AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1213250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1213250 MtrunA17_Chr4g0061701 98.198 222 4 0 1 222 1 222 6.49e-164 451
Msa1213250 MtrunA17_Chr5g0420141 48.131 214 103 2 17 222 23 236 4.70e-66 204
Msa1213250 MtrunA17_Chr3g0114601 32.704 159 99 3 65 222 73 224 2.32e-23 93.6
Msa1213250 MtrunA17_Chr8g0380301 28.390 236 112 7 40 222 3 234 4.80e-19 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1213250 AT3G03290.1 51.163 172 80 2 55 222 103 274 5.62e-59 187
Msa1213250 AT5G17390.1 52.353 170 77 2 57 222 116 285 1.18e-57 184
Msa1213250 AT2G03720.2 47.087 206 98 8 26 222 29 232 5.25e-54 173
Msa1213250 AT2G03720.1 50.602 166 77 5 61 222 1 165 2.03e-50 162
Msa1213250 AT1G69080.1 43.284 201 99 3 37 222 23 223 7.34e-50 162
Msa1213250 AT1G69080.2 44.970 169 88 3 57 222 43 209 7.11e-43 144
Msa1213250 AT1G44760.1 37.805 164 88 3 59 222 64 213 3.66e-26 101
Msa1213250 AT1G44760.2 36.723 177 96 4 48 222 122 284 1.06e-25 101
Msa1213250 AT4G13450.1 30.516 213 90 8 59 222 16 219 1.66e-18 80.9

Find 57 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGTTCTGCATCTTCTTT+TGG 0.214542 8_1:+73384529 Msa1213250:CDS
TTCCCCTTGCTTGAATATTT+TGG 0.216579 8_1:+73386209 Msa1213250:three_prime_UTR
TATGACTATATCAATGAAAT+TGG 0.241868 8_1:+73386054 Msa1213250:three_prime_UTR
GCAACAAAGGGTGTCTTTAT+TGG 0.266089 8_1:+73385834 Msa1213250:CDS
TTCAAGTTCAAATTATTAAC+TGG 0.296871 8_1:+73386171 Msa1213250:three_prime_UTR
AAGCTAAAGGTGCTCTTGAT+TGG 0.333141 8_1:+73384668 Msa1213250:CDS
ACCCTTACTTTATGTGTATT+AGG 0.338897 8_1:-73386099 None:intergenic
ATACTCAGCAACCCCACTTT+TGG 0.340798 8_1:-73385920 None:intergenic
CACACATACTCTTCATATCT+AGG 0.373729 8_1:-73384931 None:intergenic
TGCCTAATACACATAAAGTA+AGG 0.393050 8_1:+73386097 Msa1213250:three_prime_UTR
ATTGCTTGAAGGGGAGGAAA+AGG 0.407520 8_1:+73385786 Msa1213250:CDS
AGCTAAAGGTGCTCTTGATT+GGG 0.425409 8_1:+73384669 Msa1213250:CDS
AAGGGTGTCTTTATTGGTGG+TGG 0.462177 8_1:+73385840 Msa1213250:CDS
GTGAATATAGTATTGCTTGA+AGG 0.462359 8_1:+73385775 Msa1213250:CDS
TATATCAATGAAATTGGTTA+TGG 0.465159 8_1:+73386060 Msa1213250:three_prime_UTR
GAACAAGAACAACAGTATCT+TGG 0.472345 8_1:-73384714 None:intergenic
ATTCTTCCTCCTCACTGCAA+TGG 0.482834 8_1:-73385968 None:intergenic
TGAATATAGTATTGCTTGAA+GGG 0.488641 8_1:+73385776 Msa1213250:CDS
ACTCTGGAAGAGATAACAAA+GGG 0.515139 8_1:+73384599 Msa1213250:CDS
GATTCTAGCTTTGAAGCTAA+AGG 0.523893 8_1:+73384655 Msa1213250:CDS
TATAGTATTGCTTGAAGGGG+AGG 0.536510 8_1:+73385780 Msa1213250:CDS
CTCACTGCAATGGTCATACA+AGG 0.537662 8_1:-73385958 None:intergenic
TGCAAGAGGCAAAGCAACAA+AGG 0.539284 8_1:+73385821 Msa1213250:CDS
GCAAGAGGCAAAGCAACAAA+GGG 0.539516 8_1:+73385822 Msa1213250:CDS
ACAAAGGGTGTCTTTATTGG+TGG 0.547210 8_1:+73385837 Msa1213250:CDS
AGCAACGGAGTTCAAGACTC+TGG 0.548078 8_1:+73384583 Msa1213250:CDS
CAATAAAGTGATGGTTGTTG+TGG 0.548385 8_1:+73384633 Msa1213250:CDS
TGTGGTGCTTGATGAGGAAG+TGG 0.561004 8_1:+73385884 Msa1213250:CDS
GAAATTGGTTATGGTGTCTG+TGG 0.564250 8_1:+73386069 Msa1213250:three_prime_UTR
TTGCTTGAAGGGGAGGAAAA+GGG 0.567097 8_1:+73385787 Msa1213250:CDS
GGACCAAAATATTCAAGCAA+GGG 0.574049 8_1:-73386212 None:intergenic
GATAACAAAGGGGATGAAAG+TGG 0.578843 8_1:+73384610 Msa1213250:CDS
TGATGTGAAGTTTAATCTGA+AGG 0.584331 8_1:+73384895 Msa1213250:CDS
AAATTGGTTATGGTGTCTGT+GGG 0.588143 8_1:+73386070 Msa1213250:three_prime_UTR
GGGTGCTGCAATAGTGCAAG+AGG 0.588176 8_1:+73385807 Msa1213250:CDS
GAATCAATTAATCAAAGACA+TGG 0.589079 8_1:+73384444 Msa1213250:exon
AGGGTGTCTTTATTGGTGGT+GGG 0.591794 8_1:+73385841 Msa1213250:CDS
AGAAAGCTCGTTCAAAGTGA+AGG 0.595325 8_1:-73386233 None:intergenic
ATGTGTGAAATGAAGAAACC+TGG 0.596732 8_1:+73384947 Msa1213250:CDS
CAAGAAAGAGAACCAAAAGT+GGG 0.599249 8_1:+73385908 Msa1213250:CDS
ACAAGAAAGAGAACCAAAAG+TGG 0.608229 8_1:+73385907 Msa1213250:CDS
GTAGCTAGACCCCTCAGAGA+AGG 0.610978 8_1:+73384739 Msa1213250:CDS
AAGTTGCATAGTTTGTGCGT+TGG 0.613774 8_1:+73384481 Msa1213250:CDS
AGGACCAAAATATTCAAGCA+AGG 0.616892 8_1:-73386213 None:intergenic
GACCAAAATATTCAAGCAAG+GGG 0.625465 8_1:-73386211 None:intergenic
GCCTAATACACATAAAGTAA+GGG 0.626152 8_1:+73386098 Msa1213250:three_prime_UTR
GACTCTGGAAGAGATAACAA+AGG 0.639056 8_1:+73384598 Msa1213250:CDS
TGTGTGAAATGAAGAAACCT+GGG 0.642180 8_1:+73384948 Msa1213250:CDS
GTATGACCATTGCAGTGAGG+AGG 0.646086 8_1:+73385962 Msa1213250:CDS
GAATATAGTATTGCTTGAAG+GGG 0.648267 8_1:+73385777 Msa1213250:CDS
GCAGAACATGCTCGAACACG+AGG 0.664452 8_1:-73384517 None:intergenic
TGAAAGTGGCAATAAAGTGA+TGG 0.668312 8_1:+73384624 Msa1213250:CDS
AAACTATGCAACTTGAAACG+TGG 0.681932 8_1:-73384472 None:intergenic
CTTGTATGACCATTGCAGTG+AGG 0.716302 8_1:+73385959 Msa1213250:CDS
AAGAAAGAGAACCAAAAGTG+GGG 0.725592 8_1:+73385909 Msa1213250:CDS
CTCTGGAAGAGATAACAAAG+GGG 0.742102 8_1:+73384600 Msa1213250:CDS
GTGTGAAATGAAGAAACCTG+GGG 0.759265 8_1:+73384949 Msa1213250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAT+AGG + chr8_1:73385396-73385415 Msa1213250:intron 0.0%
!! TATATATATATATATATATA+GGG + chr8_1:73385397-73385416 Msa1213250:intron 0.0%
!! ATATATATATATATATATAT+AGG + chr8_1:73385396-73385415 Msa1213250:intron 0.0%
!! TATATATATATATATATATA+GGG + chr8_1:73385397-73385416 Msa1213250:intron 0.0%
!!! CTAAAATACATTTTAAAATT+TGG - chr8_1:73385129-73385148 None:intergenic 10.0%
!! TGTCTATATATATATATATA+GGG - chr8_1:73385710-73385729 None:intergenic 10.0%
!!! CTAAAATACATTTTAAAATT+TGG - chr8_1:73385129-73385148 None:intergenic 10.0%
!! TGTCTATATATATATATATA+GGG - chr8_1:73385710-73385729 None:intergenic 10.0%
!!! TTTTTTCTTTCTATTGTAAT+TGG + chr8_1:73384803-73384822 Msa1213250:intron 15.0%
!!! AAATGTATTTTAGATTTGTT+TGG + chr8_1:73385136-73385155 Msa1213250:intron 15.0%
!! TTCTCATTTATTTAATCAAA+TGG + chr8_1:73385198-73385217 Msa1213250:intron 15.0%
!! ATTACTAATAGCATAAATTA+TGG - chr8_1:73385301-73385320 None:intergenic 15.0%
!! TCTATATATATATATATAGG+GGG - chr8_1:73385708-73385727 None:intergenic 15.0%
!! GTCTATATATATATATATAG+GGG - chr8_1:73385709-73385728 None:intergenic 15.0%
!! GTGTCTATATATATATATAT+AGG - chr8_1:73385711-73385730 None:intergenic 15.0%
!!! TTTTTTCTTTCTATTGTAAT+TGG + chr8_1:73384803-73384822 Msa1213250:intron 15.0%
!!! AAATGTATTTTAGATTTGTT+TGG + chr8_1:73385136-73385155 Msa1213250:intron 15.0%
!! TTCTCATTTATTTAATCAAA+TGG + chr8_1:73385198-73385217 Msa1213250:intron 15.0%
!! ATTACTAATAGCATAAATTA+TGG - chr8_1:73385301-73385320 None:intergenic 15.0%
!! TCTATATATATATATATAGG+GGG - chr8_1:73385708-73385727 None:intergenic 15.0%
!! GTCTATATATATATATATAG+GGG - chr8_1:73385709-73385728 None:intergenic 15.0%
!! GTGTCTATATATATATATAT+AGG - chr8_1:73385711-73385730 None:intergenic 15.0%
!! TAATTGGATTAATGTGAAAA+GGG + chr8_1:73384819-73384838 Msa1213250:intron 20.0%
!! CATTAAATGATATATAGTCT+CGG + chr8_1:73385034-73385053 Msa1213250:intron 20.0%
!!! ATAAAACTGACTTTTAAATG+AGG - chr8_1:73385084-73385103 None:intergenic 20.0%
!! AAAGTTTAATAGAGACAAAA+AGG + chr8_1:73385607-73385626 Msa1213250:intron 20.0%
!! TATGACTATATCAATGAAAT+TGG + chr8_1:73386054-73386073 Msa1213250:three_prime_UTR 20.0%
!! TATATCAATGAAATTGGTTA+TGG + chr8_1:73386060-73386079 Msa1213250:three_prime_UTR 20.0%
!!! TTCAAGTTCAAATTATTAAC+TGG + chr8_1:73386171-73386190 Msa1213250:three_prime_UTR 20.0%
!! TAATTGGATTAATGTGAAAA+GGG + chr8_1:73384819-73384838 Msa1213250:intron 20.0%
!! CATTAAATGATATATAGTCT+CGG + chr8_1:73385034-73385053 Msa1213250:intron 20.0%
!!! ATAAAACTGACTTTTAAATG+AGG - chr8_1:73385084-73385103 None:intergenic 20.0%
!! AAAGTTTAATAGAGACAAAA+AGG + chr8_1:73385607-73385626 Msa1213250:intron 20.0%
!! TATGACTATATCAATGAAAT+TGG + chr8_1:73386054-73386073 Msa1213250:three_prime_UTR 20.0%
!! TATATCAATGAAATTGGTTA+TGG + chr8_1:73386060-73386079 Msa1213250:three_prime_UTR 20.0%
!!! TTCAAGTTCAAATTATTAAC+TGG + chr8_1:73386171-73386190 Msa1213250:three_prime_UTR 20.0%
! GAATCAATTAATCAAAGACA+TGG + chr8_1:73384444-73384463 Msa1213250:exon 25.0%
! GTAATTGGATTAATGTGAAA+AGG + chr8_1:73384818-73384837 Msa1213250:intron 25.0%
! AATTGGATTAATGTGAAAAG+GGG + chr8_1:73384820-73384839 Msa1213250:intron 25.0%
!!! TGTTCTATTGATTCATTACT+AGG + chr8_1:73384863-73384882 Msa1213250:intron 25.0%
!! TGTTACTTGATATTGTGTTA+AGG + chr8_1:73385006-73385025 Msa1213250:intron 25.0%
! GTTACTTGATATTGTGTTAA+GGG + chr8_1:73385007-73385026 Msa1213250:intron 25.0%
!! TTGTTAACCTTAGTGTTTTT+AGG - chr8_1:73385491-73385510 None:intergenic 25.0%
!!! TGTTTTGGTAAATAACTAGT+AGG - chr8_1:73385520-73385539 None:intergenic 25.0%
!! ATAGAAAAACATGAGTGTTT+TGG - chr8_1:73385535-73385554 None:intergenic 25.0%
! TATATAGACACATATGCATA+TGG + chr8_1:73385720-73385739 Msa1213250:intron 25.0%
! TGAATATAGTATTGCTTGAA+GGG + chr8_1:73385776-73385795 Msa1213250:CDS 25.0%
!! AAAGAAAACGTTCTATTTTG+TGG + chr8_1:73385866-73385885 Msa1213250:CDS 25.0%
!!! TCATAAAAACTTTTGGCTTT+TGG + chr8_1:73386026-73386045 Msa1213250:CDS 25.0%
! GAATCAATTAATCAAAGACA+TGG + chr8_1:73384444-73384463 Msa1213250:exon 25.0%
! GTAATTGGATTAATGTGAAA+AGG + chr8_1:73384818-73384837 Msa1213250:intron 25.0%
! AATTGGATTAATGTGAAAAG+GGG + chr8_1:73384820-73384839 Msa1213250:intron 25.0%
!!! TGTTCTATTGATTCATTACT+AGG + chr8_1:73384863-73384882 Msa1213250:intron 25.0%
!! TGTTACTTGATATTGTGTTA+AGG + chr8_1:73385006-73385025 Msa1213250:intron 25.0%
! GTTACTTGATATTGTGTTAA+GGG + chr8_1:73385007-73385026 Msa1213250:intron 25.0%
!! TTGTTAACCTTAGTGTTTTT+AGG - chr8_1:73385491-73385510 None:intergenic 25.0%
!!! TGTTTTGGTAAATAACTAGT+AGG - chr8_1:73385520-73385539 None:intergenic 25.0%
!! ATAGAAAAACATGAGTGTTT+TGG - chr8_1:73385535-73385554 None:intergenic 25.0%
! TATATAGACACATATGCATA+TGG + chr8_1:73385720-73385739 Msa1213250:intron 25.0%
! TGAATATAGTATTGCTTGAA+GGG + chr8_1:73385776-73385795 Msa1213250:CDS 25.0%
!! AAAGAAAACGTTCTATTTTG+TGG + chr8_1:73385866-73385885 Msa1213250:CDS 25.0%
!!! TCATAAAAACTTTTGGCTTT+TGG + chr8_1:73386026-73386045 Msa1213250:CDS 25.0%
TGATGTGAAGTTTAATCTGA+AGG + chr8_1:73384895-73384914 Msa1213250:CDS 30.0%
TCTCGGTATAAGTTTATAAG+AGG + chr8_1:73385051-73385070 Msa1213250:intron 30.0%
TATAAGTTTATAAGAGGAGG+CGG + chr8_1:73385057-73385076 Msa1213250:intron 30.0%
!!! TAACCTTAGTGTTTTTAGGT+GGG - chr8_1:73385487-73385506 None:intergenic 30.0%
!!! TTAACCTTAGTGTTTTTAGG+TGG - chr8_1:73385488-73385507 None:intergenic 30.0%
!! CGTTTTAGTAAAGTACAAGT+AGG + chr8_1:73385630-73385649 Msa1213250:intron 30.0%
GTGAATATAGTATTGCTTGA+AGG + chr8_1:73385775-73385794 Msa1213250:CDS 30.0%
GAATATAGTATTGCTTGAAG+GGG + chr8_1:73385777-73385796 Msa1213250:CDS 30.0%
AGGAGGAAGAATAAAAAACT+TGG + chr8_1:73385979-73385998 Msa1213250:CDS 30.0%
AGGAAGAATAAAAAACTTGG+AGG + chr8_1:73385982-73386001 Msa1213250:CDS 30.0%
GGAAGAATAAAAAACTTGGA+GGG + chr8_1:73385983-73386002 Msa1213250:CDS 30.0%
! CCAAAAGTTTTTATGACGTT+TGG - chr8_1:73386022-73386041 None:intergenic 30.0%
! CCAAACGTCATAAAAACTTT+TGG + chr8_1:73386019-73386038 Msa1213250:CDS 30.0%
TGCCTAATACACATAAAGTA+AGG + chr8_1:73386097-73386116 Msa1213250:three_prime_UTR 30.0%
GCCTAATACACATAAAGTAA+GGG + chr8_1:73386098-73386117 Msa1213250:three_prime_UTR 30.0%
ACCCTTACTTTATGTGTATT+AGG - chr8_1:73386102-73386121 None:intergenic 30.0%
TGATGTGAAGTTTAATCTGA+AGG + chr8_1:73384895-73384914 Msa1213250:CDS 30.0%
TCTCGGTATAAGTTTATAAG+AGG + chr8_1:73385051-73385070 Msa1213250:intron 30.0%
TATAAGTTTATAAGAGGAGG+CGG + chr8_1:73385057-73385076 Msa1213250:intron 30.0%
!!! TAACCTTAGTGTTTTTAGGT+GGG - chr8_1:73385487-73385506 None:intergenic 30.0%
!!! TTAACCTTAGTGTTTTTAGG+TGG - chr8_1:73385488-73385507 None:intergenic 30.0%
!! CGTTTTAGTAAAGTACAAGT+AGG + chr8_1:73385630-73385649 Msa1213250:intron 30.0%
GTGAATATAGTATTGCTTGA+AGG + chr8_1:73385775-73385794 Msa1213250:CDS 30.0%
GAATATAGTATTGCTTGAAG+GGG + chr8_1:73385777-73385796 Msa1213250:CDS 30.0%
AGGAGGAAGAATAAAAAACT+TGG + chr8_1:73385979-73385998 Msa1213250:CDS 30.0%
AGGAAGAATAAAAAACTTGG+AGG + chr8_1:73385982-73386001 Msa1213250:CDS 30.0%
GGAAGAATAAAAAACTTGGA+GGG + chr8_1:73385983-73386002 Msa1213250:CDS 30.0%
! CCAAAAGTTTTTATGACGTT+TGG - chr8_1:73386022-73386041 None:intergenic 30.0%
! CCAAACGTCATAAAAACTTT+TGG + chr8_1:73386019-73386038 Msa1213250:CDS 30.0%
TGCCTAATACACATAAAGTA+AGG + chr8_1:73386097-73386116 Msa1213250:three_prime_UTR 30.0%
GCCTAATACACATAAAGTAA+GGG + chr8_1:73386098-73386117 Msa1213250:three_prime_UTR 30.0%
ACCCTTACTTTATGTGTATT+AGG - chr8_1:73386102-73386121 None:intergenic 30.0%
AAACTATGCAACTTGAAACG+TGG - chr8_1:73384475-73384494 None:intergenic 35.0%
!! AGAACTGAGTTTTCAAGCAA+CGG + chr8_1:73384568-73384587 Msa1213250:CDS 35.0%
! ACTCTGGAAGAGATAACAAA+GGG + chr8_1:73384599-73384618 Msa1213250:CDS 35.0%
TGAAAGTGGCAATAAAGTGA+TGG + chr8_1:73384624-73384643 Msa1213250:CDS 35.0%
CAATAAAGTGATGGTTGTTG+TGG + chr8_1:73384633-73384652 Msa1213250:CDS 35.0%
!! GATTCTAGCTTTGAAGCTAA+AGG + chr8_1:73384655-73384674 Msa1213250:CDS 35.0%
GAACAAGAACAACAGTATCT+TGG - chr8_1:73384717-73384736 None:intergenic 35.0%
!!! TTTTTTCTGACCTTCTCTGA+GGG - chr8_1:73384752-73384771 None:intergenic 35.0%
CACACATACTCTTCATATCT+AGG - chr8_1:73384934-73384953 None:intergenic 35.0%
ATGTGTGAAATGAAGAAACC+TGG + chr8_1:73384947-73384966 Msa1213250:CDS 35.0%
TGTGTGAAATGAAGAAACCT+GGG + chr8_1:73384948-73384967 Msa1213250:CDS 35.0%
CGGTATAAGTTTATAAGAGG+AGG + chr8_1:73385054-73385073 Msa1213250:intron 35.0%
! ACTTGCACCTTAGTGTTTTT+AGG + chr8_1:73385665-73385684 Msa1213250:intron 35.0%
CATATGCATATGGTAACATG+TGG + chr8_1:73385730-73385749 Msa1213250:intron 35.0%
ATGGTAACATGTGGAGTTTA+TGG + chr8_1:73385739-73385758 Msa1213250:intron 35.0%
TGGTAACATGTGGAGTTTAT+GGG + chr8_1:73385740-73385759 Msa1213250:intron 35.0%
ACAAGAAAGAGAACCAAAAG+TGG + chr8_1:73385907-73385926 Msa1213250:CDS 35.0%
CAAGAAAGAGAACCAAAAGT+GGG + chr8_1:73385908-73385927 Msa1213250:CDS 35.0%
AAGAAAGAGAACCAAAAGTG+GGG + chr8_1:73385909-73385928 Msa1213250:CDS 35.0%
AAATTGGTTATGGTGTCTGT+GGG + chr8_1:73386070-73386089 Msa1213250:three_prime_UTR 35.0%
! TTCCCCTTGCTTGAATATTT+TGG + chr8_1:73386209-73386228 Msa1213250:three_prime_UTR 35.0%
GACCAAAATATTCAAGCAAG+GGG - chr8_1:73386214-73386233 None:intergenic 35.0%
GGACCAAAATATTCAAGCAA+GGG - chr8_1:73386215-73386234 None:intergenic 35.0%
AGGACCAAAATATTCAAGCA+AGG - chr8_1:73386216-73386235 None:intergenic 35.0%
AAACTATGCAACTTGAAACG+TGG - chr8_1:73384475-73384494 None:intergenic 35.0%
!! AGAACTGAGTTTTCAAGCAA+CGG + chr8_1:73384568-73384587 Msa1213250:CDS 35.0%
! ACTCTGGAAGAGATAACAAA+GGG + chr8_1:73384599-73384618 Msa1213250:CDS 35.0%
TGAAAGTGGCAATAAAGTGA+TGG + chr8_1:73384624-73384643 Msa1213250:CDS 35.0%
CAATAAAGTGATGGTTGTTG+TGG + chr8_1:73384633-73384652 Msa1213250:CDS 35.0%
!! GATTCTAGCTTTGAAGCTAA+AGG + chr8_1:73384655-73384674 Msa1213250:CDS 35.0%
GAACAAGAACAACAGTATCT+TGG - chr8_1:73384717-73384736 None:intergenic 35.0%
!!! TTTTTTCTGACCTTCTCTGA+GGG - chr8_1:73384752-73384771 None:intergenic 35.0%
CACACATACTCTTCATATCT+AGG - chr8_1:73384934-73384953 None:intergenic 35.0%
ATGTGTGAAATGAAGAAACC+TGG + chr8_1:73384947-73384966 Msa1213250:CDS 35.0%
TGTGTGAAATGAAGAAACCT+GGG + chr8_1:73384948-73384967 Msa1213250:CDS 35.0%
CGGTATAAGTTTATAAGAGG+AGG + chr8_1:73385054-73385073 Msa1213250:intron 35.0%
! ACTTGCACCTTAGTGTTTTT+AGG + chr8_1:73385665-73385684 Msa1213250:intron 35.0%
CATATGCATATGGTAACATG+TGG + chr8_1:73385730-73385749 Msa1213250:intron 35.0%
ATGGTAACATGTGGAGTTTA+TGG + chr8_1:73385739-73385758 Msa1213250:intron 35.0%
TGGTAACATGTGGAGTTTAT+GGG + chr8_1:73385740-73385759 Msa1213250:intron 35.0%
ACAAGAAAGAGAACCAAAAG+TGG + chr8_1:73385907-73385926 Msa1213250:CDS 35.0%
CAAGAAAGAGAACCAAAAGT+GGG + chr8_1:73385908-73385927 Msa1213250:CDS 35.0%
AAGAAAGAGAACCAAAAGTG+GGG + chr8_1:73385909-73385928 Msa1213250:CDS 35.0%
AAATTGGTTATGGTGTCTGT+GGG + chr8_1:73386070-73386089 Msa1213250:three_prime_UTR 35.0%
! TTCCCCTTGCTTGAATATTT+TGG + chr8_1:73386209-73386228 Msa1213250:three_prime_UTR 35.0%
GACCAAAATATTCAAGCAAG+GGG - chr8_1:73386214-73386233 None:intergenic 35.0%
GGACCAAAATATTCAAGCAA+GGG - chr8_1:73386215-73386234 None:intergenic 35.0%
AGGACCAAAATATTCAAGCA+AGG - chr8_1:73386216-73386235 None:intergenic 35.0%
! AAGTTGCATAGTTTGTGCGT+TGG + chr8_1:73384481-73384500 Msa1213250:CDS 40.0%
! GCATGTTCTGCATCTTCTTT+TGG + chr8_1:73384529-73384548 Msa1213250:CDS 40.0%
! GACTCTGGAAGAGATAACAA+AGG + chr8_1:73384598-73384617 Msa1213250:CDS 40.0%
! CTCTGGAAGAGATAACAAAG+GGG + chr8_1:73384600-73384619 Msa1213250:CDS 40.0%
GATAACAAAGGGGATGAAAG+TGG + chr8_1:73384610-73384629 Msa1213250:CDS 40.0%
!! AAGCTAAAGGTGCTCTTGAT+TGG + chr8_1:73384668-73384687 Msa1213250:CDS 40.0%
!! AGCTAAAGGTGCTCTTGATT+GGG + chr8_1:73384669-73384688 Msa1213250:CDS 40.0%
! TTTTTCTGACCTTCTCTGAG+GGG - chr8_1:73384751-73384770 None:intergenic 40.0%
!!! CTTTTTTCTGACCTTCTCTG+AGG - chr8_1:73384753-73384772 None:intergenic 40.0%
GTGTGAAATGAAGAAACCTG+GGG + chr8_1:73384949-73384968 Msa1213250:CDS 40.0%
CTTGTTTGAAAACTTACCCC+AGG - chr8_1:73384968-73384987 None:intergenic 40.0%
TCAAATGGTTCGACTCATCA+CGG + chr8_1:73385213-73385232 Msa1213250:intron 40.0%
!!! TGCACCTTAGTGTTTTTAGG+TGG + chr8_1:73385668-73385687 Msa1213250:intron 40.0%
!!! GCACCTTAGTGTTTTTAGGT+GGG + chr8_1:73385669-73385688 Msa1213250:intron 40.0%
ATGTGGAGTTTATGGGTTTC+AGG + chr8_1:73385747-73385766 Msa1213250:intron 40.0%
TATAGTATTGCTTGAAGGGG+AGG + chr8_1:73385780-73385799 Msa1213250:CDS 40.0%
!! GCAACAAAGGGTGTCTTTAT+TGG + chr8_1:73385834-73385853 Msa1213250:CDS 40.0%
!! ACAAAGGGTGTCTTTATTGG+TGG + chr8_1:73385837-73385856 Msa1213250:CDS 40.0%
! CTATTTTGTGGTGCTTGATG+AGG + chr8_1:73385878-73385897 Msa1213250:CDS 40.0%
GAAATTGGTTATGGTGTCTG+TGG + chr8_1:73386069-73386088 Msa1213250:three_prime_UTR 40.0%
AGAAAGCTCGTTCAAAGTGA+AGG - chr8_1:73386236-73386255 None:intergenic 40.0%
! AAGTTGCATAGTTTGTGCGT+TGG + chr8_1:73384481-73384500 Msa1213250:CDS 40.0%
! GCATGTTCTGCATCTTCTTT+TGG + chr8_1:73384529-73384548 Msa1213250:CDS 40.0%
! GACTCTGGAAGAGATAACAA+AGG + chr8_1:73384598-73384617 Msa1213250:CDS 40.0%
! CTCTGGAAGAGATAACAAAG+GGG + chr8_1:73384600-73384619 Msa1213250:CDS 40.0%
GATAACAAAGGGGATGAAAG+TGG + chr8_1:73384610-73384629 Msa1213250:CDS 40.0%
!! AAGCTAAAGGTGCTCTTGAT+TGG + chr8_1:73384668-73384687 Msa1213250:CDS 40.0%
!! AGCTAAAGGTGCTCTTGATT+GGG + chr8_1:73384669-73384688 Msa1213250:CDS 40.0%
! TTTTTCTGACCTTCTCTGAG+GGG - chr8_1:73384751-73384770 None:intergenic 40.0%
!!! CTTTTTTCTGACCTTCTCTG+AGG - chr8_1:73384753-73384772 None:intergenic 40.0%
GTGTGAAATGAAGAAACCTG+GGG + chr8_1:73384949-73384968 Msa1213250:CDS 40.0%
CTTGTTTGAAAACTTACCCC+AGG - chr8_1:73384968-73384987 None:intergenic 40.0%
TCAAATGGTTCGACTCATCA+CGG + chr8_1:73385213-73385232 Msa1213250:intron 40.0%
!!! TGCACCTTAGTGTTTTTAGG+TGG + chr8_1:73385668-73385687 Msa1213250:intron 40.0%
!!! GCACCTTAGTGTTTTTAGGT+GGG + chr8_1:73385669-73385688 Msa1213250:intron 40.0%
ATGTGGAGTTTATGGGTTTC+AGG + chr8_1:73385747-73385766 Msa1213250:intron 40.0%
TATAGTATTGCTTGAAGGGG+AGG + chr8_1:73385780-73385799 Msa1213250:CDS 40.0%
!! GCAACAAAGGGTGTCTTTAT+TGG + chr8_1:73385834-73385853 Msa1213250:CDS 40.0%
!! ACAAAGGGTGTCTTTATTGG+TGG + chr8_1:73385837-73385856 Msa1213250:CDS 40.0%
! CTATTTTGTGGTGCTTGATG+AGG + chr8_1:73385878-73385897 Msa1213250:CDS 40.0%
GAAATTGGTTATGGTGTCTG+TGG + chr8_1:73386069-73386088 Msa1213250:three_prime_UTR 40.0%
AGAAAGCTCGTTCAAAGTGA+AGG - chr8_1:73386236-73386255 None:intergenic 40.0%
GAGAGTGGTGACTCAATAGT+AGG - chr8_1:73385167-73385186 None:intergenic 45.0%
AGAAGTCTTTGTCTCGAGAG+TGG - chr8_1:73385182-73385201 None:intergenic 45.0%
GCACCCACCTAAAAACACTA+AGG + chr8_1:73385481-73385500 Msa1213250:intron 45.0%
GCACCCACCTAAAAACACTA+AGG - chr8_1:73385481-73385500 None:intergenic 45.0%
ATTGCTTGAAGGGGAGGAAA+AGG + chr8_1:73385786-73385805 Msa1213250:CDS 45.0%
TTGCTTGAAGGGGAGGAAAA+GGG + chr8_1:73385787-73385806 Msa1213250:CDS 45.0%
TGCAAGAGGCAAAGCAACAA+AGG + chr8_1:73385821-73385840 Msa1213250:CDS 45.0%
GCAAGAGGCAAAGCAACAAA+GGG + chr8_1:73385822-73385841 Msa1213250:CDS 45.0%
!! AAGGGTGTCTTTATTGGTGG+TGG + chr8_1:73385840-73385859 Msa1213250:CDS 45.0%
! AGGGTGTCTTTATTGGTGGT+GGG + chr8_1:73385841-73385860 Msa1213250:CDS 45.0%
! ATACTCAGCAACCCCACTTT+TGG - chr8_1:73385923-73385942 None:intergenic 45.0%
CTCACTGCAATGGTCATACA+AGG - chr8_1:73385961-73385980 None:intergenic 45.0%
CTTGTATGACCATTGCAGTG+AGG + chr8_1:73385959-73385978 Msa1213250:CDS 45.0%
ATTCTTCCTCCTCACTGCAA+TGG - chr8_1:73385971-73385990 None:intergenic 45.0%
GAGAGTGGTGACTCAATAGT+AGG - chr8_1:73385167-73385186 None:intergenic 45.0%
AGAAGTCTTTGTCTCGAGAG+TGG - chr8_1:73385182-73385201 None:intergenic 45.0%
GCACCCACCTAAAAACACTA+AGG + chr8_1:73385481-73385500 Msa1213250:intron 45.0%
GCACCCACCTAAAAACACTA+AGG - chr8_1:73385481-73385500 None:intergenic 45.0%
ATTGCTTGAAGGGGAGGAAA+AGG + chr8_1:73385786-73385805 Msa1213250:CDS 45.0%
TTGCTTGAAGGGGAGGAAAA+GGG + chr8_1:73385787-73385806 Msa1213250:CDS 45.0%
TGCAAGAGGCAAAGCAACAA+AGG + chr8_1:73385821-73385840 Msa1213250:CDS 45.0%
GCAAGAGGCAAAGCAACAAA+GGG + chr8_1:73385822-73385841 Msa1213250:CDS 45.0%
!! AAGGGTGTCTTTATTGGTGG+TGG + chr8_1:73385840-73385859 Msa1213250:CDS 45.0%
! AGGGTGTCTTTATTGGTGGT+GGG + chr8_1:73385841-73385860 Msa1213250:CDS 45.0%
! ATACTCAGCAACCCCACTTT+TGG - chr8_1:73385923-73385942 None:intergenic 45.0%
CTCACTGCAATGGTCATACA+AGG - chr8_1:73385961-73385980 None:intergenic 45.0%
CTTGTATGACCATTGCAGTG+AGG + chr8_1:73385959-73385978 Msa1213250:CDS 45.0%
ATTCTTCCTCCTCACTGCAA+TGG - chr8_1:73385971-73385990 None:intergenic 45.0%
!!! TAAAATACATTTTAAAATTT+GGG - chr8_1:73385128-73385147 None:intergenic 5.0%
!!! TAATTATTTTAATTAATCTA+TGG - chr8_1:73385348-73385367 None:intergenic 5.0%
!! ATATATATATATATATATAG+GGG + chr8_1:73385448-73385467 Msa1213250:intron 5.0%
!!! TAAAATACATTTTAAAATTT+GGG - chr8_1:73385128-73385147 None:intergenic 5.0%
!!! TAATTATTTTAATTAATCTA+TGG - chr8_1:73385348-73385367 None:intergenic 5.0%
!! ATATATATATATATATATAG+GGG + chr8_1:73385448-73385467 Msa1213250:intron 5.0%
AGCAACGGAGTTCAAGACTC+TGG + chr8_1:73384583-73384602 Msa1213250:CDS 50.0%
TGTGGTGCTTGATGAGGAAG+TGG + chr8_1:73385884-73385903 Msa1213250:CDS 50.0%
GTATGACCATTGCAGTGAGG+AGG + chr8_1:73385962-73385981 Msa1213250:CDS 50.0%
AGCAACGGAGTTCAAGACTC+TGG + chr8_1:73384583-73384602 Msa1213250:CDS 50.0%
TGTGGTGCTTGATGAGGAAG+TGG + chr8_1:73385884-73385903 Msa1213250:CDS 50.0%
GTATGACCATTGCAGTGAGG+AGG + chr8_1:73385962-73385981 Msa1213250:CDS 50.0%
GCAGAACATGCTCGAACACG+AGG - chr8_1:73384520-73384539 None:intergenic 55.0%
GTAGCTAGACCCCTCAGAGA+AGG + chr8_1:73384739-73384758 Msa1213250:CDS 55.0%
! GGGTGCTGCAATAGTGCAAG+AGG + chr8_1:73385807-73385826 Msa1213250:CDS 55.0%
GCAGAACATGCTCGAACACG+AGG - chr8_1:73384520-73384539 None:intergenic 55.0%
GTAGCTAGACCCCTCAGAGA+AGG + chr8_1:73384739-73384758 Msa1213250:CDS 55.0%
! GGGTGCTGCAATAGTGCAAG+AGG + chr8_1:73385807-73385826 Msa1213250:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 73384428 73386299 73384428 ID=Msa1213250;Name=Msa1213250
chr8_1 mRNA 73384428 73386299 73384428 ID=Msa1213250-mRNA-1;Parent=Msa1213250;Name=Msa1213250-mRNA-1;_AED=0.03;_eAED=0.03;_QI=35|1|1|1|1|1|3|246|222
chr8_1 exon 73384428 73384760 73384428 ID=Msa1213250-mRNA-1:exon:24890;Parent=Msa1213250-mRNA-1
chr8_1 exon 73384885 73384970 73384885 ID=Msa1213250-mRNA-1:exon:24891;Parent=Msa1213250-mRNA-1
chr8_1 exon 73385769 73386299 73385769 ID=Msa1213250-mRNA-1:exon:24892;Parent=Msa1213250-mRNA-1
chr8_1 five_prime_UTR 73384428 73384462 73384428 ID=Msa1213250-mRNA-1:five_prime_utr;Parent=Msa1213250-mRNA-1
chr8_1 CDS 73384463 73384760 73384463 ID=Msa1213250-mRNA-1:cds;Parent=Msa1213250-mRNA-1
chr8_1 CDS 73384885 73384970 73384885 ID=Msa1213250-mRNA-1:cds;Parent=Msa1213250-mRNA-1
chr8_1 CDS 73385769 73386053 73385769 ID=Msa1213250-mRNA-1:cds;Parent=Msa1213250-mRNA-1
chr8_1 three_prime_UTR 73386054 73386299 73386054 ID=Msa1213250-mRNA-1:three_prime_utr;Parent=Msa1213250-mRNA-1
Gene Sequence

>Msa1213250

ATGGTTAGACCACGTTTCAAGTTGCATAGTTTGTGCGTTGGTCATGCTAGAACACCTCGTGTTCGAGCATGTTCTGCATCTTCTTTTGGATCAAAATTTGCAAACAGAACTGAGTTTTCAAGCAACGGAGTTCAAGACTCTGGAAGAGATAACAAAGGGGATGAAAGTGGCAATAAAGTGATGGTTGTTGTGGATTCTAGCTTTGAAGCTAAAGGTGCTCTTGATTGGGCACTCTCACACACTATTCAGAGCCAAGATACTGTTGTTCTTGTTCATGTAGCTAGACCCCTCAGAGAAGGCGACGAGTCTGATGTGAAGTTTAATCTGAAGGCATATCAACTTCTCCTAGATATGAAGAGTATGTGTGAAATGAAGAAACCTGGGGTGCTAGTGAATATAGTATTGCTTGAAGGGGAGGAAAAGGGTGCTGCAATAGTGCAAGAGGCAAAGCAACAAAGGGTGTCTTTATTGGTGGTGGGACAAAGAAAACGTTCTATTTTGTGGTGCTTGATGAGGAAGTGGACAAGAAAGAGAACCAAAAGTGGGGTTGCTGAGTATTGTATACAGAACTCACCTTGTATGACCATTGCAGTGAGGAGGAAGAATAAAAAACTTGGAGGGTATTTGATTACTACCAAACGTCATAAAAACTTTTGGCTTTTGGCTTAG
Protein sequence

>Msa1213250

MVRPRFKLHSLCVGHARTPRVRACSASSFGSKFANRTEFSSNGVQDSGRDNKGDESGNKVMVVVDSSFEAKGALDWALSHTIQSQDTVVLVHVARPLREGDESDVKFNLKAYQLLLDMKSMCEMKKPGVLVNIVLLEGEEKGAAIVQEAKQQRVSLLVVGQRKRSILWCLMRKWTRKRTKSGVAEYCIQNSPCMTIAVRRKNKKLGGYLITTKRHKNFWLLA*