa1443830-mRNA-1:cds;Parent=Msa1443830-mRNA-1
tig0030547 CDS 7727 7925 7727 ID=Msa1443830-mRNA-1:cds;Parent=Msa1443830-mRNA-1
tig0030547 three_prime_UTR 7293 7726 7293 ID=Msa1443830-mRNA-1:three_prime_utr;Parent=Msa1443830-mRNA-1
Gene Sequence

>Msa1443830

ATGATAACCCGTTCTAAACTTGTTGAACAGCTCAGAGATTACCAGATCCGATCTCAGCATAAATATTCTCCTCTCACTTTCTTCTCTCCCAAACCTCATATCATTACTTGGGTTGATGTTGCTGTGGCAATTGCTTTTGCTCTAGTGTTCTGCATGCTTGTCGCCATATCCTGCTTGACCCTTTATTTTAGGCGTTTTTGGATTTTTCTTCTTGTTGTAAGCTTCAGTATAATTCTTCTCATACGCCTAAGAGCTTCTAGGCAAACCTTGGCAAGGAAGAGGGAAAGAAGATTGCCATTGTCCATATGA
Protein sequence

>Msa1443830

MITRSKLVEQLRDYQIRSQHKYSPLTFFSPKPHIITWVDVAVAIAFALVFCMLVAISCLTLYFRRFWIFLLVVSFSIILLIRLRASRQTLARKRERRLPLSI*