Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1209610 | XP_013457768.1 | 95.263 | 190 | 7 | 1 | 1 | 190 | 1 | 188 | 1.56e-122 | 353 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1209610 | sp|Q9FML4|LOB_ARATH | 68.333 | 180 | 45 | 3 | 1 | 171 | 1 | 177 | 1.76e-77 | 232 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa1209610 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1209610 | Msa1246080 | 0.946277 | 6.268399e-105 | -8.615850e-47 |
| Msa1209610 | Msa1298850 | 0.877354 | 6.722559e-69 | -8.615850e-47 |
| Msa1209610 | Msa1330510 | 0.813808 | 2.121824e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1209610 | MtrunA17_Chr4g0058651 | 95.263 | 190 | 7 | 1 | 1 | 190 | 1 | 188 | 1.43e-126 | 353 |
| Msa1209610 | MtrunA17_Chr3g0117121 | 72.881 | 177 | 46 | 2 | 3 | 178 | 2 | 177 | 8.72e-86 | 251 |
| Msa1209610 | MtrunA17_Chr7g0227511 | 62.234 | 188 | 54 | 4 | 1 | 188 | 1 | 171 | 1.17e-72 | 217 |
| Msa1209610 | MtrunA17_Chr6g0449641 | 82.301 | 113 | 18 | 1 | 1 | 113 | 1 | 111 | 2.86e-65 | 197 |
| Msa1209610 | MtrunA17_Chr6g0449651 | 79.130 | 115 | 22 | 1 | 1 | 115 | 59 | 171 | 1.95e-63 | 194 |
| Msa1209610 | MtrunA17_Chr8g0353981 | 66.917 | 133 | 40 | 1 | 1 | 133 | 1 | 129 | 9.90e-54 | 171 |
| Msa1209610 | MtrunA17_Chr5g0437571 | 64.957 | 117 | 38 | 1 | 9 | 122 | 4 | 120 | 1.45e-50 | 160 |
| Msa1209610 | MtrunA17_Chr5g0402611 | 69.091 | 110 | 34 | 0 | 5 | 114 | 2 | 111 | 3.88e-50 | 164 |
| Msa1209610 | MtrunA17_Chr3g0115431 | 64.754 | 122 | 43 | 0 | 5 | 126 | 3 | 124 | 4.69e-50 | 161 |
| Msa1209610 | MtrunA17_Chr3g0097221 | 65.179 | 112 | 39 | 0 | 6 | 117 | 2 | 113 | 3.89e-48 | 158 |
| Msa1209610 | MtrunA17_Chr2g0311601 | 63.551 | 107 | 39 | 0 | 10 | 116 | 12 | 118 | 9.54e-48 | 153 |
| Msa1209610 | MtrunA17_Chr4g0043421 | 65.385 | 104 | 36 | 0 | 10 | 113 | 14 | 117 | 2.72e-47 | 152 |
| Msa1209610 | MtrunA17_Chr3g0106101 | 64.423 | 104 | 37 | 0 | 9 | 112 | 5 | 108 | 7.58e-47 | 151 |
| Msa1209610 | MtrunA17_Chr5g0437401 | 53.383 | 133 | 62 | 0 | 9 | 141 | 9 | 141 | 5.14e-46 | 149 |
| Msa1209610 | MtrunA17_Chr3g0114061 | 66.019 | 103 | 35 | 0 | 10 | 112 | 7 | 109 | 8.11e-46 | 148 |
| Msa1209610 | MtrunA17_Chr5g0438091 | 62.385 | 109 | 41 | 0 | 10 | 118 | 38 | 146 | 6.31e-45 | 147 |
| Msa1209610 | MtrunA17_Chr3g0129071 | 61.468 | 109 | 42 | 0 | 10 | 118 | 46 | 154 | 1.51e-44 | 147 |
| Msa1209610 | MtrunA17_Chr3g0113891 | 63.000 | 100 | 37 | 0 | 9 | 108 | 12 | 111 | 2.13e-42 | 140 |
| Msa1209610 | MtrunA17_Chr5g0432451 | 58.015 | 131 | 54 | 1 | 10 | 140 | 42 | 171 | 4.68e-40 | 135 |
| Msa1209610 | MtrunA17_Chr4g0030001 | 53.448 | 116 | 53 | 1 | 2 | 116 | 15 | 130 | 7.29e-40 | 134 |
| Msa1209610 | MtrunA17_Chr7g0260971 | 59.130 | 115 | 43 | 2 | 1 | 111 | 1 | 115 | 3.85e-39 | 133 |
| Msa1209610 | MtrunA17_Chr1g0200201 | 58.416 | 101 | 42 | 0 | 12 | 112 | 73 | 173 | 1.29e-38 | 132 |
| Msa1209610 | MtrunA17_Chr8g0351701 | 57.944 | 107 | 44 | 1 | 11 | 116 | 23 | 129 | 1.66e-38 | 132 |
| Msa1209610 | MtrunA17_Chr8g0351721 | 54.918 | 122 | 52 | 2 | 11 | 131 | 18 | 137 | 4.56e-36 | 125 |
| Msa1209610 | MtrunA17_Chr1g0184281 | 62.105 | 95 | 35 | 1 | 11 | 104 | 15 | 109 | 8.50e-36 | 124 |
| Msa1209610 | MtrunA17_Chr7g0225111 | 51.376 | 109 | 53 | 0 | 10 | 118 | 4 | 112 | 9.94e-36 | 121 |
| Msa1209610 | MtrunA17_Chr7g0245871 | 62.105 | 95 | 35 | 1 | 11 | 104 | 27 | 121 | 2.87e-35 | 124 |
| Msa1209610 | MtrunA17_Chr6g0460951 | 51.852 | 108 | 51 | 1 | 10 | 116 | 39 | 146 | 4.10e-34 | 120 |
| Msa1209610 | MtrunA17_Chr1g0184271 | 37.572 | 173 | 98 | 3 | 11 | 177 | 12 | 180 | 4.39e-34 | 120 |
| Msa1209610 | MtrunA17_Chr5g0435641 | 52.475 | 101 | 47 | 1 | 6 | 105 | 2 | 102 | 5.02e-33 | 116 |
| Msa1209610 | MtrunA17_Chr8g0374601 | 48.077 | 104 | 54 | 0 | 11 | 114 | 26 | 129 | 1.07e-32 | 118 |
| Msa1209610 | MtrunA17_Chr7g0261031 | 58.333 | 96 | 39 | 1 | 10 | 104 | 7 | 102 | 1.17e-32 | 116 |
| Msa1209610 | MtrunA17_Chr5g0444351 | 48.598 | 107 | 55 | 0 | 12 | 118 | 6 | 112 | 5.23e-32 | 112 |
| Msa1209610 | MtrunA17_Chr8g0374591 | 48.077 | 104 | 54 | 0 | 11 | 114 | 8 | 111 | 1.78e-31 | 110 |
| Msa1209610 | MtrunA17_Chr7g0245861 | 60.000 | 90 | 33 | 2 | 11 | 97 | 12 | 101 | 1.44e-29 | 106 |
| Msa1209610 | MtrunA17_Chr4g0059901 | 48.958 | 96 | 49 | 0 | 12 | 107 | 11 | 106 | 2.43e-29 | 109 |
| Msa1209610 | MtrunA17_Chr8g0374581 | 46.667 | 105 | 55 | 1 | 11 | 114 | 8 | 112 | 2.05e-28 | 105 |
| Msa1209610 | MtrunA17_Chr6g0452661 | 44.898 | 98 | 53 | 1 | 9 | 105 | 7 | 104 | 6.39e-28 | 105 |
| Msa1209610 | MtrunA17_Chr6g0460941 | 48.315 | 89 | 45 | 1 | 11 | 98 | 7 | 95 | 2.02e-26 | 97.1 |
| Msa1209610 | MtrunA17_Chr8g0342351 | 43.000 | 100 | 57 | 0 | 12 | 111 | 13 | 112 | 5.92e-26 | 100 |
| Msa1209610 | MtrunA17_Chr6g0452671 | 36.220 | 127 | 72 | 2 | 12 | 130 | 12 | 137 | 1.10e-24 | 95.5 |
| Msa1209610 | MtrunA17_Chr6g0452691 | 49.398 | 83 | 41 | 1 | 9 | 90 | 7 | 89 | 1.94e-24 | 94.4 |
| Msa1209610 | MtrunA17_Chr4g0055441 | 40.385 | 104 | 60 | 2 | 12 | 114 | 3 | 105 | 9.66e-24 | 92.0 |
| Msa1209610 | MtrunA17_Chr2g0330201 | 43.011 | 93 | 53 | 0 | 12 | 104 | 20 | 112 | 3.97e-23 | 93.6 |
| Msa1209610 | MtrunA17_Chr4g0040831 | 39.423 | 104 | 63 | 0 | 1 | 104 | 5 | 108 | 8.05e-23 | 90.9 |
| Msa1209610 | MtrunA17_Chr5g0439111 | 40.187 | 107 | 62 | 2 | 9 | 114 | 7 | 112 | 1.70e-20 | 85.5 |
| Msa1209610 | MtrunA17_Chr2g0326041 | 60.784 | 51 | 20 | 0 | 10 | 60 | 32 | 82 | 5.88e-19 | 77.4 |
| Msa1209610 | MtrunA17_Chr4g0046201 | 35.556 | 90 | 58 | 0 | 10 | 99 | 5 | 94 | 4.85e-17 | 76.6 |
| Msa1209610 | MtrunA17_Chr2g0326051 | 54.098 | 61 | 28 | 0 | 56 | 116 | 5 | 65 | 6.97e-16 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1209610 | AT5G63090.4 | 68.333 | 180 | 45 | 3 | 1 | 171 | 1 | 177 | 1.78e-78 | 232 |
| Msa1209610 | AT5G63090.3 | 68.333 | 180 | 45 | 3 | 1 | 171 | 1 | 177 | 1.78e-78 | 232 |
| Msa1209610 | AT5G63090.2 | 68.333 | 180 | 45 | 3 | 1 | 171 | 1 | 177 | 1.78e-78 | 232 |
| Msa1209610 | AT5G63090.1 | 68.333 | 180 | 45 | 3 | 1 | 171 | 1 | 177 | 1.78e-78 | 232 |
| Msa1209610 | AT3G27650.1 | 78.182 | 110 | 24 | 0 | 5 | 114 | 33 | 142 | 3.30e-62 | 190 |
| Msa1209610 | AT1G65620.1 | 68.217 | 129 | 39 | 1 | 1 | 129 | 1 | 127 | 1.60e-52 | 167 |
| Msa1209610 | AT1G65620.4 | 68.217 | 129 | 39 | 1 | 1 | 129 | 1 | 127 | 1.60e-52 | 167 |
| Msa1209610 | AT1G65620.3 | 68.217 | 129 | 39 | 1 | 1 | 129 | 1 | 127 | 1.60e-52 | 167 |
| Msa1209610 | AT1G65620.5 | 68.217 | 129 | 39 | 1 | 1 | 129 | 1 | 127 | 1.60e-52 | 167 |
| Msa1209610 | AT1G65620.2 | 68.217 | 129 | 39 | 1 | 1 | 129 | 1 | 127 | 1.60e-52 | 167 |
| Msa1209610 | AT5G66870.1 | 60.839 | 143 | 45 | 3 | 1 | 142 | 1 | 133 | 7.72e-51 | 166 |
| Msa1209610 | AT2G23660.2 | 62.931 | 116 | 43 | 0 | 9 | 124 | 3 | 118 | 2.89e-49 | 162 |
| Msa1209610 | AT2G23660.3 | 62.931 | 116 | 43 | 0 | 9 | 124 | 3 | 118 | 2.89e-49 | 162 |
| Msa1209610 | AT2G23660.1 | 62.931 | 116 | 43 | 0 | 9 | 124 | 3 | 118 | 2.89e-49 | 162 |
| Msa1209610 | AT2G30130.1 | 64.286 | 112 | 40 | 0 | 9 | 120 | 6 | 117 | 3.59e-49 | 158 |
| Msa1209610 | AT1G31320.1 | 72.727 | 88 | 24 | 0 | 10 | 97 | 12 | 99 | 3.27e-44 | 145 |
| Msa1209610 | AT1G16530.1 | 59.829 | 117 | 45 | 2 | 10 | 124 | 13 | 129 | 1.66e-43 | 143 |
| Msa1209610 | AT1G07900.1 | 61.165 | 103 | 40 | 0 | 10 | 112 | 32 | 134 | 4.52e-43 | 142 |
| Msa1209610 | AT2G30340.1 | 60.952 | 105 | 41 | 0 | 10 | 114 | 51 | 155 | 1.17e-42 | 144 |
| Msa1209610 | AT2G30340.2 | 60.952 | 105 | 41 | 0 | 10 | 114 | 52 | 156 | 1.26e-42 | 144 |
| Msa1209610 | AT2G28500.1 | 58.879 | 107 | 44 | 0 | 10 | 116 | 54 | 160 | 2.48e-41 | 139 |
| Msa1209610 | AT3G11090.1 | 57.944 | 107 | 45 | 0 | 9 | 115 | 9 | 115 | 5.16e-41 | 136 |
| Msa1209610 | AT2G40470.2 | 63.810 | 105 | 38 | 0 | 10 | 114 | 19 | 123 | 9.19e-39 | 132 |
| Msa1209610 | AT2G40470.1 | 63.810 | 105 | 38 | 0 | 10 | 114 | 44 | 148 | 1.90e-38 | 132 |
| Msa1209610 | AT3G26660.1 | 55.000 | 100 | 45 | 0 | 12 | 111 | 6 | 105 | 1.09e-37 | 127 |
| Msa1209610 | AT3G26620.1 | 55.000 | 100 | 45 | 0 | 12 | 111 | 6 | 105 | 1.09e-37 | 127 |
| Msa1209610 | AT3G03760.1 | 56.731 | 104 | 44 | 1 | 10 | 112 | 50 | 153 | 2.42e-37 | 130 |
| Msa1209610 | AT4G00210.1 | 54.472 | 123 | 53 | 2 | 1 | 122 | 1 | 121 | 3.27e-37 | 129 |
| Msa1209610 | AT2G45420.1 | 63.043 | 92 | 33 | 1 | 14 | 104 | 40 | 131 | 1.55e-35 | 125 |
| Msa1209610 | AT4G00220.1 | 57.692 | 104 | 43 | 1 | 14 | 116 | 20 | 123 | 2.03e-35 | 124 |
| Msa1209610 | AT2G31310.1 | 38.503 | 187 | 84 | 5 | 10 | 170 | 6 | 187 | 5.95e-35 | 122 |
| Msa1209610 | AT2G42430.1 | 55.963 | 109 | 43 | 2 | 1 | 104 | 1 | 109 | 2.05e-34 | 122 |
| Msa1209610 | AT3G50510.1 | 45.902 | 122 | 64 | 1 | 10 | 129 | 11 | 132 | 1.00e-33 | 119 |
| Msa1209610 | AT3G50510.2 | 45.902 | 122 | 64 | 1 | 10 | 129 | 11 | 132 | 1.00e-33 | 119 |
| Msa1209610 | AT3G58190.1 | 56.250 | 96 | 41 | 1 | 11 | 105 | 11 | 106 | 3.51e-33 | 118 |
| Msa1209610 | AT2G45410.1 | 52.542 | 118 | 53 | 2 | 14 | 130 | 19 | 134 | 4.83e-33 | 117 |
| Msa1209610 | AT2G42440.1 | 53.608 | 97 | 44 | 1 | 10 | 105 | 6 | 102 | 2.48e-32 | 117 |
| Msa1209610 | AT5G06080.1 | 47.368 | 114 | 59 | 1 | 6 | 118 | 2 | 115 | 3.91e-32 | 114 |
| Msa1209610 | AT5G35900.1 | 49.000 | 100 | 51 | 0 | 12 | 111 | 6 | 105 | 1.11e-30 | 111 |
| Msa1209610 | AT3G13850.1 | 45.745 | 94 | 51 | 0 | 12 | 105 | 37 | 130 | 5.43e-28 | 106 |
| Msa1209610 | AT3G47870.1 | 41.748 | 103 | 60 | 0 | 12 | 114 | 37 | 139 | 5.01e-25 | 99.4 |
| Msa1209610 | AT1G72980.1 | 41.905 | 105 | 61 | 0 | 1 | 105 | 3 | 107 | 1.18e-23 | 93.6 |
| Msa1209610 | AT4G22700.1 | 39.640 | 111 | 65 | 2 | 12 | 121 | 6 | 115 | 7.01e-21 | 85.9 |
| Msa1209610 | AT1G06280.1 | 44.156 | 77 | 43 | 0 | 12 | 88 | 25 | 101 | 8.07e-18 | 78.2 |
| Msa1209610 | AT2G19820.1 | 34.694 | 98 | 63 | 1 | 2 | 98 | 3 | 100 | 2.73e-15 | 69.3 |
| Msa1209610 | AT5G15060.1 | 39.744 | 78 | 45 | 1 | 7 | 82 | 13 | 90 | 4.51e-15 | 69.3 |
| Msa1209610 | AT3G27940.3 | 38.806 | 67 | 41 | 0 | 10 | 76 | 4 | 70 | 1.17e-11 | 60.5 |
| Msa1209610 | AT3G27940.1 | 38.806 | 67 | 41 | 0 | 10 | 76 | 4 | 70 | 1.17e-11 | 60.5 |
| Msa1209610 | AT3G27940.2 | 38.806 | 67 | 41 | 0 | 10 | 76 | 4 | 70 | 1.17e-11 | 60.5 |
Find 55 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAACGTACACAAAATATT+TGG | 0.186655 | 8_1:+68195100 | Msa1209610:CDS |
| CTAAACTGTCTTTGTGGAAT+TGG | 0.275981 | 8_1:-68195391 | None:intergenic |
| CGAGTTAGGGATCCAGTTTA+TGG | 0.289758 | 8_1:+68195211 | Msa1209610:CDS |
| AGAGAGGCTGGAGGAGGTGG+AGG | 0.297872 | 8_1:-68195337 | None:intergenic |
| ACGAAGCCGAAGCACGAGTT+AGG | 0.305462 | 8_1:+68195197 | Msa1209610:CDS |
| CTTTACCTTATTCTCTTCCT+TGG | 0.333470 | 8_1:+68195464 | Msa1209610:CDS |
| ATGAGTGAAGGTGGAGGAGG+AGG | 0.339514 | 8_1:+68195508 | Msa1209610:CDS |
| TCAGTCCAAGGAAGAGAATA+AGG | 0.339719 | 8_1:-68195469 | None:intergenic |
| AGTGAAGGTGGAGGAGGAGG+AGG | 0.357862 | 8_1:+68195511 | Msa1209610:CDS |
| GAAGGTGGAGGAGGAGGAGG+AGG | 0.363314 | 8_1:+68195514 | Msa1209610:CDS |
| AAAGAAGAATAAGTAGTAGT+AGG | 0.401220 | 8_1:-68195445 | None:intergenic |
| GCTCCTACACATCCATAAAC+TGG | 0.405024 | 8_1:-68195223 | None:intergenic |
| GAAAGAGAGGCTGGAGGAGG+TGG | 0.409459 | 8_1:-68195340 | None:intergenic |
| CTTCCACTAAACTGTCTTTG+TGG | 0.427794 | 8_1:-68195397 | None:intergenic |
| GGCTCTTCTGGAGGGAAGTA+AGG | 0.432439 | 8_1:-68195067 | None:intergenic |
| TGGAGGAGGTGGAGGGATAT+CGG | 0.435988 | 8_1:-68195329 | None:intergenic |
| AGTTAACTGCATCCTCCCTT+TGG | 0.438074 | 8_1:-68195165 | None:intergenic |
| GATATGAGTGAAGGTGGAGG+AGG | 0.442790 | 8_1:+68195505 | Msa1209610:CDS |
| ATTCCACAAAGACAGTTTAG+TGG | 0.446210 | 8_1:+68195394 | Msa1209610:CDS |
| TGGATCCCTAACTCGTGCTT+CGG | 0.456667 | 8_1:-68195203 | None:intergenic |
| CAGTTTAGTGGAAGAAATGA+AGG | 0.473637 | 8_1:+68195406 | Msa1209610:CDS |
| TGAGGCACAGAAAGAGAGGC+TGG | 0.482119 | 8_1:-68195349 | None:intergenic |
| ACTGTCTTTGTGGAATTGGT+TGG | 0.483148 | 8_1:-68195387 | None:intergenic |
| TTGAGGAGAAAATGCATGCC+AGG | 0.496470 | 8_1:+68195031 | Msa1209610:CDS |
| ACAAGTTCAAAGGCTTCAAA+AGG | 0.500774 | 8_1:+68195264 | Msa1209610:CDS |
| CTGCATCCTCCCTTTGGTGA+GGG | 0.501198 | 8_1:-68195159 | None:intergenic |
| GCCTCTCTTTCTGTGCCTCA+AGG | 0.505853 | 8_1:+68195352 | Msa1209610:CDS |
| CGAAGCCGAAGCACGAGTTA+GGG | 0.512333 | 8_1:+68195198 | Msa1209610:CDS |
| GAGAGGCTGGAGGAGGTGGA+GGG | 0.516068 | 8_1:-68195336 | None:intergenic |
| CATCCTCCCTTTGGTGAGGG+AGG | 0.527585 | 8_1:-68195156 | None:intergenic |
| TTCTACAAAGACAAGTTCAA+AGG | 0.528529 | 8_1:+68195254 | Msa1209610:CDS |
| GAGGTGGAGGGATATCGGTG+AGG | 0.544709 | 8_1:-68195324 | None:intergenic |
| AGGTGGAGGGATATCGGTGA+GGG | 0.546572 | 8_1:-68195323 | None:intergenic |
| GTGCTGCCTGCAAATTCTTG+AGG | 0.549706 | 8_1:+68195014 | Msa1209610:CDS |
| ACAGAAAGAGAGGCTGGAGG+AGG | 0.555018 | 8_1:-68195343 | None:intergenic |
| CGAACTCCTCCCTCACCAAA+GGG | 0.555403 | 8_1:+68195150 | Msa1209610:CDS |
| ACTGCATCCTCCCTTTGGTG+AGG | 0.557366 | 8_1:-68195160 | None:intergenic |
| TTGTCTTTGTAGAAAAGAGA+TGG | 0.561057 | 8_1:-68195245 | None:intergenic |
| GAGGGTATAGCGAAGAAGAT+CGG | 0.563089 | 8_1:-68195305 | None:intergenic |
| AAGAATTTGCAGGCAGCACA+AGG | 0.573114 | 8_1:-68195010 | None:intergenic |
| ACGAACTCCTCCCTCACCAA+AGG | 0.580542 | 8_1:+68195149 | Msa1209610:CDS |
| GATCCAGTTTATGGATGTGT+AGG | 0.582057 | 8_1:+68195220 | Msa1209610:CDS |
| TCCTTGAGGCACAGAAAGAG+AGG | 0.595065 | 8_1:-68195353 | None:intergenic |
| TCCTCAGTGGATATGAGTGA+AGG | 0.615767 | 8_1:+68195496 | Msa1209610:CDS |
| TAAGGTGCAAAGATGCAACC+TGG | 0.617157 | 8_1:-68195049 | None:intergenic |
| TCAGTGGATATGAGTGAAGG+TGG | 0.618537 | 8_1:+68195499 | Msa1209610:CDS |
| GTGGATATGAGTGAAGGTGG+AGG | 0.633891 | 8_1:+68195502 | Msa1209610:CDS |
| ACTGAGGAAGTATCAGTCCA+AGG | 0.638918 | 8_1:-68195481 | None:intergenic |
| TTGGACTGATACTTCCTCAG+TGG | 0.639081 | 8_1:+68195483 | Msa1209610:CDS |
| AGTTTAGTGGAAGAAATGAA+GGG | 0.640159 | 8_1:+68195407 | Msa1209610:CDS |
| ACAAGGTGAATTGTATGCGC+TGG | 0.642277 | 8_1:-68194993 | None:intergenic |
| TGGACTGATATCATTCCTTG+AGG | 0.655168 | 8_1:-68195367 | None:intergenic |
| ACTCCTCCCTCACCAAAGGG+AGG | 0.660217 | 8_1:+68195153 | Msa1209610:CDS |
| GGCACAGAAAGAGAGGCTGG+AGG | 0.697106 | 8_1:-68195346 | None:intergenic |
| ACCTTCACTCATATCCACTG+AGG | 0.758906 | 8_1:-68195497 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGAAGAATAAGTAGTAGT+AGG | - | chr8_1:68195448-68195467 | None:intergenic | 25.0% |
| ! | TGTACGTTTGCAAATTTTTG+AGG | - | chr8_1:68195091-68195110 | None:intergenic | 30.0% |
| GCAAACGTACACAAAATATT+TGG | + | chr8_1:68195100-68195119 | Msa1209610:CDS | 30.0% | |
| TTGTCTTTGTAGAAAAGAGA+TGG | - | chr8_1:68195248-68195267 | None:intergenic | 30.0% | |
| TTCTACAAAGACAAGTTCAA+AGG | + | chr8_1:68195254-68195273 | Msa1209610:CDS | 30.0% | |
| AGTTTAGTGGAAGAAATGAA+GGG | + | chr8_1:68195407-68195426 | Msa1209610:CDS | 30.0% | |
| ! | TTTTCTCCTCAAGAATTTGC+AGG | - | chr8_1:68195023-68195042 | None:intergenic | 35.0% |
| ACAAGTTCAAAGGCTTCAAA+AGG | + | chr8_1:68195264-68195283 | Msa1209610:CDS | 35.0% | |
| CTAAACTGTCTTTGTGGAAT+TGG | - | chr8_1:68195394-68195413 | None:intergenic | 35.0% | |
| ATTCCACAAAGACAGTTTAG+TGG | + | chr8_1:68195394-68195413 | Msa1209610:CDS | 35.0% | |
| CAGTTTAGTGGAAGAAATGA+AGG | + | chr8_1:68195406-68195425 | Msa1209610:CDS | 35.0% | |
| CTTTACCTTATTCTCTTCCT+TGG | + | chr8_1:68195464-68195483 | Msa1209610:CDS | 35.0% | |
| !!! | ATTTTTGAGGCTCTTCTGGA+GGG | - | chr8_1:68195078-68195097 | None:intergenic | 40.0% |
| !!! | AATTTTTGAGGCTCTTCTGG+AGG | - | chr8_1:68195079-68195098 | None:intergenic | 40.0% |
| !!! | GCAAATTTTTGAGGCTCTTC+TGG | - | chr8_1:68195082-68195101 | None:intergenic | 40.0% |
| GATCCAGTTTATGGATGTGT+AGG | + | chr8_1:68195220-68195239 | Msa1209610:CDS | 40.0% | |
| TGGACTGATATCATTCCTTG+AGG | - | chr8_1:68195370-68195389 | None:intergenic | 40.0% | |
| ACTGTCTTTGTGGAATTGGT+TGG | - | chr8_1:68195390-68195409 | None:intergenic | 40.0% | |
| CTTCCACTAAACTGTCTTTG+TGG | - | chr8_1:68195400-68195419 | None:intergenic | 40.0% | |
| TCAGTCCAAGGAAGAGAATA+AGG | - | chr8_1:68195472-68195491 | None:intergenic | 40.0% | |
| ACAAGGTGAATTGTATGCGC+TGG | - | chr8_1:68194996-68195015 | None:intergenic | 45.0% | |
| AAGAATTTGCAGGCAGCACA+AGG | - | chr8_1:68195013-68195032 | None:intergenic | 45.0% | |
| TTGAGGAGAAAATGCATGCC+AGG | + | chr8_1:68195031-68195050 | Msa1209610:CDS | 45.0% | |
| TAAGGTGCAAAGATGCAACC+TGG | - | chr8_1:68195052-68195071 | None:intergenic | 45.0% | |
| AGTTAACTGCATCCTCCCTT+TGG | - | chr8_1:68195168-68195187 | None:intergenic | 45.0% | |
| CGAGTTAGGGATCCAGTTTA+TGG | + | chr8_1:68195211-68195230 | Msa1209610:CDS | 45.0% | |
| GCTCCTACACATCCATAAAC+TGG | - | chr8_1:68195226-68195245 | None:intergenic | 45.0% | |
| ! | GAGGGTATAGCGAAGAAGAT+CGG | - | chr8_1:68195308-68195327 | None:intergenic | 45.0% |
| !! | ACTGAGGAAGTATCAGTCCA+AGG | - | chr8_1:68195484-68195503 | None:intergenic | 45.0% |
| TTGGACTGATACTTCCTCAG+TGG | + | chr8_1:68195483-68195502 | Msa1209610:CDS | 45.0% | |
| TCCTCAGTGGATATGAGTGA+AGG | + | chr8_1:68195496-68195515 | Msa1209610:CDS | 45.0% | |
| ACCTTCACTCATATCCACTG+AGG | - | chr8_1:68195500-68195519 | None:intergenic | 45.0% | |
| TCAGTGGATATGAGTGAAGG+TGG | + | chr8_1:68195499-68195518 | Msa1209610:CDS | 45.0% | |
| GTGCTGCCTGCAAATTCTTG+AGG | + | chr8_1:68195014-68195033 | Msa1209610:CDS | 50.0% | |
| TGGATCCCTAACTCGTGCTT+CGG | - | chr8_1:68195206-68195225 | None:intergenic | 50.0% | |
| TCCTTGAGGCACAGAAAGAG+AGG | - | chr8_1:68195356-68195375 | None:intergenic | 50.0% | |
| GTGGATATGAGTGAAGGTGG+AGG | + | chr8_1:68195502-68195521 | Msa1209610:CDS | 50.0% | |
| GATATGAGTGAAGGTGGAGG+AGG | + | chr8_1:68195505-68195524 | Msa1209610:CDS | 50.0% | |
| ! | GGCTCTTCTGGAGGGAAGTA+AGG | - | chr8_1:68195070-68195089 | None:intergenic | 55.0% |
| ACGAACTCCTCCCTCACCAA+AGG | + | chr8_1:68195149-68195168 | Msa1209610:CDS | 55.0% | |
| CGAACTCCTCCCTCACCAAA+GGG | + | chr8_1:68195150-68195169 | Msa1209610:CDS | 55.0% | |
| CTGCATCCTCCCTTTGGTGA+GGG | - | chr8_1:68195162-68195181 | None:intergenic | 55.0% | |
| ACTGCATCCTCCCTTTGGTG+AGG | - | chr8_1:68195163-68195182 | None:intergenic | 55.0% | |
| ACGAAGCCGAAGCACGAGTT+AGG | + | chr8_1:68195197-68195216 | Msa1209610:CDS | 55.0% | |
| CGAAGCCGAAGCACGAGTTA+GGG | + | chr8_1:68195198-68195217 | Msa1209610:CDS | 55.0% | |
| AGGTGGAGGGATATCGGTGA+GGG | - | chr8_1:68195326-68195345 | None:intergenic | 55.0% | |
| TGGAGGAGGTGGAGGGATAT+CGG | - | chr8_1:68195332-68195351 | None:intergenic | 55.0% | |
| ACAGAAAGAGAGGCTGGAGG+AGG | - | chr8_1:68195346-68195365 | None:intergenic | 55.0% | |
| TGAGGCACAGAAAGAGAGGC+TGG | - | chr8_1:68195352-68195371 | None:intergenic | 55.0% | |
| GCCTCTCTTTCTGTGCCTCA+AGG | + | chr8_1:68195352-68195371 | Msa1209610:CDS | 55.0% | |
| ATGAGTGAAGGTGGAGGAGG+AGG | + | chr8_1:68195508-68195527 | Msa1209610:CDS | 55.0% | |
| ACTCCTCCCTCACCAAAGGG+AGG | + | chr8_1:68195153-68195172 | Msa1209610:CDS | 60.0% | |
| CATCCTCCCTTTGGTGAGGG+AGG | - | chr8_1:68195159-68195178 | None:intergenic | 60.0% | |
| GAGGTGGAGGGATATCGGTG+AGG | - | chr8_1:68195327-68195346 | None:intergenic | 60.0% | |
| GAAAGAGAGGCTGGAGGAGG+TGG | - | chr8_1:68195343-68195362 | None:intergenic | 60.0% | |
| GGCACAGAAAGAGAGGCTGG+AGG | - | chr8_1:68195349-68195368 | None:intergenic | 60.0% | |
| AGTGAAGGTGGAGGAGGAGG+AGG | + | chr8_1:68195511-68195530 | Msa1209610:CDS | 60.0% | |
| GAGAGGCTGGAGGAGGTGGA+GGG | - | chr8_1:68195339-68195358 | None:intergenic | 65.0% | |
| AGAGAGGCTGGAGGAGGTGG+AGG | - | chr8_1:68195340-68195359 | None:intergenic | 65.0% | |
| GAAGGTGGAGGAGGAGGAGG+AGG | + | chr8_1:68195514-68195533 | Msa1209610:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 68194980 | 68195552 | 68194980 | ID=Msa1209610;Name=Msa1209610 |
| chr8_1 | mRNA | 68194980 | 68195552 | 68194980 | ID=Msa1209610-mRNA-1;Parent=Msa1209610;Name=Msa1209610-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0|-1|0|1|-1|1|1|0|190 |
| chr8_1 | exon | 68194980 | 68195552 | 68194980 | ID=Msa1209610-mRNA-1:exon:22660;Parent=Msa1209610-mRNA-1 |
| chr8_1 | CDS | 68194980 | 68195552 | 68194980 | ID=Msa1209610-mRNA-1:cds;Parent=Msa1209610-mRNA-1 |
| Gene Sequence |
| Protein sequence |
