AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1258110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1258110 MtrunA17_Chr4g0056921 90.278 72 7 0 1 72 1 72 2.50e-42 134
Msa1258110 MtrunA17_Chr4g0011261 86.567 67 9 0 1 67 1 67 1.12e-37 122
Msa1258110 MtrunA17_Chr5g0398801 77.778 72 16 0 1 72 1 72 2.78e-36 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1258110 AT4G00100.1 77.778 72 16 0 1 72 1 72 5.79e-37 121
Msa1258110 AT3G60770.1 77.778 72 16 0 1 72 1 72 1.14e-36 120

Find 39 sgRNAs with CRISPR-Local

Find 283 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAGATCGGTGTTATTCTT+AGG 0.199752 8_2:+59263726 Msa1258110:CDS
TGCTGTTCTGCAGTCGCTTT+TGG 0.209015 8_2:+59265315 Msa1258110:three_prime_UTR
ATCCTTCAGATGCAGTAAAA+TGG 0.243022 8_2:-59265528 None:intergenic
AAGCTGCGAGCTTCGTGATT+AGG 0.287127 8_2:-59263315 None:intergenic
CATCTGAAGGATACATTGTT+TGG 0.295241 8_2:+59265539 Msa1258110:three_prime_UTR
TTAACTAGTTCAAGTCTTCC+AGG 0.332262 8_2:-59265383 None:intergenic
AGCTGCGAGCTTCGTGATTA+GGG 0.339227 8_2:-59263314 None:intergenic
TGTTGATGTTGCGTTGGGTT+TGG 0.400753 8_2:+59265579 Msa1258110:three_prime_UTR
TCAGATCGGTGTTATTCTTA+GGG 0.403625 8_2:+59263727 Msa1258110:CDS
TATTTGCAAGTTTGCAAAGA+AGG 0.423456 8_2:+59263691 Msa1258110:CDS
TCCTTCAGATGCAGTAAAAT+GGG 0.425309 8_2:-59265527 None:intergenic
AGTGAACCAGAACTAGGGTT+TGG 0.452696 8_2:+59263257 None:intergenic
GACTAGAGGTGTAAGTGGTT+CGG 0.458913 8_2:+59265601 Msa1258110:three_prime_UTR
TTTGGTGTCATGATCAGTCC+TGG 0.466230 8_2:+59265365 Msa1258110:three_prime_UTR
GTTTGGACTTTCGGTTTCGT+TGG 0.486230 8_2:-59265630 None:intergenic
CCTGGTGCAGACCTCTTGTA+TGG 0.493479 8_2:-59263525 None:intergenic
GCTTAAAGCTTCAGCAACCA+TGG 0.503394 8_2:+59263334 Msa1258110:exon
GTTATTCTTAGGGATTCTCA+TGG 0.504564 8_2:+59263737 Msa1258110:CDS
ATTTGCAAGTTTGCAAAGAA+GGG 0.505113 8_2:+59263692 Msa1258110:CDS
ATCAACAACTGCCAGCACTT+TGG 0.526669 8_2:+59265259 Msa1258110:CDS
GTTTGGACTAGAGGTGTAAG+TGG 0.527057 8_2:+59265596 Msa1258110:three_prime_UTR
CTTAAAGCTTCAGCAACCAT+GGG 0.528681 8_2:+59263335 Msa1258110:exon
GTAGAGGTCTTGAGCCATCC+TGG 0.536330 8_2:-59263543 None:intergenic
TTGCGTTGGGTTTGGACTAG+AGG 0.537622 8_2:+59265587 Msa1258110:three_prime_UTR
AAACATGTTGATGTTGCGTT+GGG 0.538051 8_2:+59265574 Msa1258110:three_prime_UTR
GCCACTGTGCATACGCCCCA+TGG 0.542099 8_2:-59263351 None:intergenic
AGCATAACAAGTCAAAATCA+AGG 0.544124 8_2:-59265296 None:intergenic
AGAATAACACCGATCTGAGA+TGG 0.551609 8_2:-59263722 None:intergenic
TCTCTAAGCAACCAAAGTGC+TGG 0.564231 8_2:-59265270 None:intergenic
TGGGGCGTATGCACAGTGGC+GGG 0.570294 8_2:+59263354 Msa1258110:CDS
AAAACATGTTGATGTTGCGT+TGG 0.577507 8_2:+59265573 Msa1258110:three_prime_UTR
CCATACAAGAGGTCTGCACC+AGG 0.584958 8_2:+59263525 Msa1258110:CDS
CTTCTGCTTTACCATACAAG+AGG 0.597371 8_2:+59263514 Msa1258110:CDS
ATGGGGCGTATGCACAGTGG+CGG 0.600106 8_2:+59263353 Msa1258110:CDS
ACAAGAGGTCTGCACCAGGA+TGG 0.633754 8_2:+59263529 Msa1258110:CDS
CTGTTGTCATTTGAAAGATG+TGG 0.649779 8_2:+59265452 Msa1258110:three_prime_UTR
GGTTTGACTCCATCTCAGAT+CGG 0.677869 8_2:+59263713 Msa1258110:CDS
TTAAAGCTTCAGCAACCATG+GGG 0.724366 8_2:+59263336 Msa1258110:exon
ACCATGGGGCGTATGCACAG+TGG 0.730613 8_2:+59263350 Msa1258110:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTATATATCTAATAATATAA+TGG + chr8_2:59264980-59264999 Msa1258110:intron 10.0%
!! CTATATATCTAATAATATAA+TGG + chr8_2:59264980-59264999 Msa1258110:intron 10.0%
!!! TTTTGAATTGTTTCAAATTT+TGG + chr8_2:59263471-59263490 Msa1258110:intron 15.0%
!! CAATAACAAAAAATGATAAT+GGG - chr8_2:59263591-59263610 None:intergenic 15.0%
!! ACAATAACAAAAAATGATAA+TGG - chr8_2:59263592-59263611 None:intergenic 15.0%
!!! TTTTTAATTTGAATTATAGG+TGG + chr8_2:59263661-59263680 Msa1258110:intron 15.0%
!! TAACATAAAAGAAGAAAAAA+TGG - chr8_2:59264686-59264705 None:intergenic 15.0%
!!! TTTTGAATTGTTTCAAATTT+TGG + chr8_2:59263471-59263490 Msa1258110:intron 15.0%
!! CAATAACAAAAAATGATAAT+GGG - chr8_2:59263591-59263610 None:intergenic 15.0%
!! ACAATAACAAAAAATGATAA+TGG - chr8_2:59263592-59263611 None:intergenic 15.0%
!!! TTTTTAATTTGAATTATAGG+TGG + chr8_2:59263661-59263680 Msa1258110:intron 15.0%
!! TAACATAAAAGAAGAAAAAA+TGG - chr8_2:59264686-59264705 None:intergenic 15.0%
!! ACAATTCAAAACAAAAACAT+TGG - chr8_2:59263462-59263481 None:intergenic 20.0%
!!! ATTTGTGTCATTATAGATTA+TGG + chr8_2:59263852-59263871 Msa1258110:intron 20.0%
!!! TTTGTGTCATTATAGATTAT+GGG + chr8_2:59263853-59263872 Msa1258110:intron 20.0%
!!! TTTTCAGTTTATTTGCATAT+GGG + chr8_2:59263978-59263997 Msa1258110:intron 20.0%
!! TTATGTGAATTTAGATAAAC+AGG + chr8_2:59264199-59264218 Msa1258110:intron 20.0%
!! ATATTATAAGCACTTATGAT+AGG + chr8_2:59264304-59264323 Msa1258110:intron 20.0%
!!! TAGTAAATGAGATTTCTTTT+GGG + chr8_2:59264377-59264396 Msa1258110:intron 20.0%
!! AGATTATTTGATCTATTTGA+AGG + chr8_2:59264750-59264769 Msa1258110:intron 20.0%
!! ACATTTAATACAATAGCATA+GGG - chr8_2:59264787-59264806 None:intergenic 20.0%
!! TTAAATGTCAATCTATTTGA+AGG + chr8_2:59264799-59264818 Msa1258110:intron 20.0%
!!! TATATAGCTTTTGTTGAAAA+TGG - chr8_2:59264967-59264986 None:intergenic 20.0%
!!! TATAATGTTATTGCTTTTGT+CGG + chr8_2:59265156-59265175 Msa1258110:intron 20.0%
!! CCAAAAAAAATACAAGATAT+TGG - chr8_2:59265189-59265208 None:intergenic 20.0%
!!! CCAATATCTTGTATTTTTTT+TGG + chr8_2:59265186-59265205 Msa1258110:intron 20.0%
!!! ATTTTAAGTTATATACATGC+TGG + chr8_2:59265497-59265516 Msa1258110:three_prime_UTR 20.0%
!! ACAATTCAAAACAAAAACAT+TGG - chr8_2:59263462-59263481 None:intergenic 20.0%
!!! ATTTGTGTCATTATAGATTA+TGG + chr8_2:59263852-59263871 Msa1258110:intron 20.0%
!!! TTTGTGTCATTATAGATTAT+GGG + chr8_2:59263853-59263872 Msa1258110:intron 20.0%
!!! TTTTCAGTTTATTTGCATAT+GGG + chr8_2:59263978-59263997 Msa1258110:intron 20.0%
!! TTATGTGAATTTAGATAAAC+AGG + chr8_2:59264199-59264218 Msa1258110:intron 20.0%
!! ATATTATAAGCACTTATGAT+AGG + chr8_2:59264304-59264323 Msa1258110:intron 20.0%
!!! TAGTAAATGAGATTTCTTTT+GGG + chr8_2:59264377-59264396 Msa1258110:intron 20.0%
!! AGATTATTTGATCTATTTGA+AGG + chr8_2:59264750-59264769 Msa1258110:intron 20.0%
!! ACATTTAATACAATAGCATA+GGG - chr8_2:59264787-59264806 None:intergenic 20.0%
!! TTAAATGTCAATCTATTTGA+AGG + chr8_2:59264799-59264818 Msa1258110:intron 20.0%
!!! TATATAGCTTTTGTTGAAAA+TGG - chr8_2:59264967-59264986 None:intergenic 20.0%
!!! TATAATGTTATTGCTTTTGT+CGG + chr8_2:59265156-59265175 Msa1258110:intron 20.0%
!! CCAAAAAAAATACAAGATAT+TGG - chr8_2:59265189-59265208 None:intergenic 20.0%
!!! CCAATATCTTGTATTTTTTT+TGG + chr8_2:59265186-59265205 Msa1258110:intron 20.0%
!!! ATTTTAAGTTATATACATGC+TGG + chr8_2:59265497-59265516 Msa1258110:three_prime_UTR 20.0%
! ATTTCACAAAAATCATCGTT+TGG + chr8_2:59263280-59263299 Msa1258110:five_prime_UTR 25.0%
! TTTCACAAAAATCATCGTTT+GGG + chr8_2:59263281-59263300 Msa1258110:five_prime_UTR 25.0%
!!! TTCAAATTTTGGTTACAGTA+AGG + chr8_2:59263482-59263501 Msa1258110:intron 25.0%
!!! TCAAATTTTGGTTACAGTAA+GGG + chr8_2:59263483-59263502 Msa1258110:intron 25.0%
! AAAAATTACATCTTGTGTAG+AGG - chr8_2:59263562-59263581 None:intergenic 25.0%
! CTACCAAATAAAGTTCTTAT+GGG - chr8_2:59263893-59263912 None:intergenic 25.0%
! ACTACCAAATAAAGTTCTTA+TGG - chr8_2:59263894-59263913 None:intergenic 25.0%
!! GTTTTCAGTTTATTTGCATA+TGG + chr8_2:59263977-59263996 Msa1258110:intron 25.0%
! CTTATGAAAACAGGTTATAT+TGG + chr8_2:59264227-59264246 Msa1258110:intron 25.0%
!! GTAGTAAATGAGATTTCTTT+TGG + chr8_2:59264376-59264395 Msa1258110:intron 25.0%
!! AGTAAATGAGATTTCTTTTG+GGG + chr8_2:59264378-59264397 Msa1258110:intron 25.0%
!! TAGTACTAGAAAAAGGATTT+TGG + chr8_2:59264658-59264677 Msa1258110:intron 25.0%
! ATTTAATACAATAGCATAGG+GGG - chr8_2:59264785-59264804 None:intergenic 25.0%
! CATTTAATACAATAGCATAG+GGG - chr8_2:59264786-59264805 None:intergenic 25.0%
! GACATTTAATACAATAGCAT+AGG - chr8_2:59264788-59264807 None:intergenic 25.0%
!! CATTTTTCTACTCTTTCTAT+TGG + chr8_2:59264863-59264882 Msa1258110:intron 25.0%
! GTTGTATAAAACAAAGTTTC+AGG + chr8_2:59264897-59264916 Msa1258110:intron 25.0%
! TCATGTTACATTTGATACTT+TGG + chr8_2:59265020-59265039 Msa1258110:intron 25.0%
! GTTATGTTGAAATTCTATTC+TGG + chr8_2:59265048-59265067 Msa1258110:intron 25.0%
! GACAAAAGCAATAACATTAT+AGG - chr8_2:59265157-59265176 None:intergenic 25.0%
! AGTTCTACAACTGATAAAAA+AGG - chr8_2:59265430-59265449 None:intergenic 25.0%
!!! ATACATTGTTTGGTTTTTGA+AGG + chr8_2:59265549-59265568 Msa1258110:three_prime_UTR 25.0%
! ATTTCACAAAAATCATCGTT+TGG + chr8_2:59263280-59263299 Msa1258110:five_prime_UTR 25.0%
! TTTCACAAAAATCATCGTTT+GGG + chr8_2:59263281-59263300 Msa1258110:five_prime_UTR 25.0%
!!! TTCAAATTTTGGTTACAGTA+AGG + chr8_2:59263482-59263501 Msa1258110:intron 25.0%
!!! TCAAATTTTGGTTACAGTAA+GGG + chr8_2:59263483-59263502 Msa1258110:intron 25.0%
! AAAAATTACATCTTGTGTAG+AGG - chr8_2:59263562-59263581 None:intergenic 25.0%
! CTACCAAATAAAGTTCTTAT+GGG - chr8_2:59263893-59263912 None:intergenic 25.0%
! ACTACCAAATAAAGTTCTTA+TGG - chr8_2:59263894-59263913 None:intergenic 25.0%
!! GTTTTCAGTTTATTTGCATA+TGG + chr8_2:59263977-59263996 Msa1258110:intron 25.0%
! CTTATGAAAACAGGTTATAT+TGG + chr8_2:59264227-59264246 Msa1258110:intron 25.0%
!! GTAGTAAATGAGATTTCTTT+TGG + chr8_2:59264376-59264395 Msa1258110:intron 25.0%
!! AGTAAATGAGATTTCTTTTG+GGG + chr8_2:59264378-59264397 Msa1258110:intron 25.0%
!! TAGTACTAGAAAAAGGATTT+TGG + chr8_2:59264658-59264677 Msa1258110:intron 25.0%
! ATTTAATACAATAGCATAGG+GGG - chr8_2:59264785-59264804 None:intergenic 25.0%
! CATTTAATACAATAGCATAG+GGG - chr8_2:59264786-59264805 None:intergenic 25.0%
! GACATTTAATACAATAGCAT+AGG - chr8_2:59264788-59264807 None:intergenic 25.0%
!! CATTTTTCTACTCTTTCTAT+TGG + chr8_2:59264863-59264882 Msa1258110:intron 25.0%
! GTTGTATAAAACAAAGTTTC+AGG + chr8_2:59264897-59264916 Msa1258110:intron 25.0%
! TCATGTTACATTTGATACTT+TGG + chr8_2:59265020-59265039 Msa1258110:intron 25.0%
! GTTATGTTGAAATTCTATTC+TGG + chr8_2:59265048-59265067 Msa1258110:intron 25.0%
! GACAAAAGCAATAACATTAT+AGG - chr8_2:59265157-59265176 None:intergenic 25.0%
! AGTTCTACAACTGATAAAAA+AGG - chr8_2:59265430-59265449 None:intergenic 25.0%
!!! ATACATTGTTTGGTTTTTGA+AGG + chr8_2:59265549-59265568 Msa1258110:three_prime_UTR 25.0%
TATTTGCAAGTTTGCAAAGA+AGG + chr8_2:59263691-59263710 Msa1258110:CDS 30.0%
ATTTGCAAGTTTGCAAAGAA+GGG + chr8_2:59263692-59263711 Msa1258110:CDS 30.0%
TGACCCATAAGAACTTTATT+TGG + chr8_2:59263887-59263906 Msa1258110:intron 30.0%
AAGCACTTGTAAAACTGATT+AGG + chr8_2:59264092-59264111 Msa1258110:intron 30.0%
AAGCACTTGTGAAATTGATT+AGG + chr8_2:59264148-59264167 Msa1258110:intron 30.0%
!!! TTTCTTTTGGGGTTGATTTT+AGG + chr8_2:59264389-59264408 Msa1258110:intron 30.0%
!! AGTTTCAATTAGGAAGCATT+TGG + chr8_2:59264460-59264479 Msa1258110:intron 30.0%
! AAATTTTACACTTTGCACCT+CGG + chr8_2:59264836-59264855 Msa1258110:intron 30.0%
TTGTTGAAAATGGATATCCA+CGG - chr8_2:59264957-59264976 None:intergenic 30.0%
TAACCTAAAATTCTAGCATC+AGG - chr8_2:59265120-59265139 None:intergenic 30.0%
AGAGACAAGTCATTAATATC+AGG - chr8_2:59265229-59265248 None:intergenic 30.0%
AGCATAACAAGTCAAAATCA+AGG - chr8_2:59265299-59265318 None:intergenic 30.0%
!!! TTGCTCGAATGTAGTTTTTT+TGG + chr8_2:59265347-59265366 Msa1258110:three_prime_UTR 30.0%
TATTTGCAAGTTTGCAAAGA+AGG + chr8_2:59263691-59263710 Msa1258110:CDS 30.0%
ATTTGCAAGTTTGCAAAGAA+GGG + chr8_2:59263692-59263711 Msa1258110:CDS 30.0%
TGACCCATAAGAACTTTATT+TGG + chr8_2:59263887-59263906 Msa1258110:intron 30.0%
AAGCACTTGTAAAACTGATT+AGG + chr8_2:59264092-59264111 Msa1258110:intron 30.0%
AAGCACTTGTGAAATTGATT+AGG + chr8_2:59264148-59264167 Msa1258110:intron 30.0%
!!! TTTCTTTTGGGGTTGATTTT+AGG + chr8_2:59264389-59264408 Msa1258110:intron 30.0%
!! AGTTTCAATTAGGAAGCATT+TGG + chr8_2:59264460-59264479 Msa1258110:intron 30.0%
! AAATTTTACACTTTGCACCT+CGG + chr8_2:59264836-59264855 Msa1258110:intron 30.0%
TTGTTGAAAATGGATATCCA+CGG - chr8_2:59264957-59264976 None:intergenic 30.0%
TAACCTAAAATTCTAGCATC+AGG - chr8_2:59265120-59265139 None:intergenic 30.0%
AGAGACAAGTCATTAATATC+AGG - chr8_2:59265229-59265248 None:intergenic 30.0%
AGCATAACAAGTCAAAATCA+AGG - chr8_2:59265299-59265318 None:intergenic 30.0%
!!! TTGCTCGAATGTAGTTTTTT+TGG + chr8_2:59265347-59265366 Msa1258110:three_prime_UTR 30.0%
TTGCGATTGAGATTGAGAAT+GGG - chr8_2:59263393-59263412 None:intergenic 35.0%
ATTGCGATTGAGATTGAGAA+TGG - chr8_2:59263394-59263413 None:intergenic 35.0%
!! TCAGATCGGTGTTATTCTTA+GGG + chr8_2:59263727-59263746 Msa1258110:CDS 35.0%
GTTATTCTTAGGGATTCTCA+TGG + chr8_2:59263737-59263756 Msa1258110:CDS 35.0%
ATCAATTTCACAAGTGCTTG+TGG - chr8_2:59264147-59264166 None:intergenic 35.0%
TCATAGAAACAGTGTATGAC+AGG + chr8_2:59264176-59264195 Msa1258110:intron 35.0%
TCTGTACCATTTGATCAAGA+AGG + chr8_2:59264436-59264455 Msa1258110:intron 35.0%
AAACTGCCTTCTTGATCAAA+TGG - chr8_2:59264445-59264464 None:intergenic 35.0%
TCAAGAAGGCAGTTTCAATT+AGG + chr8_2:59264450-59264469 Msa1258110:intron 35.0%
!! TCCTTTTTCTAGTACTACCA+CGG - chr8_2:59264655-59264674 None:intergenic 35.0%
TCACTTCACACATCAAAACA+TGG - chr8_2:59264730-59264749 None:intergenic 35.0%
!! TATTCTGGTTCCTTGTTTGA+TGG + chr8_2:59265063-59265082 Msa1258110:intron 35.0%
!! ATTCTGGTTCCTTGTTTGAT+GGG + chr8_2:59265064-59265083 Msa1258110:intron 35.0%
ATAAAGAAGCCCATCAAACA+AGG - chr8_2:59265076-59265095 None:intergenic 35.0%
!! ATGTTATTGCTTTTGTCGGT+TGG + chr8_2:59265160-59265179 Msa1258110:intron 35.0%
TTAACTAGTTCAAGTCTTCC+AGG - chr8_2:59265386-59265405 None:intergenic 35.0%
! CTGTTGTCATTTGAAAGATG+TGG + chr8_2:59265452-59265471 Msa1258110:three_prime_UTR 35.0%
TCCTTCAGATGCAGTAAAAT+GGG - chr8_2:59265530-59265549 None:intergenic 35.0%
ATCCTTCAGATGCAGTAAAA+TGG - chr8_2:59265531-59265550 None:intergenic 35.0%
CATCTGAAGGATACATTGTT+TGG + chr8_2:59265539-59265558 Msa1258110:three_prime_UTR 35.0%
AAAACATGTTGATGTTGCGT+TGG + chr8_2:59265573-59265592 Msa1258110:three_prime_UTR 35.0%
AAACATGTTGATGTTGCGTT+GGG + chr8_2:59265574-59265593 Msa1258110:three_prime_UTR 35.0%
TTGCGATTGAGATTGAGAAT+GGG - chr8_2:59263393-59263412 None:intergenic 35.0%
ATTGCGATTGAGATTGAGAA+TGG - chr8_2:59263394-59263413 None:intergenic 35.0%
!! TCAGATCGGTGTTATTCTTA+GGG + chr8_2:59263727-59263746 Msa1258110:CDS 35.0%
GTTATTCTTAGGGATTCTCA+TGG + chr8_2:59263737-59263756 Msa1258110:CDS 35.0%
ATCAATTTCACAAGTGCTTG+TGG - chr8_2:59264147-59264166 None:intergenic 35.0%
TCATAGAAACAGTGTATGAC+AGG + chr8_2:59264176-59264195 Msa1258110:intron 35.0%
TCTGTACCATTTGATCAAGA+AGG + chr8_2:59264436-59264455 Msa1258110:intron 35.0%
AAACTGCCTTCTTGATCAAA+TGG - chr8_2:59264445-59264464 None:intergenic 35.0%
TCAAGAAGGCAGTTTCAATT+AGG + chr8_2:59264450-59264469 Msa1258110:intron 35.0%
!! TCCTTTTTCTAGTACTACCA+CGG - chr8_2:59264655-59264674 None:intergenic 35.0%
TCACTTCACACATCAAAACA+TGG - chr8_2:59264730-59264749 None:intergenic 35.0%
!! TATTCTGGTTCCTTGTTTGA+TGG + chr8_2:59265063-59265082 Msa1258110:intron 35.0%
!! ATTCTGGTTCCTTGTTTGAT+GGG + chr8_2:59265064-59265083 Msa1258110:intron 35.0%
ATAAAGAAGCCCATCAAACA+AGG - chr8_2:59265076-59265095 None:intergenic 35.0%
!! ATGTTATTGCTTTTGTCGGT+TGG + chr8_2:59265160-59265179 Msa1258110:intron 35.0%
TTAACTAGTTCAAGTCTTCC+AGG - chr8_2:59265386-59265405 None:intergenic 35.0%
! CTGTTGTCATTTGAAAGATG+TGG + chr8_2:59265452-59265471 Msa1258110:three_prime_UTR 35.0%
TCCTTCAGATGCAGTAAAAT+GGG - chr8_2:59265530-59265549 None:intergenic 35.0%
ATCCTTCAGATGCAGTAAAA+TGG - chr8_2:59265531-59265550 None:intergenic 35.0%
CATCTGAAGGATACATTGTT+TGG + chr8_2:59265539-59265558 Msa1258110:three_prime_UTR 35.0%
AAAACATGTTGATGTTGCGT+TGG + chr8_2:59265573-59265592 Msa1258110:three_prime_UTR 35.0%
AAACATGTTGATGTTGCGTT+GGG + chr8_2:59265574-59265593 Msa1258110:three_prime_UTR 35.0%
CTTAAAGCTTCAGCAACCAT+GGG + chr8_2:59263335-59263354 Msa1258110:exon 40.0%
TTAAAGCTTCAGCAACCATG+GGG + chr8_2:59263336-59263355 Msa1258110:exon 40.0%
TTGAGATTGAGAATGGGGAA+TGG - chr8_2:59263387-59263406 None:intergenic 40.0%
TGCGATTGAGATTGAGAATG+GGG - chr8_2:59263392-59263411 None:intergenic 40.0%
CTTCTGCTTTACCATACAAG+AGG + chr8_2:59263514-59263533 Msa1258110:CDS 40.0%
AGAATAACACCGATCTGAGA+TGG - chr8_2:59263725-59263744 None:intergenic 40.0%
!! CTCAGATCGGTGTTATTCTT+AGG + chr8_2:59263726-59263745 Msa1258110:CDS 40.0%
GCTCAAGTCAAGTTTATCAC+TGG + chr8_2:59263764-59263783 Msa1258110:intron 40.0%
CAAAATCCTTAGGATCCTCA+AGG + chr8_2:59263790-59263809 Msa1258110:intron 40.0%
CAGGCATAGCTTATGAAAAC+AGG + chr8_2:59264218-59264237 Msa1258110:intron 40.0%
!!! GTTGATTTTAGGACTTGCAC+CGG + chr8_2:59264400-59264419 Msa1258110:intron 40.0%
TACAGATCCTCAGGAATTTC+CGG - chr8_2:59264422-59264441 None:intergenic 40.0%
ATCAAATGGTACAGATCCTC+AGG - chr8_2:59264431-59264450 None:intergenic 40.0%
!! CAATTAGGAAGCATTTGGAG+AGG + chr8_2:59264465-59264484 Msa1258110:intron 40.0%
CACAAGAATCAACCTGAACT+TGG - chr8_2:59264510-59264529 None:intergenic 40.0%
!! CAAGTTCAGGTTGATTCTTG+TGG + chr8_2:59264508-59264527 Msa1258110:intron 40.0%
TCTTGTAATAGCGAGCAAGA+CGG - chr8_2:59264547-59264566 None:intergenic 40.0%
AGTACTTACTACTTCCAGAC+TGG - chr8_2:59264587-59264606 None:intergenic 40.0%
!! CCTTTTTCTAGTACTACCAC+GGG - chr8_2:59264654-59264673 None:intergenic 40.0%
CTAAAATTCTAGCATCAGGC+AGG - chr8_2:59265116-59265135 None:intergenic 40.0%
! CTGCCTGATGCTAGAATTTT+AGG + chr8_2:59265114-59265133 Msa1258110:intron 40.0%
!! ATGTGGCTGACTTCAGTTTT+TGG + chr8_2:59265469-59265488 Msa1258110:three_prime_UTR 40.0%
! ACCCATTTTACTGCATCTGA+AGG + chr8_2:59265526-59265545 Msa1258110:three_prime_UTR 40.0%
ACAAAACCAACGAAACCGAA+AGG + chr8_2:59265624-59265643 Msa1258110:three_prime_UTR 40.0%
CTTAAAGCTTCAGCAACCAT+GGG + chr8_2:59263335-59263354 Msa1258110:exon 40.0%
TTAAAGCTTCAGCAACCATG+GGG + chr8_2:59263336-59263355 Msa1258110:exon 40.0%
TTGAGATTGAGAATGGGGAA+TGG - chr8_2:59263387-59263406 None:intergenic 40.0%
TGCGATTGAGATTGAGAATG+GGG - chr8_2:59263392-59263411 None:intergenic 40.0%
CTTCTGCTTTACCATACAAG+AGG + chr8_2:59263514-59263533 Msa1258110:CDS 40.0%
AGAATAACACCGATCTGAGA+TGG - chr8_2:59263725-59263744 None:intergenic 40.0%
!! CTCAGATCGGTGTTATTCTT+AGG + chr8_2:59263726-59263745 Msa1258110:CDS 40.0%
GCTCAAGTCAAGTTTATCAC+TGG + chr8_2:59263764-59263783 Msa1258110:intron 40.0%
CAAAATCCTTAGGATCCTCA+AGG + chr8_2:59263790-59263809 Msa1258110:intron 40.0%
CAGGCATAGCTTATGAAAAC+AGG + chr8_2:59264218-59264237 Msa1258110:intron 40.0%
!!! GTTGATTTTAGGACTTGCAC+CGG + chr8_2:59264400-59264419 Msa1258110:intron 40.0%
TACAGATCCTCAGGAATTTC+CGG - chr8_2:59264422-59264441 None:intergenic 40.0%
ATCAAATGGTACAGATCCTC+AGG - chr8_2:59264431-59264450 None:intergenic 40.0%
!! CAATTAGGAAGCATTTGGAG+AGG + chr8_2:59264465-59264484 Msa1258110:intron 40.0%
CACAAGAATCAACCTGAACT+TGG - chr8_2:59264510-59264529 None:intergenic 40.0%
!! CAAGTTCAGGTTGATTCTTG+TGG + chr8_2:59264508-59264527 Msa1258110:intron 40.0%
TCTTGTAATAGCGAGCAAGA+CGG - chr8_2:59264547-59264566 None:intergenic 40.0%
AGTACTTACTACTTCCAGAC+TGG - chr8_2:59264587-59264606 None:intergenic 40.0%
!! CCTTTTTCTAGTACTACCAC+GGG - chr8_2:59264654-59264673 None:intergenic 40.0%
CTAAAATTCTAGCATCAGGC+AGG - chr8_2:59265116-59265135 None:intergenic 40.0%
! CTGCCTGATGCTAGAATTTT+AGG + chr8_2:59265114-59265133 Msa1258110:intron 40.0%
!! ATGTGGCTGACTTCAGTTTT+TGG + chr8_2:59265469-59265488 Msa1258110:three_prime_UTR 40.0%
! ACCCATTTTACTGCATCTGA+AGG + chr8_2:59265526-59265545 Msa1258110:three_prime_UTR 40.0%
GCTTAAAGCTTCAGCAACCA+TGG + chr8_2:59263334-59263353 Msa1258110:exon 45.0%
GGTTTGACTCCATCTCAGAT+CGG + chr8_2:59263713-59263732 Msa1258110:CDS 45.0%
TCACTGGCAGCAAAATCCTT+AGG + chr8_2:59263780-59263799 Msa1258110:intron 45.0%
TATTTGCATATGGGCGTGTG+TGG + chr8_2:59263987-59264006 Msa1258110:intron 45.0%
GCATTTGGAGAGGAACAGAA+AGG + chr8_2:59264475-59264494 Msa1258110:intron 45.0%
GGAGAGGAACAGAAAGGATA+AGG + chr8_2:59264481-59264500 Msa1258110:intron 45.0%
AGGATAAGGACTCCAAGTTC+AGG + chr8_2:59264495-59264514 Msa1258110:intron 45.0%
CTTACTACTTCCAGACTGGT+GGG - chr8_2:59264583-59264602 None:intergenic 45.0%
ACTTACTACTTCCAGACTGG+TGG - chr8_2:59264584-59264603 None:intergenic 45.0%
CCCGTGGTAGTACTAGAAAA+AGG + chr8_2:59264651-59264670 Msa1258110:intron 45.0%
GAGTAGAAAAATGTCTGCCG+AGG - chr8_2:59264856-59264875 None:intergenic 45.0%
TGTCTTTGTGTTTGCTTCCG+TGG + chr8_2:59264937-59264956 Msa1258110:intron 45.0%
ATCAACAACTGCCAGCACTT+TGG + chr8_2:59265259-59265278 Msa1258110:CDS 45.0%
TCTCTAAGCAACCAAAGTGC+TGG - chr8_2:59265273-59265292 None:intergenic 45.0%
!! TTTGGTGTCATGATCAGTCC+TGG + chr8_2:59265365-59265384 Msa1258110:three_prime_UTR 45.0%
TGTTGATGTTGCGTTGGGTT+TGG + chr8_2:59265579-59265598 Msa1258110:three_prime_UTR 45.0%
! GTTTGGACTAGAGGTGTAAG+TGG + chr8_2:59265596-59265615 Msa1258110:three_prime_UTR 45.0%
! GACTAGAGGTGTAAGTGGTT+CGG + chr8_2:59265601-59265620 Msa1258110:three_prime_UTR 45.0%
GCTTAAAGCTTCAGCAACCA+TGG + chr8_2:59263334-59263353 Msa1258110:exon 45.0%
GGTTTGACTCCATCTCAGAT+CGG + chr8_2:59263713-59263732 Msa1258110:CDS 45.0%
TCACTGGCAGCAAAATCCTT+AGG + chr8_2:59263780-59263799 Msa1258110:intron 45.0%
TATTTGCATATGGGCGTGTG+TGG + chr8_2:59263987-59264006 Msa1258110:intron 45.0%
GCATTTGGAGAGGAACAGAA+AGG + chr8_2:59264475-59264494 Msa1258110:intron 45.0%
GGAGAGGAACAGAAAGGATA+AGG + chr8_2:59264481-59264500 Msa1258110:intron 45.0%
AGGATAAGGACTCCAAGTTC+AGG + chr8_2:59264495-59264514 Msa1258110:intron 45.0%
CTTACTACTTCCAGACTGGT+GGG - chr8_2:59264583-59264602 None:intergenic 45.0%
ACTTACTACTTCCAGACTGG+TGG - chr8_2:59264584-59264603 None:intergenic 45.0%
CCCGTGGTAGTACTAGAAAA+AGG + chr8_2:59264651-59264670 Msa1258110:intron 45.0%
GAGTAGAAAAATGTCTGCCG+AGG - chr8_2:59264856-59264875 None:intergenic 45.0%
TGTCTTTGTGTTTGCTTCCG+TGG + chr8_2:59264937-59264956 Msa1258110:intron 45.0%
ATCAACAACTGCCAGCACTT+TGG + chr8_2:59265259-59265278 Msa1258110:CDS 45.0%
TCTCTAAGCAACCAAAGTGC+TGG - chr8_2:59265273-59265292 None:intergenic 45.0%
!! TTTGGTGTCATGATCAGTCC+TGG + chr8_2:59265365-59265384 Msa1258110:three_prime_UTR 45.0%
TGTTGATGTTGCGTTGGGTT+TGG + chr8_2:59265579-59265598 Msa1258110:three_prime_UTR 45.0%
! GTTTGGACTAGAGGTGTAAG+TGG + chr8_2:59265596-59265615 Msa1258110:three_prime_UTR 45.0%
! GACTAGAGGTGTAAGTGGTT+CGG + chr8_2:59265601-59265620 Msa1258110:three_prime_UTR 45.0%
!!! TTTTTTTTAATTTGAATTAT+AGG + chr8_2:59263658-59263677 Msa1258110:intron 5.0%
!!! TTTTTTTTAATTTGAATTAT+AGG + chr8_2:59263658-59263677 Msa1258110:intron 5.0%
AGCTGCGAGCTTCGTGATTA+GGG - chr8_2:59263317-59263336 None:intergenic 50.0%
AAGCTGCGAGCTTCGTGATT+AGG - chr8_2:59263318-59263337 None:intergenic 50.0%
CTTAGGATCCTCAAGGCTCA+CGG + chr8_2:59263797-59263816 Msa1258110:intron 50.0%
AGAACATACCGTGAGCCTTG+AGG - chr8_2:59263808-59263827 None:intergenic 50.0%
ACTTGCACCGGAAATTCCTG+AGG + chr8_2:59264412-59264431 Msa1258110:intron 50.0%
TGTAATAGCGAGCAAGACGG+TGG - chr8_2:59264544-59264563 None:intergenic 50.0%
!! TGTGTTGCTTTGTTTGCCCG+TGG + chr8_2:59264635-59264654 Msa1258110:intron 50.0%
!! TGCTGTTCTGCAGTCGCTTT+TGG + chr8_2:59265315-59265334 Msa1258110:three_prime_UTR 50.0%
! TTGCGTTGGGTTTGGACTAG+AGG + chr8_2:59265587-59265606 Msa1258110:three_prime_UTR 50.0%
AGCTGCGAGCTTCGTGATTA+GGG - chr8_2:59263317-59263336 None:intergenic 50.0%
AAGCTGCGAGCTTCGTGATT+AGG - chr8_2:59263318-59263337 None:intergenic 50.0%
CTTAGGATCCTCAAGGCTCA+CGG + chr8_2:59263797-59263816 Msa1258110:intron 50.0%
AGAACATACCGTGAGCCTTG+AGG - chr8_2:59263808-59263827 None:intergenic 50.0%
ACTTGCACCGGAAATTCCTG+AGG + chr8_2:59264412-59264431 Msa1258110:intron 50.0%
TGTAATAGCGAGCAAGACGG+TGG - chr8_2:59264544-59264563 None:intergenic 50.0%
!! TGTGTTGCTTTGTTTGCCCG+TGG + chr8_2:59264635-59264654 Msa1258110:intron 50.0%
!! TGCTGTTCTGCAGTCGCTTT+TGG + chr8_2:59265315-59265334 Msa1258110:three_prime_UTR 50.0%
! TTGCGTTGGGTTTGGACTAG+AGG + chr8_2:59265587-59265606 Msa1258110:three_prime_UTR 50.0%
CCTGGTGCAGACCTCTTGTA+TGG - chr8_2:59263528-59263547 None:intergenic 55.0%
CCATACAAGAGGTCTGCACC+AGG + chr8_2:59263525-59263544 Msa1258110:CDS 55.0%
ACAAGAGGTCTGCACCAGGA+TGG + chr8_2:59263529-59263548 Msa1258110:CDS 55.0%
GTAGAGGTCTTGAGCCATCC+TGG - chr8_2:59263546-59263565 None:intergenic 55.0%
CGTGAGCCTTGAGGATCCTA+AGG - chr8_2:59263799-59263818 None:intergenic 55.0%
TCTAGCATCAGGCAGGCATG+TGG - chr8_2:59265109-59265128 None:intergenic 55.0%
CCTGGTGCAGACCTCTTGTA+TGG - chr8_2:59263528-59263547 None:intergenic 55.0%
CCATACAAGAGGTCTGCACC+AGG + chr8_2:59263525-59263544 Msa1258110:CDS 55.0%
ACAAGAGGTCTGCACCAGGA+TGG + chr8_2:59263529-59263548 Msa1258110:CDS 55.0%
GTAGAGGTCTTGAGCCATCC+TGG - chr8_2:59263546-59263565 None:intergenic 55.0%
CGTGAGCCTTGAGGATCCTA+AGG - chr8_2:59263799-59263818 None:intergenic 55.0%
TCTAGCATCAGGCAGGCATG+TGG - chr8_2:59265109-59265128 None:intergenic 55.0%
ACCATGGGGCGTATGCACAG+TGG + chr8_2:59263350-59263369 Msa1258110:exon 60.0%
ATGGGGCGTATGCACAGTGG+CGG + chr8_2:59263353-59263372 Msa1258110:CDS 60.0%
CCAGACTGGTGGGAGCTTCT+TGG - chr8_2:59264573-59264592 None:intergenic 60.0%
CCAAGAAGCTCCCACCAGTC+TGG + chr8_2:59264570-59264589 Msa1258110:intron 60.0%
ACCATGGGGCGTATGCACAG+TGG + chr8_2:59263350-59263369 Msa1258110:exon 60.0%
ATGGGGCGTATGCACAGTGG+CGG + chr8_2:59263353-59263372 Msa1258110:CDS 60.0%
CCAGACTGGTGGGAGCTTCT+TGG - chr8_2:59264573-59264592 None:intergenic 60.0%
CCAAGAAGCTCCCACCAGTC+TGG + chr8_2:59264570-59264589 Msa1258110:intron 60.0%
GCCACTGTGCATACGCCCCA+TGG - chr8_2:59263354-59263373 None:intergenic 65.0%
TGGGGCGTATGCACAGTGGC+GGG + chr8_2:59263354-59263373 Msa1258110:CDS 65.0%
GCCACTGTGCATACGCCCCA+TGG - chr8_2:59263354-59263373 None:intergenic 65.0%
TGGGGCGTATGCACAGTGGC+GGG + chr8_2:59263354-59263373 Msa1258110:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 59263271 59265643 59263271 ID=Msa1258110;Name=Msa1258110
chr8_2 mRNA 59263271 59265643 59263271 ID=Msa1258110-mRNA-1;Parent=Msa1258110;Name=Msa1258110-mRNA-1;_AED=0.13;_eAED=0.13;_QI=82|0.66|0.75|1|1|1|4|354|74
chr8_2 exon 59263271 59263375 59263271 ID=Msa1258110-mRNA-1:exon:18348;Parent=Msa1258110-mRNA-1
chr8_2 exon 59263500 59263572 59263500 ID=Msa1258110-mRNA-1:exon:18349;Parent=Msa1258110-mRNA-1
chr8_2 exon 59263680 59263774 59263680 ID=Msa1258110-mRNA-1:exon:18350;Parent=Msa1258110-mRNA-1
chr8_2 exon 59265256 59265643 59265256 ID=Msa1258110-mRNA-1:exon:18351;Parent=Msa1258110-mRNA-1
chr8_2 five_prime_UTR 59263271 59263352 59263271 ID=Msa1258110-mRNA-1:five_prime_utr;Parent=Msa1258110-mRNA-1
chr8_2 CDS 59263353 59263375 59263353 ID=Msa1258110-mRNA-1:cds;Parent=Msa1258110-mRNA-1
chr8_2 CDS 59263500 59263572 59263500 ID=Msa1258110-mRNA-1:cds;Parent=Msa1258110-mRNA-1
chr8_2 CDS 59263680 59263774 59263680 ID=Msa1258110-mRNA-1:cds;Parent=Msa1258110-mRNA-1
chr8_2 CDS 59265256 59265289 59265256 ID=Msa1258110-mRNA-1:cds;Parent=Msa1258110-mRNA-1
chr8_2 three_prime_UTR 59265290 59265643 59265290 ID=Msa1258110-mRNA-1:three_prime_utr;Parent=Msa1258110-mRNA-1
Gene Sequence

>Msa1258110

ATGGGGCGTATGCACAGTGGCGGTAAGGGTATTTCATCTTCTGCTTTACCATACAAGAGGTCTGCACCAGGATGGCTCAAGACCTCTACACAAGATGTGGAAGAAACTATTTGCAAGTTTGCAAAGAAGGGTTTGACTCCATCTCAGATCGGTGTTATTCTTAGGGATTCTCATGGAATTGCTCAAGTCAACGAATCAACAACTGCCAGCACTTTGGTTGCTTAG
Protein sequence

>Msa1258110

MGRMHSGGKGISSSALPYKRSAPGWLKTSTQDVEETICKFAKKGLTPSQIGVILRDSHGIAQVNESTTASTLVA*