AlfalfaGEDB | Msa1237120

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1237120	XP_039687880.1	90.291	103	10	0	1	103	660	762	6.70e-58	202

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1237120	A0A392MXL2	66.990	103	34	0	1	103	133	235	1.12e-43	153

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0813470	Msa1237120	0.819476	1.138448e-52	-8.615850e-47
Msa1237120	Msa1242860	0.817456	3.267303e-52	-8.615850e-47
Msa0872030	Msa1237120	0.810151	1.329563e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1237120	MtrunA17_Chr3g0101871	64.368	87	31	0	1	87	1014	1100	1.62e-34	124
Msa1237120	MtrunA17_Chr1g0206501	36.275	102	64	1	3	103	130	231	2.28e-15	70.1
Msa1237120	MtrunA17_Chr1g0173681	43.077	65	37	0	1	65	271	335	1.39e-12	62.0
Msa1237120	MtrunA17_Chr7g0269661	34.951	103	67	0	1	103	39	141	3.21e-12	60.1

Find 19 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAATTGTTTGCTTCTCTTAC+AGG	0.168521	8_2:+16373745	Msa1237120:CDS
AAAATTTGTTCTTGAACAAT+AGG	0.292476	8_2:-16373718	None:intergenic
TGGATAGGCCTAATCATGTT+TGG	0.353529	8_2:+16373375	Msa1237120:CDS
GGTTATGTTGCTGATTTCTT+CGG	0.388583	8_2:+16373661	Msa1237120:CDS
GGATAGGCCTAATCATGTTT+GGG	0.424172	8_2:+16373376	Msa1237120:CDS
AAATCAGCAACATAACCATT+TGG	0.456921	8_2:-16373655	None:intergenic
AACACTTGCAATCTAACAAA+AGG	0.468910	8_2:+16373602	Msa1237120:CDS
TCATGGAAGTTCCTCTCTGA+TGG	0.479207	8_2:+16373404	Msa1237120:CDS
TATTGTAGCTTTAATTCTAA+CGG	0.497576	8_2:+16373540	Msa1237120:intron
ATCATGTTTGGGAAAACTCA+TGG	0.501729	8_2:+16373387	Msa1237120:CDS
AGCTTTAATTCTAACGGATG+AGG	0.502130	8_2:+16373546	Msa1237120:intron
ATGGAATTCTTTATGATCAA+AGG	0.507330	8_2:+16373423	Msa1237120:CDS
AAATGCATGCCGTATCCAAA+TGG	0.527092	8_2:+16373640	Msa1237120:CDS
AGGAGGCTCACACGAAATCC+AGG	0.529108	8_2:+16373443	Msa1237120:CDS
ACAGATTAATACATGATGAA+AGG	0.569383	8_2:+16373686	Msa1237120:CDS
TACTGTCTCAATCAATGGAT+AGG	0.594802	8_2:+16373360	None:intergenic
GCAACATAACCATTTGGATA+CGG	0.603860	8_2:-16373649	None:intergenic
GAATTCTTTATGATCAAAGG+AGG	0.629297	8_2:+16373426	Msa1237120:CDS
CATAAAGAATTCCATCAGAG+AGG	0.668846	8_2:-16373415	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGCTACAATATAATAACAT+TGG	-	chr8_2:16373531-16373550	None:intergenic	20.0%
!!!	TATTGTAGCTTTAATTCTAA+CGG	+	chr8_2:16373540-16373559	Msa1237120:intron	20.0%
!!!	AAAATTTGTTCTTGAACAAT+AGG	-	chr8_2:16373721-16373740	None:intergenic	20.0%
!	ATGGAATTCTTTATGATCAA+AGG	+	chr8_2:16373423-16373442	Msa1237120:CDS	25.0%
!	ACAGATTAATACATGATGAA+AGG	+	chr8_2:16373686-16373705	Msa1237120:CDS	25.0%
	GAATTCTTTATGATCAAAGG+AGG	+	chr8_2:16373426-16373445	Msa1237120:CDS	30.0%
	ATTTGTTTCAACATTGTACC+TGG	-	chr8_2:16373464-16373483	None:intergenic	30.0%
	AACACTTGCAATCTAACAAA+AGG	+	chr8_2:16373602-16373621	Msa1237120:CDS	30.0%
	AAATCAGCAACATAACCATT+TGG	-	chr8_2:16373658-16373677	None:intergenic	30.0%
!	GAGTTTTCCCAAACATGATT+AGG	-	chr8_2:16373386-16373405	None:intergenic	35.0%
	ATCATGTTTGGGAAAACTCA+TGG	+	chr8_2:16373387-16373406	Msa1237120:CDS	35.0%
	CATAAAGAATTCCATCAGAG+AGG	-	chr8_2:16373418-16373437	None:intergenic	35.0%
!	AGCTTTAATTCTAACGGATG+AGG	+	chr8_2:16373546-16373565	Msa1237120:intron	35.0%
	GCAACATAACCATTTGGATA+CGG	-	chr8_2:16373652-16373671	None:intergenic	35.0%
!!	GGTTATGTTGCTGATTTCTT+CGG	+	chr8_2:16373661-16373680	Msa1237120:CDS	35.0%
	TGGATAGGCCTAATCATGTT+TGG	+	chr8_2:16373375-16373394	Msa1237120:CDS	40.0%
	GGATAGGCCTAATCATGTTT+GGG	+	chr8_2:16373376-16373395	Msa1237120:CDS	40.0%
	AAATGCATGCCGTATCCAAA+TGG	+	chr8_2:16373640-16373659	Msa1237120:CDS	40.0%
	TCATGGAAGTTCCTCTCTGA+TGG	+	chr8_2:16373404-16373423	Msa1237120:CDS	45.0%
	AGGAGGCTCACACGAAATCC+AGG	+	chr8_2:16373443-16373462	Msa1237120:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	16373374	16373765	16373374	ID=Msa1237120;Name=Msa1237120
chr8_2	mRNA	16373374	16373765	16373374	ID=Msa1237120-mRNA-1;Parent=Msa1237120;Name=Msa1237120-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0\|0\|0\|1\|1\|1\|2\|0\|103
chr8_2	exon	16373374	16373464	16373374	ID=Msa1237120-mRNA-1:exon:6124;Parent=Msa1237120-mRNA-1
chr8_2	exon	16373548	16373765	16373548	ID=Msa1237120-mRNA-1:exon:6125;Parent=Msa1237120-mRNA-1
chr8_2	CDS	16373374	16373464	16373374	ID=Msa1237120-mRNA-1:cds;Parent=Msa1237120-mRNA-1
chr8_2	CDS	16373548	16373765	16373548	ID=Msa1237120-mRNA-1:cds;Parent=Msa1237120-mRNA-1

Gene Sequence	>Msa1237120 ATGGATAGGCCTAATCATGTTTGGGAAAACTCATGGAAGTTCCTCTCTGATGGAATTCTTTATGATCAAAGGAGGCTCACACGAAATCCAGCTTTAATTCTAACGGATGAGGAATTGCGAAATTTAACGCTGATTGAAATTGAGAAACACTTGCAATCTAACAAAAGGAGCTTGAAAGAGTTCAAATGCATGCCGTATCCAAATGGTTATGTTGCTGATTTCTTCGGAAACAGATTAATACATGATGAAAGGAATTATGATCCTATTGTTCAAGAACAAATTTTTCAACAATTGTTTGCTTCTCTTACA
Protein sequence	>Msa1237120 MDRPNHVWENSWKFLSDGILYDQRRLTRNPALILTDEELRNLTLIEIEKHLQSNKRSLKEFKCMPYPNGYVADFFGNRLIHDERNYDPIVQEQIFQQLFASLT

M. sativa cultivar zhongmu-4 / Msa1237120