AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1330510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1330510 MtrunA17_Chr4g0058651 95.789 190 6 1 1 190 1 188 1.71e-126 353
Msa1330510 MtrunA17_Chr3g0117121 73.743 179 44 3 1 178 1 177 1.10e-85 250
Msa1330510 MtrunA17_Chr7g0227511 62.234 188 54 4 1 188 1 171 7.98e-73 217
Msa1330510 MtrunA17_Chr6g0449641 82.301 113 18 1 1 113 1 111 2.40e-65 197
Msa1330510 MtrunA17_Chr6g0449651 79.130 115 22 1 1 115 59 171 2.15e-63 194
Msa1330510 MtrunA17_Chr8g0353981 66.917 133 40 1 1 133 1 129 1.03e-53 171
Msa1330510 MtrunA17_Chr5g0437571 64.957 117 38 1 9 122 4 120 1.42e-50 160
Msa1330510 MtrunA17_Chr5g0402611 69.091 110 34 0 5 114 2 111 3.84e-50 164
Msa1330510 MtrunA17_Chr3g0115431 64.754 122 43 0 5 126 3 124 4.74e-50 161
Msa1330510 MtrunA17_Chr3g0097221 65.179 112 39 0 6 117 2 113 4.06e-48 158
Msa1330510 MtrunA17_Chr2g0311601 63.551 107 39 0 10 116 12 118 8.84e-48 153
Msa1330510 MtrunA17_Chr4g0043421 65.385 104 36 0 10 113 14 117 2.74e-47 152
Msa1330510 MtrunA17_Chr3g0106101 64.423 104 37 0 9 112 5 108 6.30e-47 152
Msa1330510 MtrunA17_Chr5g0437401 53.383 133 62 0 9 141 9 141 4.66e-46 149
Msa1330510 MtrunA17_Chr3g0114061 66.019 103 35 0 10 112 7 109 6.89e-46 148
Msa1330510 MtrunA17_Chr5g0438091 62.385 109 41 0 10 118 38 146 6.88e-45 147
Msa1330510 MtrunA17_Chr3g0129071 61.468 109 42 0 10 118 46 154 1.64e-44 147
Msa1330510 MtrunA17_Chr3g0113891 63.000 100 37 0 9 108 12 111 2.02e-42 140
Msa1330510 MtrunA17_Chr5g0432451 58.015 131 54 1 10 140 42 171 4.79e-40 135
Msa1330510 MtrunA17_Chr4g0030001 53.448 116 53 1 2 116 15 130 6.68e-40 134
Msa1330510 MtrunA17_Chr7g0260971 59.130 115 43 2 1 111 1 115 3.69e-39 133
Msa1330510 MtrunA17_Chr1g0200201 58.416 101 42 0 12 112 73 173 1.20e-38 132
Msa1330510 MtrunA17_Chr8g0351701 57.944 107 44 1 11 116 23 129 1.59e-38 132
Msa1330510 MtrunA17_Chr8g0351721 54.918 122 52 2 11 131 18 137 4.32e-36 125
Msa1330510 MtrunA17_Chr1g0184281 62.105 95 35 1 11 104 15 109 7.63e-36 124
Msa1330510 MtrunA17_Chr7g0225111 51.376 109 53 0 10 118 4 112 1.08e-35 121
Msa1330510 MtrunA17_Chr7g0245871 62.105 95 35 1 11 104 27 121 3.03e-35 124
Msa1330510 MtrunA17_Chr1g0184271 37.572 173 98 3 11 177 12 180 3.69e-34 120
Msa1330510 MtrunA17_Chr6g0460951 51.852 108 51 1 10 116 39 146 5.14e-34 120
Msa1330510 MtrunA17_Chr5g0435641 52.475 101 47 1 6 105 2 102 4.70e-33 117
Msa1330510 MtrunA17_Chr8g0374601 48.077 104 54 0 11 114 26 129 1.06e-32 118
Msa1330510 MtrunA17_Chr7g0261031 58.333 96 39 1 10 104 7 102 1.08e-32 117
Msa1330510 MtrunA17_Chr5g0444351 48.598 107 55 0 12 118 6 112 5.64e-32 112
Msa1330510 MtrunA17_Chr8g0374591 48.077 104 54 0 11 114 8 111 1.84e-31 110
Msa1330510 MtrunA17_Chr7g0245861 60.000 90 33 2 11 97 12 101 1.51e-29 106
Msa1330510 MtrunA17_Chr4g0059901 48.000 100 52 0 12 111 11 110 2.05e-29 109
Msa1330510 MtrunA17_Chr8g0374581 46.667 105 55 1 11 114 8 112 2.12e-28 105
Msa1330510 MtrunA17_Chr6g0452661 44.898 98 53 1 9 105 7 104 7.18e-28 105
Msa1330510 MtrunA17_Chr6g0460941 48.315 89 45 1 11 98 7 95 1.96e-26 97.1
Msa1330510 MtrunA17_Chr8g0342351 43.000 100 57 0 12 111 13 112 5.44e-26 100
Msa1330510 MtrunA17_Chr6g0452671 36.220 127 72 2 12 130 12 137 1.12e-24 95.5
Msa1330510 MtrunA17_Chr6g0452691 49.398 83 41 1 9 90 7 89 1.75e-24 94.7
Msa1330510 MtrunA17_Chr4g0055441 40.385 104 60 2 12 114 3 105 9.35e-24 92.0
Msa1330510 MtrunA17_Chr2g0330201 43.011 93 53 0 12 104 20 112 4.59e-23 93.6
Msa1330510 MtrunA17_Chr4g0040831 39.423 104 63 0 1 104 5 108 9.64e-23 90.5
Msa1330510 MtrunA17_Chr5g0439111 40.187 107 62 2 9 114 7 112 1.85e-20 85.1
Msa1330510 MtrunA17_Chr2g0326041 60.784 51 20 0 10 60 32 82 5.75e-19 77.4
Msa1330510 MtrunA17_Chr4g0046201 35.556 90 58 0 10 99 5 94 4.75e-17 76.6
Msa1330510 MtrunA17_Chr2g0326051 54.098 61 28 0 56 116 5 65 6.54e-16 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1330510 AT5G63090.4 68.333 180 45 3 1 171 1 177 2.40e-78 232
Msa1330510 AT5G63090.3 68.333 180 45 3 1 171 1 177 2.40e-78 232
Msa1330510 AT5G63090.2 68.333 180 45 3 1 171 1 177 2.40e-78 232
Msa1330510 AT5G63090.1 68.333 180 45 3 1 171 1 177 2.40e-78 232
Msa1330510 AT3G27650.1 78.182 110 24 0 5 114 33 142 3.06e-62 190
Msa1330510 AT1G65620.1 68.217 129 39 1 1 129 1 127 1.65e-52 167
Msa1330510 AT1G65620.4 68.217 129 39 1 1 129 1 127 1.65e-52 167
Msa1330510 AT1G65620.3 68.217 129 39 1 1 129 1 127 1.65e-52 167
Msa1330510 AT1G65620.5 68.217 129 39 1 1 129 1 127 1.65e-52 167
Msa1330510 AT1G65620.2 68.217 129 39 1 1 129 1 127 1.65e-52 167
Msa1330510 AT5G66870.1 60.839 143 45 3 1 142 1 133 7.56e-51 166
Msa1330510 AT2G30130.1 64.286 112 40 0 9 120 6 117 2.92e-49 158
Msa1330510 AT2G23660.2 62.931 116 43 0 9 124 3 118 3.08e-49 162
Msa1330510 AT2G23660.3 62.931 116 43 0 9 124 3 118 3.08e-49 162
Msa1330510 AT2G23660.1 62.931 116 43 0 9 124 3 118 3.08e-49 162
Msa1330510 AT1G31320.1 72.727 88 24 0 10 97 12 99 3.76e-44 145
Msa1330510 AT1G16530.1 59.829 117 45 2 10 124 13 129 1.72e-43 143
Msa1330510 AT1G07900.1 61.165 103 40 0 10 112 32 134 5.04e-43 142
Msa1330510 AT2G30340.2 60.952 105 41 0 10 114 52 156 1.35e-42 144
Msa1330510 AT2G30340.1 60.952 105 41 0 10 114 51 155 1.41e-42 144
Msa1330510 AT2G28500.1 60.194 103 41 0 10 112 54 156 2.70e-41 139
Msa1330510 AT3G11090.1 57.944 107 45 0 9 115 9 115 5.05e-41 136
Msa1330510 AT2G40470.2 63.810 105 38 0 10 114 19 123 8.07e-39 132
Msa1330510 AT2G40470.1 63.810 105 38 0 10 114 44 148 1.86e-38 132
Msa1330510 AT3G26660.1 55.000 100 45 0 12 111 6 105 1.08e-37 127
Msa1330510 AT3G26620.1 55.000 100 45 0 12 111 6 105 1.17e-37 126
Msa1330510 AT3G03760.1 56.731 104 44 1 10 112 50 153 2.15e-37 130
Msa1330510 AT4G00210.1 54.472 123 53 2 1 122 1 121 4.43e-37 128
Msa1330510 AT2G45420.1 63.043 92 33 1 14 104 40 131 1.43e-35 125
Msa1330510 AT4G00220.1 57.692 104 43 1 14 116 20 123 1.86e-35 124
Msa1330510 AT2G31310.1 38.503 187 84 5 10 170 6 187 6.21e-35 122
Msa1330510 AT2G42430.1 55.963 109 43 2 1 104 1 109 2.12e-34 122
Msa1330510 AT3G50510.1 45.902 122 64 1 10 129 11 132 9.42e-34 119
Msa1330510 AT3G50510.2 45.902 122 64 1 10 129 11 132 9.42e-34 119
Msa1330510 AT3G58190.1 56.250 96 41 1 11 105 11 106 3.33e-33 118
Msa1330510 AT2G45410.1 52.542 118 53 2 14 130 19 134 4.88e-33 117
Msa1330510 AT2G42440.1 53.608 97 44 1 10 105 6 102 2.16e-32 117
Msa1330510 AT5G06080.1 47.368 114 59 1 6 118 2 115 4.27e-32 114
Msa1330510 AT5G35900.1 49.000 100 51 0 12 111 6 105 1.11e-30 111
Msa1330510 AT3G13850.1 45.745 94 51 0 12 105 37 130 5.43e-28 106
Msa1330510 AT3G47870.1 41.748 103 60 0 12 114 37 139 4.43e-25 99.8
Msa1330510 AT1G72980.1 41.905 105 61 0 1 105 3 107 1.42e-23 93.2
Msa1330510 AT4G22700.1 39.640 111 65 2 12 121 6 115 6.44e-21 85.9
Msa1330510 AT1G06280.1 44.156 77 43 0 12 88 25 101 7.50e-18 78.2
Msa1330510 AT2G19820.1 34.694 98 63 1 2 98 3 100 3.20e-15 69.3
Msa1330510 AT5G15060.1 40.260 77 44 1 8 82 14 90 4.42e-15 69.3
Msa1330510 AT3G27940.3 38.806 67 41 0 10 76 4 70 1.13e-11 60.5
Msa1330510 AT3G27940.1 38.806 67 41 0 10 76 4 70 1.13e-11 60.5
Msa1330510 AT3G27940.2 38.806 67 41 0 10 76 4 70 1.13e-11 60.5

Find 55 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAACGTACACAAAATATT+TGG 0.186655 8_4:-49693857 Msa1330510:CDS
CTAAACTGTCTTTGTGGAAT+TGG 0.275981 8_4:+49693566 None:intergenic
CGAGTTAGGGATCCAGTTTA+TGG 0.289758 8_4:-49693746 Msa1330510:CDS
AGAGAGGCTGGAGGAGGTGG+AGG 0.297872 8_4:+49693620 None:intergenic
ACGAAGCCGAAGCACGAGTT+AGG 0.305462 8_4:-49693760 Msa1330510:CDS
CTTTACCTTATTCTCTTCCT+TGG 0.333470 8_4:-49693493 Msa1330510:CDS
ATGAGTGAAGGTGGAGGAGG+AGG 0.339514 8_4:-49693449 Msa1330510:CDS
TCAGTCCAAGGAAGAGAATA+AGG 0.339719 8_4:+49693488 None:intergenic
AGTGAAGGTGGAGGAGGAGG+AGG 0.357862 8_4:-49693446 Msa1330510:CDS
GAAGGTGGAGGAGGAGGAGG+AGG 0.363314 8_4:-49693443 Msa1330510:CDS
AGTTAACTGCATCCTCCCTT+TGG 0.396415 8_4:+49693792 None:intergenic
AAAGAAGAATAAGTAGTAGT+AGG 0.401220 8_4:+49693512 None:intergenic
GCTCCTACACATCCATAAAC+TGG 0.405024 8_4:+49693734 None:intergenic
GAAAGAGAGGCTGGAGGAGG+TGG 0.409459 8_4:+49693617 None:intergenic
CTTCCACTAAACTGTCTTTG+TGG 0.427794 8_4:+49693560 None:intergenic
GGCTCTTCTGGAGGGAAGTA+AGG 0.432439 8_4:+49693890 None:intergenic
TGGAGGAGGTGGAGGGATAT+CGG 0.435988 8_4:+49693628 None:intergenic
GATATGAGTGAAGGTGGAGG+AGG 0.442790 8_4:-49693452 Msa1330510:CDS
ATTCCACAAAGACAGTTTAG+TGG 0.446210 8_4:-49693563 Msa1330510:CDS
TGGATCCCTAACTCGTGCTT+CGG 0.456667 8_4:+49693754 None:intergenic
CTGCATCCTCCCTTTGGTGC+GGG 0.467494 8_4:+49693798 None:intergenic
CAGTTTAGTGGAAGAAATGA+AGG 0.473637 8_4:-49693551 Msa1330510:CDS
TGAGGCACAGAAAGAGAGGC+TGG 0.482119 8_4:+49693608 None:intergenic
ACTGTCTTTGTGGAATTGGT+TGG 0.483148 8_4:+49693570 None:intergenic
TTGAGGAGAAAATGCATGCC+AGG 0.496470 8_4:-49693926 Msa1330510:CDS
ACAAGTTCAAAGGCTTCAAA+AGG 0.500774 8_4:-49693693 Msa1330510:CDS
GCCTCTCTTTCTGTGCCTCA+AGG 0.505853 8_4:-49693605 Msa1330510:CDS
CGAAGCCGAAGCACGAGTTA+GGG 0.512333 8_4:-49693759 Msa1330510:CDS
GAGAGGCTGGAGGAGGTGGA+GGG 0.516068 8_4:+49693621 None:intergenic
TTCTACAAAGACAAGTTCAA+AGG 0.528529 8_4:-49693703 Msa1330510:CDS
ACGAACTCCTCCCGCACCAA+AGG 0.531966 8_4:-49693808 Msa1330510:CDS
CATCCTCCCTTTGGTGCGGG+AGG 0.533656 8_4:+49693801 None:intergenic
GAGGTGGAGGGATATCGGTG+AGG 0.544709 8_4:+49693633 None:intergenic
AGGTGGAGGGATATCGGTGA+GGG 0.546572 8_4:+49693634 None:intergenic
CGAACTCCTCCCGCACCAAA+GGG 0.546891 8_4:-49693807 Msa1330510:CDS
GTGCTGCCTGCAAATTCTTG+AGG 0.549706 8_4:-49693943 Msa1330510:CDS
ACAGAAAGAGAGGCTGGAGG+AGG 0.555018 8_4:+49693614 None:intergenic
TTGTCTTTGTAGAAAAGAGA+TGG 0.561057 8_4:+49693712 None:intergenic
GAGGGTATAGCGAAGAAGAT+CGG 0.563089 8_4:+49693652 None:intergenic
AAGAATTTGCAGGCAGCACA+AGG 0.573114 8_4:+49693947 None:intergenic
ACTGCATCCTCCCTTTGGTG+CGG 0.573325 8_4:+49693797 None:intergenic
GATCCAGTTTATGGATGTGT+AGG 0.582057 8_4:-49693737 Msa1330510:CDS
TCCTTGAGGCACAGAAAGAG+AGG 0.595065 8_4:+49693604 None:intergenic
TCCTCAGTGGATATGAGTGA+AGG 0.615767 8_4:-49693461 Msa1330510:CDS
TAAGGTGCAAAGATGCAACC+TGG 0.617157 8_4:+49693908 None:intergenic
TCAGTGGATATGAGTGAAGG+TGG 0.618537 8_4:-49693458 Msa1330510:CDS
GTGGATATGAGTGAAGGTGG+AGG 0.633891 8_4:-49693455 Msa1330510:CDS
ACTGAGGAAGTATCAGTCCA+AGG 0.638918 8_4:+49693476 None:intergenic
TTGGACTGATACTTCCTCAG+TGG 0.639081 8_4:-49693474 Msa1330510:CDS
AGTTTAGTGGAAGAAATGAA+GGG 0.640159 8_4:-49693550 Msa1330510:CDS
ACAAGGTGAATTGTATGCGC+TGG 0.642277 8_4:+49693964 None:intergenic
ACTCCTCCCGCACCAAAGGG+AGG 0.644323 8_4:-49693804 Msa1330510:CDS
TGGACTGATATCATTCCTTG+AGG 0.655168 8_4:+49693590 None:intergenic
GGCACAGAAAGAGAGGCTGG+AGG 0.697106 8_4:+49693611 None:intergenic
ACCTTCACTCATATCCACTG+AGG 0.758906 8_4:+49693460 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAGAAGAATAAGTAGTAGT+AGG + chr8_4:49693895-49693914 None:intergenic 25.0%
! TGTACGTTTGCAAATTTTTG+AGG + chr8_4:49693538-49693557 None:intergenic 30.0%
GCAAACGTACACAAAATATT+TGG - chr8_4:49693547-49693566 Msa1330510:CDS 30.0%
TTGTCTTTGTAGAAAAGAGA+TGG + chr8_4:49693695-49693714 None:intergenic 30.0%
TTCTACAAAGACAAGTTCAA+AGG - chr8_4:49693701-49693720 Msa1330510:CDS 30.0%
AGTTTAGTGGAAGAAATGAA+GGG - chr8_4:49693854-49693873 Msa1330510:CDS 30.0%
! TTTTCTCCTCAAGAATTTGC+AGG + chr8_4:49693470-49693489 None:intergenic 35.0%
ACAAGTTCAAAGGCTTCAAA+AGG - chr8_4:49693711-49693730 Msa1330510:CDS 35.0%
CTAAACTGTCTTTGTGGAAT+TGG + chr8_4:49693841-49693860 None:intergenic 35.0%
ATTCCACAAAGACAGTTTAG+TGG - chr8_4:49693841-49693860 Msa1330510:CDS 35.0%
CAGTTTAGTGGAAGAAATGA+AGG - chr8_4:49693853-49693872 Msa1330510:CDS 35.0%
CTTTACCTTATTCTCTTCCT+TGG - chr8_4:49693911-49693930 Msa1330510:CDS 35.0%
!!! ATTTTTGAGGCTCTTCTGGA+GGG + chr8_4:49693525-49693544 None:intergenic 40.0%
!!! AATTTTTGAGGCTCTTCTGG+AGG + chr8_4:49693526-49693545 None:intergenic 40.0%
!!! GCAAATTTTTGAGGCTCTTC+TGG + chr8_4:49693529-49693548 None:intergenic 40.0%
GATCCAGTTTATGGATGTGT+AGG - chr8_4:49693667-49693686 Msa1330510:CDS 40.0%
TGGACTGATATCATTCCTTG+AGG + chr8_4:49693817-49693836 None:intergenic 40.0%
ACTGTCTTTGTGGAATTGGT+TGG + chr8_4:49693837-49693856 None:intergenic 40.0%
CTTCCACTAAACTGTCTTTG+TGG + chr8_4:49693847-49693866 None:intergenic 40.0%
TCAGTCCAAGGAAGAGAATA+AGG + chr8_4:49693919-49693938 None:intergenic 40.0%
ACAAGGTGAATTGTATGCGC+TGG + chr8_4:49693443-49693462 None:intergenic 45.0%
AAGAATTTGCAGGCAGCACA+AGG + chr8_4:49693460-49693479 None:intergenic 45.0%
TTGAGGAGAAAATGCATGCC+AGG - chr8_4:49693478-49693497 Msa1330510:CDS 45.0%
TAAGGTGCAAAGATGCAACC+TGG + chr8_4:49693499-49693518 None:intergenic 45.0%
AGTTAACTGCATCCTCCCTT+TGG + chr8_4:49693615-49693634 None:intergenic 45.0%
CGAGTTAGGGATCCAGTTTA+TGG - chr8_4:49693658-49693677 Msa1330510:CDS 45.0%
GCTCCTACACATCCATAAAC+TGG + chr8_4:49693673-49693692 None:intergenic 45.0%
! GAGGGTATAGCGAAGAAGAT+CGG + chr8_4:49693755-49693774 None:intergenic 45.0%
!! ACTGAGGAAGTATCAGTCCA+AGG + chr8_4:49693931-49693950 None:intergenic 45.0%
TTGGACTGATACTTCCTCAG+TGG - chr8_4:49693930-49693949 Msa1330510:CDS 45.0%
TCCTCAGTGGATATGAGTGA+AGG - chr8_4:49693943-49693962 Msa1330510:CDS 45.0%
ACCTTCACTCATATCCACTG+AGG + chr8_4:49693947-49693966 None:intergenic 45.0%
TCAGTGGATATGAGTGAAGG+TGG - chr8_4:49693946-49693965 Msa1330510:CDS 45.0%
GTGCTGCCTGCAAATTCTTG+AGG - chr8_4:49693461-49693480 Msa1330510:CDS 50.0%
TGGATCCCTAACTCGTGCTT+CGG + chr8_4:49693653-49693672 None:intergenic 50.0%
TCCTTGAGGCACAGAAAGAG+AGG + chr8_4:49693803-49693822 None:intergenic 50.0%
GTGGATATGAGTGAAGGTGG+AGG - chr8_4:49693949-49693968 Msa1330510:CDS 50.0%
GATATGAGTGAAGGTGGAGG+AGG - chr8_4:49693952-49693971 Msa1330510:CDS 50.0%
! GGCTCTTCTGGAGGGAAGTA+AGG + chr8_4:49693517-49693536 None:intergenic 55.0%
ACTGCATCCTCCCTTTGGTG+CGG + chr8_4:49693610-49693629 None:intergenic 55.0%
ACGAAGCCGAAGCACGAGTT+AGG - chr8_4:49693644-49693663 Msa1330510:CDS 55.0%
CGAAGCCGAAGCACGAGTTA+GGG - chr8_4:49693645-49693664 Msa1330510:CDS 55.0%
AGGTGGAGGGATATCGGTGA+GGG + chr8_4:49693773-49693792 None:intergenic 55.0%
TGGAGGAGGTGGAGGGATAT+CGG + chr8_4:49693779-49693798 None:intergenic 55.0%
ACAGAAAGAGAGGCTGGAGG+AGG + chr8_4:49693793-49693812 None:intergenic 55.0%
TGAGGCACAGAAAGAGAGGC+TGG + chr8_4:49693799-49693818 None:intergenic 55.0%
GCCTCTCTTTCTGTGCCTCA+AGG - chr8_4:49693799-49693818 Msa1330510:CDS 55.0%
ATGAGTGAAGGTGGAGGAGG+AGG - chr8_4:49693955-49693974 Msa1330510:CDS 55.0%
ACGAACTCCTCCCGCACCAA+AGG - chr8_4:49693596-49693615 Msa1330510:CDS 60.0%
CGAACTCCTCCCGCACCAAA+GGG - chr8_4:49693597-49693616 Msa1330510:CDS 60.0%
! CTGCATCCTCCCTTTGGTGC+GGG + chr8_4:49693609-49693628 None:intergenic 60.0%
GAGGTGGAGGGATATCGGTG+AGG + chr8_4:49693774-49693793 None:intergenic 60.0%
GAAAGAGAGGCTGGAGGAGG+TGG + chr8_4:49693790-49693809 None:intergenic 60.0%
GGCACAGAAAGAGAGGCTGG+AGG + chr8_4:49693796-49693815 None:intergenic 60.0%
AGTGAAGGTGGAGGAGGAGG+AGG - chr8_4:49693958-49693977 Msa1330510:CDS 60.0%
ACTCCTCCCGCACCAAAGGG+AGG - chr8_4:49693600-49693619 Msa1330510:CDS 65.0%
! CATCCTCCCTTTGGTGCGGG+AGG + chr8_4:49693606-49693625 None:intergenic 65.0%
GAGAGGCTGGAGGAGGTGGA+GGG + chr8_4:49693786-49693805 None:intergenic 65.0%
AGAGAGGCTGGAGGAGGTGG+AGG + chr8_4:49693787-49693806 None:intergenic 65.0%
GAAGGTGGAGGAGGAGGAGG+AGG - chr8_4:49693961-49693980 Msa1330510:CDS 65.0%
GGTGGAGGAGGAGGAGGAGG+AGG - chr8_4:49693964-49693983 Msa1330510:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 49693427 49693999 49693427 ID=Msa1330510;Name=Msa1330510
chr8_4 mRNA 49693427 49693999 49693427 ID=Msa1330510-mRNA-1;Parent=Msa1330510;Name=Msa1330510-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0|-1|0|1|-1|1|1|0|190
chr8_4 exon 49693427 49693999 49693427 ID=Msa1330510-mRNA-1:exon:14459;Parent=Msa1330510-mRNA-1
chr8_4 CDS 49693427 49693999 49693427 ID=Msa1330510-mRNA-1:cds;Parent=Msa1330510-mRNA-1
Gene Sequence

>Msa1330510

ATGGCATCATCATCCAGCGCATACAATTCACCTTGTGCTGCCTGCAAATTCTTGAGGAGAAAATGCATGCCAGGTTGCATCTTTGCACCTTACTTCCCTCCAGAAGAGCCTCAAAAATTTGCAAACGTACACAAAATATTTGGTGCTAGCAATGTCACAAAGCTTCTCAACGAACTCCTCCCGCACCAAAGGGAGGATGCAGTTAACTCACTTGCTTACGAAGCCGAAGCACGAGTTAGGGATCCAGTTTATGGATGTGTAGGAGCCATCTCTTTTCTACAAAGACAAGTTCAAAGGCTTCAAAAGGAACTCGACGCTGCTAACACCGATCTTCTTCGCTATACCCTCACCGATATCCCTCCACCTCCTCCAGCCTCTCTTTCTGTGCCTCAAGGAATGATATCAGTCCAACCAATTCCACAAAGACAGTTTAGTGGAAGAAATGAAGGGCTTTATAGACAATCTCCTACTACTACTTATTCTTCTTTACCTTATTCTCTTCCTTGGACTGATACTTCCTCAGTGGATATGAGTGAAGGTGGAGGAGGAGGAGGAGGAGGAAATCATATGTGA
Protein sequence

>Msa1330510

MASSSSAYNSPCAACKFLRRKCMPGCIFAPYFPPEEPQKFANVHKIFGASNVTKLLNELLPHQREDAVNSLAYEAEARVRDPVYGCVGAISFLQRQVQRLQKELDAANTDLLRYTLTDIPPPPPASLSVPQGMISVQPIPQRQFSGRNEGLYRQSPTTTYSSLPYSLPWTDTSSVDMSEGGGGGGGGNHM*