AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0943970


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0943970 MtrunA17_Chr6g0460751 84.211 95 8 1 25 119 1 88 1.69e-53 162
Msa0943970 MtrunA17_Chr7g0254151 49.351 77 34 2 41 116 20 92 1.34e-14 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACCACTCCCAGGGGATTT+TGG 0.070443 6_3:-20302763 None:intergenic
TGAATTTGTAATATCAAATA+AGG 0.210050 6_3:+20302925 Msa0943970:three_prime_UTR
GATGGGAAGAAGGGTCAATT+TGG 0.296094 6_3:+20302696 Msa0943970:CDS
CCACTAACATATTAATATTC+TGG 0.317915 6_3:-20301640 None:intergenic
GCTACATCCTAAACTTTAGA+AGG 0.318376 6_3:+20302811 Msa0943970:three_prime_UTR
CACCAGGCCCTTCATAATCA+TGG 0.396641 6_3:-20302723 None:intergenic
ATATTCTGGCGTACTATACT+AGG 0.411234 6_3:-20301626 None:intergenic
CCAGAATATTAATATGTTAG+TGG 0.424217 6_3:+20301640 Msa0943970:exon
TGAGAAAATGATGGTACATA+TGG 0.459434 6_3:+20302663 Msa0943970:CDS
TTGGAACTCCATGATTATGA+AGG 0.463143 6_3:+20302715 Msa0943970:CDS
ATGAAAGATTGATGGGAAGA+AGG 0.472148 6_3:+20302686 Msa0943970:CDS
TGAAAGATTGATGGGAAGAA+GGG 0.490161 6_3:+20302687 Msa0943970:CDS
ACATATGGATGAAAGATTGA+TGG 0.490804 6_3:+20302678 Msa0943970:CDS
TTATGTTCTTTATTTGCACC+AGG 0.490997 6_3:-20302739 None:intergenic
GCACTAACCTTATATAAAGA+AGG 0.507798 6_3:-20301931 None:intergenic
CTCCATGATTATGAAGGGCC+TGG 0.512793 6_3:+20302721 Msa0943970:CDS
GGAACATGGTTCATTGATGT+TGG 0.518756 6_3:+20302633 Msa0943970:intron
CATATGGATGAAAGATTGAT+GGG 0.518975 6_3:+20302679 Msa0943970:CDS
GAACATAACCCAAAATCCCC+TGG 0.526511 6_3:+20302754 Msa0943970:CDS
AACCCAAAATCCCCTGGGAG+TGG 0.548305 6_3:+20302760 Msa0943970:CDS
TTTGCACATCAACCACTCCC+AGG 0.556615 6_3:-20302772 None:intergenic
TGGAACTCCATGATTATGAA+GGG 0.574524 6_3:+20302716 Msa0943970:CDS
TTGCACATCAACCACTCCCA+GGG 0.587694 6_3:-20302771 None:intergenic
AACATAACCCAAAATCCCCT+GGG 0.609618 6_3:+20302755 Msa0943970:CDS
TTTCATCTTGAATTGGATGG+TGG 0.617406 6_3:-20301708 None:intergenic
AATTAGAATCAAAGTATGTG+TGG 0.621826 6_3:+20302866 Msa0943970:three_prime_UTR
TTGCAGGGATAGCAAAGACA+TGG 0.624053 6_3:-20301781 None:intergenic
TGCACATCAACCACTCCCAG+GGG 0.679834 6_3:-20302770 None:intergenic
TGGATGGTGGAAGATAACAA+AGG 0.717266 6_3:-20301695 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATAAAAATATTAGTGTTT+TGG + chr6_3:20302968-20302987 Msa0943970:three_prime_UTR 10.0%
!!! TAATAAAAATATTAGTGTTT+TGG + chr6_3:20302968-20302987 Msa0943970:three_prime_UTR 10.0%
!!! ATGTATTGAATTGATTTTTT+TGG + chr6_3:20301864-20301883 Msa0943970:intron 15.0%
!!! TTTACTTTTCAAATTTTTGT+TGG + chr6_3:20302207-20302226 Msa0943970:intron 15.0%
!!! TTTTTTATTCTTTTAAATCG+TGG - chr6_3:20302341-20302360 None:intergenic 15.0%
!! TGAATTTGTAATATCAAATA+AGG + chr6_3:20302925-20302944 Msa0943970:three_prime_UTR 15.0%
!!! ATGTATTGAATTGATTTTTT+TGG + chr6_3:20301864-20301883 Msa0943970:intron 15.0%
!!! TTTACTTTTCAAATTTTTGT+TGG + chr6_3:20302207-20302226 Msa0943970:intron 15.0%
!!! TTTTTTATTCTTTTAAATCG+TGG - chr6_3:20302341-20302360 None:intergenic 15.0%
!! TGAATTTGTAATATCAAATA+AGG + chr6_3:20302925-20302944 Msa0943970:three_prime_UTR 15.0%
!!! AGTTTTTTTTCATCTTGAAT+TGG - chr6_3:20301718-20301737 None:intergenic 20.0%
!!! AGTTTTTCCTTCTTTATATA+AGG + chr6_3:20301924-20301943 Msa0943970:CDS 20.0%
!!! CTTCTTAATTTTCATCTATT+TGG + chr6_3:20301953-20301972 Msa0943970:intron 20.0%
!!! ATTTTTTCATCCAATATCTA+AGG + chr6_3:20302018-20302037 Msa0943970:intron 20.0%
!!! AACTTTTTTGGCAAAAATTA+AGG - chr6_3:20302556-20302575 None:intergenic 20.0%
!!! GTTAATTAGTCAAACTTTTT+TGG - chr6_3:20302568-20302587 None:intergenic 20.0%
!!! AAAAACTAATTAGTTTGAAC+TGG + chr6_3:20303002-20303021 Msa0943970:three_prime_UTR 20.0%
!!! AGTTTTTTTTCATCTTGAAT+TGG - chr6_3:20301718-20301737 None:intergenic 20.0%
!!! AGTTTTTCCTTCTTTATATA+AGG + chr6_3:20301924-20301943 Msa0943970:CDS 20.0%
!!! CTTCTTAATTTTCATCTATT+TGG + chr6_3:20301953-20301972 Msa0943970:intron 20.0%
!!! ATTTTTTCATCCAATATCTA+AGG + chr6_3:20302018-20302037 Msa0943970:intron 20.0%
!!! AACTTTTTTGGCAAAAATTA+AGG - chr6_3:20302556-20302575 None:intergenic 20.0%
!!! GTTAATTAGTCAAACTTTTT+TGG - chr6_3:20302568-20302587 None:intergenic 20.0%
!!! AAAAACTAATTAGTTTGAAC+TGG + chr6_3:20303002-20303021 Msa0943970:three_prime_UTR 20.0%
! CCACTAACATATTAATATTC+TGG - chr6_3:20301643-20301662 None:intergenic 25.0%
! CCAGAATATTAATATGTTAG+TGG + chr6_3:20301640-20301659 Msa0943970:exon 25.0%
!!! TTTTTTCATCTTGAATTGGA+TGG - chr6_3:20301714-20301733 None:intergenic 25.0%
!!! GAATTGATTTTTTTGGTTGT+AGG + chr6_3:20301871-20301890 Msa0943970:intron 25.0%
!! ATCTTCACCATTCAAATTTT+TGG - chr6_3:20301899-20301918 None:intergenic 25.0%
!! AAAGTCATGGTTATGTATTT+TGG + chr6_3:20301993-20302012 Msa0943970:intron 25.0%
!! TCATTATTTGTGTGTTTTGT+AGG + chr6_3:20302612-20302631 Msa0943970:intron 25.0%
! AATTAGAATCAAAGTATGTG+TGG + chr6_3:20302866-20302885 Msa0943970:three_prime_UTR 25.0%
! CCACTAACATATTAATATTC+TGG - chr6_3:20301643-20301662 None:intergenic 25.0%
! CCAGAATATTAATATGTTAG+TGG + chr6_3:20301640-20301659 Msa0943970:exon 25.0%
!!! TTTTTTCATCTTGAATTGGA+TGG - chr6_3:20301714-20301733 None:intergenic 25.0%
!!! GAATTGATTTTTTTGGTTGT+AGG + chr6_3:20301871-20301890 Msa0943970:intron 25.0%
!! ATCTTCACCATTCAAATTTT+TGG - chr6_3:20301899-20301918 None:intergenic 25.0%
!! AAAGTCATGGTTATGTATTT+TGG + chr6_3:20301993-20302012 Msa0943970:intron 25.0%
!! TCATTATTTGTGTGTTTTGT+AGG + chr6_3:20302612-20302631 Msa0943970:intron 25.0%
! AATTAGAATCAAAGTATGTG+TGG + chr6_3:20302866-20302885 Msa0943970:three_prime_UTR 25.0%
GTAGGAACCAAAAATTTGAA+TGG + chr6_3:20301889-20301908 Msa0943970:intron 30.0%
GCACTAACCTTATATAAAGA+AGG - chr6_3:20301934-20301953 None:intergenic 30.0%
!! CCTCATTTTTCCTTAGATAT+TGG - chr6_3:20302031-20302050 None:intergenic 30.0%
CCAATATCTAAGGAAAAATG+AGG + chr6_3:20302028-20302047 Msa0943970:intron 30.0%
GGAAAAAAGTGAGAAAATGA+TGG + chr6_3:20302654-20302673 Msa0943970:CDS 30.0%
TGAGAAAATGATGGTACATA+TGG + chr6_3:20302663-20302682 Msa0943970:CDS 30.0%
ACATATGGATGAAAGATTGA+TGG + chr6_3:20302678-20302697 Msa0943970:CDS 30.0%
CATATGGATGAAAGATTGAT+GGG + chr6_3:20302679-20302698 Msa0943970:CDS 30.0%
! TTATGTTCTTTATTTGCACC+AGG - chr6_3:20302742-20302761 None:intergenic 30.0%
!!! GATTTTGCCTTCTAAAGTTT+AGG - chr6_3:20302821-20302840 None:intergenic 30.0%
!!! TGTGTGGTTTTTTGTTTCTT+TGG + chr6_3:20302882-20302901 Msa0943970:three_prime_UTR 30.0%
GTAGGAACCAAAAATTTGAA+TGG + chr6_3:20301889-20301908 Msa0943970:intron 30.0%
GCACTAACCTTATATAAAGA+AGG - chr6_3:20301934-20301953 None:intergenic 30.0%
!! CCTCATTTTTCCTTAGATAT+TGG - chr6_3:20302031-20302050 None:intergenic 30.0%
CCAATATCTAAGGAAAAATG+AGG + chr6_3:20302028-20302047 Msa0943970:intron 30.0%
GGAAAAAAGTGAGAAAATGA+TGG + chr6_3:20302654-20302673 Msa0943970:CDS 30.0%
TGAGAAAATGATGGTACATA+TGG + chr6_3:20302663-20302682 Msa0943970:CDS 30.0%
ACATATGGATGAAAGATTGA+TGG + chr6_3:20302678-20302697 Msa0943970:CDS 30.0%
CATATGGATGAAAGATTGAT+GGG + chr6_3:20302679-20302698 Msa0943970:CDS 30.0%
! TTATGTTCTTTATTTGCACC+AGG - chr6_3:20302742-20302761 None:intergenic 30.0%
!!! GATTTTGCCTTCTAAAGTTT+AGG - chr6_3:20302821-20302840 None:intergenic 30.0%
!!! TGTGTGGTTTTTTGTTTCTT+TGG + chr6_3:20302882-20302901 Msa0943970:three_prime_UTR 30.0%
ATATTCTGGCGTACTATACT+AGG - chr6_3:20301629-20301648 None:intergenic 35.0%
TTTCATCTTGAATTGGATGG+TGG - chr6_3:20301711-20301730 None:intergenic 35.0%
! CAAAAACCTTACTTGTTGCA+GGG - chr6_3:20301799-20301818 None:intergenic 35.0%
! TCAAAAACCTTACTTGTTGC+AGG - chr6_3:20301800-20301819 None:intergenic 35.0%
TTCCTATTCTTGCAAAGTCA+TGG + chr6_3:20301980-20301999 Msa0943970:intron 35.0%
AACCATGACTTTGCAAGAAT+AGG - chr6_3:20301985-20302004 None:intergenic 35.0%
! TTGTGTGTTTTGTAGGAACA+TGG + chr6_3:20302619-20302638 Msa0943970:intron 35.0%
ATGAAAGATTGATGGGAAGA+AGG + chr6_3:20302686-20302705 Msa0943970:CDS 35.0%
TGAAAGATTGATGGGAAGAA+GGG + chr6_3:20302687-20302706 Msa0943970:CDS 35.0%
TTGGAACTCCATGATTATGA+AGG + chr6_3:20302715-20302734 Msa0943970:CDS 35.0%
TGGAACTCCATGATTATGAA+GGG + chr6_3:20302716-20302735 Msa0943970:CDS 35.0%
GCTACATCCTAAACTTTAGA+AGG + chr6_3:20302811-20302830 Msa0943970:three_prime_UTR 35.0%
ATATTCTGGCGTACTATACT+AGG - chr6_3:20301629-20301648 None:intergenic 35.0%
TTTCATCTTGAATTGGATGG+TGG - chr6_3:20301711-20301730 None:intergenic 35.0%
! CAAAAACCTTACTTGTTGCA+GGG - chr6_3:20301799-20301818 None:intergenic 35.0%
! TCAAAAACCTTACTTGTTGC+AGG - chr6_3:20301800-20301819 None:intergenic 35.0%
TTCCTATTCTTGCAAAGTCA+TGG + chr6_3:20301980-20301999 Msa0943970:intron 35.0%
AACCATGACTTTGCAAGAAT+AGG - chr6_3:20301985-20302004 None:intergenic 35.0%
! TTGTGTGTTTTGTAGGAACA+TGG + chr6_3:20302619-20302638 Msa0943970:intron 35.0%
ATGAAAGATTGATGGGAAGA+AGG + chr6_3:20302686-20302705 Msa0943970:CDS 35.0%
TGAAAGATTGATGGGAAGAA+GGG + chr6_3:20302687-20302706 Msa0943970:CDS 35.0%
TTGGAACTCCATGATTATGA+AGG + chr6_3:20302715-20302734 Msa0943970:CDS 35.0%
TGGAACTCCATGATTATGAA+GGG + chr6_3:20302716-20302735 Msa0943970:CDS 35.0%
GCTACATCCTAAACTTTAGA+AGG + chr6_3:20302811-20302830 Msa0943970:three_prime_UTR 35.0%
!! TGGATGGTGGAAGATAACAA+AGG - chr6_3:20301698-20301717 None:intergenic 40.0%
CAACAATTGTGTCAAGCAGA+AGG + chr6_3:20302161-20302180 Msa0943970:intron 40.0%
GGAACATGGTTCATTGATGT+TGG + chr6_3:20302633-20302652 Msa0943970:intron 40.0%
AACATAACCCAAAATCCCCT+GGG + chr6_3:20302755-20302774 Msa0943970:CDS 40.0%
!! TGGATGGTGGAAGATAACAA+AGG - chr6_3:20301698-20301717 None:intergenic 40.0%
CAACAATTGTGTCAAGCAGA+AGG + chr6_3:20302161-20302180 Msa0943970:intron 40.0%
GGAACATGGTTCATTGATGT+TGG + chr6_3:20302633-20302652 Msa0943970:intron 40.0%
AACATAACCCAAAATCCCCT+GGG + chr6_3:20302755-20302774 Msa0943970:CDS 40.0%
TTGCAGGGATAGCAAAGACA+TGG - chr6_3:20301784-20301803 None:intergenic 45.0%
TGCTATCCCTGCAACAAGTA+AGG + chr6_3:20301790-20301809 Msa0943970:intron 45.0%
GATGGGAAGAAGGGTCAATT+TGG + chr6_3:20302696-20302715 Msa0943970:CDS 45.0%
GAACATAACCCAAAATCCCC+TGG + chr6_3:20302754-20302773 Msa0943970:CDS 45.0%
TTGCAGGGATAGCAAAGACA+TGG - chr6_3:20301784-20301803 None:intergenic 45.0%
TGCTATCCCTGCAACAAGTA+AGG + chr6_3:20301790-20301809 Msa0943970:intron 45.0%
GATGGGAAGAAGGGTCAATT+TGG + chr6_3:20302696-20302715 Msa0943970:CDS 45.0%
GAACATAACCCAAAATCCCC+TGG + chr6_3:20302754-20302773 Msa0943970:CDS 45.0%
!!! ATTAACTTTTTAATTTATTT+TGG + chr6_3:20302582-20302601 Msa0943970:intron 5.0%
!!! ATTAACTTTTTAATTTATTT+TGG + chr6_3:20302582-20302601 Msa0943970:intron 5.0%
CTCCATGATTATGAAGGGCC+TGG + chr6_3:20302721-20302740 Msa0943970:CDS 50.0%
CACCAGGCCCTTCATAATCA+TGG - chr6_3:20302726-20302745 None:intergenic 50.0%
! AACCACTCCCAGGGGATTTT+GGG - chr6_3:20302765-20302784 None:intergenic 50.0%
TTGCACATCAACCACTCCCA+GGG - chr6_3:20302774-20302793 None:intergenic 50.0%
TTTGCACATCAACCACTCCC+AGG - chr6_3:20302775-20302794 None:intergenic 50.0%
CTCCATGATTATGAAGGGCC+TGG + chr6_3:20302721-20302740 Msa0943970:CDS 50.0%
CACCAGGCCCTTCATAATCA+TGG - chr6_3:20302726-20302745 None:intergenic 50.0%
! AACCACTCCCAGGGGATTTT+GGG - chr6_3:20302765-20302784 None:intergenic 50.0%
TTGCACATCAACCACTCCCA+GGG - chr6_3:20302774-20302793 None:intergenic 50.0%
TTTGCACATCAACCACTCCC+AGG - chr6_3:20302775-20302794 None:intergenic 50.0%
AACCCAAAATCCCCTGGGAG+TGG + chr6_3:20302760-20302779 Msa0943970:CDS 55.0%
! CAACCACTCCCAGGGGATTT+TGG - chr6_3:20302766-20302785 None:intergenic 55.0%
TGCACATCAACCACTCCCAG+GGG - chr6_3:20302773-20302792 None:intergenic 55.0%
AACCCAAAATCCCCTGGGAG+TGG + chr6_3:20302760-20302779 Msa0943970:CDS 55.0%
! CAACCACTCCCAGGGGATTT+TGG - chr6_3:20302766-20302785 None:intergenic 55.0%
TGCACATCAACCACTCCCAG+GGG - chr6_3:20302773-20302792 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 20301597 20303131 20301597 ID=Msa0943970;Name=Msa0943970
chr6_3 mRNA 20301597 20303131 20301597 ID=Msa0943970-mRNA-1;Parent=Msa0943970;Name=Msa0943970-mRNA-1;_AED=0.00;_eAED=0.00;_QI=56|1|1|1|1|1|3|345|119
chr6_3 exon 20301597 20301806 20301597 ID=Msa0943970-mRNA-1:exon:6030;Parent=Msa0943970-mRNA-1
chr6_3 exon 20301893 20301945 20301893 ID=Msa0943970-mRNA-1:exon:6031;Parent=Msa0943970-mRNA-1
chr6_3 exon 20302634 20303131 20302634 ID=Msa0943970-mRNA-1:exon:6032;Parent=Msa0943970-mRNA-1
chr6_3 five_prime_UTR 20301597 20301652 20301597 ID=Msa0943970-mRNA-1:five_prime_utr;Parent=Msa0943970-mRNA-1
chr6_3 CDS 20301653 20301806 20301653 ID=Msa0943970-mRNA-1:cds;Parent=Msa0943970-mRNA-1
chr6_3 CDS 20301893 20301945 20301893 ID=Msa0943970-mRNA-1:cds;Parent=Msa0943970-mRNA-1
chr6_3 CDS 20302634 20302786 20302634 ID=Msa0943970-mRNA-1:cds;Parent=Msa0943970-mRNA-1
chr6_3 three_prime_UTR 20302787 20303131 20302787 ID=Msa0943970-mRNA-1:three_prime_utr;Parent=Msa0943970-mRNA-1
Gene Sequence

>Msa0943970

ATGTTAGTGGCACTATATAACAAGCATAAAGTATTCATACATCCTTTGTTATCTTCCACCATCCAATTCAAGATGAAAAAAAACTTTTTCATACTTTTTGTGATACTTCTGAGTTTCTCATATATTCTCCATGTCTTTGCTATCCCTGCAACAAGAACCAAAAATTTGAATGGTGAAGATGAATCAGTTTTTCCTTCTTTATATAAGGAACATGGTTCATTGATGTTGGAAAAAAGTGAGAAAATGATGGTACATATGGATGAAAGATTGATGGGAAGAAGGGTCAATTTGGAACTCCATGATTATGAAGGGCCTGGTGCAAATAAAGAACATAACCCAAAATCCCCTGGGAGTGGTTGA

Protein sequence

>Msa0943970

MLVALYNKHKVFIHPLLSSTIQFKMKKNFFILFVILLSFSYILHVFAIPATRTKNLNGEDESVFPSLYKEHGSLMLEKSEKMMVHMDERLMGRRVNLELHDYEGPGANKEHNPKSPGSG*