Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1246080 | XP_013457768.1 | 96.296 | 189 | 6 | 1 | 1 | 189 | 1 | 188 | 1.05e-131 | 377 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1246080 | sp|Q9FML4|LOB_ARATH | 68.333 | 180 | 45 | 3 | 1 | 171 | 1 | 177 | 1.82e-77 | 232 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa1246080 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1246080 | Msa1298850 | 0.930374 | 1.797903e-93 | -8.615850e-47 |
| Msa1246080 | Msa1330510 | 0.811756 | 5.972063e-51 | -8.615850e-47 |
| Msa1209610 | Msa1246080 | 0.946277 | 6.268399e-105 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1246080 | MtrunA17_Chr4g0058651 | 96.296 | 189 | 6 | 1 | 1 | 189 | 1 | 188 | 9.66e-136 | 377 |
| Msa1246080 | MtrunA17_Chr3g0117121 | 72.881 | 177 | 46 | 2 | 3 | 178 | 2 | 177 | 9.36e-86 | 251 |
| Msa1246080 | MtrunA17_Chr7g0227511 | 61.376 | 189 | 56 | 4 | 1 | 189 | 1 | 172 | 8.29e-73 | 217 |
| Msa1246080 | MtrunA17_Chr6g0449641 | 82.301 | 113 | 18 | 1 | 1 | 113 | 1 | 111 | 2.26e-65 | 197 |
| Msa1246080 | MtrunA17_Chr6g0449651 | 79.130 | 115 | 22 | 1 | 1 | 115 | 59 | 171 | 2.11e-63 | 194 |
| Msa1246080 | MtrunA17_Chr8g0353981 | 66.917 | 133 | 40 | 1 | 1 | 133 | 1 | 129 | 1.08e-53 | 171 |
| Msa1246080 | MtrunA17_Chr5g0437571 | 64.957 | 117 | 38 | 1 | 9 | 122 | 4 | 120 | 1.37e-50 | 160 |
| Msa1246080 | MtrunA17_Chr5g0402611 | 69.091 | 110 | 34 | 0 | 5 | 114 | 2 | 111 | 3.83e-50 | 164 |
| Msa1246080 | MtrunA17_Chr3g0115431 | 64.754 | 122 | 43 | 0 | 5 | 126 | 3 | 124 | 4.43e-50 | 161 |
| Msa1246080 | MtrunA17_Chr3g0097221 | 65.179 | 112 | 39 | 0 | 6 | 117 | 2 | 113 | 3.76e-48 | 158 |
| Msa1246080 | MtrunA17_Chr2g0311601 | 63.551 | 107 | 39 | 0 | 10 | 116 | 12 | 118 | 8.63e-48 | 153 |
| Msa1246080 | MtrunA17_Chr4g0043421 | 65.385 | 104 | 36 | 0 | 10 | 113 | 14 | 117 | 2.57e-47 | 152 |
| Msa1246080 | MtrunA17_Chr3g0106101 | 64.423 | 104 | 37 | 0 | 9 | 112 | 5 | 108 | 6.93e-47 | 151 |
| Msa1246080 | MtrunA17_Chr5g0437401 | 53.383 | 133 | 62 | 0 | 9 | 141 | 9 | 141 | 4.55e-46 | 149 |
| Msa1246080 | MtrunA17_Chr3g0114061 | 66.019 | 103 | 35 | 0 | 10 | 112 | 7 | 109 | 6.65e-46 | 148 |
| Msa1246080 | MtrunA17_Chr5g0438091 | 62.385 | 109 | 41 | 0 | 10 | 118 | 38 | 146 | 5.96e-45 | 147 |
| Msa1246080 | MtrunA17_Chr3g0129071 | 61.468 | 109 | 42 | 0 | 10 | 118 | 46 | 154 | 1.54e-44 | 147 |
| Msa1246080 | MtrunA17_Chr3g0113891 | 63.000 | 100 | 37 | 0 | 9 | 108 | 12 | 111 | 1.93e-42 | 140 |
| Msa1246080 | MtrunA17_Chr5g0432451 | 58.015 | 131 | 54 | 1 | 10 | 140 | 42 | 171 | 4.78e-40 | 135 |
| Msa1246080 | MtrunA17_Chr4g0030001 | 53.448 | 116 | 53 | 1 | 2 | 116 | 15 | 130 | 6.12e-40 | 134 |
| Msa1246080 | MtrunA17_Chr7g0260971 | 59.130 | 115 | 43 | 2 | 1 | 111 | 1 | 115 | 3.41e-39 | 133 |
| Msa1246080 | MtrunA17_Chr1g0200201 | 58.416 | 101 | 42 | 0 | 12 | 112 | 73 | 173 | 1.28e-38 | 132 |
| Msa1246080 | MtrunA17_Chr8g0351701 | 57.944 | 107 | 44 | 1 | 11 | 116 | 23 | 129 | 1.52e-38 | 132 |
| Msa1246080 | MtrunA17_Chr8g0351721 | 54.918 | 122 | 52 | 2 | 11 | 131 | 18 | 137 | 4.27e-36 | 125 |
| Msa1246080 | MtrunA17_Chr1g0184281 | 62.105 | 95 | 35 | 1 | 11 | 104 | 15 | 109 | 7.55e-36 | 124 |
| Msa1246080 | MtrunA17_Chr7g0225111 | 51.376 | 109 | 53 | 0 | 10 | 118 | 4 | 112 | 9.42e-36 | 121 |
| Msa1246080 | MtrunA17_Chr7g0245871 | 62.105 | 95 | 35 | 1 | 11 | 104 | 27 | 121 | 2.45e-35 | 124 |
| Msa1246080 | MtrunA17_Chr6g0460951 | 51.852 | 108 | 51 | 1 | 10 | 116 | 39 | 146 | 4.06e-34 | 120 |
| Msa1246080 | MtrunA17_Chr1g0184271 | 37.572 | 173 | 98 | 3 | 11 | 177 | 12 | 180 | 4.25e-34 | 120 |
| Msa1246080 | MtrunA17_Chr5g0435641 | 52.475 | 101 | 47 | 1 | 6 | 105 | 2 | 102 | 4.61e-33 | 117 |
| Msa1246080 | MtrunA17_Chr7g0261031 | 58.333 | 96 | 39 | 1 | 10 | 104 | 7 | 102 | 1.01e-32 | 117 |
| Msa1246080 | MtrunA17_Chr8g0374601 | 48.077 | 104 | 54 | 0 | 11 | 114 | 26 | 129 | 1.10e-32 | 118 |
| Msa1246080 | MtrunA17_Chr5g0444351 | 48.598 | 107 | 55 | 0 | 12 | 118 | 6 | 112 | 4.85e-32 | 112 |
| Msa1246080 | MtrunA17_Chr8g0374591 | 48.077 | 104 | 54 | 0 | 11 | 114 | 8 | 111 | 1.60e-31 | 110 |
| Msa1246080 | MtrunA17_Chr7g0245861 | 60.000 | 90 | 33 | 2 | 11 | 97 | 12 | 101 | 1.38e-29 | 106 |
| Msa1246080 | MtrunA17_Chr4g0059901 | 48.958 | 96 | 49 | 0 | 12 | 107 | 11 | 106 | 2.38e-29 | 109 |
| Msa1246080 | MtrunA17_Chr8g0374581 | 46.667 | 105 | 55 | 1 | 11 | 114 | 8 | 112 | 2.04e-28 | 105 |
| Msa1246080 | MtrunA17_Chr6g0452661 | 44.898 | 98 | 53 | 1 | 9 | 105 | 7 | 104 | 6.54e-28 | 105 |
| Msa1246080 | MtrunA17_Chr6g0460941 | 48.315 | 89 | 45 | 1 | 11 | 98 | 7 | 95 | 1.94e-26 | 97.1 |
| Msa1246080 | MtrunA17_Chr8g0342351 | 43.000 | 100 | 57 | 0 | 12 | 111 | 13 | 112 | 5.39e-26 | 100 |
| Msa1246080 | MtrunA17_Chr6g0452671 | 36.220 | 127 | 72 | 2 | 12 | 130 | 12 | 137 | 1.14e-24 | 95.5 |
| Msa1246080 | MtrunA17_Chr6g0452691 | 49.398 | 83 | 41 | 1 | 9 | 90 | 7 | 89 | 1.77e-24 | 94.4 |
| Msa1246080 | MtrunA17_Chr4g0055441 | 40.385 | 104 | 60 | 2 | 12 | 114 | 3 | 105 | 8.81e-24 | 92.0 |
| Msa1246080 | MtrunA17_Chr2g0330201 | 43.011 | 93 | 53 | 0 | 12 | 104 | 20 | 112 | 4.51e-23 | 93.6 |
| Msa1246080 | MtrunA17_Chr4g0040831 | 39.423 | 104 | 63 | 0 | 1 | 104 | 5 | 108 | 8.34e-23 | 90.5 |
| Msa1246080 | MtrunA17_Chr5g0439111 | 40.187 | 107 | 62 | 2 | 9 | 114 | 7 | 112 | 1.66e-20 | 85.5 |
| Msa1246080 | MtrunA17_Chr2g0326041 | 60.784 | 51 | 20 | 0 | 10 | 60 | 32 | 82 | 6.11e-19 | 77.4 |
| Msa1246080 | MtrunA17_Chr4g0046201 | 35.556 | 90 | 58 | 0 | 10 | 99 | 5 | 94 | 4.93e-17 | 76.6 |
| Msa1246080 | MtrunA17_Chr2g0326051 | 54.098 | 61 | 28 | 0 | 56 | 116 | 5 | 65 | 6.12e-16 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1246080 | AT5G63090.4 | 68.333 | 180 | 45 | 3 | 1 | 171 | 1 | 177 | 1.85e-78 | 232 |
| Msa1246080 | AT5G63090.3 | 68.333 | 180 | 45 | 3 | 1 | 171 | 1 | 177 | 1.85e-78 | 232 |
| Msa1246080 | AT5G63090.2 | 68.333 | 180 | 45 | 3 | 1 | 171 | 1 | 177 | 1.85e-78 | 232 |
| Msa1246080 | AT5G63090.1 | 68.333 | 180 | 45 | 3 | 1 | 171 | 1 | 177 | 1.85e-78 | 232 |
| Msa1246080 | AT3G27650.1 | 78.182 | 110 | 24 | 0 | 5 | 114 | 33 | 142 | 2.91e-62 | 190 |
| Msa1246080 | AT1G65620.1 | 68.217 | 129 | 39 | 1 | 1 | 129 | 1 | 127 | 1.66e-52 | 167 |
| Msa1246080 | AT1G65620.4 | 68.217 | 129 | 39 | 1 | 1 | 129 | 1 | 127 | 1.66e-52 | 167 |
| Msa1246080 | AT1G65620.3 | 68.217 | 129 | 39 | 1 | 1 | 129 | 1 | 127 | 1.66e-52 | 167 |
| Msa1246080 | AT1G65620.5 | 68.217 | 129 | 39 | 1 | 1 | 129 | 1 | 127 | 1.66e-52 | 167 |
| Msa1246080 | AT1G65620.2 | 68.217 | 129 | 39 | 1 | 1 | 129 | 1 | 127 | 1.66e-52 | 167 |
| Msa1246080 | AT5G66870.1 | 60.839 | 143 | 45 | 3 | 1 | 142 | 1 | 133 | 8.03e-51 | 166 |
| Msa1246080 | AT2G23660.2 | 62.931 | 116 | 43 | 0 | 9 | 124 | 3 | 118 | 3.11e-49 | 162 |
| Msa1246080 | AT2G23660.3 | 62.931 | 116 | 43 | 0 | 9 | 124 | 3 | 118 | 3.11e-49 | 162 |
| Msa1246080 | AT2G23660.1 | 62.931 | 116 | 43 | 0 | 9 | 124 | 3 | 118 | 3.11e-49 | 162 |
| Msa1246080 | AT2G30130.1 | 64.286 | 112 | 40 | 0 | 9 | 120 | 6 | 117 | 3.39e-49 | 158 |
| Msa1246080 | AT1G31320.1 | 72.727 | 88 | 24 | 0 | 10 | 97 | 12 | 99 | 3.37e-44 | 145 |
| Msa1246080 | AT1G16530.1 | 59.829 | 117 | 45 | 2 | 10 | 124 | 13 | 129 | 1.54e-43 | 143 |
| Msa1246080 | AT1G07900.1 | 61.165 | 103 | 40 | 0 | 10 | 112 | 32 | 134 | 4.23e-43 | 142 |
| Msa1246080 | AT2G30340.2 | 60.952 | 105 | 41 | 0 | 10 | 114 | 52 | 156 | 1.21e-42 | 144 |
| Msa1246080 | AT2G30340.1 | 60.952 | 105 | 41 | 0 | 10 | 114 | 51 | 155 | 1.27e-42 | 144 |
| Msa1246080 | AT2G28500.1 | 58.879 | 107 | 44 | 0 | 10 | 116 | 54 | 160 | 2.42e-41 | 139 |
| Msa1246080 | AT3G11090.1 | 57.944 | 107 | 45 | 0 | 9 | 115 | 9 | 115 | 4.72e-41 | 136 |
| Msa1246080 | AT2G40470.2 | 63.810 | 105 | 38 | 0 | 10 | 114 | 19 | 123 | 8.52e-39 | 132 |
| Msa1246080 | AT2G40470.1 | 63.810 | 105 | 38 | 0 | 10 | 114 | 44 | 148 | 1.76e-38 | 132 |
| Msa1246080 | AT3G26660.1 | 55.000 | 100 | 45 | 0 | 12 | 111 | 6 | 105 | 9.76e-38 | 127 |
| Msa1246080 | AT3G26620.1 | 55.000 | 100 | 45 | 0 | 12 | 111 | 6 | 105 | 1.04e-37 | 127 |
| Msa1246080 | AT3G03760.1 | 56.731 | 104 | 44 | 1 | 10 | 112 | 50 | 153 | 2.00e-37 | 130 |
| Msa1246080 | AT4G00210.1 | 54.472 | 123 | 53 | 2 | 1 | 122 | 1 | 121 | 3.57e-37 | 128 |
| Msa1246080 | AT2G45420.1 | 63.043 | 92 | 33 | 1 | 14 | 104 | 40 | 131 | 1.43e-35 | 125 |
| Msa1246080 | AT4G00220.1 | 57.692 | 104 | 43 | 1 | 14 | 116 | 20 | 123 | 2.05e-35 | 124 |
| Msa1246080 | AT2G31310.1 | 38.503 | 187 | 84 | 5 | 10 | 170 | 6 | 187 | 6.14e-35 | 122 |
| Msa1246080 | AT2G42430.1 | 55.963 | 109 | 43 | 2 | 1 | 104 | 1 | 109 | 2.03e-34 | 122 |
| Msa1246080 | AT3G50510.1 | 45.902 | 122 | 64 | 1 | 10 | 129 | 11 | 132 | 9.32e-34 | 119 |
| Msa1246080 | AT3G50510.2 | 45.902 | 122 | 64 | 1 | 10 | 129 | 11 | 132 | 9.32e-34 | 119 |
| Msa1246080 | AT3G58190.1 | 56.250 | 96 | 41 | 1 | 11 | 105 | 11 | 106 | 3.22e-33 | 118 |
| Msa1246080 | AT2G45410.1 | 52.542 | 118 | 53 | 2 | 14 | 130 | 19 | 134 | 4.88e-33 | 117 |
| Msa1246080 | AT2G42440.1 | 53.608 | 97 | 44 | 1 | 10 | 105 | 6 | 102 | 2.07e-32 | 117 |
| Msa1246080 | AT5G06080.1 | 47.368 | 114 | 59 | 1 | 6 | 118 | 2 | 115 | 3.83e-32 | 114 |
| Msa1246080 | AT5G35900.1 | 49.000 | 100 | 51 | 0 | 12 | 111 | 6 | 105 | 1.16e-30 | 111 |
| Msa1246080 | AT3G13850.1 | 45.745 | 94 | 51 | 0 | 12 | 105 | 37 | 130 | 5.38e-28 | 106 |
| Msa1246080 | AT3G47870.1 | 41.748 | 103 | 60 | 0 | 12 | 114 | 37 | 139 | 4.39e-25 | 99.8 |
| Msa1246080 | AT1G72980.1 | 41.905 | 105 | 61 | 0 | 1 | 105 | 3 | 107 | 1.21e-23 | 93.6 |
| Msa1246080 | AT4G22700.1 | 39.640 | 111 | 65 | 2 | 12 | 121 | 6 | 115 | 6.20e-21 | 85.9 |
| Msa1246080 | AT1G06280.1 | 44.156 | 77 | 43 | 0 | 12 | 88 | 25 | 101 | 7.71e-18 | 78.2 |
| Msa1246080 | AT2G19820.1 | 34.694 | 98 | 63 | 1 | 2 | 98 | 3 | 100 | 3.06e-15 | 69.3 |
| Msa1246080 | AT5G15060.1 | 39.744 | 78 | 45 | 1 | 7 | 82 | 13 | 90 | 4.84e-15 | 68.9 |
| Msa1246080 | AT3G27940.3 | 38.806 | 67 | 41 | 0 | 10 | 76 | 4 | 70 | 1.07e-11 | 60.5 |
| Msa1246080 | AT3G27940.1 | 38.806 | 67 | 41 | 0 | 10 | 76 | 4 | 70 | 1.07e-11 | 60.5 |
| Msa1246080 | AT3G27940.2 | 38.806 | 67 | 41 | 0 | 10 | 76 | 4 | 70 | 1.07e-11 | 60.5 |
Find 55 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAACGTACACAAAATATT+TGG | 0.186655 | 8_2:+36449653 | Msa1246080:CDS |
| CTAAACTGTCTTTGTGGAAT+TGG | 0.275981 | 8_2:-36449944 | None:intergenic |
| CGAGTTAGGGATCCAGTTTA+TGG | 0.289758 | 8_2:+36449764 | Msa1246080:CDS |
| AGAGAGGCTGGAGGAGGTGG+AGG | 0.297872 | 8_2:-36449890 | None:intergenic |
| ACGAAGCCGAAGCACGAGTT+AGG | 0.305462 | 8_2:+36449750 | Msa1246080:CDS |
| CTTTACCTTATTCTCTTCCT+TGG | 0.333470 | 8_2:+36450017 | Msa1246080:CDS |
| ATGAGTGAAGGTGGAGGAGG+AGG | 0.339514 | 8_2:+36450061 | Msa1246080:CDS |
| TCAGTCCAAGGAAGAGAATA+AGG | 0.339719 | 8_2:-36450022 | None:intergenic |
| AGTGAAGGTGGAGGAGGAGG+AGG | 0.357862 | 8_2:+36450064 | Msa1246080:CDS |
| GAAGGTGGAGGAGGAGGAGG+AGG | 0.363314 | 8_2:+36450067 | Msa1246080:CDS |
| AAAGAAGAATAAGTAGTAGT+AGG | 0.401220 | 8_2:-36449998 | None:intergenic |
| GCTCCTACACATCCATAAAC+TGG | 0.405024 | 8_2:-36449776 | None:intergenic |
| GAAAGAGAGGCTGGAGGAGG+TGG | 0.409459 | 8_2:-36449893 | None:intergenic |
| CTTCCACTAAACTGTCTTTG+TGG | 0.427794 | 8_2:-36449950 | None:intergenic |
| GGCTCTTCTGGAGGGAAGTA+AGG | 0.432439 | 8_2:-36449620 | None:intergenic |
| TGGAGGAGGTGGAGGGATAT+CGG | 0.435988 | 8_2:-36449882 | None:intergenic |
| AGTTAACTGCATCCTCCCTT+TGG | 0.438074 | 8_2:-36449718 | None:intergenic |
| GATATGAGTGAAGGTGGAGG+AGG | 0.442790 | 8_2:+36450058 | Msa1246080:CDS |
| ATTCCACAAAGACAGTTTAG+TGG | 0.446210 | 8_2:+36449947 | Msa1246080:CDS |
| TGGATCCCTAACTCGTGCTT+CGG | 0.456667 | 8_2:-36449756 | None:intergenic |
| CAGTTTAGTGGAAGAAATGA+AGG | 0.473637 | 8_2:+36449959 | Msa1246080:CDS |
| TGAGGCACAGAAAGAGAGGC+TGG | 0.482119 | 8_2:-36449902 | None:intergenic |
| ACTGTCTTTGTGGAATTGGT+TGG | 0.483148 | 8_2:-36449940 | None:intergenic |
| TTGAGGAGAAAATGCATGCC+AGG | 0.496470 | 8_2:+36449584 | Msa1246080:CDS |
| ACAAGTTCAAAGGCTTCAAA+AGG | 0.500774 | 8_2:+36449817 | Msa1246080:CDS |
| CTGCATCCTCCCTTTGGTGA+GGG | 0.501198 | 8_2:-36449712 | None:intergenic |
| GCCTCTCTTTCTGTGCCTCA+AGG | 0.505853 | 8_2:+36449905 | Msa1246080:CDS |
| CGAAGCCGAAGCACGAGTTA+GGG | 0.512333 | 8_2:+36449751 | Msa1246080:CDS |
| GAGAGGCTGGAGGAGGTGGA+GGG | 0.516068 | 8_2:-36449889 | None:intergenic |
| CATCCTCCCTTTGGTGAGGG+AGG | 0.527585 | 8_2:-36449709 | None:intergenic |
| TTCTACAAAGACAAGTTCAA+AGG | 0.528529 | 8_2:+36449807 | Msa1246080:CDS |
| GAGGTGGAGGGATATCGGTG+AGG | 0.544709 | 8_2:-36449877 | None:intergenic |
| AGGTGGAGGGATATCGGTGA+GGG | 0.546572 | 8_2:-36449876 | None:intergenic |
| GTGCTGCCTGCAAATTCTTG+AGG | 0.549706 | 8_2:+36449567 | Msa1246080:CDS |
| ACAGAAAGAGAGGCTGGAGG+AGG | 0.555018 | 8_2:-36449896 | None:intergenic |
| CGAACTCCTCCCTCACCAAA+GGG | 0.555403 | 8_2:+36449703 | Msa1246080:CDS |
| ACTGCATCCTCCCTTTGGTG+AGG | 0.557366 | 8_2:-36449713 | None:intergenic |
| TTGTCTTTGTAGAAAAGAGA+TGG | 0.561057 | 8_2:-36449798 | None:intergenic |
| GAGGGTATAGCGAAGAAGAT+CGG | 0.563089 | 8_2:-36449858 | None:intergenic |
| AAGAATTTGCAGGCAGCACA+AGG | 0.573114 | 8_2:-36449563 | None:intergenic |
| ACGAACTCCTCCCTCACCAA+AGG | 0.580542 | 8_2:+36449702 | Msa1246080:CDS |
| GATCCAGTTTATGGATGTGT+AGG | 0.582057 | 8_2:+36449773 | Msa1246080:CDS |
| TCCTTGAGGCACAGAAAGAG+AGG | 0.595065 | 8_2:-36449906 | None:intergenic |
| TCCTCAGTGGATATGAGTGA+AGG | 0.615767 | 8_2:+36450049 | Msa1246080:CDS |
| TAAGGTGCAAAGATGCAACC+TGG | 0.617157 | 8_2:-36449602 | None:intergenic |
| TCAGTGGATATGAGTGAAGG+TGG | 0.618537 | 8_2:+36450052 | Msa1246080:CDS |
| GTGGATATGAGTGAAGGTGG+AGG | 0.633891 | 8_2:+36450055 | Msa1246080:CDS |
| ACTGAGGAAGTATCAGTCCA+AGG | 0.638918 | 8_2:-36450034 | None:intergenic |
| TTGGACTGATACTTCCTCAG+TGG | 0.639081 | 8_2:+36450036 | Msa1246080:CDS |
| AGTTTAGTGGAAGAAATGAA+GGG | 0.640159 | 8_2:+36449960 | Msa1246080:CDS |
| ACAAGGTGAATTGTATGCGC+TGG | 0.642277 | 8_2:-36449546 | None:intergenic |
| TGGACTGATATCATTCCTTG+AGG | 0.655168 | 8_2:-36449920 | None:intergenic |
| ACTCCTCCCTCACCAAAGGG+AGG | 0.660217 | 8_2:+36449706 | Msa1246080:CDS |
| GGCACAGAAAGAGAGGCTGG+AGG | 0.697106 | 8_2:-36449899 | None:intergenic |
| ACCTTCACTCATATCCACTG+AGG | 0.758906 | 8_2:-36450050 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGAAGAATAAGTAGTAGT+AGG | - | chr8_2:36450001-36450020 | None:intergenic | 25.0% |
| ! | TGTACGTTTGCAAATTTTTG+AGG | - | chr8_2:36449644-36449663 | None:intergenic | 30.0% |
| GCAAACGTACACAAAATATT+TGG | + | chr8_2:36449653-36449672 | Msa1246080:CDS | 30.0% | |
| TTGTCTTTGTAGAAAAGAGA+TGG | - | chr8_2:36449801-36449820 | None:intergenic | 30.0% | |
| TTCTACAAAGACAAGTTCAA+AGG | + | chr8_2:36449807-36449826 | Msa1246080:CDS | 30.0% | |
| AGTTTAGTGGAAGAAATGAA+GGG | + | chr8_2:36449960-36449979 | Msa1246080:CDS | 30.0% | |
| ! | TTTTCTCCTCAAGAATTTGC+AGG | - | chr8_2:36449576-36449595 | None:intergenic | 35.0% |
| ACAAGTTCAAAGGCTTCAAA+AGG | + | chr8_2:36449817-36449836 | Msa1246080:CDS | 35.0% | |
| CTAAACTGTCTTTGTGGAAT+TGG | - | chr8_2:36449947-36449966 | None:intergenic | 35.0% | |
| ATTCCACAAAGACAGTTTAG+TGG | + | chr8_2:36449947-36449966 | Msa1246080:CDS | 35.0% | |
| CAGTTTAGTGGAAGAAATGA+AGG | + | chr8_2:36449959-36449978 | Msa1246080:CDS | 35.0% | |
| CTTTACCTTATTCTCTTCCT+TGG | + | chr8_2:36450017-36450036 | Msa1246080:CDS | 35.0% | |
| !!! | ATTTTTGAGGCTCTTCTGGA+GGG | - | chr8_2:36449631-36449650 | None:intergenic | 40.0% |
| !!! | AATTTTTGAGGCTCTTCTGG+AGG | - | chr8_2:36449632-36449651 | None:intergenic | 40.0% |
| !!! | GCAAATTTTTGAGGCTCTTC+TGG | - | chr8_2:36449635-36449654 | None:intergenic | 40.0% |
| GATCCAGTTTATGGATGTGT+AGG | + | chr8_2:36449773-36449792 | Msa1246080:CDS | 40.0% | |
| TGGACTGATATCATTCCTTG+AGG | - | chr8_2:36449923-36449942 | None:intergenic | 40.0% | |
| ACTGTCTTTGTGGAATTGGT+TGG | - | chr8_2:36449943-36449962 | None:intergenic | 40.0% | |
| CTTCCACTAAACTGTCTTTG+TGG | - | chr8_2:36449953-36449972 | None:intergenic | 40.0% | |
| TCAGTCCAAGGAAGAGAATA+AGG | - | chr8_2:36450025-36450044 | None:intergenic | 40.0% | |
| ACAAGGTGAATTGTATGCGC+TGG | - | chr8_2:36449549-36449568 | None:intergenic | 45.0% | |
| AAGAATTTGCAGGCAGCACA+AGG | - | chr8_2:36449566-36449585 | None:intergenic | 45.0% | |
| TTGAGGAGAAAATGCATGCC+AGG | + | chr8_2:36449584-36449603 | Msa1246080:CDS | 45.0% | |
| TAAGGTGCAAAGATGCAACC+TGG | - | chr8_2:36449605-36449624 | None:intergenic | 45.0% | |
| AGTTAACTGCATCCTCCCTT+TGG | - | chr8_2:36449721-36449740 | None:intergenic | 45.0% | |
| CGAGTTAGGGATCCAGTTTA+TGG | + | chr8_2:36449764-36449783 | Msa1246080:CDS | 45.0% | |
| GCTCCTACACATCCATAAAC+TGG | - | chr8_2:36449779-36449798 | None:intergenic | 45.0% | |
| ! | GAGGGTATAGCGAAGAAGAT+CGG | - | chr8_2:36449861-36449880 | None:intergenic | 45.0% |
| !! | ACTGAGGAAGTATCAGTCCA+AGG | - | chr8_2:36450037-36450056 | None:intergenic | 45.0% |
| TTGGACTGATACTTCCTCAG+TGG | + | chr8_2:36450036-36450055 | Msa1246080:CDS | 45.0% | |
| TCCTCAGTGGATATGAGTGA+AGG | + | chr8_2:36450049-36450068 | Msa1246080:CDS | 45.0% | |
| ACCTTCACTCATATCCACTG+AGG | - | chr8_2:36450053-36450072 | None:intergenic | 45.0% | |
| TCAGTGGATATGAGTGAAGG+TGG | + | chr8_2:36450052-36450071 | Msa1246080:CDS | 45.0% | |
| GTGCTGCCTGCAAATTCTTG+AGG | + | chr8_2:36449567-36449586 | Msa1246080:CDS | 50.0% | |
| TGGATCCCTAACTCGTGCTT+CGG | - | chr8_2:36449759-36449778 | None:intergenic | 50.0% | |
| TCCTTGAGGCACAGAAAGAG+AGG | - | chr8_2:36449909-36449928 | None:intergenic | 50.0% | |
| GTGGATATGAGTGAAGGTGG+AGG | + | chr8_2:36450055-36450074 | Msa1246080:CDS | 50.0% | |
| GATATGAGTGAAGGTGGAGG+AGG | + | chr8_2:36450058-36450077 | Msa1246080:CDS | 50.0% | |
| ! | GGCTCTTCTGGAGGGAAGTA+AGG | - | chr8_2:36449623-36449642 | None:intergenic | 55.0% |
| ACGAACTCCTCCCTCACCAA+AGG | + | chr8_2:36449702-36449721 | Msa1246080:CDS | 55.0% | |
| CGAACTCCTCCCTCACCAAA+GGG | + | chr8_2:36449703-36449722 | Msa1246080:CDS | 55.0% | |
| CTGCATCCTCCCTTTGGTGA+GGG | - | chr8_2:36449715-36449734 | None:intergenic | 55.0% | |
| ACTGCATCCTCCCTTTGGTG+AGG | - | chr8_2:36449716-36449735 | None:intergenic | 55.0% | |
| ACGAAGCCGAAGCACGAGTT+AGG | + | chr8_2:36449750-36449769 | Msa1246080:CDS | 55.0% | |
| CGAAGCCGAAGCACGAGTTA+GGG | + | chr8_2:36449751-36449770 | Msa1246080:CDS | 55.0% | |
| AGGTGGAGGGATATCGGTGA+GGG | - | chr8_2:36449879-36449898 | None:intergenic | 55.0% | |
| TGGAGGAGGTGGAGGGATAT+CGG | - | chr8_2:36449885-36449904 | None:intergenic | 55.0% | |
| ACAGAAAGAGAGGCTGGAGG+AGG | - | chr8_2:36449899-36449918 | None:intergenic | 55.0% | |
| TGAGGCACAGAAAGAGAGGC+TGG | - | chr8_2:36449905-36449924 | None:intergenic | 55.0% | |
| GCCTCTCTTTCTGTGCCTCA+AGG | + | chr8_2:36449905-36449924 | Msa1246080:CDS | 55.0% | |
| ATGAGTGAAGGTGGAGGAGG+AGG | + | chr8_2:36450061-36450080 | Msa1246080:CDS | 55.0% | |
| ACTCCTCCCTCACCAAAGGG+AGG | + | chr8_2:36449706-36449725 | Msa1246080:CDS | 60.0% | |
| CATCCTCCCTTTGGTGAGGG+AGG | - | chr8_2:36449712-36449731 | None:intergenic | 60.0% | |
| GAGGTGGAGGGATATCGGTG+AGG | - | chr8_2:36449880-36449899 | None:intergenic | 60.0% | |
| GAAAGAGAGGCTGGAGGAGG+TGG | - | chr8_2:36449896-36449915 | None:intergenic | 60.0% | |
| GGCACAGAAAGAGAGGCTGG+AGG | - | chr8_2:36449902-36449921 | None:intergenic | 60.0% | |
| AGTGAAGGTGGAGGAGGAGG+AGG | + | chr8_2:36450064-36450083 | Msa1246080:CDS | 60.0% | |
| GAGAGGCTGGAGGAGGTGGA+GGG | - | chr8_2:36449892-36449911 | None:intergenic | 65.0% | |
| AGAGAGGCTGGAGGAGGTGG+AGG | - | chr8_2:36449893-36449912 | None:intergenic | 65.0% | |
| GAAGGTGGAGGAGGAGGAGG+AGG | + | chr8_2:36450067-36450086 | Msa1246080:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 36449533 | 36450102 | 36449533 | ID=Msa1246080;Name=Msa1246080 |
| chr8_2 | mRNA | 36449533 | 36450102 | 36449533 | ID=Msa1246080-mRNA-1;Parent=Msa1246080;Name=Msa1246080-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0|-1|0|1|-1|1|1|0|189 |
| chr8_2 | exon | 36449533 | 36450102 | 36449533 | ID=Msa1246080-mRNA-1:exon:11665;Parent=Msa1246080-mRNA-1 |
| chr8_2 | CDS | 36449533 | 36450102 | 36449533 | ID=Msa1246080-mRNA-1:cds;Parent=Msa1246080-mRNA-1 |
| Gene Sequence |
| Protein sequence |
