AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1251340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1251340 MtrunA17_Chr8g0366581 99.574 235 1 0 1 235 1 235 1.10e-179 492
Msa1251340 MtrunA17_Chr5g0411151 84.874 238 32 3 1 235 1 237 2.96e-148 412
Msa1251340 MtrunA17_Chr5g0395181 69.767 215 56 3 19 233 7 212 2.06e-107 308
Msa1251340 MtrunA17_Chr4g0040071 72.059 204 53 4 31 233 13 213 5.25e-104 300
Msa1251340 MtrunA17_Chr1g0179781 55.340 206 73 5 33 229 7 202 5.98e-73 220
Msa1251340 MtrunA17_Chr7g0265291 58.333 204 71 5 33 229 7 203 1.86e-71 217
Msa1251340 MtrunA17_Chr2g0308401 49.265 136 59 4 34 159 5 140 6.06e-39 132
Msa1251340 MtrunA17_Chr4g0020811 47.794 136 61 3 34 159 7 142 8.51e-39 132
Msa1251340 MtrunA17_Chr4g0065041 48.031 127 65 1 33 159 8 133 6.51e-37 130
Msa1251340 MtrunA17_Chr2g0297251 34.685 222 118 5 33 228 37 257 8.86e-37 130
Msa1251340 MtrunA17_Chr2g0320521 34.052 232 109 6 34 228 7 231 3.29e-36 127
Msa1251340 MtrunA17_Chr7g0230511 32.922 243 117 5 30 228 6 246 4.66e-36 127
Msa1251340 MtrunA17_Chr2g0320561 46.715 137 63 4 34 160 5 141 6.39e-32 117
Msa1251340 MtrunA17_Chr2g0320551 43.382 136 67 3 34 159 5 140 4.53e-31 112
Msa1251340 MtrunA17_Chr4g0052451 43.902 82 40 1 85 160 11 92 3.36e-20 82.0
Msa1251340 MtrunA17_Chr8g0392151 33.951 162 82 6 19 159 1 158 1.26e-16 76.3
Msa1251340 MtrunA17_Chr8g0386511 29.193 161 99 4 72 228 4 153 2.31e-15 70.9
Msa1251340 MtrunA17_Chr5g0434331 27.670 206 105 9 29 228 39 206 1.10e-14 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1251340 AT4G17900.1 73.391 233 52 5 1 229 1 227 4.56e-122 346
Msa1251340 AT4G17900.2 72.906 203 46 4 30 229 17 213 1.13e-103 299
Msa1251340 AT1G32700.1 70.968 217 53 5 24 235 2 213 2.15e-103 298
Msa1251340 AT1G32700.2 70.391 179 44 4 61 235 1 174 5.88e-80 238
Msa1251340 AT5G46710.1 52.521 238 90 8 1 228 1 225 9.29e-78 234
Msa1251340 AT1G21000.2 50.209 239 82 4 26 228 6 243 6.88e-77 232
Msa1251340 AT1G21000.1 50.209 239 82 4 26 228 9 246 8.82e-77 232
Msa1251340 AT1G76590.1 50.877 228 80 4 33 228 18 245 2.10e-74 226
Msa1251340 AT5G46710.3 52.830 212 84 6 26 228 5 209 4.57e-72 219
Msa1251340 AT5G46710.2 59.627 161 57 3 1 159 1 155 1.43e-66 203
Msa1251340 AT1G43000.1 46.083 217 101 4 26 228 2 216 4.37e-62 194
Msa1251340 AT1G43000.2 46.083 217 101 4 26 228 9 223 4.71e-62 194
Msa1251340 AT2G27930.1 44.712 208 83 5 33 230 4 189 3.90e-53 170
Msa1251340 AT2G27930.4 44.712 208 83 5 33 230 4 189 3.90e-53 170
Msa1251340 AT2G27930.3 55.474 137 51 2 33 159 4 140 9.21e-49 158
Msa1251340 AT2G27930.2 55.474 137 51 2 33 159 4 140 9.21e-49 158
Msa1251340 AT2G12646.1 48.819 127 64 1 33 159 6 131 2.81e-37 131
Msa1251340 AT2G12646.2 48.819 127 64 1 33 159 6 131 2.81e-37 131
Msa1251340 AT2G12646.3 48.819 127 64 1 33 159 3 128 2.90e-37 131
Msa1251340 AT3G60670.1 46.212 132 70 1 28 159 2 132 9.59e-36 127
Msa1251340 AT1G31040.1 34.513 226 116 5 33 228 19 242 2.81e-34 123
Msa1251340 AT2G27930.5 45.455 154 53 4 86 230 61 192 5.55e-34 121
Msa1251340 AT2G27930.6 45.455 154 53 4 86 230 61 192 5.55e-34 121
Msa1251340 AT2G01818.1 34.579 214 115 9 35 228 13 221 4.23e-22 90.9

Find 84 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGAATTCCCTTTCTTCTTT+TGG 0.157849 8_2:-47296514 None:intergenic
CAAAGATCATCGTGCTATTC+AGG 0.214126 8_2:+47294970 Msa1251340:CDS
GGTAATGACAATAGTATATT+AGG 0.235473 8_2:+47297055 Msa1251340:three_prime_UTR
TGTATGTGTGTGTTGTTTAT+TGG 0.237468 8_2:-47293962 None:intergenic
AGTAAGTTATATGTAAGTTT+TGG 0.253096 8_2:+47297090 Msa1251340:three_prime_UTR
GAGCTGACTTGTTCTTCTTC+TGG 0.262316 8_2:-47296365 None:intergenic
TAGGGGAGCTCGACTCATTA+AGG 0.271314 8_2:-47296612 None:intergenic
CCTTGCACTGTTGATAATAT+AGG 0.282719 8_2:-47295562 None:intergenic
CTTCATACCATGATGTGATT+AGG 0.304121 8_2:+47295496 Msa1251340:CDS
TCCTTTCGATTCTGTTCTCT+TGG 0.334164 8_2:+47295672 Msa1251340:CDS
GTATGTGTGTGTTGTTTATT+GGG 0.334443 8_2:-47293961 None:intergenic
TAGTGTTTGTTTGTGTATAT+AGG 0.343461 8_2:+47296881 Msa1251340:three_prime_UTR
AACTATGAATTCTAGATTGT+TGG 0.377091 8_2:+47296327 Msa1251340:intron
TTACTCTTTACAAATATAGA+TGG 0.378670 8_2:+47297153 Msa1251340:three_prime_UTR
TTCATACCATGATGTGATTA+GGG 0.385029 8_2:+47295497 Msa1251340:CDS
TTCACAATTTCATACATAGA+TGG 0.388068 8_2:-47297177 None:intergenic
CAGAAAGTGTTGGACATAAC+AGG 0.395592 8_2:+47295531 Msa1251340:CDS
ACTGTTGGTGGTGGTGTTGA+AGG 0.411165 8_2:-47296477 None:intergenic
TATTGTTTGGATTGTATGAA+TGG 0.420599 8_2:+47294917 Msa1251340:CDS
TGAGTGCAATATGTATTGTT+TGG 0.429253 8_2:+47294904 Msa1251340:CDS
TGGAAGACTGAAGAACATTA+AGG 0.432710 8_2:+47296440 Msa1251340:CDS
ATCTGTGAGATACTATAGTT+TGG 0.437513 8_2:+47296945 Msa1251340:three_prime_UTR
TGTGTTTGATACAGATAAGA+AGG 0.447190 8_2:+47295472 Msa1251340:intron
GTCCTGATGATGAGGAAAAC+AGG 0.450549 8_2:+47294804 Msa1251340:CDS
GCAGCACTAGTCCTCCCATC+GGG 0.455937 8_2:-47296545 None:intergenic
GAGAAGTTTATAACTATTTG+AGG 0.456285 8_2:-47293874 None:intergenic
CACAGCAGCAGTTGCAGCCA+TGG 0.460151 8_2:+47296420 Msa1251340:CDS
TAATGTTCTTCAGTCTTCCA+TGG 0.466799 8_2:-47296437 None:intergenic
CAGATCAGCCAAAAGAAGAA+AGG 0.467780 8_2:+47296506 Msa1251340:CDS
GAGATTCGCATTCACGCAGT+TGG 0.470758 8_2:+47296641 Msa1251340:three_prime_UTR
ATCTATATTAAAGGCGAACT+CGG 0.478044 8_2:-47296760 None:intergenic
TGAGGCGGTGAAAGAGGGAA+TGG 0.479527 8_2:-47293856 None:intergenic
AGGGGAGCTCGACTCATTAA+GGG 0.483118 8_2:-47296611 None:intergenic
GATTCCACATTCACTTAGTC+GGG 0.484562 8_2:-47296720 None:intergenic
GAATAGCACGATGATCTTTG+TGG 0.487973 8_2:-47294967 None:intergenic
GCTGATCTGTAATTGACTGT+TGG 0.490334 8_2:-47296492 None:intergenic
CCTCAGCCAAGGCCCGGAAA+AGG 0.495211 8_2:+47295606 Msa1251340:CDS
GGATTCCACATTCACTTAGT+CGG 0.498426 8_2:-47296721 None:intergenic
CTCGGATTCCCTTCAGTCAA+GGG 0.499118 8_2:-47296742 None:intergenic
GAGGCGGTGAAAGAGGGAAT+GGG 0.500113 8_2:-47293855 None:intergenic
AGATCAGCCAAAAGAAGAAA+GGG 0.506864 8_2:+47296507 Msa1251340:CDS
AACGACAGCACTTCCAGCAC+TGG 0.512333 8_2:-47296856 None:intergenic
TTGTGATGAATGTGAGAAAG+TGG 0.518718 8_2:-47293992 None:intergenic
ATTCTGTTCTCTTGGCTGCA+AGG 0.521419 8_2:+47295680 Msa1251340:CDS
AATGCGAATCTCTAACTGTA+GGG 0.521833 8_2:-47296630 None:intergenic
CCTATATTATCAACAGTGCA+AGG 0.522886 8_2:+47295562 Msa1251340:CDS
GAAAGGCCTCAGCCAAGGCC+CGG 0.523082 8_2:+47295600 Msa1251340:CDS
ACTATTTGAGGCGGTGAAAG+AGG 0.528231 8_2:-47293862 None:intergenic
TTGGTGAAAAGGGCCAGTGC+TGG 0.531994 8_2:+47296843 Msa1251340:three_prime_UTR
GATCTGTAATTGACTGTTGG+TGG 0.533120 8_2:-47296489 None:intergenic
CTGTAATTGACTGTTGGTGG+TGG 0.534739 8_2:-47296486 None:intergenic
GGGTTGTGTTCTTGAATGAA+AGG 0.538393 8_2:+47295583 Msa1251340:CDS
TGCAGCACTAGTCCTCCCAT+CGG 0.541315 8_2:-47296546 None:intergenic
TTTGAACTATCAAACTTGAT+CGG 0.547763 8_2:-47296679 None:intergenic
GAATGCGAATCTCTAACTGT+AGG 0.552899 8_2:-47296631 None:intergenic
ATTCCGCATCGTGCCCCGAT+GGG 0.553697 8_2:+47296531 Msa1251340:CDS
AATTCCGCATCGTGCCCCGA+TGG 0.556752 8_2:+47296530 Msa1251340:CDS
CTCGCAGACTTCACAAGTGT+TGG 0.560178 8_2:-47295634 None:intergenic
CATTCACCCTAATCACATCA+TGG 0.571167 8_2:-47295503 None:intergenic
CCATCTTTAACTTAGTCCAA+AGG 0.571248 8_2:-47297004 None:intergenic
CCGCATCGTGCCCCGATGGG+AGG 0.573029 8_2:+47296534 Msa1251340:CDS
ACTCGGATTCCCTTCAGTCA+AGG 0.573463 8_2:-47296743 None:intergenic
CTATTTGAGGCGGTGAAAGA+GGG 0.577524 8_2:-47293861 None:intergenic
TTGAGCAGAGGTTTCAACCA+TGG 0.583253 8_2:-47294833 None:intergenic
AAGAAACGCTCTTTGAGCAG+AGG 0.583635 8_2:-47294845 None:intergenic
GAATGAGATTCAGAAAGTGT+TGG 0.585916 8_2:+47295521 Msa1251340:CDS
TGTGGAATCCCTTGACTGAA+GGG 0.591725 8_2:+47296734 Msa1251340:three_prime_UTR
TGTGATGAATGTGAGAAAGT+GGG 0.593199 8_2:-47293991 None:intergenic
ATGTGGAATCCCTTGACTGA+AGG 0.593619 8_2:+47296733 Msa1251340:three_prime_UTR
AGGAAAACAGGTGGCCACCA+TGG 0.603560 8_2:+47294816 Msa1251340:CDS
TGAATGAAAGGCCTCAGCCA+AGG 0.624070 8_2:+47295595 Msa1251340:CDS
CCTCCCATCGGGGCACGATG+CGG 0.625934 8_2:-47296534 None:intergenic
GCCAAGAGAACAGAATCGAA+AGG 0.631094 8_2:-47295673 None:intergenic
AGCAGAGGTTTCAACCATGG+TGG 0.633067 8_2:-47294830 None:intergenic
AGAATCGAAAGGAATCGAGA+AGG 0.635045 8_2:-47295662 None:intergenic
TGCTGGAGGTCCTGATGATG+AGG 0.642425 8_2:+47294796 Msa1251340:CDS
TTCAATGATATCAGACTCAG+AGG 0.648076 8_2:+47296392 Msa1251340:CDS
ATGTCCCGACTAAGTGAATG+TGG 0.655624 8_2:+47296716 Msa1251340:three_prime_UTR
CTATATTATCAACAGTGCAA+GGG 0.657853 8_2:+47295563 Msa1251340:CDS
ATGCGAATCTCTAACTGTAG+GGG 0.665946 8_2:-47296629 None:intergenic
AAGTTTATAACTATTTGAGG+CGG 0.679846 8_2:-47293871 None:intergenic
TCACTTAGTCGGGACATCAG+TGG 0.682173 8_2:-47296710 None:intergenic
CTGATGATGAGGAAAACAGG+TGG 0.717367 8_2:+47294807 Msa1251340:CDS
CAGCACTAGTCCTCCCATCG+GGG 0.730442 8_2:-47296544 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTTTATAAAAAAATT+GGG + chr8_2:47294526-47294545 Msa1251340:intron 0.0%
!!! AAAATTTGATTTTTTTTACA+CGG + chr8_2:47294288-47294307 Msa1251340:intron 10.0%
!!! TTAATTTTTTGTTTTGTTTT+TGG + chr8_2:47294380-47294399 Msa1251340:intron 10.0%
!!! TAATTATTTTTAAGTTAACT+TGG + chr8_2:47294658-47294677 Msa1251340:intron 10.0%
!!! TTTTGATAATTTTTTTTTGA+AGG + chr8_2:47294772-47294791 Msa1251340:intron 10.0%
!!! TTTGATAATTTTTTTTTGAA+GGG + chr8_2:47294773-47294792 Msa1251340:intron 10.0%
!!! ATTATTATTACTACTATTTT+GGG - chr8_2:47295765-47295784 None:intergenic 10.0%
!!! TTATTTCTGTTTTTAAATTC+TGG + chr8_2:47294422-47294441 Msa1251340:intron 15.0%
!!! TATTTCTGTTTTTAAATTCT+GGG + chr8_2:47294423-47294442 Msa1251340:intron 15.0%
!!! TTTTATAAAAAAATTGACAC+TGG - chr8_2:47294521-47294540 None:intergenic 15.0%
!!! TTTTAGAGAGAAAAAAAATT+AGG + chr8_2:47294695-47294714 Msa1251340:intron 15.0%
!!! GATTATTATTACTACTATTT+TGG - chr8_2:47295766-47295785 None:intergenic 15.0%
!!! TTGATCAATATTTTAAATAG+TGG - chr8_2:47296047-47296066 None:intergenic 15.0%
!!! CTATTTAAAATATTGATCAA+TGG + chr8_2:47296047-47296066 Msa1251340:intron 15.0%
!! AAAAAACAGTTAATAATCAT+AGG + chr8_2:47296574-47296593 Msa1251340:exon 15.0%
!!! TTTTTTCGTATCTATATTAA+AGG - chr8_2:47296772-47296791 None:intergenic 15.0%
!!! TTGTTTGTGTTTTTGAATTT+TGG + chr8_2:47294256-47294275 Msa1251340:intron 20.0%
!!! TGTTTGTGTTTTTGAATTTT+GGG + chr8_2:47294257-47294276 Msa1251340:intron 20.0%
!! AAATAATAAACACACAATGA+AGG - chr8_2:47294336-47294355 None:intergenic 20.0%
!!! TTTTTGGAATGTAATGTTTT+TGG + chr8_2:47294396-47294415 Msa1251340:intron 20.0%
!!! TTTTGGAATGTAATGTTTTT+GGG + chr8_2:47294397-47294416 Msa1251340:intron 20.0%
!!! TTATTTGTGATTTTGAATTG+TGG + chr8_2:47294737-47294756 Msa1251340:intron 20.0%
!!! TATTTGTGATTTTGAATTGT+GGG + chr8_2:47294738-47294757 Msa1251340:intron 20.0%
!! AATAATTGAATTGATTGTAG+TGG - chr8_2:47295306-47295325 None:intergenic 20.0%
!!! ATTTGTTTTGATTGCAATTT+TGG + chr8_2:47295870-47295889 Msa1251340:intron 20.0%
!! TATTATAGACTATTCTTATG+AGG - chr8_2:47295952-47295971 None:intergenic 20.0%
!!! TCTTGTATTTTAGAGAATTA+CGG - chr8_2:47295986-47296005 None:intergenic 20.0%
!! ATAATCATGAATGCTAATAT+AGG + chr8_2:47296141-47296160 Msa1251340:intron 20.0%
!! TTGAAATGACAAAATTTCAA+AGG - chr8_2:47296204-47296223 None:intergenic 20.0%
!!! GAAATTTTGTCATTTCAATA+TGG + chr8_2:47296206-47296225 Msa1251340:intron 20.0%
!! AAATTGAATTGAATGCAAAA+GGG - chr8_2:47296233-47296252 None:intergenic 20.0%
!! TAAATTGAATTGAATGCAAA+AGG - chr8_2:47296234-47296253 None:intergenic 20.0%
!! TATAGATACGAAAAAATCTA+AGG + chr8_2:47296776-47296795 Msa1251340:three_prime_UTR 20.0%
!! TATAGTTTGGTAAAAAGAAA+TGG + chr8_2:47296958-47296977 Msa1251340:three_prime_UTR 20.0%
!! TGATCAAAAAAAGTTAAAGA+TGG + chr8_2:47297034-47297053 Msa1251340:intron 20.0%
!!! AGTAAGTTATATGTAAGTTT+TGG + chr8_2:47297090-47297109 Msa1251340:three_prime_UTR 20.0%
!!! GTAAGTTATATGTAAGTTTT+GGG + chr8_2:47297091-47297110 Msa1251340:three_prime_UTR 20.0%
!!! TAAGTTATATGTAAGTTTTG+GGG + chr8_2:47297092-47297111 Msa1251340:three_prime_UTR 20.0%
!! TTACTCTTTACAAATATAGA+TGG + chr8_2:47297153-47297172 Msa1251340:three_prime_UTR 20.0%
! AAGTTTATAACTATTTGAGG+CGG - chr8_2:47293874-47293893 None:intergenic 25.0%
! GAGAAGTTTATAACTATTTG+AGG - chr8_2:47293877-47293896 None:intergenic 25.0%
! TATTGTTTGGATTGTATGAA+TGG + chr8_2:47294917-47294936 Msa1251340:CDS 25.0%
! AACACAATAAATGTGTCAAA+GGG - chr8_2:47295072-47295091 None:intergenic 25.0%
! AATGGGAATTTATGGATAAT+TGG - chr8_2:47295170-47295189 None:intergenic 25.0%
! GAACAATAAATGGGAATTTA+TGG - chr8_2:47295178-47295197 None:intergenic 25.0%
! ATGACAAAATTTCAAAGGTT+GGG - chr8_2:47296199-47296218 None:intergenic 25.0%
! AATGACAAAATTTCAAAGGT+TGG - chr8_2:47296200-47296219 None:intergenic 25.0%
!!! TAACTTTTGAGTTTATGCAT+TGG + chr8_2:47296290-47296309 Msa1251340:intron 25.0%
! AACTATGAATTCTAGATTGT+TGG + chr8_2:47296327-47296346 Msa1251340:intron 25.0%
! TTTGAACTATCAAACTTGAT+CGG - chr8_2:47296682-47296701 None:intergenic 25.0%
! TAGTGTTTGTTTGTGTATAT+AGG + chr8_2:47296881-47296900 Msa1251340:three_prime_UTR 25.0%
! GTATATAGGAAAAATCCAAT+AGG + chr8_2:47296895-47296914 Msa1251340:three_prime_UTR 25.0%
! AAATAAGCAAAAAGTTCCTT+TGG + chr8_2:47296988-47297007 Msa1251340:three_prime_UTR 25.0%
! GGTAATGACAATAGTATATT+AGG + chr8_2:47297055-47297074 Msa1251340:three_prime_UTR 25.0%
! TTCACAATTTCATACATAGA+TGG - chr8_2:47297180-47297199 None:intergenic 25.0%
!! ATTTTGTAGAACTTTGAAGG+CGG - chr8_2:47293835-47293854 None:intergenic 30.0%
!! GTATGTGTGTGTTGTTTATT+GGG - chr8_2:47293964-47293983 None:intergenic 30.0%
!! TGTATGTGTGTGTTGTTTAT+TGG - chr8_2:47293965-47293984 None:intergenic 30.0%
TCCAATCAAAAACTTCAAGA+TGG + chr8_2:47294038-47294057 Msa1251340:exon 30.0%
!!! TGTTTTTGGGCTTAATTTCA+CGG - chr8_2:47294086-47294105 None:intergenic 30.0%
! ATTGGGAATGCAATGATTTT+AGG + chr8_2:47294543-47294562 Msa1251340:intron 30.0%
!!! AATTTTTTTTTGAAGGGTGC+TGG + chr8_2:47294779-47294798 Msa1251340:intron 30.0%
TGAGTGCAATATGTATTGTT+TGG + chr8_2:47294904-47294923 Msa1251340:CDS 30.0%
CACAACTTTGATGAATTCAA+TGG - chr8_2:47295013-47295032 None:intergenic 30.0%
GAACACAATAAATGTGTCAA+AGG - chr8_2:47295073-47295092 None:intergenic 30.0%
! GTTTTTCTGACAACAAAAAC+AGG - chr8_2:47295095-47295114 None:intergenic 30.0%
!! AAAAACCATCACTTTTCCTT+TGG + chr8_2:47295109-47295128 Msa1251340:intron 30.0%
TAGAAATTGAAAACAGCACA+AGG - chr8_2:47295142-47295161 None:intergenic 30.0%
! AGCTTTTCAATGCAATAACA+TGG + chr8_2:47295419-47295438 Msa1251340:intron 30.0%
AAAAACAGAGTTCATTGGAA+AGG - chr8_2:47295450-47295469 None:intergenic 30.0%
ACACAAAAAACAGAGTTCAT+TGG - chr8_2:47295455-47295474 None:intergenic 30.0%
TGTGTTTGATACAGATAAGA+AGG + chr8_2:47295472-47295491 Msa1251340:intron 30.0%
TTCATACCATGATGTGATTA+GGG + chr8_2:47295497-47295516 Msa1251340:CDS 30.0%
CTATATTATCAACAGTGCAA+GGG + chr8_2:47295563-47295582 Msa1251340:CDS 30.0%
TCTTGTTCAAATGGTTAGAA+GGG - chr8_2:47295736-47295755 None:intergenic 30.0%
ATCTTGTTCAAATGGTTAGA+AGG - chr8_2:47295737-47295756 None:intergenic 30.0%
TGATTTAACGACCAAAAGTT+TGG - chr8_2:47295794-47295813 None:intergenic 30.0%
! AACTTTTGGTCGTTAAATCA+AGG + chr8_2:47295794-47295813 Msa1251340:intron 30.0%
AAATTTCAAAGGTTGGGTAT+AGG - chr8_2:47296193-47296212 None:intergenic 30.0%
ATCTATATTAAAGGCGAACT+CGG - chr8_2:47296763-47296782 None:intergenic 30.0%
ATCTGTGAGATACTATAGTT+TGG + chr8_2:47296945-47296964 Msa1251340:three_prime_UTR 30.0%
TTGGTAAAAAGAAATGGTGT+AGG + chr8_2:47296964-47296983 Msa1251340:three_prime_UTR 30.0%
! GGGATTTTGTAGAACTTTGA+AGG - chr8_2:47293838-47293857 None:intergenic 35.0%
TGTGATGAATGTGAGAAAGT+GGG - chr8_2:47293994-47294013 None:intergenic 35.0%
TTGTGATGAATGTGAGAAAG+TGG - chr8_2:47293995-47294014 None:intergenic 35.0%
! GCCATCTTGAAGTTTTTGAT+TGG - chr8_2:47294042-47294061 None:intergenic 35.0%
GTGAAATTAAGCCCAAAAAC+AGG + chr8_2:47294085-47294104 Msa1251340:CDS 35.0%
!! ACCATGATTCTCCTGTTTTT+GGG - chr8_2:47294099-47294118 None:intergenic 35.0%
! TACCATGATTCTCCTGTTTT+TGG - chr8_2:47294100-47294119 None:intergenic 35.0%
CAAATCCAAAGGAAAAGTGA+TGG - chr8_2:47295117-47295136 None:intergenic 35.0%
!! CATCACTTTTCCTTTGGATT+TGG + chr8_2:47295115-47295134 Msa1251340:intron 35.0%
GGTGGTTACGAACAATAAAT+GGG - chr8_2:47295187-47295206 None:intergenic 35.0%
TGGTGGTTACGAACAATAAA+TGG - chr8_2:47295188-47295207 None:intergenic 35.0%
TTGACACCAACATCAAATGT+CGG - chr8_2:47295270-47295289 None:intergenic 35.0%
! ATTAGTGTCATTGTTGTGTC+TGG + chr8_2:47295354-47295373 Msa1251340:intron 35.0%
CTTCATACCATGATGTGATT+AGG + chr8_2:47295496-47295515 Msa1251340:CDS 35.0%
! GAATGAGATTCAGAAAGTGT+TGG + chr8_2:47295521-47295540 Msa1251340:CDS 35.0%
!! CCTTGCACTGTTGATAATAT+AGG - chr8_2:47295565-47295584 None:intergenic 35.0%
CCTATATTATCAACAGTGCA+AGG + chr8_2:47295562-47295581 Msa1251340:CDS 35.0%
CTTGTTCAAATGGTTAGAAG+GGG - chr8_2:47295735-47295754 None:intergenic 35.0%
GGGAGTAAATCTTGTTCAAA+TGG - chr8_2:47295745-47295764 None:intergenic 35.0%
! ATAATCCACCACCAAACTTT+TGG + chr8_2:47295780-47295799 Msa1251340:intron 35.0%
TTTAACGACCAAAAGTTTGG+TGG - chr8_2:47295791-47295810 None:intergenic 35.0%
ACCTCAATGTTAATGCATTG+AGG + chr8_2:47295904-47295923 Msa1251340:intron 35.0%
ACCTCAATGCATTAACATTG+AGG - chr8_2:47295908-47295927 None:intergenic 35.0%
TTCAAGCTAGCACTTAAAAG+TGG + chr8_2:47296105-47296124 Msa1251340:intron 35.0%
TCAAGCTAGCACTTAAAAGT+GGG + chr8_2:47296106-47296125 Msa1251340:intron 35.0%
TTCAATGATATCAGACTCAG+AGG + chr8_2:47296392-47296411 Msa1251340:CDS 35.0%
TAATGTTCTTCAGTCTTCCA+TGG - chr8_2:47296440-47296459 None:intergenic 35.0%
TGGAAGACTGAAGAACATTA+AGG + chr8_2:47296440-47296459 Msa1251340:CDS 35.0%
AGATCAGCCAAAAGAAGAAA+GGG + chr8_2:47296507-47296526 Msa1251340:CDS 35.0%
AATGCGAATCTCTAACTGTA+GGG - chr8_2:47296633-47296652 None:intergenic 35.0%
!! CTAGAGCTTTTTGGTGAAAA+GGG + chr8_2:47296833-47296852 Msa1251340:three_prime_UTR 35.0%
TAGGAAAAATCCAATAGGAG+TGG + chr8_2:47296900-47296919 Msa1251340:three_prime_UTR 35.0%
!! CTAACTTTTTCCACTCCTAT+TGG - chr8_2:47296913-47296932 None:intergenic 35.0%
CAATAGGAGTGGAAAAAGTT+AGG + chr8_2:47296911-47296930 Msa1251340:three_prime_UTR 35.0%
! CCATCTTTAACTTAGTCCAA+AGG - chr8_2:47297007-47297026 None:intergenic 35.0%
CCTTTGGACTAAGTTAAAGA+TGG + chr8_2:47297004-47297023 Msa1251340:intron 35.0%
!!! AGTTTTGGGGATGTAAATAG+TGG + chr8_2:47297105-47297124 Msa1251340:three_prime_UTR 35.0%
TTAGCTCACACTCTCATTGT+TGG + chr8_2:47294166-47294185 Msa1251340:intron 40.0%
!!! TTTTTTTTGAAGGGTGCTGG+AGG + chr8_2:47294782-47294801 Msa1251340:intron 40.0%
GAATAGCACGATGATCTTTG+TGG - chr8_2:47294970-47294989 None:intergenic 40.0%
! CAAAGATCATCGTGCTATTC+AGG + chr8_2:47294970-47294989 Msa1251340:CDS 40.0%
CAATCTAAACCATCGATGTG+TGG - chr8_2:47295208-47295227 None:intergenic 40.0%
!! TTGATGTTGGTGTCAAATGC+CGG + chr8_2:47295274-47295293 Msa1251340:intron 40.0%
TTGATTGTAGTGGCATACAC+CGG - chr8_2:47295296-47295315 None:intergenic 40.0%
CATTCACCCTAATCACATCA+TGG - chr8_2:47295506-47295525 None:intergenic 40.0%
!! CAGAAAGTGTTGGACATAAC+AGG + chr8_2:47295531-47295550 Msa1251340:CDS 40.0%
GGGTTGTGTTCTTGAATGAA+AGG + chr8_2:47295583-47295602 Msa1251340:CDS 40.0%
AGAATCGAAAGGAATCGAGA+AGG - chr8_2:47295665-47295684 None:intergenic 40.0%
TCCTTTCGATTCTGTTCTCT+TGG + chr8_2:47295672-47295691 Msa1251340:CDS 40.0%
AAGGGGAAGACATTATGTGA+AGG - chr8_2:47295718-47295737 None:intergenic 40.0%
AGGTTGGGTATAGGAACAAA+TGG - chr8_2:47296184-47296203 None:intergenic 40.0%
GATCTGTAATTGACTGTTGG+TGG - chr8_2:47296492-47296511 None:intergenic 40.0%
GCTGATCTGTAATTGACTGT+TGG - chr8_2:47296495-47296514 None:intergenic 40.0%
CAGATCAGCCAAAAGAAGAA+AGG + chr8_2:47296506-47296525 Msa1251340:CDS 40.0%
! CGGAATTCCCTTTCTTCTTT+TGG - chr8_2:47296517-47296536 None:intergenic 40.0%
ATGCGAATCTCTAACTGTAG+GGG - chr8_2:47296632-47296651 None:intergenic 40.0%
GAATGCGAATCTCTAACTGT+AGG - chr8_2:47296634-47296653 None:intergenic 40.0%
GATTCCACATTCACTTAGTC+GGG - chr8_2:47296723-47296742 None:intergenic 40.0%
GGATTCCACATTCACTTAGT+CGG - chr8_2:47296724-47296743 None:intergenic 40.0%
!! GCTAGAGCTTTTTGGTGAAA+AGG + chr8_2:47296832-47296851 Msa1251340:three_prime_UTR 40.0%
! CTATTTGAGGCGGTGAAAGA+GGG - chr8_2:47293864-47293883 None:intergenic 45.0%
! ACTATTTGAGGCGGTGAAAG+AGG - chr8_2:47293865-47293884 None:intergenic 45.0%
GCCCAAAAACAGGAGAATCA+TGG + chr8_2:47294095-47294114 Msa1251340:CDS 45.0%
! GTCCTGATGATGAGGAAAAC+AGG + chr8_2:47294804-47294823 Msa1251340:CDS 45.0%
! CACCTGTTTTCCTCATCATC+AGG - chr8_2:47294809-47294828 None:intergenic 45.0%
! CTGATGATGAGGAAAACAGG+TGG + chr8_2:47294807-47294826 Msa1251340:CDS 45.0%
TTGAGCAGAGGTTTCAACCA+TGG - chr8_2:47294836-47294855 None:intergenic 45.0%
AAGAAACGCTCTTTGAGCAG+AGG - chr8_2:47294848-47294867 None:intergenic 45.0%
AGCACAAGGACCAAATCCAA+AGG - chr8_2:47295128-47295147 None:intergenic 45.0%
TCTAAACCATCGATGTGTGG+TGG - chr8_2:47295205-47295224 None:intergenic 45.0%
TGTCTGACACCAACATGTGT+CGG - chr8_2:47295247-47295266 None:intergenic 45.0%
CAGACACCGACATTTGATGT+TGG + chr8_2:47295261-47295280 Msa1251340:intron 45.0%
!!! AGTGTTGGTGACACCTTTTC+CGG - chr8_2:47295622-47295641 None:intergenic 45.0%
GCCAAGAGAACAGAATCGAA+AGG - chr8_2:47295676-47295695 None:intergenic 45.0%
ATTCTGTTCTCTTGGCTGCA+AGG + chr8_2:47295680-47295699 Msa1251340:CDS 45.0%
! AACGACCAAAAGTTTGGTGG+TGG - chr8_2:47295788-47295807 None:intergenic 45.0%
!! GAGCTGACTTGTTCTTCTTC+TGG - chr8_2:47296368-47296387 None:intergenic 45.0%
! CTGTAATTGACTGTTGGTGG+TGG - chr8_2:47296489-47296508 None:intergenic 45.0%
ATGTCCCGACTAAGTGAATG+TGG + chr8_2:47296716-47296735 Msa1251340:three_prime_UTR 45.0%
ATGTGGAATCCCTTGACTGA+AGG + chr8_2:47296733-47296752 Msa1251340:three_prime_UTR 45.0%
TGTGGAATCCCTTGACTGAA+GGG + chr8_2:47296734-47296753 Msa1251340:three_prime_UTR 45.0%
! GTTCCAGTGCTAGAGCTTTT+TGG + chr8_2:47296824-47296843 Msa1251340:three_prime_UTR 45.0%
! TCACCAAAAAGCTCTAGCAC+TGG - chr8_2:47296830-47296849 None:intergenic 45.0%
!!! CAATTTTTTTATAAAAAAAT+TGG + chr8_2:47294525-47294544 Msa1251340:intron 5.0%
AGCAGAGGTTTCAACCATGG+TGG - chr8_2:47294833-47294852 None:intergenic 50.0%
TCGTAACCACCACACATCGA+TGG + chr8_2:47295196-47295215 Msa1251340:intron 50.0%
GTTCACTGTCCGACACATGT+TGG + chr8_2:47295235-47295254 Msa1251340:intron 50.0%
TGAATGAAAGGCCTCAGCCA+AGG + chr8_2:47295595-47295614 Msa1251340:CDS 50.0%
!!! GTGTTGGTGACACCTTTTCC+GGG - chr8_2:47295621-47295640 None:intergenic 50.0%
CTCGCAGACTTCACAAGTGT+TGG - chr8_2:47295637-47295656 None:intergenic 50.0%
!! ACTGTTGGTGGTGGTGTTGA+AGG - chr8_2:47296480-47296499 None:intergenic 50.0%
AGGGGAGCTCGACTCATTAA+GGG - chr8_2:47296614-47296633 None:intergenic 50.0%
TAGGGGAGCTCGACTCATTA+AGG - chr8_2:47296615-47296634 None:intergenic 50.0%
GAGATTCGCATTCACGCAGT+TGG + chr8_2:47296641-47296660 Msa1251340:three_prime_UTR 50.0%
TCACTTAGTCGGGACATCAG+TGG - chr8_2:47296713-47296732 None:intergenic 50.0%
CTCGGATTCCCTTCAGTCAA+GGG - chr8_2:47296745-47296764 None:intergenic 50.0%
ACTCGGATTCCCTTCAGTCA+AGG - chr8_2:47296746-47296765 None:intergenic 50.0%
GAGGCGGTGAAAGAGGGAAT+GGG - chr8_2:47293858-47293877 None:intergenic 55.0%
TGAGGCGGTGAAAGAGGGAA+TGG - chr8_2:47293859-47293878 None:intergenic 55.0%
! TGCTGGAGGTCCTGATGATG+AGG + chr8_2:47294796-47294815 Msa1251340:CDS 55.0%
AGGAAAACAGGTGGCCACCA+TGG + chr8_2:47294816-47294835 Msa1251340:CDS 55.0%
TGCAGCACTAGTCCTCCCAT+CGG - chr8_2:47296549-47296568 None:intergenic 55.0%
TTGGTGAAAAGGGCCAGTGC+TGG + chr8_2:47296843-47296862 Msa1251340:three_prime_UTR 55.0%
AACGACAGCACTTCCAGCAC+TGG - chr8_2:47296859-47296878 None:intergenic 55.0%
! GTGACACCTTTTCCGGGCCT+TGG - chr8_2:47295615-47295634 None:intergenic 60.0%
CACAGCAGCAGTTGCAGCCA+TGG + chr8_2:47296420-47296439 Msa1251340:CDS 60.0%
AATTCCGCATCGTGCCCCGA+TGG + chr8_2:47296530-47296549 Msa1251340:CDS 60.0%
ATTCCGCATCGTGCCCCGAT+GGG + chr8_2:47296531-47296550 Msa1251340:CDS 60.0%
CAGCACTAGTCCTCCCATCG+GGG - chr8_2:47296547-47296566 None:intergenic 60.0%
GCAGCACTAGTCCTCCCATC+GGG - chr8_2:47296548-47296567 None:intergenic 60.0%
GAAAGGCCTCAGCCAAGGCC+CGG + chr8_2:47295600-47295619 Msa1251340:CDS 65.0%
! CCTTTTCCGGGCCTTGGCTG+AGG - chr8_2:47295609-47295628 None:intergenic 65.0%
CCTCAGCCAAGGCCCGGAAA+AGG + chr8_2:47295606-47295625 Msa1251340:CDS 65.0%
CCTCCCATCGGGGCACGATG+CGG - chr8_2:47296537-47296556 None:intergenic 70.0%
CCGCATCGTGCCCCGATGGG+AGG + chr8_2:47296534-47296553 Msa1251340:CDS 75.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 47293812 47297231 47293812 ID=Msa1251340;Name=Msa1251340
chr8_2 mRNA 47293812 47297231 47293812 ID=Msa1251340-mRNA-1;Parent=Msa1251340;Name=Msa1251340-mRNA-1;_AED=0.03;_eAED=0.03;_QI=245|1|1|1|0.75|0.6|5|626|235
chr8_2 exon 47293812 47294116 47293812 ID=Msa1251340-mRNA-1:exon:14317;Parent=Msa1251340-mRNA-1
chr8_2 exon 47294794 47294991 47294794 ID=Msa1251340-mRNA-1:exon:14318;Parent=Msa1251340-mRNA-1
chr8_2 exon 47295486 47295701 47295486 ID=Msa1251340-mRNA-1:exon:14319;Parent=Msa1251340-mRNA-1
chr8_2 exon 47296342 47297016 47296342 ID=Msa1251340-mRNA-1:exon:14320;Parent=Msa1251340-mRNA-1
chr8_2 exon 47297047 47297231 47297047 ID=Msa1251340-mRNA-1:exon:14321;Parent=Msa1251340-mRNA-1
chr8_2 five_prime_UTR 47293812 47294056 47293812 ID=Msa1251340-mRNA-1:five_prime_utr;Parent=Msa1251340-mRNA-1
chr8_2 CDS 47294057 47294116 47294057 ID=Msa1251340-mRNA-1:cds;Parent=Msa1251340-mRNA-1
chr8_2 CDS 47294794 47294991 47294794 ID=Msa1251340-mRNA-1:cds;Parent=Msa1251340-mRNA-1
chr8_2 CDS 47295486 47295701 47295486 ID=Msa1251340-mRNA-1:cds;Parent=Msa1251340-mRNA-1
chr8_2 CDS 47296342 47296575 47296342 ID=Msa1251340-mRNA-1:cds;Parent=Msa1251340-mRNA-1
chr8_2 three_prime_UTR 47296576 47297016 47296576 ID=Msa1251340-mRNA-1:three_prime_utr;Parent=Msa1251340-mRNA-1
chr8_2 three_prime_UTR 47297047 47297231 47297047 ID=Msa1251340-mRNA-1:three_prime_utr;Parent=Msa1251340-mRNA-1
Gene Sequence

>Msa1251340

ATGGCGTTTGAAAATCAAGACACTGTCCGTGAAATTAAGCCCAAAAACAGGAGAATCATGGGTGCTGGAGGTCCTGATGATGAGGAAAACAGGTGGCCACCATGGTTGAAACCTCTGCTCAAAGAGCGTTTCTTTGTTCAATGCAAGTTGCATGCTGATTCACACAAGAGTGAGTGCAATATGTATTGTTTGGATTGTATGAATGGTGCTCTTTGCTCACTCTGTCTCAATTACCACAAAGATCATCGTGCTATTCAGATAAGAAGGTCTTCATACCATGATGTGATTAGGGTGAATGAGATTCAGAAAGTGTTGGACATAACAGGTGTTCAAACCTATATTATCAACAGTGCAAGGGTTGTGTTCTTGAATGAAAGGCCTCAGCCAAGGCCCGGAAAAGGTGTCACCAACACTTGTGAAGTCTGCGAGCGTAGCCTTCTCGATTCCTTTCGATTCTGTTCTCTTGGCTGCAAGATTGTTGGAACTTCAATAAATTTCCAGAAGAAGAACAAGTCAGCTCGTGCTTCAATGATATCAGACTCAGAGGATTCACACAGCAGCAGTTGCAGCCATGGAAGACTGAAGAACATTAAGGTGCAAAGTTTCACTCCTTCAACACCACCACCAACAGTCAATTACAGATCAGCCAAAAGAAGAAAGGGAATTCCGCATCGTGCCCCGATGGGAGGACTAGTGCTGCAATACTAA
Protein sequence

>Msa1251340

MAFENQDTVREIKPKNRRIMGAGGPDDEENRWPPWLKPLLKERFFVQCKLHADSHKSECNMYCLDCMNGALCSLCLNYHKDHRAIQIRRSSYHDVIRVNEIQKVLDITGVQTYIINSARVVFLNERPQPRPGKGVTNTCEVCERSLLDSFRFCSLGCKIVGTSINFQKKNKSARASMISDSEDSHSSSCSHGRLKNIKVQSFTPSTPPPTVNYRSAKRRKGIPHRAPMGGLVLQY*