AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1210180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1210180 MtrunA17_Chr4g0059421 99.130 115 1 0 1 115 1 115 1.29e-80 231
Msa1210180 MtrunA17_Chr8g0364311 56.364 110 44 1 6 115 7 112 1.52e-38 125
Msa1210180 MtrunA17_Chr3g0142671 47.826 115 48 3 1 115 1 103 4.53e-28 98.6
Msa1210180 MtrunA17_Chr1g0156601 46.957 115 47 3 1 115 1 101 7.41e-27 95.5
Msa1210180 MtrunA17_Chr6g0451481 72.131 61 17 0 55 115 159 219 4.75e-25 94.4
Msa1210180 MtrunA17_Chr1g0156621 63.636 66 23 1 50 115 36 100 7.49e-23 85.1
Msa1210180 MtrunA17_Chr8g0371941 62.121 66 24 1 50 115 39 103 1.17e-21 82.4
Msa1210180 MtrunA17_Chr1g0200531 50.000 76 36 2 41 115 26 100 2.19e-19 76.6
Msa1210180 MtrunA17_Chr6g0451501 57.377 61 26 0 55 115 102 162 3.38e-17 72.4
Msa1210180 MtrunA17_Chr6g0451491 59.677 62 24 1 55 115 69 130 3.67e-17 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1210180 AT5G14920.1 66.129 62 21 0 54 115 214 275 2.04e-22 89.0
Msa1210180 AT4G09600.1 64.516 62 21 1 54 115 39 99 9.39e-22 82.8
Msa1210180 AT2G18420.1 62.903 62 22 1 54 115 34 94 8.00e-21 80.5
Msa1210180 AT2G18420.2 62.903 62 22 1 54 115 10 70 8.04e-21 79.7
Msa1210180 AT1G75750.1 57.576 66 27 1 50 115 34 98 1.09e-20 80.1
Msa1210180 AT1G75750.2 57.576 66 27 1 50 115 33 97 1.27e-20 79.7
Msa1210180 AT1G22690.1 58.065 62 25 1 54 115 59 119 6.00e-19 76.3
Msa1210180 AT1G22690.2 58.065 62 25 1 54 115 59 119 6.00e-19 76.3
Msa1210180 AT1G22690.3 58.065 62 25 1 54 115 59 119 6.00e-19 76.3
Msa1210180 AT4G09610.1 61.290 62 23 1 54 115 39 99 3.67e-13 60.8
Msa1210180 AT1G74670.1 31.858 113 65 2 3 115 1 101 2.44e-11 56.2

Find 46 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTTAATATTGTTGATATT+TGG 0.191398 8_1:-68947606 Msa1210180:three_prime_UTR
CATGCTCTCTTGCACAAATT+TGG 0.199596 8_1:+68947989 None:intergenic
TACTAGTGTAGTTTAGTAAA+TGG 0.245869 8_1:-68947786 Msa1210180:three_prime_UTR
GCCCATGGGCCTAATTAGTT+TGG 0.284816 8_1:-68947835 Msa1210180:three_prime_UTR
GGGCTTTGATAAGGTCTCTA+AGG 0.305310 8_1:+68947854 None:intergenic
TTACGCAAGCTTCTTGTCTT+CGG 0.310911 8_1:-68948664 Msa1210180:CDS
GAAGAGAGCCCAAACTAATT+AGG 0.320634 8_1:+68947826 None:intergenic
TACGCAAGCTTCTTGTCTTC+GGG 0.326759 8_1:-68948663 Msa1210180:CDS
CACCTGGTACTGCCGGTAAT+AGG 0.335185 8_1:-68947934 Msa1210180:CDS
TATGTTGTGGTAACTAACTA+TGG 0.368562 8_1:-68947659 Msa1210180:three_prime_UTR
ACCTGGTACTGCCGGTAATA+GGG 0.401958 8_1:-68947933 Msa1210180:CDS
CCCATGGGCCTAATTAGTTT+GGG 0.408002 8_1:-68947834 Msa1210180:three_prime_UTR
TCCACAAAACTCCCTATTAC+CGG 0.431014 8_1:+68947922 None:intergenic
TAGGCCCATGGGCTTTGATA+AGG 0.442762 8_1:+68947845 None:intergenic
CAAGGTGCAGTGTGCATTCA+AGG 0.442762 8_1:-68948012 Msa1210180:CDS
ACTAGTGTAGTTTAGTAAAT+GGG 0.467501 8_1:-68947785 Msa1210180:three_prime_UTR
CAGACTGTGGAGGGCTATGC+AGG 0.492139 8_1:-68948036 Msa1210180:CDS
ATTTACTAAACTACACTAGT+AGG 0.495839 8_1:+68947788 None:intergenic
CTTGGTCTTGTTACCATGAG+TGG 0.515862 8_1:+68947880 None:intergenic
ATCTGATCATGAGATAGAAA+AGG 0.540603 8_1:-68948353 Msa1210180:CDS
TCCCTATTACCGGCAGTACC+AGG 0.543014 8_1:+68947932 None:intergenic
AATGCATTTAACTGATAAGC+CGG 0.550151 8_1:-68948317 Msa1210180:intron
GCCCAAACTAATTAGGCCCA+TGG 0.551582 8_1:+68947833 None:intergenic
ATAAGGTCTCTAAGGGCACT+TGG 0.552905 8_1:+68947862 None:intergenic
TGTGTCCCACCTGGTACTGC+CGG 0.553087 8_1:-68947941 Msa1210180:CDS
GGCTTTGATAAGGTCTCTAA+GGG 0.558100 8_1:+68947855 None:intergenic
CTTCAGCTTATTGTGCGAGA+CGG 0.558872 8_1:-68948181 Msa1210180:intron
AGAGACCTTATCAAAGCCCA+TGG 0.565394 8_1:-68947850 Msa1210180:three_prime_UTR
TACACTGATATGACCACTCA+TGG 0.567522 8_1:-68947893 Msa1210180:CDS
TGTTAAACCTTTGTATTCGT+AGG 0.570058 8_1:-68947706 Msa1210180:three_prime_UTR
CCCAAACTAATTAGGCCCAT+GGG 0.581285 8_1:+68947834 None:intergenic
AGAAGGCTAATGCAAGACAT+AGG 0.585779 8_1:-68948154 Msa1210180:intron
AGGTGCAAGTGTGTCCCACC+TGG 0.590839 8_1:-68947950 Msa1210180:CDS
AATTTGTGCAAGAGAGCATG+TGG 0.594899 8_1:-68947986 Msa1210180:CDS
GTGGAGGGCTATGCAGGTCA+AGG 0.600120 8_1:-68948030 Msa1210180:CDS
TTGTGCGAGACGGAAACAGA+AGG 0.605905 8_1:-68948171 Msa1210180:CDS
TCAAAATCCTACGAATACAA+AGG 0.641840 8_1:+68947699 None:intergenic
CATGTGGGACTTGCTGTGTG+AGG 0.647718 8_1:-68947970 Msa1210180:CDS
TACAAGTTAGTACCTTAGAG+AGG 0.650629 8_1:+68948623 None:intergenic
CTTGCGTAATGCCATTAGAG+AGG 0.657310 8_1:+68948678 None:intergenic
GAGACCTTATCAAAGCCCAT+GGG 0.661282 8_1:-68947849 Msa1210180:three_prime_UTR
CTACACTAGTAGGTACTACG+TGG 0.678386 8_1:+68947798 None:intergenic
TACACTAGTAGGTACTACGT+GGG 0.679336 8_1:+68947799 None:intergenic
CTATTACCGGCAGTACCAGG+TGG 0.706350 8_1:+68947935 None:intergenic
TATTACCGGCAGTACCAGGT+GGG 0.719553 8_1:+68947936 None:intergenic
ATTTGTGCAAGAGAGCATGT+GGG 0.729137 8_1:-68947985 Msa1210180:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTATATTTAGTTTTATTTTT+TGG + chr8_1:68948192-68948211 None:intergenic 10.0%
!!! ACAAATTAAGATTTTTTATA+AGG - chr8_1:68948225-68948244 Msa1210180:intron 10.0%
!! TTTGAATGAAAAAAAATAAA+AGG - chr8_1:68948562-68948581 Msa1210180:intron 10.0%
!!! GTATATTTAGTTTTATTTTT+TGG + chr8_1:68948192-68948211 None:intergenic 10.0%
!!! ACAAATTAAGATTTTTTATA+AGG - chr8_1:68948225-68948244 Msa1210180:intron 10.0%
!! TTTGAATGAAAAAAAATAAA+AGG - chr8_1:68948562-68948581 Msa1210180:intron 10.0%
!! GTTCTATTTAAGTATAAAAA+AGG + chr8_1:68948082-68948101 None:intergenic 15.0%
!! ATAAGCATTTAACATATATT+AGG + chr8_1:68948104-68948123 None:intergenic 15.0%
!! GTTCTATTTAAGTATAAAAA+AGG + chr8_1:68948082-68948101 None:intergenic 15.0%
!! ATAAGCATTTAACATATATT+AGG + chr8_1:68948104-68948123 None:intergenic 15.0%
!!! TTTAAAATTTTCCTCTCTAA+TGG - chr8_1:68947627-68947646 Msa1210180:three_prime_UTR 20.0%
!! TTTCAACCAATAACTTAAAA+TGG + chr8_1:68947801-68947820 None:intergenic 20.0%
!! AGAAAGTAAAAAATGAATAC+CGG + chr8_1:68948021-68948040 None:intergenic 20.0%
!!! TTCATTTTTTACTTTCTTTC+TGG - chr8_1:68948024-68948043 Msa1210180:CDS 20.0%
!! GAATGAAAAAAAATAAAAGG+AGG - chr8_1:68948565-68948584 Msa1210180:intron 20.0%
!! CGTTTAATATTGTTGATATT+TGG - chr8_1:68948710-68948729 Msa1210180:five_prime_UTR 20.0%
!!! TTTAAAATTTTCCTCTCTAA+TGG - chr8_1:68947627-68947646 Msa1210180:three_prime_UTR 20.0%
!! TTTCAACCAATAACTTAAAA+TGG + chr8_1:68947801-68947820 None:intergenic 20.0%
!! AGAAAGTAAAAAATGAATAC+CGG + chr8_1:68948021-68948040 None:intergenic 20.0%
!!! TTCATTTTTTACTTTCTTTC+TGG - chr8_1:68948024-68948043 Msa1210180:CDS 20.0%
!! GAATGAAAAAAAATAAAAGG+AGG - chr8_1:68948565-68948584 Msa1210180:intron 20.0%
! AATGTTCAGTATAAGATGAA+AGG + chr8_1:68947723-68947742 None:intergenic 25.0%
!!! TTTGGACCATTTTAAGTTAT+TGG - chr8_1:68947792-68947811 Msa1210180:three_prime_UTR 25.0%
!! ATAAAAAAGGTGAAGTACTT+TGG + chr8_1:68948069-68948088 None:intergenic 25.0%
! ATTTACTAAACTACACTAGT+AGG + chr8_1:68948531-68948550 None:intergenic 25.0%
! TACTAGTGTAGTTTAGTAAA+TGG - chr8_1:68948530-68948549 Msa1210180:intron 25.0%
! ACTAGTGTAGTTTAGTAAAT+GGG - chr8_1:68948531-68948550 Msa1210180:intron 25.0%
! AATGTTCAGTATAAGATGAA+AGG + chr8_1:68947723-68947742 None:intergenic 25.0%
!!! TTTGGACCATTTTAAGTTAT+TGG - chr8_1:68947792-68947811 Msa1210180:three_prime_UTR 25.0%
!! ATAAAAAAGGTGAAGTACTT+TGG + chr8_1:68948069-68948088 None:intergenic 25.0%
! ATTTACTAAACTACACTAGT+AGG + chr8_1:68948531-68948550 None:intergenic 25.0%
! TACTAGTGTAGTTTAGTAAA+TGG - chr8_1:68948530-68948549 Msa1210180:intron 25.0%
! ACTAGTGTAGTTTAGTAAAT+GGG - chr8_1:68948531-68948550 Msa1210180:intron 25.0%
ATCTGATCATGAGATAGAAA+AGG - chr8_1:68947963-68947982 Msa1210180:CDS 30.0%
AATGCATTTAACTGATAAGC+CGG - chr8_1:68947999-68948018 Msa1210180:CDS 30.0%
TGTTAAACCTTTGTATTCGT+AGG - chr8_1:68948610-68948629 Msa1210180:intron 30.0%
TCAAAATCCTACGAATACAA+AGG + chr8_1:68948620-68948639 None:intergenic 30.0%
!! GTAGGATTTTGATGTATTCT+CGG - chr8_1:68948628-68948647 Msa1210180:intron 30.0%
TATGTTGTGGTAACTAACTA+TGG - chr8_1:68948657-68948676 Msa1210180:CDS 30.0%
ATCTGATCATGAGATAGAAA+AGG - chr8_1:68947963-68947982 Msa1210180:CDS 30.0%
AATGCATTTAACTGATAAGC+CGG - chr8_1:68947999-68948018 Msa1210180:CDS 30.0%
TGTTAAACCTTTGTATTCGT+AGG - chr8_1:68948610-68948629 Msa1210180:intron 30.0%
TCAAAATCCTACGAATACAA+AGG + chr8_1:68948620-68948639 None:intergenic 30.0%
!! GTAGGATTTTGATGTATTCT+CGG - chr8_1:68948628-68948647 Msa1210180:intron 30.0%
TATGTTGTGGTAACTAACTA+TGG - chr8_1:68948657-68948676 Msa1210180:CDS 30.0%
TACAAGTTAGTACCTTAGAG+AGG + chr8_1:68947696-68947715 None:intergenic 35.0%
GTTCAGTATAAGATGAAAGG+TGG + chr8_1:68947720-68947739 None:intergenic 35.0%
! GTGTTTTGTCAATATCATGC+AGG - chr8_1:68947936-68947955 Msa1210180:CDS 35.0%
! TTCTCGGTTTTTGTATGTTG+TGG - chr8_1:68948644-68948663 Msa1210180:CDS 35.0%
TACAAGTTAGTACCTTAGAG+AGG + chr8_1:68947696-68947715 None:intergenic 35.0%
GTTCAGTATAAGATGAAAGG+TGG + chr8_1:68947720-68947739 None:intergenic 35.0%
! GTGTTTTGTCAATATCATGC+AGG - chr8_1:68947936-68947955 Msa1210180:CDS 35.0%
! TTCTCGGTTTTTGTATGTTG+TGG - chr8_1:68948644-68948663 Msa1210180:CDS 35.0%
TTACGCAAGCTTCTTGTCTT+CGG - chr8_1:68947652-68947671 Msa1210180:three_prime_UTR 40.0%
AGAAGGCTAATGCAAGACAT+AGG - chr8_1:68948162-68948181 Msa1210180:CDS 40.0%
! GATCAATTTTGTGCAGACTG+TGG - chr8_1:68948267-68948286 Msa1210180:intron 40.0%
! AATTTTGTGCAGACTGTGGA+GGG - chr8_1:68948271-68948290 Msa1210180:intron 40.0%
CATGCTCTCTTGCACAAATT+TGG + chr8_1:68948330-68948349 None:intergenic 40.0%
! AATTTGTGCAAGAGAGCATG+TGG - chr8_1:68948330-68948349 Msa1210180:CDS 40.0%
! ATTTGTGCAAGAGAGCATGT+GGG - chr8_1:68948331-68948350 Msa1210180:CDS 40.0%
TCCACAAAACTCCCTATTAC+CGG + chr8_1:68948397-68948416 None:intergenic 40.0%
TACACTGATATGACCACTCA+TGG - chr8_1:68948423-68948442 Msa1210180:intron 40.0%
! GGCTTTGATAAGGTCTCTAA+GGG + chr8_1:68948464-68948483 None:intergenic 40.0%
GAAGAGAGCCCAAACTAATT+AGG + chr8_1:68948493-68948512 None:intergenic 40.0%
TACACTAGTAGGTACTACGT+GGG + chr8_1:68948520-68948539 None:intergenic 40.0%
TTACGCAAGCTTCTTGTCTT+CGG - chr8_1:68947652-68947671 Msa1210180:three_prime_UTR 40.0%
AGAAGGCTAATGCAAGACAT+AGG - chr8_1:68948162-68948181 Msa1210180:CDS 40.0%
! GATCAATTTTGTGCAGACTG+TGG - chr8_1:68948267-68948286 Msa1210180:intron 40.0%
! AATTTTGTGCAGACTGTGGA+GGG - chr8_1:68948271-68948290 Msa1210180:intron 40.0%
CATGCTCTCTTGCACAAATT+TGG + chr8_1:68948330-68948349 None:intergenic 40.0%
! AATTTGTGCAAGAGAGCATG+TGG - chr8_1:68948330-68948349 Msa1210180:CDS 40.0%
! ATTTGTGCAAGAGAGCATGT+GGG - chr8_1:68948331-68948350 Msa1210180:CDS 40.0%
TCCACAAAACTCCCTATTAC+CGG + chr8_1:68948397-68948416 None:intergenic 40.0%
TACACTGATATGACCACTCA+TGG - chr8_1:68948423-68948442 Msa1210180:intron 40.0%
! GGCTTTGATAAGGTCTCTAA+GGG + chr8_1:68948464-68948483 None:intergenic 40.0%
GAAGAGAGCCCAAACTAATT+AGG + chr8_1:68948493-68948512 None:intergenic 40.0%
TACACTAGTAGGTACTACGT+GGG + chr8_1:68948520-68948539 None:intergenic 40.0%
CTTGCGTAATGCCATTAGAG+AGG + chr8_1:68947641-68947660 None:intergenic 45.0%
TACGCAAGCTTCTTGTCTTC+GGG - chr8_1:68947653-68947672 Msa1210180:three_prime_UTR 45.0%
! GCTTTTAGTGTGCCTCTCTA+AGG - chr8_1:68947681-68947700 Msa1210180:three_prime_UTR 45.0%
AAGCCCTTGGTTTATGTCGT+GGG - chr8_1:68947765-68947784 Msa1210180:three_prime_UTR 45.0%
!! GTTTATGTCGTGGGGTGTTT+TGG - chr8_1:68947774-68947793 Msa1210180:three_prime_UTR 45.0%
CTTCAGCTTATTGTGCGAGA+CGG - chr8_1:68948135-68948154 Msa1210180:intron 45.0%
! CAATTTTGTGCAGACTGTGG+AGG - chr8_1:68948270-68948289 Msa1210180:intron 45.0%
!! CTTGGTCTTGTTACCATGAG+TGG + chr8_1:68948439-68948458 None:intergenic 45.0%
!! ATAAGGTCTCTAAGGGCACT+TGG + chr8_1:68948457-68948476 None:intergenic 45.0%
! GGGCTTTGATAAGGTCTCTA+AGG + chr8_1:68948465-68948484 None:intergenic 45.0%
AGAGACCTTATCAAAGCCCA+TGG - chr8_1:68948466-68948485 Msa1210180:intron 45.0%
GAGACCTTATCAAAGCCCAT+GGG - chr8_1:68948467-68948486 Msa1210180:intron 45.0%
CCCAAACTAATTAGGCCCAT+GGG + chr8_1:68948485-68948504 None:intergenic 45.0%
! CCCATGGGCCTAATTAGTTT+GGG - chr8_1:68948482-68948501 Msa1210180:intron 45.0%
CTACACTAGTAGGTACTACG+TGG + chr8_1:68948521-68948540 None:intergenic 45.0%
CTTGCGTAATGCCATTAGAG+AGG + chr8_1:68947641-68947660 None:intergenic 45.0%
TACGCAAGCTTCTTGTCTTC+GGG - chr8_1:68947653-68947672 Msa1210180:three_prime_UTR 45.0%
! GCTTTTAGTGTGCCTCTCTA+AGG - chr8_1:68947681-68947700 Msa1210180:three_prime_UTR 45.0%
AAGCCCTTGGTTTATGTCGT+GGG - chr8_1:68947765-68947784 Msa1210180:three_prime_UTR 45.0%
!! GTTTATGTCGTGGGGTGTTT+TGG - chr8_1:68947774-68947793 Msa1210180:three_prime_UTR 45.0%
CTTCAGCTTATTGTGCGAGA+CGG - chr8_1:68948135-68948154 Msa1210180:intron 45.0%
! CAATTTTGTGCAGACTGTGG+AGG - chr8_1:68948270-68948289 Msa1210180:intron 45.0%
!! CTTGGTCTTGTTACCATGAG+TGG + chr8_1:68948439-68948458 None:intergenic 45.0%
!! ATAAGGTCTCTAAGGGCACT+TGG + chr8_1:68948457-68948476 None:intergenic 45.0%
! GGGCTTTGATAAGGTCTCTA+AGG + chr8_1:68948465-68948484 None:intergenic 45.0%
AGAGACCTTATCAAAGCCCA+TGG - chr8_1:68948466-68948485 Msa1210180:intron 45.0%
GAGACCTTATCAAAGCCCAT+GGG - chr8_1:68948467-68948486 Msa1210180:intron 45.0%
CCCAAACTAATTAGGCCCAT+GGG + chr8_1:68948485-68948504 None:intergenic 45.0%
! CCCATGGGCCTAATTAGTTT+GGG - chr8_1:68948482-68948501 Msa1210180:intron 45.0%
CTACACTAGTAGGTACTACG+TGG + chr8_1:68948521-68948540 None:intergenic 45.0%
ACTGCTCTGTTTCAAGCCCT+TGG - chr8_1:68947752-68947771 Msa1210180:three_prime_UTR 50.0%
CAAGCCCTTGGTTTATGTCG+TGG - chr8_1:68947764-68947783 Msa1210180:three_prime_UTR 50.0%
AGCCCTTGGTTTATGTCGTG+GGG - chr8_1:68947766-68947785 Msa1210180:three_prime_UTR 50.0%
CACCCCACGACATAAACCAA+GGG + chr8_1:68947771-68947790 None:intergenic 50.0%
ACACCCCACGACATAAACCA+AGG + chr8_1:68947772-68947791 None:intergenic 50.0%
TTGTGCGAGACGGAAACAGA+AGG - chr8_1:68948145-68948164 Msa1210180:intron 50.0%
CAAGGTGCAGTGTGCATTCA+AGG - chr8_1:68948304-68948323 Msa1210180:intron 50.0%
TATTACCGGCAGTACCAGGT+GGG + chr8_1:68948383-68948402 None:intergenic 50.0%
ACCTGGTACTGCCGGTAATA+GGG - chr8_1:68948383-68948402 Msa1210180:intron 50.0%
! GCCGGTAATAGGGAGTTTTG+TGG - chr8_1:68948393-68948412 Msa1210180:intron 50.0%
! TAGGCCCATGGGCTTTGATA+AGG + chr8_1:68948474-68948493 None:intergenic 50.0%
GCCCATGGGCCTAATTAGTT+TGG - chr8_1:68948481-68948500 Msa1210180:intron 50.0%
GCCCAAACTAATTAGGCCCA+TGG + chr8_1:68948486-68948505 None:intergenic 50.0%
ACTGCTCTGTTTCAAGCCCT+TGG - chr8_1:68947752-68947771 Msa1210180:three_prime_UTR 50.0%
CAAGCCCTTGGTTTATGTCG+TGG - chr8_1:68947764-68947783 Msa1210180:three_prime_UTR 50.0%
AGCCCTTGGTTTATGTCGTG+GGG - chr8_1:68947766-68947785 Msa1210180:three_prime_UTR 50.0%
CACCCCACGACATAAACCAA+GGG + chr8_1:68947771-68947790 None:intergenic 50.0%
ACACCCCACGACATAAACCA+AGG + chr8_1:68947772-68947791 None:intergenic 50.0%
TTGTGCGAGACGGAAACAGA+AGG - chr8_1:68948145-68948164 Msa1210180:intron 50.0%
CAAGGTGCAGTGTGCATTCA+AGG - chr8_1:68948304-68948323 Msa1210180:intron 50.0%
TATTACCGGCAGTACCAGGT+GGG + chr8_1:68948383-68948402 None:intergenic 50.0%
ACCTGGTACTGCCGGTAATA+GGG - chr8_1:68948383-68948402 Msa1210180:intron 50.0%
! GCCGGTAATAGGGAGTTTTG+TGG - chr8_1:68948393-68948412 Msa1210180:intron 50.0%
! TAGGCCCATGGGCTTTGATA+AGG + chr8_1:68948474-68948493 None:intergenic 50.0%
GCCCATGGGCCTAATTAGTT+TGG - chr8_1:68948481-68948500 Msa1210180:intron 50.0%
GCCCAAACTAATTAGGCCCA+TGG + chr8_1:68948486-68948505 None:intergenic 50.0%
!! CATGTGGGACTTGCTGTGTG+AGG - chr8_1:68948346-68948365 Msa1210180:CDS 55.0%
CTATTACCGGCAGTACCAGG+TGG + chr8_1:68948384-68948403 None:intergenic 55.0%
CACCTGGTACTGCCGGTAAT+AGG - chr8_1:68948382-68948401 Msa1210180:intron 55.0%
TCCCTATTACCGGCAGTACC+AGG + chr8_1:68948387-68948406 None:intergenic 55.0%
!! CATGTGGGACTTGCTGTGTG+AGG - chr8_1:68948346-68948365 Msa1210180:CDS 55.0%
CTATTACCGGCAGTACCAGG+TGG + chr8_1:68948384-68948403 None:intergenic 55.0%
CACCTGGTACTGCCGGTAAT+AGG - chr8_1:68948382-68948401 Msa1210180:intron 55.0%
TCCCTATTACCGGCAGTACC+AGG + chr8_1:68948387-68948406 None:intergenic 55.0%
CAGACTGTGGAGGGCTATGC+AGG - chr8_1:68948280-68948299 Msa1210180:intron 60.0%
GTGGAGGGCTATGCAGGTCA+AGG - chr8_1:68948286-68948305 Msa1210180:intron 60.0%
AGGTGCAAGTGTGTCCCACC+TGG - chr8_1:68948366-68948385 Msa1210180:intron 60.0%
TGTGTCCCACCTGGTACTGC+CGG - chr8_1:68948375-68948394 Msa1210180:intron 60.0%
CAGACTGTGGAGGGCTATGC+AGG - chr8_1:68948280-68948299 Msa1210180:intron 60.0%
GTGGAGGGCTATGCAGGTCA+AGG - chr8_1:68948286-68948305 Msa1210180:intron 60.0%
AGGTGCAAGTGTGTCCCACC+TGG - chr8_1:68948366-68948385 Msa1210180:intron 60.0%
TGTGTCCCACCTGGTACTGC+CGG - chr8_1:68948375-68948394 Msa1210180:intron 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 68947606 68948732 68947606 ID=Msa1210180;Name=Msa1210180
chr8_1 mRNA 68947606 68948732 68947606 ID=Msa1210180-mRNA-1;Parent=Msa1210180;Name=Msa1210180-mRNA-1;_AED=0.14;_eAED=0.14;_QI=40|1|1|1|1|1|4|265|115
chr8_1 exon 68947606 68948055 68947606 ID=Msa1210180-mRNA-1:exon:23065;Parent=Msa1210180-mRNA-1
chr8_1 exon 68948155 68948197 68948155 ID=Msa1210180-mRNA-1:exon:23064;Parent=Msa1210180-mRNA-1
chr8_1 exon 68948318 68948380 68948318 ID=Msa1210180-mRNA-1:exon:23063;Parent=Msa1210180-mRNA-1
chr8_1 exon 68948636 68948732 68948636 ID=Msa1210180-mRNA-1:exon:23062;Parent=Msa1210180-mRNA-1
chr8_1 five_prime_UTR 68948693 68948732 68948693 ID=Msa1210180-mRNA-1:five_prime_utr;Parent=Msa1210180-mRNA-1
chr8_1 CDS 68948636 68948692 68948636 ID=Msa1210180-mRNA-1:cds;Parent=Msa1210180-mRNA-1
chr8_1 CDS 68948318 68948380 68948318 ID=Msa1210180-mRNA-1:cds;Parent=Msa1210180-mRNA-1
chr8_1 CDS 68948155 68948197 68948155 ID=Msa1210180-mRNA-1:cds;Parent=Msa1210180-mRNA-1
chr8_1 CDS 68947871 68948055 68947871 ID=Msa1210180-mRNA-1:cds;Parent=Msa1210180-mRNA-1
chr8_1 three_prime_UTR 68947606 68947870 68947606 ID=Msa1210180-mRNA-1:three_prime_utr;Parent=Msa1210180-mRNA-1
Gene Sequence

>Msa1210180

ATGGCATTACGCAAGCTTCTTGTCTTCGGGTTGTTGCTTTTAGTGTGCCTCTCTAAGGTTTCATCTGATCATGAGATAGAAAAGGAAGAAGATGAAGAAATGCATTTAACTGATAAGCCGCTTATTGTGCGAGACGGAAACAGAAGGCTAATGCAAGACATAGACTGTGGAGGGCTATGCAGGTCAAGGTGCAGTGTGCATTCAAGGCCAAATTTGTGCAAGAGAGCATGTGGGACTTGCTGTGTGAGGTGCAAGTGTGTCCCACCTGGTACTGCCGGTAATAGGGAGTTTTGTGGAGCTTGTTACACTGATATGACCACTCATGGTAACAAGACCAAGTGCCCTTAG
Protein sequence

>Msa1210180

MALRKLLVFGLLLLVCLSKVSSDHEIEKEEDEEMHLTDKPLIVRDGNRRLMQDIDCGGLCRSRCSVHSRPNLCKRACGTCCVRCKCVPPGTAGNREFCGACYTDMTTHGNKTKCP*