AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1233060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1233060 MtrunA17_Chr4g0000171 63.333 180 66 0 1 180 153 332 2.04e-78 237
Msa1233060 MtrunA17_Chr8g0339861 66.667 171 57 0 1 171 155 325 2.33e-76 236
Msa1233060 MtrunA17_Chr4g0000161 62.209 172 65 0 1 172 153 324 2.15e-73 224
Msa1233060 MtrunA17_Chr4g0013471 61.988 171 65 0 2 172 154 324 2.81e-71 221
Msa1233060 MtrunA17_Chr8g0381551 59.770 174 68 2 1 173 157 329 6.80e-69 213
Msa1233060 MtrunA17_Chr8g0381571 59.302 172 68 2 1 171 157 327 7.58e-66 209
Msa1233060 MtrunA17_Chr8g0381541 59.302 172 68 2 1 171 157 327 1.01e-65 209
Msa1233060 MtrunA17_Chr1g0161671 58.192 177 73 1 1 177 143 318 1.22e-60 191
Msa1233060 MtrunA17_Chr2g0293421 41.520 171 98 2 1 171 159 327 9.05e-38 135
Msa1233060 MtrunA17_Chr2g0293411 42.197 173 96 3 1 171 155 325 1.22e-37 135
Msa1233060 MtrunA17_Chr1g0161651 57.143 126 53 1 1 126 195 319 7.27e-37 130
Msa1233060 MtrunA17_Chr5g0400421 44.444 171 92 3 2 171 160 328 1.40e-30 115
Msa1233060 MtrunA17_Chr5g0400411 42.529 174 92 3 2 171 158 327 2.22e-24 99.0
Msa1233060 MtrunA17_Chr1g0161691 54.878 82 36 1 1 82 56 136 2.31e-24 92.8
Msa1233060 MtrunA17_Chr1g0153371 31.765 170 112 2 3 172 145 310 2.40e-19 84.7
Msa1233060 MtrunA17_Chr3g0089381 29.655 145 98 2 33 177 3 143 1.31e-18 79.7
Msa1233060 MtrunA17_Chr4g0044851 30.058 173 115 2 3 175 147 313 1.37e-18 82.4
Msa1233060 MtrunA17_Chr8g0388921 35.821 134 85 1 31 164 177 309 1.86e-18 82.0
Msa1233060 MtrunA17_Chr3g0089361 29.530 149 100 2 29 177 181 324 1.29e-16 76.6
Msa1233060 MtrunA17_Chr6g0455461 30.899 178 107 5 6 175 138 307 4.43e-16 75.1
Msa1233060 MtrunA17_Chr5g0436991 37.273 110 62 3 64 173 55 157 4.53e-16 71.6
Msa1233060 MtrunA17_Chr3g0078411 34.646 127 76 3 49 175 188 307 7.38e-16 74.7
Msa1233060 MtrunA17_Chr3g0089351 28.859 149 101 2 29 177 99 242 1.50e-15 73.6
Msa1233060 MtrunA17_Chr7g0219961 30.058 173 106 5 6 171 138 302 1.93e-15 73.2
Msa1233060 MtrunA17_Chr3g0089291 28.873 142 96 2 29 170 99 235 5.73e-15 70.5
Msa1233060 MtrunA17_Chr3g0113321 31.707 164 102 6 11 171 147 303 1.56e-13 67.8
Msa1233060 MtrunA17_Chr4g0044841 27.841 176 121 2 3 178 148 317 2.10e-13 67.4
Msa1233060 MtrunA17_Chr8g0369791 29.286 140 89 3 29 168 170 299 7.36e-13 65.9
Msa1233060 MtrunA17_Chr5g0408181 29.630 135 85 3 29 163 168 292 1.39e-12 65.1
Msa1233060 MtrunA17_Chr8g0372411 29.375 160 97 7 17 169 165 315 2.62e-12 64.3
Msa1233060 MtrunA17_Chr5g0443731 24.540 163 122 1 2 164 142 303 4.77e-12 63.5
Msa1233060 MtrunA17_Chr8g0380471 32.061 131 88 1 34 164 179 308 1.24e-11 62.4
Msa1233060 MtrunA17_Chr5g0443401 29.730 148 96 5 24 169 174 315 1.30e-11 62.4
Msa1233060 MtrunA17_Chr5g0397971 28.966 145 94 4 29 169 166 305 6.10e-11 60.5
Msa1233060 MtrunA17_Chr8g0341951 25.974 154 107 3 22 175 170 316 8.46e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1233060 AT1G05160.2 39.181 171 102 2 2 172 160 328 5.10e-34 125
Msa1233060 AT1G05160.1 39.181 171 102 2 2 172 160 328 5.10e-34 125
Msa1233060 AT2G32440.2 39.181 171 102 2 1 171 158 326 4.00e-33 123
Msa1233060 AT2G32440.1 39.181 171 102 2 1 171 158 326 4.00e-33 123
Msa1233060 AT5G36110.2 33.333 165 95 4 14 169 154 312 4.87e-20 86.7
Msa1233060 AT5G36110.1 37.143 140 81 3 31 169 179 312 1.28e-19 85.9
Msa1233060 AT5G36140.1 34.286 140 85 3 31 169 175 308 9.84e-18 79.3
Msa1233060 AT3G19270.1 30.189 159 102 4 3 160 144 294 3.98e-14 70.1
Msa1233060 AT3G19270.2 30.189 159 102 4 3 160 144 294 4.86e-14 69.7
Msa1233060 AT2G29090.4 27.869 183 124 5 3 184 159 334 6.04e-14 68.9
Msa1233060 AT4G19230.2 29.193 161 98 6 6 164 149 295 5.81e-13 66.6
Msa1233060 AT4G19230.1 29.091 165 101 6 6 168 149 299 6.07e-13 66.6
Msa1233060 AT5G45340.2 27.108 166 109 5 3 168 146 299 2.17e-12 65.1
Msa1233060 AT5G45340.1 27.108 166 109 5 3 168 146 299 2.98e-12 64.7
Msa1233060 AT4G36380.2 24.490 147 107 3 24 169 204 347 1.04e-11 62.8
Msa1233060 AT4G36380.1 24.490 147 107 3 24 169 204 347 1.27e-11 62.8
Msa1233060 AT2G29090.3 27.976 168 113 5 3 169 64 224 2.82e-11 61.6
Msa1233060 AT2G29090.2 27.976 168 113 5 3 169 159 319 3.28e-11 61.6
Msa1233060 AT2G29090.1 27.976 168 113 5 3 169 159 319 3.28e-11 61.6
Msa1233060 AT1G73340.1 29.078 141 89 4 31 169 185 316 7.18e-11 60.5
Msa1233060 AT5G38970.2 24.359 156 107 3 24 177 83 229 8.43e-11 60.1
Msa1233060 AT5G38970.4 24.359 156 107 3 24 177 83 229 8.43e-11 60.1

Find 52 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTAGCTTAGTGATCGATTT+TGG 0.175863 8_2:-9684575 None:intergenic
TGTACATAACTAATGTATTT+TGG 0.219074 8_2:-9684668 None:intergenic
AGCAAAACCAGGTGCCTTTA+TGG 0.221809 8_2:-9684892 None:intergenic
AAATGGAAATACATTGGATT+TGG 0.256548 8_2:+9683981 Msa1233060:five_prime_UTR
GACATGGGATGGCCTATTAT+TGG 0.274318 8_2:+9684121 Msa1233060:five_prime_UTR
AGATGAAAAGTGTTTGAAAA+AGG 0.282931 8_2:-9684155 None:intergenic
AAACATTCTTCATCTCATTA+AGG 0.297114 8_2:-9684761 None:intergenic
CCGGTCATCGTCAATAAATT+TGG 0.306182 8_2:+9684825 Msa1233060:CDS
TGGAGATTCATTTAACGTTA+TGG 0.314929 8_2:+9684845 Msa1233060:CDS
AATCGATCACTAAGCTAGTA+TGG 0.334069 8_2:+9684579 Msa1233060:five_prime_UTR
ATCTCATTAAGGAACTCAAC+AGG 0.376829 8_2:-9684750 None:intergenic
CTGATAGCGCTATTGCTTAG+TGG 0.378215 8_2:+9685198 Msa1233060:CDS
GCAAAACCAGGTGCCTTTAT+GGG 0.400526 8_2:-9684891 None:intergenic
ACATGGGATGGCCTATTATT+GGG 0.401969 8_2:+9684122 Msa1233060:five_prime_UTR
ATGTCATTGACAAATTCATC+AGG 0.411182 8_2:-9684184 None:intergenic
GGCCACATGTTCCCAATAAT+AGG 0.413146 8_2:-9684133 None:intergenic
TCATTTAACGTTATGGCTGC+TGG 0.422801 8_2:+9684852 Msa1233060:CDS
TTTGGAAATAAACGACGAAA+AGG 0.428658 8_2:+9685140 Msa1233060:CDS
ATGTACAAGACACACTTGTA+TGG 0.428727 8_2:+9684473 Msa1233060:five_prime_UTR
CCCATGTCACCAGGAGGTAG+TGG 0.466764 8_2:-9684106 None:intergenic
CCAAATTTATTGACGATGAC+CGG 0.477081 8_2:-9684825 None:intergenic
AATACCTATGTTCACCACTT+AGG 0.479455 8_2:-9684614 None:intergenic
AAATAGTAGGTTATTTACCT+TGG 0.481345 8_2:-9685295 None:intergenic
TCCACTACCTCCTGGTGACA+TGG 0.483202 8_2:+9684105 Msa1233060:five_prime_UTR
TACCTCCTGGTGACATGGGA+TGG 0.489770 8_2:+9684110 Msa1233060:five_prime_UTR
TACAATCTATTGTTGATGAA+AGG 0.498853 8_2:+9685061 Msa1233060:CDS
TGCTTTCAACAAAGCACTCA+AGG 0.500116 8_2:+9684911 Msa1233060:CDS
AATTTAGGCACGTAAGAAGT+TGG 0.501299 8_2:+9685029 Msa1233060:intron
GACAGATGATGTGAACTTTG+AGG 0.520859 8_2:+9684544 Msa1233060:five_prime_UTR
TTGGAAATAAACGACGAAAA+GGG 0.523297 8_2:+9685141 Msa1233060:CDS
GCAGAAGTGACTATGATGCT+TGG 0.539245 8_2:-9684500 None:intergenic
TTGGCTTTCTTCAAACAAAG+TGG 0.553244 8_2:-9685276 None:intergenic
CAAGTGTGTCTTGTACATAA+CGG 0.560883 8_2:-9684468 None:intergenic
ATAGGCCATCCCATGTCACC+AGG 0.570615 8_2:-9684115 None:intergenic
TTTATGCCCATAAAGGCACC+TGG 0.571604 8_2:+9684885 Msa1233060:CDS
TTCTGCTGCTATGTGCAAGA+AGG 0.579434 8_2:+9684517 Msa1233060:five_prime_UTR
GATACCTAAGTGGTGAACAT+AGG 0.580999 8_2:+9684610 Msa1233060:five_prime_UTR
CCACTACCTCCTGGTGACAT+GGG 0.587904 8_2:+9684106 Msa1233060:five_prime_UTR
GGCCTATTATTGGGAACATG+TGG 0.591942 8_2:+9684131 Msa1233060:five_prime_UTR
TTGTGAGGAATTTGAATGTG+TGG 0.606895 8_2:+9684050 Msa1233060:five_prime_UTR
CTAGTCACTTCTCCTATCGT+TGG 0.613545 8_2:+9684645 Msa1233060:five_prime_UTR
GATGATGTGAACTTTGAGGA+AGG 0.613821 8_2:+9684548 Msa1233060:five_prime_UTR
AAAGGACATCCACTACCTCC+TGG 0.616462 8_2:+9684097 Msa1233060:five_prime_UTR
GCTTTGTTGAAAGCAAAACC+AGG 0.621354 8_2:-9684903 None:intergenic
ATTGTAGCATCCTAGCAAAG+TGG 0.639328 8_2:-9684014 None:intergenic
TCTCATTAAGGAACTCAACA+GGG 0.659553 8_2:-9684749 None:intergenic
CATTAGCAACTACTATGATG+TGG 0.659770 8_2:+9685229 Msa1233060:CDS
TGTCATTGACAAATTCATCA+GGG 0.660537 8_2:-9684183 None:intergenic
ACTTTGTTTGAAGAAAGCCA+AGG 0.670445 8_2:+9685278 Msa1233060:CDS
TATGTACATACAACGCATTG+AGG 0.680590 8_2:+9684683 Msa1233060:exon
GGACTAGCTAGATACCTAAG+TGG 0.682431 8_2:+9684600 Msa1233060:five_prime_UTR
GGCCATCCCATGTCACCAGG+AGG 0.688276 8_2:-9684112 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTTCTATAAAAGAAAAAT+AGG + chr8_2:9683950-9683969 Msa1233060:five_prime_UTR 15.0%
!! AAGATTAAAGAAAAAGAAAA+AGG - chr8_2:9684247-9684266 None:intergenic 15.0%
!!! AATCTTTTTTCTATAATAGT+TGG + chr8_2:9684261-9684280 Msa1233060:intron 15.0%
!! TCTCAATAATAAAAAATAGT+AGG - chr8_2:9685311-9685330 None:intergenic 15.0%
!! AAGTTCTATAAAAGAAAAAT+AGG + chr8_2:9683950-9683969 Msa1233060:five_prime_UTR 15.0%
!! AAGATTAAAGAAAAAGAAAA+AGG - chr8_2:9684247-9684266 None:intergenic 15.0%
!!! AATCTTTTTTCTATAATAGT+TGG + chr8_2:9684261-9684280 Msa1233060:intron 15.0%
!! TCTCAATAATAAAAAATAGT+AGG - chr8_2:9685311-9685330 None:intergenic 15.0%
!!! TTTTGTTTCTCTTTTGTAAA+TGG - chr8_2:9683932-9683951 None:intergenic 20.0%
!! AAAAATAGGAGATACAAAAA+TGG + chr8_2:9683964-9683983 Msa1233060:five_prime_UTR 20.0%
!! ATACAAAAATGGAAATACAT+TGG + chr8_2:9683975-9683994 Msa1233060:five_prime_UTR 20.0%
!! AAAGAAAAAGGAAAAAAGTA+GGG - chr8_2:9684235-9684254 None:intergenic 20.0%
!! AAAAGAAAAAGGAAAAAAGT+AGG - chr8_2:9684236-9684255 None:intergenic 20.0%
!!! TGTACATAACTAATGTATTT+TGG - chr8_2:9684671-9684690 None:intergenic 20.0%
!!! AATGTGAAAGATGATTTTAA+AGG - chr8_2:9684787-9684806 None:intergenic 20.0%
!!! AATCATCTTTCACATTTTTT+TGG + chr8_2:9684791-9684810 Msa1233060:CDS 20.0%
!!! ATCATCTTTCACATTTTTTT+GGG + chr8_2:9684792-9684811 Msa1233060:CDS 20.0%
!! AAGAAGAATGAGAAAATAAA+AGG - chr8_2:9684959-9684978 None:intergenic 20.0%
!!! AGATCTTTTAGATATTCTTT+TGG + chr8_2:9685122-9685141 Msa1233060:CDS 20.0%
!!! TTTTGTTTCTCTTTTGTAAA+TGG - chr8_2:9683932-9683951 None:intergenic 20.0%
!! AAAAATAGGAGATACAAAAA+TGG + chr8_2:9683964-9683983 Msa1233060:five_prime_UTR 20.0%
!! ATACAAAAATGGAAATACAT+TGG + chr8_2:9683975-9683994 Msa1233060:five_prime_UTR 20.0%
!! AAAGAAAAAGGAAAAAAGTA+GGG - chr8_2:9684235-9684254 None:intergenic 20.0%
!! AAAAGAAAAAGGAAAAAAGT+AGG - chr8_2:9684236-9684255 None:intergenic 20.0%
!!! TGTACATAACTAATGTATTT+TGG - chr8_2:9684671-9684690 None:intergenic 20.0%
!!! AATGTGAAAGATGATTTTAA+AGG - chr8_2:9684787-9684806 None:intergenic 20.0%
!!! AATCATCTTTCACATTTTTT+TGG + chr8_2:9684791-9684810 Msa1233060:CDS 20.0%
!!! ATCATCTTTCACATTTTTTT+GGG + chr8_2:9684792-9684811 Msa1233060:CDS 20.0%
!! AAGAAGAATGAGAAAATAAA+AGG - chr8_2:9684959-9684978 None:intergenic 20.0%
!!! AGATCTTTTAGATATTCTTT+TGG + chr8_2:9685122-9685141 Msa1233060:CDS 20.0%
! AAATGGAAATACATTGGATT+TGG + chr8_2:9683981-9684000 Msa1233060:five_prime_UTR 25.0%
!! GATTTTAAACTGAGAAAGAA+AGG + chr8_2:9684079-9684098 Msa1233060:five_prime_UTR 25.0%
!!! GATGAAAAGTGTTTGAAAAA+GGG - chr8_2:9684157-9684176 None:intergenic 25.0%
!!! AGATGAAAAGTGTTTGAAAA+AGG - chr8_2:9684158-9684177 None:intergenic 25.0%
!!! TTTTTCAAACACTTTTCATC+TGG + chr8_2:9684157-9684176 Msa1233060:five_prime_UTR 25.0%
! CATCAACAACAAGTTAATAT+TGG - chr8_2:9684431-9684450 None:intergenic 25.0%
! AAACATTCTTCATCTCATTA+AGG - chr8_2:9684764-9684783 None:intergenic 25.0%
! TCACTTTGTATACCTAATTT+AGG + chr8_2:9685014-9685033 Msa1233060:intron 25.0%
!! TACAATCTATTGTTGATGAA+AGG + chr8_2:9685061-9685080 Msa1233060:CDS 25.0%
! AAATAGTAGGTTATTTACCT+TGG - chr8_2:9685298-9685317 None:intergenic 25.0%
! AAATGGAAATACATTGGATT+TGG + chr8_2:9683981-9684000 Msa1233060:five_prime_UTR 25.0%
!! GATTTTAAACTGAGAAAGAA+AGG + chr8_2:9684079-9684098 Msa1233060:five_prime_UTR 25.0%
!!! GATGAAAAGTGTTTGAAAAA+GGG - chr8_2:9684157-9684176 None:intergenic 25.0%
!!! AGATGAAAAGTGTTTGAAAA+AGG - chr8_2:9684158-9684177 None:intergenic 25.0%
!!! TTTTTCAAACACTTTTCATC+TGG + chr8_2:9684157-9684176 Msa1233060:five_prime_UTR 25.0%
! CATCAACAACAAGTTAATAT+TGG - chr8_2:9684431-9684450 None:intergenic 25.0%
! AAACATTCTTCATCTCATTA+AGG - chr8_2:9684764-9684783 None:intergenic 25.0%
! TCACTTTGTATACCTAATTT+AGG + chr8_2:9685014-9685033 Msa1233060:intron 25.0%
!! TACAATCTATTGTTGATGAA+AGG + chr8_2:9685061-9685080 Msa1233060:CDS 25.0%
! AAATAGTAGGTTATTTACCT+TGG - chr8_2:9685298-9685317 None:intergenic 25.0%
! ATTTTGCTGACAAAATTGTG+AGG + chr8_2:9684035-9684054 Msa1233060:five_prime_UTR 30.0%
TGTCATTGACAAATTCATCA+GGG - chr8_2:9684186-9684205 None:intergenic 30.0%
ATGTCATTGACAAATTCATC+AGG - chr8_2:9684187-9684206 None:intergenic 30.0%
ACGAAGAAAGTAAATAGCAA+AGG - chr8_2:9684294-9684313 None:intergenic 30.0%
TGTTGTTGATGTTTCAGATA+TGG + chr8_2:9684440-9684459 Msa1233060:intron 30.0%
TGGAGATTCATTTAACGTTA+TGG + chr8_2:9684845-9684864 Msa1233060:CDS 30.0%
! ATTCTCTTTTATGCCCATAA+AGG + chr8_2:9684878-9684897 Msa1233060:CDS 30.0%
TTTGGAAATAAACGACGAAA+AGG + chr8_2:9685140-9685159 Msa1233060:CDS 30.0%
TTGGAAATAAACGACGAAAA+GGG + chr8_2:9685141-9685160 Msa1233060:CDS 30.0%
! ATTTTGCTGACAAAATTGTG+AGG + chr8_2:9684035-9684054 Msa1233060:five_prime_UTR 30.0%
TGTCATTGACAAATTCATCA+GGG - chr8_2:9684186-9684205 None:intergenic 30.0%
ATGTCATTGACAAATTCATC+AGG - chr8_2:9684187-9684206 None:intergenic 30.0%
ACGAAGAAAGTAAATAGCAA+AGG - chr8_2:9684294-9684313 None:intergenic 30.0%
TGTTGTTGATGTTTCAGATA+TGG + chr8_2:9684440-9684459 Msa1233060:intron 30.0%
TGGAGATTCATTTAACGTTA+TGG + chr8_2:9684845-9684864 Msa1233060:CDS 30.0%
! ATTCTCTTTTATGCCCATAA+AGG + chr8_2:9684878-9684897 Msa1233060:CDS 30.0%
TTTGGAAATAAACGACGAAA+AGG + chr8_2:9685140-9685159 Msa1233060:CDS 30.0%
TTGGAAATAAACGACGAAAA+GGG + chr8_2:9685141-9685160 Msa1233060:CDS 30.0%
!!! ATTTTTGTTGCCACTTTGCT+AGG + chr8_2:9684004-9684023 Msa1233060:five_prime_UTR 35.0%
TTGTGAGGAATTTGAATGTG+TGG + chr8_2:9684050-9684069 Msa1233060:five_prime_UTR 35.0%
CAAGTGTGTCTTGTACATAA+CGG - chr8_2:9684471-9684490 None:intergenic 35.0%
ATGTACAAGACACACTTGTA+TGG + chr8_2:9684473-9684492 Msa1233060:five_prime_UTR 35.0%
!! ACTAGCTTAGTGATCGATTT+TGG - chr8_2:9684578-9684597 None:intergenic 35.0%
AATCGATCACTAAGCTAGTA+TGG + chr8_2:9684579-9684598 Msa1233060:five_prime_UTR 35.0%
AATACCTATGTTCACCACTT+AGG - chr8_2:9684617-9684636 None:intergenic 35.0%
!!! AATGTATTTTGGCCAACGAT+AGG - chr8_2:9684660-9684679 None:intergenic 35.0%
TATGTACATACAACGCATTG+AGG + chr8_2:9684683-9684702 Msa1233060:exon 35.0%
TCTCATTAAGGAACTCAACA+GGG - chr8_2:9684752-9684771 None:intergenic 35.0%
ATCTCATTAAGGAACTCAAC+AGG - chr8_2:9684753-9684772 None:intergenic 35.0%
CCAAATTTATTGACGATGAC+CGG - chr8_2:9684828-9684847 None:intergenic 35.0%
ACTTCTTACGTGCCTAAATT+AGG - chr8_2:9685029-9685048 None:intergenic 35.0%
AATTTAGGCACGTAAGAAGT+TGG + chr8_2:9685029-9685048 Msa1233060:intron 35.0%
CGAAAAGGGTGAAAAATTAG+AGG + chr8_2:9685155-9685174 Msa1233060:CDS 35.0%
CATTAGCAACTACTATGATG+TGG + chr8_2:9685229-9685248 Msa1233060:CDS 35.0%
! TTGGCTTTCTTCAAACAAAG+TGG - chr8_2:9685279-9685298 None:intergenic 35.0%
! ACTTTGTTTGAAGAAAGCCA+AGG + chr8_2:9685278-9685297 Msa1233060:CDS 35.0%
!!! ATTTTTGTTGCCACTTTGCT+AGG + chr8_2:9684004-9684023 Msa1233060:five_prime_UTR 35.0%
TTGTGAGGAATTTGAATGTG+TGG + chr8_2:9684050-9684069 Msa1233060:five_prime_UTR 35.0%
CAAGTGTGTCTTGTACATAA+CGG - chr8_2:9684471-9684490 None:intergenic 35.0%
ATGTACAAGACACACTTGTA+TGG + chr8_2:9684473-9684492 Msa1233060:five_prime_UTR 35.0%
!! ACTAGCTTAGTGATCGATTT+TGG - chr8_2:9684578-9684597 None:intergenic 35.0%
AATCGATCACTAAGCTAGTA+TGG + chr8_2:9684579-9684598 Msa1233060:five_prime_UTR 35.0%
AATACCTATGTTCACCACTT+AGG - chr8_2:9684617-9684636 None:intergenic 35.0%
!!! AATGTATTTTGGCCAACGAT+AGG - chr8_2:9684660-9684679 None:intergenic 35.0%
TATGTACATACAACGCATTG+AGG + chr8_2:9684683-9684702 Msa1233060:exon 35.0%
TCTCATTAAGGAACTCAACA+GGG - chr8_2:9684752-9684771 None:intergenic 35.0%
ATCTCATTAAGGAACTCAAC+AGG - chr8_2:9684753-9684772 None:intergenic 35.0%
CCAAATTTATTGACGATGAC+CGG - chr8_2:9684828-9684847 None:intergenic 35.0%
ACTTCTTACGTGCCTAAATT+AGG - chr8_2:9685029-9685048 None:intergenic 35.0%
AATTTAGGCACGTAAGAAGT+TGG + chr8_2:9685029-9685048 Msa1233060:intron 35.0%
CGAAAAGGGTGAAAAATTAG+AGG + chr8_2:9685155-9685174 Msa1233060:CDS 35.0%
CATTAGCAACTACTATGATG+TGG + chr8_2:9685229-9685248 Msa1233060:CDS 35.0%
! TTGGCTTTCTTCAAACAAAG+TGG - chr8_2:9685279-9685298 None:intergenic 35.0%
! ACTTTGTTTGAAGAAAGCCA+AGG + chr8_2:9685278-9685297 Msa1233060:CDS 35.0%
ATTGTAGCATCCTAGCAAAG+TGG - chr8_2:9684017-9684036 None:intergenic 40.0%
ACATGGGATGGCCTATTATT+GGG + chr8_2:9684122-9684141 Msa1233060:five_prime_UTR 40.0%
GACAGATGATGTGAACTTTG+AGG + chr8_2:9684544-9684563 Msa1233060:five_prime_UTR 40.0%
! GATGATGTGAACTTTGAGGA+AGG + chr8_2:9684548-9684567 Msa1233060:five_prime_UTR 40.0%
GATACCTAAGTGGTGAACAT+AGG + chr8_2:9684610-9684629 Msa1233060:five_prime_UTR 40.0%
!!! TTTTTTGGGATCTTGCGATC+CGG + chr8_2:9684806-9684825 Msa1233060:CDS 40.0%
CCGGTCATCGTCAATAAATT+TGG + chr8_2:9684825-9684844 Msa1233060:CDS 40.0%
TCATTTAACGTTATGGCTGC+TGG + chr8_2:9684852-9684871 Msa1233060:CDS 40.0%
! GCTTTGTTGAAAGCAAAACC+AGG - chr8_2:9684906-9684925 None:intergenic 40.0%
! TGCTTTCAACAAAGCACTCA+AGG + chr8_2:9684911-9684930 Msa1233060:CDS 40.0%
! TTCAACAAAGCACTCAAGGT+TGG + chr8_2:9684915-9684934 Msa1233060:intron 40.0%
!! AGGAAAGCGCTGCTAAAAAA+TGG + chr8_2:9685081-9685100 Msa1233060:CDS 40.0%
!! GGAAAGCGCTGCTAAAAAAT+GGG + chr8_2:9685082-9685101 Msa1233060:CDS 40.0%
ATTGTAGCATCCTAGCAAAG+TGG - chr8_2:9684017-9684036 None:intergenic 40.0%
ACATGGGATGGCCTATTATT+GGG + chr8_2:9684122-9684141 Msa1233060:five_prime_UTR 40.0%
GACAGATGATGTGAACTTTG+AGG + chr8_2:9684544-9684563 Msa1233060:five_prime_UTR 40.0%
! GATGATGTGAACTTTGAGGA+AGG + chr8_2:9684548-9684567 Msa1233060:five_prime_UTR 40.0%
GATACCTAAGTGGTGAACAT+AGG + chr8_2:9684610-9684629 Msa1233060:five_prime_UTR 40.0%
!!! TTTTTTGGGATCTTGCGATC+CGG + chr8_2:9684806-9684825 Msa1233060:CDS 40.0%
CCGGTCATCGTCAATAAATT+TGG + chr8_2:9684825-9684844 Msa1233060:CDS 40.0%
TCATTTAACGTTATGGCTGC+TGG + chr8_2:9684852-9684871 Msa1233060:CDS 40.0%
! GCTTTGTTGAAAGCAAAACC+AGG - chr8_2:9684906-9684925 None:intergenic 40.0%
! TGCTTTCAACAAAGCACTCA+AGG + chr8_2:9684911-9684930 Msa1233060:CDS 40.0%
! TTCAACAAAGCACTCAAGGT+TGG + chr8_2:9684915-9684934 Msa1233060:intron 40.0%
!! AGGAAAGCGCTGCTAAAAAA+TGG + chr8_2:9685081-9685100 Msa1233060:CDS 40.0%
!! GGAAAGCGCTGCTAAAAAAT+GGG + chr8_2:9685082-9685101 Msa1233060:CDS 40.0%
GACATGGGATGGCCTATTAT+TGG + chr8_2:9684121-9684140 Msa1233060:five_prime_UTR 45.0%
GGCCTATTATTGGGAACATG+TGG + chr8_2:9684131-9684150 Msa1233060:five_prime_UTR 45.0%
GGCCACATGTTCCCAATAAT+AGG - chr8_2:9684136-9684155 None:intergenic 45.0%
GCAGAAGTGACTATGATGCT+TGG - chr8_2:9684503-9684522 None:intergenic 45.0%
TTCTGCTGCTATGTGCAAGA+AGG + chr8_2:9684517-9684536 Msa1233060:five_prime_UTR 45.0%
!! GGACTAGCTAGATACCTAAG+TGG + chr8_2:9684600-9684619 Msa1233060:five_prime_UTR 45.0%
CTAGTCACTTCTCCTATCGT+TGG + chr8_2:9684645-9684664 Msa1233060:five_prime_UTR 45.0%
!! TTTATGCCCATAAAGGCACC+TGG + chr8_2:9684885-9684904 Msa1233060:CDS 45.0%
GCAAAACCAGGTGCCTTTAT+GGG - chr8_2:9684894-9684913 None:intergenic 45.0%
AGCAAAACCAGGTGCCTTTA+TGG - chr8_2:9684895-9684914 None:intergenic 45.0%
! CTGATAGCGCTATTGCTTAG+TGG + chr8_2:9685198-9685217 Msa1233060:CDS 45.0%
GACATGGGATGGCCTATTAT+TGG + chr8_2:9684121-9684140 Msa1233060:five_prime_UTR 45.0%
GGCCTATTATTGGGAACATG+TGG + chr8_2:9684131-9684150 Msa1233060:five_prime_UTR 45.0%
GGCCACATGTTCCCAATAAT+AGG - chr8_2:9684136-9684155 None:intergenic 45.0%
GCAGAAGTGACTATGATGCT+TGG - chr8_2:9684503-9684522 None:intergenic 45.0%
TTCTGCTGCTATGTGCAAGA+AGG + chr8_2:9684517-9684536 Msa1233060:five_prime_UTR 45.0%
!! GGACTAGCTAGATACCTAAG+TGG + chr8_2:9684600-9684619 Msa1233060:five_prime_UTR 45.0%
CTAGTCACTTCTCCTATCGT+TGG + chr8_2:9684645-9684664 Msa1233060:five_prime_UTR 45.0%
!! TTTATGCCCATAAAGGCACC+TGG + chr8_2:9684885-9684904 Msa1233060:CDS 45.0%
GCAAAACCAGGTGCCTTTAT+GGG - chr8_2:9684894-9684913 None:intergenic 45.0%
AGCAAAACCAGGTGCCTTTA+TGG - chr8_2:9684895-9684914 None:intergenic 45.0%
! CTGATAGCGCTATTGCTTAG+TGG + chr8_2:9685198-9685217 Msa1233060:CDS 45.0%
AAAGGACATCCACTACCTCC+TGG + chr8_2:9684097-9684116 Msa1233060:five_prime_UTR 50.0%
AAAGGACATCCACTACCTCC+TGG + chr8_2:9684097-9684116 Msa1233060:five_prime_UTR 50.0%
TCCACTACCTCCTGGTGACA+TGG + chr8_2:9684105-9684124 Msa1233060:five_prime_UTR 55.0%
CCACTACCTCCTGGTGACAT+GGG + chr8_2:9684106-9684125 Msa1233060:five_prime_UTR 55.0%
TACCTCCTGGTGACATGGGA+TGG + chr8_2:9684110-9684129 Msa1233060:five_prime_UTR 55.0%
ATAGGCCATCCCATGTCACC+AGG - chr8_2:9684118-9684137 None:intergenic 55.0%
TCCACTACCTCCTGGTGACA+TGG + chr8_2:9684105-9684124 Msa1233060:five_prime_UTR 55.0%
CCACTACCTCCTGGTGACAT+GGG + chr8_2:9684106-9684125 Msa1233060:five_prime_UTR 55.0%
TACCTCCTGGTGACATGGGA+TGG + chr8_2:9684110-9684129 Msa1233060:five_prime_UTR 55.0%
ATAGGCCATCCCATGTCACC+AGG - chr8_2:9684118-9684137 None:intergenic 55.0%
CCCATGTCACCAGGAGGTAG+TGG - chr8_2:9684109-9684128 None:intergenic 60.0%
CCCATGTCACCAGGAGGTAG+TGG - chr8_2:9684109-9684128 None:intergenic 60.0%
GGCCATCCCATGTCACCAGG+AGG - chr8_2:9684115-9684134 None:intergenic 65.0%
GGCCATCCCATGTCACCAGG+AGG - chr8_2:9684115-9684134 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 9683922 9685341 9683922 ID=Msa1233060;Name=Msa1233060
chr8_2 mRNA 9683922 9685341 9683922 ID=Msa1233060-mRNA-1;Parent=Msa1233060;Name=Msa1233060-mRNA-1;_AED=0.08;_eAED=0.08;_QI=521|1|0.66|1|1|0.66|3|0|184
chr8_2 exon 9683922 9684215 9683922 ID=Msa1233060-mRNA-1:exon:3862;Parent=Msa1233060-mRNA-1
chr8_2 exon 9684457 9684932 9684457 ID=Msa1233060-mRNA-1:exon:3863;Parent=Msa1233060-mRNA-1
chr8_2 exon 9685036 9685341 9685036 ID=Msa1233060-mRNA-1:exon:3864;Parent=Msa1233060-mRNA-1
chr8_2 five_prime_UTR 9683922 9684215 9683922 ID=Msa1233060-mRNA-1:five_prime_utr;Parent=Msa1233060-mRNA-1
chr8_2 five_prime_UTR 9684457 9684683 9684457 ID=Msa1233060-mRNA-1:five_prime_utr;Parent=Msa1233060-mRNA-1
chr8_2 CDS 9684684 9684932 9684684 ID=Msa1233060-mRNA-1:cds;Parent=Msa1233060-mRNA-1
chr8_2 CDS 9685036 9685341 9685036 ID=Msa1233060-mRNA-1:cds;Parent=Msa1233060-mRNA-1
Gene Sequence

>Msa1233060

ATGTACATACAACGCATTGAGGATATTATAATCGATAAGCTCGAAGAATTGTCTAGCATGAAACACCCTGTTGAGTTCCTTAATGAGATGAAGAATGTTTCCTTTAAAATCATCTTTCACATTTTTTTGGGATCTTGCGATCCGGTCATCGTCAATAAATTTGGAGATTCATTTAACGTTATGGCTGCTGGTATATTCTCTTTTATGCCCATAAAGGCACCTGGTTTTGCTTTCAACAAAGCACTCAAGGCACGTAAGAAGTTGGTCAAAATATTACAATCTATTGTTGATGAAAGGAAAGCGCTGCTAAAAAATGGGCAAATCGAAGAAAAGAAAGATCTTTTAGATATTCTTTTGGAAATAAACGACGAAAAGGGTGAAAAATTAGAGGTTGATGACATTATTGACTTTCTGATAGCGCTATTGCTTAGTGGTCATGAAACATTAGCAACTACTATGATGTGGTTAATTACATATCTTACAGAACATCCACTTTGTTTGAAGAAAGCCAAGGTAAATAACCTACTATTTTTTATTATTGAGAATTTTTTATGA
Protein sequence

>Msa1233060

MYIQRIEDIIIDKLEELSSMKHPVEFLNEMKNVSFKIIFHIFLGSCDPVIVNKFGDSFNVMAAGIFSFMPIKAPGFAFNKALKARKKLVKILQSIVDERKALLKNGQIEEKKDLLDILLEINDEKGEKLEVDDIIDFLIALLLSGHETLATTMMWLITYLTEHPLCLKKAKVNNLLFFIIENFL*