Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1241790 | AET02409.1 | 97.345 | 113 | 3 | 0 | 1 | 113 | 592 | 704 | 8.21e-65 | 223 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1241790 | sp|Q8RX56|UNC13_ARATH | 62.385 | 109 | 41 | 0 | 5 | 113 | 592 | 700 | 3.63e-41 | 147 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1241790 | G7LHI4 | 97.345 | 113 | 3 | 0 | 1 | 113 | 592 | 704 | 3.92e-65 | 223 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0008670 | Msa1241790 | 0.802439 | 5.608060e-49 | -8.615850e-47 |
| Msa1241790 | Msa1313150 | 0.811485 | 6.837750e-51 | -8.615850e-47 |
| Msa1241790 | Msa1423880 | 0.814509 | 1.485764e-51 | -8.615850e-47 |
| Msa0388960 | Msa1241790 | 0.810058 | 1.392141e-50 | -8.615850e-47 |
| Msa1082500 | Msa1241790 | 0.806455 | 8.156271e-50 | -8.615850e-47 |
| Msa0245940 | Msa1241790 | 0.812499 | 4.111484e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1241790 | MtrunA17_Chr8g0353581 | 97.345 | 113 | 3 | 0 | 1 | 113 | 592 | 704 | 8.51e-69 | 222 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1241790 | AT5G06970.1 | 62.385 | 109 | 41 | 0 | 5 | 113 | 592 | 700 | 3.69e-42 | 147 |
| Msa1241790 | AT4G11670.2 | 40.000 | 95 | 52 | 2 | 14 | 108 | 576 | 665 | 5.52e-13 | 64.3 |
| Msa1241790 | AT4G11670.3 | 39.773 | 88 | 51 | 1 | 14 | 101 | 576 | 661 | 5.96e-13 | 63.9 |
| Msa1241790 | AT4G11670.4 | 39.773 | 88 | 51 | 1 | 14 | 101 | 576 | 661 | 6.19e-13 | 63.9 |
| Msa1241790 | AT4G11670.1 | 39.773 | 88 | 51 | 1 | 14 | 101 | 571 | 656 | 6.75e-13 | 63.9 |
| Msa1241790 | AT2G20010.2 | 33.333 | 96 | 62 | 1 | 16 | 109 | 453 | 548 | 1.35e-11 | 60.1 |
| Msa1241790 | AT2G20010.1 | 33.333 | 96 | 62 | 1 | 16 | 109 | 335 | 430 | 1.48e-11 | 60.1 |
Find 16 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATTAGTTCACAAACTTTA+TGG | 0.122588 | 8_2:-25531161 | Msa1241790:CDS |
| CAAACTTTATGGTGTCAAAC+TGG | 0.381275 | 8_2:-25531150 | Msa1241790:intron |
| TGCTCTAGACTCTCAGCTGC+TGG | 0.423779 | 8_2:+25530987 | None:intergenic |
| GGATGTCTCTGTTGTAAAAC+AGG | 0.428231 | 8_2:+25531206 | None:intergenic |
| GAGTCTAGAGCAGTTTATAA+TGG | 0.451374 | 8_2:-25530976 | Msa1241790:CDS |
| CAATTTCCTCAACAAGATCT+CGG | 0.524136 | 8_2:+25530920 | None:intergenic |
| TTCTTCTGCAAGCAATGCCA+AGG | 0.549442 | 8_2:+25531268 | None:intergenic |
| TGTTCAGCACTATCCAGAAA+CGG | 0.573586 | 8_2:+25531038 | None:intergenic |
| TATGTCACATGAGCATCCCT+TGG | 0.582581 | 8_2:-25531285 | Msa1241790:CDS |
| GCTCTAGACTCTCAGCTGCT+GGG | 0.582829 | 8_2:+25530988 | None:intergenic |
| GAGGAAATTGAATCTCTACC+AGG | 0.590294 | 8_2:-25530907 | None:intergenic |
| AAAATGCCGAGATCTTGTTG+AGG | 0.642233 | 8_2:-25530926 | Msa1241790:CDS |
| GAGACATCCTCAAGCAACTG+TGG | 0.657830 | 8_2:-25531192 | Msa1241790:CDS |
| GCAGATACCACAGTTGCTTG+AGG | 0.666050 | 8_2:+25531185 | None:intergenic |
| TCTTCTGCAAGCAATGCCAA+GGG | 0.677560 | 8_2:+25531269 | None:intergenic |
| TAGTGCTGAACATTTAAGTG+AGG | 0.724046 | 8_2:-25531027 | Msa1241790:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGTTGCTGAATCTTTTTTT+AGG | + | chr8_2:25530988-25531007 | None:intergenic | 25.0% |
| ! | TCATTAGTTCACAAACTTTA+TGG | - | chr8_2:25531058-25531077 | Msa1241790:intron | 25.0% |
| !! | AGAATGACAGAAAAATCTTT+TGG | - | chr8_2:25531143-25531162 | Msa1241790:intron | 25.0% |
| ! | CAAACTTTATGGTGTCAAAC+TGG | - | chr8_2:25531069-25531088 | Msa1241790:intron | 35.0% |
| ! | TAGTGCTGAACATTTAAGTG+AGG | - | chr8_2:25531192-25531211 | Msa1241790:CDS | 35.0% |
| ! | GAGTCTAGAGCAGTTTATAA+TGG | - | chr8_2:25531243-25531262 | Msa1241790:CDS | 35.0% |
| CAATTTCCTCAACAAGATCT+CGG | + | chr8_2:25531302-25531321 | None:intergenic | 35.0% | |
| GGATGTCTCTGTTGTAAAAC+AGG | + | chr8_2:25531016-25531035 | None:intergenic | 40.0% | |
| ! | GTATTTTCAGAGACCGTTTC+TGG | - | chr8_2:25531168-25531187 | Msa1241790:CDS | 40.0% |
| TGTTCAGCACTATCCAGAAA+CGG | + | chr8_2:25531184-25531203 | None:intergenic | 40.0% | |
| ! | AGATCTCGGCATTTTCTTCA+TGG | + | chr8_2:25531288-25531307 | None:intergenic | 40.0% |
| AAAATGCCGAGATCTTGTTG+AGG | - | chr8_2:25531293-25531312 | Msa1241790:CDS | 40.0% | |
| TATGTCACATGAGCATCCCT+TGG | - | chr8_2:25530934-25530953 | Msa1241790:CDS | 45.0% | |
| TCTTCTGCAAGCAATGCCAA+GGG | + | chr8_2:25530953-25530972 | None:intergenic | 45.0% | |
| TTCTTCTGCAAGCAATGCCA+AGG | + | chr8_2:25530954-25530973 | None:intergenic | 45.0% | |
| GAGACATCCTCAAGCAACTG+TGG | - | chr8_2:25531027-25531046 | Msa1241790:CDS | 50.0% | |
| GCAGATACCACAGTTGCTTG+AGG | + | chr8_2:25531037-25531056 | None:intergenic | 50.0% | |
| ! | GCTCTAGACTCTCAGCTGCT+GGG | + | chr8_2:25531234-25531253 | None:intergenic | 55.0% |
| ! | TGCTCTAGACTCTCAGCTGC+TGG | + | chr8_2:25531235-25531254 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 25530908 | 25531333 | 25530908 | ID=Msa1241790;Name=Msa1241790 |
| chr8_2 | mRNA | 25530908 | 25531333 | 25530908 | ID=Msa1241790-mRNA-1;Parent=Msa1241790;Name=Msa1241790-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|1|0|1|1|1|2|0|113 |
| chr8_2 | exon | 25531151 | 25531333 | 25531151 | ID=Msa1241790-mRNA-1:exon:8991;Parent=Msa1241790-mRNA-1 |
| chr8_2 | exon | 25530908 | 25531063 | 25530908 | ID=Msa1241790-mRNA-1:exon:8990;Parent=Msa1241790-mRNA-1 |
| chr8_2 | CDS | 25531151 | 25531333 | 25531151 | ID=Msa1241790-mRNA-1:cds;Parent=Msa1241790-mRNA-1 |
| chr8_2 | CDS | 25530908 | 25531063 | 25530908 | ID=Msa1241790-mRNA-1:cds;Parent=Msa1241790-mRNA-1 |
| Gene Sequence |
| Protein sequence |
