Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748720 | AES91719.1 | 87.156 | 109 | 14 | 0 | 1 | 109 | 1 | 109 | 5.10e-58 | 184 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748720 | G7JHU0 | 87.156 | 109 | 14 | 0 | 1 | 109 | 1 | 109 | 2.44e-58 | 184 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0005350 | Msa0748720 | 0.810124 | 1.347486e-50 | -8.615850e-47 |
| Msa0053900 | Msa0748720 | 0.817529 | 3.145937e-52 | -8.615850e-47 |
| Msa0099730 | Msa0748720 | 0.800741 | 1.250351e-48 | -8.615850e-47 |
| Msa0120340 | Msa0748720 | 0.805014 | 1.637902e-49 | -8.615850e-47 |
| Msa0697400 | Msa0748720 | 0.800891 | 1.164832e-48 | -8.615850e-47 |
| Msa0748720 | Msa0899230 | 0.803979 | 2.692148e-49 | -8.615850e-47 |
| Msa0748720 | Msa0993530 | 0.806830 | 6.798418e-50 | -8.615850e-47 |
| Msa0748720 | Msa1175330 | 0.800685 | 1.283736e-48 | -8.615850e-47 |
| Msa0748720 | Msa1215910 | 0.995073 | 9.010444e-213 | -8.615850e-47 |
| Msa0748720 | Msa1235630 | 0.816401 | 5.635665e-52 | -8.615850e-47 |
| Msa0748720 | Msa1315870 | 0.820208 | 7.745706e-53 | -8.615850e-47 |
| Msa0148360 | Msa0748720 | 0.812836 | 3.469732e-51 | -8.615850e-47 |
| Msa0426330 | Msa0748720 | 0.812088 | 5.055305e-51 | -8.615850e-47 |
| Msa0328210 | Msa0748720 | 0.802713 | 4.923791e-49 | -8.615850e-47 |
| Msa0333390 | Msa0748720 | 0.802675 | 5.013008e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748720 | MtrunA17_Chr4g0065281 | 87.156 | 109 | 14 | 0 | 1 | 109 | 4 | 112 | 5.64e-62 | 184 |
| Msa0748720 | MtrunA17_Chr2g0305061 | 40.741 | 108 | 54 | 4 | 1 | 107 | 1 | 99 | 1.75e-19 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748720 | AT2G46490.1 | 45.588 | 68 | 30 | 2 | 4 | 64 | 2 | 69 | 2.24e-15 | 67.4 |
Find 25 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTACCAAACTGATCATTAC+TGG | 0.303232 | 5_2:+1984719 | None:intergenic |
| TGTTACCAAGTTGTTGTAAC+AGG | 0.325550 | 5_2:-1984844 | Msa0748720:CDS |
| TCTCCTCCTCCTGCTCTTGA+AGG | 0.330451 | 5_2:+1984696 | None:intergenic |
| CTCCTACGTTGTTTCTTGAT+AGG | 0.341022 | 5_2:+1984809 | None:intergenic |
| CAAGAAACAACGTAGGAGTT+TGG | 0.397063 | 5_2:-1984804 | Msa0748720:CDS |
| CTGCTGGAGATGGATTGTTC+TGG | 0.413714 | 5_2:-1984982 | Msa0748720:CDS |
| TTCTCCAGTAATGATCAGTT+TGG | 0.417813 | 5_2:-1984723 | Msa0748720:CDS |
| TTGTATGAAGGAAGCATCTC+TGG | 0.417867 | 5_2:-1984956 | Msa0748720:CDS |
| GAAGTACACAACCACATTTA+CGG | 0.417895 | 5_2:+1984900 | None:intergenic |
| ATATATGATATGGCCACTGC+TGG | 0.465999 | 5_2:-1984998 | None:intergenic |
| TTTGGTAAACCTTCAAGAGC+AGG | 0.522818 | 5_2:-1984705 | Msa0748720:CDS |
| AGGAGGAGGAGATTGATTCA+AGG | 0.527577 | 5_2:-1984685 | Msa0748720:CDS |
| CAACCACATTTACGGTGGTA+AGG | 0.548420 | 5_2:+1984908 | None:intergenic |
| GATATGGCCACTGCTGGAGA+TGG | 0.558779 | 5_2:-1984992 | Msa0748720:CDS |
| ACAATCCTGTTACAACAACT+TGG | 0.566427 | 5_2:+1984839 | None:intergenic |
| GGAGATTGATTCAAGGTCTG+AGG | 0.567181 | 5_2:-1984678 | Msa0748720:CDS |
| TTGTTCTGGTCATTGTATGA+AGG | 0.575432 | 5_2:-1984968 | Msa0748720:CDS |
| AAACCTTCAAGAGCAGGAGG+AGG | 0.580819 | 5_2:-1984699 | Msa0748720:CDS |
| ACTGGAGAAGAAGAATCAGT+TGG | 0.591197 | 5_2:+1984737 | None:intergenic |
| AGACCTTACCACCGTAAATG+TGG | 0.625137 | 5_2:-1984911 | Msa0748720:CDS |
| GGTAAACCTTCAAGAGCAGG+AGG | 0.637542 | 5_2:-1984702 | Msa0748720:CDS |
| GAACAATCCATCTCCAGCAG+TGG | 0.647354 | 5_2:+1984985 | None:intergenic |
| TACAACAACTTGGTAACATG+TGG | 0.679176 | 5_2:+1984849 | None:intergenic |
| ATCCTATCAAGAAACAACGT+AGG | 0.723502 | 5_2:-1984811 | Msa0748720:CDS |
| GTACACAACCACATTTACGG+TGG | 0.747880 | 5_2:+1984903 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTTACCAAACTGATCATTAC+TGG | + | chr5_2:1984943-1984962 | None:intergenic | 30.0% | |
| TTGTTCTGGTCATTGTATGA+AGG | - | chr5_2:1984691-1984710 | Msa0748720:CDS | 35.0% | |
| GAAGTACACAACCACATTTA+CGG | + | chr5_2:1984762-1984781 | None:intergenic | 35.0% | |
| ! | TACAACAACTTGGTAACATG+TGG | + | chr5_2:1984813-1984832 | None:intergenic | 35.0% |
| TGTTACCAAGTTGTTGTAAC+AGG | - | chr5_2:1984815-1984834 | Msa0748720:CDS | 35.0% | |
| ACAATCCTGTTACAACAACT+TGG | + | chr5_2:1984823-1984842 | None:intergenic | 35.0% | |
| ATCCTATCAAGAAACAACGT+AGG | - | chr5_2:1984848-1984867 | Msa0748720:CDS | 35.0% | |
| TTCTCCAGTAATGATCAGTT+TGG | - | chr5_2:1984936-1984955 | Msa0748720:CDS | 35.0% | |
| !! | TTGTATGAAGGAAGCATCTC+TGG | - | chr5_2:1984703-1984722 | Msa0748720:CDS | 40.0% |
| !! | CTCCTACGTTGTTTCTTGAT+AGG | + | chr5_2:1984853-1984872 | None:intergenic | 40.0% |
| CAAGAAACAACGTAGGAGTT+TGG | - | chr5_2:1984855-1984874 | Msa0748720:CDS | 40.0% | |
| ACTGGAGAAGAAGAATCAGT+TGG | + | chr5_2:1984925-1984944 | None:intergenic | 40.0% | |
| TTTGGTAAACCTTCAAGAGC+AGG | - | chr5_2:1984954-1984973 | Msa0748720:CDS | 40.0% | |
| AGACCTTACCACCGTAAATG+TGG | - | chr5_2:1984748-1984767 | Msa0748720:CDS | 45.0% | |
| CAACCACATTTACGGTGGTA+AGG | + | chr5_2:1984754-1984773 | None:intergenic | 45.0% | |
| GTACACAACCACATTTACGG+TGG | + | chr5_2:1984759-1984778 | None:intergenic | 45.0% | |
| ! | AGGAGGAGGAGATTGATTCA+AGG | - | chr5_2:1984974-1984993 | Msa0748720:CDS | 45.0% |
| ! | GGAGATTGATTCAAGGTCTG+AGG | - | chr5_2:1984981-1985000 | Msa0748720:CDS | 45.0% |
| GAACAATCCATCTCCAGCAG+TGG | + | chr5_2:1984677-1984696 | None:intergenic | 50.0% | |
| !! | CTGCTGGAGATGGATTGTTC+TGG | - | chr5_2:1984677-1984696 | Msa0748720:CDS | 50.0% |
| GGTAAACCTTCAAGAGCAGG+AGG | - | chr5_2:1984957-1984976 | Msa0748720:CDS | 50.0% | |
| AAACCTTCAAGAGCAGGAGG+AGG | - | chr5_2:1984960-1984979 | Msa0748720:CDS | 50.0% | |
| TCTCCTCCTCCTGCTCTTGA+AGG | + | chr5_2:1984966-1984985 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 1984670 | 1985011 | 1984670 | ID=Msa0748720;Name=Msa0748720 |
| chr5_2 | mRNA | 1984670 | 1985011 | 1984670 | ID=Msa0748720-mRNA-1;Parent=Msa0748720;Name=Msa0748720-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|-1|0|1|-1|1|1|0|113 |
| chr5_2 | exon | 1984670 | 1985011 | 1984670 | ID=Msa0748720-mRNA-1:exon:582;Parent=Msa0748720-mRNA-1 |
| chr5_2 | CDS | 1984670 | 1985011 | 1984670 | ID=Msa0748720-mRNA-1:cds;Parent=Msa0748720-mRNA-1 |
| Gene Sequence |
| Protein sequence |
