AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1215830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1215830 MtrunA17_Chr4g0065141 96.615 384 13 0 1 384 1 384 0.0 769
Msa1215830 MtrunA17_Chr3g0138281 58.689 305 123 2 79 383 76 377 9.12e-130 377
Msa1215830 MtrunA17_Chr5g0407161 52.145 303 139 3 80 382 80 376 3.10e-104 312
Msa1215830 MtrunA17_Chr1g0194191 48.308 325 140 6 77 383 71 385 1.03e-98 298
Msa1215830 MtrunA17_Chr3g0144111 50.699 286 114 4 98 383 1 259 8.64e-94 281
Msa1215830 MtrunA17_Chr2g0293811 47.321 336 148 6 62 383 46 366 4.13e-89 273
Msa1215830 MtrunA17_Chr5g0430481 36.330 267 134 7 93 348 131 372 4.02e-45 159
Msa1215830 MtrunA17_Chr1g0171571 32.277 347 177 10 16 348 340 642 1.25e-42 157
Msa1215830 MtrunA17_Chr1g0150451 35.206 267 148 5 87 350 79 323 4.01e-42 150
Msa1215830 MtrunA17_Chr3g0105371 33.582 268 148 6 93 352 95 340 3.74e-41 148
Msa1215830 MtrunA17_Chr4g0068971 48.765 162 74 3 226 383 110 266 3.91e-41 145
Msa1215830 MtrunA17_Chr3g0136711 36.402 239 135 4 115 350 56 280 7.67e-41 146
Msa1215830 MtrunA17_Chr1g0212961 36.100 241 133 5 117 354 119 341 4.82e-40 145
Msa1215830 MtrunA17_Chr3g0105351 32.090 268 152 6 93 352 71 316 7.43e-40 144
Msa1215830 MtrunA17_Chr4g0001761 31.661 319 158 5 84 352 22 330 7.68e-40 145
Msa1215830 MtrunA17_Chr6g0483411 30.357 336 186 12 39 351 9 319 6.70e-37 136
Msa1215830 MtrunA17_Chr3g0126511 33.051 236 141 4 121 353 122 343 1.98e-35 133
Msa1215830 MtrunA17_Chr3g0115851 32.979 282 153 11 79 351 28 282 1.08e-34 129
Msa1215830 MtrunA17_Chr4g0050381 33.077 260 143 9 98 351 43 277 1.77e-34 128
Msa1215830 MtrunA17_Chr2g0279151 33.333 261 141 9 98 351 47 281 1.79e-33 125
Msa1215830 MtrunA17_Chr2g0317021 32.584 267 149 9 92 351 91 333 2.24e-33 127
Msa1215830 MtrunA17_Chr1g0150071 35.602 191 114 2 184 369 158 344 1.77e-32 125
Msa1215830 MtrunA17_Chr5g0426141 32.567 261 143 8 98 351 48 282 2.18e-32 123
Msa1215830 MtrunA17_Chr3g0127091 28.808 302 167 8 82 347 57 346 8.52e-32 124
Msa1215830 MtrunA17_Chr8g0360351 32.780 241 139 6 117 351 59 282 1.81e-31 120
Msa1215830 MtrunA17_Chr3g0135961 29.618 314 159 9 93 353 238 542 1.06e-29 120
Msa1215830 MtrunA17_Chr2g0299981 29.363 361 181 11 55 353 190 538 2.70e-29 119
Msa1215830 MtrunA17_Chr1g0150781 29.885 261 138 6 124 348 290 541 2.81e-29 119
Msa1215830 MtrunA17_Chr4g0070871 29.808 312 159 12 87 352 208 505 9.07e-29 117
Msa1215830 MtrunA17_Chr3g0136811 34.104 173 109 1 184 351 158 330 1.20e-28 114
Msa1215830 MtrunA17_Chr1g0158781 31.579 285 157 7 84 348 111 377 1.42e-28 115
Msa1215830 MtrunA17_Chr4g0051661 30.738 244 139 7 113 350 178 397 2.75e-28 114
Msa1215830 MtrunA17_Chr5g0396731 32.016 253 140 7 83 320 70 305 8.41e-28 112
Msa1215830 MtrunA17_Chr3g0112291 28.788 264 144 7 120 348 119 373 1.24e-25 106
Msa1215830 MtrunA17_Chr8g0389921 28.794 257 143 6 122 353 196 437 1.93e-22 98.6
Msa1215830 MtrunA17_Chr5g0435721 30.093 216 126 4 124 323 223 429 8.92e-22 96.7
Msa1215830 MtrunA17_Chr4g0066371 31.250 272 131 13 119 348 81 338 9.64e-22 95.9
Msa1215830 MtrunA17_Chr2g0286951 27.839 273 139 9 119 348 82 339 3.74e-21 94.4
Msa1215830 MtrunA17_Chr7g0263281 28.622 283 146 9 123 353 70 348 3.21e-20 91.3
Msa1215830 MtrunA17_Chr6g0486661 27.666 347 158 16 112 383 116 444 4.56e-20 92.4
Msa1215830 MtrunA17_Chr1g0153671 30.916 262 134 10 123 358 64 304 1.01e-19 90.1
Msa1215830 MtrunA17_Chr2g0295401 29.085 306 156 13 93 354 69 357 4.42e-19 87.8
Msa1215830 MtrunA17_Chr1g0213351 26.531 294 142 11 123 355 79 359 5.98e-19 87.4
Msa1215830 MtrunA17_Chr8g0371321 26.644 289 142 11 123 352 78 355 3.18e-18 85.5
Msa1215830 MtrunA17_Chr7g0256771 29.796 245 124 11 124 328 70 306 3.48e-17 81.6
Msa1215830 MtrunA17_Chr3g0089721 29.661 236 127 10 115 315 60 291 3.88e-17 82.0
Msa1215830 MtrunA17_Chr4g0067711 26.596 282 152 10 118 354 85 356 5.20e-17 81.6
Msa1215830 MtrunA17_Chr3g0089711 29.237 236 128 10 115 315 60 291 1.01e-16 80.9
Msa1215830 MtrunA17_Chr3g0133801 28.309 272 146 11 116 360 56 305 1.78e-16 80.5
Msa1215830 MtrunA17_Chr7g0250061 25.796 314 153 13 123 374 75 370 2.49e-16 79.7
Msa1215830 MtrunA17_Chr1g0211251 26.573 286 146 11 123 352 80 357 3.15e-16 79.7
Msa1215830 MtrunA17_Chr7g0256751 26.502 283 140 12 106 341 52 313 4.70e-16 79.3
Msa1215830 MtrunA17_Chr1g0181901 30.396 227 114 8 123 311 75 295 5.74e-16 79.0
Msa1215830 MtrunA17_Chr7g0272061 27.347 245 119 8 118 312 85 320 6.31e-16 78.6
Msa1215830 MtrunA17_Chr1g0192421 26.882 279 151 13 118 352 83 352 9.86e-16 77.8
Msa1215830 MtrunA17_Chr1g0209451 27.636 275 153 11 119 367 63 317 5.37e-15 76.3
Msa1215830 MtrunA17_Chr4g0004971 30.088 226 104 8 124 304 78 294 7.53e-15 75.1
Msa1215830 MtrunA17_Chr7g0243571 28.287 251 117 8 115 312 83 323 7.99e-15 75.5
Msa1215830 MtrunA17_Chr7g0271971 29.921 254 133 10 119 348 63 295 9.24e-15 75.5
Msa1215830 MtrunA17_Chr2g0294291 27.059 255 138 8 123 337 74 320 1.21e-14 74.7
Msa1215830 MtrunA17_Chr1g0175381 24.850 334 157 14 99 353 37 355 1.27e-14 74.7
Msa1215830 MtrunA17_Chr4g0063051 30.342 234 133 10 124 341 61 280 4.64e-14 72.8
Msa1215830 MtrunA17_Chr1g0192471 28.854 253 137 9 119 348 63 295 7.83e-14 72.4
Msa1215830 MtrunA17_Chr5g0394181 25.200 250 162 9 120 352 358 599 8.70e-13 69.7
Msa1215830 MtrunA17_Chr4g0063111 26.493 268 145 10 114 344 64 316 2.17e-12 68.6
Msa1215830 MtrunA17_Chr7g0249421 28.516 256 106 10 124 311 96 342 5.50e-12 67.0
Msa1215830 MtrunA17_Chr7g0225501 29.537 281 152 16 121 378 83 340 5.59e-12 67.0
Msa1215830 MtrunA17_Chr7g0238501 24.164 269 159 10 116 341 254 520 3.09e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1215830 AT2G25620.1 55.814 344 133 5 44 383 60 388 2.43e-130 379
Msa1215830 AT2G25620.2 55.814 344 133 5 44 383 60 388 2.43e-130 379
Msa1215830 AT2G33700.1 54.934 304 132 2 80 383 79 377 5.04e-116 343
Msa1215830 AT3G51470.2 50.658 304 139 4 79 382 22 314 3.02e-103 308
Msa1215830 AT3G51470.1 50.658 304 139 4 79 382 66 358 7.51e-103 308
Msa1215830 AT3G62260.1 47.256 328 148 6 72 383 64 382 3.15e-90 277
Msa1215830 AT3G62260.2 47.256 328 148 6 72 383 65 383 3.32e-90 277
Msa1215830 AT1G48040.1 43.296 358 183 7 36 382 32 380 8.96e-87 268
Msa1215830 AT3G62260.3 48.344 302 131 6 98 383 1 293 1.83e-82 254
Msa1215830 AT3G17250.1 40.179 336 180 7 61 383 94 421 5.27e-78 247
Msa1215830 AT5G10740.1 37.778 270 137 6 87 350 35 279 8.16e-46 161
Msa1215830 AT5G10740.2 37.778 270 137 6 87 350 35 279 8.16e-46 161
Msa1215830 AT5G24940.1 37.037 270 139 6 87 350 35 279 9.16e-45 160
Msa1215830 AT4G31750.2 36.777 242 136 4 115 353 55 282 7.57e-41 146
Msa1215830 AT4G31750.1 36.777 242 136 4 115 353 55 282 7.87e-41 146
Msa1215830 AT4G31750.3 36.777 242 136 4 115 353 55 282 7.87e-41 146
Msa1215830 AT1G07160.1 34.962 266 142 6 93 348 130 374 8.98e-41 148
Msa1215830 AT1G67820.2 38.222 225 110 5 93 310 92 294 5.43e-39 144
Msa1215830 AT1G67820.1 38.222 225 110 5 93 310 128 330 8.95e-39 144
Msa1215830 AT2G30020.1 34.717 265 143 6 93 348 147 390 1.78e-38 142
Msa1215830 AT1G78200.5 33.077 260 146 8 98 351 1 238 5.59e-38 137
Msa1215830 AT1G78200.4 33.077 260 146 8 98 351 1 238 5.59e-38 137
Msa1215830 AT1G18030.1 27.945 365 220 9 10 351 5 349 1.62e-37 139
Msa1215830 AT1G43900.1 34.836 244 129 7 117 351 147 369 2.95e-37 139
Msa1215830 AT1G78200.1 33.077 260 146 8 98 351 46 283 4.24e-37 136
Msa1215830 AT1G78200.3 33.077 260 146 8 98 351 46 283 4.24e-37 136
Msa1215830 AT1G78200.2 33.077 260 146 8 98 351 46 283 4.24e-37 136
Msa1215830 AT5G53140.1 32.245 245 145 5 114 351 118 348 1.17e-36 138
Msa1215830 AT5G53140.2 32.245 245 145 5 114 351 118 348 1.17e-36 138
Msa1215830 AT2G40180.1 33.091 275 152 7 93 355 136 390 4.32e-36 136
Msa1215830 AT1G22280.1 32.567 261 143 9 98 351 47 281 1.50e-35 132
Msa1215830 AT2G40860.4 32.746 284 152 9 80 348 289 548 5.24e-35 135
Msa1215830 AT2G40860.1 32.746 284 152 9 80 348 387 646 6.08e-35 136
Msa1215830 AT2G20630.1 32.210 267 143 10 94 351 42 279 2.80e-34 128
Msa1215830 AT2G20630.2 31.955 266 143 10 94 350 42 278 4.34e-34 128
Msa1215830 AT1G22280.3 31.835 267 143 10 98 351 47 287 3.27e-33 125
Msa1215830 AT4G28400.3 33.891 239 129 7 122 351 43 261 3.70e-33 125
Msa1215830 AT4G28400.1 33.891 239 129 7 122 351 65 283 8.31e-33 124
Msa1215830 AT1G34750.2 32.946 258 140 10 98 348 48 279 1.45e-32 124
Msa1215830 AT1G34750.1 32.946 258 140 10 98 348 48 279 1.45e-32 124
Msa1215830 AT1G34750.3 32.946 258 140 10 98 348 48 279 1.45e-32 124
Msa1215830 AT2G25070.1 36.158 177 108 1 184 355 158 334 1.88e-32 125
Msa1215830 AT2G25070.2 36.158 177 108 1 184 355 158 334 1.88e-32 125
Msa1215830 AT4G27800.1 26.765 340 202 7 68 372 43 370 2.71e-31 122
Msa1215830 AT2G34740.1 31.535 241 140 8 117 350 112 334 1.20e-30 120
Msa1215830 AT4G31860.1 34.463 177 111 1 184 355 158 334 1.21e-30 120
Msa1215830 AT3G15260.2 33.195 241 138 6 117 351 66 289 2.43e-30 118
Msa1215830 AT3G15260.1 33.195 241 138 6 117 351 66 289 2.43e-30 118
Msa1215830 AT2G40860.2 31.868 273 147 9 80 337 387 635 3.37e-30 122
Msa1215830 AT4G26080.1 29.114 316 169 8 75 348 118 420 5.19e-29 117
Msa1215830 AT1G18030.2 27.532 316 187 8 10 303 5 300 2.20e-28 113
Msa1215830 AT5G51760.2 29.487 312 158 7 87 348 80 379 4.48e-28 114
Msa1215830 AT5G51760.1 32.143 252 131 5 98 321 121 360 6.97e-28 114
Msa1215830 AT4G27800.2 27.517 298 169 7 68 330 43 328 1.28e-27 111
Msa1215830 AT4G27800.3 27.797 295 166 7 68 327 43 325 1.46e-27 111
Msa1215830 AT4G31860.2 29.167 264 131 4 84 301 22 275 2.14e-27 109
Msa1215830 AT1G17550.1 27.798 277 144 6 124 353 237 504 3.34e-27 113
Msa1215830 AT1G17550.2 27.798 277 144 6 124 353 237 504 3.34e-27 113
Msa1215830 AT2G29380.1 31.518 257 134 9 124 348 112 358 1.66e-26 109
Msa1215830 AT1G07430.1 32.692 208 114 5 124 314 157 355 1.88e-26 110
Msa1215830 AT4G31860.3 40.299 134 80 0 184 317 158 291 2.11e-26 107
Msa1215830 AT5G57050.2 28.165 316 190 9 49 338 33 337 2.51e-26 108
Msa1215830 AT5G57050.1 28.165 316 190 9 49 338 73 377 4.03e-26 108
Msa1215830 AT1G72770.4 27.174 276 146 6 124 353 238 504 4.53e-26 109
Msa1215830 AT1G72770.1 27.174 276 146 6 124 353 238 504 4.53e-26 109
Msa1215830 AT1G72770.3 27.174 276 146 6 124 353 238 504 4.53e-26 109
Msa1215830 AT4G28400.2 35.079 191 99 6 122 303 65 239 3.68e-25 103
Msa1215830 AT2G34740.3 33.505 194 108 7 117 303 108 287 1.67e-24 102
Msa1215830 AT2G34740.2 33.505 194 108 7 117 303 126 305 2.40e-24 102
Msa1215830 AT1G34750.4 33.333 213 113 9 98 303 48 238 3.47e-24 100
Msa1215830 AT3G11410.1 30.556 216 116 5 124 314 137 343 1.29e-23 101
Msa1215830 AT5G59220.1 32.195 205 116 4 124 314 148 343 1.82e-23 101
Msa1215830 AT2G20050.1 29.054 296 138 13 124 361 143 424 5.93e-23 101
Msa1215830 AT2G20050.2 29.054 296 138 13 124 361 143 424 6.26e-23 101
Msa1215830 AT2G40860.3 32.174 230 120 8 80 294 387 595 1.87e-22 99.8
Msa1215830 AT4G08260.1 31.579 228 115 7 123 348 18 206 8.88e-22 92.8
Msa1215830 AT5G02760.1 28.975 283 145 10 123 354 71 348 1.15e-21 95.9
Msa1215830 AT3G16800.2 30.147 272 143 11 118 351 87 349 3.82e-20 91.3
Msa1215830 AT3G16800.1 30.147 272 143 11 118 351 87 349 3.82e-20 91.3
Msa1215830 AT3G16800.3 30.147 272 143 11 118 351 87 349 3.82e-20 91.3
Msa1215830 AT1G47380.1 28.819 288 153 12 121 384 61 320 4.30e-18 85.9
Msa1215830 AT1G09160.2 30.709 254 131 10 119 348 63 295 7.65e-18 85.1
Msa1215830 AT1G09160.1 30.709 254 131 10 119 348 63 295 7.65e-18 85.1
Msa1215830 AT3G27140.1 28.099 242 108 6 123 362 18 195 8.86e-18 82.4
Msa1215830 AT3G51370.1 26.645 304 144 13 123 360 78 368 1.54e-17 84.0
Msa1215830 AT3G51370.3 26.645 304 144 13 123 360 78 368 1.54e-17 84.0
Msa1215830 AT4G32950.1 26.207 290 171 10 93 356 50 322 2.28e-17 82.8
Msa1215830 AT3G16800.4 29.658 263 138 11 127 351 1 254 4.14e-17 80.9
Msa1215830 AT3G16800.6 29.658 263 138 11 127 351 1 254 4.14e-17 80.9
Msa1215830 AT5G57050.3 28.571 238 142 8 49 260 73 308 6.01e-17 81.3
Msa1215830 AT4G38520.2 26.573 286 146 9 123 352 80 357 8.99e-17 81.6
Msa1215830 AT4G38520.1 26.573 286 146 9 123 352 80 357 8.99e-17 81.6
Msa1215830 AT3G12620.2 26.712 292 140 11 123 353 82 360 1.26e-16 81.3
Msa1215830 AT3G12620.1 26.712 292 140 11 123 353 82 360 1.26e-16 81.3
Msa1215830 AT1G22280.2 31.544 149 87 5 98 244 47 182 2.55e-16 77.4
Msa1215830 AT3G05640.2 26.374 273 153 10 118 351 87 350 3.48e-16 79.7
Msa1215830 AT3G05640.1 26.374 273 153 10 118 351 87 350 3.48e-16 79.7
Msa1215830 AT3G05640.3 26.374 273 153 10 118 351 87 350 3.48e-16 79.7
Msa1215830 AT5G66080.1 27.668 253 138 11 123 337 81 326 1.14e-15 78.2
Msa1215830 AT3G55050.1 27.273 286 144 11 123 352 83 360 1.72e-15 77.8
Msa1215830 AT3G55050.2 27.273 286 144 11 123 352 83 360 1.72e-15 77.8
Msa1215830 AT5G59220.2 34.000 150 85 3 124 259 148 297 3.69e-15 75.9
Msa1215830 AT1G72770.2 30.488 164 85 3 124 258 238 401 1.10e-13 72.4
Msa1215830 AT1G72770.5 30.488 164 85 3 124 258 238 401 1.10e-13 72.4
Msa1215830 AT5G19280.1 27.757 263 152 11 109 352 336 579 3.90e-13 71.2
Msa1215830 AT3G16560.1 28.977 176 96 6 162 310 247 420 6.40e-13 70.5
Msa1215830 AT3G16560.4 28.977 176 96 6 162 310 247 420 6.40e-13 70.5
Msa1215830 AT3G16560.2 28.977 176 96 6 162 310 247 420 6.40e-13 70.5
Msa1215830 AT3G16560.3 28.977 176 96 6 162 310 247 420 7.15e-13 70.1
Msa1215830 AT4G32950.2 28.571 231 125 9 93 300 50 263 7.54e-13 68.9
Msa1215830 AT5G26010.1 24.290 317 175 11 81 356 38 330 8.88e-13 69.3
Msa1215830 AT5G01700.1 27.313 227 115 8 124 310 29 245 4.58e-12 67.0
Msa1215830 AT5G01700.2 27.313 227 115 8 124 310 78 294 9.02e-12 66.6
Msa1215830 AT5G27930.3 30.088 226 105 11 118 300 86 301 2.94e-11 64.3
Msa1215830 AT1G79630.6 29.714 175 96 5 156 312 83 248 5.84e-11 63.9
Msa1215830 AT1G79630.3 29.714 175 96 5 156 312 83 248 5.84e-11 63.9
Msa1215830 AT1G79630.4 29.714 175 96 5 156 312 83 248 5.84e-11 63.9
Msa1215830 AT1G79630.5 29.714 175 96 5 156 312 83 248 5.84e-11 63.9
Msa1215830 AT1G79630.2 29.714 175 96 5 156 312 123 288 7.03e-11 63.9
Msa1215830 AT1G79630.7 29.714 175 96 5 156 312 123 288 7.03e-11 63.9

Find 105 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTTGACTGCAATTATATT+TGG 0.135131 8_1:+77256875 Msa1215830:CDS
GAGGACTTGGCAAAGAAATT+TGG 0.243759 8_1:+77256454 Msa1215830:CDS
ATTGAGTCTTCCCTGTCTTC+TGG 0.285045 8_1:+77256845 Msa1215830:CDS
TAATTGGAAGTGATGGAATA+TGG 0.292557 8_1:+77257490 Msa1215830:CDS
TAGATGGCATACACCATAAA+AGG 0.303590 8_1:-77256506 None:intergenic
ATCCTTTCTTTCATGCATAA+AGG 0.303955 8_1:-77257285 None:intergenic
ACACTAACAGGAAATGAATT+AGG 0.317080 8_1:-77255549 None:intergenic
GTCAAACACATCGTTGTATA+TGG 0.324717 8_1:+77258028 Msa1215830:three_prime_UTR
TAGCAAAATCTACAGCGTTT+TGG 0.332140 8_1:-77257527 None:intergenic
GCATAAAGGCCTATGATCTT+TGG 0.333154 8_1:-77257271 None:intergenic
GCAAGTTCCTGAGCTGAAAA+AGG 0.336469 8_1:+77255323 Msa1215830:five_prime_UTR
TAGCCAATGCCGGGGACTGC+AGG 0.348722 8_1:+77257211 Msa1215830:CDS
TGATCAAGATAATAATAATA+TGG 0.349493 8_1:+77255410 Msa1215830:CDS
CAGGAACTTGCAGCTGTTGT+AGG 0.352613 8_1:-77255311 None:intergenic
CAATGTTGCAAGGAAATAAT+AGG 0.358586 8_1:+77257582 Msa1215830:CDS
TTGATAACAGAAGGATTTCA+TGG 0.366492 8_1:+77255439 Msa1215830:CDS
ATCAGAACCACCACCACCTT+TGG 0.374581 8_1:+77257662 Msa1215830:CDS
ATTGATGATGGTTACTTGAA+TGG 0.410247 8_1:+77257333 Msa1215830:CDS
ATTCTCAAAAGCATAGGAAT+AGG 0.413644 8_1:-77258089 None:intergenic
CTATTTATTAAACTTTACTT+AGG 0.429229 8_1:+77258004 Msa1215830:three_prime_UTR
TTCGTCCCTACACTTCGGTC+GGG 0.430679 8_1:+77256376 Msa1215830:CDS
GAATAATCTATCTTTCTAAC+AGG 0.434832 8_1:-77257749 None:intergenic
ATGTGAGTATCTTCCATGTA+AGG 0.437191 8_1:-77256424 None:intergenic
TTCTATTACCCCTCCAGCTC+GGG 0.442074 8_1:-77257244 None:intergenic
TATTCTGTGCAGGTATTTGA+TGG 0.443156 8_1:+77256677 Msa1215830:intron
TCACATATGCATTGAGGACT+TGG 0.445711 8_1:+77256441 Msa1215830:CDS
ATGAATTAGGAGCACTGATC+TGG 0.445790 8_1:-77255536 None:intergenic
ATGCTTCTCCTGACTCTGTT+AGG 0.446213 8_1:-77257696 None:intergenic
CACCACCTTTGGTTGTTGAA+CGG 0.446649 8_1:+77257673 Msa1215830:CDS
CTTTACTTGTAGCCAATGCC+GGG 0.446990 8_1:+77257202 Msa1215830:CDS
AAGGAAGTGAGTGGAAGAAA+TGG 0.449462 8_1:+77257405 Msa1215830:CDS
TCGTCCCTACACTTCGGTCG+GGG 0.449528 8_1:+77256377 Msa1215830:CDS
AGTTCTGGCTCAGCACTTAG+TGG 0.454686 8_1:-77257429 None:intergenic
AGCCCTGCAGTCCCCGGCAT+TGG 0.455154 8_1:-77257214 None:intergenic
TATTGGAGGTCGCCCTTACA+TGG 0.456928 8_1:+77256411 Msa1215830:CDS
TCTTTACTTGTAGCCAATGC+CGG 0.457915 8_1:+77257201 Msa1215830:CDS
TGACGCGGGCTCTAGGCAAC+TGG 0.460183 8_1:+77257367 Msa1215830:CDS
CTAGGCGTGACGCGGGCTCT+AGG 0.463224 8_1:+77257360 Msa1215830:CDS
AATGTCATCAATTTAAGTTC+TGG 0.464919 8_1:-77257444 None:intergenic
CTTGGCGGATTTATTGATGA+TGG 0.469287 8_1:+77257321 Msa1215830:CDS
TAGAATTGTCCAAAGATCAT+AGG 0.470481 8_1:+77257262 Msa1215830:CDS
AACAGTAACAAAGGAGTTGT+TGG 0.481782 8_1:-77255355 None:intergenic
CTAGGCAACTGGCATCTTGA+AGG 0.481839 8_1:+77257378 Msa1215830:CDS
TTGAACGGCCTAACAGAGTC+AGG 0.483856 8_1:+77257688 Msa1215830:CDS
GGCCTTTATGCATGAAAGAA+AGG 0.485159 8_1:+77257283 Msa1215830:CDS
CTATCTTTCCCACTAACAAT+GGG 0.504395 8_1:-77257933 None:intergenic
CAAAACAGCCCTGCAGTCCC+CGG 0.507129 8_1:-77257220 None:intergenic
GTGTAGGGACGAAATTCATC+AGG 0.507331 8_1:-77256366 None:intergenic
TTTCGTCCCTACACTTCGGT+CGG 0.513079 8_1:+77256375 Msa1215830:CDS
AAGCAGTTGTACCAGAAGAC+AGG 0.513476 8_1:-77256856 None:intergenic
CTGGCATCTTGAAGGAATGA+AGG 0.515181 8_1:+77257386 Msa1215830:CDS
TGCTAATCAGTGATCACTGG+TGG 0.521465 8_1:+77257842 Msa1215830:three_prime_UTR
AATTGGAAGTGATGGAATAT+GGG 0.533968 8_1:+77257491 Msa1215830:CDS
TCTATCTTTCCCACTAACAA+TGG 0.544525 8_1:-77257934 None:intergenic
AGGAGCACTGATCTGGTCAC+TGG 0.548106 8_1:-77255529 None:intergenic
AGCCAATGCCGGGGACTGCA+GGG 0.552576 8_1:+77257212 Msa1215830:CDS
TTGTGCTAATCAGTGATCAC+TGG 0.555066 8_1:+77257839 Msa1215830:three_prime_UTR
AGATAATTTAACTGTTGTGA+TGG 0.557874 8_1:+77257629 Msa1215830:CDS
TTGTTCGCGATCATCTGCCG+AGG 0.564331 8_1:+77256726 Msa1215830:CDS
ACTTGCAGCTGTTGTAGGCA+TGG 0.564631 8_1:-77255306 None:intergenic
TCAACAACCAAAGGTGGTGG+TGG 0.568942 8_1:-77257669 None:intergenic
TGGAGAAACTTGATAACAGA+AGG 0.570639 8_1:+77255430 Msa1215830:CDS
TATGCATGAAAGAAAGGATG+AGG 0.570968 8_1:+77257289 Msa1215830:CDS
ATTCTATTACCCCTCCAGCT+CGG 0.573089 8_1:-77257245 None:intergenic
CTTCCCTCTTGAACTCGAGA+AGG 0.574740 8_1:+77256769 Msa1215830:CDS
ATGAGGATCGAGTCTCTTGG+CGG 0.579884 8_1:+77257306 Msa1215830:CDS
CGTCCCTACACTTCGGTCGG+GGG 0.582208 8_1:+77256378 Msa1215830:CDS
GTGCAGGTATTTGATGGACA+TGG 0.586679 8_1:+77256683 Msa1215830:intron
TGACTGCAATTATATTTGGA+CGG 0.586908 8_1:+77256879 Msa1215830:CDS
ACCACCTTCTCGAGTTCAAG+AGG 0.589310 8_1:-77256773 None:intergenic
AGGATGAGGATCGAGTCTCT+TGG 0.591958 8_1:+77257303 Msa1215830:CDS
CATGTTGACAACAGTAACAA+AGG 0.593245 8_1:-77255364 None:intergenic
TGGACAGCTAGGCGTGACGC+GGG 0.593529 8_1:+77257353 Msa1215830:CDS
AACTCGAGAAGGTGGTCACA+AGG 0.594388 8_1:+77256780 Msa1215830:CDS
TGAATTTCGTCCCTACACTT+CGG 0.599296 8_1:+77256371 Msa1215830:CDS
TGTGAGTATCTTCCATGTAA+GGG 0.599872 8_1:-77256423 None:intergenic
CAGGAGAAGCATATCTGCTG+AGG 0.600005 8_1:+77257707 Msa1215830:CDS
CGTTCAACAACCAAAGGTGG+TGG 0.600469 8_1:-77257672 None:intergenic
CCCTCTTGAACTCGAGAAGG+TGG 0.601383 8_1:+77256772 Msa1215830:CDS
AGCAGTTGTACCAGAAGACA+GGG 0.602803 8_1:-77256855 None:intergenic
TGCAAAAGATCACAATGCAA+TGG 0.604015 8_1:-77255462 None:intergenic
ATGGACAGCTAGGCGTGACG+CGG 0.605836 8_1:+77257352 Msa1215830:CDS
GCATCCTCAACAATCACCCT+CGG 0.609381 8_1:-77256743 None:intergenic
GGTTACTTGAATGGACAGCT+AGG 0.611625 8_1:+77257342 Msa1215830:CDS
GAAGGAATGAAGGAAGTGAG+TGG 0.614412 8_1:+77257396 Msa1215830:CDS
TTAAAAGAAAACAAACAGTA+CGG 0.614864 8_1:-77258054 None:intergenic
TTAGCTGGAAAGCATTTGTG+AGG 0.615709 8_1:+77256315 Msa1215830:intron
ACAAAGGAGTTGTTGGCAAG+TGG 0.616465 8_1:-77255348 None:intergenic
AGGAGAAGCATATCTGCTGA+GGG 0.620558 8_1:+77257708 Msa1215830:CDS
GCTAATCAGTGATCACTGGT+GGG 0.621957 8_1:+77257843 Msa1215830:three_prime_UTR
GGGGAGTGGTCTGATATTGG+AGG 0.624136 8_1:+77256397 Msa1215830:CDS
TGTTCGCGATCATCTGCCGA+GGG 0.626852 8_1:+77256727 Msa1215830:CDS
TTAGGCCGTTCAACAACCAA+AGG 0.633433 8_1:-77257678 None:intergenic
AGATACTCACATATGCATTG+AGG 0.642485 8_1:+77256435 Msa1215830:CDS
GGCCGTTCAACAACCAAAGG+TGG 0.643136 8_1:-77257675 None:intergenic
GTCAACATGTGTGTGCAAGA+AGG 0.652909 8_1:+77255378 Msa1215830:exon
TGATGTGAAACAATGTTGCA+AGG 0.658172 8_1:+77257572 Msa1215830:CDS
CCACCTTCTCGAGTTCAAGA+GGG 0.662371 8_1:-77256772 None:intergenic
CTAATCAGTGATCACTGGTG+GGG 0.663545 8_1:+77257844 Msa1215830:three_prime_UTR
TTTACTTGTAGCCAATGCCG+GGG 0.668453 8_1:+77257203 Msa1215830:CDS
AGGTATTTGATGGACATGGA+GGG 0.676243 8_1:+77256687 Msa1215830:intron
TCTGCCGAGGGTGATTGTTG+AGG 0.686396 8_1:+77256739 Msa1215830:CDS
CAGGTATTTGATGGACATGG+AGG 0.705749 8_1:+77256686 Msa1215830:intron
ATAGGCGAAGCAATAAAGCG+AGG 0.718434 8_1:+77257600 Msa1215830:CDS
GTAGAGAAAATAATGCACTG+AGG 0.795756 8_1:+77257866 Msa1215830:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGAAATTATGATTTTTA+AGG + chr8_1:77256102-77256121 Msa1215830:intron 10.0%
!!! AATGAAATTATGATTTTTAA+GGG + chr8_1:77256103-77256122 Msa1215830:intron 10.0%
!! AAAGAATAATAATTTCTAAT+GGG + chr8_1:77257080-77257099 Msa1215830:intron 10.0%
!! AAAATAATGATAATTAAACA+AGG - chr8_1:77257171-77257190 None:intergenic 10.0%
!! TGATCAAGATAATAATAATA+TGG + chr8_1:77255410-77255429 Msa1215830:CDS 15.0%
!!! AGATAAGAATTTAAAACAAA+AGG - chr8_1:77256007-77256026 None:intergenic 15.0%
!!! CTTTTTTCTAAATTTGAAAT+TGG + chr8_1:77256033-77256052 Msa1215830:intron 15.0%
!! TAATCATGATTTAACATTAA+AGG + chr8_1:77256647-77256666 Msa1215830:intron 15.0%
!! GAAAGAATAATAATTTCTAA+TGG + chr8_1:77257079-77257098 Msa1215830:intron 15.0%
!! CTATTTATTAAACTTTACTT+AGG + chr8_1:77258004-77258023 Msa1215830:three_prime_UTR 15.0%
!! TAGCTTATAATATAACATAG+GGG - chr8_1:77255641-77255660 None:intergenic 20.0%
!! CTAGCTTATAATATAACATA+GGG - chr8_1:77255642-77255661 None:intergenic 20.0%
!!! ACTAGCTTATAATATAACAT+AGG - chr8_1:77255643-77255662 None:intergenic 20.0%
!!! GTTATATTATAAGCTAGTTT+TGG + chr8_1:77255645-77255664 Msa1215830:intron 20.0%
!!! TAGTTTTGGATTTTTTAAGA+TGG + chr8_1:77255659-77255678 Msa1215830:intron 20.0%
!! AATGCATAATTAAGCAATTA+AGG - chr8_1:77255963-77255982 None:intergenic 20.0%
!! AATTAACAAAATCAGTTAGA+TGG - chr8_1:77256525-77256544 None:intergenic 20.0%
!! AAAAGAAAATTTATGACAAC+AGG - chr8_1:77256549-77256568 None:intergenic 20.0%
!!! CATTTTTGTGATTATAAGAT+AGG + chr8_1:77256590-77256609 Msa1215830:intron 20.0%
!! TTAAAAGAAAACAAACAGTA+CGG - chr8_1:77258057-77258076 None:intergenic 20.0%
! GAAGAAAAGAAGAACAAAAA+TGG - chr8_1:77255619-77255638 None:intergenic 25.0%
!!! TTTAAGATGGTTTTCCAATA+AGG + chr8_1:77255672-77255691 Msa1215830:intron 25.0%
! AAGTATAATGAAAGTCTAAG+AGG - chr8_1:77255698-77255717 None:intergenic 25.0%
! CTTAGACTTTCATTATACTT+AGG + chr8_1:77255698-77255717 Msa1215830:intron 25.0%
!! TTATGTTATTTATCCAAACC+AGG + chr8_1:77255937-77255956 Msa1215830:intron 25.0%
! ACATATCTAAGAGAATTTCA+AGG - chr8_1:77256137-77256156 None:intergenic 25.0%
! CTAATCACATCTTATATCAA+AGG - chr8_1:77256186-77256205 None:intergenic 25.0%
! GAAAATTGCATTCATAACAA+AGG + chr8_1:77256612-77256631 Msa1215830:intron 25.0%
! GATTTAACATTAAAGGCAAA+TGG + chr8_1:77256654-77256673 Msa1215830:intron 25.0%
!!! ATGGTCTTATGGTTATAAAA+TGG - chr8_1:77256931-77256950 None:intergenic 25.0%
!! ATTTTTATCTGTGTCATGTT+CGG - chr8_1:77257016-77257035 None:intergenic 25.0%
!! GATAAAAATATTGACTGCTA+CGG + chr8_1:77257027-77257046 Msa1215830:intron 25.0%
!! ATAAAAATATTGACTGCTAC+GGG + chr8_1:77257028-77257047 Msa1215830:intron 25.0%
!! TTATCATTATTTTCCTGTAC+AGG + chr8_1:77257178-77257197 Msa1215830:intron 25.0%
!! AATGTCATCAATTTAAGTTC+TGG - chr8_1:77257447-77257466 None:intergenic 25.0%
!!! TTTTTGATAATTGGAAGTGA+TGG + chr8_1:77257483-77257502 Msa1215830:CDS 25.0%
! AGATAATTTAACTGTTGTGA+TGG + chr8_1:77257629-77257648 Msa1215830:CDS 25.0%
! GAATAATCTATCTTTCTAAC+AGG - chr8_1:77257752-77257771 None:intergenic 25.0%
! TTGATAACAGAAGGATTTCA+TGG + chr8_1:77255439-77255458 Msa1215830:CDS 30.0%
ACACTAACAGGAAATGAATT+AGG - chr8_1:77255552-77255571 None:intergenic 30.0%
ACAAAAGAAAGAGAAGATGA+AGG - chr8_1:77255582-77255601 None:intergenic 30.0%
ACAAAAATGGCATAACAAGA+AGG - chr8_1:77255606-77255625 None:intergenic 30.0%
! GATTGACTTATTTGAACCTA+TGG + chr8_1:77255730-77255749 Msa1215830:intron 30.0%
!! TTCATAAGTGTTTTTGCCAT+AGG - chr8_1:77255749-77255768 None:intergenic 30.0%
ATCAGCTGAAAACAACTTAT+AGG - chr8_1:77255809-77255828 None:intergenic 30.0%
ATAATTAAGCAATTAAGGCC+TGG - chr8_1:77255958-77255977 None:intergenic 30.0%
!! ATAAGATGTGATTAGTCTCT+AGG + chr8_1:77256191-77256210 Msa1215830:intron 30.0%
!! TAAGATGTGATTAGTCTCTA+GGG + chr8_1:77256192-77256211 Msa1215830:intron 30.0%
!!! TTTCTGTGCATTATTTTAGC+TGG + chr8_1:77256300-77256319 Msa1215830:intron 30.0%
!! AACGAAGCTATTTCCTTTTA+TGG + chr8_1:77256493-77256512 Msa1215830:CDS 30.0%
GCTTTGACTGCAATTATATT+TGG + chr8_1:77256875-77256894 Msa1215830:CDS 30.0%
TGACTGCAATTATATTTGGA+CGG + chr8_1:77256879-77256898 Msa1215830:CDS 30.0%
TATAAAATGGCAAAGAATGG+TGG - chr8_1:77256918-77256937 None:intergenic 30.0%
GGTTATAAAATGGCAAAGAA+TGG - chr8_1:77256921-77256940 None:intergenic 30.0%
! ATAACCATAAGACCATTTTG+TGG + chr8_1:77256935-77256954 Msa1215830:intron 30.0%
TAGAATTGTCCAAAGATCAT+AGG + chr8_1:77257262-77257281 Msa1215830:CDS 30.0%
ATCCTTTCTTTCATGCATAA+AGG - chr8_1:77257288-77257307 None:intergenic 30.0%
!! ATTGATGATGGTTACTTGAA+TGG + chr8_1:77257333-77257352 Msa1215830:CDS 30.0%
! TAATTGGAAGTGATGGAATA+TGG + chr8_1:77257490-77257509 Msa1215830:CDS 30.0%
! AATTGGAAGTGATGGAATAT+GGG + chr8_1:77257491-77257510 Msa1215830:CDS 30.0%
CAATGTTGCAAGGAAATAAT+AGG + chr8_1:77257582-77257601 Msa1215830:CDS 30.0%
!! ATTGTTTTACCCATTGTTAG+TGG + chr8_1:77257924-77257943 Msa1215830:three_prime_UTR 30.0%
!! TTGTTTTACCCATTGTTAGT+GGG + chr8_1:77257925-77257944 Msa1215830:three_prime_UTR 30.0%
AACAGTAACAAAGGAGTTGT+TGG - chr8_1:77255358-77255377 None:intergenic 35.0%
CATGTTGACAACAGTAACAA+AGG - chr8_1:77255367-77255386 None:intergenic 35.0%
! TGGAGAAACTTGATAACAGA+AGG + chr8_1:77255430-77255449 Msa1215830:CDS 35.0%
TGCAAAAGATCACAATGCAA+TGG - chr8_1:77255465-77255484 None:intergenic 35.0%
! TGATCTTTTGCATGCTCAAA+TGG + chr8_1:77255473-77255492 Msa1215830:CDS 35.0%
GAAGGAAATCAAACACTAAC+AGG - chr8_1:77255564-77255583 None:intergenic 35.0%
! AGCTTGTCAAGATAGCTTTA+TGG + chr8_1:77255836-77255855 Msa1215830:intron 35.0%
TGTGAGTATCTTCCATGTAA+GGG - chr8_1:77256426-77256445 None:intergenic 35.0%
ATGTGAGTATCTTCCATGTA+AGG - chr8_1:77256427-77256446 None:intergenic 35.0%
AGATACTCACATATGCATTG+AGG + chr8_1:77256435-77256454 Msa1215830:CDS 35.0%
TAGATGGCATACACCATAAA+AGG - chr8_1:77256509-77256528 None:intergenic 35.0%
TATTCTGTGCAGGTATTTGA+TGG + chr8_1:77256677-77256696 Msa1215830:intron 35.0%
CAACACGACTATAACTCTAA+TGG - chr8_1:77257057-77257076 None:intergenic 35.0%
CATTAGAGTTATAGTCGTGT+TGG + chr8_1:77257055-77257074 Msa1215830:intron 35.0%
!! ACACATGTCGTTATTTTCCT+AGG + chr8_1:77257125-77257144 Msa1215830:intron 35.0%
!! CACATGTCGTTATTTTCCTA+GGG + chr8_1:77257126-77257145 Msa1215830:intron 35.0%
TATGATTGTATGCATTCCCT+AGG - chr8_1:77257145-77257164 None:intergenic 35.0%
TATGCATGAAAGAAAGGATG+AGG + chr8_1:77257289-77257308 Msa1215830:CDS 35.0%
!! GACGACGAGTTTTTGATAAT+TGG + chr8_1:77257474-77257493 Msa1215830:CDS 35.0%
! TAGCAAAATCTACAGCGTTT+TGG - chr8_1:77257530-77257549 None:intergenic 35.0%
TGATGTGAAACAATGTTGCA+AGG + chr8_1:77257572-77257591 Msa1215830:CDS 35.0%
!!! TCTCTGCAATGCCTTATTTT+TGG + chr8_1:77257773-77257792 Msa1215830:three_prime_UTR 35.0%
ATCTCAGCTCACCAAAAATA+AGG - chr8_1:77257787-77257806 None:intergenic 35.0%
GTAGAGAAAATAATGCACTG+AGG + chr8_1:77257866-77257885 Msa1215830:three_prime_UTR 35.0%
CTATCTTTCCCACTAACAAT+GGG - chr8_1:77257936-77257955 None:intergenic 35.0%
TCTATCTTTCCCACTAACAA+TGG - chr8_1:77257937-77257956 None:intergenic 35.0%
GTCAAACACATCGTTGTATA+TGG + chr8_1:77258028-77258047 Msa1215830:three_prime_UTR 35.0%
!! ATGAATTAGGAGCACTGATC+TGG - chr8_1:77255539-77255558 None:intergenic 40.0%
! GAAAGTCTAAGAGGCCTTAT+TGG - chr8_1:77255689-77255708 None:intergenic 40.0%
TAAGCAATTAAGGCCTGGTT+TGG - chr8_1:77255953-77255972 None:intergenic 40.0%
!! TTAGCTGGAAAGCATTTGTG+AGG + chr8_1:77256315-77256334 Msa1215830:intron 40.0%
TGAATTTCGTCCCTACACTT+CGG + chr8_1:77256371-77256390 Msa1215830:CDS 40.0%
TCACATATGCATTGAGGACT+TGG + chr8_1:77256441-77256460 Msa1215830:CDS 40.0%
GAGGACTTGGCAAAGAAATT+TGG + chr8_1:77256454-77256473 Msa1215830:CDS 40.0%
! AGGTATTTGATGGACATGGA+GGG + chr8_1:77256687-77256706 Msa1215830:intron 40.0%
! CTCTCCACAAAATGGTCTTA+TGG - chr8_1:77256942-77256961 None:intergenic 40.0%
CTACAAGTAAAGACCTGTAC+AGG - chr8_1:77257194-77257213 None:intergenic 40.0%
TCTTTACTTGTAGCCAATGC+CGG + chr8_1:77257201-77257220 Msa1215830:CDS 40.0%
GCATAAAGGCCTATGATCTT+TGG - chr8_1:77257274-77257293 None:intergenic 40.0%
GGCCTTTATGCATGAAAGAA+AGG + chr8_1:77257283-77257302 Msa1215830:CDS 40.0%
!! CTTGGCGGATTTATTGATGA+TGG + chr8_1:77257321-77257340 Msa1215830:CDS 40.0%
AAGGAAGTGAGTGGAAGAAA+TGG + chr8_1:77257405-77257424 Msa1215830:CDS 40.0%
! ACGCTGTAGATTTTGCTAGA+AGG + chr8_1:77257532-77257551 Msa1215830:CDS 40.0%
! CTGTAGATTTTGCTAGAAGG+AGG + chr8_1:77257535-77257554 Msa1215830:CDS 40.0%
TTGTGCTAATCAGTGATCAC+TGG + chr8_1:77257839-77257858 Msa1215830:three_prime_UTR 40.0%
GCAAGTTCCTGAGCTGAAAA+AGG + chr8_1:77255323-77255342 Msa1215830:five_prime_UTR 45.0%
!!! AGTGGTACCTTTTTCAGCTC+AGG - chr8_1:77255333-77255352 None:intergenic 45.0%
ACAAAGGAGTTGTTGGCAAG+TGG - chr8_1:77255351-77255370 None:intergenic 45.0%
GTCAACATGTGTGTGCAAGA+AGG + chr8_1:77255378-77255397 Msa1215830:exon 45.0%
GTGTAGGGACGAAATTCATC+AGG - chr8_1:77256369-77256388 None:intergenic 45.0%
AGGCAAATGGTATTCTGTGC+AGG + chr8_1:77256667-77256686 Msa1215830:intron 45.0%
! GTGCAGGTATTTGATGGACA+TGG + chr8_1:77256683-77256702 Msa1215830:intron 45.0%
! CAGGTATTTGATGGACATGG+AGG + chr8_1:77256686-77256705 Msa1215830:intron 45.0%
!! GGTGGTCACAAGGTCATTTT+TGG + chr8_1:77256790-77256809 Msa1215830:CDS 45.0%
ATTGAGTCTTCCCTGTCTTC+TGG + chr8_1:77256845-77256864 Msa1215830:CDS 45.0%
AGCAGTTGTACCAGAAGACA+GGG - chr8_1:77256858-77256877 None:intergenic 45.0%
AAGCAGTTGTACCAGAAGAC+AGG - chr8_1:77256859-77256878 None:intergenic 45.0%
TTGTGCGACTCTCCACAAAA+TGG - chr8_1:77256950-77256969 None:intergenic 45.0%
GTGTCAGACATCACGTTTAG+AGG - chr8_1:77257109-77257128 None:intergenic 45.0%
CTTTACTTGTAGCCAATGCC+GGG + chr8_1:77257202-77257221 Msa1215830:CDS 45.0%
TTTACTTGTAGCCAATGCCG+GGG + chr8_1:77257203-77257222 Msa1215830:CDS 45.0%
ATTCTATTACCCCTCCAGCT+CGG - chr8_1:77257248-77257267 None:intergenic 45.0%
GGTTACTTGAATGGACAGCT+AGG + chr8_1:77257342-77257361 Msa1215830:CDS 45.0%
CTGGCATCTTGAAGGAATGA+AGG + chr8_1:77257386-77257405 Msa1215830:CDS 45.0%
GAAGGAATGAAGGAAGTGAG+TGG + chr8_1:77257396-77257415 Msa1215830:CDS 45.0%
ATAGGCGAAGCAATAAAGCG+AGG + chr8_1:77257600-77257619 Msa1215830:CDS 45.0%
CACCACCTTTGGTTGTTGAA+CGG + chr8_1:77257673-77257692 Msa1215830:CDS 45.0%
TTAGGCCGTTCAACAACCAA+AGG - chr8_1:77257681-77257700 None:intergenic 45.0%
ATGCTTCTCCTGACTCTGTT+AGG - chr8_1:77257699-77257718 None:intergenic 45.0%
! AGGAGAAGCATATCTGCTGA+GGG + chr8_1:77257708-77257727 Msa1215830:CDS 45.0%
TGCTAATCAGTGATCACTGG+TGG + chr8_1:77257842-77257861 Msa1215830:three_prime_UTR 45.0%
GCTAATCAGTGATCACTGGT+GGG + chr8_1:77257843-77257862 Msa1215830:three_prime_UTR 45.0%
CTAATCAGTGATCACTGGTG+GGG + chr8_1:77257844-77257863 Msa1215830:three_prime_UTR 45.0%
!!! TATACATTTTATTTAAAATT+TGG - chr8_1:77257894-77257913 None:intergenic 5.0%
ACTTGCAGCTGTTGTAGGCA+TGG - chr8_1:77255309-77255328 None:intergenic 50.0%
CAGGAACTTGCAGCTGTTGT+AGG - chr8_1:77255314-77255333 None:intergenic 50.0%
TTTCGTCCCTACACTTCGGT+CGG + chr8_1:77256375-77256394 Msa1215830:CDS 50.0%
TATTGGAGGTCGCCCTTACA+TGG + chr8_1:77256411-77256430 Msa1215830:CDS 50.0%
GCATCCTCAACAATCACCCT+CGG - chr8_1:77256746-77256765 None:intergenic 50.0%
CTTCCCTCTTGAACTCGAGA+AGG + chr8_1:77256769-77256788 Msa1215830:CDS 50.0%
CCACCTTCTCGAGTTCAAGA+GGG - chr8_1:77256775-77256794 None:intergenic 50.0%
ACCACCTTCTCGAGTTCAAG+AGG - chr8_1:77256776-77256795 None:intergenic 50.0%
AACTCGAGAAGGTGGTCACA+AGG + chr8_1:77256780-77256799 Msa1215830:CDS 50.0%
TGTGGAGAGTCGCACAACTT+TGG + chr8_1:77256953-77256972 Msa1215830:intron 50.0%
GTGGAGAGTCGCACAACTTT+GGG + chr8_1:77256954-77256973 Msa1215830:intron 50.0%
!! TGTTCGGTGTCTGTGCATCT+TGG - chr8_1:77257000-77257019 None:intergenic 50.0%
TTCTATTACCCCTCCAGCTC+GGG - chr8_1:77257247-77257266 None:intergenic 50.0%
AGGATGAGGATCGAGTCTCT+TGG + chr8_1:77257303-77257322 Msa1215830:CDS 50.0%
ATGAGGATCGAGTCTCTTGG+CGG + chr8_1:77257306-77257325 Msa1215830:CDS 50.0%
CTAGGCAACTGGCATCTTGA+AGG + chr8_1:77257378-77257397 Msa1215830:CDS 50.0%
!! AGTTCTGGCTCAGCACTTAG+TGG - chr8_1:77257432-77257451 None:intergenic 50.0%
ATCAGAACCACCACCACCTT+TGG + chr8_1:77257662-77257681 Msa1215830:CDS 50.0%
! TCAACAACCAAAGGTGGTGG+TGG - chr8_1:77257672-77257691 None:intergenic 50.0%
CGTTCAACAACCAAAGGTGG+TGG - chr8_1:77257675-77257694 None:intergenic 50.0%
TTGAACGGCCTAACAGAGTC+AGG + chr8_1:77257688-77257707 Msa1215830:CDS 50.0%
! CAGGAGAAGCATATCTGCTG+AGG + chr8_1:77257707-77257726 Msa1215830:CDS 50.0%
!! AGGAGCACTGATCTGGTCAC+TGG - chr8_1:77255532-77255551 None:intergenic 55.0%
TTCGTCCCTACACTTCGGTC+GGG + chr8_1:77256376-77256395 Msa1215830:CDS 55.0%
TCGGGGGAGTGGTCTGATAT+TGG + chr8_1:77256394-77256413 Msa1215830:CDS 55.0%
GGGGAGTGGTCTGATATTGG+AGG + chr8_1:77256397-77256416 Msa1215830:CDS 55.0%
TTGTTCGCGATCATCTGCCG+AGG + chr8_1:77256726-77256745 Msa1215830:CDS 55.0%
TGTTCGCGATCATCTGCCGA+GGG + chr8_1:77256727-77256746 Msa1215830:CDS 55.0%
TCTGCCGAGGGTGATTGTTG+AGG + chr8_1:77256739-77256758 Msa1215830:CDS 55.0%
CCCTCTTGAACTCGAGAAGG+TGG + chr8_1:77256772-77256791 Msa1215830:CDS 55.0%
GAGTCGCACAACTTTGGGTC+AGG + chr8_1:77256959-77256978 Msa1215830:intron 55.0%
GGTGTCTGTGCATCTTGGCT+AGG - chr8_1:77256995-77257014 None:intergenic 55.0%
! CTGTTTTGTCCCGAGCTGGA+GGG + chr8_1:77257235-77257254 Msa1215830:CDS 55.0%
! TGTTTTGTCCCGAGCTGGAG+GGG + chr8_1:77257236-77257255 Msa1215830:CDS 55.0%
GGCCGTTCAACAACCAAAGG+TGG - chr8_1:77257678-77257697 None:intergenic 55.0%
TCGTCCCTACACTTCGGTCG+GGG + chr8_1:77256377-77256396 Msa1215830:CDS 60.0%
ACTCCCCCGACCGAAGTGTA+GGG - chr8_1:77256384-77256403 None:intergenic 60.0%
CAAAACAGCCCTGCAGTCCC+CGG - chr8_1:77257223-77257242 None:intergenic 60.0%
!! AGGGCTGTTTTGTCCCGAGC+TGG + chr8_1:77257231-77257250 Msa1215830:CDS 60.0%
!! GCTGTTTTGTCCCGAGCTGG+AGG + chr8_1:77257234-77257253 Msa1215830:CDS 60.0%
ATGGACAGCTAGGCGTGACG+CGG + chr8_1:77257352-77257371 Msa1215830:CDS 60.0%
CGTCCCTACACTTCGGTCGG+GGG + chr8_1:77256378-77256397 Msa1215830:CDS 65.0%
CACTCCCCCGACCGAAGTGT+AGG - chr8_1:77256385-77256404 None:intergenic 65.0%
CTACACTTCGGTCGGGGGAG+TGG + chr8_1:77256383-77256402 Msa1215830:CDS 65.0%
TAGCCAATGCCGGGGACTGC+AGG + chr8_1:77257211-77257230 Msa1215830:CDS 65.0%
AGCCAATGCCGGGGACTGCA+GGG + chr8_1:77257212-77257231 Msa1215830:CDS 65.0%
TGGACAGCTAGGCGTGACGC+GGG + chr8_1:77257353-77257372 Msa1215830:CDS 65.0%
!! TGACGCGGGCTCTAGGCAAC+TGG + chr8_1:77257367-77257386 Msa1215830:CDS 65.0%
AGCCCTGCAGTCCCCGGCAT+TGG - chr8_1:77257217-77257236 None:intergenic 70.0%
CTAGGCGTGACGCGGGCTCT+AGG + chr8_1:77257360-77257379 Msa1215830:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 77255165 77258098 77255165 ID=Msa1215830;Name=Msa1215830
chr8_1 mRNA 77255165 77258098 77255165 ID=Msa1215830-mRNA-1;Parent=Msa1215830;Name=Msa1215830-mRNA-1;_AED=0.05;_eAED=0.05;_QI=219|1|1|1|1|1|4|334|384
chr8_1 exon 77255165 77255563 77255165 ID=Msa1215830-mRNA-1:exon:26615;Parent=Msa1215830-mRNA-1
chr8_1 exon 77256319 77256516 77256319 ID=Msa1215830-mRNA-1:exon:26616;Parent=Msa1215830-mRNA-1
chr8_1 exon 77256689 77256900 77256689 ID=Msa1215830-mRNA-1:exon:26617;Parent=Msa1215830-mRNA-1
chr8_1 exon 77257200 77258098 77257200 ID=Msa1215830-mRNA-1:exon:26618;Parent=Msa1215830-mRNA-1
chr8_1 five_prime_UTR 77255165 77255383 77255165 ID=Msa1215830-mRNA-1:five_prime_utr;Parent=Msa1215830-mRNA-1
chr8_1 CDS 77255384 77255563 77255384 ID=Msa1215830-mRNA-1:cds;Parent=Msa1215830-mRNA-1
chr8_1 CDS 77256319 77256516 77256319 ID=Msa1215830-mRNA-1:cds;Parent=Msa1215830-mRNA-1
chr8_1 CDS 77256689 77256900 77256689 ID=Msa1215830-mRNA-1:cds;Parent=Msa1215830-mRNA-1
chr8_1 CDS 77257200 77257764 77257200 ID=Msa1215830-mRNA-1:cds;Parent=Msa1215830-mRNA-1
chr8_1 three_prime_UTR 77257765 77258098 77257765 ID=Msa1215830-mRNA-1:three_prime_utr;Parent=Msa1215830-mRNA-1
Gene Sequence

>Msa1215830

ATGTGTGTGCAAGAAGGTGAACAAGTTGATCAAGATAATAATAATATGGAGAAACTTGATAACAGAAGGATTTCATGGCCATTGCATTGTGATCTTTTGCATGCTCAAATGGATAAATCAGAAAGAGACTCTTCTATTAGAATTTCCAGTGACCAGATCAGTGCTCCTAATTCATTTCCTCTGGAAAGCATTTGTGAGGATGCAGTGATTGCTGATAAAAAACAGAACCTGATGAATTTCGTCCCTACACTTCGGTCGGGGGAGTGGTCTGATATTGGAGGTCGCCCTTACATGGAAGATACTCACATATGCATTGAGGACTTGGCAAAGAAATTTGGTTACGATGTTGTCTGCAACGAAGCTATTTCCTTTTATGGTGTATTTGATGGACATGGAGGGAAGAGTGCTGCGCAATTTGTTCGCGATCATCTGCCGAGGGTGATTGTTGAGGATGCTGACTTCCCTCTTGAACTCGAGAAGGTGGTCACAAGGTCATTTTTGGAGACTGATTCAGAGTTTGCAAAAACATGCTCTATTGAGTCTTCCCTGTCTTCTGGTACAACTGCTTTGACTGCAATTATATTTGGACGGTCTTTACTTGTAGCCAATGCCGGGGACTGCAGGGCTGTTTTGTCCCGAGCTGGAGGGGTAATAGAATTGTCCAAAGATCATAGGCCTTTATGCATGAAAGAAAGGATGAGGATCGAGTCTCTTGGCGGATTTATTGATGATGGTTACTTGAATGGACAGCTAGGCGTGACGCGGGCTCTAGGCAACTGGCATCTTGAAGGAATGAAGGAAGTGAGTGGAAGAAATGGACCACTAAGTGCTGAGCCAGAACTTAAATTGATGACATTGACAAAAGACGACGAGTTTTTGATAATTGGAAGTGATGGAATATGGGATGTTTTTCGTAGCCAAAACGCTGTAGATTTTGCTAGAAGGAGGCTTCAAGAGCACAATGATGTGAAACAATGTTGCAAGGAAATAATAGGCGAAGCAATAAAGCGAGGAGCAACAGATAATTTAACTGTTGTGATGGTATGTTTTCAATCAGAACCACCACCACCTTTGGTTGTTGAACGGCCTAACAGAGTCAGGAGAAGCATATCTGCTGAGGGACTTCAGAATCTTAAATACCTGTTAGAAAGATAG
Protein sequence

>Msa1215830

MCVQEGEQVDQDNNNMEKLDNRRISWPLHCDLLHAQMDKSERDSSIRISSDQISAPNSFPLESICEDAVIADKKQNLMNFVPTLRSGEWSDIGGRPYMEDTHICIEDLAKKFGYDVVCNEAISFYGVFDGHGGKSAAQFVRDHLPRVIVEDADFPLELEKVVTRSFLETDSEFAKTCSIESSLSSGTTALTAIIFGRSLLVANAGDCRAVLSRAGGVIELSKDHRPLCMKERMRIESLGGFIDDGYLNGQLGVTRALGNWHLEGMKEVSGRNGPLSAEPELKLMTLTKDDEFLIIGSDGIWDVFRSQNAVDFARRRLQEHNDVKQCCKEIIGEAIKRGATDNLTVVMVCFQSEPPPPLVVERPNRVRRSISAEGLQNLKYLLER*