AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1300230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1300230 MtrunA17_Chr4g0061701 98.198 222 4 0 1 222 1 222 6.49e-164 451
Msa1300230 MtrunA17_Chr5g0420141 48.131 214 103 2 17 222 23 236 4.70e-66 204
Msa1300230 MtrunA17_Chr3g0114601 32.704 159 99 3 65 222 73 224 2.32e-23 93.6
Msa1300230 MtrunA17_Chr8g0380301 28.390 236 112 7 40 222 3 234 4.80e-19 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1300230 AT3G03290.1 51.163 172 80 2 55 222 103 274 5.62e-59 187
Msa1300230 AT5G17390.1 52.353 170 77 2 57 222 116 285 1.18e-57 184
Msa1300230 AT2G03720.2 47.087 206 98 8 26 222 29 232 5.25e-54 173
Msa1300230 AT2G03720.1 50.602 166 77 5 61 222 1 165 2.03e-50 162
Msa1300230 AT1G69080.1 43.284 201 99 3 37 222 23 223 7.34e-50 162
Msa1300230 AT1G69080.2 44.970 169 88 3 57 222 43 209 7.11e-43 144
Msa1300230 AT1G44760.1 37.805 164 88 3 59 222 64 213 3.66e-26 101
Msa1300230 AT1G44760.2 36.723 177 96 4 48 222 122 284 1.06e-25 101
Msa1300230 AT4G13450.1 30.516 213 90 8 59 222 16 219 1.66e-18 80.9

Find 53 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGTTCTGCATCTTCTTT+TGG 0.214542 8_3:+61707631 Msa1300230:CDS
TATGACTATATCAATGAAAT+TGG 0.241868 8_3:+61709122 Msa1300230:three_prime_UTR
GCAACAAAGGGTGTCTTTAT+TGG 0.266089 8_3:+61708902 Msa1300230:CDS
AAGCTAAAGGTGCTCTTGAT+TGG 0.333141 8_3:+61707770 Msa1300230:CDS
ACCCTTACTTTATGTGTATT+AGG 0.338897 8_3:-61709167 None:intergenic
ATACTCAGCAACCCCACTTT+TGG 0.340798 8_3:-61708988 None:intergenic
CACACATACTCTTCATATCT+AGG 0.373729 8_3:-61708035 None:intergenic
TGCCTAATACACATAAAGTA+AGG 0.393050 8_3:+61709165 Msa1300230:three_prime_UTR
ATTGCTTGAAGGGGAGGAAA+AGG 0.407520 8_3:+61708854 Msa1300230:CDS
AGCTAAAGGTGCTCTTGATT+GGG 0.425409 8_3:+61707771 Msa1300230:CDS
ACTTTGAACGAGCTTTCTTC+TGG 0.432468 8_3:+61709305 Msa1300230:three_prime_UTR
GAAATTGGTTATGGTGTTTG+TGG 0.432650 8_3:+61709137 Msa1300230:three_prime_UTR
AAGGGTGTCTTTATTGGTGG+TGG 0.462177 8_3:+61708908 Msa1300230:CDS
GTGAATATAGTATTGCTTGA+AGG 0.462359 8_3:+61708843 Msa1300230:CDS
TATATCAATGAAATTGGTTA+TGG 0.465159 8_3:+61709128 Msa1300230:three_prime_UTR
GAACAAGAACAACAGTATCT+TGG 0.472345 8_3:-61707816 None:intergenic
ATTCTTCCTCCTCACTGCAA+TGG 0.482834 8_3:-61709036 None:intergenic
TGAATATAGTATTGCTTGAA+GGG 0.488641 8_3:+61708844 Msa1300230:CDS
AAATTGGTTATGGTGTTTGT+GGG 0.506424 8_3:+61709138 Msa1300230:three_prime_UTR
ACTCTGGAAGAGATAACAAA+GGG 0.515139 8_3:+61707701 Msa1300230:CDS
GATTCTAGCTTTGAAGCTAA+AGG 0.523893 8_3:+61707757 Msa1300230:CDS
TATAGTATTGCTTGAAGGGG+AGG 0.536510 8_3:+61708848 Msa1300230:CDS
CTCACTGCAATGGTCATACA+AGG 0.537662 8_3:-61709026 None:intergenic
TGCAAGAGGCAAAGCAACAA+AGG 0.539284 8_3:+61708889 Msa1300230:CDS
GCAAGAGGCAAAGCAACAAA+GGG 0.539516 8_3:+61708890 Msa1300230:CDS
ACAAAGGGTGTCTTTATTGG+TGG 0.547210 8_3:+61708905 Msa1300230:CDS
AGCAACGGAGTTCAAGACTC+TGG 0.548078 8_3:+61707685 Msa1300230:CDS
CAATAAAGTGATGGTTGTTG+TGG 0.548385 8_3:+61707735 Msa1300230:CDS
TGTGGTGCTTGATGAGGAAG+TGG 0.561004 8_3:+61708952 Msa1300230:CDS
TTGCTTGAAGGGGAGGAAAA+GGG 0.567097 8_3:+61708855 Msa1300230:CDS
GATAACAAAGGGGATGAAAG+TGG 0.578843 8_3:+61707712 Msa1300230:CDS
TGATGTGAAGTTTAATCTGA+AGG 0.584331 8_3:+61707999 Msa1300230:CDS
GGGTGCTGCAATAGTGCAAG+AGG 0.588176 8_3:+61708875 Msa1300230:CDS
GAATCAATTAATCAAAGACA+TGG 0.589079 8_3:+61707546 Msa1300230:exon
AGGGTGTCTTTATTGGTGGT+GGG 0.591794 8_3:+61708909 Msa1300230:CDS
AGAAAGCTCGTTCAAAGTGA+AGG 0.595325 8_3:-61709300 None:intergenic
ATGTGTGAAATGAAGAAACC+TGG 0.596732 8_3:+61708051 Msa1300230:CDS
CAAGAAAGAGAACCAAAAGT+GGG 0.599249 8_3:+61708976 Msa1300230:CDS
ACAAGAAAGAGAACCAAAAG+TGG 0.608229 8_3:+61708975 Msa1300230:CDS
GTAGCTAGACCCCTCAGAGA+AGG 0.610978 8_3:+61707841 Msa1300230:CDS
AAGTTGCATAGTTTGTGCGT+TGG 0.613774 8_3:+61707583 Msa1300230:CDS
GCCTAATACACATAAAGTAA+GGG 0.626152 8_3:+61709166 Msa1300230:three_prime_UTR
GACTCTGGAAGAGATAACAA+AGG 0.639056 8_3:+61707700 Msa1300230:CDS
TGTGTGAAATGAAGAAACCT+GGG 0.642180 8_3:+61708052 Msa1300230:CDS
GTATGACCATTGCAGTGAGG+AGG 0.646086 8_3:+61709030 Msa1300230:CDS
GAATATAGTATTGCTTGAAG+GGG 0.648267 8_3:+61708845 Msa1300230:CDS
GCAGAACATGCTCGAACACG+AGG 0.664452 8_3:-61707619 None:intergenic
TGAAAGTGGCAATAAAGTGA+TGG 0.668312 8_3:+61707726 Msa1300230:CDS
AAACTATGCAACTTGAAACG+TGG 0.681932 8_3:-61707574 None:intergenic
CTTGTATGACCATTGCAGTG+AGG 0.716302 8_3:+61709027 Msa1300230:CDS
AAGAAAGAGAACCAAAAGTG+GGG 0.725592 8_3:+61708977 Msa1300230:CDS
CTCTGGAAGAGATAACAAAG+GGG 0.742102 8_3:+61707702 Msa1300230:CDS
GTGTGAAATGAAGAAACCTG+GGG 0.759265 8_3:+61708053 Msa1300230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTAAAATACATTTTAAAATT+TGG - chr8_3:61708482-61708501 None:intergenic 10.0%
!!! CTAAAATACATTTTAAAATT+TGG - chr8_3:61708482-61708501 None:intergenic 10.0%
!!! TTTCTTTCTAAAATTGTAAT+TGG + chr8_3:61707907-61707926 Msa1300230:intron 15.0%
!! TAATTAGAGTTATATTGTAT+TGG - chr8_3:61708247-61708266 None:intergenic 15.0%
!!! AAATGTATTTTAGATTTGTT+TGG + chr8_3:61708489-61708508 Msa1300230:intron 15.0%
!! TTCTCATTTATTTAATCAAA+TGG + chr8_3:61708551-61708570 Msa1300230:intron 15.0%
!! ATTACTAATAGCATAAATTA+TGG - chr8_3:61708654-61708673 None:intergenic 15.0%
!!! TTTCTTTCTAAAATTGTAAT+TGG + chr8_3:61707907-61707926 Msa1300230:intron 15.0%
!! TAATTAGAGTTATATTGTAT+TGG - chr8_3:61708247-61708266 None:intergenic 15.0%
!!! AAATGTATTTTAGATTTGTT+TGG + chr8_3:61708489-61708508 Msa1300230:intron 15.0%
!! TTCTCATTTATTTAATCAAA+TGG + chr8_3:61708551-61708570 Msa1300230:intron 15.0%
!! ATTACTAATAGCATAAATTA+TGG - chr8_3:61708654-61708673 None:intergenic 15.0%
!! CCAAAATTTCTCTTATAAAT+AGG - chr8_3:61708284-61708303 None:intergenic 20.0%
!!! CCTATTTATAAGAGAAATTT+TGG + chr8_3:61708281-61708300 Msa1300230:intron 20.0%
!! TCAACAAAAGTTAATGTATT+TGG + chr8_3:61708304-61708323 Msa1300230:intron 20.0%
!!! ATAAAACTGACTTTTAAATG+AGG - chr8_3:61708437-61708456 None:intergenic 20.0%
!! TATGACTATATCAATGAAAT+TGG + chr8_3:61709122-61709141 Msa1300230:three_prime_UTR 20.0%
!! TATATCAATGAAATTGGTTA+TGG + chr8_3:61709128-61709147 Msa1300230:three_prime_UTR 20.0%
!! CCAAAATTTCTCTTATAAAT+AGG - chr8_3:61708284-61708303 None:intergenic 20.0%
!!! CCTATTTATAAGAGAAATTT+TGG + chr8_3:61708281-61708300 Msa1300230:intron 20.0%
!! TCAACAAAAGTTAATGTATT+TGG + chr8_3:61708304-61708323 Msa1300230:intron 20.0%
!!! ATAAAACTGACTTTTAAATG+AGG - chr8_3:61708437-61708456 None:intergenic 20.0%
!! TATGACTATATCAATGAAAT+TGG + chr8_3:61709122-61709141 Msa1300230:three_prime_UTR 20.0%
!! TATATCAATGAAATTGGTTA+TGG + chr8_3:61709128-61709147 Msa1300230:three_prime_UTR 20.0%
! GAATCAATTAATCAAAGACA+TGG + chr8_3:61707546-61707565 Msa1300230:exon 25.0%
! TAATTGGACTAATGTGAAAA+GGG + chr8_3:61707923-61707942 Msa1300230:intron 25.0%
!!! TGTTCTATTGATTCATTACT+AGG + chr8_3:61707967-61707986 Msa1300230:intron 25.0%
!! TGTTACTTGATATTGTGTTA+AGG + chr8_3:61708110-61708129 Msa1300230:intron 25.0%
! GTTACTTGATATTGTGTTAA+GGG + chr8_3:61708111-61708130 Msa1300230:intron 25.0%
!!! TGAAATGACTCAGTTATTTT+GGG - chr8_3:61708199-61708218 None:intergenic 25.0%
!! TTGAAATGACTCAGTTATTT+TGG - chr8_3:61708200-61708219 None:intergenic 25.0%
! ATTTCTCTTATAAATAGGAC+CGG - chr8_3:61708279-61708298 None:intergenic 25.0%
! AATGTATTTGGTTAAAAACG+TGG + chr8_3:61708316-61708335 Msa1300230:intron 25.0%
! TCAACAAAAGTGAATGTATT+TGG - chr8_3:61708343-61708362 None:intergenic 25.0%
! TTTGGGTATAAGTTTATAAG+AGG + chr8_3:61708404-61708423 Msa1300230:intron 25.0%
! TGAATATAGTATTGCTTGAA+GGG + chr8_3:61708844-61708863 Msa1300230:CDS 25.0%
!! AAAGAAAACGTTCTATTTTG+TGG + chr8_3:61708934-61708953 Msa1300230:CDS 25.0%
! GGACAAAAATATTCAAGTAA+GGG - chr8_3:61709282-61709301 None:intergenic 25.0%
! AGGACAAAAATATTCAAGTA+AGG - chr8_3:61709283-61709302 None:intergenic 25.0%
!! AAATGTCGTGTATATTTTCT+AGG - chr8_3:61709346-61709365 None:intergenic 25.0%
! GAATCAATTAATCAAAGACA+TGG + chr8_3:61707546-61707565 Msa1300230:exon 25.0%
! TAATTGGACTAATGTGAAAA+GGG + chr8_3:61707923-61707942 Msa1300230:intron 25.0%
!!! TGTTCTATTGATTCATTACT+AGG + chr8_3:61707967-61707986 Msa1300230:intron 25.0%
!! TGTTACTTGATATTGTGTTA+AGG + chr8_3:61708110-61708129 Msa1300230:intron 25.0%
! GTTACTTGATATTGTGTTAA+GGG + chr8_3:61708111-61708130 Msa1300230:intron 25.0%
!!! TGAAATGACTCAGTTATTTT+GGG - chr8_3:61708199-61708218 None:intergenic 25.0%
!! TTGAAATGACTCAGTTATTT+TGG - chr8_3:61708200-61708219 None:intergenic 25.0%
! ATTTCTCTTATAAATAGGAC+CGG - chr8_3:61708279-61708298 None:intergenic 25.0%
! AATGTATTTGGTTAAAAACG+TGG + chr8_3:61708316-61708335 Msa1300230:intron 25.0%
! TCAACAAAAGTGAATGTATT+TGG - chr8_3:61708343-61708362 None:intergenic 25.0%
! TTTGGGTATAAGTTTATAAG+AGG + chr8_3:61708404-61708423 Msa1300230:intron 25.0%
! TGAATATAGTATTGCTTGAA+GGG + chr8_3:61708844-61708863 Msa1300230:CDS 25.0%
!! AAAGAAAACGTTCTATTTTG+TGG + chr8_3:61708934-61708953 Msa1300230:CDS 25.0%
! GGACAAAAATATTCAAGTAA+GGG - chr8_3:61709282-61709301 None:intergenic 25.0%
! AGGACAAAAATATTCAAGTA+AGG - chr8_3:61709283-61709302 None:intergenic 25.0%
!! AAATGTCGTGTATATTTTCT+AGG - chr8_3:61709346-61709365 None:intergenic 25.0%
GTAATTGGACTAATGTGAAA+AGG + chr8_3:61707922-61707941 Msa1300230:intron 30.0%
AATTGGACTAATGTGAAAAG+GGG + chr8_3:61707924-61707943 Msa1300230:intron 30.0%
TGATGTGAAGTTTAATCTGA+AGG + chr8_3:61707999-61708018 Msa1300230:CDS 30.0%
ACTCTAATTAATACTACCTC+CGG + chr8_3:61708257-61708276 Msa1300230:intron 30.0%
TATAAGTTTATAAGAGGAGG+CGG + chr8_3:61708410-61708429 Msa1300230:intron 30.0%
GTGAATATAGTATTGCTTGA+AGG + chr8_3:61708843-61708862 Msa1300230:CDS 30.0%
GAATATAGTATTGCTTGAAG+GGG + chr8_3:61708845-61708864 Msa1300230:CDS 30.0%
AGGAGGAAGAATAAAAAACT+TGG + chr8_3:61709047-61709066 Msa1300230:CDS 30.0%
AGGAAGAATAAAAAACTTGG+AGG + chr8_3:61709050-61709069 Msa1300230:CDS 30.0%
GGAAGAATAAAAAACTTGGA+GGG + chr8_3:61709051-61709070 Msa1300230:CDS 30.0%
! CCAAAAGCCAAAAGTTTTTA+TGG - chr8_3:61709097-61709116 None:intergenic 30.0%
!!! CCATAAAAACTTTTGGCTTT+TGG + chr8_3:61709094-61709113 Msa1300230:CDS 30.0%
AAATTGGTTATGGTGTTTGT+GGG + chr8_3:61709138-61709157 Msa1300230:three_prime_UTR 30.0%
TGCCTAATACACATAAAGTA+AGG + chr8_3:61709165-61709184 Msa1300230:three_prime_UTR 30.0%
GCCTAATACACATAAAGTAA+GGG + chr8_3:61709166-61709185 Msa1300230:three_prime_UTR 30.0%
ACCCTTACTTTATGTGTATT+AGG - chr8_3:61709170-61709189 None:intergenic 30.0%
GTAATTGGACTAATGTGAAA+AGG + chr8_3:61707922-61707941 Msa1300230:intron 30.0%
AATTGGACTAATGTGAAAAG+GGG + chr8_3:61707924-61707943 Msa1300230:intron 30.0%
TGATGTGAAGTTTAATCTGA+AGG + chr8_3:61707999-61708018 Msa1300230:CDS 30.0%
ACTCTAATTAATACTACCTC+CGG + chr8_3:61708257-61708276 Msa1300230:intron 30.0%
TATAAGTTTATAAGAGGAGG+CGG + chr8_3:61708410-61708429 Msa1300230:intron 30.0%
GTGAATATAGTATTGCTTGA+AGG + chr8_3:61708843-61708862 Msa1300230:CDS 30.0%
GAATATAGTATTGCTTGAAG+GGG + chr8_3:61708845-61708864 Msa1300230:CDS 30.0%
AGGAGGAAGAATAAAAAACT+TGG + chr8_3:61709047-61709066 Msa1300230:CDS 30.0%
AGGAAGAATAAAAAACTTGG+AGG + chr8_3:61709050-61709069 Msa1300230:CDS 30.0%
GGAAGAATAAAAAACTTGGA+GGG + chr8_3:61709051-61709070 Msa1300230:CDS 30.0%
! CCAAAAGCCAAAAGTTTTTA+TGG - chr8_3:61709097-61709116 None:intergenic 30.0%
!!! CCATAAAAACTTTTGGCTTT+TGG + chr8_3:61709094-61709113 Msa1300230:CDS 30.0%
AAATTGGTTATGGTGTTTGT+GGG + chr8_3:61709138-61709157 Msa1300230:three_prime_UTR 30.0%
TGCCTAATACACATAAAGTA+AGG + chr8_3:61709165-61709184 Msa1300230:three_prime_UTR 30.0%
GCCTAATACACATAAAGTAA+GGG + chr8_3:61709166-61709185 Msa1300230:three_prime_UTR 30.0%
ACCCTTACTTTATGTGTATT+AGG - chr8_3:61709170-61709189 None:intergenic 30.0%
AAACTATGCAACTTGAAACG+TGG - chr8_3:61707577-61707596 None:intergenic 35.0%
!! AGAACTGAGTTTTCAAGCAA+CGG + chr8_3:61707670-61707689 Msa1300230:CDS 35.0%
! ACTCTGGAAGAGATAACAAA+GGG + chr8_3:61707701-61707720 Msa1300230:CDS 35.0%
TGAAAGTGGCAATAAAGTGA+TGG + chr8_3:61707726-61707745 Msa1300230:CDS 35.0%
CAATAAAGTGATGGTTGTTG+TGG + chr8_3:61707735-61707754 Msa1300230:CDS 35.0%
!! GATTCTAGCTTTGAAGCTAA+AGG + chr8_3:61707757-61707776 Msa1300230:CDS 35.0%
GAACAAGAACAACAGTATCT+TGG - chr8_3:61707819-61707838 None:intergenic 35.0%
!!! TTTTTTCTGACCTTCTCTGA+GGG - chr8_3:61707854-61707873 None:intergenic 35.0%
CACACATACTCTTCATATCT+AGG - chr8_3:61708038-61708057 None:intergenic 35.0%
ATGTGTGAAATGAAGAAACC+TGG + chr8_3:61708051-61708070 Msa1300230:CDS 35.0%
TGTGTGAAATGAAGAAACCT+GGG + chr8_3:61708052-61708071 Msa1300230:CDS 35.0%
GGGTATAAGTTTATAAGAGG+AGG + chr8_3:61708407-61708426 Msa1300230:intron 35.0%
CATATGCATATGGTAACATG+TGG + chr8_3:61708798-61708817 Msa1300230:intron 35.0%
ATGGTAACATGTGGAGTTTA+TGG + chr8_3:61708807-61708826 Msa1300230:intron 35.0%
TGGTAACATGTGGAGTTTAT+GGG + chr8_3:61708808-61708827 Msa1300230:intron 35.0%
ACAAGAAAGAGAACCAAAAG+TGG + chr8_3:61708975-61708994 Msa1300230:CDS 35.0%
CAAGAAAGAGAACCAAAAGT+GGG + chr8_3:61708976-61708995 Msa1300230:CDS 35.0%
AAGAAAGAGAACCAAAAGTG+GGG + chr8_3:61708977-61708996 Msa1300230:CDS 35.0%
! CCAAAAGTTTTTATGGCGTT+TGG - chr8_3:61709090-61709109 None:intergenic 35.0%
! CCAAACGCCATAAAAACTTT+TGG + chr8_3:61709087-61709106 Msa1300230:CDS 35.0%
GAAATTGGTTATGGTGTTTG+TGG + chr8_3:61709137-61709156 Msa1300230:three_prime_UTR 35.0%
AAACTATGCAACTTGAAACG+TGG - chr8_3:61707577-61707596 None:intergenic 35.0%
!! AGAACTGAGTTTTCAAGCAA+CGG + chr8_3:61707670-61707689 Msa1300230:CDS 35.0%
! ACTCTGGAAGAGATAACAAA+GGG + chr8_3:61707701-61707720 Msa1300230:CDS 35.0%
TGAAAGTGGCAATAAAGTGA+TGG + chr8_3:61707726-61707745 Msa1300230:CDS 35.0%
CAATAAAGTGATGGTTGTTG+TGG + chr8_3:61707735-61707754 Msa1300230:CDS 35.0%
!! GATTCTAGCTTTGAAGCTAA+AGG + chr8_3:61707757-61707776 Msa1300230:CDS 35.0%
GAACAAGAACAACAGTATCT+TGG - chr8_3:61707819-61707838 None:intergenic 35.0%
!!! TTTTTTCTGACCTTCTCTGA+GGG - chr8_3:61707854-61707873 None:intergenic 35.0%
CACACATACTCTTCATATCT+AGG - chr8_3:61708038-61708057 None:intergenic 35.0%
ATGTGTGAAATGAAGAAACC+TGG + chr8_3:61708051-61708070 Msa1300230:CDS 35.0%
TGTGTGAAATGAAGAAACCT+GGG + chr8_3:61708052-61708071 Msa1300230:CDS 35.0%
GGGTATAAGTTTATAAGAGG+AGG + chr8_3:61708407-61708426 Msa1300230:intron 35.0%
CATATGCATATGGTAACATG+TGG + chr8_3:61708798-61708817 Msa1300230:intron 35.0%
ATGGTAACATGTGGAGTTTA+TGG + chr8_3:61708807-61708826 Msa1300230:intron 35.0%
TGGTAACATGTGGAGTTTAT+GGG + chr8_3:61708808-61708827 Msa1300230:intron 35.0%
ACAAGAAAGAGAACCAAAAG+TGG + chr8_3:61708975-61708994 Msa1300230:CDS 35.0%
CAAGAAAGAGAACCAAAAGT+GGG + chr8_3:61708976-61708995 Msa1300230:CDS 35.0%
AAGAAAGAGAACCAAAAGTG+GGG + chr8_3:61708977-61708996 Msa1300230:CDS 35.0%
! CCAAAAGTTTTTATGGCGTT+TGG - chr8_3:61709090-61709109 None:intergenic 35.0%
! CCAAACGCCATAAAAACTTT+TGG + chr8_3:61709087-61709106 Msa1300230:CDS 35.0%
GAAATTGGTTATGGTGTTTG+TGG + chr8_3:61709137-61709156 Msa1300230:three_prime_UTR 35.0%
! AAGTTGCATAGTTTGTGCGT+TGG + chr8_3:61707583-61707602 Msa1300230:CDS 40.0%
! GCATGTTCTGCATCTTCTTT+TGG + chr8_3:61707631-61707650 Msa1300230:CDS 40.0%
! GACTCTGGAAGAGATAACAA+AGG + chr8_3:61707700-61707719 Msa1300230:CDS 40.0%
! CTCTGGAAGAGATAACAAAG+GGG + chr8_3:61707702-61707721 Msa1300230:CDS 40.0%
GATAACAAAGGGGATGAAAG+TGG + chr8_3:61707712-61707731 Msa1300230:CDS 40.0%
!! AAGCTAAAGGTGCTCTTGAT+TGG + chr8_3:61707770-61707789 Msa1300230:CDS 40.0%
!! AGCTAAAGGTGCTCTTGATT+GGG + chr8_3:61707771-61707790 Msa1300230:CDS 40.0%
! TTTTTCTGACCTTCTCTGAG+GGG - chr8_3:61707853-61707872 None:intergenic 40.0%
!!! CTTTTTTCTGACCTTCTCTG+AGG - chr8_3:61707855-61707874 None:intergenic 40.0%
GTGTGAAATGAAGAAACCTG+GGG + chr8_3:61708053-61708072 Msa1300230:CDS 40.0%
CTTGTTTGAAAACTTACCCC+AGG - chr8_3:61708072-61708091 None:intergenic 40.0%
AAATGATGTTACCTCCGTCT+CGG + chr8_3:61708142-61708161 Msa1300230:intron 40.0%
ATGAGTCAGATATTCCGAGA+CGG - chr8_3:61708159-61708178 None:intergenic 40.0%
TCTCTTATAAATAGGACCGG+AGG - chr8_3:61708276-61708295 None:intergenic 40.0%
! ACGGAGGGAGTATATAGTTT+GGG + chr8_3:61708387-61708406 Msa1300230:intron 40.0%
CACACACACACATATGCATA+TGG + chr8_3:61708788-61708807 Msa1300230:intron 40.0%
ATGTGGAGTTTATGGGTTTC+AGG + chr8_3:61708815-61708834 Msa1300230:intron 40.0%
TATAGTATTGCTTGAAGGGG+AGG + chr8_3:61708848-61708867 Msa1300230:CDS 40.0%
!! GCAACAAAGGGTGTCTTTAT+TGG + chr8_3:61708902-61708921 Msa1300230:CDS 40.0%
!! ACAAAGGGTGTCTTTATTGG+TGG + chr8_3:61708905-61708924 Msa1300230:CDS 40.0%
! CTATTTTGTGGTGCTTGATG+AGG + chr8_3:61708946-61708965 Msa1300230:CDS 40.0%
AGAAAGCTCGTTCAAAGTGA+AGG - chr8_3:61709303-61709322 None:intergenic 40.0%
ACTTTGAACGAGCTTTCTTC+TGG + chr8_3:61709305-61709324 Msa1300230:three_prime_UTR 40.0%
!! CGTGTATATTTTCTAGGAGC+AGG - chr8_3:61709340-61709359 None:intergenic 40.0%
! AAGTTGCATAGTTTGTGCGT+TGG + chr8_3:61707583-61707602 Msa1300230:CDS 40.0%
! GCATGTTCTGCATCTTCTTT+TGG + chr8_3:61707631-61707650 Msa1300230:CDS 40.0%
! GACTCTGGAAGAGATAACAA+AGG + chr8_3:61707700-61707719 Msa1300230:CDS 40.0%
! CTCTGGAAGAGATAACAAAG+GGG + chr8_3:61707702-61707721 Msa1300230:CDS 40.0%
GATAACAAAGGGGATGAAAG+TGG + chr8_3:61707712-61707731 Msa1300230:CDS 40.0%
!! AAGCTAAAGGTGCTCTTGAT+TGG + chr8_3:61707770-61707789 Msa1300230:CDS 40.0%
!! AGCTAAAGGTGCTCTTGATT+GGG + chr8_3:61707771-61707790 Msa1300230:CDS 40.0%
! TTTTTCTGACCTTCTCTGAG+GGG - chr8_3:61707853-61707872 None:intergenic 40.0%
!!! CTTTTTTCTGACCTTCTCTG+AGG - chr8_3:61707855-61707874 None:intergenic 40.0%
GTGTGAAATGAAGAAACCTG+GGG + chr8_3:61708053-61708072 Msa1300230:CDS 40.0%
CTTGTTTGAAAACTTACCCC+AGG - chr8_3:61708072-61708091 None:intergenic 40.0%
AAATGATGTTACCTCCGTCT+CGG + chr8_3:61708142-61708161 Msa1300230:intron 40.0%
ATGAGTCAGATATTCCGAGA+CGG - chr8_3:61708159-61708178 None:intergenic 40.0%
TCTCTTATAAATAGGACCGG+AGG - chr8_3:61708276-61708295 None:intergenic 40.0%
! ACGGAGGGAGTATATAGTTT+GGG + chr8_3:61708387-61708406 Msa1300230:intron 40.0%
CACACACACACATATGCATA+TGG + chr8_3:61708788-61708807 Msa1300230:intron 40.0%
ATGTGGAGTTTATGGGTTTC+AGG + chr8_3:61708815-61708834 Msa1300230:intron 40.0%
TATAGTATTGCTTGAAGGGG+AGG + chr8_3:61708848-61708867 Msa1300230:CDS 40.0%
!! GCAACAAAGGGTGTCTTTAT+TGG + chr8_3:61708902-61708921 Msa1300230:CDS 40.0%
!! ACAAAGGGTGTCTTTATTGG+TGG + chr8_3:61708905-61708924 Msa1300230:CDS 40.0%
! CTATTTTGTGGTGCTTGATG+AGG + chr8_3:61708946-61708965 Msa1300230:CDS 40.0%
AGAAAGCTCGTTCAAAGTGA+AGG - chr8_3:61709303-61709322 None:intergenic 40.0%
ACTTTGAACGAGCTTTCTTC+TGG + chr8_3:61709305-61709324 Msa1300230:three_prime_UTR 40.0%
!! CGTGTATATTTTCTAGGAGC+AGG - chr8_3:61709340-61709359 None:intergenic 40.0%
! CGCAACTGACTCAGATATAC+CGG + chr8_3:61708363-61708382 Msa1300230:intron 45.0%
! GCAACTGACTCAGATATACC+GGG + chr8_3:61708364-61708383 Msa1300230:intron 45.0%
! GACGGAGGGAGTATATAGTT+TGG + chr8_3:61708386-61708405 Msa1300230:intron 45.0%
ATTGCTTGAAGGGGAGGAAA+AGG + chr8_3:61708854-61708873 Msa1300230:CDS 45.0%
TTGCTTGAAGGGGAGGAAAA+GGG + chr8_3:61708855-61708874 Msa1300230:CDS 45.0%
TGCAAGAGGCAAAGCAACAA+AGG + chr8_3:61708889-61708908 Msa1300230:CDS 45.0%
GCAAGAGGCAAAGCAACAAA+GGG + chr8_3:61708890-61708909 Msa1300230:CDS 45.0%
!! AAGGGTGTCTTTATTGGTGG+TGG + chr8_3:61708908-61708927 Msa1300230:CDS 45.0%
! AGGGTGTCTTTATTGGTGGT+GGG + chr8_3:61708909-61708928 Msa1300230:CDS 45.0%
! ATACTCAGCAACCCCACTTT+TGG - chr8_3:61708991-61709010 None:intergenic 45.0%
CTCACTGCAATGGTCATACA+AGG - chr8_3:61709029-61709048 None:intergenic 45.0%
CTTGTATGACCATTGCAGTG+AGG + chr8_3:61709027-61709046 Msa1300230:CDS 45.0%
ATTCTTCCTCCTCACTGCAA+TGG - chr8_3:61709039-61709058 None:intergenic 45.0%
! CGCAACTGACTCAGATATAC+CGG + chr8_3:61708363-61708382 Msa1300230:intron 45.0%
! GCAACTGACTCAGATATACC+GGG + chr8_3:61708364-61708383 Msa1300230:intron 45.0%
! GACGGAGGGAGTATATAGTT+TGG + chr8_3:61708386-61708405 Msa1300230:intron 45.0%
ATTGCTTGAAGGGGAGGAAA+AGG + chr8_3:61708854-61708873 Msa1300230:CDS 45.0%
TTGCTTGAAGGGGAGGAAAA+GGG + chr8_3:61708855-61708874 Msa1300230:CDS 45.0%
TGCAAGAGGCAAAGCAACAA+AGG + chr8_3:61708889-61708908 Msa1300230:CDS 45.0%
GCAAGAGGCAAAGCAACAAA+GGG + chr8_3:61708890-61708909 Msa1300230:CDS 45.0%
!! AAGGGTGTCTTTATTGGTGG+TGG + chr8_3:61708908-61708927 Msa1300230:CDS 45.0%
! AGGGTGTCTTTATTGGTGGT+GGG + chr8_3:61708909-61708928 Msa1300230:CDS 45.0%
! ATACTCAGCAACCCCACTTT+TGG - chr8_3:61708991-61709010 None:intergenic 45.0%
CTCACTGCAATGGTCATACA+AGG - chr8_3:61709029-61709048 None:intergenic 45.0%
CTTGTATGACCATTGCAGTG+AGG + chr8_3:61709027-61709046 Msa1300230:CDS 45.0%
ATTCTTCCTCCTCACTGCAA+TGG - chr8_3:61709039-61709058 None:intergenic 45.0%
!!! TAAAATACATTTTAAAATTT+GGG - chr8_3:61708481-61708500 None:intergenic 5.0%
!!! TAATTATTTTAATTAATCTA+TGG - chr8_3:61708701-61708720 None:intergenic 5.0%
!!! TAAAATACATTTTAAAATTT+GGG - chr8_3:61708481-61708500 None:intergenic 5.0%
!!! TAATTATTTTAATTAATCTA+TGG - chr8_3:61708701-61708720 None:intergenic 5.0%
AGCAACGGAGTTCAAGACTC+TGG + chr8_3:61707685-61707704 Msa1300230:CDS 50.0%
AGTCAGATATTCCGAGACGG+AGG - chr8_3:61708156-61708175 None:intergenic 50.0%
CTGACTCAGATATACCGGGA+CGG + chr8_3:61708368-61708387 Msa1300230:intron 50.0%
ACTATATACTCCCTCCGTCC+CGG - chr8_3:61708385-61708404 None:intergenic 50.0%
GAGAGCGGTGACTCAATAGT+AGG - chr8_3:61708520-61708539 None:intergenic 50.0%
AGAAGTCTTTGCCTCGAGAG+CGG - chr8_3:61708535-61708554 None:intergenic 50.0%
TGTGGTGCTTGATGAGGAAG+TGG + chr8_3:61708952-61708971 Msa1300230:CDS 50.0%
GTATGACCATTGCAGTGAGG+AGG + chr8_3:61709030-61709049 Msa1300230:CDS 50.0%
AGCAACGGAGTTCAAGACTC+TGG + chr8_3:61707685-61707704 Msa1300230:CDS 50.0%
AGTCAGATATTCCGAGACGG+AGG - chr8_3:61708156-61708175 None:intergenic 50.0%
CTGACTCAGATATACCGGGA+CGG + chr8_3:61708368-61708387 Msa1300230:intron 50.0%
ACTATATACTCCCTCCGTCC+CGG - chr8_3:61708385-61708404 None:intergenic 50.0%
GAGAGCGGTGACTCAATAGT+AGG - chr8_3:61708520-61708539 None:intergenic 50.0%
AGAAGTCTTTGCCTCGAGAG+CGG - chr8_3:61708535-61708554 None:intergenic 50.0%
TGTGGTGCTTGATGAGGAAG+TGG + chr8_3:61708952-61708971 Msa1300230:CDS 50.0%
GTATGACCATTGCAGTGAGG+AGG + chr8_3:61709030-61709049 Msa1300230:CDS 50.0%
GCAGAACATGCTCGAACACG+AGG - chr8_3:61707622-61707641 None:intergenic 55.0%
GTAGCTAGACCCCTCAGAGA+AGG + chr8_3:61707841-61707860 Msa1300230:CDS 55.0%
ACTCAGATATACCGGGACGG+AGG + chr8_3:61708371-61708390 Msa1300230:intron 55.0%
CTCAGATATACCGGGACGGA+GGG + chr8_3:61708372-61708391 Msa1300230:intron 55.0%
CTATTGAGTCACCGCTCTCG+AGG + chr8_3:61708521-61708540 Msa1300230:intron 55.0%
! GGGTGCTGCAATAGTGCAAG+AGG + chr8_3:61708875-61708894 Msa1300230:CDS 55.0%
GCAGAACATGCTCGAACACG+AGG - chr8_3:61707622-61707641 None:intergenic 55.0%
GTAGCTAGACCCCTCAGAGA+AGG + chr8_3:61707841-61707860 Msa1300230:CDS 55.0%
ACTCAGATATACCGGGACGG+AGG + chr8_3:61708371-61708390 Msa1300230:intron 55.0%
CTCAGATATACCGGGACGGA+GGG + chr8_3:61708372-61708391 Msa1300230:intron 55.0%
CTATTGAGTCACCGCTCTCG+AGG + chr8_3:61708521-61708540 Msa1300230:intron 55.0%
! GGGTGCTGCAATAGTGCAAG+AGG + chr8_3:61708875-61708894 Msa1300230:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 61707530 61709366 61707530 ID=Msa1300230;Name=Msa1300230
chr8_3 mRNA 61707530 61709366 61707530 ID=Msa1300230-mRNA-1;Parent=Msa1300230;Name=Msa1300230-mRNA-1;_AED=0.07;_eAED=0.07;_QI=35|1|1|1|1|1|3|245|222
chr8_3 exon 61707530 61707862 61707530 ID=Msa1300230-mRNA-1:exon:18239;Parent=Msa1300230-mRNA-1
chr8_3 exon 61707989 61708074 61707989 ID=Msa1300230-mRNA-1:exon:18240;Parent=Msa1300230-mRNA-1
chr8_3 exon 61708837 61709366 61708837 ID=Msa1300230-mRNA-1:exon:18241;Parent=Msa1300230-mRNA-1
chr8_3 five_prime_UTR 61707530 61707564 61707530 ID=Msa1300230-mRNA-1:five_prime_utr;Parent=Msa1300230-mRNA-1
chr8_3 CDS 61707565 61707862 61707565 ID=Msa1300230-mRNA-1:cds;Parent=Msa1300230-mRNA-1
chr8_3 CDS 61707989 61708074 61707989 ID=Msa1300230-mRNA-1:cds;Parent=Msa1300230-mRNA-1
chr8_3 CDS 61708837 61709121 61708837 ID=Msa1300230-mRNA-1:cds;Parent=Msa1300230-mRNA-1
chr8_3 three_prime_UTR 61709122 61709366 61709122 ID=Msa1300230-mRNA-1:three_prime_utr;Parent=Msa1300230-mRNA-1
Gene Sequence

>Msa1300230

ATGGTTAGACCACGTTTCAAGTTGCATAGTTTGTGCGTTGGTCATGCTAGAACACCTCGTGTTCGAGCATGTTCTGCATCTTCTTTTGGATCAAAATTTGCAAACAGAACTGAGTTTTCAAGCAACGGAGTTCAAGACTCTGGAAGAGATAACAAAGGGGATGAAAGTGGCAATAAAGTGATGGTTGTTGTGGATTCTAGCTTTGAAGCTAAAGGTGCTCTTGATTGGGCACTCTCACACACTATTCAGAGCCAAGATACTGTTGTTCTTGTTCATGTAGCTAGACCCCTCAGAGAAGGCGACGAGTCTGATGTGAAGTTTAATCTGAAGGCATATCAACTTCTCCTAGATATGAAGAGTATGTGTGAAATGAAGAAACCTGGGGTGCTAGTGAATATAGTATTGCTTGAAGGGGAGGAAAAGGGTGCTGCAATAGTGCAAGAGGCAAAGCAACAAAGGGTGTCTTTATTGGTGGTGGGACAAAGAAAACGTTCTATTTTGTGGTGCTTGATGAGGAAGTGGACAAGAAAGAGAACCAAAAGTGGGGTTGCTGAGTATTGTATACAGAACTCACCTTGTATGACCATTGCAGTGAGGAGGAAGAATAAAAAACTTGGAGGGTATTTGATTACTACCAAACGCCATAAAAACTTTTGGCTTTTGGCTTAG
Protein sequence

>Msa1300230

MVRPRFKLHSLCVGHARTPRVRACSASSFGSKFANRTEFSSNGVQDSGRDNKGDESGNKVMVVVDSSFEAKGALDWALSHTIQSQDTVVLVHVARPLREGDESDVKFNLKAYQLLLDMKSMCEMKKPGVLVNIVLLEGEEKGAAIVQEAKQQRVSLLVVGQRKRSILWCLMRKWTRKRTKSGVAEYCIQNSPCMTIAVRRKNKKLGGYLITTKRHKNFWLLA*