AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0944020


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0944020 MtrunA17_Chr6g0460751 84.211 95 8 1 25 119 1 88 1.69e-53 162
Msa0944020 MtrunA17_Chr7g0254151 49.351 77 34 2 41 116 20 92 1.34e-14 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACCACTCCCAGGGGATTT+TGG 0.070443 6_3:+20393191 None:intergenic
TGAATTTGTAATATCAAATA+AGG 0.210050 6_3:-20393029 Msa0944020:three_prime_UTR
GATGGGAAGAAGGGTCAATT+TGG 0.296094 6_3:-20393258 Msa0944020:CDS
CCACTAACATATTAATATTC+TGG 0.317915 6_3:+20394314 None:intergenic
GCTACATCCTAAACTTTAGA+AGG 0.318376 6_3:-20393143 Msa0944020:three_prime_UTR
CACCAGGCCCTTCATAATCA+TGG 0.396641 6_3:+20393231 None:intergenic
ATATTCTGGCGTACTATACT+AGG 0.411234 6_3:+20394328 None:intergenic
CCAGAATATTAATATGTTAG+TGG 0.424217 6_3:-20394314 Msa0944020:exon
TGAGAAAATGATGGTACATA+TGG 0.459434 6_3:-20393291 Msa0944020:CDS
TTGGAACTCCATGATTATGA+AGG 0.463143 6_3:-20393239 Msa0944020:CDS
ATGAAAGATTGATGGGAAGA+AGG 0.472148 6_3:-20393268 Msa0944020:CDS
TGAAAGATTGATGGGAAGAA+GGG 0.490161 6_3:-20393267 Msa0944020:CDS
ACATATGGATGAAAGATTGA+TGG 0.490804 6_3:-20393276 Msa0944020:CDS
TTATGTTCTTTATTTGCACC+AGG 0.490997 6_3:+20393215 None:intergenic
GCACTAACCTTATATAAAGA+AGG 0.507798 6_3:+20394023 None:intergenic
CTCCATGATTATGAAGGGCC+TGG 0.512793 6_3:-20393233 Msa0944020:CDS
GGAACATGGTTCATTGATGT+TGG 0.518756 6_3:-20393321 Msa0944020:CDS
CATATGGATGAAAGATTGAT+GGG 0.518975 6_3:-20393275 Msa0944020:CDS
GAACATAACCCAAAATCCCC+TGG 0.526511 6_3:-20393200 Msa0944020:CDS
AACCCAAAATCCCCTGGGAG+TGG 0.548305 6_3:-20393194 Msa0944020:CDS
TTTGCACATCAACCACTCCC+AGG 0.556615 6_3:+20393182 None:intergenic
TGGAACTCCATGATTATGAA+GGG 0.574524 6_3:-20393238 Msa0944020:CDS
TTGCACATCAACCACTCCCA+GGG 0.587694 6_3:+20393183 None:intergenic
AACATAACCCAAAATCCCCT+GGG 0.609618 6_3:-20393199 Msa0944020:CDS
TTTCATCTTGAATTGGATGG+TGG 0.617406 6_3:+20394246 None:intergenic
AATTAGAATCAAAGTATGTG+TGG 0.621826 6_3:-20393088 Msa0944020:three_prime_UTR
TTGCAGGGATAGCAAAGACA+TGG 0.624053 6_3:+20394173 None:intergenic
TGCACATCAACCACTCCCAG+GGG 0.679834 6_3:+20393184 None:intergenic
TGGATGGTGGAAGATAACAA+AGG 0.717266 6_3:+20394259 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATAAAAATATTAGTGTTT+TGG - chr6_3:20394216-20394235 Msa0944020:CDS 10.0%
!!! TAATAAAAATATTAGTGTTT+TGG - chr6_3:20394216-20394235 Msa0944020:CDS 10.0%
!!! ATGTATTGAATTGATTTTTT+TGG - chr6_3:20393112-20393131 Msa0944020:three_prime_UTR 15.0%
!!! TTTACTTTTCAAATTTTTGT+TGG - chr6_3:20393455-20393474 Msa0944020:intron 15.0%
!!! TTTTTTATTCTTTTAAATCG+TGG + chr6_3:20393589-20393608 None:intergenic 15.0%
!! TGAATTTGTAATATCAAATA+AGG - chr6_3:20394173-20394192 Msa0944020:CDS 15.0%
!!! ATGTATTGAATTGATTTTTT+TGG - chr6_3:20393112-20393131 Msa0944020:three_prime_UTR 15.0%
!!! TTTACTTTTCAAATTTTTGT+TGG - chr6_3:20393455-20393474 Msa0944020:intron 15.0%
!!! TTTTTTATTCTTTTAAATCG+TGG + chr6_3:20393589-20393608 None:intergenic 15.0%
!! TGAATTTGTAATATCAAATA+AGG - chr6_3:20394173-20394192 Msa0944020:CDS 15.0%
!!! AGTTTTTTTTCATCTTGAAT+TGG + chr6_3:20392966-20392985 None:intergenic 20.0%
!!! AGTTTTTCCTTCTTTATATA+AGG - chr6_3:20393172-20393191 Msa0944020:exon 20.0%
!!! CTTCTTAATTTTCATCTATT+TGG - chr6_3:20393201-20393220 Msa0944020:CDS 20.0%
!!! ATTTTTTCATCCAATATCTA+AGG - chr6_3:20393266-20393285 Msa0944020:CDS 20.0%
!!! AACTTTTTTGGCAAAAATTA+AGG + chr6_3:20393804-20393823 None:intergenic 20.0%
!!! GTTAATTAGTCAAACTTTTT+TGG + chr6_3:20393816-20393835 None:intergenic 20.0%
!!! AAAAACTAATTAGTTTGAAC+TGG - chr6_3:20394250-20394269 Msa0944020:CDS 20.0%
!!! AGTTTTTTTTCATCTTGAAT+TGG + chr6_3:20392966-20392985 None:intergenic 20.0%
!!! AGTTTTTCCTTCTTTATATA+AGG - chr6_3:20393172-20393191 Msa0944020:exon 20.0%
!!! CTTCTTAATTTTCATCTATT+TGG - chr6_3:20393201-20393220 Msa0944020:CDS 20.0%
!!! ATTTTTTCATCCAATATCTA+AGG - chr6_3:20393266-20393285 Msa0944020:CDS 20.0%
!!! AACTTTTTTGGCAAAAATTA+AGG + chr6_3:20393804-20393823 None:intergenic 20.0%
!!! GTTAATTAGTCAAACTTTTT+TGG + chr6_3:20393816-20393835 None:intergenic 20.0%
!!! AAAAACTAATTAGTTTGAAC+TGG - chr6_3:20394250-20394269 Msa0944020:CDS 20.0%
! CCACTAACATATTAATATTC+TGG + chr6_3:20392891-20392910 None:intergenic 25.0%
! CCAGAATATTAATATGTTAG+TGG - chr6_3:20392888-20392907 Msa0944020:three_prime_UTR 25.0%
!!! TTTTTTCATCTTGAATTGGA+TGG + chr6_3:20392962-20392981 None:intergenic 25.0%
!!! GAATTGATTTTTTTGGTTGT+AGG - chr6_3:20393119-20393138 Msa0944020:three_prime_UTR 25.0%
!! ATCTTCACCATTCAAATTTT+TGG + chr6_3:20393147-20393166 None:intergenic 25.0%
!! AAAGTCATGGTTATGTATTT+TGG - chr6_3:20393241-20393260 Msa0944020:CDS 25.0%
!! TCATTATTTGTGTGTTTTGT+AGG - chr6_3:20393860-20393879 Msa0944020:intron 25.0%
! AATTAGAATCAAAGTATGTG+TGG - chr6_3:20394114-20394133 Msa0944020:intron 25.0%
! CCACTAACATATTAATATTC+TGG + chr6_3:20392891-20392910 None:intergenic 25.0%
! CCAGAATATTAATATGTTAG+TGG - chr6_3:20392888-20392907 Msa0944020:three_prime_UTR 25.0%
!!! TTTTTTCATCTTGAATTGGA+TGG + chr6_3:20392962-20392981 None:intergenic 25.0%
!!! GAATTGATTTTTTTGGTTGT+AGG - chr6_3:20393119-20393138 Msa0944020:three_prime_UTR 25.0%
!! ATCTTCACCATTCAAATTTT+TGG + chr6_3:20393147-20393166 None:intergenic 25.0%
!! AAAGTCATGGTTATGTATTT+TGG - chr6_3:20393241-20393260 Msa0944020:CDS 25.0%
!! TCATTATTTGTGTGTTTTGT+AGG - chr6_3:20393860-20393879 Msa0944020:intron 25.0%
! AATTAGAATCAAAGTATGTG+TGG - chr6_3:20394114-20394133 Msa0944020:intron 25.0%
GTAGGAACCAAAAATTTGAA+TGG - chr6_3:20393137-20393156 Msa0944020:three_prime_UTR 30.0%
GCACTAACCTTATATAAAGA+AGG + chr6_3:20393182-20393201 None:intergenic 30.0%
!! CCTCATTTTTCCTTAGATAT+TGG + chr6_3:20393279-20393298 None:intergenic 30.0%
CCAATATCTAAGGAAAAATG+AGG - chr6_3:20393276-20393295 Msa0944020:CDS 30.0%
GGAAAAAAGTGAGAAAATGA+TGG - chr6_3:20393902-20393921 Msa0944020:intron 30.0%
TGAGAAAATGATGGTACATA+TGG - chr6_3:20393911-20393930 Msa0944020:intron 30.0%
ACATATGGATGAAAGATTGA+TGG - chr6_3:20393926-20393945 Msa0944020:intron 30.0%
CATATGGATGAAAGATTGAT+GGG - chr6_3:20393927-20393946 Msa0944020:intron 30.0%
! TTATGTTCTTTATTTGCACC+AGG + chr6_3:20393990-20394009 None:intergenic 30.0%
!!! GATTTTGCCTTCTAAAGTTT+AGG + chr6_3:20394069-20394088 None:intergenic 30.0%
!!! TGTGTGGTTTTTTGTTTCTT+TGG - chr6_3:20394130-20394149 Msa0944020:intron 30.0%
GTAGGAACCAAAAATTTGAA+TGG - chr6_3:20393137-20393156 Msa0944020:three_prime_UTR 30.0%
GCACTAACCTTATATAAAGA+AGG + chr6_3:20393182-20393201 None:intergenic 30.0%
!! CCTCATTTTTCCTTAGATAT+TGG + chr6_3:20393279-20393298 None:intergenic 30.0%
CCAATATCTAAGGAAAAATG+AGG - chr6_3:20393276-20393295 Msa0944020:CDS 30.0%
GGAAAAAAGTGAGAAAATGA+TGG - chr6_3:20393902-20393921 Msa0944020:intron 30.0%
TGAGAAAATGATGGTACATA+TGG - chr6_3:20393911-20393930 Msa0944020:intron 30.0%
ACATATGGATGAAAGATTGA+TGG - chr6_3:20393926-20393945 Msa0944020:intron 30.0%
CATATGGATGAAAGATTGAT+GGG - chr6_3:20393927-20393946 Msa0944020:intron 30.0%
! TTATGTTCTTTATTTGCACC+AGG + chr6_3:20393990-20394009 None:intergenic 30.0%
!!! GATTTTGCCTTCTAAAGTTT+AGG + chr6_3:20394069-20394088 None:intergenic 30.0%
!!! TGTGTGGTTTTTTGTTTCTT+TGG - chr6_3:20394130-20394149 Msa0944020:intron 30.0%
ATATTCTGGCGTACTATACT+AGG + chr6_3:20392877-20392896 None:intergenic 35.0%
TTTCATCTTGAATTGGATGG+TGG + chr6_3:20392959-20392978 None:intergenic 35.0%
! CAAAAACCTTACTTGTTGCA+GGG + chr6_3:20393047-20393066 None:intergenic 35.0%
! TCAAAAACCTTACTTGTTGC+AGG + chr6_3:20393048-20393067 None:intergenic 35.0%
TTCCTATTCTTGCAAAGTCA+TGG - chr6_3:20393228-20393247 Msa0944020:CDS 35.0%
AACCATGACTTTGCAAGAAT+AGG + chr6_3:20393233-20393252 None:intergenic 35.0%
! TTGTGTGTTTTGTAGGAACA+TGG - chr6_3:20393867-20393886 Msa0944020:intron 35.0%
ATGAAAGATTGATGGGAAGA+AGG - chr6_3:20393934-20393953 Msa0944020:intron 35.0%
TGAAAGATTGATGGGAAGAA+GGG - chr6_3:20393935-20393954 Msa0944020:intron 35.0%
TTGGAACTCCATGATTATGA+AGG - chr6_3:20393963-20393982 Msa0944020:intron 35.0%
TGGAACTCCATGATTATGAA+GGG - chr6_3:20393964-20393983 Msa0944020:intron 35.0%
GCTACATCCTAAACTTTAGA+AGG - chr6_3:20394059-20394078 Msa0944020:CDS 35.0%
ATATTCTGGCGTACTATACT+AGG + chr6_3:20392877-20392896 None:intergenic 35.0%
TTTCATCTTGAATTGGATGG+TGG + chr6_3:20392959-20392978 None:intergenic 35.0%
! CAAAAACCTTACTTGTTGCA+GGG + chr6_3:20393047-20393066 None:intergenic 35.0%
! TCAAAAACCTTACTTGTTGC+AGG + chr6_3:20393048-20393067 None:intergenic 35.0%
TTCCTATTCTTGCAAAGTCA+TGG - chr6_3:20393228-20393247 Msa0944020:CDS 35.0%
AACCATGACTTTGCAAGAAT+AGG + chr6_3:20393233-20393252 None:intergenic 35.0%
! TTGTGTGTTTTGTAGGAACA+TGG - chr6_3:20393867-20393886 Msa0944020:intron 35.0%
ATGAAAGATTGATGGGAAGA+AGG - chr6_3:20393934-20393953 Msa0944020:intron 35.0%
TGAAAGATTGATGGGAAGAA+GGG - chr6_3:20393935-20393954 Msa0944020:intron 35.0%
TTGGAACTCCATGATTATGA+AGG - chr6_3:20393963-20393982 Msa0944020:intron 35.0%
TGGAACTCCATGATTATGAA+GGG - chr6_3:20393964-20393983 Msa0944020:intron 35.0%
GCTACATCCTAAACTTTAGA+AGG - chr6_3:20394059-20394078 Msa0944020:CDS 35.0%
!! TGGATGGTGGAAGATAACAA+AGG + chr6_3:20392946-20392965 None:intergenic 40.0%
CAACAATTGTGTCAAGCAGA+AGG - chr6_3:20393409-20393428 Msa0944020:intron 40.0%
GGAACATGGTTCATTGATGT+TGG - chr6_3:20393881-20393900 Msa0944020:intron 40.0%
AACATAACCCAAAATCCCCT+GGG - chr6_3:20394003-20394022 Msa0944020:intron 40.0%
!! TGGATGGTGGAAGATAACAA+AGG + chr6_3:20392946-20392965 None:intergenic 40.0%
CAACAATTGTGTCAAGCAGA+AGG - chr6_3:20393409-20393428 Msa0944020:intron 40.0%
GGAACATGGTTCATTGATGT+TGG - chr6_3:20393881-20393900 Msa0944020:intron 40.0%
AACATAACCCAAAATCCCCT+GGG - chr6_3:20394003-20394022 Msa0944020:intron 40.0%
TTGCAGGGATAGCAAAGACA+TGG + chr6_3:20393032-20393051 None:intergenic 45.0%
TGCTATCCCTGCAACAAGTA+AGG - chr6_3:20393038-20393057 Msa0944020:three_prime_UTR 45.0%
GATGGGAAGAAGGGTCAATT+TGG - chr6_3:20393944-20393963 Msa0944020:intron 45.0%
GAACATAACCCAAAATCCCC+TGG - chr6_3:20394002-20394021 Msa0944020:intron 45.0%
TTGCAGGGATAGCAAAGACA+TGG + chr6_3:20393032-20393051 None:intergenic 45.0%
TGCTATCCCTGCAACAAGTA+AGG - chr6_3:20393038-20393057 Msa0944020:three_prime_UTR 45.0%
GATGGGAAGAAGGGTCAATT+TGG - chr6_3:20393944-20393963 Msa0944020:intron 45.0%
GAACATAACCCAAAATCCCC+TGG - chr6_3:20394002-20394021 Msa0944020:intron 45.0%
!!! ATTAACTTTTTAATTTATTT+TGG - chr6_3:20393830-20393849 Msa0944020:intron 5.0%
!!! ATTAACTTTTTAATTTATTT+TGG - chr6_3:20393830-20393849 Msa0944020:intron 5.0%
CTCCATGATTATGAAGGGCC+TGG - chr6_3:20393969-20393988 Msa0944020:intron 50.0%
CACCAGGCCCTTCATAATCA+TGG + chr6_3:20393974-20393993 None:intergenic 50.0%
! AACCACTCCCAGGGGATTTT+GGG + chr6_3:20394013-20394032 None:intergenic 50.0%
TTGCACATCAACCACTCCCA+GGG + chr6_3:20394022-20394041 None:intergenic 50.0%
TTTGCACATCAACCACTCCC+AGG + chr6_3:20394023-20394042 None:intergenic 50.0%
CTCCATGATTATGAAGGGCC+TGG - chr6_3:20393969-20393988 Msa0944020:intron 50.0%
CACCAGGCCCTTCATAATCA+TGG + chr6_3:20393974-20393993 None:intergenic 50.0%
! AACCACTCCCAGGGGATTTT+GGG + chr6_3:20394013-20394032 None:intergenic 50.0%
TTGCACATCAACCACTCCCA+GGG + chr6_3:20394022-20394041 None:intergenic 50.0%
TTTGCACATCAACCACTCCC+AGG + chr6_3:20394023-20394042 None:intergenic 50.0%
AACCCAAAATCCCCTGGGAG+TGG - chr6_3:20394008-20394027 Msa0944020:intron 55.0%
! CAACCACTCCCAGGGGATTT+TGG + chr6_3:20394014-20394033 None:intergenic 55.0%
TGCACATCAACCACTCCCAG+GGG + chr6_3:20394021-20394040 None:intergenic 55.0%
AACCCAAAATCCCCTGGGAG+TGG - chr6_3:20394008-20394027 Msa0944020:intron 55.0%
! CAACCACTCCCAGGGGATTT+TGG + chr6_3:20394014-20394033 None:intergenic 55.0%
TGCACATCAACCACTCCCAG+GGG + chr6_3:20394021-20394040 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 20392845 20394379 20392845 ID=Msa0944020;Name=Msa0944020
chr6_3 mRNA 20392845 20394379 20392845 ID=Msa0944020-mRNA-1;Parent=Msa0944020;Name=Msa0944020-mRNA-1;_AED=0.00;_eAED=0.00;_QI=56|1|1|1|1|1|3|345|119
chr6_3 exon 20392845 20393342 20392845 ID=Msa0944020-mRNA-1:exon:6084;Parent=Msa0944020-mRNA-1
chr6_3 exon 20394031 20394083 20394031 ID=Msa0944020-mRNA-1:exon:6083;Parent=Msa0944020-mRNA-1
chr6_3 exon 20394170 20394379 20394170 ID=Msa0944020-mRNA-1:exon:6082;Parent=Msa0944020-mRNA-1
chr6_3 five_prime_UTR 20394324 20394379 20394324 ID=Msa0944020-mRNA-1:five_prime_utr;Parent=Msa0944020-mRNA-1
chr6_3 CDS 20394170 20394323 20394170 ID=Msa0944020-mRNA-1:cds;Parent=Msa0944020-mRNA-1
chr6_3 CDS 20394031 20394083 20394031 ID=Msa0944020-mRNA-1:cds;Parent=Msa0944020-mRNA-1
chr6_3 CDS 20393190 20393342 20393190 ID=Msa0944020-mRNA-1:cds;Parent=Msa0944020-mRNA-1
chr6_3 three_prime_UTR 20392845 20393189 20392845 ID=Msa0944020-mRNA-1:three_prime_utr;Parent=Msa0944020-mRNA-1
Gene Sequence

>Msa0944020

ATGTTAGTGGCACTATATAACAAGCATAAAGTATTCATACATCCTTTGTTATCTTCCACCATCCAATTCAAGATGAAAAAAAACTTTTTCATACTTTTTGTGATACTTCTGAGTTTCTCATATATTCTCCATGTCTTTGCTATCCCTGCAACAAGAACCAAAAATTTGAATGGTGAAGATGAATCAGTTTTTCCTTCTTTATATAAGGAACATGGTTCATTGATGTTGGAAAAAAGTGAGAAAATGATGGTACATATGGATGAAAGATTGATGGGAAGAAGGGTCAATTTGGAACTCCATGATTATGAAGGGCCTGGTGCAAATAAAGAACATAACCCAAAATCCCCTGGGAGTGGTTGA

Protein sequence

>Msa0944020

MLVALYNKHKVFIHPLLSSTIQFKMKKNFFILFVILLSFSYILHVFAIPATRTKNLNGEDESVFPSLYKEHGSLMLEKSEKMMVHMDERLMGRRVNLELHDYEGPGANKEHNPKSPGSG*