AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1259290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1259290 MtrunA17_Chr4g0059421 99.130 115 1 0 1 115 1 115 1.29e-80 231
Msa1259290 MtrunA17_Chr8g0364311 56.364 110 44 1 6 115 7 112 1.52e-38 125
Msa1259290 MtrunA17_Chr3g0142671 47.826 115 48 3 1 115 1 103 4.53e-28 98.6
Msa1259290 MtrunA17_Chr1g0156601 46.957 115 47 3 1 115 1 101 7.41e-27 95.5
Msa1259290 MtrunA17_Chr6g0451481 72.131 61 17 0 55 115 159 219 4.75e-25 94.4
Msa1259290 MtrunA17_Chr1g0156621 63.636 66 23 1 50 115 36 100 7.49e-23 85.1
Msa1259290 MtrunA17_Chr8g0371941 62.121 66 24 1 50 115 39 103 1.17e-21 82.4
Msa1259290 MtrunA17_Chr1g0200531 50.000 76 36 2 41 115 26 100 2.19e-19 76.6
Msa1259290 MtrunA17_Chr6g0451501 57.377 61 26 0 55 115 102 162 3.38e-17 72.4
Msa1259290 MtrunA17_Chr6g0451491 59.677 62 24 1 55 115 69 130 3.67e-17 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1259290 AT5G14920.1 66.129 62 21 0 54 115 214 275 2.04e-22 89.0
Msa1259290 AT4G09600.1 64.516 62 21 1 54 115 39 99 9.39e-22 82.8
Msa1259290 AT2G18420.1 62.903 62 22 1 54 115 34 94 8.00e-21 80.5
Msa1259290 AT2G18420.2 62.903 62 22 1 54 115 10 70 8.04e-21 79.7
Msa1259290 AT1G75750.1 57.576 66 27 1 50 115 34 98 1.09e-20 80.1
Msa1259290 AT1G75750.2 57.576 66 27 1 50 115 33 97 1.27e-20 79.7
Msa1259290 AT1G22690.1 58.065 62 25 1 54 115 59 119 6.00e-19 76.3
Msa1259290 AT1G22690.2 58.065 62 25 1 54 115 59 119 6.00e-19 76.3
Msa1259290 AT1G22690.3 58.065 62 25 1 54 115 59 119 6.00e-19 76.3
Msa1259290 AT4G09610.1 61.290 62 23 1 54 115 39 99 3.67e-13 60.8
Msa1259290 AT1G74670.1 31.858 113 65 2 3 115 1 101 2.44e-11 56.2

Find 48 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTTAATATTGTTGATATT+TGG 0.162273 8_2:-60856137 Msa1259290:three_prime_UTR
CATGCTCTCTTGCACAAATT+TGG 0.199596 8_2:+60856520 None:intergenic
TACTAGTGTAGTTTGGTAAA+TGG 0.244704 8_2:-60856317 Msa1259290:three_prime_UTR
TTTAAAATTCTCCTCTCTAA+TGG 0.280437 8_2:-60857202 Msa1259290:exon
GCCCATGGGCCTAATTAGTT+TGG 0.284816 8_2:-60856366 Msa1259290:three_prime_UTR
GGGCTTTGATAAGGTCTCTA+AGG 0.305310 8_2:+60856385 None:intergenic
TTACGCAAGCTTCTTGTCTT+CGG 0.310911 8_2:-60857177 Msa1259290:CDS
GAAGAGAGCCCAAACTAATT+AGG 0.320634 8_2:+60856357 None:intergenic
TACGCAAGCTTCTTGTCTTC+GGG 0.326759 8_2:-60857176 Msa1259290:CDS
CACCTGGTACTGCCGGTAAT+AGG 0.335185 8_2:-60856465 Msa1259290:CDS
TATGTTGTGGTAACTAACTA+TGG 0.368562 8_2:-60856190 Msa1259290:three_prime_UTR
ACCTGGTACTGCCGGTAATA+GGG 0.401958 8_2:-60856464 Msa1259290:CDS
CCCATGGGCCTAATTAGTTT+GGG 0.408002 8_2:-60856365 Msa1259290:three_prime_UTR
TCCACAAAACTCCCTATTAC+CGG 0.431014 8_2:+60856453 None:intergenic
ACTAGTGTAGTTTGGTAAAT+GGG 0.439046 8_2:-60856316 Msa1259290:three_prime_UTR
TAGGCCCATGGGCTTTGATA+AGG 0.442762 8_2:+60856376 None:intergenic
CAAGGTGCAGTGTGCATTCA+AGG 0.442762 8_2:-60856543 Msa1259290:CDS
CAGACTGTGGAGGGCTATGC+AGG 0.492139 8_2:-60856567 Msa1259290:CDS
TAGTACCTACTAGTGTAGTT+TGG 0.501441 8_2:-60856324 Msa1259290:three_prime_UTR
ATTTACCAAACTACACTAGT+AGG 0.502074 8_2:+60856319 None:intergenic
CTTGGTCTTGTTACCATGAG+TGG 0.515862 8_2:+60856411 None:intergenic
ATCTGATCATGAGATAGAAA+AGG 0.540603 8_2:-60856886 Msa1259290:CDS
TCCCTATTACCGGCAGTACC+AGG 0.543014 8_2:+60856463 None:intergenic
AATGCATTTAACTGATAAGC+CGG 0.550151 8_2:-60856850 Msa1259290:intron
GCCCAAACTAATTAGGCCCA+TGG 0.551582 8_2:+60856364 None:intergenic
ATAAGGTCTCTAAGGGCACT+TGG 0.552905 8_2:+60856393 None:intergenic
TGTGTCCCACCTGGTACTGC+CGG 0.553087 8_2:-60856472 Msa1259290:CDS
GGCTTTGATAAGGTCTCTAA+GGG 0.558100 8_2:+60856386 None:intergenic
CTTCAGCTTATTGTGCGAGA+CGG 0.558872 8_2:-60856712 Msa1259290:intron
AGAGACCTTATCAAAGCCCA+TGG 0.565394 8_2:-60856381 Msa1259290:three_prime_UTR
TACACTGATATGACCACTCA+TGG 0.567522 8_2:-60856424 Msa1259290:CDS
TGTTAAACCTTTGTATTCGT+AGG 0.570058 8_2:-60856237 Msa1259290:three_prime_UTR
CCCAAACTAATTAGGCCCAT+GGG 0.581285 8_2:+60856365 None:intergenic
AGAAGGCTAATGCAAGACAT+AGG 0.585779 8_2:-60856685 Msa1259290:intron
AGGTGCAAGTGTGTCCCACC+TGG 0.590839 8_2:-60856481 Msa1259290:CDS
AATTTGTGCAAGAGAGCATG+TGG 0.594899 8_2:-60856517 Msa1259290:CDS
GTGGAGGGCTATGCAGGTCA+AGG 0.600120 8_2:-60856561 Msa1259290:CDS
TTGTGCGAGACGGAAACAGA+AGG 0.605905 8_2:-60856702 Msa1259290:CDS
TCAAAATCCTACGAATACAA+AGG 0.641840 8_2:+60856230 None:intergenic
CATGTGGGACTTGCTGTGTG+AGG 0.647718 8_2:-60856501 Msa1259290:CDS
TACAAGTTAGTACCTTAGAG+AGG 0.650629 8_2:+60857136 None:intergenic
CTTGCGTAATGCCATTAGAG+AGG 0.657310 8_2:+60857191 None:intergenic
GAGACCTTATCAAAGCCCAT+GGG 0.661282 8_2:-60856380 Msa1259290:three_prime_UTR
TACACTAGTAGGTACTACGT+GGG 0.679336 8_2:+60856330 None:intergenic
CTACACTAGTAGGTACTACG+TGG 0.684236 8_2:+60856329 None:intergenic
CTATTACCGGCAGTACCAGG+TGG 0.706350 8_2:+60856466 None:intergenic
TATTACCGGCAGTACCAGGT+GGG 0.719553 8_2:+60856467 None:intergenic
ATTTGTGCAAGAGAGCATGT+GGG 0.729137 8_2:-60856516 Msa1259290:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTATATTTAGTTTTATTTTT+TGG + chr8_2:60856704-60856723 None:intergenic 10.0%
!! TTTGAATGAAAAAAAATAAA+AGG - chr8_2:60857074-60857093 Msa1259290:intron 10.0%
!!! GTATATTTAGTTTTATTTTT+TGG + chr8_2:60856704-60856723 None:intergenic 10.0%
!! TTTGAATGAAAAAAAATAAA+AGG - chr8_2:60857074-60857093 Msa1259290:intron 10.0%
!! GTTCTATTTAAGTATAAAAA+AGG + chr8_2:60856594-60856613 None:intergenic 15.0%
!! ATAAGCATTTAACATATATT+AGG + chr8_2:60856616-60856635 None:intergenic 15.0%
!! GTTCTATTTAAGTATAAAAA+AGG + chr8_2:60856594-60856613 None:intergenic 15.0%
!! ATAAGCATTTAACATATATT+AGG + chr8_2:60856616-60856635 None:intergenic 15.0%
!! TTTCAACCAATAACTTAAAA+TGG + chr8_2:60856331-60856350 None:intergenic 20.0%
!! AGAAAGTAAAAAATGAATAC+CGG + chr8_2:60856531-60856550 None:intergenic 20.0%
!!! TTCATTTTTTACTTTCTTTC+TGG - chr8_2:60856534-60856553 Msa1259290:CDS 20.0%
!! GAATGAAAAAAAATAAAAGG+AGG - chr8_2:60857077-60857096 Msa1259290:intron 20.0%
!! CGTTTAATATTGTTGATATT+TGG - chr8_2:60857222-60857241 Msa1259290:five_prime_UTR 20.0%
!! TTTCAACCAATAACTTAAAA+TGG + chr8_2:60856331-60856350 None:intergenic 20.0%
!! AGAAAGTAAAAAATGAATAC+CGG + chr8_2:60856531-60856550 None:intergenic 20.0%
!!! TTCATTTTTTACTTTCTTTC+TGG - chr8_2:60856534-60856553 Msa1259290:CDS 20.0%
!! GAATGAAAAAAAATAAAAGG+AGG - chr8_2:60857077-60857096 Msa1259290:intron 20.0%
!! TTTAAAATTCTCCTCTCTAA+TGG - chr8_2:60856157-60856176 Msa1259290:three_prime_UTR 25.0%
! AATGTTCAGTATAAGATGAA+AGG + chr8_2:60856253-60856272 None:intergenic 25.0%
!!! TTTGGACCATTTTAAGTTAT+TGG - chr8_2:60856322-60856341 Msa1259290:three_prime_UTR 25.0%
!! ATAAAAAAGGTGAAGTACTT+TGG + chr8_2:60856581-60856600 None:intergenic 25.0%
!! TTTAAAATTCTCCTCTCTAA+TGG - chr8_2:60856157-60856176 Msa1259290:three_prime_UTR 25.0%
! AATGTTCAGTATAAGATGAA+AGG + chr8_2:60856253-60856272 None:intergenic 25.0%
!!! TTTGGACCATTTTAAGTTAT+TGG - chr8_2:60856322-60856341 Msa1259290:three_prime_UTR 25.0%
!! ATAAAAAAGGTGAAGTACTT+TGG + chr8_2:60856581-60856600 None:intergenic 25.0%
ATCTGATCATGAGATAGAAA+AGG - chr8_2:60856473-60856492 Msa1259290:CDS 30.0%
AATGCATTTAACTGATAAGC+CGG - chr8_2:60856509-60856528 Msa1259290:CDS 30.0%
ATTTACCAAACTACACTAGT+AGG + chr8_2:60857043-60857062 None:intergenic 30.0%
TACTAGTGTAGTTTGGTAAA+TGG - chr8_2:60857042-60857061 Msa1259290:intron 30.0%
ACTAGTGTAGTTTGGTAAAT+GGG - chr8_2:60857043-60857062 Msa1259290:intron 30.0%
TGTTAAACCTTTGTATTCGT+AGG - chr8_2:60857122-60857141 Msa1259290:intron 30.0%
TCAAAATCCTACGAATACAA+AGG + chr8_2:60857132-60857151 None:intergenic 30.0%
!! GTAGGATTTTGATGTATTCT+CGG - chr8_2:60857140-60857159 Msa1259290:intron 30.0%
TATGTTGTGGTAACTAACTA+TGG - chr8_2:60857169-60857188 Msa1259290:CDS 30.0%
ATCTGATCATGAGATAGAAA+AGG - chr8_2:60856473-60856492 Msa1259290:CDS 30.0%
AATGCATTTAACTGATAAGC+CGG - chr8_2:60856509-60856528 Msa1259290:CDS 30.0%
ATTTACCAAACTACACTAGT+AGG + chr8_2:60857043-60857062 None:intergenic 30.0%
TACTAGTGTAGTTTGGTAAA+TGG - chr8_2:60857042-60857061 Msa1259290:intron 30.0%
ACTAGTGTAGTTTGGTAAAT+GGG - chr8_2:60857043-60857062 Msa1259290:intron 30.0%
TGTTAAACCTTTGTATTCGT+AGG - chr8_2:60857122-60857141 Msa1259290:intron 30.0%
TCAAAATCCTACGAATACAA+AGG + chr8_2:60857132-60857151 None:intergenic 30.0%
!! GTAGGATTTTGATGTATTCT+CGG - chr8_2:60857140-60857159 Msa1259290:intron 30.0%
TATGTTGTGGTAACTAACTA+TGG - chr8_2:60857169-60857188 Msa1259290:CDS 30.0%
TACAAGTTAGTACCTTAGAG+AGG + chr8_2:60856226-60856245 None:intergenic 35.0%
GTTCAGTATAAGATGAAAGG+TGG + chr8_2:60856250-60856269 None:intergenic 35.0%
! GTGTTTTGTCAATATCATGC+AGG - chr8_2:60856446-60856465 Msa1259290:CDS 35.0%
TAGTACCTACTAGTGTAGTT+TGG - chr8_2:60857035-60857054 Msa1259290:intron 35.0%
! TTCTCGGTTTTTGTATGTTG+TGG - chr8_2:60857156-60857175 Msa1259290:CDS 35.0%
TACAAGTTAGTACCTTAGAG+AGG + chr8_2:60856226-60856245 None:intergenic 35.0%
GTTCAGTATAAGATGAAAGG+TGG + chr8_2:60856250-60856269 None:intergenic 35.0%
! GTGTTTTGTCAATATCATGC+AGG - chr8_2:60856446-60856465 Msa1259290:CDS 35.0%
TAGTACCTACTAGTGTAGTT+TGG - chr8_2:60857035-60857054 Msa1259290:intron 35.0%
! TTCTCGGTTTTTGTATGTTG+TGG - chr8_2:60857156-60857175 Msa1259290:CDS 35.0%
TTACGCAAGCTTCTTGTCTT+CGG - chr8_2:60856182-60856201 Msa1259290:three_prime_UTR 40.0%
AAGCCCTTGGTTTATGTCAT+GGG - chr8_2:60856295-60856314 Msa1259290:three_prime_UTR 40.0%
!! GTTTATGTCATGGGGTGTTT+TGG - chr8_2:60856304-60856323 Msa1259290:three_prime_UTR 40.0%
AGAAGGCTAATGCAAGACAT+AGG - chr8_2:60856674-60856693 Msa1259290:intron 40.0%
! GATCAATTTTGTGCAGACTG+TGG - chr8_2:60856779-60856798 Msa1259290:intron 40.0%
! AATTTTGTGCAGACTGTGGA+GGG - chr8_2:60856783-60856802 Msa1259290:intron 40.0%
CATGCTCTCTTGCACAAATT+TGG + chr8_2:60856842-60856861 None:intergenic 40.0%
! AATTTGTGCAAGAGAGCATG+TGG - chr8_2:60856842-60856861 Msa1259290:intron 40.0%
! ATTTGTGCAAGAGAGCATGT+GGG - chr8_2:60856843-60856862 Msa1259290:intron 40.0%
TCCACAAAACTCCCTATTAC+CGG + chr8_2:60856909-60856928 None:intergenic 40.0%
TACACTGATATGACCACTCA+TGG - chr8_2:60856935-60856954 Msa1259290:intron 40.0%
! GGCTTTGATAAGGTCTCTAA+GGG + chr8_2:60856976-60856995 None:intergenic 40.0%
GAAGAGAGCCCAAACTAATT+AGG + chr8_2:60857005-60857024 None:intergenic 40.0%
TACACTAGTAGGTACTACGT+GGG + chr8_2:60857032-60857051 None:intergenic 40.0%
TTACGCAAGCTTCTTGTCTT+CGG - chr8_2:60856182-60856201 Msa1259290:three_prime_UTR 40.0%
AAGCCCTTGGTTTATGTCAT+GGG - chr8_2:60856295-60856314 Msa1259290:three_prime_UTR 40.0%
!! GTTTATGTCATGGGGTGTTT+TGG - chr8_2:60856304-60856323 Msa1259290:three_prime_UTR 40.0%
AGAAGGCTAATGCAAGACAT+AGG - chr8_2:60856674-60856693 Msa1259290:intron 40.0%
! GATCAATTTTGTGCAGACTG+TGG - chr8_2:60856779-60856798 Msa1259290:intron 40.0%
! AATTTTGTGCAGACTGTGGA+GGG - chr8_2:60856783-60856802 Msa1259290:intron 40.0%
CATGCTCTCTTGCACAAATT+TGG + chr8_2:60856842-60856861 None:intergenic 40.0%
! AATTTGTGCAAGAGAGCATG+TGG - chr8_2:60856842-60856861 Msa1259290:intron 40.0%
! ATTTGTGCAAGAGAGCATGT+GGG - chr8_2:60856843-60856862 Msa1259290:intron 40.0%
TCCACAAAACTCCCTATTAC+CGG + chr8_2:60856909-60856928 None:intergenic 40.0%
TACACTGATATGACCACTCA+TGG - chr8_2:60856935-60856954 Msa1259290:intron 40.0%
! GGCTTTGATAAGGTCTCTAA+GGG + chr8_2:60856976-60856995 None:intergenic 40.0%
GAAGAGAGCCCAAACTAATT+AGG + chr8_2:60857005-60857024 None:intergenic 40.0%
TACACTAGTAGGTACTACGT+GGG + chr8_2:60857032-60857051 None:intergenic 40.0%
CTTGCGTAATGCCATTAGAG+AGG + chr8_2:60856171-60856190 None:intergenic 45.0%
TACGCAAGCTTCTTGTCTTC+GGG - chr8_2:60856183-60856202 Msa1259290:three_prime_UTR 45.0%
! GCTTTTAGTGTGCCTCTCTA+AGG - chr8_2:60856211-60856230 Msa1259290:three_prime_UTR 45.0%
CAAGCCCTTGGTTTATGTCA+TGG - chr8_2:60856294-60856313 Msa1259290:three_prime_UTR 45.0%
AGCCCTTGGTTTATGTCATG+GGG - chr8_2:60856296-60856315 Msa1259290:three_prime_UTR 45.0%
CACCCCATGACATAAACCAA+GGG + chr8_2:60856301-60856320 None:intergenic 45.0%
ACACCCCATGACATAAACCA+AGG + chr8_2:60856302-60856321 None:intergenic 45.0%
CTTCAGCTTATTGTGCGAGA+CGG - chr8_2:60856647-60856666 Msa1259290:intron 45.0%
! CAATTTTGTGCAGACTGTGG+AGG - chr8_2:60856782-60856801 Msa1259290:intron 45.0%
!! CTTGGTCTTGTTACCATGAG+TGG + chr8_2:60856951-60856970 None:intergenic 45.0%
!! ATAAGGTCTCTAAGGGCACT+TGG + chr8_2:60856969-60856988 None:intergenic 45.0%
! GGGCTTTGATAAGGTCTCTA+AGG + chr8_2:60856977-60856996 None:intergenic 45.0%
AGAGACCTTATCAAAGCCCA+TGG - chr8_2:60856978-60856997 Msa1259290:intron 45.0%
GAGACCTTATCAAAGCCCAT+GGG - chr8_2:60856979-60856998 Msa1259290:intron 45.0%
CCCAAACTAATTAGGCCCAT+GGG + chr8_2:60856997-60857016 None:intergenic 45.0%
! CCCATGGGCCTAATTAGTTT+GGG - chr8_2:60856994-60857013 Msa1259290:intron 45.0%
CTACACTAGTAGGTACTACG+TGG + chr8_2:60857033-60857052 None:intergenic 45.0%
CTTGCGTAATGCCATTAGAG+AGG + chr8_2:60856171-60856190 None:intergenic 45.0%
TACGCAAGCTTCTTGTCTTC+GGG - chr8_2:60856183-60856202 Msa1259290:three_prime_UTR 45.0%
! GCTTTTAGTGTGCCTCTCTA+AGG - chr8_2:60856211-60856230 Msa1259290:three_prime_UTR 45.0%
CAAGCCCTTGGTTTATGTCA+TGG - chr8_2:60856294-60856313 Msa1259290:three_prime_UTR 45.0%
AGCCCTTGGTTTATGTCATG+GGG - chr8_2:60856296-60856315 Msa1259290:three_prime_UTR 45.0%
CACCCCATGACATAAACCAA+GGG + chr8_2:60856301-60856320 None:intergenic 45.0%
ACACCCCATGACATAAACCA+AGG + chr8_2:60856302-60856321 None:intergenic 45.0%
CTTCAGCTTATTGTGCGAGA+CGG - chr8_2:60856647-60856666 Msa1259290:intron 45.0%
! CAATTTTGTGCAGACTGTGG+AGG - chr8_2:60856782-60856801 Msa1259290:intron 45.0%
!! CTTGGTCTTGTTACCATGAG+TGG + chr8_2:60856951-60856970 None:intergenic 45.0%
!! ATAAGGTCTCTAAGGGCACT+TGG + chr8_2:60856969-60856988 None:intergenic 45.0%
! GGGCTTTGATAAGGTCTCTA+AGG + chr8_2:60856977-60856996 None:intergenic 45.0%
AGAGACCTTATCAAAGCCCA+TGG - chr8_2:60856978-60856997 Msa1259290:intron 45.0%
GAGACCTTATCAAAGCCCAT+GGG - chr8_2:60856979-60856998 Msa1259290:intron 45.0%
CCCAAACTAATTAGGCCCAT+GGG + chr8_2:60856997-60857016 None:intergenic 45.0%
! CCCATGGGCCTAATTAGTTT+GGG - chr8_2:60856994-60857013 Msa1259290:intron 45.0%
CTACACTAGTAGGTACTACG+TGG + chr8_2:60857033-60857052 None:intergenic 45.0%
ACTGCTCTGTTTCAAGCCCT+TGG - chr8_2:60856282-60856301 Msa1259290:three_prime_UTR 50.0%
TTGTGCGAGACGGAAACAGA+AGG - chr8_2:60856657-60856676 Msa1259290:intron 50.0%
CAAGGTGCAGTGTGCATTCA+AGG - chr8_2:60856816-60856835 Msa1259290:intron 50.0%
TATTACCGGCAGTACCAGGT+GGG + chr8_2:60856895-60856914 None:intergenic 50.0%
ACCTGGTACTGCCGGTAATA+GGG - chr8_2:60856895-60856914 Msa1259290:intron 50.0%
! GCCGGTAATAGGGAGTTTTG+TGG - chr8_2:60856905-60856924 Msa1259290:intron 50.0%
! TAGGCCCATGGGCTTTGATA+AGG + chr8_2:60856986-60857005 None:intergenic 50.0%
GCCCATGGGCCTAATTAGTT+TGG - chr8_2:60856993-60857012 Msa1259290:intron 50.0%
GCCCAAACTAATTAGGCCCA+TGG + chr8_2:60856998-60857017 None:intergenic 50.0%
ACTGCTCTGTTTCAAGCCCT+TGG - chr8_2:60856282-60856301 Msa1259290:three_prime_UTR 50.0%
TTGTGCGAGACGGAAACAGA+AGG - chr8_2:60856657-60856676 Msa1259290:intron 50.0%
CAAGGTGCAGTGTGCATTCA+AGG - chr8_2:60856816-60856835 Msa1259290:intron 50.0%
TATTACCGGCAGTACCAGGT+GGG + chr8_2:60856895-60856914 None:intergenic 50.0%
ACCTGGTACTGCCGGTAATA+GGG - chr8_2:60856895-60856914 Msa1259290:intron 50.0%
! GCCGGTAATAGGGAGTTTTG+TGG - chr8_2:60856905-60856924 Msa1259290:intron 50.0%
! TAGGCCCATGGGCTTTGATA+AGG + chr8_2:60856986-60857005 None:intergenic 50.0%
GCCCATGGGCCTAATTAGTT+TGG - chr8_2:60856993-60857012 Msa1259290:intron 50.0%
GCCCAAACTAATTAGGCCCA+TGG + chr8_2:60856998-60857017 None:intergenic 50.0%
!! CATGTGGGACTTGCTGTGTG+AGG - chr8_2:60856858-60856877 Msa1259290:CDS 55.0%
CTATTACCGGCAGTACCAGG+TGG + chr8_2:60856896-60856915 None:intergenic 55.0%
CACCTGGTACTGCCGGTAAT+AGG - chr8_2:60856894-60856913 Msa1259290:CDS 55.0%
TCCCTATTACCGGCAGTACC+AGG + chr8_2:60856899-60856918 None:intergenic 55.0%
!! CATGTGGGACTTGCTGTGTG+AGG - chr8_2:60856858-60856877 Msa1259290:CDS 55.0%
CTATTACCGGCAGTACCAGG+TGG + chr8_2:60856896-60856915 None:intergenic 55.0%
CACCTGGTACTGCCGGTAAT+AGG - chr8_2:60856894-60856913 Msa1259290:CDS 55.0%
TCCCTATTACCGGCAGTACC+AGG + chr8_2:60856899-60856918 None:intergenic 55.0%
CAGACTGTGGAGGGCTATGC+AGG - chr8_2:60856792-60856811 Msa1259290:intron 60.0%
GTGGAGGGCTATGCAGGTCA+AGG - chr8_2:60856798-60856817 Msa1259290:intron 60.0%
AGGTGCAAGTGTGTCCCACC+TGG - chr8_2:60856878-60856897 Msa1259290:CDS 60.0%
TGTGTCCCACCTGGTACTGC+CGG - chr8_2:60856887-60856906 Msa1259290:CDS 60.0%
CAGACTGTGGAGGGCTATGC+AGG - chr8_2:60856792-60856811 Msa1259290:intron 60.0%
GTGGAGGGCTATGCAGGTCA+AGG - chr8_2:60856798-60856817 Msa1259290:intron 60.0%
AGGTGCAAGTGTGTCCCACC+TGG - chr8_2:60856878-60856897 Msa1259290:CDS 60.0%
TGTGTCCCACCTGGTACTGC+CGG - chr8_2:60856887-60856906 Msa1259290:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 60856137 60857244 60856137 ID=Msa1259290;Name=Msa1259290
chr8_2 mRNA 60856137 60857244 60856137 ID=Msa1259290-mRNA-1;Parent=Msa1259290;Name=Msa1259290-mRNA-1;_AED=0.08;_eAED=0.08;_QI=39|1|1|1|1|1|4|265|115
chr8_2 exon 60856137 60856586 60856137 ID=Msa1259290-mRNA-1:exon:19091;Parent=Msa1259290-mRNA-1
chr8_2 exon 60856686 60856728 60856686 ID=Msa1259290-mRNA-1:exon:19090;Parent=Msa1259290-mRNA-1
chr8_2 exon 60856851 60856913 60856851 ID=Msa1259290-mRNA-1:exon:19089;Parent=Msa1259290-mRNA-1
chr8_2 exon 60857149 60857244 60857149 ID=Msa1259290-mRNA-1:exon:19088;Parent=Msa1259290-mRNA-1
chr8_2 five_prime_UTR 60857206 60857244 60857206 ID=Msa1259290-mRNA-1:five_prime_utr;Parent=Msa1259290-mRNA-1
chr8_2 CDS 60857149 60857205 60857149 ID=Msa1259290-mRNA-1:cds;Parent=Msa1259290-mRNA-1
chr8_2 CDS 60856851 60856913 60856851 ID=Msa1259290-mRNA-1:cds;Parent=Msa1259290-mRNA-1
chr8_2 CDS 60856686 60856728 60856686 ID=Msa1259290-mRNA-1:cds;Parent=Msa1259290-mRNA-1
chr8_2 CDS 60856402 60856586 60856402 ID=Msa1259290-mRNA-1:cds;Parent=Msa1259290-mRNA-1
chr8_2 three_prime_UTR 60856137 60856401 60856137 ID=Msa1259290-mRNA-1:three_prime_utr;Parent=Msa1259290-mRNA-1
Gene Sequence

>Msa1259290

ATGGCATTACGCAAGCTTCTTGTCTTCGGGTTGTTGCTTTTAGTGTGCCTCTCTAAGGTTTCATCTGATCATGAGATAGAAAAGGAAGAAGATGAAGAAATGCATTTAACTGATAAGCCGCTTATTGTGCGAGACGGAAACAGAAGGCTAATGCAAGACATAGACTGTGGAGGGCTATGCAGGTCAAGGTGCAGTGTGCATTCAAGGCCAAATTTGTGCAAGAGAGCATGTGGGACTTGCTGTGTGAGGTGCAAGTGTGTCCCACCTGGTACTGCCGGTAATAGGGAGTTTTGTGGAGCTTGTTACACTGATATGACCACTCATGGTAACAAGACCAAGTGCCCTTAG
Protein sequence

>Msa1259290

MALRKLLVFGLLLLVCLSKVSSDHEIEKEEDEEMHLTDKPLIVRDGNRRLMQDIDCGGLCRSRCSVHSRPNLCKRACGTCCVRCKCVPPGTAGNREFCGACYTDMTTHGNKTKCP*