AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1226770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1226770 MtrunA17_Chr4g0061701 98.198 222 4 0 1 222 1 222 6.49e-164 451
Msa1226770 MtrunA17_Chr5g0420141 48.131 214 103 2 17 222 23 236 4.70e-66 204
Msa1226770 MtrunA17_Chr3g0114601 32.704 159 99 3 65 222 73 224 2.32e-23 93.6
Msa1226770 MtrunA17_Chr8g0380301 28.390 236 112 7 40 222 3 234 4.80e-19 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1226770 AT3G03290.1 51.163 172 80 2 55 222 103 274 5.62e-59 187
Msa1226770 AT5G17390.1 52.353 170 77 2 57 222 116 285 1.18e-57 184
Msa1226770 AT2G03720.2 47.087 206 98 8 26 222 29 232 5.25e-54 173
Msa1226770 AT2G03720.1 50.602 166 77 5 61 222 1 165 2.03e-50 162
Msa1226770 AT1G69080.1 43.284 201 99 3 37 222 23 223 7.34e-50 162
Msa1226770 AT1G69080.2 44.970 169 88 3 57 222 43 209 7.11e-43 144
Msa1226770 AT1G44760.1 37.805 164 88 3 59 222 64 213 3.66e-26 101
Msa1226770 AT1G44760.2 36.723 177 96 4 48 222 122 284 1.06e-25 101
Msa1226770 AT4G13450.1 30.516 213 90 8 59 222 16 219 1.66e-18 80.9

Find 53 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGTTCTGCATCTTCTTT+TGG 0.214542 8_2:+1780989 Msa1226770:CDS
TATGACTATATCAATGAAAT+TGG 0.241868 8_2:+1782464 Msa1226770:three_prime_UTR
GCAACAAAGGGTGTCTTTAT+TGG 0.266089 8_2:+1782244 Msa1226770:CDS
AAGCTAAAGGTGCTCTTGAT+TGG 0.333141 8_2:+1781128 Msa1226770:CDS
ACCCTTACTTTATGTGTATT+AGG 0.338897 8_2:-1782509 None:intergenic
ATACTCAGCAACCCCACTTT+TGG 0.340798 8_2:-1782330 None:intergenic
CACACATACTCTTCATATCT+AGG 0.373729 8_2:-1781393 None:intergenic
TGCCTAATACACATAAAGTA+AGG 0.393050 8_2:+1782507 Msa1226770:three_prime_UTR
ATTGCTTGAAGGGGAGGAAA+AGG 0.407520 8_2:+1782196 Msa1226770:CDS
AGCTAAAGGTGCTCTTGATT+GGG 0.425409 8_2:+1781129 Msa1226770:CDS
ACTTTGAACGAGCTTTCTTC+TGG 0.432468 8_2:+1782647 Msa1226770:three_prime_UTR
GAAATTGGTTATGGTGTTTG+TGG 0.432650 8_2:+1782479 Msa1226770:three_prime_UTR
AAGGGTGTCTTTATTGGTGG+TGG 0.462177 8_2:+1782250 Msa1226770:CDS
GTGAATATAGTATTGCTTGA+AGG 0.462359 8_2:+1782185 Msa1226770:CDS
TATATCAATGAAATTGGTTA+TGG 0.465159 8_2:+1782470 Msa1226770:three_prime_UTR
GAACAAGAACAACAGTATCT+TGG 0.472345 8_2:-1781174 None:intergenic
ATTCTTCCTCCTCACTGCAA+TGG 0.482834 8_2:-1782378 None:intergenic
TGAATATAGTATTGCTTGAA+GGG 0.488641 8_2:+1782186 Msa1226770:CDS
AAATTGGTTATGGTGTTTGT+GGG 0.506424 8_2:+1782480 Msa1226770:three_prime_UTR
ACTCTGGAAGAGATAACAAA+GGG 0.515139 8_2:+1781059 Msa1226770:CDS
GATTCTAGCTTTGAAGCTAA+AGG 0.523893 8_2:+1781115 Msa1226770:CDS
TATAGTATTGCTTGAAGGGG+AGG 0.536510 8_2:+1782190 Msa1226770:CDS
CTCACTGCAATGGTCATACA+AGG 0.537662 8_2:-1782368 None:intergenic
TGCAAGAGGCAAAGCAACAA+AGG 0.539284 8_2:+1782231 Msa1226770:CDS
GCAAGAGGCAAAGCAACAAA+GGG 0.539516 8_2:+1782232 Msa1226770:CDS
ACAAAGGGTGTCTTTATTGG+TGG 0.547210 8_2:+1782247 Msa1226770:CDS
AGCAACGGAGTTCAAGACTC+TGG 0.548078 8_2:+1781043 Msa1226770:CDS
CAATAAAGTGATGGTTGTTG+TGG 0.548385 8_2:+1781093 Msa1226770:CDS
TGTGGTGCTTGATGAGGAAG+TGG 0.561004 8_2:+1782294 Msa1226770:CDS
TTGCTTGAAGGGGAGGAAAA+GGG 0.567097 8_2:+1782197 Msa1226770:CDS
GATAACAAAGGGGATGAAAG+TGG 0.578843 8_2:+1781070 Msa1226770:CDS
TGATGTGAAGTTTAATCTGA+AGG 0.584331 8_2:+1781357 Msa1226770:CDS
GGGTGCTGCAATAGTGCAAG+AGG 0.588176 8_2:+1782217 Msa1226770:CDS
GAATCAATTAATCAAAGACA+TGG 0.589079 8_2:+1780904 Msa1226770:exon
AGGGTGTCTTTATTGGTGGT+GGG 0.591794 8_2:+1782251 Msa1226770:CDS
AGAAAGCTCGTTCAAAGTGA+AGG 0.595325 8_2:-1782642 None:intergenic
ATGTGTGAAATGAAGAAACC+TGG 0.596732 8_2:+1781409 Msa1226770:CDS
CAAGAAAGAGAACCAAAAGT+GGG 0.599249 8_2:+1782318 Msa1226770:CDS
ACAAGAAAGAGAACCAAAAG+TGG 0.608229 8_2:+1782317 Msa1226770:CDS
GTAGCTAGACCCCTCAGAGA+AGG 0.610978 8_2:+1781199 Msa1226770:CDS
AAGTTGCATAGTTTGTGCGT+TGG 0.613774 8_2:+1780941 Msa1226770:CDS
GCCTAATACACATAAAGTAA+GGG 0.626152 8_2:+1782508 Msa1226770:three_prime_UTR
GACTCTGGAAGAGATAACAA+AGG 0.639056 8_2:+1781058 Msa1226770:CDS
TGTGTGAAATGAAGAAACCT+GGG 0.642180 8_2:+1781410 Msa1226770:CDS
GTATGACCATTGCAGTGAGG+AGG 0.646086 8_2:+1782372 Msa1226770:CDS
GAATATAGTATTGCTTGAAG+GGG 0.648267 8_2:+1782187 Msa1226770:CDS
GCAGAACATGCTCGAACACG+AGG 0.664452 8_2:-1780977 None:intergenic
TGAAAGTGGCAATAAAGTGA+TGG 0.668312 8_2:+1781084 Msa1226770:CDS
AAACTATGCAACTTGAAACG+TGG 0.681932 8_2:-1780932 None:intergenic
CTTGTATGACCATTGCAGTG+AGG 0.716302 8_2:+1782369 Msa1226770:CDS
AAGAAAGAGAACCAAAAGTG+GGG 0.725592 8_2:+1782319 Msa1226770:CDS
CTCTGGAAGAGATAACAAAG+GGG 0.742102 8_2:+1781060 Msa1226770:CDS
GTGTGAAATGAAGAAACCTG+GGG 0.759265 8_2:+1781411 Msa1226770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTAAAATACATTTTAAAATT+TGG - chr8_2:1781840-1781859 None:intergenic 10.0%
!!! CTAAAATACATTTTAAAATT+TGG - chr8_2:1781840-1781859 None:intergenic 10.0%
!!! TTTCTTTCTAAAATTGTAAT+TGG + chr8_2:1781265-1781284 Msa1226770:intron 15.0%
!!! AAATGTATTTTAGATTTGTT+TGG + chr8_2:1781847-1781866 Msa1226770:intron 15.0%
!! TTCTCATTTATTTAATCAAA+TGG + chr8_2:1781909-1781928 Msa1226770:intron 15.0%
!! ATTACTAATAGCATAAATTA+TGG - chr8_2:1782012-1782031 None:intergenic 15.0%
!!! TTTCTTTCTAAAATTGTAAT+TGG + chr8_2:1781265-1781284 Msa1226770:intron 15.0%
!!! AAATGTATTTTAGATTTGTT+TGG + chr8_2:1781847-1781866 Msa1226770:intron 15.0%
!! TTCTCATTTATTTAATCAAA+TGG + chr8_2:1781909-1781928 Msa1226770:intron 15.0%
!! ATTACTAATAGCATAAATTA+TGG - chr8_2:1782012-1782031 None:intergenic 15.0%
!! TAATTAGAGTTATATCGTAT+TGG - chr8_2:1781605-1781624 None:intergenic 20.0%
!! CCAAAATTTCTCTTATAAAT+AGG - chr8_2:1781642-1781661 None:intergenic 20.0%
!!! CCTATTTATAAGAGAAATTT+TGG + chr8_2:1781639-1781658 Msa1226770:intron 20.0%
!! TCAACAAAAGTTAATGTATT+TGG + chr8_2:1781662-1781681 Msa1226770:intron 20.0%
!!! ATAAAACTGACTTTTAAATG+AGG - chr8_2:1781795-1781814 None:intergenic 20.0%
!! TATGACTATATCAATGAAAT+TGG + chr8_2:1782464-1782483 Msa1226770:three_prime_UTR 20.0%
!! TATATCAATGAAATTGGTTA+TGG + chr8_2:1782470-1782489 Msa1226770:three_prime_UTR 20.0%
!! TAATTAGAGTTATATCGTAT+TGG - chr8_2:1781605-1781624 None:intergenic 20.0%
!! CCAAAATTTCTCTTATAAAT+AGG - chr8_2:1781642-1781661 None:intergenic 20.0%
!!! CCTATTTATAAGAGAAATTT+TGG + chr8_2:1781639-1781658 Msa1226770:intron 20.0%
!! TCAACAAAAGTTAATGTATT+TGG + chr8_2:1781662-1781681 Msa1226770:intron 20.0%
!!! ATAAAACTGACTTTTAAATG+AGG - chr8_2:1781795-1781814 None:intergenic 20.0%
!! TATGACTATATCAATGAAAT+TGG + chr8_2:1782464-1782483 Msa1226770:three_prime_UTR 20.0%
!! TATATCAATGAAATTGGTTA+TGG + chr8_2:1782470-1782489 Msa1226770:three_prime_UTR 20.0%
! GAATCAATTAATCAAAGACA+TGG + chr8_2:1780904-1780923 Msa1226770:exon 25.0%
! TAATTGGACTAATGTGAAAA+GGG + chr8_2:1781281-1781300 Msa1226770:intron 25.0%
!!! TGTTCTATTGATTCATTACT+AGG + chr8_2:1781325-1781344 Msa1226770:intron 25.0%
!! TGTTACTTGATATTGTGTTA+AGG + chr8_2:1781468-1781487 Msa1226770:intron 25.0%
! GTTACTTGATATTGTGTTAA+GGG + chr8_2:1781469-1781488 Msa1226770:intron 25.0%
!!! TGAAATGACTCAGTTATTTT+GGG - chr8_2:1781557-1781576 None:intergenic 25.0%
!! TTGAAATGACTCAGTTATTT+TGG - chr8_2:1781558-1781577 None:intergenic 25.0%
! ATTTCTCTTATAAATAGGAC+CGG - chr8_2:1781637-1781656 None:intergenic 25.0%
! AATGTATTTGGTTAAAAACG+TGG + chr8_2:1781674-1781693 Msa1226770:intron 25.0%
! TCAACAAAAGTGAATGTATT+TGG - chr8_2:1781701-1781720 None:intergenic 25.0%
! TTTGGGTATAAGTTTATAAG+AGG + chr8_2:1781762-1781781 Msa1226770:intron 25.0%
! TGAATATAGTATTGCTTGAA+GGG + chr8_2:1782186-1782205 Msa1226770:CDS 25.0%
!! AAAGAAAACGTTCTATTTTG+TGG + chr8_2:1782276-1782295 Msa1226770:CDS 25.0%
! GGACAAAAATATTCAAGTAA+GGG - chr8_2:1782624-1782643 None:intergenic 25.0%
! AGGACAAAAATATTCAAGTA+AGG - chr8_2:1782625-1782644 None:intergenic 25.0%
!! AAATGTCGTGTATATTTTCT+AGG - chr8_2:1782688-1782707 None:intergenic 25.0%
! GAATCAATTAATCAAAGACA+TGG + chr8_2:1780904-1780923 Msa1226770:exon 25.0%
! TAATTGGACTAATGTGAAAA+GGG + chr8_2:1781281-1781300 Msa1226770:intron 25.0%
!!! TGTTCTATTGATTCATTACT+AGG + chr8_2:1781325-1781344 Msa1226770:intron 25.0%
!! TGTTACTTGATATTGTGTTA+AGG + chr8_2:1781468-1781487 Msa1226770:intron 25.0%
! GTTACTTGATATTGTGTTAA+GGG + chr8_2:1781469-1781488 Msa1226770:intron 25.0%
!!! TGAAATGACTCAGTTATTTT+GGG - chr8_2:1781557-1781576 None:intergenic 25.0%
!! TTGAAATGACTCAGTTATTT+TGG - chr8_2:1781558-1781577 None:intergenic 25.0%
! ATTTCTCTTATAAATAGGAC+CGG - chr8_2:1781637-1781656 None:intergenic 25.0%
! AATGTATTTGGTTAAAAACG+TGG + chr8_2:1781674-1781693 Msa1226770:intron 25.0%
! TCAACAAAAGTGAATGTATT+TGG - chr8_2:1781701-1781720 None:intergenic 25.0%
! TTTGGGTATAAGTTTATAAG+AGG + chr8_2:1781762-1781781 Msa1226770:intron 25.0%
! TGAATATAGTATTGCTTGAA+GGG + chr8_2:1782186-1782205 Msa1226770:CDS 25.0%
!! AAAGAAAACGTTCTATTTTG+TGG + chr8_2:1782276-1782295 Msa1226770:CDS 25.0%
! GGACAAAAATATTCAAGTAA+GGG - chr8_2:1782624-1782643 None:intergenic 25.0%
! AGGACAAAAATATTCAAGTA+AGG - chr8_2:1782625-1782644 None:intergenic 25.0%
!! AAATGTCGTGTATATTTTCT+AGG - chr8_2:1782688-1782707 None:intergenic 25.0%
GTAATTGGACTAATGTGAAA+AGG + chr8_2:1781280-1781299 Msa1226770:intron 30.0%
AATTGGACTAATGTGAAAAG+GGG + chr8_2:1781282-1781301 Msa1226770:intron 30.0%
TGATGTGAAGTTTAATCTGA+AGG + chr8_2:1781357-1781376 Msa1226770:CDS 30.0%
ACTCTAATTAATACTACCTC+CGG + chr8_2:1781615-1781634 Msa1226770:intron 30.0%
TATAAGTTTATAAGAGGAGG+CGG + chr8_2:1781768-1781787 Msa1226770:intron 30.0%
GTGAATATAGTATTGCTTGA+AGG + chr8_2:1782185-1782204 Msa1226770:CDS 30.0%
GAATATAGTATTGCTTGAAG+GGG + chr8_2:1782187-1782206 Msa1226770:CDS 30.0%
AGGAGGAAGAATAAAAAACT+TGG + chr8_2:1782389-1782408 Msa1226770:CDS 30.0%
AGGAAGAATAAAAAACTTGG+AGG + chr8_2:1782392-1782411 Msa1226770:CDS 30.0%
GGAAGAATAAAAAACTTGGA+GGG + chr8_2:1782393-1782412 Msa1226770:CDS 30.0%
! CCAAAAGCCAAAAGTTTTTA+TGG - chr8_2:1782439-1782458 None:intergenic 30.0%
!!! CCATAAAAACTTTTGGCTTT+TGG + chr8_2:1782436-1782455 Msa1226770:CDS 30.0%
AAATTGGTTATGGTGTTTGT+GGG + chr8_2:1782480-1782499 Msa1226770:three_prime_UTR 30.0%
TGCCTAATACACATAAAGTA+AGG + chr8_2:1782507-1782526 Msa1226770:three_prime_UTR 30.0%
GCCTAATACACATAAAGTAA+GGG + chr8_2:1782508-1782527 Msa1226770:three_prime_UTR 30.0%
ACCCTTACTTTATGTGTATT+AGG - chr8_2:1782512-1782531 None:intergenic 30.0%
GTAATTGGACTAATGTGAAA+AGG + chr8_2:1781280-1781299 Msa1226770:intron 30.0%
AATTGGACTAATGTGAAAAG+GGG + chr8_2:1781282-1781301 Msa1226770:intron 30.0%
TGATGTGAAGTTTAATCTGA+AGG + chr8_2:1781357-1781376 Msa1226770:CDS 30.0%
ACTCTAATTAATACTACCTC+CGG + chr8_2:1781615-1781634 Msa1226770:intron 30.0%
TATAAGTTTATAAGAGGAGG+CGG + chr8_2:1781768-1781787 Msa1226770:intron 30.0%
GTGAATATAGTATTGCTTGA+AGG + chr8_2:1782185-1782204 Msa1226770:CDS 30.0%
GAATATAGTATTGCTTGAAG+GGG + chr8_2:1782187-1782206 Msa1226770:CDS 30.0%
AGGAGGAAGAATAAAAAACT+TGG + chr8_2:1782389-1782408 Msa1226770:CDS 30.0%
AGGAAGAATAAAAAACTTGG+AGG + chr8_2:1782392-1782411 Msa1226770:CDS 30.0%
GGAAGAATAAAAAACTTGGA+GGG + chr8_2:1782393-1782412 Msa1226770:CDS 30.0%
! CCAAAAGCCAAAAGTTTTTA+TGG - chr8_2:1782439-1782458 None:intergenic 30.0%
!!! CCATAAAAACTTTTGGCTTT+TGG + chr8_2:1782436-1782455 Msa1226770:CDS 30.0%
AAATTGGTTATGGTGTTTGT+GGG + chr8_2:1782480-1782499 Msa1226770:three_prime_UTR 30.0%
TGCCTAATACACATAAAGTA+AGG + chr8_2:1782507-1782526 Msa1226770:three_prime_UTR 30.0%
GCCTAATACACATAAAGTAA+GGG + chr8_2:1782508-1782527 Msa1226770:three_prime_UTR 30.0%
ACCCTTACTTTATGTGTATT+AGG - chr8_2:1782512-1782531 None:intergenic 30.0%
AAACTATGCAACTTGAAACG+TGG - chr8_2:1780935-1780954 None:intergenic 35.0%
!! AGAACTGAGTTTTCAAGCAA+CGG + chr8_2:1781028-1781047 Msa1226770:CDS 35.0%
! ACTCTGGAAGAGATAACAAA+GGG + chr8_2:1781059-1781078 Msa1226770:CDS 35.0%
TGAAAGTGGCAATAAAGTGA+TGG + chr8_2:1781084-1781103 Msa1226770:CDS 35.0%
CAATAAAGTGATGGTTGTTG+TGG + chr8_2:1781093-1781112 Msa1226770:CDS 35.0%
!! GATTCTAGCTTTGAAGCTAA+AGG + chr8_2:1781115-1781134 Msa1226770:CDS 35.0%
GAACAAGAACAACAGTATCT+TGG - chr8_2:1781177-1781196 None:intergenic 35.0%
!!! TTTTTTCTGACCTTCTCTGA+GGG - chr8_2:1781212-1781231 None:intergenic 35.0%
CACACATACTCTTCATATCT+AGG - chr8_2:1781396-1781415 None:intergenic 35.0%
ATGTGTGAAATGAAGAAACC+TGG + chr8_2:1781409-1781428 Msa1226770:CDS 35.0%
TGTGTGAAATGAAGAAACCT+GGG + chr8_2:1781410-1781429 Msa1226770:CDS 35.0%
GGGTATAAGTTTATAAGAGG+AGG + chr8_2:1781765-1781784 Msa1226770:intron 35.0%
TACACACACACATATGCATA+TGG + chr8_2:1782130-1782149 Msa1226770:intron 35.0%
CATATGCATATGGTAACATG+TGG + chr8_2:1782140-1782159 Msa1226770:intron 35.0%
ATGGTAACATGTGGAGTTTA+TGG + chr8_2:1782149-1782168 Msa1226770:intron 35.0%
TGGTAACATGTGGAGTTTAT+GGG + chr8_2:1782150-1782169 Msa1226770:intron 35.0%
ACAAGAAAGAGAACCAAAAG+TGG + chr8_2:1782317-1782336 Msa1226770:CDS 35.0%
CAAGAAAGAGAACCAAAAGT+GGG + chr8_2:1782318-1782337 Msa1226770:CDS 35.0%
AAGAAAGAGAACCAAAAGTG+GGG + chr8_2:1782319-1782338 Msa1226770:CDS 35.0%
! CCAAAAGTTTTTATGGCGTT+TGG - chr8_2:1782432-1782451 None:intergenic 35.0%
! CCAAACGCCATAAAAACTTT+TGG + chr8_2:1782429-1782448 Msa1226770:CDS 35.0%
GAAATTGGTTATGGTGTTTG+TGG + chr8_2:1782479-1782498 Msa1226770:three_prime_UTR 35.0%
AAACTATGCAACTTGAAACG+TGG - chr8_2:1780935-1780954 None:intergenic 35.0%
!! AGAACTGAGTTTTCAAGCAA+CGG + chr8_2:1781028-1781047 Msa1226770:CDS 35.0%
! ACTCTGGAAGAGATAACAAA+GGG + chr8_2:1781059-1781078 Msa1226770:CDS 35.0%
TGAAAGTGGCAATAAAGTGA+TGG + chr8_2:1781084-1781103 Msa1226770:CDS 35.0%
CAATAAAGTGATGGTTGTTG+TGG + chr8_2:1781093-1781112 Msa1226770:CDS 35.0%
!! GATTCTAGCTTTGAAGCTAA+AGG + chr8_2:1781115-1781134 Msa1226770:CDS 35.0%
GAACAAGAACAACAGTATCT+TGG - chr8_2:1781177-1781196 None:intergenic 35.0%
!!! TTTTTTCTGACCTTCTCTGA+GGG - chr8_2:1781212-1781231 None:intergenic 35.0%
CACACATACTCTTCATATCT+AGG - chr8_2:1781396-1781415 None:intergenic 35.0%
ATGTGTGAAATGAAGAAACC+TGG + chr8_2:1781409-1781428 Msa1226770:CDS 35.0%
TGTGTGAAATGAAGAAACCT+GGG + chr8_2:1781410-1781429 Msa1226770:CDS 35.0%
GGGTATAAGTTTATAAGAGG+AGG + chr8_2:1781765-1781784 Msa1226770:intron 35.0%
TACACACACACATATGCATA+TGG + chr8_2:1782130-1782149 Msa1226770:intron 35.0%
CATATGCATATGGTAACATG+TGG + chr8_2:1782140-1782159 Msa1226770:intron 35.0%
ATGGTAACATGTGGAGTTTA+TGG + chr8_2:1782149-1782168 Msa1226770:intron 35.0%
TGGTAACATGTGGAGTTTAT+GGG + chr8_2:1782150-1782169 Msa1226770:intron 35.0%
ACAAGAAAGAGAACCAAAAG+TGG + chr8_2:1782317-1782336 Msa1226770:CDS 35.0%
CAAGAAAGAGAACCAAAAGT+GGG + chr8_2:1782318-1782337 Msa1226770:CDS 35.0%
AAGAAAGAGAACCAAAAGTG+GGG + chr8_2:1782319-1782338 Msa1226770:CDS 35.0%
! CCAAAAGTTTTTATGGCGTT+TGG - chr8_2:1782432-1782451 None:intergenic 35.0%
! CCAAACGCCATAAAAACTTT+TGG + chr8_2:1782429-1782448 Msa1226770:CDS 35.0%
GAAATTGGTTATGGTGTTTG+TGG + chr8_2:1782479-1782498 Msa1226770:three_prime_UTR 35.0%
! AAGTTGCATAGTTTGTGCGT+TGG + chr8_2:1780941-1780960 Msa1226770:CDS 40.0%
! GCATGTTCTGCATCTTCTTT+TGG + chr8_2:1780989-1781008 Msa1226770:CDS 40.0%
! GACTCTGGAAGAGATAACAA+AGG + chr8_2:1781058-1781077 Msa1226770:CDS 40.0%
! CTCTGGAAGAGATAACAAAG+GGG + chr8_2:1781060-1781079 Msa1226770:CDS 40.0%
GATAACAAAGGGGATGAAAG+TGG + chr8_2:1781070-1781089 Msa1226770:CDS 40.0%
!! AAGCTAAAGGTGCTCTTGAT+TGG + chr8_2:1781128-1781147 Msa1226770:CDS 40.0%
!! AGCTAAAGGTGCTCTTGATT+GGG + chr8_2:1781129-1781148 Msa1226770:CDS 40.0%
! TTTTTCTGACCTTCTCTGAG+GGG - chr8_2:1781211-1781230 None:intergenic 40.0%
!!! CTTTTTTCTGACCTTCTCTG+AGG - chr8_2:1781213-1781232 None:intergenic 40.0%
GTGTGAAATGAAGAAACCTG+GGG + chr8_2:1781411-1781430 Msa1226770:CDS 40.0%
CTTGTTTGAAAACTTACCCC+AGG - chr8_2:1781430-1781449 None:intergenic 40.0%
AAATGATGTTACCTCCGTCT+CGG + chr8_2:1781500-1781519 Msa1226770:intron 40.0%
ATGAGTCAGATATTCCGAGA+CGG - chr8_2:1781517-1781536 None:intergenic 40.0%
TCTCTTATAAATAGGACCGG+AGG - chr8_2:1781634-1781653 None:intergenic 40.0%
! ACGGAGGGAGTATATAGTTT+GGG + chr8_2:1781745-1781764 Msa1226770:intron 40.0%
TCAAATGGTTCGACTCATCA+CGG + chr8_2:1781924-1781943 Msa1226770:intron 40.0%
ATGTGGAGTTTATGGGTTTC+AGG + chr8_2:1782157-1782176 Msa1226770:intron 40.0%
TATAGTATTGCTTGAAGGGG+AGG + chr8_2:1782190-1782209 Msa1226770:CDS 40.0%
!! GCAACAAAGGGTGTCTTTAT+TGG + chr8_2:1782244-1782263 Msa1226770:CDS 40.0%
!! ACAAAGGGTGTCTTTATTGG+TGG + chr8_2:1782247-1782266 Msa1226770:CDS 40.0%
! CTATTTTGTGGTGCTTGATG+AGG + chr8_2:1782288-1782307 Msa1226770:CDS 40.0%
AGAAAGCTCGTTCAAAGTGA+AGG - chr8_2:1782645-1782664 None:intergenic 40.0%
ACTTTGAACGAGCTTTCTTC+TGG + chr8_2:1782647-1782666 Msa1226770:three_prime_UTR 40.0%
!! CGTGTATATTTTCTAGGAGC+AGG - chr8_2:1782682-1782701 None:intergenic 40.0%
! AAGTTGCATAGTTTGTGCGT+TGG + chr8_2:1780941-1780960 Msa1226770:CDS 40.0%
! GCATGTTCTGCATCTTCTTT+TGG + chr8_2:1780989-1781008 Msa1226770:CDS 40.0%
! GACTCTGGAAGAGATAACAA+AGG + chr8_2:1781058-1781077 Msa1226770:CDS 40.0%
! CTCTGGAAGAGATAACAAAG+GGG + chr8_2:1781060-1781079 Msa1226770:CDS 40.0%
GATAACAAAGGGGATGAAAG+TGG + chr8_2:1781070-1781089 Msa1226770:CDS 40.0%
!! AAGCTAAAGGTGCTCTTGAT+TGG + chr8_2:1781128-1781147 Msa1226770:CDS 40.0%
!! AGCTAAAGGTGCTCTTGATT+GGG + chr8_2:1781129-1781148 Msa1226770:CDS 40.0%
! TTTTTCTGACCTTCTCTGAG+GGG - chr8_2:1781211-1781230 None:intergenic 40.0%
!!! CTTTTTTCTGACCTTCTCTG+AGG - chr8_2:1781213-1781232 None:intergenic 40.0%
GTGTGAAATGAAGAAACCTG+GGG + chr8_2:1781411-1781430 Msa1226770:CDS 40.0%
CTTGTTTGAAAACTTACCCC+AGG - chr8_2:1781430-1781449 None:intergenic 40.0%
AAATGATGTTACCTCCGTCT+CGG + chr8_2:1781500-1781519 Msa1226770:intron 40.0%
ATGAGTCAGATATTCCGAGA+CGG - chr8_2:1781517-1781536 None:intergenic 40.0%
TCTCTTATAAATAGGACCGG+AGG - chr8_2:1781634-1781653 None:intergenic 40.0%
! ACGGAGGGAGTATATAGTTT+GGG + chr8_2:1781745-1781764 Msa1226770:intron 40.0%
TCAAATGGTTCGACTCATCA+CGG + chr8_2:1781924-1781943 Msa1226770:intron 40.0%
ATGTGGAGTTTATGGGTTTC+AGG + chr8_2:1782157-1782176 Msa1226770:intron 40.0%
TATAGTATTGCTTGAAGGGG+AGG + chr8_2:1782190-1782209 Msa1226770:CDS 40.0%
!! GCAACAAAGGGTGTCTTTAT+TGG + chr8_2:1782244-1782263 Msa1226770:CDS 40.0%
!! ACAAAGGGTGTCTTTATTGG+TGG + chr8_2:1782247-1782266 Msa1226770:CDS 40.0%
! CTATTTTGTGGTGCTTGATG+AGG + chr8_2:1782288-1782307 Msa1226770:CDS 40.0%
AGAAAGCTCGTTCAAAGTGA+AGG - chr8_2:1782645-1782664 None:intergenic 40.0%
ACTTTGAACGAGCTTTCTTC+TGG + chr8_2:1782647-1782666 Msa1226770:three_prime_UTR 40.0%
!! CGTGTATATTTTCTAGGAGC+AGG - chr8_2:1782682-1782701 None:intergenic 40.0%
! CGCAACTGACTCAGATATAC+CGG + chr8_2:1781721-1781740 Msa1226770:intron 45.0%
! GCAACTGACTCAGATATACC+GGG + chr8_2:1781722-1781741 Msa1226770:intron 45.0%
! GACGGAGGGAGTATATAGTT+TGG + chr8_2:1781744-1781763 Msa1226770:intron 45.0%
ATTGCTTGAAGGGGAGGAAA+AGG + chr8_2:1782196-1782215 Msa1226770:CDS 45.0%
TTGCTTGAAGGGGAGGAAAA+GGG + chr8_2:1782197-1782216 Msa1226770:CDS 45.0%
TGCAAGAGGCAAAGCAACAA+AGG + chr8_2:1782231-1782250 Msa1226770:CDS 45.0%
GCAAGAGGCAAAGCAACAAA+GGG + chr8_2:1782232-1782251 Msa1226770:CDS 45.0%
!! AAGGGTGTCTTTATTGGTGG+TGG + chr8_2:1782250-1782269 Msa1226770:CDS 45.0%
! AGGGTGTCTTTATTGGTGGT+GGG + chr8_2:1782251-1782270 Msa1226770:CDS 45.0%
! ATACTCAGCAACCCCACTTT+TGG - chr8_2:1782333-1782352 None:intergenic 45.0%
CTCACTGCAATGGTCATACA+AGG - chr8_2:1782371-1782390 None:intergenic 45.0%
CTTGTATGACCATTGCAGTG+AGG + chr8_2:1782369-1782388 Msa1226770:CDS 45.0%
ATTCTTCCTCCTCACTGCAA+TGG - chr8_2:1782381-1782400 None:intergenic 45.0%
! CGCAACTGACTCAGATATAC+CGG + chr8_2:1781721-1781740 Msa1226770:intron 45.0%
! GCAACTGACTCAGATATACC+GGG + chr8_2:1781722-1781741 Msa1226770:intron 45.0%
! GACGGAGGGAGTATATAGTT+TGG + chr8_2:1781744-1781763 Msa1226770:intron 45.0%
ATTGCTTGAAGGGGAGGAAA+AGG + chr8_2:1782196-1782215 Msa1226770:CDS 45.0%
TTGCTTGAAGGGGAGGAAAA+GGG + chr8_2:1782197-1782216 Msa1226770:CDS 45.0%
TGCAAGAGGCAAAGCAACAA+AGG + chr8_2:1782231-1782250 Msa1226770:CDS 45.0%
GCAAGAGGCAAAGCAACAAA+GGG + chr8_2:1782232-1782251 Msa1226770:CDS 45.0%
!! AAGGGTGTCTTTATTGGTGG+TGG + chr8_2:1782250-1782269 Msa1226770:CDS 45.0%
! AGGGTGTCTTTATTGGTGGT+GGG + chr8_2:1782251-1782270 Msa1226770:CDS 45.0%
! ATACTCAGCAACCCCACTTT+TGG - chr8_2:1782333-1782352 None:intergenic 45.0%
CTCACTGCAATGGTCATACA+AGG - chr8_2:1782371-1782390 None:intergenic 45.0%
CTTGTATGACCATTGCAGTG+AGG + chr8_2:1782369-1782388 Msa1226770:CDS 45.0%
ATTCTTCCTCCTCACTGCAA+TGG - chr8_2:1782381-1782400 None:intergenic 45.0%
!!! TAAAATACATTTTAAAATTT+GGG - chr8_2:1781839-1781858 None:intergenic 5.0%
!!! TAATTATTTTAATTAATCTA+TGG - chr8_2:1782059-1782078 None:intergenic 5.0%
!!! TAAAATACATTTTAAAATTT+GGG - chr8_2:1781839-1781858 None:intergenic 5.0%
!!! TAATTATTTTAATTAATCTA+TGG - chr8_2:1782059-1782078 None:intergenic 5.0%
AGCAACGGAGTTCAAGACTC+TGG + chr8_2:1781043-1781062 Msa1226770:CDS 50.0%
AGTCAGATATTCCGAGACGG+AGG - chr8_2:1781514-1781533 None:intergenic 50.0%
CTGACTCAGATATACCGGGA+CGG + chr8_2:1781726-1781745 Msa1226770:intron 50.0%
ACTATATACTCCCTCCGTCC+CGG - chr8_2:1781743-1781762 None:intergenic 50.0%
GAGAGCGGTGACTCAATAGT+AGG - chr8_2:1781878-1781897 None:intergenic 50.0%
AGAAGTCTTTGCCTCGAGAG+CGG - chr8_2:1781893-1781912 None:intergenic 50.0%
TGTGGTGCTTGATGAGGAAG+TGG + chr8_2:1782294-1782313 Msa1226770:CDS 50.0%
GTATGACCATTGCAGTGAGG+AGG + chr8_2:1782372-1782391 Msa1226770:CDS 50.0%
AGCAACGGAGTTCAAGACTC+TGG + chr8_2:1781043-1781062 Msa1226770:CDS 50.0%
AGTCAGATATTCCGAGACGG+AGG - chr8_2:1781514-1781533 None:intergenic 50.0%
CTGACTCAGATATACCGGGA+CGG + chr8_2:1781726-1781745 Msa1226770:intron 50.0%
ACTATATACTCCCTCCGTCC+CGG - chr8_2:1781743-1781762 None:intergenic 50.0%
GAGAGCGGTGACTCAATAGT+AGG - chr8_2:1781878-1781897 None:intergenic 50.0%
AGAAGTCTTTGCCTCGAGAG+CGG - chr8_2:1781893-1781912 None:intergenic 50.0%
TGTGGTGCTTGATGAGGAAG+TGG + chr8_2:1782294-1782313 Msa1226770:CDS 50.0%
GTATGACCATTGCAGTGAGG+AGG + chr8_2:1782372-1782391 Msa1226770:CDS 50.0%
GCAGAACATGCTCGAACACG+AGG - chr8_2:1780980-1780999 None:intergenic 55.0%
GTAGCTAGACCCCTCAGAGA+AGG + chr8_2:1781199-1781218 Msa1226770:CDS 55.0%
ACTCAGATATACCGGGACGG+AGG + chr8_2:1781729-1781748 Msa1226770:intron 55.0%
CTCAGATATACCGGGACGGA+GGG + chr8_2:1781730-1781749 Msa1226770:intron 55.0%
CTATTGAGTCACCGCTCTCG+AGG + chr8_2:1781879-1781898 Msa1226770:intron 55.0%
! GGGTGCTGCAATAGTGCAAG+AGG + chr8_2:1782217-1782236 Msa1226770:CDS 55.0%
GCAGAACATGCTCGAACACG+AGG - chr8_2:1780980-1780999 None:intergenic 55.0%
GTAGCTAGACCCCTCAGAGA+AGG + chr8_2:1781199-1781218 Msa1226770:CDS 55.0%
ACTCAGATATACCGGGACGG+AGG + chr8_2:1781729-1781748 Msa1226770:intron 55.0%
CTCAGATATACCGGGACGGA+GGG + chr8_2:1781730-1781749 Msa1226770:intron 55.0%
CTATTGAGTCACCGCTCTCG+AGG + chr8_2:1781879-1781898 Msa1226770:intron 55.0%
! GGGTGCTGCAATAGTGCAAG+AGG + chr8_2:1782217-1782236 Msa1226770:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 1780888 1782708 1780888 ID=Msa1226770;Name=Msa1226770
chr8_2 mRNA 1780888 1782708 1780888 ID=Msa1226770-mRNA-1;Parent=Msa1226770;Name=Msa1226770-mRNA-1;_AED=0.07;_eAED=0.07;_QI=35|1|1|1|1|1|3|245|222
chr8_2 exon 1780888 1781220 1780888 ID=Msa1226770-mRNA-1:exon:331;Parent=Msa1226770-mRNA-1
chr8_2 exon 1781347 1781432 1781347 ID=Msa1226770-mRNA-1:exon:332;Parent=Msa1226770-mRNA-1
chr8_2 exon 1782179 1782708 1782179 ID=Msa1226770-mRNA-1:exon:333;Parent=Msa1226770-mRNA-1
chr8_2 five_prime_UTR 1780888 1780922 1780888 ID=Msa1226770-mRNA-1:five_prime_utr;Parent=Msa1226770-mRNA-1
chr8_2 CDS 1780923 1781220 1780923 ID=Msa1226770-mRNA-1:cds;Parent=Msa1226770-mRNA-1
chr8_2 CDS 1781347 1781432 1781347 ID=Msa1226770-mRNA-1:cds;Parent=Msa1226770-mRNA-1
chr8_2 CDS 1782179 1782463 1782179 ID=Msa1226770-mRNA-1:cds;Parent=Msa1226770-mRNA-1
chr8_2 three_prime_UTR 1782464 1782708 1782464 ID=Msa1226770-mRNA-1:three_prime_utr;Parent=Msa1226770-mRNA-1
Gene Sequence

>Msa1226770

ATGGTTAGACCACGTTTCAAGTTGCATAGTTTGTGCGTTGGTCATGCTAGAACACCTCGTGTTCGAGCATGTTCTGCATCTTCTTTTGGATCAAAATTTGCAAACAGAACTGAGTTTTCAAGCAACGGAGTTCAAGACTCTGGAAGAGATAACAAAGGGGATGAAAGTGGCAATAAAGTGATGGTTGTTGTGGATTCTAGCTTTGAAGCTAAAGGTGCTCTTGATTGGGCACTCTCACACACTATTCAGAGCCAAGATACTGTTGTTCTTGTTCATGTAGCTAGACCCCTCAGAGAAGGCGACGAGTCTGATGTGAAGTTTAATCTGAAGGCATATCAACTTCTCCTAGATATGAAGAGTATGTGTGAAATGAAGAAACCTGGGGTGCTAGTGAATATAGTATTGCTTGAAGGGGAGGAAAAGGGTGCTGCAATAGTGCAAGAGGCAAAGCAACAAAGGGTGTCTTTATTGGTGGTGGGACAAAGAAAACGTTCTATTTTGTGGTGCTTGATGAGGAAGTGGACAAGAAAGAGAACCAAAAGTGGGGTTGCTGAGTATTGTATACAGAACTCACCTTGTATGACCATTGCAGTGAGGAGGAAGAATAAAAAACTTGGAGGGTATTTGATTACTACCAAACGCCATAAAAACTTTTGGCTTTTGGCTTAG
Protein sequence

>Msa1226770

MVRPRFKLHSLCVGHARTPRVRACSASSFGSKFANRTEFSSNGVQDSGRDNKGDESGNKVMVVVDSSFEAKGALDWALSHTIQSQDTVVLVHVARPLREGDESDVKFNLKAYQLLLDMKSMCEMKKPGVLVNIVLLEGEEKGAAIVQEAKQQRVSLLVVGQRKRSILWCLMRKWTRKRTKSGVAEYCIQNSPCMTIAVRRKNKKLGGYLITTKRHKNFWLLA*