AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1239740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1239740 MtrunA17_Chr8g0351501 100.000 106 0 0 1 106 1 106 2.35e-76 220
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1239740 AT5G18800.2 85.849 106 15 0 1 106 1 106 2.90e-65 192
Msa1239740 AT5G18800.1 85.849 106 15 0 1 106 1 106 2.90e-65 192
Msa1239740 AT3G06310.1 79.048 105 22 0 2 106 4 108 1.31e-60 181
Msa1239740 AT3G06310.3 79.048 105 22 0 2 106 4 108 1.31e-60 181
Msa1239740 AT3G06310.2 77.419 62 14 0 2 63 4 65 8.48e-32 107

Find 60 sgRNAs with CRISPR-Local

Find 291 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACAAAAGATCAAATGAATT+TGG 0.156685 8_2:-21667536 None:intergenic
CCATCTCCTTTGTGCACTTT+TGG 0.157227 8_2:-21667348 None:intergenic
AAGAGTTATTGTGAGTATTA+AGG 0.202243 8_2:+21663149 Msa1239740:five_prime_UTR
AATAATATCACAGGGCAATT+TGG 0.235688 8_2:+21667491 Msa1239740:three_prime_UTR
AAGATGTCATTCGGAGAATT+TGG 0.243844 8_2:+21663766 Msa1239740:CDS
TGACTACCTGCTACACATTC+AGG 0.274086 8_2:+21663259 Msa1239740:five_prime_UTR
ACTAACAAAATACACAATTA+AGG 0.276169 8_2:-21663002 None:intergenic
AGAGTTATTGTGAGTATTAA+GGG 0.292469 8_2:+21663150 Msa1239740:five_prime_UTR
GAAGATTGATGGTGAAGATT+AGG 0.293815 8_2:-21663446 None:intergenic
GTTGGTAGTCTAAATGCTTC+TGG 0.304274 8_2:+21667592 Msa1239740:three_prime_UTR
AAGATTGATGGTGAAGATTA+GGG 0.339205 8_2:-21663445 None:intergenic
TAAGGGATTATTTAAGCGTT+AGG 0.343544 8_2:+21663167 Msa1239740:five_prime_UTR
CAAATGCTTCCTGCTCTTTG+CGG 0.352587 8_2:-21667420 None:intergenic
ATCAAATGAATTTGGGAAAC+AGG 0.360204 8_2:-21667528 None:intergenic
TGCTCTAAACTAAGACTGTT+TGG 0.367685 8_2:+21667914 Msa1239740:three_prime_UTR
CCCAACATCAGCGGTGTTGA+TGG 0.386535 8_2:+21663721 Msa1239740:CDS
ACAAAAGATCAAATGAATTT+GGG 0.389383 8_2:-21667535 None:intergenic
TCATGTGTTTGTTCACTTTG+TGG 0.408772 8_2:+21667869 Msa1239740:three_prime_UTR
AGGTCATAATCGTTGTAGTT+TGG 0.415899 8_2:-21667764 None:intergenic
TCCTGAAAAGTGCCTTGATA+AGG 0.418124 8_2:+21663820 Msa1239740:CDS
AAGGGATTATTTAAGCGTTA+GGG 0.427602 8_2:+21663168 Msa1239740:five_prime_UTR
GATGTTACTCGCTGTGTCCT+TGG 0.430847 8_2:+21663848 Msa1239740:CDS
ATAAGTCGAATTCATTTGTA+TGG 0.438882 8_2:-21667396 None:intergenic
TACAACGATTATGACCTTGT+TGG 0.439184 8_2:+21667770 Msa1239740:three_prime_UTR
ATAATAAGTAAGTCTATTGA+AGG 0.440218 8_2:-21663074 None:intergenic
GACTAACAAAGTGAACAATC+AGG 0.440774 8_2:+21663199 Msa1239740:five_prime_UTR
CCATCAACACCGCTGATGTT+GGG 0.462363 8_2:-21663721 None:intergenic
CATTGGGTTAAGATGTCATT+CGG 0.471473 8_2:+21663757 Msa1239740:CDS
GCCATCAACACCGCTGATGT+TGG 0.471695 8_2:-21663722 None:intergenic
ATTGTTGAAAATAATATCAC+AGG 0.482137 8_2:+21667482 Msa1239740:three_prime_UTR
ACACCGCTGATGTTGGGATC+GGG 0.486192 8_2:-21663715 None:intergenic
CGACTTATGCCGCAAAGAGC+AGG 0.487600 8_2:+21667411 Msa1239740:CDS
CCTGAAAAGTGCCTTGATAA+GGG 0.493225 8_2:+21663821 Msa1239740:CDS
TTAAATACAAAGTTATTTGG+TGG 0.496328 8_2:-21663315 None:intergenic
TGGCATCTTCGAAGCACATT+GGG 0.505873 8_2:+21663741 Msa1239740:CDS
TTGGTCCCTGAATGTGTAGC+AGG 0.510311 8_2:-21663265 None:intergenic
TCGAGTGCTGTAGATGCTGC+TGG 0.511264 8_2:+21663689 Msa1239740:CDS
GACTACCTGCTACACATTCA+GGG 0.518471 8_2:+21663260 Msa1239740:five_prime_UTR
GGTGTGGGAAGGAAGATTGA+TGG 0.533826 8_2:-21663457 None:intergenic
CCAAAAGTGCACAAAGGAGA+TGG 0.538195 8_2:+21667348 Msa1239740:CDS
TAACATCACGTCCCTTATCA+AGG 0.539105 8_2:-21663832 None:intergenic
AACACCGCTGATGTTGGGAT+CGG 0.539717 8_2:-21663716 None:intergenic
TAATGGGGCCGTAGTCACTT+TGG 0.546902 8_2:-21663284 None:intergenic
TCTGCGATAACATCTTGTGT+TGG 0.550933 8_2:+21667829 Msa1239740:three_prime_UTR
AAGAAGAAACTCACAGTCCA+AGG 0.560779 8_2:-21663865 None:intergenic
ATGGCATCTTCGAAGCACAT+TGG 0.565008 8_2:+21663740 Msa1239740:CDS
ATTGAATTGCAGTGTGGTGT+GGG 0.565127 8_2:-21663472 None:intergenic
TTCAGGGACCAAAGTGACTA+CGG 0.567206 8_2:+21663276 Msa1239740:five_prime_UTR
TAGACTACCAACATAGTCAC+TGG 0.579924 8_2:-21667581 None:intergenic
AATTGAATTGCAGTGTGGTG+TGG 0.596145 8_2:-21663473 None:intergenic
TGTGTTTGTTCACTTTGTGG+AGG 0.603174 8_2:+21667872 Msa1239740:three_prime_UTR
ACTAACAAAGTGAACAATCA+GGG 0.603688 8_2:+21663200 Msa1239740:five_prime_UTR
AATTGCAGTGTGGTGTGGGA+AGG 0.605305 8_2:-21663468 None:intergenic
ATCTTTACCAGTGACTATGT+TGG 0.630873 8_2:+21667574 Msa1239740:three_prime_UTR
TTGTTGAAAATAATATCACA+GGG 0.662122 8_2:+21667483 Msa1239740:three_prime_UTR
AAACCCGATCCCAACATCAG+CGG 0.696688 8_2:+21663712 Msa1239740:CDS
CACTTTGTGGAGGAACAATG+TGG 0.705871 8_2:+21667882 Msa1239740:three_prime_UTR
TCTACACCAAAAGTGCACAA+AGG 0.729329 8_2:+21667342 Msa1239740:CDS
AAGGAGATGGATGATTACGT+CGG 0.730179 8_2:+21667361 Msa1239740:CDS
ACTTGAATTGAATTGCAGTG+TGG 0.737957 8_2:-21663478 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTAAATACAAAGTTATT+TGG - chr8_2:21663321-21663340 None:intergenic 10.0%
!!! CTGTTTTTATTTTAAAAATT+TGG + chr8_2:21664730-21664749 Msa1239740:intron 10.0%
!! GAAAATTTAATCTTTATTAA+CGG + chr8_2:21664969-21664988 Msa1239740:intron 10.0%
!!! TGATTTGTTTTTATTTAATA+TGG - chr8_2:21665959-21665978 None:intergenic 10.0%
!! CAGTAAAAAAAAAATTAATA+AGG - chr8_2:21666446-21666465 None:intergenic 10.0%
!!! CTTATTAATTTTTTTTTTAC+TGG + chr8_2:21666444-21666463 Msa1239740:intron 10.0%
!! TAAATATAATTTCATCATAA+TGG + chr8_2:21666564-21666583 Msa1239740:intron 10.0%
!!! AACGAAATATTTTAATATCA+AGG - chr8_2:21663037-21663056 None:intergenic 15.0%
!!! ACAATAACTCTTTTTATATA+AGG - chr8_2:21663141-21663160 None:intergenic 15.0%
!! AATGAAATTGAAATTGAAAA+TGG - chr8_2:21663515-21663534 None:intergenic 15.0%
!! AAAGACATAACATAAATTAA+GGG - chr8_2:21664255-21664274 None:intergenic 15.0%
!! TTAAAATAAAAACAGAAGAT+CGG - chr8_2:21664725-21664744 None:intergenic 15.0%
!! AGCATTTGAAAATATTATAT+TGG + chr8_2:21664811-21664830 Msa1239740:intron 15.0%
!! CGATAATAATTACAAAATAT+TGG - chr8_2:21665509-21665528 None:intergenic 15.0%
!! AAATTTAATATATAGGAAGA+AGG - chr8_2:21665841-21665860 None:intergenic 15.0%
!! TAAATCAAGAAATATTTACA+AGG + chr8_2:21666086-21666105 Msa1239740:intron 15.0%
!!! TATAAAGATACAGTTTTTTA+TGG + chr8_2:21666597-21666616 Msa1239740:intron 15.0%
!!! TACAATTTAGTTTTCTATAT+AGG - chr8_2:21666926-21666945 None:intergenic 15.0%
!! ATAATAAGTAAGTCTATTGA+AGG - chr8_2:21663077-21663096 None:intergenic 20.0%
!!! TTAGAATTTTGATACATTGA+AGG + chr8_2:21663229-21663248 Msa1239740:five_prime_UTR 20.0%
!! TTAAATACAAAGTTATTTGG+TGG - chr8_2:21663318-21663337 None:intergenic 20.0%
!! AATTGAAAATTTGGGAAAAT+GGG - chr8_2:21663546-21663565 None:intergenic 20.0%
!! AAATTGAAAATTTGGGAAAA+TGG - chr8_2:21663547-21663566 None:intergenic 20.0%
!!! AAAATTGTTATGTGATTTTC+TGG - chr8_2:21663621-21663640 None:intergenic 20.0%
!!! AAATCACATAACAATTTTCT+CGG + chr8_2:21663623-21663642 Msa1239740:intron 20.0%
!!! TTTTGTTGATTTTGTTTATG+TGG + chr8_2:21663914-21663933 Msa1239740:intron 20.0%
!!! TTTCTCAAAACTAGTTTTAT+TGG + chr8_2:21664007-21664026 Msa1239740:intron 20.0%
!!! TTCTCAAAACTAGTTTTATT+GGG + chr8_2:21664008-21664027 Msa1239740:intron 20.0%
!!! TTGCATAAACTATTTTTCAT+AGG + chr8_2:21664203-21664222 Msa1239740:intron 20.0%
!! CAAAGACATAACATAAATTA+AGG - chr8_2:21664256-21664275 None:intergenic 20.0%
!! TTATGTTATGTCTTTGAATT+TGG + chr8_2:21664260-21664279 Msa1239740:intron 20.0%
!! TATGTTATGTCTTTGAATTT+GGG + chr8_2:21664261-21664280 Msa1239740:intron 20.0%
!!! TTTGATACCAATTTAGATTT+TGG + chr8_2:21664478-21664497 Msa1239740:intron 20.0%
!!! TTGATACCAATTTAGATTTT+GGG + chr8_2:21664479-21664498 Msa1239740:intron 20.0%
!! TGAAAAATGTGTTTATAAGT+AGG - chr8_2:21664556-21664575 None:intergenic 20.0%
!!! TACTTATAAACACATTTTTC+AGG + chr8_2:21664555-21664574 Msa1239740:intron 20.0%
!! CATTATGTTATCAATAAGTT+GGG + chr8_2:21664607-21664626 Msa1239740:intron 20.0%
!! ATTATGTTATCAATAAGTTG+GGG + chr8_2:21664608-21664627 Msa1239740:intron 20.0%
!! TGATATATGAATGAACATTA+TGG + chr8_2:21665310-21665329 Msa1239740:intron 20.0%
!!! TTATCGTCATTATATTTTCT+TGG + chr8_2:21665523-21665542 Msa1239740:intron 20.0%
!! TGAATTTAAAGAAACTTACA+AGG - chr8_2:21665574-21665593 None:intergenic 20.0%
!! GTTATTGCATTATAAACAAT+TGG + chr8_2:21665734-21665753 Msa1239740:intron 20.0%
!! AGGACTCAAATTTAATATAT+AGG - chr8_2:21665848-21665867 None:intergenic 20.0%
!! TTGCATATTTAATAATCTTG+TGG + chr8_2:21666213-21666232 Msa1239740:intron 20.0%
!!! AAAAGTACCATATTTAGATA+TGG + chr8_2:21666352-21666371 Msa1239740:intron 20.0%
!!! CTTAATTTCTCCTTTTTTTA+TGG + chr8_2:21666492-21666511 Msa1239740:intron 20.0%
!! ATAATTTATTAGGAAAGACT+TGG + chr8_2:21666657-21666676 Msa1239740:intron 20.0%
!! TTCTATCAAAAACCATATTA+CGG - chr8_2:21666699-21666718 None:intergenic 20.0%
!! ACTAGTTAATAAAAGCAATT+AGG - chr8_2:21666989-21667008 None:intergenic 20.0%
!!! ATTCATTTTGATTTGGTTTT+TGG - chr8_2:21667036-21667055 None:intergenic 20.0%
!! ACCAAATCAAAATGAATAAT+AGG + chr8_2:21667039-21667058 Msa1239740:intron 20.0%
!!! TCCTATTATTCATTTTGATT+TGG - chr8_2:21667043-21667062 None:intergenic 20.0%
!! ATTTGAGAAAAAATGTTCTT+TGG + chr8_2:21667438-21667457 Msa1239740:CDS 20.0%
!! ATTGTTGAAAATAATATCAC+AGG + chr8_2:21667482-21667501 Msa1239740:three_prime_UTR 20.0%
!! TTGTTGAAAATAATATCACA+GGG + chr8_2:21667483-21667502 Msa1239740:three_prime_UTR 20.0%
!! ACAAAAGATCAAATGAATTT+GGG - chr8_2:21667538-21667557 None:intergenic 20.0%
! AAGAGTTATTGTGAGTATTA+AGG + chr8_2:21663149-21663168 Msa1239740:five_prime_UTR 25.0%
! AGAGTTATTGTGAGTATTAA+GGG + chr8_2:21663150-21663169 Msa1239740:five_prime_UTR 25.0%
!!! TTTGGTGGTTTTTTTGTAAT+GGG - chr8_2:21663303-21663322 None:intergenic 25.0%
!!! ATTTGGTGGTTTTTTTGTAA+TGG - chr8_2:21663304-21663323 None:intergenic 25.0%
! TTGAAATTGAAAATGGAGAA+TGG - chr8_2:21663508-21663527 None:intergenic 25.0%
!! TGATTTTCTTGAGAAAATGA+AGG - chr8_2:21663597-21663616 None:intergenic 25.0%
! TTGATTTCAATGTGTTTGTT+TGG + chr8_2:21663948-21663967 Msa1239740:intron 25.0%
! TGTTTGGTTGAATAAAATAG+AGG + chr8_2:21663964-21663983 Msa1239740:intron 25.0%
! AAAAAATAAGCCAATCCAAA+CGG - chr8_2:21664035-21664054 None:intergenic 25.0%
! TTTATAAGTTTGTCATGTGA+AGG + chr8_2:21664102-21664121 Msa1239740:intron 25.0%
! CAAAAATAAGTCAATCCAAA+CGG + chr8_2:21664228-21664247 Msa1239740:intron 25.0%
! AAAAATAAGTCAATCCAAAC+GGG + chr8_2:21664229-21664248 Msa1239740:intron 25.0%
! TTGAAAATGTGTTCATTAGT+AGG - chr8_2:21664336-21664355 None:intergenic 25.0%
! ATCTAAATTGGTATCAAACT+CGG - chr8_2:21664476-21664495 None:intergenic 25.0%
! CAAACTTAAACCTAGAAAAT+CGG + chr8_2:21664508-21664527 Msa1239740:intron 25.0%
! AAACTTAAACCTAGAAAATC+GGG + chr8_2:21664509-21664528 Msa1239740:intron 25.0%
! GCATTATGTTATCAATAAGT+TGG + chr8_2:21664606-21664625 Msa1239740:intron 25.0%
! TTGAAATGAATGTTTGTGTT+TGG + chr8_2:21664761-21664780 Msa1239740:intron 25.0%
! TGAAATGAATGTTTGTGTTT+GGG + chr8_2:21664762-21664781 Msa1239740:intron 25.0%
!! CTTTGTTTATTGATACAACA+AGG + chr8_2:21664915-21664934 Msa1239740:intron 25.0%
!! ATATCTTAAACTGATTGGTT+CGG + chr8_2:21665055-21665074 Msa1239740:intron 25.0%
! ACTTAATAATTCCTAGTTCA+TGG + chr8_2:21665123-21665142 Msa1239740:intron 25.0%
! TATGTTAGAATTAACATCGA+GGG - chr8_2:21665435-21665454 None:intergenic 25.0%
! ATATGTTAGAATTAACATCG+AGG - chr8_2:21665436-21665455 None:intergenic 25.0%
! TAGAAGGAAACAAGAAAAAA+GGG - chr8_2:21665659-21665678 None:intergenic 25.0%
! TTAGAAGGAAACAAGAAAAA+AGG - chr8_2:21665660-21665679 None:intergenic 25.0%
!!! ATATCCAAGACTATTTTTCT+AGG - chr8_2:21665793-21665812 None:intergenic 25.0%
! TTTCTCAATATTCAAAACTG+AGG - chr8_2:21665868-21665887 None:intergenic 25.0%
! GATAGAATAACGACTAAAAA+GGG - chr8_2:21665991-21666010 None:intergenic 25.0%
! TGATAGAATAACGACTAAAA+AGG - chr8_2:21665992-21666011 None:intergenic 25.0%
!! TTGATTTACTGTTGATTGAT+TGG - chr8_2:21666074-21666093 None:intergenic 25.0%
! CGGTTTAGTGATAATTTATT+AGG - chr8_2:21666294-21666313 None:intergenic 25.0%
! ATAAATTATCACTAAACCGT+CGG + chr8_2:21666295-21666314 Msa1239740:intron 25.0%
! ATAGGATCCATATCTAAATA+TGG - chr8_2:21666362-21666381 None:intergenic 25.0%
! CTTTCAATATCTGAAAAACT+AGG - chr8_2:21666414-21666433 None:intergenic 25.0%
!!! TTTCTCCTTTTTTTATGGTA+AGG + chr8_2:21666497-21666516 Msa1239740:intron 25.0%
!! TGGTTTTTGATAGAATGTTA+TGG + chr8_2:21666704-21666723 Msa1239740:intron 25.0%
!! TGCTTTTATTAACTAGTAGT+AGG + chr8_2:21666992-21667011 Msa1239740:intron 25.0%
!!! TAGTTTAGTTGTTTTTGATG+CGG + chr8_2:21667167-21667186 Msa1239740:intron 25.0%
! ATATTATTTCGACATTCTTG+AGG - chr8_2:21667290-21667309 None:intergenic 25.0%
! ATAAGTCGAATTCATTTGTA+TGG - chr8_2:21667399-21667418 None:intergenic 25.0%
! GACAAAAGATCAAATGAATT+TGG - chr8_2:21667539-21667558 None:intergenic 25.0%
TAAGGGATTATTTAAGCGTT+AGG + chr8_2:21663167-21663186 Msa1239740:five_prime_UTR 30.0%
AAGGGATTATTTAAGCGTTA+GGG + chr8_2:21663168-21663187 Msa1239740:five_prime_UTR 30.0%
ACTAACAAAGTGAACAATCA+GGG + chr8_2:21663200-21663219 Msa1239740:five_prime_UTR 30.0%
!!! TTGGTGGTTTTTTTGTAATG+GGG - chr8_2:21663302-21663321 None:intergenic 30.0%
!! AAGATTGATGGTGAAGATTA+GGG - chr8_2:21663448-21663467 None:intergenic 30.0%
AGGGGGAAAATTGAAAATTT+GGG - chr8_2:21663554-21663573 None:intergenic 30.0%
AAGGGGGAAAATTGAAAATT+TGG - chr8_2:21663555-21663574 None:intergenic 30.0%
TGAAGGAAAATGGAATGAAA+GGG - chr8_2:21663580-21663599 None:intergenic 30.0%
ATGAAGGAAAATGGAATGAA+AGG - chr8_2:21663581-21663600 None:intergenic 30.0%
CTTGAGAAAATGAAGGAAAA+TGG - chr8_2:21663590-21663609 None:intergenic 30.0%
!! TACCAATTTAGATTTTGGGT+TGG + chr8_2:21664483-21664502 Msa1239740:intron 30.0%
!! CGATTTTCTAGGTTTAAGTT+TGG - chr8_2:21664510-21664529 None:intergenic 30.0%
TCTTATCTAATGTAAGACTC+TGG + chr8_2:21664583-21664602 Msa1239740:intron 30.0%
CTTATCTAATGTAAGACTCT+GGG + chr8_2:21664584-21664603 Msa1239740:intron 30.0%
TATTGTGATATGATCGAAGA+TGG + chr8_2:21664654-21664673 Msa1239740:intron 30.0%
!! TGCAATTGTTTCTAATTGCT+TGG + chr8_2:21664878-21664897 Msa1239740:intron 30.0%
TAGATACTAAGACCTTGATT+AGG + chr8_2:21664938-21664957 Msa1239740:intron 30.0%
AGATACTAAGACCTTGATTA+GGG + chr8_2:21664939-21664958 Msa1239740:intron 30.0%
!! TTTTGAGTGCTAATACGTAA+TGG + chr8_2:21665031-21665050 Msa1239740:intron 30.0%
ATGGCATATCTTAAACTGAT+TGG + chr8_2:21665050-21665069 Msa1239740:intron 30.0%
TAATAATTCCTAGTTCATGG+TGG + chr8_2:21665126-21665145 Msa1239740:intron 30.0%
GACCTATAGATTCTGTAATA+AGG + chr8_2:21665185-21665204 Msa1239740:intron 30.0%
CTCCTTATTACAGAATCTAT+AGG - chr8_2:21665190-21665209 None:intergenic 30.0%
AATAAGGAGAGTAGATCAAA+TGG + chr8_2:21665201-21665220 Msa1239740:intron 30.0%
AGATATAGAGAGTAATGAGT+TGG + chr8_2:21665276-21665295 Msa1239740:intron 30.0%
TTCTAACATATCTGCTTCAA+AGG + chr8_2:21665445-21665464 Msa1239740:intron 30.0%
TCTAACATATCTGCTTCAAA+GGG + chr8_2:21665446-21665465 Msa1239740:intron 30.0%
! TCGTCATTATATTTTCTTGG+AGG + chr8_2:21665526-21665545 Msa1239740:intron 30.0%
TTATCAAGCAGACAATTAGA+AGG - chr8_2:21665675-21665694 None:intergenic 30.0%
CTAATTGTCTGCTTGATAAA+TGG + chr8_2:21665676-21665695 Msa1239740:intron 30.0%
!! AAGACTATTTTTCTAGGTTC+AGG - chr8_2:21665787-21665806 None:intergenic 30.0%
TGAACCTAGAAAAATAGTCT+TGG + chr8_2:21665786-21665805 Msa1239740:intron 30.0%
! GAAAAATAGTCTTGGATATC+TGG + chr8_2:21665794-21665813 Msa1239740:intron 30.0%
TGTGTGATAAGAGTAAAACA+TGG - chr8_2:21665891-21665910 None:intergenic 30.0%
ATTGAAGTATATCTCTGCAT+GGG + chr8_2:21666153-21666172 Msa1239740:intron 30.0%
TTTATTAGGATCCATGCATT+TGG - chr8_2:21666280-21666299 None:intergenic 30.0%
!! TTCTAAAAACAAAGTACCGA+CGG - chr8_2:21666314-21666333 None:intergenic 30.0%
! TATGGATCCTATTTTATCAC+TGG + chr8_2:21666370-21666389 Msa1239740:intron 30.0%
TATCTGAAAAACTAGGAGTA+TGG - chr8_2:21666407-21666426 None:intergenic 30.0%
GAAATTAAGCTTTCCTCAAA+GGG - chr8_2:21666481-21666500 None:intergenic 30.0%
AGAAATTAAGCTTTCCTCAA+AGG - chr8_2:21666482-21666501 None:intergenic 30.0%
CACTTCCTTACCATAAAAAA+AGG - chr8_2:21666505-21666524 None:intergenic 30.0%
!!! ACGATTGAGTTTTAATTCAG+AGG + chr8_2:21666805-21666824 Msa1239740:intron 30.0%
CTATATAGGTATTTCCCATT+CGG - chr8_2:21666912-21666931 None:intergenic 30.0%
AAAAGCAATTAGGTACAGTT+CGG - chr8_2:21666979-21666998 None:intergenic 30.0%
TTATTAACTAGTAGTAGGAC+AGG + chr8_2:21666997-21667016 Msa1239740:intron 30.0%
CTAACAAGTATGTTCAATCT+GGG - chr8_2:21667130-21667149 None:intergenic 30.0%
ACTAACAAGTATGTTCAATC+TGG - chr8_2:21667131-21667150 None:intergenic 30.0%
!!! AGTTTAGTTGTTTTTGATGC+GGG + chr8_2:21667168-21667187 Msa1239740:intron 30.0%
TTCAGACAAACAATGATAGA+AGG - chr8_2:21667239-21667258 None:intergenic 30.0%
AATAATATCACAGGGCAATT+TGG + chr8_2:21667491-21667510 Msa1239740:three_prime_UTR 30.0%
ATCAAATGAATTTGGGAAAC+AGG - chr8_2:21667531-21667550 None:intergenic 30.0%
GACTAACAAAGTGAACAATC+AGG + chr8_2:21663199-21663218 Msa1239740:five_prime_UTR 35.0%
!! GAAGATTGATGGTGAAGATT+AGG - chr8_2:21663449-21663468 None:intergenic 35.0%
ACTTGAATTGAATTGCAGTG+TGG - chr8_2:21663481-21663500 None:intergenic 35.0%
AAATGGAATGAAAGGGGAAA+GGG - chr8_2:21663573-21663592 None:intergenic 35.0%
AAAATGGAATGAAAGGGGAA+AGG - chr8_2:21663574-21663593 None:intergenic 35.0%
GAAGGAAAATGGAATGAAAG+GGG - chr8_2:21663579-21663598 None:intergenic 35.0%
!!! TTTCGTGTTTTTTCAGGTGA+AGG + chr8_2:21663648-21663667 Msa1239740:intron 35.0%
CATTGGGTTAAGATGTCATT+CGG + chr8_2:21663757-21663776 Msa1239740:CDS 35.0%
AAGATGTCATTCGGAGAATT+TGG + chr8_2:21663766-21663785 Msa1239740:CDS 35.0%
AAAATAGAGGTGACTAGAGT+AGG + chr8_2:21663977-21663996 Msa1239740:intron 35.0%
CTGACTTAACCCTATAAAAC+CGG + chr8_2:21664290-21664309 Msa1239740:intron 35.0%
ATACTCTAGATGACCAATTC+TGG - chr8_2:21664402-21664421 None:intergenic 35.0%
! CATCTAGAGTATAACATGAG+TGG + chr8_2:21664410-21664429 Msa1239740:intron 35.0%
GTCCAACCCAAAATCTAAAT+TGG - chr8_2:21664488-21664507 None:intergenic 35.0%
! TGTTATCAATAAGTTGGGGT+AGG + chr8_2:21664612-21664631 Msa1239740:intron 35.0%
!! AATTTTGACACCATTCAGCT+AGG - chr8_2:21664857-21664876 None:intergenic 35.0%
!! CAAAGGGTACTTAGTACATA+TGG + chr8_2:21665462-21665481 Msa1239740:intron 35.0%
AGTACATATGGAAGCAAAAG+TGG + chr8_2:21665474-21665493 Msa1239740:intron 35.0%
TCTAGGTTCAGGTATAAACA+CGG - chr8_2:21665776-21665795 None:intergenic 35.0%
CATTGAAGTATATCTCTGCA+TGG + chr8_2:21666152-21666171 Msa1239740:intron 35.0%
TCAGTAATAAACAGGGATGA+CGG - chr8_2:21666241-21666260 None:intergenic 35.0%
GCTGAAGTCAGTAATAAACA+GGG - chr8_2:21666248-21666267 None:intergenic 35.0%
! AAAAACTAGGAGTATGGTAG+TGG - chr8_2:21666401-21666420 None:intergenic 35.0%
! TAATGAGTTGGACCGTAATA+TGG + chr8_2:21666684-21666703 Msa1239740:intron 35.0%
! TTATCCCATTTAAAGTGGGT+TGG - chr8_2:21666749-21666768 None:intergenic 35.0%
AACCCACTTTAAATGGGATA+AGG + chr8_2:21666748-21666767 Msa1239740:intron 35.0%
! AGCCTTATCCCATTTAAAGT+GGG - chr8_2:21666753-21666772 None:intergenic 35.0%
! AAGCCTTATCCCATTTAAAG+TGG - chr8_2:21666754-21666773 None:intergenic 35.0%
ACTTTAAATGGGATAAGGCT+TGG + chr8_2:21666753-21666772 Msa1239740:intron 35.0%
AGGAAAACTAAACCAAGGTT+CGG + chr8_2:21666863-21666882 Msa1239740:intron 35.0%
CGGTTCATAAAGAATCAACT+CGG - chr8_2:21666959-21666978 None:intergenic 35.0%
TTAACAGTCTGACTCTGATA+CGG - chr8_2:21667086-21667105 None:intergenic 35.0%
!!! ATGCTTGTTTTGTAGTCTCA+AGG + chr8_2:21667318-21667337 Msa1239740:intron 35.0%
ATCTTTACCAGTGACTATGT+TGG + chr8_2:21667574-21667593 Msa1239740:three_prime_UTR 35.0%
!!! CTGAGAAGAAGTTTTGAAGT+TGG + chr8_2:21667662-21667681 Msa1239740:three_prime_UTR 35.0%
AGGTCATAATCGTTGTAGTT+TGG - chr8_2:21667767-21667786 None:intergenic 35.0%
TACAACGATTATGACCTTGT+TGG + chr8_2:21667770-21667789 Msa1239740:three_prime_UTR 35.0%
TCAGTTAGCAAAAACCAACA+AGG - chr8_2:21667787-21667806 None:intergenic 35.0%
TCATGTGTTTGTTCACTTTG+TGG + chr8_2:21667869-21667888 Msa1239740:three_prime_UTR 35.0%
! TGCTCTAAACTAAGACTGTT+TGG + chr8_2:21667914-21667933 Msa1239740:three_prime_UTR 35.0%
ATTGAATTGCAGTGTGGTGT+GGG - chr8_2:21663475-21663494 None:intergenic 40.0%
AATTGAATTGCAGTGTGGTG+TGG - chr8_2:21663476-21663495 None:intergenic 40.0%
AATGGAATGAAAGGGGAAAG+GGG - chr8_2:21663572-21663591 None:intergenic 40.0%
!!! TCGGTGTTTCGTGTTTTTTC+AGG + chr8_2:21663642-21663661 Msa1239740:intron 40.0%
!! TCCTGAAAAGTGCCTTGATA+AGG + chr8_2:21663820-21663839 Msa1239740:CDS 40.0%
!! CCTGAAAAGTGCCTTGATAA+GGG + chr8_2:21663821-21663840 Msa1239740:CDS 40.0%
TAACATCACGTCCCTTATCA+AGG - chr8_2:21663835-21663854 None:intergenic 40.0%
AAGAAGAAACTCACAGTCCA+AGG - chr8_2:21663868-21663887 None:intergenic 40.0%
!!! CTAGTTTTATTGGGTCCGTT+TGG + chr8_2:21664017-21664036 Msa1239740:intron 40.0%
TTTATTGGGTCCGTTTGGAT+TGG + chr8_2:21664022-21664041 Msa1239740:intron 40.0%
ACATAAATTAAGGGCCCGTT+TGG - chr8_2:21664246-21664265 None:intergenic 40.0%
!! CCTTACAAGCCGGTTTTATA+GGG - chr8_2:21664302-21664321 None:intergenic 40.0%
!! ACCTTACAAGCCGGTTTTAT+AGG - chr8_2:21664303-21664322 None:intergenic 40.0%
!! TATAAAACCGGCTTGTAAGG+TGG + chr8_2:21664302-21664321 Msa1239740:intron 40.0%
ATATTTGCCACCTTACAAGC+CGG - chr8_2:21664312-21664331 None:intergenic 40.0%
GAGTCTCATTGGATGAGATA+TGG - chr8_2:21664359-21664378 None:intergenic 40.0%
GTAGATGATCCGATAGATCT+TGG + chr8_2:21664443-21664462 Msa1239740:intron 40.0%
GGAGTCTATCCAAGATCTAT+CGG - chr8_2:21664455-21664474 None:intergenic 40.0%
TCGGCTGACAAATACAGAAA+TGG - chr8_2:21664706-21664725 None:intergenic 40.0%
TTGAATGCCTAACTCAGCTA+AGG - chr8_2:21664789-21664808 None:intergenic 40.0%
CAGCTAGGTTCAAGAGAAAA+AGG - chr8_2:21664842-21664861 None:intergenic 40.0%
TTCTCTTGAACCTAGCTGAA+TGG + chr8_2:21664844-21664863 Msa1239740:intron 40.0%
TTTCCTAGCATCCCTAATCA+AGG - chr8_2:21664953-21664972 None:intergenic 40.0%
ACGGTATGTTCTATTGTTGC+TGG + chr8_2:21664988-21665007 Msa1239740:intron 40.0%
AAGGAGAGTAGATCAAATGG+AGG + chr8_2:21665204-21665223 Msa1239740:intron 40.0%
AGGAGAGTAGATCAAATGGA+GGG + chr8_2:21665205-21665224 Msa1239740:intron 40.0%
AGTCAAATCACGAGAGAAAG+AGG + chr8_2:21665229-21665248 Msa1239740:intron 40.0%
!! TAGAGAGTAATGAGTTGGTG+AGG + chr8_2:21665281-21665300 Msa1239740:intron 40.0%
!! AGAGAGTAATGAGTTGGTGA+GGG + chr8_2:21665282-21665301 Msa1239740:intron 40.0%
GGCTGAAGTCAGTAATAAAC+AGG - chr8_2:21666249-21666268 None:intergenic 40.0%
! TCCATGCATTTGGTGATAAG+AGG - chr8_2:21666270-21666289 None:intergenic 40.0%
GGTGTGACCAGTGATAAAAT+AGG - chr8_2:21666380-21666399 None:intergenic 40.0%
ACTGTTAGATACGTTAGCTC+TGG + chr8_2:21666537-21666556 Msa1239740:intron 40.0%
AGACTTGGAGACTAATGAGT+TGG + chr8_2:21666672-21666691 Msa1239740:intron 40.0%
TGTAGCCAACCCACTTTAAA+TGG + chr8_2:21666741-21666760 Msa1239740:intron 40.0%
GTAGCCAACCCACTTTAAAT+GGG + chr8_2:21666742-21666761 Msa1239740:intron 40.0%
CAGGAAGGAAAACTAAACCA+AGG + chr8_2:21666858-21666877 Msa1239740:intron 40.0%
! TCTGTGTTTCTGAACCGAAT+GGG + chr8_2:21666895-21666914 Msa1239740:intron 40.0%
! TCTGACTCTGATACGGTTTT+TGG - chr8_2:21667079-21667098 None:intergenic 40.0%
GGAACTATCTGTCACAGTTT+GGG - chr8_2:21667109-21667128 None:intergenic 40.0%
! TCTACACCAAAAGTGCACAA+AGG + chr8_2:21667342-21667361 Msa1239740:CDS 40.0%
AAGGAGATGGATGATTACGT+CGG + chr8_2:21667361-21667380 Msa1239740:CDS 40.0%
TAGACTACCAACATAGTCAC+TGG - chr8_2:21667584-21667603 None:intergenic 40.0%
!! GTTGGTAGTCTAAATGCTTC+TGG + chr8_2:21667592-21667611 Msa1239740:three_prime_UTR 40.0%
TCTGCGATAACATCTTGTGT+TGG + chr8_2:21667829-21667848 Msa1239740:three_prime_UTR 40.0%
TGTGTTTGTTCACTTTGTGG+AGG + chr8_2:21667872-21667891 Msa1239740:three_prime_UTR 40.0%
TGACTACCTGCTACACATTC+AGG + chr8_2:21663259-21663278 Msa1239740:five_prime_UTR 45.0%
GACTACCTGCTACACATTCA+GGG + chr8_2:21663260-21663279 Msa1239740:five_prime_UTR 45.0%
TTCAGGGACCAAAGTGACTA+CGG + chr8_2:21663276-21663295 Msa1239740:five_prime_UTR 45.0%
ATGGAATGAAAGGGGAAAGG+GGG - chr8_2:21663571-21663590 None:intergenic 45.0%
ATGGCATCTTCGAAGCACAT+TGG + chr8_2:21663740-21663759 Msa1239740:CDS 45.0%
TGGCATCTTCGAAGCACATT+GGG + chr8_2:21663741-21663760 Msa1239740:CDS 45.0%
!! CCCTTATCAAGGCACTTTTC+AGG - chr8_2:21663824-21663843 None:intergenic 45.0%
!! CCCTATAAAACCGGCTTGTA+AGG + chr8_2:21664299-21664318 Msa1239740:intron 45.0%
!! GTGAGTGTTGAGAGTCTCAT+TGG - chr8_2:21664370-21664389 None:intergenic 45.0%
! CCTTACAAGCCCGATTTTCT+AGG - chr8_2:21664521-21664540 None:intergenic 45.0%
CCTAGAAAATCGGGCTTGTA+AGG + chr8_2:21664518-21664537 Msa1239740:intron 45.0%
! TTTGGGACCTTAGCTGAGTT+AGG + chr8_2:21664779-21664798 Msa1239740:intron 45.0%
AGACCTTGATTAGGGATGCT+AGG + chr8_2:21664947-21664966 Msa1239740:intron 45.0%
! CCTAGTTCATGGTGGAAACT+CGG + chr8_2:21665134-21665153 Msa1239740:intron 45.0%
AAACTCGGCTTAGATGGTCT+GGG + chr8_2:21665149-21665168 Msa1239740:intron 45.0%
TTAGATGGTCTGGGCATGTA+GGG + chr8_2:21665158-21665177 Msa1239740:intron 45.0%
GCCTCTTATCACCAAATGCA+TGG + chr8_2:21666266-21666285 Msa1239740:intron 45.0%
TAAGCTTTCCTCAAAGGGCA+AGG - chr8_2:21666476-21666495 None:intergenic 45.0%
AATTCAGAGGACTGCCTAGT+AGG + chr8_2:21666818-21666837 Msa1239740:intron 45.0%
! CTCTGTGTTTCTGAACCGAA+TGG + chr8_2:21666894-21666913 Msa1239740:intron 45.0%
GGGAACTATCTGTCACAGTT+TGG - chr8_2:21667110-21667129 None:intergenic 45.0%
GGCGATACATACTTGTCAGT+AGG - chr8_2:21667218-21667237 None:intergenic 45.0%
! CCATCTCCTTTGTGCACTTT+TGG - chr8_2:21667351-21667370 None:intergenic 45.0%
! CCAAAAGTGCACAAAGGAGA+TGG + chr8_2:21667348-21667367 Msa1239740:CDS 45.0%
CAAATGCTTCCTGCTCTTTG+CGG - chr8_2:21667423-21667442 None:intergenic 45.0%
CACTTTGTGGAGGAACAATG+TGG + chr8_2:21667882-21667901 Msa1239740:three_prime_UTR 45.0%
!!! ATGTTTTTTTATAATTTATT+AGG + chr8_2:21666647-21666666 Msa1239740:intron 5.0%
TTGGTCCCTGAATGTGTAGC+AGG - chr8_2:21663268-21663287 None:intergenic 50.0%
TAATGGGGCCGTAGTCACTT+TGG - chr8_2:21663287-21663306 None:intergenic 50.0%
GGTGTGGGAAGGAAGATTGA+TGG - chr8_2:21663460-21663479 None:intergenic 50.0%
AATTGCAGTGTGGTGTGGGA+AGG - chr8_2:21663471-21663490 None:intergenic 50.0%
AAACCCGATCCCAACATCAG+CGG + chr8_2:21663712-21663731 Msa1239740:CDS 50.0%
AACACCGCTGATGTTGGGAT+CGG - chr8_2:21663719-21663738 None:intergenic 50.0%
CCATCAACACCGCTGATGTT+GGG - chr8_2:21663724-21663743 None:intergenic 50.0%
GATGTTACTCGCTGTGTCCT+TGG + chr8_2:21663848-21663867 Msa1239740:CDS 50.0%
CTCTCAACACTCACTCCTCA+CGG + chr8_2:21664376-21664395 Msa1239740:intron 50.0%
CCGAGTTTCCACCATGAACT+AGG - chr8_2:21665137-21665156 None:intergenic 50.0%
TGGTGGAAACTCGGCTTAGA+TGG + chr8_2:21665143-21665162 Msa1239740:intron 50.0%
GAAACTCGGCTTAGATGGTC+TGG + chr8_2:21665148-21665167 Msa1239740:intron 50.0%
CTTAGATGGTCTGGGCATGT+AGG + chr8_2:21665157-21665176 Msa1239740:intron 50.0%
GGATGATTCCTTGCCCTTTG+AGG + chr8_2:21666465-21666484 Msa1239740:intron 50.0%
ACTGCCTAGTAGGCTGTTCA+TGG + chr8_2:21666828-21666847 Msa1239740:intron 50.0%
TGAGCCATGAACAGCCTACT+AGG - chr8_2:21666835-21666854 None:intergenic 50.0%
TAGTAGGCTGTTCATGGCTC+AGG + chr8_2:21666834-21666853 Msa1239740:intron 50.0%
!! TAAACCAAGGTTCGGTGCAC+TGG + chr8_2:21666871-21666890 Msa1239740:intron 50.0%
! TCGAGTGCTGTAGATGCTGC+TGG + chr8_2:21663689-21663708 Msa1239740:CDS 55.0%
ACACCGCTGATGTTGGGATC+GGG - chr8_2:21663718-21663737 None:intergenic 55.0%
! CCCAACATCAGCGGTGTTGA+TGG + chr8_2:21663721-21663740 Msa1239740:CDS 55.0%
GCCATCAACACCGCTGATGT+TGG - chr8_2:21663725-21663744 None:intergenic 55.0%
TCACTCCTCACGGCCAGAAT+TGG + chr8_2:21664386-21664405 Msa1239740:intron 55.0%
GATGACCAATTCTGGCCGTG+AGG - chr8_2:21664394-21664413 None:intergenic 55.0%
GTTCATGGCTCAGGTCAGGA+AGG + chr8_2:21666843-21666862 Msa1239740:intron 55.0%
CGACTTATGCCGCAAAGAGC+AGG + chr8_2:21667411-21667430 Msa1239740:CDS 55.0%
GGCTGTTCATGGCTCAGGTC+AGG + chr8_2:21666839-21666858 Msa1239740:intron 60.0%
AGAGCCAGTGCACCGAACCT+TGG - chr8_2:21666878-21666897 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 21663004 21667962 21663004 ID=Msa1239740;Name=Msa1239740
chr8_2 mRNA 21663004 21667962 21663004 ID=Msa1239740-mRNA-1;Parent=Msa1239740;Name=Msa1239740-mRNA-1;_AED=0.07;_eAED=0.07;_QI=517|1|1|1|0.5|0.33|3|497|106
chr8_2 exon 21663004 21663498 21663004 ID=Msa1239740-mRNA-1:exon:7750;Parent=Msa1239740-mRNA-1
chr8_2 exon 21663664 21663873 21663664 ID=Msa1239740-mRNA-1:exon:7751;Parent=Msa1239740-mRNA-1
chr8_2 exon 21667333 21667962 21667333 ID=Msa1239740-mRNA-1:exon:7752;Parent=Msa1239740-mRNA-1
chr8_2 five_prime_UTR 21663004 21663498 21663004 ID=Msa1239740-mRNA-1:five_prime_utr;Parent=Msa1239740-mRNA-1
chr8_2 five_prime_UTR 21663664 21663685 21663664 ID=Msa1239740-mRNA-1:five_prime_utr;Parent=Msa1239740-mRNA-1
chr8_2 CDS 21663686 21663873 21663686 ID=Msa1239740-mRNA-1:cds;Parent=Msa1239740-mRNA-1
chr8_2 CDS 21667333 21667465 21667333 ID=Msa1239740-mRNA-1:cds;Parent=Msa1239740-mRNA-1
chr8_2 three_prime_UTR 21667466 21667962 21667466 ID=Msa1239740-mRNA-1:three_prime_utr;Parent=Msa1239740-mRNA-1
Gene Sequence

>Msa1239740

ATGTCGAGTGCTGTAGATGCTGCTGGAAACCCGATCCCAACATCAGCGGTGTTGATGGCATCTTCGAAGCACATTGGGTTAAGATGTCATTCGGAGAATTTGGAGTTTCTGAAATGTAAGAAGAAAGATCAGAATCCTGAAAAGTGCCTTGATAAGGGACGTGATGTTACTCGCTGTGTCCTTGGACTTCTCAAGGATCTACACCAAAAGTGCACAAAGGAGATGGATGATTACGTCGGTTGCATGTACTACCATACAAATGAATTCGACTTATGCCGCAAAGAGCAGGAAGCATTTGAGAAAAAATGTTCTTTGGAATGA
Protein sequence

>Msa1239740

MSSAVDAAGNPIPTSAVLMASSKHIGLRCHSENLEFLKCKKKDQNPEKCLDKGRDVTRCVLGLLKDLHQKCTKEMDDYVGCMYYHTNEFDLCRKEQEAFEKKCSLE*