Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1215910 | G7JHU0 | 87.156 | 109 | 14 | 0 | 1 | 109 | 1 | 109 | 2.44e-58 | 184 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0005350 | Msa1215910 | 0.809325 | 2.000628e-50 | -8.615850e-47 |
| Msa0053900 | Msa1215910 | 0.814161 | 1.774101e-51 | -8.615850e-47 |
| Msa0120340 | Msa1215910 | 0.800942 | 1.137235e-48 | -8.615850e-47 |
| Msa0697400 | Msa1215910 | 0.801148 | 1.032351e-48 | -8.615850e-47 |
| Msa0748720 | Msa1215910 | 0.995073 | 9.010444e-213 | -8.615850e-47 |
| Msa0148360 | Msa1215910 | 0.809276 | 2.049674e-50 | -8.615850e-47 |
| Msa0426330 | Msa1215910 | 0.808731 | 2.681337e-50 | -8.615850e-47 |
| Msa0993530 | Msa1215910 | 0.805076 | 1.589864e-49 | -8.615850e-47 |
| Msa0328210 | Msa1215910 | 0.802426 | 5.643257e-49 | -8.615850e-47 |
| Msa1175330 | Msa1215910 | 0.800045 | 1.732461e-48 | -8.615850e-47 |
| Msa1215910 | Msa1235630 | 0.817562 | 3.091958e-52 | -8.615850e-47 |
| Msa1215910 | Msa1315870 | 0.816332 | 5.841547e-52 | -8.615850e-47 |
| Msa0899230 | Msa1215910 | 0.801739 | 7.810342e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1215910 | MtrunA17_Chr4g0065281 | 87.156 | 109 | 14 | 0 | 1 | 109 | 4 | 112 | 5.64e-62 | 184 |
| Msa1215910 | MtrunA17_Chr2g0305061 | 40.741 | 108 | 54 | 4 | 1 | 107 | 1 | 99 | 1.75e-19 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1215910 | AT2G46490.1 | 45.588 | 68 | 30 | 2 | 4 | 64 | 2 | 69 | 2.24e-15 | 67.4 |
Find 25 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTACCAAACTGATCATTAC+TGG | 0.303232 | 8_1:+77371769 | None:intergenic |
| TGTTACCAAGTTGTTGTAAC+AGG | 0.325550 | 8_1:-77371894 | Msa1215910:CDS |
| TCTCCTCCTCCTGCTCTTGA+AGG | 0.330451 | 8_1:+77371746 | None:intergenic |
| CTCCTACGTTGTTTCTTGAT+AGG | 0.341022 | 8_1:+77371859 | None:intergenic |
| CAAGAAACAACGTAGGAGTT+TGG | 0.397063 | 8_1:-77371854 | Msa1215910:CDS |
| CTGCTGGAGATGGATTGTTC+TGG | 0.413714 | 8_1:-77372032 | Msa1215910:CDS |
| TTCTCCAGTAATGATCAGTT+TGG | 0.417813 | 8_1:-77371773 | Msa1215910:CDS |
| TTGTATGAAGGAAGCATCTC+TGG | 0.417867 | 8_1:-77372006 | Msa1215910:CDS |
| GAAGTACACAACCACATTTA+CGG | 0.417895 | 8_1:+77371950 | None:intergenic |
| ATATATGATATGGCCACTGC+TGG | 0.465999 | 8_1:-77372048 | None:intergenic |
| TTTGGTAAACCTTCAAGAGC+AGG | 0.522818 | 8_1:-77371755 | Msa1215910:CDS |
| AGGAGGAGGAGATTGATTCA+AGG | 0.527577 | 8_1:-77371735 | Msa1215910:CDS |
| CAACCACATTTACGGTGGTA+AGG | 0.548420 | 8_1:+77371958 | None:intergenic |
| GATATGGCCACTGCTGGAGA+TGG | 0.558779 | 8_1:-77372042 | Msa1215910:CDS |
| ACAATCCTGTTACAACAACT+TGG | 0.566427 | 8_1:+77371889 | None:intergenic |
| GGAGATTGATTCAAGGTCTG+AGG | 0.567181 | 8_1:-77371728 | Msa1215910:CDS |
| TTGTTCTGGTCATTGTATGA+AGG | 0.575432 | 8_1:-77372018 | Msa1215910:CDS |
| AAACCTTCAAGAGCAGGAGG+AGG | 0.580819 | 8_1:-77371749 | Msa1215910:CDS |
| ACTGGAGAAGAAGAATCAGT+TGG | 0.591197 | 8_1:+77371787 | None:intergenic |
| AGACCTTACCACCGTAAATG+TGG | 0.625137 | 8_1:-77371961 | Msa1215910:CDS |
| GGTAAACCTTCAAGAGCAGG+AGG | 0.637542 | 8_1:-77371752 | Msa1215910:CDS |
| GAACAATCCATCTCCAGCAG+TGG | 0.647354 | 8_1:+77372035 | None:intergenic |
| TACAACAACTTGGTAACATG+TGG | 0.679176 | 8_1:+77371899 | None:intergenic |
| ATCCTATCAAGAAACAACGT+AGG | 0.723502 | 8_1:-77371861 | Msa1215910:CDS |
| GTACACAACCACATTTACGG+TGG | 0.747880 | 8_1:+77371953 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTTACCAAACTGATCATTAC+TGG | + | chr8_1:77371993-77372012 | None:intergenic | 30.0% | |
| TTGTTCTGGTCATTGTATGA+AGG | - | chr8_1:77371741-77371760 | Msa1215910:CDS | 35.0% | |
| GAAGTACACAACCACATTTA+CGG | + | chr8_1:77371812-77371831 | None:intergenic | 35.0% | |
| ! | TACAACAACTTGGTAACATG+TGG | + | chr8_1:77371863-77371882 | None:intergenic | 35.0% |
| TGTTACCAAGTTGTTGTAAC+AGG | - | chr8_1:77371865-77371884 | Msa1215910:CDS | 35.0% | |
| ACAATCCTGTTACAACAACT+TGG | + | chr8_1:77371873-77371892 | None:intergenic | 35.0% | |
| ATCCTATCAAGAAACAACGT+AGG | - | chr8_1:77371898-77371917 | Msa1215910:CDS | 35.0% | |
| TTCTCCAGTAATGATCAGTT+TGG | - | chr8_1:77371986-77372005 | Msa1215910:CDS | 35.0% | |
| !! | TTGTATGAAGGAAGCATCTC+TGG | - | chr8_1:77371753-77371772 | Msa1215910:CDS | 40.0% |
| !! | CTCCTACGTTGTTTCTTGAT+AGG | + | chr8_1:77371903-77371922 | None:intergenic | 40.0% |
| CAAGAAACAACGTAGGAGTT+TGG | - | chr8_1:77371905-77371924 | Msa1215910:CDS | 40.0% | |
| ACTGGAGAAGAAGAATCAGT+TGG | + | chr8_1:77371975-77371994 | None:intergenic | 40.0% | |
| TTTGGTAAACCTTCAAGAGC+AGG | - | chr8_1:77372004-77372023 | Msa1215910:CDS | 40.0% | |
| AGACCTTACCACCGTAAATG+TGG | - | chr8_1:77371798-77371817 | Msa1215910:CDS | 45.0% | |
| CAACCACATTTACGGTGGTA+AGG | + | chr8_1:77371804-77371823 | None:intergenic | 45.0% | |
| GTACACAACCACATTTACGG+TGG | + | chr8_1:77371809-77371828 | None:intergenic | 45.0% | |
| ! | AGGAGGAGGAGATTGATTCA+AGG | - | chr8_1:77372024-77372043 | Msa1215910:CDS | 45.0% |
| ! | GGAGATTGATTCAAGGTCTG+AGG | - | chr8_1:77372031-77372050 | Msa1215910:CDS | 45.0% |
| GAACAATCCATCTCCAGCAG+TGG | + | chr8_1:77371727-77371746 | None:intergenic | 50.0% | |
| !! | CTGCTGGAGATGGATTGTTC+TGG | - | chr8_1:77371727-77371746 | Msa1215910:CDS | 50.0% |
| GGTAAACCTTCAAGAGCAGG+AGG | - | chr8_1:77372007-77372026 | Msa1215910:CDS | 50.0% | |
| AAACCTTCAAGAGCAGGAGG+AGG | - | chr8_1:77372010-77372029 | Msa1215910:CDS | 50.0% | |
| TCTCCTCCTCCTGCTCTTGA+AGG | + | chr8_1:77372016-77372035 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 77371720 | 77372061 | 77371720 | ID=Msa1215910;Name=Msa1215910 |
| chr8_1 | mRNA | 77371720 | 77372061 | 77371720 | ID=Msa1215910-mRNA-1;Parent=Msa1215910;Name=Msa1215910-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|-1|0|1|-1|1|1|0|113 |
| chr8_1 | exon | 77371720 | 77372061 | 77371720 | ID=Msa1215910-mRNA-1:exon:26687;Parent=Msa1215910-mRNA-1 |
| chr8_1 | CDS | 77371720 | 77372061 | 77371720 | ID=Msa1215910-mRNA-1:cds;Parent=Msa1215910-mRNA-1 |
| Gene Sequence |
| Protein sequence |
