AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1207950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1207950 MtrunA17_Chr4g0056921 100.000 151 0 0 1 151 1 151 5.58e-109 306
Msa1207950 MtrunA17_Chr4g0011261 94.040 151 9 0 1 151 1 151 2.54e-102 289
Msa1207950 MtrunA17_Chr5g0398801 92.715 151 11 0 1 151 1 151 1.19e-100 285
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1207950 AT4G00100.1 92.053 151 12 0 1 151 1 151 7.99e-101 286
Msa1207950 AT3G60770.1 92.053 151 12 0 1 151 1 151 1.99e-100 285

Find 62 sgRNAs with CRISPR-Local

Find 273 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAGATCGGTGTTATTCTT+AGG 0.199752 8_1:+65796608 Msa1207950:CDS
CCAGACTGGTGGGAGCTTCT+TGG 0.208703 8_1:-65797452 None:intergenic
TGCTGTTCTGCAGTCGCTTT+TGG 0.209015 8_1:+65798198 Msa1207950:three_prime_UTR
ATCCTTCAGATGCAGTAAAA+TGG 0.243022 8_1:-65798419 None:intergenic
AAGCTGCGAGCTTCGTGATT+AGG 0.287127 8_1:-65796197 None:intergenic
CATCTGAAGGATACATTGTT+TGG 0.295241 8_1:+65798430 Msa1207950:three_prime_UTR
GCTCAAGTCAAGTTTATAAC+TGG 0.327822 8_1:+65796646 Msa1207950:CDS
TTAACTAGTTCAAGTCTTCC+AGG 0.332262 8_1:-65798266 None:intergenic
AGCTGCGAGCTTCGTGATTA+GGG 0.339227 8_1:-65796196 None:intergenic
TATATACATGCTGGAAATCT+TGG 0.346414 8_1:+65798389 Msa1207950:three_prime_UTR
TCAAGAAGGCAGTTTCAATT+AGG 0.353735 8_1:+65797332 Msa1207950:CDS
TACAGATCCTCAGGAATTTC+CGG 0.365553 8_1:-65797301 None:intergenic
CACAAGAATCAACCTGAACT+TGG 0.390376 8_1:-65797389 None:intergenic
TGTTGATGTTGCGTTGGGTT+TGG 0.400753 8_1:+65798470 Msa1207950:three_prime_UTR
TCAGATCGGTGTTATTCTTA+GGG 0.403625 8_1:+65796609 Msa1207950:CDS
AGTTTCAATTAGGAAGCATT+TGG 0.418745 8_1:+65797342 Msa1207950:CDS
TATTTGCAAGTTTGCAAAGA+AGG 0.423456 8_1:+65796573 Msa1207950:CDS
CCAAGAAGCTCCCACCAGTC+TGG 0.424976 8_1:+65797452 Msa1207950:CDS
TCCTTCAGATGCAGTAAAAT+GGG 0.425309 8_1:-65798418 None:intergenic
AAACTGCCTTCTTGATCAAA+TGG 0.434219 8_1:-65797324 None:intergenic
GTTGCGTTGGGTTTGGACTT+TGG 0.441412 8_1:+65798477 Msa1207950:three_prime_UTR
AGGATAAGGACTCCAAGTTC+AGG 0.449803 8_1:+65797377 Msa1207950:CDS
AGTGAACCAGAACTAGGGTT+TGG 0.452696 8_1:+65796139 None:intergenic
TTTGGTGTCATGATCAGTCC+TGG 0.466230 8_1:+65798248 Msa1207950:three_prime_UTR
TAACTGGCAGCAAAATCCTT+AGG 0.477614 8_1:+65796662 Msa1207950:CDS
ATCAAATGGTACAGATCCTC+AGG 0.478375 8_1:-65797310 None:intergenic
GGAGAGGAACAGAAAGGATA+AGG 0.479750 8_1:+65797363 Msa1207950:CDS
CCTGGTGCAGACCTCTTGTA+TGG 0.493479 8_1:-65796407 None:intergenic
CAAGTTCAGGTTGATTCTTG+TGG 0.500791 8_1:+65797390 Msa1207950:CDS
GCTTAAAGCTTCAGCAACCA+TGG 0.503394 8_1:+65796216 Msa1207950:exon
GTTATTCTTAGGGATTCTCA+TGG 0.504564 8_1:+65796619 Msa1207950:CDS
ATTTGCAAGTTTGCAAAGAA+GGG 0.505113 8_1:+65796574 Msa1207950:CDS
CGTGAGCCTTGAGGATCCTA+AGG 0.518888 8_1:-65796678 None:intergenic
ATCAACAACTGCCAGCACTT+TGG 0.526669 8_1:+65798142 Msa1207950:CDS
CTTAAAGCTTCAGCAACCAT+GGG 0.528681 8_1:+65796217 Msa1207950:exon
GTAGAGGTCTTGAGCCATCC+TGG 0.536330 8_1:-65796425 None:intergenic
AAACATGTTGATGTTGCGTT+GGG 0.538051 8_1:+65798465 Msa1207950:three_prime_UTR
GCCACTGTGCATACGCCCCA+TGG 0.542099 8_1:-65796233 None:intergenic
AGCATAACAAGTCAAAATCA+AGG 0.544124 8_1:-65798179 None:intergenic
AGAATAACACCGATCTGAGA+TGG 0.551609 8_1:-65796604 None:intergenic
TCTCTAAGCAACCAAAGTGC+TGG 0.564231 8_1:-65798153 None:intergenic
TGGGGCGTATGCACAGTGGC+GGG 0.570294 8_1:+65796236 Msa1207950:CDS
AAAACATGTTGATGTTGCGT+TGG 0.577507 8_1:+65798464 Msa1207950:three_prime_UTR
CCATACAAGAGGTCTGCACC+AGG 0.584958 8_1:+65796407 Msa1207950:CDS
TCTGTACCATTTGATCAAGA+AGG 0.592710 8_1:+65797318 Msa1207950:CDS
CTTCTGCTTTACCATACAAG+AGG 0.597371 8_1:+65796396 Msa1207950:CDS
ATGGGGCGTATGCACAGTGG+CGG 0.600106 8_1:+65796235 Msa1207950:CDS
AGTACTTACTACTTCCAGAC+TGG 0.601291 8_1:-65797466 None:intergenic
ACTTACTACTTCCAGACTGG+TGG 0.603353 8_1:-65797463 None:intergenic
CAATTAGGAAGCATTTGGAG+AGG 0.603553 8_1:+65797347 Msa1207950:CDS
CTTAGGATCCTCAAGGCTCA+CGG 0.606181 8_1:+65796679 Msa1207950:CDS
GCATTTGGAGAGGAACAGAA+AGG 0.626868 8_1:+65797357 Msa1207950:CDS
ACAAGAGGTCTGCACCAGGA+TGG 0.633754 8_1:+65796411 Msa1207950:CDS
CAAAATCCTTAGGATCCTCA+AGG 0.639899 8_1:+65796672 Msa1207950:CDS
AGAACATACCGTGAGCCTTG+AGG 0.640882 8_1:-65796687 None:intergenic
CTGTTGTCATTTGAAAGATG+TGG 0.649779 8_1:+65798335 Msa1207950:three_prime_UTR
CTTACTACTTCCAGACTGGT+GGG 0.667382 8_1:-65797462 None:intergenic
GGTTTGACTCCATCTCAGAT+CGG 0.677869 8_1:+65796595 Msa1207950:CDS
TCTTGTAATAGCGAGCAAGA+CGG 0.689009 8_1:-65797426 None:intergenic
ACTTGCACCGGAAATTCCTG+AGG 0.709274 8_1:+65797294 Msa1207950:CDS
TTAAAGCTTCAGCAACCATG+GGG 0.724366 8_1:+65796218 Msa1207950:exon
ACCATGGGGCGTATGCACAG+TGG 0.730613 8_1:+65796232 Msa1207950:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTATATATCTAATAATATAA+TGG + chr8_1:65797862-65797881 Msa1207950:intron 10.0%
!! CTATATATCTAATAATATAA+TGG + chr8_1:65797862-65797881 Msa1207950:intron 10.0%
!!! TTTTGAATTGTTTCAAATTT+TGG + chr8_1:65796353-65796372 Msa1207950:intron 15.0%
!! CAATAACAAAAAATGATAAT+GGG - chr8_1:65796473-65796492 None:intergenic 15.0%
!! ACAATAACAAAAAATGATAA+TGG - chr8_1:65796474-65796493 None:intergenic 15.0%
!!! TTTTTAATTTGAATTATAGG+TGG + chr8_1:65796543-65796562 Msa1207950:intron 15.0%
!! ATACATAAAAGAAGAAAAAA+TGG - chr8_1:65797568-65797587 None:intergenic 15.0%
!! CAAAAAAAAATACAAGATAT+TGG - chr8_1:65798071-65798090 None:intergenic 15.0%
!!! CAATATCTTGTATTTTTTTT+TGG + chr8_1:65798069-65798088 Msa1207950:intron 15.0%
!!! TTTTGAATTGTTTCAAATTT+TGG + chr8_1:65796353-65796372 Msa1207950:intron 15.0%
!! CAATAACAAAAAATGATAAT+GGG - chr8_1:65796473-65796492 None:intergenic 15.0%
!! ACAATAACAAAAAATGATAA+TGG - chr8_1:65796474-65796493 None:intergenic 15.0%
!!! TTTTTAATTTGAATTATAGG+TGG + chr8_1:65796543-65796562 Msa1207950:intron 15.0%
!! ATACATAAAAGAAGAAAAAA+TGG - chr8_1:65797568-65797587 None:intergenic 15.0%
!! CAAAAAAAAATACAAGATAT+TGG - chr8_1:65798071-65798090 None:intergenic 15.0%
!!! CAATATCTTGTATTTTTTTT+TGG + chr8_1:65798069-65798088 Msa1207950:intron 15.0%
!! ACAATTCAAAACAAAAACAT+TGG - chr8_1:65796344-65796363 None:intergenic 20.0%
!!! ATTTGTGTCATTATAGATTA+TGG + chr8_1:65796734-65796753 Msa1207950:intron 20.0%
!!! TTTGTGTCATTATAGATTAT+GGG + chr8_1:65796735-65796754 Msa1207950:intron 20.0%
!!! TTTTCAGTTTATTTGCATAT+GGG + chr8_1:65796860-65796879 Msa1207950:intron 20.0%
!! TTATGTGAATTTAGATAAAC+AGG + chr8_1:65797081-65797100 Msa1207950:intron 20.0%
!! ATATTATAAGCACTTATGAT+AGG + chr8_1:65797186-65797205 Msa1207950:intron 20.0%
!!! TAGTAAATGAGATTTCTTTT+GGG + chr8_1:65797259-65797278 Msa1207950:intron 20.0%
!! AGATTATTTGATCTATTTGA+AGG + chr8_1:65797632-65797651 Msa1207950:intron 20.0%
!! ACATTTAATACAATAGCATA+GGG - chr8_1:65797669-65797688 None:intergenic 20.0%
!! TTAAATGTCAATCTATTTGA+AGG + chr8_1:65797681-65797700 Msa1207950:intron 20.0%
!!! TATATAGCTTTTGTTGAAAA+TGG - chr8_1:65797849-65797868 None:intergenic 20.0%
!!! TATAATGTTATTGCTTTTGT+CGG + chr8_1:65798038-65798057 Msa1207950:intron 20.0%
!!! ATTTTAAGTTATATACATGC+TGG + chr8_1:65798380-65798399 Msa1207950:three_prime_UTR 20.0%
!! ACAATTCAAAACAAAAACAT+TGG - chr8_1:65796344-65796363 None:intergenic 20.0%
!!! ATTTGTGTCATTATAGATTA+TGG + chr8_1:65796734-65796753 Msa1207950:intron 20.0%
!!! TTTGTGTCATTATAGATTAT+GGG + chr8_1:65796735-65796754 Msa1207950:intron 20.0%
!!! TTTTCAGTTTATTTGCATAT+GGG + chr8_1:65796860-65796879 Msa1207950:intron 20.0%
!! TTATGTGAATTTAGATAAAC+AGG + chr8_1:65797081-65797100 Msa1207950:intron 20.0%
!! ATATTATAAGCACTTATGAT+AGG + chr8_1:65797186-65797205 Msa1207950:intron 20.0%
!!! TAGTAAATGAGATTTCTTTT+GGG + chr8_1:65797259-65797278 Msa1207950:intron 20.0%
!! AGATTATTTGATCTATTTGA+AGG + chr8_1:65797632-65797651 Msa1207950:intron 20.0%
!! ACATTTAATACAATAGCATA+GGG - chr8_1:65797669-65797688 None:intergenic 20.0%
!! TTAAATGTCAATCTATTTGA+AGG + chr8_1:65797681-65797700 Msa1207950:intron 20.0%
!!! TATATAGCTTTTGTTGAAAA+TGG - chr8_1:65797849-65797868 None:intergenic 20.0%
!!! TATAATGTTATTGCTTTTGT+CGG + chr8_1:65798038-65798057 Msa1207950:intron 20.0%
!!! ATTTTAAGTTATATACATGC+TGG + chr8_1:65798380-65798399 Msa1207950:three_prime_UTR 20.0%
! TTTCACAAAAATCATCGTTT+CGG + chr8_1:65796163-65796182 Msa1207950:five_prime_UTR 25.0%
!!! TTCAAATTTTGGTTACAGTA+AGG + chr8_1:65796364-65796383 Msa1207950:intron 25.0%
!!! TCAAATTTTGGTTACAGTAA+GGG + chr8_1:65796365-65796384 Msa1207950:intron 25.0%
! AAAAATTACATCTTGTGTAG+AGG - chr8_1:65796444-65796463 None:intergenic 25.0%
! CTACCAAATAAAGTTCTTAT+GGG - chr8_1:65796775-65796794 None:intergenic 25.0%
! ACTACCAAATAAAGTTCTTA+TGG - chr8_1:65796776-65796795 None:intergenic 25.0%
!! GTTTTCAGTTTATTTGCATA+TGG + chr8_1:65796859-65796878 Msa1207950:intron 25.0%
! CTTATGAAAACAGGTTATAT+TGG + chr8_1:65797109-65797128 Msa1207950:intron 25.0%
!! GTAGTAAATGAGATTTCTTT+TGG + chr8_1:65797258-65797277 Msa1207950:intron 25.0%
!! AGTAAATGAGATTTCTTTTG+GGG + chr8_1:65797260-65797279 Msa1207950:intron 25.0%
!! TAGTACTAGAAAAAGGATTT+TGG + chr8_1:65797540-65797559 Msa1207950:intron 25.0%
! ATTTAATACAATAGCATAGG+GGG - chr8_1:65797667-65797686 None:intergenic 25.0%
! CATTTAATACAATAGCATAG+GGG - chr8_1:65797668-65797687 None:intergenic 25.0%
! GACATTTAATACAATAGCAT+AGG - chr8_1:65797670-65797689 None:intergenic 25.0%
!! CATTTTTCTACTCTTTCTAT+TGG + chr8_1:65797745-65797764 Msa1207950:intron 25.0%
! GTTGTATAAAACAAAGTTTC+AGG + chr8_1:65797779-65797798 Msa1207950:intron 25.0%
! GTTATGTTGAAATTCTATTC+TGG + chr8_1:65797930-65797949 Msa1207950:intron 25.0%
! GACAAAAGCAATAACATTAT+AGG - chr8_1:65798039-65798058 None:intergenic 25.0%
! AGTTCTACAACTGATAAAAA+AGG - chr8_1:65798313-65798332 None:intergenic 25.0%
!!! ATACATTGTTTGGTTTTTGA+AGG + chr8_1:65798440-65798459 Msa1207950:three_prime_UTR 25.0%
! CCAAATTAAAATAGTTCAAC+AGG + chr8_1:65798525-65798544 Msa1207950:three_prime_UTR 25.0%
! TTTCACAAAAATCATCGTTT+CGG + chr8_1:65796163-65796182 Msa1207950:five_prime_UTR 25.0%
!!! TTCAAATTTTGGTTACAGTA+AGG + chr8_1:65796364-65796383 Msa1207950:intron 25.0%
!!! TCAAATTTTGGTTACAGTAA+GGG + chr8_1:65796365-65796384 Msa1207950:intron 25.0%
! AAAAATTACATCTTGTGTAG+AGG - chr8_1:65796444-65796463 None:intergenic 25.0%
! CTACCAAATAAAGTTCTTAT+GGG - chr8_1:65796775-65796794 None:intergenic 25.0%
! ACTACCAAATAAAGTTCTTA+TGG - chr8_1:65796776-65796795 None:intergenic 25.0%
!! GTTTTCAGTTTATTTGCATA+TGG + chr8_1:65796859-65796878 Msa1207950:intron 25.0%
! CTTATGAAAACAGGTTATAT+TGG + chr8_1:65797109-65797128 Msa1207950:intron 25.0%
!! GTAGTAAATGAGATTTCTTT+TGG + chr8_1:65797258-65797277 Msa1207950:intron 25.0%
!! AGTAAATGAGATTTCTTTTG+GGG + chr8_1:65797260-65797279 Msa1207950:intron 25.0%
!! TAGTACTAGAAAAAGGATTT+TGG + chr8_1:65797540-65797559 Msa1207950:intron 25.0%
! ATTTAATACAATAGCATAGG+GGG - chr8_1:65797667-65797686 None:intergenic 25.0%
! CATTTAATACAATAGCATAG+GGG - chr8_1:65797668-65797687 None:intergenic 25.0%
! GACATTTAATACAATAGCAT+AGG - chr8_1:65797670-65797689 None:intergenic 25.0%
!! CATTTTTCTACTCTTTCTAT+TGG + chr8_1:65797745-65797764 Msa1207950:intron 25.0%
! GTTGTATAAAACAAAGTTTC+AGG + chr8_1:65797779-65797798 Msa1207950:intron 25.0%
! GTTATGTTGAAATTCTATTC+TGG + chr8_1:65797930-65797949 Msa1207950:intron 25.0%
! GACAAAAGCAATAACATTAT+AGG - chr8_1:65798039-65798058 None:intergenic 25.0%
! AGTTCTACAACTGATAAAAA+AGG - chr8_1:65798313-65798332 None:intergenic 25.0%
!!! ATACATTGTTTGGTTTTTGA+AGG + chr8_1:65798440-65798459 Msa1207950:three_prime_UTR 25.0%
TATTTGCAAGTTTGCAAAGA+AGG + chr8_1:65796573-65796592 Msa1207950:CDS 30.0%
ATTTGCAAGTTTGCAAAGAA+GGG + chr8_1:65796574-65796593 Msa1207950:CDS 30.0%
TGACCCATAAGAACTTTATT+TGG + chr8_1:65796769-65796788 Msa1207950:intron 30.0%
AAGCACTTGTAAAACTGATT+AGG + chr8_1:65796974-65796993 Msa1207950:intron 30.0%
AAGCACTTGTGAAATTGATT+AGG + chr8_1:65797030-65797049 Msa1207950:intron 30.0%
!!! TTTCTTTTGGGGTTGATTTT+AGG + chr8_1:65797271-65797290 Msa1207950:intron 30.0%
!! AGTTTCAATTAGGAAGCATT+TGG + chr8_1:65797342-65797361 Msa1207950:CDS 30.0%
! AAATTTTACACTTTGCACCT+CGG + chr8_1:65797718-65797737 Msa1207950:intron 30.0%
TTGTTGAAAATGGATATCCA+CGG - chr8_1:65797839-65797858 None:intergenic 30.0%
GCATGTTACATTTGATACTT+TGG + chr8_1:65797902-65797921 Msa1207950:intron 30.0%
TAACCTAAAATTCTAGCATC+AGG - chr8_1:65798002-65798021 None:intergenic 30.0%
AGAGACAAGTCATTAATATC+AGG - chr8_1:65798112-65798131 None:intergenic 30.0%
AGCATAACAAGTCAAAATCA+AGG - chr8_1:65798182-65798201 None:intergenic 30.0%
!!! TTGCTCGAATGTAGTTTTTT+TGG + chr8_1:65798230-65798249 Msa1207950:three_prime_UTR 30.0%
TATATACATGCTGGAAATCT+TGG + chr8_1:65798389-65798408 Msa1207950:three_prime_UTR 30.0%
TATTTGCAAGTTTGCAAAGA+AGG + chr8_1:65796573-65796592 Msa1207950:CDS 30.0%
ATTTGCAAGTTTGCAAAGAA+GGG + chr8_1:65796574-65796593 Msa1207950:CDS 30.0%
TGACCCATAAGAACTTTATT+TGG + chr8_1:65796769-65796788 Msa1207950:intron 30.0%
AAGCACTTGTAAAACTGATT+AGG + chr8_1:65796974-65796993 Msa1207950:intron 30.0%
AAGCACTTGTGAAATTGATT+AGG + chr8_1:65797030-65797049 Msa1207950:intron 30.0%
!!! TTTCTTTTGGGGTTGATTTT+AGG + chr8_1:65797271-65797290 Msa1207950:intron 30.0%
!! AGTTTCAATTAGGAAGCATT+TGG + chr8_1:65797342-65797361 Msa1207950:CDS 30.0%
! AAATTTTACACTTTGCACCT+CGG + chr8_1:65797718-65797737 Msa1207950:intron 30.0%
TTGTTGAAAATGGATATCCA+CGG - chr8_1:65797839-65797858 None:intergenic 30.0%
GCATGTTACATTTGATACTT+TGG + chr8_1:65797902-65797921 Msa1207950:intron 30.0%
TAACCTAAAATTCTAGCATC+AGG - chr8_1:65798002-65798021 None:intergenic 30.0%
AGAGACAAGTCATTAATATC+AGG - chr8_1:65798112-65798131 None:intergenic 30.0%
AGCATAACAAGTCAAAATCA+AGG - chr8_1:65798182-65798201 None:intergenic 30.0%
!!! TTGCTCGAATGTAGTTTTTT+TGG + chr8_1:65798230-65798249 Msa1207950:three_prime_UTR 30.0%
TATATACATGCTGGAAATCT+TGG + chr8_1:65798389-65798408 Msa1207950:three_prime_UTR 30.0%
TTGCGATTGAGATTGAGAAT+GGG - chr8_1:65796275-65796294 None:intergenic 35.0%
ATTGCGATTGAGATTGAGAA+TGG - chr8_1:65796276-65796295 None:intergenic 35.0%
!! TCAGATCGGTGTTATTCTTA+GGG + chr8_1:65796609-65796628 Msa1207950:CDS 35.0%
GTTATTCTTAGGGATTCTCA+TGG + chr8_1:65796619-65796638 Msa1207950:CDS 35.0%
GCTCAAGTCAAGTTTATAAC+TGG + chr8_1:65796646-65796665 Msa1207950:CDS 35.0%
ATCAATTTCACAAGTGCTTG+TGG - chr8_1:65797029-65797048 None:intergenic 35.0%
TCATAGAAACAGTGTATGAC+AGG + chr8_1:65797058-65797077 Msa1207950:intron 35.0%
TCTGTACCATTTGATCAAGA+AGG + chr8_1:65797318-65797337 Msa1207950:CDS 35.0%
AAACTGCCTTCTTGATCAAA+TGG - chr8_1:65797327-65797346 None:intergenic 35.0%
TCAAGAAGGCAGTTTCAATT+AGG + chr8_1:65797332-65797351 Msa1207950:CDS 35.0%
!! TCCTTTTTCTAGTACTACCA+CGG - chr8_1:65797537-65797556 None:intergenic 35.0%
TCACTTCACACATCAAAACA+TGG - chr8_1:65797612-65797631 None:intergenic 35.0%
! ATAAAGAAGCGCATCAAACA+AGG - chr8_1:65797958-65797977 None:intergenic 35.0%
!! ATGTTATTGCTTTTGTCGGT+TGG + chr8_1:65798042-65798061 Msa1207950:intron 35.0%
TTAACTAGTTCAAGTCTTCC+AGG - chr8_1:65798269-65798288 None:intergenic 35.0%
! CTGTTGTCATTTGAAAGATG+TGG + chr8_1:65798335-65798354 Msa1207950:three_prime_UTR 35.0%
TCCTTCAGATGCAGTAAAAT+GGG - chr8_1:65798421-65798440 None:intergenic 35.0%
ATCCTTCAGATGCAGTAAAA+TGG - chr8_1:65798422-65798441 None:intergenic 35.0%
CATCTGAAGGATACATTGTT+TGG + chr8_1:65798430-65798449 Msa1207950:three_prime_UTR 35.0%
AAAACATGTTGATGTTGCGT+TGG + chr8_1:65798464-65798483 Msa1207950:three_prime_UTR 35.0%
AAACATGTTGATGTTGCGTT+GGG + chr8_1:65798465-65798484 Msa1207950:three_prime_UTR 35.0%
TTGCGATTGAGATTGAGAAT+GGG - chr8_1:65796275-65796294 None:intergenic 35.0%
ATTGCGATTGAGATTGAGAA+TGG - chr8_1:65796276-65796295 None:intergenic 35.0%
!! TCAGATCGGTGTTATTCTTA+GGG + chr8_1:65796609-65796628 Msa1207950:CDS 35.0%
GTTATTCTTAGGGATTCTCA+TGG + chr8_1:65796619-65796638 Msa1207950:CDS 35.0%
GCTCAAGTCAAGTTTATAAC+TGG + chr8_1:65796646-65796665 Msa1207950:CDS 35.0%
ATCAATTTCACAAGTGCTTG+TGG - chr8_1:65797029-65797048 None:intergenic 35.0%
TCATAGAAACAGTGTATGAC+AGG + chr8_1:65797058-65797077 Msa1207950:intron 35.0%
TCTGTACCATTTGATCAAGA+AGG + chr8_1:65797318-65797337 Msa1207950:CDS 35.0%
AAACTGCCTTCTTGATCAAA+TGG - chr8_1:65797327-65797346 None:intergenic 35.0%
TCAAGAAGGCAGTTTCAATT+AGG + chr8_1:65797332-65797351 Msa1207950:CDS 35.0%
!! TCCTTTTTCTAGTACTACCA+CGG - chr8_1:65797537-65797556 None:intergenic 35.0%
TCACTTCACACATCAAAACA+TGG - chr8_1:65797612-65797631 None:intergenic 35.0%
! ATAAAGAAGCGCATCAAACA+AGG - chr8_1:65797958-65797977 None:intergenic 35.0%
!! ATGTTATTGCTTTTGTCGGT+TGG + chr8_1:65798042-65798061 Msa1207950:intron 35.0%
TTAACTAGTTCAAGTCTTCC+AGG - chr8_1:65798269-65798288 None:intergenic 35.0%
! CTGTTGTCATTTGAAAGATG+TGG + chr8_1:65798335-65798354 Msa1207950:three_prime_UTR 35.0%
TCCTTCAGATGCAGTAAAAT+GGG - chr8_1:65798421-65798440 None:intergenic 35.0%
ATCCTTCAGATGCAGTAAAA+TGG - chr8_1:65798422-65798441 None:intergenic 35.0%
CATCTGAAGGATACATTGTT+TGG + chr8_1:65798430-65798449 Msa1207950:three_prime_UTR 35.0%
AAAACATGTTGATGTTGCGT+TGG + chr8_1:65798464-65798483 Msa1207950:three_prime_UTR 35.0%
AAACATGTTGATGTTGCGTT+GGG + chr8_1:65798465-65798484 Msa1207950:three_prime_UTR 35.0%
CTTAAAGCTTCAGCAACCAT+GGG + chr8_1:65796217-65796236 Msa1207950:exon 40.0%
TTAAAGCTTCAGCAACCATG+GGG + chr8_1:65796218-65796237 Msa1207950:exon 40.0%
TTGAGATTGAGAATGGGGAA+TGG - chr8_1:65796269-65796288 None:intergenic 40.0%
TGCGATTGAGATTGAGAATG+GGG - chr8_1:65796274-65796293 None:intergenic 40.0%
CTTCTGCTTTACCATACAAG+AGG + chr8_1:65796396-65796415 Msa1207950:CDS 40.0%
AGAATAACACCGATCTGAGA+TGG - chr8_1:65796607-65796626 None:intergenic 40.0%
!! CTCAGATCGGTGTTATTCTT+AGG + chr8_1:65796608-65796627 Msa1207950:CDS 40.0%
TAACTGGCAGCAAAATCCTT+AGG + chr8_1:65796662-65796681 Msa1207950:CDS 40.0%
CAAAATCCTTAGGATCCTCA+AGG + chr8_1:65796672-65796691 Msa1207950:CDS 40.0%
TATTTGCATATGGGCATGTG+TGG + chr8_1:65796869-65796888 Msa1207950:intron 40.0%
CAGGCATAGCTTATGAAAAC+AGG + chr8_1:65797100-65797119 Msa1207950:intron 40.0%
!!! GTTGATTTTAGGACTTGCAC+CGG + chr8_1:65797282-65797301 Msa1207950:intron 40.0%
TACAGATCCTCAGGAATTTC+CGG - chr8_1:65797304-65797323 None:intergenic 40.0%
ATCAAATGGTACAGATCCTC+AGG - chr8_1:65797313-65797332 None:intergenic 40.0%
!! CAATTAGGAAGCATTTGGAG+AGG + chr8_1:65797347-65797366 Msa1207950:CDS 40.0%
CACAAGAATCAACCTGAACT+TGG - chr8_1:65797392-65797411 None:intergenic 40.0%
!! CAAGTTCAGGTTGATTCTTG+TGG + chr8_1:65797390-65797409 Msa1207950:CDS 40.0%
TCTTGTAATAGCGAGCAAGA+CGG - chr8_1:65797429-65797448 None:intergenic 40.0%
AGTACTTACTACTTCCAGAC+TGG - chr8_1:65797469-65797488 None:intergenic 40.0%
!! CCTTTTTCTAGTACTACCAC+GGG - chr8_1:65797536-65797555 None:intergenic 40.0%
CTAAAATTCTAGCATCAGGC+AGG - chr8_1:65797998-65798017 None:intergenic 40.0%
! CTGCCTGATGCTAGAATTTT+AGG + chr8_1:65797996-65798015 Msa1207950:intron 40.0%
!! ATGTGGCTGACTTCAGTTTT+TGG + chr8_1:65798352-65798371 Msa1207950:three_prime_UTR 40.0%
! ACCCATTTTACTGCATCTGA+AGG + chr8_1:65798417-65798436 Msa1207950:three_prime_UTR 40.0%
CTTAAAGCTTCAGCAACCAT+GGG + chr8_1:65796217-65796236 Msa1207950:exon 40.0%
TTAAAGCTTCAGCAACCATG+GGG + chr8_1:65796218-65796237 Msa1207950:exon 40.0%
TTGAGATTGAGAATGGGGAA+TGG - chr8_1:65796269-65796288 None:intergenic 40.0%
TGCGATTGAGATTGAGAATG+GGG - chr8_1:65796274-65796293 None:intergenic 40.0%
CTTCTGCTTTACCATACAAG+AGG + chr8_1:65796396-65796415 Msa1207950:CDS 40.0%
AGAATAACACCGATCTGAGA+TGG - chr8_1:65796607-65796626 None:intergenic 40.0%
!! CTCAGATCGGTGTTATTCTT+AGG + chr8_1:65796608-65796627 Msa1207950:CDS 40.0%
TAACTGGCAGCAAAATCCTT+AGG + chr8_1:65796662-65796681 Msa1207950:CDS 40.0%
CAAAATCCTTAGGATCCTCA+AGG + chr8_1:65796672-65796691 Msa1207950:CDS 40.0%
TATTTGCATATGGGCATGTG+TGG + chr8_1:65796869-65796888 Msa1207950:intron 40.0%
CAGGCATAGCTTATGAAAAC+AGG + chr8_1:65797100-65797119 Msa1207950:intron 40.0%
!!! GTTGATTTTAGGACTTGCAC+CGG + chr8_1:65797282-65797301 Msa1207950:intron 40.0%
TACAGATCCTCAGGAATTTC+CGG - chr8_1:65797304-65797323 None:intergenic 40.0%
ATCAAATGGTACAGATCCTC+AGG - chr8_1:65797313-65797332 None:intergenic 40.0%
!! CAATTAGGAAGCATTTGGAG+AGG + chr8_1:65797347-65797366 Msa1207950:CDS 40.0%
CACAAGAATCAACCTGAACT+TGG - chr8_1:65797392-65797411 None:intergenic 40.0%
!! CAAGTTCAGGTTGATTCTTG+TGG + chr8_1:65797390-65797409 Msa1207950:CDS 40.0%
TCTTGTAATAGCGAGCAAGA+CGG - chr8_1:65797429-65797448 None:intergenic 40.0%
AGTACTTACTACTTCCAGAC+TGG - chr8_1:65797469-65797488 None:intergenic 40.0%
!! CCTTTTTCTAGTACTACCAC+GGG - chr8_1:65797536-65797555 None:intergenic 40.0%
CTAAAATTCTAGCATCAGGC+AGG - chr8_1:65797998-65798017 None:intergenic 40.0%
! CTGCCTGATGCTAGAATTTT+AGG + chr8_1:65797996-65798015 Msa1207950:intron 40.0%
!! ATGTGGCTGACTTCAGTTTT+TGG + chr8_1:65798352-65798371 Msa1207950:three_prime_UTR 40.0%
! ACCCATTTTACTGCATCTGA+AGG + chr8_1:65798417-65798436 Msa1207950:three_prime_UTR 40.0%
GCTTAAAGCTTCAGCAACCA+TGG + chr8_1:65796216-65796235 Msa1207950:exon 45.0%
GGTTTGACTCCATCTCAGAT+CGG + chr8_1:65796595-65796614 Msa1207950:CDS 45.0%
GCATTTGGAGAGGAACAGAA+AGG + chr8_1:65797357-65797376 Msa1207950:CDS 45.0%
GGAGAGGAACAGAAAGGATA+AGG + chr8_1:65797363-65797382 Msa1207950:CDS 45.0%
AGGATAAGGACTCCAAGTTC+AGG + chr8_1:65797377-65797396 Msa1207950:CDS 45.0%
CTTACTACTTCCAGACTGGT+GGG - chr8_1:65797465-65797484 None:intergenic 45.0%
ACTTACTACTTCCAGACTGG+TGG - chr8_1:65797466-65797485 None:intergenic 45.0%
CCCGTGGTAGTACTAGAAAA+AGG + chr8_1:65797533-65797552 Msa1207950:intron 45.0%
GAGTAGAAAAATGTCTGCCG+AGG - chr8_1:65797738-65797757 None:intergenic 45.0%
TGTCTTTGTGTTTGCTTCCG+TGG + chr8_1:65797819-65797838 Msa1207950:intron 45.0%
ATCAACAACTGCCAGCACTT+TGG + chr8_1:65798142-65798161 Msa1207950:CDS 45.0%
TCTCTAAGCAACCAAAGTGC+TGG - chr8_1:65798156-65798175 None:intergenic 45.0%
!! TTTGGTGTCATGATCAGTCC+TGG + chr8_1:65798248-65798267 Msa1207950:three_prime_UTR 45.0%
TGTTGATGTTGCGTTGGGTT+TGG + chr8_1:65798470-65798489 Msa1207950:three_prime_UTR 45.0%
GCTTAAAGCTTCAGCAACCA+TGG + chr8_1:65796216-65796235 Msa1207950:exon 45.0%
GGTTTGACTCCATCTCAGAT+CGG + chr8_1:65796595-65796614 Msa1207950:CDS 45.0%
GCATTTGGAGAGGAACAGAA+AGG + chr8_1:65797357-65797376 Msa1207950:CDS 45.0%
GGAGAGGAACAGAAAGGATA+AGG + chr8_1:65797363-65797382 Msa1207950:CDS 45.0%
AGGATAAGGACTCCAAGTTC+AGG + chr8_1:65797377-65797396 Msa1207950:CDS 45.0%
CTTACTACTTCCAGACTGGT+GGG - chr8_1:65797465-65797484 None:intergenic 45.0%
ACTTACTACTTCCAGACTGG+TGG - chr8_1:65797466-65797485 None:intergenic 45.0%
CCCGTGGTAGTACTAGAAAA+AGG + chr8_1:65797533-65797552 Msa1207950:intron 45.0%
GAGTAGAAAAATGTCTGCCG+AGG - chr8_1:65797738-65797757 None:intergenic 45.0%
TGTCTTTGTGTTTGCTTCCG+TGG + chr8_1:65797819-65797838 Msa1207950:intron 45.0%
ATCAACAACTGCCAGCACTT+TGG + chr8_1:65798142-65798161 Msa1207950:CDS 45.0%
TCTCTAAGCAACCAAAGTGC+TGG - chr8_1:65798156-65798175 None:intergenic 45.0%
!! TTTGGTGTCATGATCAGTCC+TGG + chr8_1:65798248-65798267 Msa1207950:three_prime_UTR 45.0%
TGTTGATGTTGCGTTGGGTT+TGG + chr8_1:65798470-65798489 Msa1207950:three_prime_UTR 45.0%
!!! TTTTTTTTAATTTGAATTAT+AGG + chr8_1:65796540-65796559 Msa1207950:intron 5.0%
!!! TTTTTTTTAATTTGAATTAT+AGG + chr8_1:65796540-65796559 Msa1207950:intron 5.0%
AGCTGCGAGCTTCGTGATTA+GGG - chr8_1:65796199-65796218 None:intergenic 50.0%
AAGCTGCGAGCTTCGTGATT+AGG - chr8_1:65796200-65796219 None:intergenic 50.0%
CTTAGGATCCTCAAGGCTCA+CGG + chr8_1:65796679-65796698 Msa1207950:CDS 50.0%
AGAACATACCGTGAGCCTTG+AGG - chr8_1:65796690-65796709 None:intergenic 50.0%
ACTTGCACCGGAAATTCCTG+AGG + chr8_1:65797294-65797313 Msa1207950:CDS 50.0%
!! TGTGTTGCTTTGTTTGCCCG+TGG + chr8_1:65797517-65797536 Msa1207950:intron 50.0%
!! TGCTGTTCTGCAGTCGCTTT+TGG + chr8_1:65798198-65798217 Msa1207950:three_prime_UTR 50.0%
GTTGCGTTGGGTTTGGACTT+TGG + chr8_1:65798477-65798496 Msa1207950:three_prime_UTR 50.0%
AGCTGCGAGCTTCGTGATTA+GGG - chr8_1:65796199-65796218 None:intergenic 50.0%
AAGCTGCGAGCTTCGTGATT+AGG - chr8_1:65796200-65796219 None:intergenic 50.0%
CTTAGGATCCTCAAGGCTCA+CGG + chr8_1:65796679-65796698 Msa1207950:CDS 50.0%
AGAACATACCGTGAGCCTTG+AGG - chr8_1:65796690-65796709 None:intergenic 50.0%
ACTTGCACCGGAAATTCCTG+AGG + chr8_1:65797294-65797313 Msa1207950:CDS 50.0%
!! TGTGTTGCTTTGTTTGCCCG+TGG + chr8_1:65797517-65797536 Msa1207950:intron 50.0%
!! TGCTGTTCTGCAGTCGCTTT+TGG + chr8_1:65798198-65798217 Msa1207950:three_prime_UTR 50.0%
GTTGCGTTGGGTTTGGACTT+TGG + chr8_1:65798477-65798496 Msa1207950:three_prime_UTR 50.0%
CCTGGTGCAGACCTCTTGTA+TGG - chr8_1:65796410-65796429 None:intergenic 55.0%
CCATACAAGAGGTCTGCACC+AGG + chr8_1:65796407-65796426 Msa1207950:CDS 55.0%
ACAAGAGGTCTGCACCAGGA+TGG + chr8_1:65796411-65796430 Msa1207950:CDS 55.0%
GTAGAGGTCTTGAGCCATCC+TGG - chr8_1:65796428-65796447 None:intergenic 55.0%
CGTGAGCCTTGAGGATCCTA+AGG - chr8_1:65796681-65796700 None:intergenic 55.0%
TCTAGCATCAGGCAGGCATG+TGG - chr8_1:65797991-65798010 None:intergenic 55.0%
CCTGGTGCAGACCTCTTGTA+TGG - chr8_1:65796410-65796429 None:intergenic 55.0%
CCATACAAGAGGTCTGCACC+AGG + chr8_1:65796407-65796426 Msa1207950:CDS 55.0%
ACAAGAGGTCTGCACCAGGA+TGG + chr8_1:65796411-65796430 Msa1207950:CDS 55.0%
GTAGAGGTCTTGAGCCATCC+TGG - chr8_1:65796428-65796447 None:intergenic 55.0%
CGTGAGCCTTGAGGATCCTA+AGG - chr8_1:65796681-65796700 None:intergenic 55.0%
TCTAGCATCAGGCAGGCATG+TGG - chr8_1:65797991-65798010 None:intergenic 55.0%
ACCATGGGGCGTATGCACAG+TGG + chr8_1:65796232-65796251 Msa1207950:exon 60.0%
ATGGGGCGTATGCACAGTGG+CGG + chr8_1:65796235-65796254 Msa1207950:CDS 60.0%
CCAGACTGGTGGGAGCTTCT+TGG - chr8_1:65797455-65797474 None:intergenic 60.0%
CCAAGAAGCTCCCACCAGTC+TGG + chr8_1:65797452-65797471 Msa1207950:CDS 60.0%
ACCATGGGGCGTATGCACAG+TGG + chr8_1:65796232-65796251 Msa1207950:exon 60.0%
ATGGGGCGTATGCACAGTGG+CGG + chr8_1:65796235-65796254 Msa1207950:CDS 60.0%
CCAGACTGGTGGGAGCTTCT+TGG - chr8_1:65797455-65797474 None:intergenic 60.0%
CCAAGAAGCTCCCACCAGTC+TGG + chr8_1:65797452-65797471 Msa1207950:CDS 60.0%
GCCACTGTGCATACGCCCCA+TGG - chr8_1:65796236-65796255 None:intergenic 65.0%
TGGGGCGTATGCACAGTGGC+GGG + chr8_1:65796236-65796255 Msa1207950:CDS 65.0%
GCCACTGTGCATACGCCCCA+TGG - chr8_1:65796236-65796255 None:intergenic 65.0%
TGGGGCGTATGCACAGTGGC+GGG + chr8_1:65796236-65796255 Msa1207950:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 65796153 65798544 65796153 ID=Msa1207950;Name=Msa1207950
chr8_1 mRNA 65796153 65798544 65796153 ID=Msa1207950-mRNA-1;Parent=Msa1207950;Name=Msa1207950-mRNA-1;_AED=0.00;_eAED=0.00;_QI=82|1|1|1|0.75|0.8|5|372|151
chr8_1 exon 65796153 65796257 65796153 ID=Msa1207950-mRNA-1:exon:21614;Parent=Msa1207950-mRNA-1
chr8_1 exon 65796382 65796454 65796382 ID=Msa1207950-mRNA-1:exon:21615;Parent=Msa1207950-mRNA-1
chr8_1 exon 65796562 65796700 65796562 ID=Msa1207950-mRNA-1:exon:21616;Parent=Msa1207950-mRNA-1
chr8_1 exon 65797293 65797479 65797293 ID=Msa1207950-mRNA-1:exon:21617;Parent=Msa1207950-mRNA-1
chr8_1 exon 65798139 65798544 65798139 ID=Msa1207950-mRNA-1:exon:21618;Parent=Msa1207950-mRNA-1
chr8_1 five_prime_UTR 65796153 65796234 65796153 ID=Msa1207950-mRNA-1:five_prime_utr;Parent=Msa1207950-mRNA-1
chr8_1 CDS 65796235 65796257 65796235 ID=Msa1207950-mRNA-1:cds;Parent=Msa1207950-mRNA-1
chr8_1 CDS 65796382 65796454 65796382 ID=Msa1207950-mRNA-1:cds;Parent=Msa1207950-mRNA-1
chr8_1 CDS 65796562 65796700 65796562 ID=Msa1207950-mRNA-1:cds;Parent=Msa1207950-mRNA-1
chr8_1 CDS 65797293 65797479 65797293 ID=Msa1207950-mRNA-1:cds;Parent=Msa1207950-mRNA-1
chr8_1 CDS 65798139 65798172 65798139 ID=Msa1207950-mRNA-1:cds;Parent=Msa1207950-mRNA-1
chr8_1 three_prime_UTR 65798173 65798544 65798173 ID=Msa1207950-mRNA-1:three_prime_utr;Parent=Msa1207950-mRNA-1
Gene Sequence

>Msa1207950

ATGGGGCGTATGCACAGTGGCGGTAAGGGTATTTCATCTTCTGCTTTACCATACAAGAGGTCTGCACCAGGATGGCTCAAGACCTCTACACAAGATGTGGAAGAAACTATTTGCAAGTTTGCAAAGAAGGGTTTGACTCCATCTCAGATCGGTGTTATTCTTAGGGATTCTCATGGAATTGCTCAAGTCAAGTTTATAACTGGCAGCAAAATCCTTAGGATCCTCAAGGCTCACGGACTTGCACCGGAAATTCCTGAGGATCTGTACCATTTGATCAAGAAGGCAGTTTCAATTAGGAAGCATTTGGAGAGGAACAGAAAGGATAAGGACTCCAAGTTCAGGTTGATTCTTGTGGAGAGCAGAATTCACCGTCTTGCTCGCTATTACAAGAAGACCAAGAAGCTCCCACCAGTCTGGAAGTACGAATCAACAACTGCCAGCACTTTGGTTGCTTAG
Protein sequence

>Msa1207950

MGRMHSGGKGISSSALPYKRSAPGWLKTSTQDVEETICKFAKKGLTPSQIGVILRDSHGIAQVKFITGSKILRILKAHGLAPEIPEDLYHLIKKAVSIRKHLERNRKDKDSKFRLILVESRIHRLARYYKKTKKLPPVWKYESTTASTLVA*