AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1210900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1210900 MtrunA17_Chr4g0059421 99.130 115 1 0 1 115 1 115 1.29e-80 231
Msa1210900 MtrunA17_Chr8g0364311 56.364 110 44 1 6 115 7 112 1.52e-38 125
Msa1210900 MtrunA17_Chr3g0142671 47.826 115 48 3 1 115 1 103 4.53e-28 98.6
Msa1210900 MtrunA17_Chr1g0156601 46.957 115 47 3 1 115 1 101 7.41e-27 95.5
Msa1210900 MtrunA17_Chr6g0451481 72.131 61 17 0 55 115 159 219 4.75e-25 94.4
Msa1210900 MtrunA17_Chr1g0156621 63.636 66 23 1 50 115 36 100 7.49e-23 85.1
Msa1210900 MtrunA17_Chr8g0371941 62.121 66 24 1 50 115 39 103 1.17e-21 82.4
Msa1210900 MtrunA17_Chr1g0200531 50.000 76 36 2 41 115 26 100 2.19e-19 76.6
Msa1210900 MtrunA17_Chr6g0451501 57.377 61 26 0 55 115 102 162 3.38e-17 72.4
Msa1210900 MtrunA17_Chr6g0451491 59.677 62 24 1 55 115 69 130 3.67e-17 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1210900 AT5G14920.1 66.129 62 21 0 54 115 214 275 2.04e-22 89.0
Msa1210900 AT4G09600.1 64.516 62 21 1 54 115 39 99 9.39e-22 82.8
Msa1210900 AT2G18420.1 62.903 62 22 1 54 115 34 94 8.00e-21 80.5
Msa1210900 AT2G18420.2 62.903 62 22 1 54 115 10 70 8.04e-21 79.7
Msa1210900 AT1G75750.1 57.576 66 27 1 50 115 34 98 1.09e-20 80.1
Msa1210900 AT1G75750.2 57.576 66 27 1 50 115 33 97 1.27e-20 79.7
Msa1210900 AT1G22690.1 58.065 62 25 1 54 115 59 119 6.00e-19 76.3
Msa1210900 AT1G22690.2 58.065 62 25 1 54 115 59 119 6.00e-19 76.3
Msa1210900 AT1G22690.3 58.065 62 25 1 54 115 59 119 6.00e-19 76.3
Msa1210900 AT4G09610.1 61.290 62 23 1 54 115 39 99 3.67e-13 60.8
Msa1210900 AT1G74670.1 31.858 113 65 2 3 115 1 101 2.44e-11 56.2

Find 48 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTTAATATTGTTGATATT+TGG 0.162273 8_1:-70214965 Msa1210900:three_prime_UTR
CATGCTCTCTTGCACAAATT+TGG 0.199596 8_1:+70215348 None:intergenic
TACTAGTGTAGTTTGGTAAA+TGG 0.244704 8_1:-70215145 Msa1210900:three_prime_UTR
TTTAAAATTCTCCTCTCTAA+TGG 0.280437 8_1:-70216028 Msa1210900:exon
GCCCATGGGCCTAATTAGTT+TGG 0.284816 8_1:-70215194 Msa1210900:three_prime_UTR
GGGCTTTGATAAGGTCTCTA+AGG 0.305310 8_1:+70215213 None:intergenic
TTACGCAAGCTTCTTGTCTT+CGG 0.310911 8_1:-70216003 Msa1210900:CDS
GAAGAGAGCCCAAACTAATT+AGG 0.320634 8_1:+70215185 None:intergenic
TACGCAAGCTTCTTGTCTTC+GGG 0.326759 8_1:-70216002 Msa1210900:CDS
CACCTGGTACTGCCGGTAAT+AGG 0.335185 8_1:-70215293 Msa1210900:CDS
TATGTTGTGGTAACTAACTA+TGG 0.368562 8_1:-70215018 Msa1210900:three_prime_UTR
ACCTGGTACTGCCGGTAATA+GGG 0.401958 8_1:-70215292 Msa1210900:CDS
CCCATGGGCCTAATTAGTTT+GGG 0.408002 8_1:-70215193 Msa1210900:three_prime_UTR
TCCACAAAACTCCCTATTAC+CGG 0.431014 8_1:+70215281 None:intergenic
ACTAGTGTAGTTTGGTAAAT+GGG 0.439046 8_1:-70215144 Msa1210900:three_prime_UTR
TAGGCCCATGGGCTTTGATA+AGG 0.442762 8_1:+70215204 None:intergenic
CAAGGTGCAGTGTGCATTCA+AGG 0.442762 8_1:-70215371 Msa1210900:CDS
CAGACTGTGGAGGGCTATGC+AGG 0.492139 8_1:-70215395 Msa1210900:CDS
TAGTACCTACTAGTGTAGTT+TGG 0.501441 8_1:-70215152 Msa1210900:three_prime_UTR
ATTTACCAAACTACACTAGT+AGG 0.502074 8_1:+70215147 None:intergenic
CTTGGTCTTGTTACCATGAG+TGG 0.515862 8_1:+70215239 None:intergenic
ATCTGATCATGAGATAGAAA+AGG 0.540603 8_1:-70215712 Msa1210900:CDS
TCCCTATTACCGGCAGTACC+AGG 0.543014 8_1:+70215291 None:intergenic
AATGCATTTAACTGATAAGC+CGG 0.550151 8_1:-70215676 Msa1210900:intron
GCCCAAACTAATTAGGCCCA+TGG 0.551582 8_1:+70215192 None:intergenic
ATAAGGTCTCTAAGGGCACT+TGG 0.552905 8_1:+70215221 None:intergenic
TGTGTCCCACCTGGTACTGC+CGG 0.553087 8_1:-70215300 Msa1210900:CDS
GGCTTTGATAAGGTCTCTAA+GGG 0.558100 8_1:+70215214 None:intergenic
CTTCAGCTTATTGTGCGAGA+CGG 0.558872 8_1:-70215540 Msa1210900:intron
AGAGACCTTATCAAAGCCCA+TGG 0.565394 8_1:-70215209 Msa1210900:three_prime_UTR
TACACTGATATGACCACTCA+TGG 0.567522 8_1:-70215252 Msa1210900:CDS
TGTTAAACCTTTGTATTCGT+AGG 0.570058 8_1:-70215065 Msa1210900:three_prime_UTR
CCCAAACTAATTAGGCCCAT+GGG 0.581285 8_1:+70215193 None:intergenic
AGAAGGCTAATGCAAGACAT+AGG 0.585779 8_1:-70215513 Msa1210900:intron
AGGTGCAAGTGTGTCCCACC+TGG 0.590839 8_1:-70215309 Msa1210900:CDS
AATTTGTGCAAGAGAGCATG+TGG 0.594899 8_1:-70215345 Msa1210900:CDS
GTGGAGGGCTATGCAGGTCA+AGG 0.600120 8_1:-70215389 Msa1210900:CDS
TTGTGCGAGACGGAAACAGA+AGG 0.605905 8_1:-70215530 Msa1210900:CDS
TCAAAATCCTACGAATACAA+AGG 0.641840 8_1:+70215058 None:intergenic
CATGTGGGACTTGCTGTGTG+AGG 0.647718 8_1:-70215329 Msa1210900:CDS
TACAAGTTAGTACCTTAGAG+AGG 0.650629 8_1:+70215962 None:intergenic
CTTGCGTAATGCCATTAGAG+AGG 0.657310 8_1:+70216017 None:intergenic
GAGACCTTATCAAAGCCCAT+GGG 0.661282 8_1:-70215208 Msa1210900:three_prime_UTR
TACACTAGTAGGTACTACGT+GGG 0.679336 8_1:+70215158 None:intergenic
CTACACTAGTAGGTACTACG+TGG 0.684236 8_1:+70215157 None:intergenic
CTATTACCGGCAGTACCAGG+TGG 0.706350 8_1:+70215294 None:intergenic
TATTACCGGCAGTACCAGGT+GGG 0.719553 8_1:+70215295 None:intergenic
ATTTGTGCAAGAGAGCATGT+GGG 0.729137 8_1:-70215344 Msa1210900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTATATTTAGTTTTATTTTT+TGG + chr8_1:70215530-70215549 None:intergenic 10.0%
!! TTTGAATGAAAAAAAATAAA+AGG - chr8_1:70215900-70215919 Msa1210900:intron 10.0%
!!! GTATATTTAGTTTTATTTTT+TGG + chr8_1:70215530-70215549 None:intergenic 10.0%
!! TTTGAATGAAAAAAAATAAA+AGG - chr8_1:70215900-70215919 Msa1210900:intron 10.0%
!! GTTCTATTTAAGTATAAAAA+AGG + chr8_1:70215420-70215439 None:intergenic 15.0%
!! ATAAGCATTTAACATATATT+AGG + chr8_1:70215442-70215461 None:intergenic 15.0%
!! GTTCTATTTAAGTATAAAAA+AGG + chr8_1:70215420-70215439 None:intergenic 15.0%
!! ATAAGCATTTAACATATATT+AGG + chr8_1:70215442-70215461 None:intergenic 15.0%
!! TTTCAACCAATAACTTAAAA+TGG + chr8_1:70215159-70215178 None:intergenic 20.0%
!! AGAAAGTAAAAAATGAATAC+CGG + chr8_1:70215359-70215378 None:intergenic 20.0%
!!! TTCATTTTTTACTTTCTTTC+TGG - chr8_1:70215362-70215381 Msa1210900:CDS 20.0%
!! GAATGAAAAAAAATAAAAGG+AGG - chr8_1:70215903-70215922 Msa1210900:intron 20.0%
!! CGTTTAATATTGTTGATATT+TGG - chr8_1:70216048-70216067 Msa1210900:five_prime_UTR 20.0%
!! TTTCAACCAATAACTTAAAA+TGG + chr8_1:70215159-70215178 None:intergenic 20.0%
!! AGAAAGTAAAAAATGAATAC+CGG + chr8_1:70215359-70215378 None:intergenic 20.0%
!!! TTCATTTTTTACTTTCTTTC+TGG - chr8_1:70215362-70215381 Msa1210900:CDS 20.0%
!! GAATGAAAAAAAATAAAAGG+AGG - chr8_1:70215903-70215922 Msa1210900:intron 20.0%
!! TTTAAAATTCTCCTCTCTAA+TGG - chr8_1:70214985-70215004 Msa1210900:three_prime_UTR 25.0%
! AATGTTCAGTATAAGATGAA+AGG + chr8_1:70215081-70215100 None:intergenic 25.0%
!!! TTTGGACCATTTTAAGTTAT+TGG - chr8_1:70215150-70215169 Msa1210900:three_prime_UTR 25.0%
!! ATAAAAAAGGTGAAGTACTT+TGG + chr8_1:70215407-70215426 None:intergenic 25.0%
!! TTTAAAATTCTCCTCTCTAA+TGG - chr8_1:70214985-70215004 Msa1210900:three_prime_UTR 25.0%
! AATGTTCAGTATAAGATGAA+AGG + chr8_1:70215081-70215100 None:intergenic 25.0%
!!! TTTGGACCATTTTAAGTTAT+TGG - chr8_1:70215150-70215169 Msa1210900:three_prime_UTR 25.0%
!! ATAAAAAAGGTGAAGTACTT+TGG + chr8_1:70215407-70215426 None:intergenic 25.0%
ATCTGATCATGAGATAGAAA+AGG - chr8_1:70215301-70215320 Msa1210900:CDS 30.0%
AATGCATTTAACTGATAAGC+CGG - chr8_1:70215337-70215356 Msa1210900:CDS 30.0%
ATTTACCAAACTACACTAGT+AGG + chr8_1:70215869-70215888 None:intergenic 30.0%
TACTAGTGTAGTTTGGTAAA+TGG - chr8_1:70215868-70215887 Msa1210900:intron 30.0%
ACTAGTGTAGTTTGGTAAAT+GGG - chr8_1:70215869-70215888 Msa1210900:intron 30.0%
TGTTAAACCTTTGTATTCGT+AGG - chr8_1:70215948-70215967 Msa1210900:intron 30.0%
TCAAAATCCTACGAATACAA+AGG + chr8_1:70215958-70215977 None:intergenic 30.0%
!! GTAGGATTTTGATGTATTCT+CGG - chr8_1:70215966-70215985 Msa1210900:intron 30.0%
TATGTTGTGGTAACTAACTA+TGG - chr8_1:70215995-70216014 Msa1210900:CDS 30.0%
ATCTGATCATGAGATAGAAA+AGG - chr8_1:70215301-70215320 Msa1210900:CDS 30.0%
AATGCATTTAACTGATAAGC+CGG - chr8_1:70215337-70215356 Msa1210900:CDS 30.0%
ATTTACCAAACTACACTAGT+AGG + chr8_1:70215869-70215888 None:intergenic 30.0%
TACTAGTGTAGTTTGGTAAA+TGG - chr8_1:70215868-70215887 Msa1210900:intron 30.0%
ACTAGTGTAGTTTGGTAAAT+GGG - chr8_1:70215869-70215888 Msa1210900:intron 30.0%
TGTTAAACCTTTGTATTCGT+AGG - chr8_1:70215948-70215967 Msa1210900:intron 30.0%
TCAAAATCCTACGAATACAA+AGG + chr8_1:70215958-70215977 None:intergenic 30.0%
!! GTAGGATTTTGATGTATTCT+CGG - chr8_1:70215966-70215985 Msa1210900:intron 30.0%
TATGTTGTGGTAACTAACTA+TGG - chr8_1:70215995-70216014 Msa1210900:CDS 30.0%
TACAAGTTAGTACCTTAGAG+AGG + chr8_1:70215054-70215073 None:intergenic 35.0%
GTTCAGTATAAGATGAAAGG+TGG + chr8_1:70215078-70215097 None:intergenic 35.0%
! GTGTTTTGTCAATATCATGC+AGG - chr8_1:70215274-70215293 Msa1210900:CDS 35.0%
TAGTACCTACTAGTGTAGTT+TGG - chr8_1:70215861-70215880 Msa1210900:intron 35.0%
! TTCTCGGTTTTTGTATGTTG+TGG - chr8_1:70215982-70216001 Msa1210900:CDS 35.0%
TACAAGTTAGTACCTTAGAG+AGG + chr8_1:70215054-70215073 None:intergenic 35.0%
GTTCAGTATAAGATGAAAGG+TGG + chr8_1:70215078-70215097 None:intergenic 35.0%
! GTGTTTTGTCAATATCATGC+AGG - chr8_1:70215274-70215293 Msa1210900:CDS 35.0%
TAGTACCTACTAGTGTAGTT+TGG - chr8_1:70215861-70215880 Msa1210900:intron 35.0%
! TTCTCGGTTTTTGTATGTTG+TGG - chr8_1:70215982-70216001 Msa1210900:CDS 35.0%
TTACGCAAGCTTCTTGTCTT+CGG - chr8_1:70215010-70215029 Msa1210900:three_prime_UTR 40.0%
AAGCCCTTGGTTTATGTCAT+GGG - chr8_1:70215123-70215142 Msa1210900:three_prime_UTR 40.0%
!! GTTTATGTCATGGGGTGTTT+TGG - chr8_1:70215132-70215151 Msa1210900:three_prime_UTR 40.0%
AGAAGGCTAATGCAAGACAT+AGG - chr8_1:70215500-70215519 Msa1210900:intron 40.0%
! GATCAATTTTGTGCAGACTG+TGG - chr8_1:70215605-70215624 Msa1210900:intron 40.0%
! AATTTTGTGCAGACTGTGGA+GGG - chr8_1:70215609-70215628 Msa1210900:intron 40.0%
CATGCTCTCTTGCACAAATT+TGG + chr8_1:70215668-70215687 None:intergenic 40.0%
! AATTTGTGCAAGAGAGCATG+TGG - chr8_1:70215668-70215687 Msa1210900:intron 40.0%
! ATTTGTGCAAGAGAGCATGT+GGG - chr8_1:70215669-70215688 Msa1210900:intron 40.0%
TCCACAAAACTCCCTATTAC+CGG + chr8_1:70215735-70215754 None:intergenic 40.0%
TACACTGATATGACCACTCA+TGG - chr8_1:70215761-70215780 Msa1210900:intron 40.0%
! GGCTTTGATAAGGTCTCTAA+GGG + chr8_1:70215802-70215821 None:intergenic 40.0%
GAAGAGAGCCCAAACTAATT+AGG + chr8_1:70215831-70215850 None:intergenic 40.0%
TACACTAGTAGGTACTACGT+GGG + chr8_1:70215858-70215877 None:intergenic 40.0%
TTACGCAAGCTTCTTGTCTT+CGG - chr8_1:70215010-70215029 Msa1210900:three_prime_UTR 40.0%
AAGCCCTTGGTTTATGTCAT+GGG - chr8_1:70215123-70215142 Msa1210900:three_prime_UTR 40.0%
!! GTTTATGTCATGGGGTGTTT+TGG - chr8_1:70215132-70215151 Msa1210900:three_prime_UTR 40.0%
AGAAGGCTAATGCAAGACAT+AGG - chr8_1:70215500-70215519 Msa1210900:intron 40.0%
! GATCAATTTTGTGCAGACTG+TGG - chr8_1:70215605-70215624 Msa1210900:intron 40.0%
! AATTTTGTGCAGACTGTGGA+GGG - chr8_1:70215609-70215628 Msa1210900:intron 40.0%
CATGCTCTCTTGCACAAATT+TGG + chr8_1:70215668-70215687 None:intergenic 40.0%
! AATTTGTGCAAGAGAGCATG+TGG - chr8_1:70215668-70215687 Msa1210900:intron 40.0%
! ATTTGTGCAAGAGAGCATGT+GGG - chr8_1:70215669-70215688 Msa1210900:intron 40.0%
TCCACAAAACTCCCTATTAC+CGG + chr8_1:70215735-70215754 None:intergenic 40.0%
TACACTGATATGACCACTCA+TGG - chr8_1:70215761-70215780 Msa1210900:intron 40.0%
! GGCTTTGATAAGGTCTCTAA+GGG + chr8_1:70215802-70215821 None:intergenic 40.0%
GAAGAGAGCCCAAACTAATT+AGG + chr8_1:70215831-70215850 None:intergenic 40.0%
TACACTAGTAGGTACTACGT+GGG + chr8_1:70215858-70215877 None:intergenic 40.0%
CTTGCGTAATGCCATTAGAG+AGG + chr8_1:70214999-70215018 None:intergenic 45.0%
TACGCAAGCTTCTTGTCTTC+GGG - chr8_1:70215011-70215030 Msa1210900:three_prime_UTR 45.0%
! GCTTTTAGTGTGCCTCTCTA+AGG - chr8_1:70215039-70215058 Msa1210900:three_prime_UTR 45.0%
CAAGCCCTTGGTTTATGTCA+TGG - chr8_1:70215122-70215141 Msa1210900:three_prime_UTR 45.0%
AGCCCTTGGTTTATGTCATG+GGG - chr8_1:70215124-70215143 Msa1210900:three_prime_UTR 45.0%
CACCCCATGACATAAACCAA+GGG + chr8_1:70215129-70215148 None:intergenic 45.0%
ACACCCCATGACATAAACCA+AGG + chr8_1:70215130-70215149 None:intergenic 45.0%
CTTCAGCTTATTGTGCGAGA+CGG - chr8_1:70215473-70215492 Msa1210900:intron 45.0%
! CAATTTTGTGCAGACTGTGG+AGG - chr8_1:70215608-70215627 Msa1210900:intron 45.0%
!! CTTGGTCTTGTTACCATGAG+TGG + chr8_1:70215777-70215796 None:intergenic 45.0%
!! ATAAGGTCTCTAAGGGCACT+TGG + chr8_1:70215795-70215814 None:intergenic 45.0%
! GGGCTTTGATAAGGTCTCTA+AGG + chr8_1:70215803-70215822 None:intergenic 45.0%
AGAGACCTTATCAAAGCCCA+TGG - chr8_1:70215804-70215823 Msa1210900:intron 45.0%
GAGACCTTATCAAAGCCCAT+GGG - chr8_1:70215805-70215824 Msa1210900:intron 45.0%
CCCAAACTAATTAGGCCCAT+GGG + chr8_1:70215823-70215842 None:intergenic 45.0%
! CCCATGGGCCTAATTAGTTT+GGG - chr8_1:70215820-70215839 Msa1210900:intron 45.0%
CTACACTAGTAGGTACTACG+TGG + chr8_1:70215859-70215878 None:intergenic 45.0%
CTTGCGTAATGCCATTAGAG+AGG + chr8_1:70214999-70215018 None:intergenic 45.0%
TACGCAAGCTTCTTGTCTTC+GGG - chr8_1:70215011-70215030 Msa1210900:three_prime_UTR 45.0%
! GCTTTTAGTGTGCCTCTCTA+AGG - chr8_1:70215039-70215058 Msa1210900:three_prime_UTR 45.0%
CAAGCCCTTGGTTTATGTCA+TGG - chr8_1:70215122-70215141 Msa1210900:three_prime_UTR 45.0%
AGCCCTTGGTTTATGTCATG+GGG - chr8_1:70215124-70215143 Msa1210900:three_prime_UTR 45.0%
CACCCCATGACATAAACCAA+GGG + chr8_1:70215129-70215148 None:intergenic 45.0%
ACACCCCATGACATAAACCA+AGG + chr8_1:70215130-70215149 None:intergenic 45.0%
CTTCAGCTTATTGTGCGAGA+CGG - chr8_1:70215473-70215492 Msa1210900:intron 45.0%
! CAATTTTGTGCAGACTGTGG+AGG - chr8_1:70215608-70215627 Msa1210900:intron 45.0%
!! CTTGGTCTTGTTACCATGAG+TGG + chr8_1:70215777-70215796 None:intergenic 45.0%
!! ATAAGGTCTCTAAGGGCACT+TGG + chr8_1:70215795-70215814 None:intergenic 45.0%
! GGGCTTTGATAAGGTCTCTA+AGG + chr8_1:70215803-70215822 None:intergenic 45.0%
AGAGACCTTATCAAAGCCCA+TGG - chr8_1:70215804-70215823 Msa1210900:intron 45.0%
GAGACCTTATCAAAGCCCAT+GGG - chr8_1:70215805-70215824 Msa1210900:intron 45.0%
CCCAAACTAATTAGGCCCAT+GGG + chr8_1:70215823-70215842 None:intergenic 45.0%
! CCCATGGGCCTAATTAGTTT+GGG - chr8_1:70215820-70215839 Msa1210900:intron 45.0%
CTACACTAGTAGGTACTACG+TGG + chr8_1:70215859-70215878 None:intergenic 45.0%
ACTGCTCTGTTTCAAGCCCT+TGG - chr8_1:70215110-70215129 Msa1210900:three_prime_UTR 50.0%
TTGTGCGAGACGGAAACAGA+AGG - chr8_1:70215483-70215502 Msa1210900:intron 50.0%
CAAGGTGCAGTGTGCATTCA+AGG - chr8_1:70215642-70215661 Msa1210900:intron 50.0%
TATTACCGGCAGTACCAGGT+GGG + chr8_1:70215721-70215740 None:intergenic 50.0%
ACCTGGTACTGCCGGTAATA+GGG - chr8_1:70215721-70215740 Msa1210900:intron 50.0%
! GCCGGTAATAGGGAGTTTTG+TGG - chr8_1:70215731-70215750 Msa1210900:intron 50.0%
! TAGGCCCATGGGCTTTGATA+AGG + chr8_1:70215812-70215831 None:intergenic 50.0%
GCCCATGGGCCTAATTAGTT+TGG - chr8_1:70215819-70215838 Msa1210900:intron 50.0%
GCCCAAACTAATTAGGCCCA+TGG + chr8_1:70215824-70215843 None:intergenic 50.0%
ACTGCTCTGTTTCAAGCCCT+TGG - chr8_1:70215110-70215129 Msa1210900:three_prime_UTR 50.0%
TTGTGCGAGACGGAAACAGA+AGG - chr8_1:70215483-70215502 Msa1210900:intron 50.0%
CAAGGTGCAGTGTGCATTCA+AGG - chr8_1:70215642-70215661 Msa1210900:intron 50.0%
TATTACCGGCAGTACCAGGT+GGG + chr8_1:70215721-70215740 None:intergenic 50.0%
ACCTGGTACTGCCGGTAATA+GGG - chr8_1:70215721-70215740 Msa1210900:intron 50.0%
! GCCGGTAATAGGGAGTTTTG+TGG - chr8_1:70215731-70215750 Msa1210900:intron 50.0%
! TAGGCCCATGGGCTTTGATA+AGG + chr8_1:70215812-70215831 None:intergenic 50.0%
GCCCATGGGCCTAATTAGTT+TGG - chr8_1:70215819-70215838 Msa1210900:intron 50.0%
GCCCAAACTAATTAGGCCCA+TGG + chr8_1:70215824-70215843 None:intergenic 50.0%
!! CATGTGGGACTTGCTGTGTG+AGG - chr8_1:70215684-70215703 Msa1210900:CDS 55.0%
CTATTACCGGCAGTACCAGG+TGG + chr8_1:70215722-70215741 None:intergenic 55.0%
CACCTGGTACTGCCGGTAAT+AGG - chr8_1:70215720-70215739 Msa1210900:CDS 55.0%
TCCCTATTACCGGCAGTACC+AGG + chr8_1:70215725-70215744 None:intergenic 55.0%
!! CATGTGGGACTTGCTGTGTG+AGG - chr8_1:70215684-70215703 Msa1210900:CDS 55.0%
CTATTACCGGCAGTACCAGG+TGG + chr8_1:70215722-70215741 None:intergenic 55.0%
CACCTGGTACTGCCGGTAAT+AGG - chr8_1:70215720-70215739 Msa1210900:CDS 55.0%
TCCCTATTACCGGCAGTACC+AGG + chr8_1:70215725-70215744 None:intergenic 55.0%
CAGACTGTGGAGGGCTATGC+AGG - chr8_1:70215618-70215637 Msa1210900:intron 60.0%
GTGGAGGGCTATGCAGGTCA+AGG - chr8_1:70215624-70215643 Msa1210900:intron 60.0%
AGGTGCAAGTGTGTCCCACC+TGG - chr8_1:70215704-70215723 Msa1210900:CDS 60.0%
TGTGTCCCACCTGGTACTGC+CGG - chr8_1:70215713-70215732 Msa1210900:CDS 60.0%
CAGACTGTGGAGGGCTATGC+AGG - chr8_1:70215618-70215637 Msa1210900:intron 60.0%
GTGGAGGGCTATGCAGGTCA+AGG - chr8_1:70215624-70215643 Msa1210900:intron 60.0%
AGGTGCAAGTGTGTCCCACC+TGG - chr8_1:70215704-70215723 Msa1210900:CDS 60.0%
TGTGTCCCACCTGGTACTGC+CGG - chr8_1:70215713-70215732 Msa1210900:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 70214965 70216070 70214965 ID=Msa1210900;Name=Msa1210900
chr8_1 mRNA 70214965 70216070 70214965 ID=Msa1210900-mRNA-1;Parent=Msa1210900;Name=Msa1210900-mRNA-1;_AED=0.14;_eAED=0.14;_QI=39|1|1|1|1|1|4|265|115
chr8_1 exon 70214965 70215414 70214965 ID=Msa1210900-mRNA-1:exon:23480;Parent=Msa1210900-mRNA-1
chr8_1 exon 70215514 70215556 70215514 ID=Msa1210900-mRNA-1:exon:23479;Parent=Msa1210900-mRNA-1
chr8_1 exon 70215677 70215739 70215677 ID=Msa1210900-mRNA-1:exon:23478;Parent=Msa1210900-mRNA-1
chr8_1 exon 70215975 70216070 70215975 ID=Msa1210900-mRNA-1:exon:23477;Parent=Msa1210900-mRNA-1
chr8_1 five_prime_UTR 70216032 70216070 70216032 ID=Msa1210900-mRNA-1:five_prime_utr;Parent=Msa1210900-mRNA-1
chr8_1 CDS 70215975 70216031 70215975 ID=Msa1210900-mRNA-1:cds;Parent=Msa1210900-mRNA-1
chr8_1 CDS 70215677 70215739 70215677 ID=Msa1210900-mRNA-1:cds;Parent=Msa1210900-mRNA-1
chr8_1 CDS 70215514 70215556 70215514 ID=Msa1210900-mRNA-1:cds;Parent=Msa1210900-mRNA-1
chr8_1 CDS 70215230 70215414 70215230 ID=Msa1210900-mRNA-1:cds;Parent=Msa1210900-mRNA-1
chr8_1 three_prime_UTR 70214965 70215229 70214965 ID=Msa1210900-mRNA-1:three_prime_utr;Parent=Msa1210900-mRNA-1
Gene Sequence

>Msa1210900

ATGGCATTACGCAAGCTTCTTGTCTTCGGGTTGTTGCTTTTAGTGTGCCTCTCTAAGGTTTCATCTGATCATGAGATAGAAAAGGAAGAAGATGAAGAAATGCATTTAACTGATAAGCCGCTTATTGTGCGAGACGGAAACAGAAGGCTAATGCAAGACATAGACTGTGGAGGGCTATGCAGGTCAAGGTGCAGTGTGCATTCAAGGCCAAATTTGTGCAAGAGAGCATGTGGGACTTGCTGTGTGAGGTGCAAGTGTGTCCCACCTGGTACTGCCGGTAATAGGGAGTTTTGTGGAGCTTGTTACACTGATATGACCACTCATGGTAACAAGACCAAGTGCCCTTAG
Protein sequence

>Msa1210900

MALRKLLVFGLLLLVCLSKVSSDHEIEKEEDEEMHLTDKPLIVRDGNRRLMQDIDCGGLCRSRCSVHSRPNLCKRACGTCCVRCKCVPPGTAGNREFCGACYTDMTTHGNKTKCP*