AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1301650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1301650 MtrunA17_Chr4g0065141 97.396 384 10 0 1 384 1 384 0.0 774
Msa1301650 MtrunA17_Chr3g0138281 59.016 305 122 2 79 383 76 377 2.36e-130 379
Msa1301650 MtrunA17_Chr5g0407161 52.145 303 139 3 80 382 80 376 2.84e-104 312
Msa1301650 MtrunA17_Chr1g0194191 48.308 325 140 6 77 383 71 385 1.63e-98 298
Msa1301650 MtrunA17_Chr3g0144111 50.699 286 114 4 98 383 1 259 9.23e-94 281
Msa1301650 MtrunA17_Chr2g0293811 47.321 336 148 6 62 383 46 366 5.07e-89 273
Msa1301650 MtrunA17_Chr5g0430481 36.330 267 134 7 93 348 131 372 2.15e-45 160
Msa1301650 MtrunA17_Chr1g0171571 31.792 346 180 9 16 348 340 642 7.12e-43 158
Msa1301650 MtrunA17_Chr1g0150451 35.206 267 148 5 87 350 79 323 7.20e-42 149
Msa1301650 MtrunA17_Chr3g0105371 33.582 268 148 6 93 352 95 340 3.51e-41 148
Msa1301650 MtrunA17_Chr4g0068971 48.765 162 74 3 226 383 110 266 4.25e-41 145
Msa1301650 MtrunA17_Chr3g0136711 36.402 239 135 4 115 350 56 280 9.86e-41 145
Msa1301650 MtrunA17_Chr4g0001761 31.975 319 157 5 84 352 22 330 1.73e-40 147
Msa1301650 MtrunA17_Chr1g0212961 36.134 238 131 5 120 354 122 341 7.94e-40 144
Msa1301650 MtrunA17_Chr3g0105351 32.090 268 152 6 93 352 71 316 8.07e-40 144
Msa1301650 MtrunA17_Chr6g0483411 30.061 326 183 11 49 351 16 319 7.28e-37 136
Msa1301650 MtrunA17_Chr3g0115851 33.333 282 152 11 79 351 28 282 2.32e-35 131
Msa1301650 MtrunA17_Chr3g0126511 33.051 236 141 4 121 353 122 343 2.89e-35 132
Msa1301650 MtrunA17_Chr4g0050381 33.077 260 143 9 98 351 43 277 2.65e-34 128
Msa1301650 MtrunA17_Chr2g0279151 33.333 261 141 9 98 351 47 281 2.52e-33 125
Msa1301650 MtrunA17_Chr2g0317021 32.210 267 150 9 92 351 91 333 1.06e-32 125
Msa1301650 MtrunA17_Chr1g0150071 35.602 191 114 2 184 369 158 344 1.92e-32 125
Msa1301650 MtrunA17_Chr5g0426141 32.567 261 143 8 98 351 48 282 3.36e-32 122
Msa1301650 MtrunA17_Chr8g0360351 33.195 241 138 6 117 351 59 282 5.02e-32 122
Msa1301650 MtrunA17_Chr3g0127091 28.808 302 167 8 82 347 57 346 1.24e-31 123
Msa1301650 MtrunA17_Chr3g0135961 29.618 314 159 9 93 353 238 542 1.30e-29 120
Msa1301650 MtrunA17_Chr2g0299981 29.006 362 181 11 55 353 190 538 1.52e-29 119
Msa1301650 MtrunA17_Chr1g0150781 29.885 261 138 6 124 348 290 541 2.39e-29 119
Msa1301650 MtrunA17_Chr4g0070871 29.167 312 161 11 87 352 208 505 2.92e-29 118
Msa1301650 MtrunA17_Chr3g0136811 34.104 173 109 1 184 351 158 330 1.38e-28 114
Msa1301650 MtrunA17_Chr1g0158781 31.579 285 157 7 84 348 111 377 2.18e-28 114
Msa1301650 MtrunA17_Chr4g0051661 30.738 244 139 7 113 350 178 397 2.83e-28 114
Msa1301650 MtrunA17_Chr5g0396731 31.343 268 142 8 78 320 55 305 7.16e-28 112
Msa1301650 MtrunA17_Chr3g0112291 29.167 264 143 8 120 348 119 373 1.37e-25 106
Msa1301650 MtrunA17_Chr8g0389921 28.794 257 143 6 122 353 196 437 2.32e-22 98.2
Msa1301650 MtrunA17_Chr5g0435721 30.093 216 126 4 124 323 223 429 1.13e-21 96.3
Msa1301650 MtrunA17_Chr4g0066371 30.855 269 130 12 122 348 84 338 2.66e-21 94.7
Msa1301650 MtrunA17_Chr2g0286951 27.473 273 140 9 119 348 82 339 1.76e-20 92.4
Msa1301650 MtrunA17_Chr7g0263281 28.622 283 146 9 123 353 70 348 3.50e-20 90.9
Msa1301650 MtrunA17_Chr6g0486661 27.378 347 159 15 112 383 116 444 7.27e-20 91.7
Msa1301650 MtrunA17_Chr1g0153671 30.916 262 134 10 123 358 64 304 1.27e-19 90.1
Msa1301650 MtrunA17_Chr1g0213351 26.531 294 142 11 123 355 79 359 5.34e-19 87.8
Msa1301650 MtrunA17_Chr2g0295401 29.085 306 156 13 93 354 69 357 5.71e-19 87.4
Msa1301650 MtrunA17_Chr8g0371321 26.644 289 142 11 123 352 78 355 3.43e-18 85.5
Msa1301650 MtrunA17_Chr7g0256771 29.796 245 124 11 124 328 70 306 3.19e-17 81.6
Msa1301650 MtrunA17_Chr3g0133801 28.676 272 145 11 116 360 56 305 4.90e-17 82.4
Msa1301650 MtrunA17_Chr4g0067711 26.596 282 152 10 118 354 85 356 6.46e-17 81.3
Msa1301650 MtrunA17_Chr3g0089721 29.237 236 128 10 115 315 60 291 1.24e-16 80.5
Msa1301650 MtrunA17_Chr7g0250061 25.796 314 153 13 123 374 75 370 2.71e-16 79.7
Msa1301650 MtrunA17_Chr3g0089711 28.814 236 129 10 115 315 60 291 3.38e-16 79.3
Msa1301650 MtrunA17_Chr1g0211251 26.573 286 146 11 123 352 80 357 3.42e-16 79.7
Msa1301650 MtrunA17_Chr7g0256751 26.502 283 140 12 106 341 52 313 5.25e-16 79.3
Msa1301650 MtrunA17_Chr1g0181901 30.396 227 114 8 123 311 75 295 6.18e-16 79.0
Msa1301650 MtrunA17_Chr7g0272061 27.347 245 119 8 118 312 85 320 7.66e-16 78.2
Msa1301650 MtrunA17_Chr1g0192421 26.882 279 151 13 118 352 83 352 1.35e-15 77.4
Msa1301650 MtrunA17_Chr1g0209451 27.839 273 151 11 121 367 65 317 5.47e-15 76.3
Msa1301650 MtrunA17_Chr7g0243571 28.287 251 117 8 115 312 83 323 6.60e-15 75.9
Msa1301650 MtrunA17_Chr4g0004971 30.088 226 104 8 124 304 78 294 8.82e-15 75.1
Msa1301650 MtrunA17_Chr7g0271971 30.159 252 131 10 121 348 65 295 1.10e-14 75.1
Msa1301650 MtrunA17_Chr2g0294291 27.092 251 143 9 123 337 74 320 1.24e-14 74.7
Msa1301650 MtrunA17_Chr1g0175381 25.000 292 142 11 124 353 79 355 2.38e-14 73.9
Msa1301650 MtrunA17_Chr4g0063051 30.453 243 138 11 115 341 53 280 2.79e-14 73.2
Msa1301650 MtrunA17_Chr1g0192471 29.084 251 135 9 121 348 65 295 9.48e-14 72.4
Msa1301650 MtrunA17_Chr5g0394181 25.200 250 162 9 120 352 358 599 8.39e-13 69.7
Msa1301650 MtrunA17_Chr4g0063111 26.744 258 137 10 124 344 74 316 2.35e-12 68.2
Msa1301650 MtrunA17_Chr7g0249421 28.516 256 106 10 124 311 96 342 5.75e-12 67.0
Msa1301650 MtrunA17_Chr7g0225501 29.537 281 152 16 121 378 83 340 6.34e-12 66.6
Msa1301650 MtrunA17_Chr7g0238501 24.535 269 158 10 116 341 254 520 8.34e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1301650 AT2G25620.1 55.556 342 137 3 44 383 60 388 4.51e-130 379
Msa1301650 AT2G25620.2 55.556 342 137 3 44 383 60 388 4.51e-130 379
Msa1301650 AT2G33700.1 54.934 304 132 2 80 383 79 377 1.07e-115 342
Msa1301650 AT3G51470.2 50.658 304 139 4 79 382 22 314 5.86e-103 307
Msa1301650 AT3G51470.1 50.658 304 139 4 79 382 66 358 1.22e-102 308
Msa1301650 AT3G62260.2 47.256 328 148 6 72 383 65 383 4.40e-90 276
Msa1301650 AT3G62260.1 47.256 328 148 6 72 383 64 382 4.80e-90 276
Msa1301650 AT1G48040.1 46.667 315 151 6 79 382 72 380 1.29e-86 268
Msa1301650 AT3G62260.3 48.344 302 131 6 98 383 1 293 2.32e-82 254
Msa1301650 AT3G17250.1 40.179 336 180 7 61 383 94 421 6.60e-78 246
Msa1301650 AT5G10740.1 37.778 270 137 6 87 350 35 279 1.20e-45 160
Msa1301650 AT5G10740.2 37.778 270 137 6 87 350 35 279 1.20e-45 160
Msa1301650 AT5G24940.1 37.037 270 139 6 87 350 35 279 1.55e-44 160
Msa1301650 AT1G07160.1 35.338 266 141 6 93 348 130 374 2.08e-41 150
Msa1301650 AT4G31750.2 36.777 242 136 4 115 353 55 282 1.15e-40 146
Msa1301650 AT4G31750.1 36.777 242 136 4 115 353 55 282 1.62e-40 145
Msa1301650 AT4G31750.3 36.777 242 136 4 115 353 55 282 1.62e-40 145
Msa1301650 AT2G30020.1 35.094 265 142 6 93 348 147 390 4.87e-39 144
Msa1301650 AT1G67820.2 37.778 225 111 5 93 310 92 294 2.51e-38 142
Msa1301650 AT1G67820.1 37.778 225 111 5 93 310 128 330 4.58e-38 142
Msa1301650 AT1G78200.5 31.538 260 150 6 98 351 1 238 2.42e-37 135
Msa1301650 AT1G78200.4 31.538 260 150 6 98 351 1 238 2.42e-37 135
Msa1301650 AT1G18030.1 27.671 365 221 9 10 351 5 349 3.59e-37 138
Msa1301650 AT1G43900.1 32.862 283 157 9 78 351 111 369 3.66e-37 138
Msa1301650 AT1G78200.1 32.308 260 148 7 98 351 46 283 1.20e-36 135
Msa1301650 AT1G78200.3 32.308 260 148 7 98 351 46 283 1.20e-36 135
Msa1301650 AT1G78200.2 32.308 260 148 7 98 351 46 283 1.20e-36 135
Msa1301650 AT5G53140.1 32.766 235 141 4 120 351 128 348 1.74e-36 137
Msa1301650 AT5G53140.2 32.766 235 141 4 120 351 128 348 1.74e-36 137
Msa1301650 AT1G22280.1 32.950 261 142 9 98 351 47 281 3.42e-36 133
Msa1301650 AT2G40180.1 33.091 275 152 7 93 355 136 390 4.23e-36 136
Msa1301650 AT2G40860.4 32.155 283 155 8 80 348 289 548 1.02e-34 135
Msa1301650 AT2G40860.1 32.155 283 155 8 80 348 387 646 1.19e-34 135
Msa1301650 AT2G20630.1 32.210 267 143 10 94 351 42 279 2.95e-34 128
Msa1301650 AT2G20630.2 31.955 266 143 10 94 350 42 278 5.18e-34 128
Msa1301650 AT1G22280.3 32.210 267 142 10 98 351 47 287 8.37e-34 127
Msa1301650 AT4G28400.3 33.891 239 129 7 122 351 43 261 4.14e-33 125
Msa1301650 AT4G28400.1 33.891 239 129 7 122 351 65 283 1.02e-32 124
Msa1301650 AT2G25070.1 36.158 177 108 1 184 355 158 334 2.11e-32 125
Msa1301650 AT2G25070.2 36.158 177 108 1 184 355 158 334 2.11e-32 125
Msa1301650 AT1G34750.2 31.439 264 137 8 111 348 34 279 3.47e-32 123
Msa1301650 AT1G34750.1 31.439 264 137 8 111 348 34 279 3.47e-32 123
Msa1301650 AT1G34750.3 31.439 264 137 8 111 348 34 279 3.47e-32 123
Msa1301650 AT4G27800.1 26.765 340 202 7 68 372 43 370 2.88e-31 122
Msa1301650 AT3G15260.2 33.610 241 137 6 117 351 66 289 8.04e-31 119
Msa1301650 AT3G15260.1 33.610 241 137 6 117 351 66 289 8.04e-31 119
Msa1301650 AT4G31860.1 34.463 177 111 1 184 355 158 334 1.19e-30 120
Msa1301650 AT2G34740.1 31.780 236 136 8 122 350 117 334 1.62e-30 119
Msa1301650 AT2G40860.2 31.250 272 150 8 80 337 387 635 7.13e-30 121
Msa1301650 AT4G26080.1 29.114 316 169 8 75 348 118 420 6.32e-29 117
Msa1301650 AT1G18030.2 27.215 316 188 8 10 303 5 300 4.81e-28 112
Msa1301650 AT4G31860.2 29.545 264 130 4 84 301 22 275 5.27e-28 111
Msa1301650 AT5G51760.2 29.450 309 156 7 90 348 83 379 5.47e-28 114
Msa1301650 AT5G51760.1 31.939 263 139 5 87 321 110 360 8.33e-28 113
Msa1301650 AT4G27800.2 27.517 298 169 7 68 330 43 328 1.65e-27 111
Msa1301650 AT4G27800.3 27.797 295 166 7 68 327 43 325 2.03e-27 111
Msa1301650 AT1G17550.1 27.798 277 144 6 124 353 237 504 3.28e-27 113
Msa1301650 AT1G17550.2 27.798 277 144 6 124 353 237 504 3.28e-27 113
Msa1301650 AT2G29380.1 31.518 257 134 9 124 348 112 358 2.11e-26 108
Msa1301650 AT1G07430.1 32.692 208 114 5 124 314 157 355 2.24e-26 110
Msa1301650 AT4G31860.3 40.299 134 80 0 184 317 158 291 2.38e-26 107
Msa1301650 AT5G57050.2 27.532 316 192 8 49 338 33 337 4.49e-26 108
Msa1301650 AT1G72770.4 27.174 276 146 6 124 353 238 504 4.84e-26 109
Msa1301650 AT1G72770.1 27.174 276 146 6 124 353 238 504 4.84e-26 109
Msa1301650 AT1G72770.3 27.174 276 146 6 124 353 238 504 4.84e-26 109
Msa1301650 AT5G57050.1 30.568 229 134 5 124 338 160 377 6.76e-26 108
Msa1301650 AT4G28400.2 35.079 191 99 6 122 303 65 239 4.03e-25 103
Msa1301650 AT2G34740.3 33.862 189 104 7 122 303 113 287 2.23e-24 102
Msa1301650 AT2G34740.2 33.862 189 104 7 122 303 131 305 2.81e-24 102
Msa1301650 AT1G34750.4 31.507 219 110 7 111 303 34 238 8.33e-24 99.4
Msa1301650 AT3G11410.1 30.556 216 116 5 124 314 137 343 1.37e-23 101
Msa1301650 AT5G59220.1 32.057 209 119 4 124 318 148 347 1.91e-23 101
Msa1301650 AT2G20050.2 29.054 296 138 13 124 361 143 424 7.25e-23 101
Msa1301650 AT2G20050.1 29.054 296 138 13 124 361 143 424 8.03e-23 101
Msa1301650 AT2G40860.3 31.441 229 123 7 80 294 387 595 3.35e-22 99.0
Msa1301650 AT4G08260.1 31.466 232 118 7 119 348 14 206 5.48e-22 93.6
Msa1301650 AT5G02760.1 28.975 283 145 10 123 354 71 348 1.38e-21 95.5
Msa1301650 AT3G16800.2 30.147 272 143 11 118 351 87 349 6.18e-20 90.5
Msa1301650 AT3G16800.1 30.147 272 143 11 118 351 87 349 6.18e-20 90.5
Msa1301650 AT3G16800.3 30.147 272 143 11 118 351 87 349 6.18e-20 90.5
Msa1301650 AT1G47380.1 28.819 288 153 12 121 384 61 320 4.38e-18 85.9
Msa1301650 AT3G27140.1 28.049 246 111 6 119 362 14 195 5.94e-18 82.8
Msa1301650 AT1G09160.2 30.147 272 140 11 121 363 65 315 1.03e-17 84.7
Msa1301650 AT1G09160.1 30.147 272 140 11 121 363 65 315 1.03e-17 84.7
Msa1301650 AT3G51370.1 26.645 304 144 13 123 360 78 368 2.16e-17 83.2
Msa1301650 AT3G51370.3 26.645 304 144 13 123 360 78 368 2.16e-17 83.2
Msa1301650 AT4G32950.1 26.207 290 171 10 93 356 50 322 2.37e-17 82.8
Msa1301650 AT3G16800.4 29.278 263 139 11 127 351 1 254 3.98e-17 80.9
Msa1301650 AT3G16800.6 29.278 263 139 11 127 351 1 254 3.98e-17 80.9
Msa1301650 AT1G22280.2 32.215 149 86 5 98 244 47 182 6.04e-17 79.0
Msa1301650 AT5G57050.3 32.450 151 86 4 124 260 160 308 9.58e-17 80.9
Msa1301650 AT4G38520.2 26.573 286 146 9 123 352 80 357 9.77e-17 81.6
Msa1301650 AT4G38520.1 26.573 286 146 9 123 352 80 357 9.77e-17 81.6
Msa1301650 AT3G12620.2 26.712 292 140 11 123 353 82 360 1.39e-16 80.9
Msa1301650 AT3G12620.1 26.712 292 140 11 123 353 82 360 1.39e-16 80.9
Msa1301650 AT3G05640.2 26.374 273 153 10 118 351 87 350 4.24e-16 79.3
Msa1301650 AT3G05640.1 26.374 273 153 10 118 351 87 350 4.24e-16 79.3
Msa1301650 AT3G05640.3 26.374 273 153 10 118 351 87 350 4.24e-16 79.3
Msa1301650 AT5G66080.1 27.668 253 138 11 123 337 81 326 1.11e-15 78.2
Msa1301650 AT3G55050.1 27.273 286 144 11 123 352 83 360 1.72e-15 77.8
Msa1301650 AT3G55050.2 27.273 286 144 11 123 352 83 360 1.72e-15 77.8
Msa1301650 AT5G59220.2 34.000 150 85 3 124 259 148 297 3.62e-15 75.9
Msa1301650 AT1G72770.2 30.488 164 85 3 124 258 238 401 1.13e-13 72.4
Msa1301650 AT1G72770.5 30.488 164 85 3 124 258 238 401 1.13e-13 72.4
Msa1301650 AT5G19280.1 27.757 263 152 11 109 352 336 579 3.94e-13 71.2
Msa1301650 AT3G16560.1 28.977 176 96 6 162 310 247 420 6.52e-13 70.5
Msa1301650 AT3G16560.4 28.977 176 96 6 162 310 247 420 6.52e-13 70.5
Msa1301650 AT3G16560.2 28.977 176 96 6 162 310 247 420 6.52e-13 70.5
Msa1301650 AT3G16560.3 28.977 176 96 6 162 310 247 420 7.02e-13 70.1
Msa1301650 AT4G32950.2 28.571 231 125 9 93 300 50 263 7.12e-13 68.9
Msa1301650 AT5G26010.1 24.290 317 175 11 81 356 38 330 1.14e-12 68.9
Msa1301650 AT5G01700.1 27.155 232 119 8 119 310 24 245 4.10e-12 67.0
Msa1301650 AT5G01700.2 27.155 232 119 8 119 310 73 294 8.78e-12 66.6
Msa1301650 AT5G27930.3 30.088 226 105 11 118 300 86 301 3.28e-11 64.3
Msa1301650 AT1G79630.6 29.714 175 96 5 156 312 83 248 6.28e-11 63.9
Msa1301650 AT1G79630.3 29.714 175 96 5 156 312 83 248 6.28e-11 63.9
Msa1301650 AT1G79630.4 29.714 175 96 5 156 312 83 248 6.28e-11 63.9
Msa1301650 AT1G79630.5 29.714 175 96 5 156 312 83 248 6.28e-11 63.9
Msa1301650 AT1G79630.2 29.714 175 96 5 156 312 123 288 7.76e-11 63.9
Msa1301650 AT1G79630.7 29.714 175 96 5 156 312 123 288 7.76e-11 63.9

Find 107 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTTGACTGCAATTATATT+TGG 0.173522 8_3:+63788872 Msa1301650:CDS
GAGGACTTGGCAAAGAAATT+TGG 0.243759 8_3:+63788451 Msa1301650:CDS
ATTGAGTCTTCCCTGTCTTC+TGG 0.285045 8_3:+63788842 Msa1301650:CDS
TAATTGGAAGTGATGGAATA+TGG 0.292557 8_3:+63789487 Msa1301650:CDS
TAGATGGCATACACCATAAA+AGG 0.303590 8_3:-63788503 None:intergenic
ATCCTTTCTTTCATGCATAA+AGG 0.303955 8_3:-63789282 None:intergenic
ATACTAACAGGAAATGAATT+AGG 0.315445 8_3:-63787544 None:intergenic
GTCAAACACATCGTTGTATA+TGG 0.317567 8_3:+63790025 Msa1301650:three_prime_UTR
TAGCAAAATCTACAGCGTTT+TGG 0.332140 8_3:-63789524 None:intergenic
GCATAAAGGCCTATGATCTT+TGG 0.333154 8_3:-63789268 None:intergenic
GCAAGTTCCTGAGCTGAAAA+AGG 0.336469 8_3:+63787318 Msa1301650:five_prime_UTR
TAGCCAATGCCGGGGACTGC+AGG 0.348722 8_3:+63789208 Msa1301650:CDS
TGATCAAGATAATAATAATA+TGG 0.349493 8_3:+63787405 Msa1301650:CDS
CAGGAACTTGCAGCTGTTGT+AGG 0.352613 8_3:-63787306 None:intergenic
GTGTAGGGACGAAATTTATC+AGG 0.353257 8_3:-63788363 None:intergenic
CAATGTTGCAAGGAAATAAT+AGG 0.358586 8_3:+63789579 Msa1301650:CDS
TTGATAACAGAAGGATTTCA+TGG 0.359698 8_3:+63787434 Msa1301650:CDS
TCTATTTATTAAACTTTACT+TGG 0.361012 8_3:+63790000 Msa1301650:three_prime_UTR
ATCAGAACCACCACCACCTT+TGG 0.374581 8_3:+63789659 Msa1301650:CDS
ATTGATGATGGTTACTTGAA+TGG 0.410247 8_3:+63789330 Msa1301650:CDS
ATTCTCAAAAGCATAGGAAT+AGG 0.413644 8_3:-63790086 None:intergenic
ATGCTTCTCCTGACTCTGTT+AGG 0.416065 8_3:-63789693 None:intergenic
GAATAATCTATCTTTCTAAC+AGG 0.434832 8_3:-63789746 None:intergenic
TTCTATTACCCCTCCAGCTC+GGG 0.442074 8_3:-63789241 None:intergenic
TATTCTGTGCAGGTATTTGA+TGG 0.443156 8_3:+63788674 Msa1301650:intron
TTCGTCCCTACACTTCGGTC+GGG 0.443590 8_3:+63788373 Msa1301650:CDS
ATGTGAGTATCCTCCATGTA+AGG 0.444464 8_3:-63788421 None:intergenic
TCACATATGCATTGAGGACT+TGG 0.445711 8_3:+63788438 Msa1301650:CDS
ATGAATTAGGAGCACTGATC+TGG 0.445790 8_3:-63787531 None:intergenic
CTTTACTTGTAGCCAATGCC+GGG 0.446990 8_3:+63789199 Msa1301650:CDS
AAGGAAGTGAGTGGAAGAAA+TGG 0.449462 8_3:+63789402 Msa1301650:CDS
TCGTCCCTACACTTCGGTCG+GGG 0.449528 8_3:+63788374 Msa1301650:CDS
CACCACCTTTGGTTGTTGAA+AGG 0.451444 8_3:+63789670 Msa1301650:CDS
AGTTCTGGCTCAGCACTTAG+TGG 0.454686 8_3:-63789426 None:intergenic
AGCCCTGCAGTCCCCGGCAT+TGG 0.455154 8_3:-63789211 None:intergenic
TCTTTACTTGTAGCCAATGC+CGG 0.457915 8_3:+63789198 Msa1301650:CDS
TGACGCGGGCTCTAGGCAAC+TGG 0.460183 8_3:+63789364 Msa1301650:CDS
CTAGGCGTGACGCGGGCTCT+AGG 0.463224 8_3:+63789357 Msa1301650:CDS
TTGAAAGGCCTAACAGAGTC+AGG 0.464128 8_3:+63789685 Msa1301650:CDS
AATGTCATCAATTTAAGTTC+TGG 0.464919 8_3:-63789441 None:intergenic
TATTGGAGGTCGCCCTTACA+TGG 0.466728 8_3:+63788408 Msa1301650:CDS
CTTGGCGGATTTATTGATGA+TGG 0.469287 8_3:+63789318 Msa1301650:CDS
TAGAATTGTCCAAAGATCAT+AGG 0.470481 8_3:+63789259 Msa1301650:CDS
AACAGTAACAAAGGAGTTGT+TGG 0.481782 8_3:-63787350 None:intergenic
CTAGGCAACTGGCATCTTGA+AGG 0.481839 8_3:+63789375 Msa1301650:CDS
GGCCTTTATGCATGAAAGAA+AGG 0.485159 8_3:+63789280 Msa1301650:CDS
CTATCTTTCCCACTAACAAT+GGG 0.504395 8_3:-63789930 None:intergenic
CAAAACAGCCCTGCAGTCCC+CGG 0.507129 8_3:-63789217 None:intergenic
TTTCGTCCCTACACTTCGGT+CGG 0.508489 8_3:+63788372 Msa1301650:CDS
AAGCAGTTGTACCAGAAGAC+AGG 0.513476 8_3:-63788853 None:intergenic
CTGGCATCTTGAAGGAATGA+AGG 0.515181 8_3:+63789383 Msa1301650:CDS
CTATTTATTAAACTTTACTT+GGG 0.518734 8_3:+63790001 Msa1301650:three_prime_UTR
TGCTAATCAGTGATCACTGG+TGG 0.521465 8_3:+63789839 Msa1301650:three_prime_UTR
TCAACAACCAAAGGTGGTGG+TGG 0.531756 8_3:-63789666 None:intergenic
AATTGGAAGTGATGGAATAT+GGG 0.533968 8_3:+63789488 Msa1301650:CDS
TGACTGCAATTATATTTGGA+AGG 0.542508 8_3:+63788876 Msa1301650:CDS
TCTATCTTTCCCACTAACAA+TGG 0.544525 8_3:-63789931 None:intergenic
AGGAGCACTGATCTGGTCAC+TGG 0.548106 8_3:-63787524 None:intergenic
AGCCAATGCCGGGGACTGCA+GGG 0.552576 8_3:+63789209 Msa1301650:CDS
TTGTGCTAATCAGTGATCAC+TGG 0.555066 8_3:+63789836 Msa1301650:three_prime_UTR
AGATAATTTAACTGTTGTGA+TGG 0.557874 8_3:+63789626 Msa1301650:CDS
TTGTTCGCGATCATCTGCCG+AGG 0.564331 8_3:+63788723 Msa1301650:CDS
ACTTGCAGCTGTTGTAGGCA+TGG 0.564631 8_3:-63787301 None:intergenic
TGGAGAAACTTGATAACAGA+AGG 0.570639 8_3:+63787425 Msa1301650:CDS
TATGCATGAAAGAAAGGATG+AGG 0.570968 8_3:+63789286 Msa1301650:CDS
ATTCTATTACCCCTCCAGCT+CGG 0.573089 8_3:-63789242 None:intergenic
CTTCCCTCTTGAACTCGAGA+AGG 0.574740 8_3:+63788766 Msa1301650:CDS
ATGAGGATCGAGTCTCTTGG+CGG 0.579884 8_3:+63789303 Msa1301650:CDS
CGTCCCTACACTTCGGTCGG+GGG 0.582208 8_3:+63788375 Msa1301650:CDS
GTGCAGGTATTTGATGGACA+TGG 0.586679 8_3:+63788680 Msa1301650:intron
TTAGGCCTTTCAACAACCAA+AGG 0.587308 8_3:-63789675 None:intergenic
ACCACCTTCTCGAGTTCAAG+AGG 0.589310 8_3:-63788770 None:intergenic
AGGATGAGGATCGAGTCTCT+TGG 0.591958 8_3:+63789300 Msa1301650:CDS
CATGTTGACAACAGTAACAA+AGG 0.593245 8_3:-63787359 None:intergenic
TGGACAGCTAGGCGTGACGC+GGG 0.593529 8_3:+63789350 Msa1301650:CDS
AACTCGAGAAGGTGGTCACA+AGG 0.594388 8_3:+63788777 Msa1301650:CDS
CTTTCAACAACCAAAGGTGG+TGG 0.598090 8_3:-63789669 None:intergenic
CAGGAGAAGCATATCTGCTG+AGG 0.600005 8_3:+63789704 Msa1301650:CDS
CCCTCTTGAACTCGAGAAGG+TGG 0.601383 8_3:+63788769 Msa1301650:CDS
AGCAGTTGTACCAGAAGACA+GGG 0.602803 8_3:-63788852 None:intergenic
ATGGACAGCTAGGCGTGACG+CGG 0.605836 8_3:+63789349 Msa1301650:CDS
GCATCCTCAACAATCACCCT+CGG 0.609381 8_3:-63788740 None:intergenic
GGTTACTTGAATGGACAGCT+AGG 0.611625 8_3:+63789339 Msa1301650:CDS
GGCCTTTCAACAACCAAAGG+TGG 0.612573 8_3:-63789672 None:intergenic
TAAATTTCGTCCCTACACTT+CGG 0.613927 8_3:+63788368 Msa1301650:CDS
GAAGGAATGAAGGAAGTGAG+TGG 0.614412 8_3:+63789393 Msa1301650:CDS
TTAAAAGAAAACAAACAGTA+CGG 0.614864 8_3:-63790051 None:intergenic
TTAGCTGGAAAGCATTTGTG+AGG 0.615709 8_3:+63788312 Msa1301650:intron
ACAAAGGAGTTGTTGGCAAG+TGG 0.616465 8_3:-63787343 None:intergenic
AGGAGAAGCATATCTGCTGA+GGG 0.620558 8_3:+63789705 Msa1301650:CDS
GCTAATCAGTGATCACTGGT+GGG 0.621957 8_3:+63789840 Msa1301650:three_prime_UTR
GGGGAGTGGTCTGATATTGG+AGG 0.624136 8_3:+63788394 Msa1301650:CDS
TGTTCGCGATCATCTGCCGA+GGG 0.626852 8_3:+63788724 Msa1301650:CDS
TGTGAGTATCCTCCATGTAA+GGG 0.630192 8_3:-63788420 None:intergenic
TGCAAAAGATCACAATGCAG+AGG 0.650781 8_3:-63787457 None:intergenic
GTCAACATGTGTGTGCAAGA+AGG 0.652909 8_3:+63787373 Msa1301650:exon
GGATACTCACATATGCATTG+AGG 0.657144 8_3:+63788432 Msa1301650:CDS
TGATGTGAAACAATGTTGCA+AGG 0.658172 8_3:+63789569 Msa1301650:CDS
CCACCTTCTCGAGTTCAAGA+GGG 0.662371 8_3:-63788769 None:intergenic
CTAATCAGTGATCACTGGTG+GGG 0.663545 8_3:+63789841 Msa1301650:three_prime_UTR
TTTACTTGTAGCCAATGCCG+GGG 0.668453 8_3:+63789200 Msa1301650:CDS
AGGTATTTGATGGACATGGA+GGG 0.676243 8_3:+63788684 Msa1301650:intron
TCTGCCGAGGGTGATTGTTG+AGG 0.686396 8_3:+63788736 Msa1301650:CDS
CAGGTATTTGATGGACATGG+AGG 0.705749 8_3:+63788683 Msa1301650:intron
TGGAGGTCGCCCTTACATGG+AGG 0.715266 8_3:+63788411 Msa1301650:CDS
ATAGGCGAAGCAATAAAGCG+AGG 0.718434 8_3:+63789597 Msa1301650:CDS
GTAGAGAAAATAATGCACTG+AGG 0.795756 8_3:+63789863 Msa1301650:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGAAATTATGATTTTTA+AGG + chr8_3:63788099-63788118 Msa1301650:intron 10.0%
!!! AATGAAATTATGATTTTTAA+GGG + chr8_3:63788100-63788119 Msa1301650:intron 10.0%
!! AAAGAATAATAATTTCTAAT+GGG + chr8_3:63789077-63789096 Msa1301650:intron 10.0%
!! AAAATAATGATAATTAAACA+AGG - chr8_3:63789168-63789187 None:intergenic 10.0%
!! TGATCAAGATAATAATAATA+TGG + chr8_3:63787405-63787424 Msa1301650:CDS 15.0%
!!! AGATAAGAATTTAAAACAAA+AGG - chr8_3:63788004-63788023 None:intergenic 15.0%
!!! CTTTTTTCTAAATTTGAAAT+TGG + chr8_3:63788030-63788049 Msa1301650:intron 15.0%
!! TAATCATGATTTAACATTAA+AGG + chr8_3:63788644-63788663 Msa1301650:intron 15.0%
!! GAAAGAATAATAATTTCTAA+TGG + chr8_3:63789076-63789095 Msa1301650:intron 15.0%
!! TCTATTTATTAAACTTTACT+TGG + chr8_3:63790000-63790019 Msa1301650:three_prime_UTR 15.0%
!! CTATTTATTAAACTTTACTT+GGG + chr8_3:63790001-63790020 Msa1301650:three_prime_UTR 15.0%
!! TAGCTTATAATATAACATAG+GGG - chr8_3:63787636-63787655 None:intergenic 20.0%
!! CTAGCTTATAATATAACATA+GGG - chr8_3:63787637-63787656 None:intergenic 20.0%
!!! ACTAGCTTATAATATAACAT+AGG - chr8_3:63787638-63787657 None:intergenic 20.0%
!!! GTTATATTATAAGCTAGTTT+TGG + chr8_3:63787640-63787659 Msa1301650:intron 20.0%
!!! TAGTTTTGGATTTTTTAAGA+TGG + chr8_3:63787654-63787673 Msa1301650:intron 20.0%
!! AATGCATAATTAAGCAATTA+AGG - chr8_3:63787960-63787979 None:intergenic 20.0%
!! AATTAACAAAATCAGTTAGA+TGG - chr8_3:63788522-63788541 None:intergenic 20.0%
!! AAAAGAAAATTTATGACAAC+AGG - chr8_3:63788546-63788565 None:intergenic 20.0%
!!! CATTTTTGTGATTATAAGAT+AGG + chr8_3:63788587-63788606 Msa1301650:intron 20.0%
!! TTAAAAGAAAACAAACAGTA+CGG - chr8_3:63790054-63790073 None:intergenic 20.0%
! ATACTAACAGGAAATGAATT+AGG - chr8_3:63787547-63787566 None:intergenic 25.0%
! GAAGAAAAGAAGAACAAAAA+TGG - chr8_3:63787614-63787633 None:intergenic 25.0%
!!! TTTAAGATGGTTTTCCAATA+AGG + chr8_3:63787667-63787686 Msa1301650:intron 25.0%
! AAGTATAATGAAAGTCTAAG+AGG - chr8_3:63787693-63787712 None:intergenic 25.0%
! TCTTAGACTTTCATTATACT+TGG + chr8_3:63787692-63787711 Msa1301650:intron 25.0%
! CTTAGACTTTCATTATACTT+GGG + chr8_3:63787693-63787712 Msa1301650:intron 25.0%
! TTAAGTTATTTATCCAAACC+AGG + chr8_3:63787934-63787953 Msa1301650:intron 25.0%
! ACATATCTAAGAGAATTTCA+AGG - chr8_3:63788134-63788153 None:intergenic 25.0%
! CTAATCACATCTTATATCAA+AGG - chr8_3:63788183-63788202 None:intergenic 25.0%
! GAAAATTGCATTCATAACAA+AGG + chr8_3:63788609-63788628 Msa1301650:intron 25.0%
! GATTTAACATTAAAGGCAAA+TGG + chr8_3:63788651-63788670 Msa1301650:intron 25.0%
!!! ATGGTCTTATGGTTATAAAA+TGG - chr8_3:63788928-63788947 None:intergenic 25.0%
!! GATAAAAATATTGACTGCTA+CGG + chr8_3:63789024-63789043 Msa1301650:intron 25.0%
!! ATAAAAATATTGACTGCTAC+GGG + chr8_3:63789025-63789044 Msa1301650:intron 25.0%
!! TTATCATTATTTTCCTGTAC+AGG + chr8_3:63789175-63789194 Msa1301650:intron 25.0%
!! AATGTCATCAATTTAAGTTC+TGG - chr8_3:63789444-63789463 None:intergenic 25.0%
!!! TTTTTGATAATTGGAAGTGA+TGG + chr8_3:63789480-63789499 Msa1301650:CDS 25.0%
! AGATAATTTAACTGTTGTGA+TGG + chr8_3:63789626-63789645 Msa1301650:CDS 25.0%
! GAATAATCTATCTTTCTAAC+AGG - chr8_3:63789749-63789768 None:intergenic 25.0%
! TTGATAACAGAAGGATTTCA+TGG + chr8_3:63787434-63787453 Msa1301650:CDS 30.0%
GAAGGAAATCAAATACTAAC+AGG - chr8_3:63787559-63787578 None:intergenic 30.0%
ACAAAAGAAAGAGAAGATGA+AGG - chr8_3:63787577-63787596 None:intergenic 30.0%
ACAAAAATGGCATAACAAGA+AGG - chr8_3:63787601-63787620 None:intergenic 30.0%
! GATTGACTTATTTGAACCTA+TGG + chr8_3:63787725-63787744 Msa1301650:intron 30.0%
!! TTCATAAGTGTTTTTGCCAT+AGG - chr8_3:63787744-63787763 None:intergenic 30.0%
ATCAGCTGAAAACAACTTAT+AGG - chr8_3:63787804-63787823 None:intergenic 30.0%
ATAATTAAGCAATTAAGGCC+TGG - chr8_3:63787955-63787974 None:intergenic 30.0%
!! ATAAGATGTGATTAGTCTCT+AGG + chr8_3:63788188-63788207 Msa1301650:intron 30.0%
!! TAAGATGTGATTAGTCTCTA+GGG + chr8_3:63788189-63788208 Msa1301650:intron 30.0%
!!! TTTCTGTGCATTATTTTAGC+TGG + chr8_3:63788297-63788316 Msa1301650:intron 30.0%
GCTTTGACTGCAATTATATT+TGG + chr8_3:63788872-63788891 Msa1301650:CDS 30.0%
TGACTGCAATTATATTTGGA+AGG + chr8_3:63788876-63788895 Msa1301650:CDS 30.0%
TATAAAATGGCAAAGAATGG+TGG - chr8_3:63788915-63788934 None:intergenic 30.0%
GGTTATAAAATGGCAAAGAA+TGG - chr8_3:63788918-63788937 None:intergenic 30.0%
! ATAACCATAAGACCATTTTG+TGG + chr8_3:63788932-63788951 Msa1301650:intron 30.0%
! ATTTTTATCTGTGTCGTGTT+CGG - chr8_3:63789013-63789032 None:intergenic 30.0%
TAGAATTGTCCAAAGATCAT+AGG + chr8_3:63789259-63789278 Msa1301650:CDS 30.0%
ATCCTTTCTTTCATGCATAA+AGG - chr8_3:63789285-63789304 None:intergenic 30.0%
!! ATTGATGATGGTTACTTGAA+TGG + chr8_3:63789330-63789349 Msa1301650:CDS 30.0%
! TAATTGGAAGTGATGGAATA+TGG + chr8_3:63789487-63789506 Msa1301650:CDS 30.0%
! AATTGGAAGTGATGGAATAT+GGG + chr8_3:63789488-63789507 Msa1301650:CDS 30.0%
CAATGTTGCAAGGAAATAAT+AGG + chr8_3:63789579-63789598 Msa1301650:CDS 30.0%
!! ATTGTTTTACCCATTGTTAG+TGG + chr8_3:63789921-63789940 Msa1301650:three_prime_UTR 30.0%
!! TTGTTTTACCCATTGTTAGT+GGG + chr8_3:63789922-63789941 Msa1301650:three_prime_UTR 30.0%
AACAGTAACAAAGGAGTTGT+TGG - chr8_3:63787353-63787372 None:intergenic 35.0%
CATGTTGACAACAGTAACAA+AGG - chr8_3:63787362-63787381 None:intergenic 35.0%
! TGGAGAAACTTGATAACAGA+AGG + chr8_3:63787425-63787444 Msa1301650:CDS 35.0%
! TGATCTTTTGCATGCTCAAA+TGG + chr8_3:63787468-63787487 Msa1301650:CDS 35.0%
! AGCTTGTCAAGATAGCTTTA+TGG + chr8_3:63787831-63787850 Msa1301650:intron 35.0%
TAAATTTCGTCCCTACACTT+CGG + chr8_3:63788368-63788387 Msa1301650:CDS 35.0%
!! GACGAAGCTATTTCCTTTTA+TGG + chr8_3:63788490-63788509 Msa1301650:CDS 35.0%
TAGATGGCATACACCATAAA+AGG - chr8_3:63788506-63788525 None:intergenic 35.0%
TATTCTGTGCAGGTATTTGA+TGG + chr8_3:63788674-63788693 Msa1301650:intron 35.0%
CAACACGACTATAACTCTAA+TGG - chr8_3:63789054-63789073 None:intergenic 35.0%
CATTAGAGTTATAGTCGTGT+TGG + chr8_3:63789052-63789071 Msa1301650:intron 35.0%
TATGATTGTATGCATTCCCT+AGG - chr8_3:63789142-63789161 None:intergenic 35.0%
TATGCATGAAAGAAAGGATG+AGG + chr8_3:63789286-63789305 Msa1301650:CDS 35.0%
!! GACGACGAGTTTTTGATAAT+TGG + chr8_3:63789471-63789490 Msa1301650:CDS 35.0%
! TAGCAAAATCTACAGCGTTT+TGG - chr8_3:63789527-63789546 None:intergenic 35.0%
TGATGTGAAACAATGTTGCA+AGG + chr8_3:63789569-63789588 Msa1301650:CDS 35.0%
!!! TCTCTGCAATGCCTTATTTT+TGG + chr8_3:63789770-63789789 Msa1301650:three_prime_UTR 35.0%
ATCTCAGCTCACCAAAAATA+AGG - chr8_3:63789784-63789803 None:intergenic 35.0%
GTAGAGAAAATAATGCACTG+AGG + chr8_3:63789863-63789882 Msa1301650:three_prime_UTR 35.0%
CTATCTTTCCCACTAACAAT+GGG - chr8_3:63789933-63789952 None:intergenic 35.0%
TCTATCTTTCCCACTAACAA+TGG - chr8_3:63789934-63789953 None:intergenic 35.0%
GTCAAACACATCGTTGTATA+TGG + chr8_3:63790025-63790044 Msa1301650:three_prime_UTR 35.0%
TGCAAAAGATCACAATGCAG+AGG - chr8_3:63787460-63787479 None:intergenic 40.0%
!! ATGAATTAGGAGCACTGATC+TGG - chr8_3:63787534-63787553 None:intergenic 40.0%
! GAAAGTCTAAGAGGCCTTAT+TGG - chr8_3:63787684-63787703 None:intergenic 40.0%
TAAGCAATTAAGGCCTGGTT+TGG - chr8_3:63787950-63787969 None:intergenic 40.0%
!! TTAGCTGGAAAGCATTTGTG+AGG + chr8_3:63788312-63788331 Msa1301650:intron 40.0%
GTGTAGGGACGAAATTTATC+AGG - chr8_3:63788366-63788385 None:intergenic 40.0%
TGTGAGTATCCTCCATGTAA+GGG - chr8_3:63788423-63788442 None:intergenic 40.0%
ATGTGAGTATCCTCCATGTA+AGG - chr8_3:63788424-63788443 None:intergenic 40.0%
GGATACTCACATATGCATTG+AGG + chr8_3:63788432-63788451 Msa1301650:CDS 40.0%
TCACATATGCATTGAGGACT+TGG + chr8_3:63788438-63788457 Msa1301650:CDS 40.0%
GAGGACTTGGCAAAGAAATT+TGG + chr8_3:63788451-63788470 Msa1301650:CDS 40.0%
! AGGTATTTGATGGACATGGA+GGG + chr8_3:63788684-63788703 Msa1301650:intron 40.0%
! CTCTCCACAAAATGGTCTTA+TGG - chr8_3:63788939-63788958 None:intergenic 40.0%
!! ACACATGTCGCTATTTTCCT+AGG + chr8_3:63789122-63789141 Msa1301650:intron 40.0%
!! CACATGTCGCTATTTTCCTA+GGG + chr8_3:63789123-63789142 Msa1301650:intron 40.0%
CTACAAGTAAAGACCTGTAC+AGG - chr8_3:63789191-63789210 None:intergenic 40.0%
TCTTTACTTGTAGCCAATGC+CGG + chr8_3:63789198-63789217 Msa1301650:CDS 40.0%
GCATAAAGGCCTATGATCTT+TGG - chr8_3:63789271-63789290 None:intergenic 40.0%
GGCCTTTATGCATGAAAGAA+AGG + chr8_3:63789280-63789299 Msa1301650:CDS 40.0%
!! CTTGGCGGATTTATTGATGA+TGG + chr8_3:63789318-63789337 Msa1301650:CDS 40.0%
AAGGAAGTGAGTGGAAGAAA+TGG + chr8_3:63789402-63789421 Msa1301650:CDS 40.0%
! ACGCTGTAGATTTTGCTAGA+AGG + chr8_3:63789529-63789548 Msa1301650:CDS 40.0%
! CTGTAGATTTTGCTAGAAGG+AGG + chr8_3:63789532-63789551 Msa1301650:CDS 40.0%
TTAGGCCTTTCAACAACCAA+AGG - chr8_3:63789678-63789697 None:intergenic 40.0%
TTGTGCTAATCAGTGATCAC+TGG + chr8_3:63789836-63789855 Msa1301650:three_prime_UTR 40.0%
GCAAGTTCCTGAGCTGAAAA+AGG + chr8_3:63787318-63787337 Msa1301650:five_prime_UTR 45.0%
!!! AGTGGTACCTTTTTCAGCTC+AGG - chr8_3:63787328-63787347 None:intergenic 45.0%
ACAAAGGAGTTGTTGGCAAG+TGG - chr8_3:63787346-63787365 None:intergenic 45.0%
GTCAACATGTGTGTGCAAGA+AGG + chr8_3:63787373-63787392 Msa1301650:exon 45.0%
AGGCAAATGGTATTCTGTGC+AGG + chr8_3:63788664-63788683 Msa1301650:intron 45.0%
! GTGCAGGTATTTGATGGACA+TGG + chr8_3:63788680-63788699 Msa1301650:intron 45.0%
! CAGGTATTTGATGGACATGG+AGG + chr8_3:63788683-63788702 Msa1301650:intron 45.0%
!! GGTGGTCACAAGGTCATTTT+TGG + chr8_3:63788787-63788806 Msa1301650:CDS 45.0%
ATTGAGTCTTCCCTGTCTTC+TGG + chr8_3:63788842-63788861 Msa1301650:CDS 45.0%
AGCAGTTGTACCAGAAGACA+GGG - chr8_3:63788855-63788874 None:intergenic 45.0%
AAGCAGTTGTACCAGAAGAC+AGG - chr8_3:63788856-63788875 None:intergenic 45.0%
TTGTGCGACTCTCCACAAAA+TGG - chr8_3:63788947-63788966 None:intergenic 45.0%
GTGTCAGACATCTCGTTTAG+AGG - chr8_3:63789106-63789125 None:intergenic 45.0%
CTTTACTTGTAGCCAATGCC+GGG + chr8_3:63789199-63789218 Msa1301650:CDS 45.0%
TTTACTTGTAGCCAATGCCG+GGG + chr8_3:63789200-63789219 Msa1301650:CDS 45.0%
ATTCTATTACCCCTCCAGCT+CGG - chr8_3:63789245-63789264 None:intergenic 45.0%
GGTTACTTGAATGGACAGCT+AGG + chr8_3:63789339-63789358 Msa1301650:CDS 45.0%
CTGGCATCTTGAAGGAATGA+AGG + chr8_3:63789383-63789402 Msa1301650:CDS 45.0%
GAAGGAATGAAGGAAGTGAG+TGG + chr8_3:63789393-63789412 Msa1301650:CDS 45.0%
ATAGGCGAAGCAATAAAGCG+AGG + chr8_3:63789597-63789616 Msa1301650:CDS 45.0%
CTTTCAACAACCAAAGGTGG+TGG - chr8_3:63789672-63789691 None:intergenic 45.0%
CACCACCTTTGGTTGTTGAA+AGG + chr8_3:63789670-63789689 Msa1301650:CDS 45.0%
TTGAAAGGCCTAACAGAGTC+AGG + chr8_3:63789685-63789704 Msa1301650:CDS 45.0%
ATGCTTCTCCTGACTCTGTT+AGG - chr8_3:63789696-63789715 None:intergenic 45.0%
! AGGAGAAGCATATCTGCTGA+GGG + chr8_3:63789705-63789724 Msa1301650:CDS 45.0%
TGCTAATCAGTGATCACTGG+TGG + chr8_3:63789839-63789858 Msa1301650:three_prime_UTR 45.0%
GCTAATCAGTGATCACTGGT+GGG + chr8_3:63789840-63789859 Msa1301650:three_prime_UTR 45.0%
CTAATCAGTGATCACTGGTG+GGG + chr8_3:63789841-63789860 Msa1301650:three_prime_UTR 45.0%
!!! TATACATTTTATTTTAAATT+TGG - chr8_3:63789891-63789910 None:intergenic 5.0%
ACTTGCAGCTGTTGTAGGCA+TGG - chr8_3:63787304-63787323 None:intergenic 50.0%
CAGGAACTTGCAGCTGTTGT+AGG - chr8_3:63787309-63787328 None:intergenic 50.0%
TTTCGTCCCTACACTTCGGT+CGG + chr8_3:63788372-63788391 Msa1301650:CDS 50.0%
TATTGGAGGTCGCCCTTACA+TGG + chr8_3:63788408-63788427 Msa1301650:CDS 50.0%
GCATCCTCAACAATCACCCT+CGG - chr8_3:63788743-63788762 None:intergenic 50.0%
CTTCCCTCTTGAACTCGAGA+AGG + chr8_3:63788766-63788785 Msa1301650:CDS 50.0%
CCACCTTCTCGAGTTCAAGA+GGG - chr8_3:63788772-63788791 None:intergenic 50.0%
ACCACCTTCTCGAGTTCAAG+AGG - chr8_3:63788773-63788792 None:intergenic 50.0%
AACTCGAGAAGGTGGTCACA+AGG + chr8_3:63788777-63788796 Msa1301650:CDS 50.0%
TGTGGAGAGTCGCACAACTT+TGG + chr8_3:63788950-63788969 Msa1301650:intron 50.0%
GTGGAGAGTCGCACAACTTT+GGG + chr8_3:63788951-63788970 Msa1301650:intron 50.0%
!! TGTTCGGTGTCTGTGCATCT+TGG - chr8_3:63788997-63789016 None:intergenic 50.0%
TTCTATTACCCCTCCAGCTC+GGG - chr8_3:63789244-63789263 None:intergenic 50.0%
AGGATGAGGATCGAGTCTCT+TGG + chr8_3:63789300-63789319 Msa1301650:CDS 50.0%
ATGAGGATCGAGTCTCTTGG+CGG + chr8_3:63789303-63789322 Msa1301650:CDS 50.0%
CTAGGCAACTGGCATCTTGA+AGG + chr8_3:63789375-63789394 Msa1301650:CDS 50.0%
!! AGTTCTGGCTCAGCACTTAG+TGG - chr8_3:63789429-63789448 None:intergenic 50.0%
ATCAGAACCACCACCACCTT+TGG + chr8_3:63789659-63789678 Msa1301650:CDS 50.0%
! TCAACAACCAAAGGTGGTGG+TGG - chr8_3:63789669-63789688 None:intergenic 50.0%
GGCCTTTCAACAACCAAAGG+TGG - chr8_3:63789675-63789694 None:intergenic 50.0%
! CAGGAGAAGCATATCTGCTG+AGG + chr8_3:63789704-63789723 Msa1301650:CDS 50.0%
!! AGGAGCACTGATCTGGTCAC+TGG - chr8_3:63787527-63787546 None:intergenic 55.0%
TTCGTCCCTACACTTCGGTC+GGG + chr8_3:63788373-63788392 Msa1301650:CDS 55.0%
TCGGGGGAGTGGTCTGATAT+TGG + chr8_3:63788391-63788410 Msa1301650:CDS 55.0%
GGGGAGTGGTCTGATATTGG+AGG + chr8_3:63788394-63788413 Msa1301650:CDS 55.0%
TTGTTCGCGATCATCTGCCG+AGG + chr8_3:63788723-63788742 Msa1301650:CDS 55.0%
TGTTCGCGATCATCTGCCGA+GGG + chr8_3:63788724-63788743 Msa1301650:CDS 55.0%
TCTGCCGAGGGTGATTGTTG+AGG + chr8_3:63788736-63788755 Msa1301650:CDS 55.0%
CCCTCTTGAACTCGAGAAGG+TGG + chr8_3:63788769-63788788 Msa1301650:CDS 55.0%
GAGTCGCACAACTTTGGGTC+AGG + chr8_3:63788956-63788975 Msa1301650:intron 55.0%
GGTGTCTGTGCATCTTGGCT+AGG - chr8_3:63788992-63789011 None:intergenic 55.0%
! CTGTTTTGTCCCGAGCTGGA+GGG + chr8_3:63789232-63789251 Msa1301650:CDS 55.0%
! TGTTTTGTCCCGAGCTGGAG+GGG + chr8_3:63789233-63789252 Msa1301650:CDS 55.0%
TCGTCCCTACACTTCGGTCG+GGG + chr8_3:63788374-63788393 Msa1301650:CDS 60.0%
ACTCCCCCGACCGAAGTGTA+GGG - chr8_3:63788381-63788400 None:intergenic 60.0%
TGGAGGTCGCCCTTACATGG+AGG + chr8_3:63788411-63788430 Msa1301650:CDS 60.0%
CAAAACAGCCCTGCAGTCCC+CGG - chr8_3:63789220-63789239 None:intergenic 60.0%
!! AGGGCTGTTTTGTCCCGAGC+TGG + chr8_3:63789228-63789247 Msa1301650:CDS 60.0%
!! GCTGTTTTGTCCCGAGCTGG+AGG + chr8_3:63789231-63789250 Msa1301650:CDS 60.0%
ATGGACAGCTAGGCGTGACG+CGG + chr8_3:63789349-63789368 Msa1301650:CDS 60.0%
CGTCCCTACACTTCGGTCGG+GGG + chr8_3:63788375-63788394 Msa1301650:CDS 65.0%
CACTCCCCCGACCGAAGTGT+AGG - chr8_3:63788382-63788401 None:intergenic 65.0%
CTACACTTCGGTCGGGGGAG+TGG + chr8_3:63788380-63788399 Msa1301650:CDS 65.0%
TAGCCAATGCCGGGGACTGC+AGG + chr8_3:63789208-63789227 Msa1301650:CDS 65.0%
AGCCAATGCCGGGGACTGCA+GGG + chr8_3:63789209-63789228 Msa1301650:CDS 65.0%
TGGACAGCTAGGCGTGACGC+GGG + chr8_3:63789350-63789369 Msa1301650:CDS 65.0%
!! TGACGCGGGCTCTAGGCAAC+TGG + chr8_3:63789364-63789383 Msa1301650:CDS 65.0%
AGCCCTGCAGTCCCCGGCAT+TGG - chr8_3:63789214-63789233 None:intergenic 70.0%
CTAGGCGTGACGCGGGCTCT+AGG + chr8_3:63789357-63789376 Msa1301650:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 63787162 63790095 63787162 ID=Msa1301650;Name=Msa1301650
chr8_3 mRNA 63787162 63790095 63787162 ID=Msa1301650-mRNA-1;Parent=Msa1301650;Name=Msa1301650-mRNA-1;_AED=0.05;_eAED=0.05;_QI=217|1|1|1|1|1|4|334|384
chr8_3 exon 63787162 63787558 63787162 ID=Msa1301650-mRNA-1:exon:19129;Parent=Msa1301650-mRNA-1
chr8_3 exon 63788316 63788513 63788316 ID=Msa1301650-mRNA-1:exon:19130;Parent=Msa1301650-mRNA-1
chr8_3 exon 63788686 63788897 63788686 ID=Msa1301650-mRNA-1:exon:19131;Parent=Msa1301650-mRNA-1
chr8_3 exon 63789197 63790095 63789197 ID=Msa1301650-mRNA-1:exon:19132;Parent=Msa1301650-mRNA-1
chr8_3 five_prime_UTR 63787162 63787378 63787162 ID=Msa1301650-mRNA-1:five_prime_utr;Parent=Msa1301650-mRNA-1
chr8_3 CDS 63787379 63787558 63787379 ID=Msa1301650-mRNA-1:cds;Parent=Msa1301650-mRNA-1
chr8_3 CDS 63788316 63788513 63788316 ID=Msa1301650-mRNA-1:cds;Parent=Msa1301650-mRNA-1
chr8_3 CDS 63788686 63788897 63788686 ID=Msa1301650-mRNA-1:cds;Parent=Msa1301650-mRNA-1
chr8_3 CDS 63789197 63789761 63789197 ID=Msa1301650-mRNA-1:cds;Parent=Msa1301650-mRNA-1
chr8_3 three_prime_UTR 63789762 63790095 63789762 ID=Msa1301650-mRNA-1:three_prime_utr;Parent=Msa1301650-mRNA-1
Gene Sequence

>Msa1301650

ATGTGTGTGCAAGAAGGTGAACAAGTTGATCAAGATAATAATAATATGGAGAAACTTGATAACAGAAGGATTTCATGGCCTCTGCATTGTGATCTTTTGCATGCTCAAATGGATAATTCAGAAAGAGACTCTTCTATTAGAATTTCCAGTGACCAGATCAGTGCTCCTAATTCATTTCCTCTGGAAAGCATTTGTGAGGATGCAGTGATTGCTGATAAAAAACAGAACCTGATAAATTTCGTCCCTACACTTCGGTCGGGGGAGTGGTCTGATATTGGAGGTCGCCCTTACATGGAGGATACTCACATATGCATTGAGGACTTGGCAAAGAAATTTGGTTACGATGTTGTCTGCGACGAAGCTATTTCCTTTTATGGTGTATTTGATGGACATGGAGGGAAGAGTGCTGCGCAATTTGTTCGCGATCATCTGCCGAGGGTGATTGTTGAGGATGCTGACTTCCCTCTTGAACTCGAGAAGGTGGTCACAAGGTCATTTTTGGAGACTGATTCAGAGTTTGCAAAAACATGCTCTATTGAGTCTTCCCTGTCTTCTGGTACAACTGCTTTGACTGCAATTATATTTGGAAGGTCTTTACTTGTAGCCAATGCCGGGGACTGCAGGGCTGTTTTGTCCCGAGCTGGAGGGGTAATAGAATTGTCCAAAGATCATAGGCCTTTATGCATGAAAGAAAGGATGAGGATCGAGTCTCTTGGCGGATTTATTGATGATGGTTACTTGAATGGACAGCTAGGCGTGACGCGGGCTCTAGGCAACTGGCATCTTGAAGGAATGAAGGAAGTGAGTGGAAGAAATGGACCACTAAGTGCTGAGCCAGAACTTAAATTGATGACATTGACAAAAGACGACGAGTTTTTGATAATTGGAAGTGATGGAATATGGGATGTTTTTCGTAGCCAAAACGCTGTAGATTTTGCTAGAAGGAGGCTTCAAGAGCACAATGATGTGAAACAATGTTGCAAGGAAATAATAGGCGAAGCAATAAAGCGAGGAGCAACAGATAATTTAACTGTTGTGATGGTATGTTTTCAATCAGAACCACCACCACCTTTGGTTGTTGAAAGGCCTAACAGAGTCAGGAGAAGCATATCTGCTGAGGGACTTCAGAATCTTAAATACCTGTTAGAAAGATAG
Protein sequence

>Msa1301650

MCVQEGEQVDQDNNNMEKLDNRRISWPLHCDLLHAQMDNSERDSSIRISSDQISAPNSFPLESICEDAVIADKKQNLINFVPTLRSGEWSDIGGRPYMEDTHICIEDLAKKFGYDVVCDEAISFYGVFDGHGGKSAAQFVRDHLPRVIVEDADFPLELEKVVTRSFLETDSEFAKTCSIESSLSSGTTALTAIIFGRSLLVANAGDCRAVLSRAGGVIELSKDHRPLCMKERMRIESLGGFIDDGYLNGQLGVTRALGNWHLEGMKEVSGRNGPLSAEPELKLMTLTKDDEFLIIGSDGIWDVFRSQNAVDFARRRLQEHNDVKQCCKEIIGEAIKRGATDNLTVVMVCFQSEPPPPLVVERPNRVRRSISAEGLQNLKYLLER*