AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1256920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1256920 MtrunA17_Chr4g0055191 97.452 157 4 0 1 157 1 157 2.56e-115 323
Msa1256920 MtrunA17_Chr5g0400051 60.588 170 44 10 3 157 4 165 3.64e-56 173
Msa1256920 MtrunA17_Chr8g0336591 60.976 82 25 2 61 142 32 106 2.14e-29 103
Msa1256920 MtrunA17_Chr3g0124361 61.702 47 18 0 71 117 122 168 8.39e-15 68.6
Msa1256920 MtrunA17_Chr8g0344991 63.636 44 16 0 73 116 39 82 1.58e-14 65.9
Msa1256920 MtrunA17_Chr3g0115551 46.269 67 35 1 51 117 69 134 6.24e-13 62.8
Msa1256920 MtrunA17_Chr2g0295651 46.552 58 31 0 62 119 53 110 1.78e-12 61.2
Msa1256920 MtrunA17_Chr4g0070221 42.105 76 43 1 42 117 147 221 1.95e-11 60.1
Msa1256920 MtrunA17_Chr2g0301141 64.444 45 15 1 73 117 186 229 2.02e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1256920 AT4G17670.1 48.171 164 73 4 1 157 1 159 5.77e-38 127
Msa1256920 AT5G47060.2 62.000 100 32 2 59 157 83 177 9.36e-38 127
Msa1256920 AT5G47060.1 62.000 100 32 2 59 157 83 177 9.36e-38 127
Msa1256920 AT2G44670.1 47.423 97 44 1 61 157 4 93 1.21e-23 89.0
Msa1256920 AT1G22160.1 48.529 68 33 1 51 118 58 123 8.23e-15 67.8
Msa1256920 AT1G78020.1 43.333 90 36 3 38 118 51 134 9.31e-15 68.2
Msa1256920 AT5G65040.1 50.877 57 27 1 73 129 57 112 8.13e-14 64.3
Msa1256920 AT1G53885.1 43.243 74 38 1 73 146 42 111 3.53e-13 63.2
Msa1256920 AT1G53903.1 43.243 74 38 1 73 146 42 111 3.53e-13 63.2
Msa1256920 AT4G39795.1 46.296 54 29 0 73 126 73 126 9.34e-11 56.6

Find 51 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTGTAAATGGTTTACTTT+TGG 0.244663 8_2:+57229314 Msa1256920:three_prime_UTR
TATGAAAGCTTTGAGGAATA+AGG 0.290096 8_2:+57229055 Msa1256920:CDS
TATTAAAATTGAAGGGGTTT+TGG 0.291458 8_2:-57228535 None:intergenic
AAAAGTGTGTTTCCTTGTTT+TGG 0.307447 8_2:+57229337 Msa1256920:three_prime_UTR
GCAGAGAATGTAACTGTAAA+TGG 0.317276 8_2:+57229303 Msa1256920:three_prime_UTR
GGTTATGTTGTGCTCATATA+TGG 0.334681 8_2:+57229470 Msa1256920:three_prime_UTR
AAGATTATTCCTTTCGTAAA+GGG 0.361565 8_2:+57229117 Msa1256920:CDS
CTACACTCTTCACTACAAAA+AGG 0.366837 8_2:-57228975 None:intergenic
GAGAACAAGCTTCAAGGAAG+TGG 0.366962 8_2:-57228775 None:intergenic
AATAGAGATAGATGAATTGA+AGG 0.378058 8_2:+57229007 Msa1256920:CDS
CCTCAATCTATCTATAGAAA+TGG 0.387484 8_2:+57229548 Msa1256920:three_prime_UTR
CTTCATCTATGAAAGCTTTG+AGG 0.390443 8_2:+57229048 Msa1256920:CDS
TGCAACATCCATGTCTTTCA+AGG 0.400148 8_2:-57228615 None:intergenic
CATGAGTTTATTAAAATTGA+AGG 0.407097 8_2:-57228543 None:intergenic
ATAAAGATATCTTCATGTAT+AGG 0.420008 8_2:+57228822 Msa1256920:CDS
TCATAGAATCTTCCAGATCT+AGG 0.432287 8_2:-57228728 None:intergenic
TATGTTTCTTCACCTAGATC+TGG 0.436471 8_2:+57228716 Msa1256920:CDS
CAAGATTATTCCTTTCGTAA+AGG 0.446366 8_2:+57229116 Msa1256920:CDS
TACAAAGAGAACAAGCTTCA+AGG 0.448678 8_2:-57228781 None:intergenic
AAACTGTGTCATTTCATTGA+AGG 0.449010 8_2:-57229366 None:intergenic
ATCTAGGTGAAGAAACATAA+AGG 0.450130 8_2:-57228712 None:intergenic
AATTCTTCAAACAAGGTTCA+AGG 0.457717 8_2:+57229092 Msa1256920:CDS
AACTGTGTCATTTCATTGAA+GGG 0.479606 8_2:-57229365 None:intergenic
ATGAGTTTATTAAAATTGAA+GGG 0.500742 8_2:-57228542 None:intergenic
CACACCATATAGATTACAAA+AGG 0.502625 8_2:-57229448 None:intergenic
CCATTTCTATAGATAGATTG+AGG 0.513071 8_2:-57229548 None:intergenic
CTTTGTAACAAACCACTTGG+TGG 0.515974 8_2:+57228797 Msa1256920:CDS
AAATTTGTTGAGAGAGTGAA+GGG 0.521559 8_2:-57228495 None:intergenic
AGAACAAGCTTCAAGGAAGT+GGG 0.529651 8_2:-57228774 None:intergenic
GAAATTTGTTGAGAGAGTGA+AGG 0.537589 8_2:-57228496 None:intergenic
TACAAAATCTATGATAACCA+TGG 0.556899 8_2:+57228661 Msa1256920:CDS
CGAACAAGAGTCAATAGAGA+AGG 0.565185 8_2:+57229273 Msa1256920:three_prime_UTR
GGTCACACTGTTAGATTGCA+CGG 0.571251 8_2:-57229574 None:intergenic
TTTAGCTTCCTTGAAAGACA+TGG 0.578512 8_2:+57228607 Msa1256920:CDS
TGAGTTTATTAAAATTGAAG+GGG 0.585248 8_2:-57228541 None:intergenic
ACAAAATCTATGATAACCAT+GGG 0.587457 8_2:+57228662 Msa1256920:CDS
AAAATCTAATTCTTCAAACA+AGG 0.600129 8_2:+57229085 Msa1256920:CDS
ATATAGATTACAAAAGGAGG+AGG 0.602148 8_2:-57229442 None:intergenic
AGTATTATTGACATAACCCA+TGG 0.603157 8_2:-57228678 None:intergenic
TGAAGGGATCTTCCAAAACA+AGG 0.605895 8_2:-57229349 None:intergenic
TGGTCTTGTTCTAAGTAACA+AGG 0.607552 8_2:-57228581 None:intergenic
TCTCTTTGTAACAAACCACT+TGG 0.609904 8_2:+57228794 Msa1256920:CDS
ACCATATAGATTACAAAAGG+AGG 0.613025 8_2:-57229445 None:intergenic
ATGGATGTTGCAAGTTATCG+TGG 0.615490 8_2:+57228626 Msa1256920:CDS
AATAGCTGTCCCTTTACGAA+AGG 0.633199 8_2:-57229126 None:intergenic
ATATCTTTATTGCCACCAAG+TGG 0.645406 8_2:-57228809 None:intergenic
GAACAAGAGTCAATAGAGAA+GGG 0.648837 8_2:+57229274 Msa1256920:three_prime_UTR
AGATGGAACTGAATATACAA+TGG 0.668750 8_2:+57229507 Msa1256920:three_prime_UTR
GAACAAGCTTCAAGGAAGTG+GGG 0.688186 8_2:-57228773 None:intergenic
TGTTACTTAGAACAAGACCA+CGG 0.706661 8_2:+57228584 Msa1256920:CDS
CTTTCAAGGAAGCTAAACCG+TGG 0.750905 8_2:-57228601 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTCAATTAAAAAAACAT+GGG - chr8_2:57228852-57228871 None:intergenic 10.0%
!! ATATTTCAATTAAAAAAACA+TGG - chr8_2:57228853-57228872 None:intergenic 10.0%
!! TATTTCAATTAAAAAAACAT+GGG - chr8_2:57228852-57228871 None:intergenic 10.0%
!! ATATTTCAATTAAAAAAACA+TGG - chr8_2:57228853-57228872 None:intergenic 10.0%
!! ATGAGTTTATTAAAATTGAA+GGG - chr8_2:57228545-57228564 None:intergenic 15.0%
!!! CATGAAAATTTTCATAATAA+GGG - chr8_2:57228891-57228910 None:intergenic 15.0%
!!! TCATGAAAATTTTCATAATA+AGG - chr8_2:57228892-57228911 None:intergenic 15.0%
!! ATGAGTTTATTAAAATTGAA+GGG - chr8_2:57228545-57228564 None:intergenic 15.0%
!!! CATGAAAATTTTCATAATAA+GGG - chr8_2:57228891-57228910 None:intergenic 15.0%
!!! TCATGAAAATTTTCATAATA+AGG - chr8_2:57228892-57228911 None:intergenic 15.0%
!! TGAGTTTATTAAAATTGAAG+GGG - chr8_2:57228544-57228563 None:intergenic 20.0%
!! CATGAGTTTATTAAAATTGA+AGG - chr8_2:57228546-57228565 None:intergenic 20.0%
!!! CTTCAATTTTAATAAACTCA+TGG + chr8_2:57228544-57228563 Msa1256920:exon 20.0%
!! ATAAAGATATCTTCATGTAT+AGG + chr8_2:57228822-57228841 Msa1256920:CDS 20.0%
!!! TTCATAATAAGGGTTTTTTA+AGG - chr8_2:57228881-57228900 None:intergenic 20.0%
!!! ATGATCGATGTTTTTTTTTA+AGG + chr8_2:57228908-57228927 Msa1256920:intron 20.0%
!! AAAATCTAATTCTTCAAACA+AGG + chr8_2:57229085-57229104 Msa1256920:CDS 20.0%
!! TGAGTTTATTAAAATTGAAG+GGG - chr8_2:57228544-57228563 None:intergenic 20.0%
!! CATGAGTTTATTAAAATTGA+AGG - chr8_2:57228546-57228565 None:intergenic 20.0%
!!! CTTCAATTTTAATAAACTCA+TGG + chr8_2:57228544-57228563 Msa1256920:exon 20.0%
!! ATAAAGATATCTTCATGTAT+AGG + chr8_2:57228822-57228841 Msa1256920:CDS 20.0%
!!! TTCATAATAAGGGTTTTTTA+AGG - chr8_2:57228881-57228900 None:intergenic 20.0%
!!! ATGATCGATGTTTTTTTTTA+AGG + chr8_2:57228908-57228927 Msa1256920:intron 20.0%
!! AAAATCTAATTCTTCAAACA+AGG + chr8_2:57229085-57229104 Msa1256920:CDS 20.0%
!! TATTAAAATTGAAGGGGTTT+TGG - chr8_2:57228538-57228557 None:intergenic 25.0%
! TACAAAATCTATGATAACCA+TGG + chr8_2:57228661-57228680 Msa1256920:CDS 25.0%
! ACAAAATCTATGATAACCAT+GGG + chr8_2:57228662-57228681 Msa1256920:CDS 25.0%
!!! TGATTGATTTTAATTTGTGC+AGG + chr8_2:57228943-57228962 Msa1256920:intron 25.0%
!!! GATTGATTTTAATTTGTGCA+GGG + chr8_2:57228944-57228963 Msa1256920:intron 25.0%
!!! ATTGATTTTAATTTGTGCAG+GGG + chr8_2:57228945-57228964 Msa1256920:intron 25.0%
! AATAGAGATAGATGAATTGA+AGG + chr8_2:57229007-57229026 Msa1256920:CDS 25.0%
! AAGATTATTCCTTTCGTAAA+GGG + chr8_2:57229117-57229136 Msa1256920:CDS 25.0%
!! AACTGTAAATGGTTTACTTT+TGG + chr8_2:57229314-57229333 Msa1256920:three_prime_UTR 25.0%
!! AATGAAATGACACAGTTTTT+GGG + chr8_2:57229371-57229390 Msa1256920:three_prime_UTR 25.0%
!!! CTGTTTTTTTTTTGAGATCA+AGG + chr8_2:57229409-57229428 Msa1256920:three_prime_UTR 25.0%
!!! TTTTTTTGAGATCAAGGTTT+TGG + chr8_2:57229415-57229434 Msa1256920:three_prime_UTR 25.0%
!! TATTAAAATTGAAGGGGTTT+TGG - chr8_2:57228538-57228557 None:intergenic 25.0%
! TACAAAATCTATGATAACCA+TGG + chr8_2:57228661-57228680 Msa1256920:CDS 25.0%
! ACAAAATCTATGATAACCAT+GGG + chr8_2:57228662-57228681 Msa1256920:CDS 25.0%
!!! TGATTGATTTTAATTTGTGC+AGG + chr8_2:57228943-57228962 Msa1256920:intron 25.0%
!!! GATTGATTTTAATTTGTGCA+GGG + chr8_2:57228944-57228963 Msa1256920:intron 25.0%
!!! ATTGATTTTAATTTGTGCAG+GGG + chr8_2:57228945-57228964 Msa1256920:intron 25.0%
! AATAGAGATAGATGAATTGA+AGG + chr8_2:57229007-57229026 Msa1256920:CDS 25.0%
! AAGATTATTCCTTTCGTAAA+GGG + chr8_2:57229117-57229136 Msa1256920:CDS 25.0%
!! AACTGTAAATGGTTTACTTT+TGG + chr8_2:57229314-57229333 Msa1256920:three_prime_UTR 25.0%
!! AATGAAATGACACAGTTTTT+GGG + chr8_2:57229371-57229390 Msa1256920:three_prime_UTR 25.0%
!!! CTGTTTTTTTTTTGAGATCA+AGG + chr8_2:57229409-57229428 Msa1256920:three_prime_UTR 25.0%
!!! TTTTTTTGAGATCAAGGTTT+TGG + chr8_2:57229415-57229434 Msa1256920:three_prime_UTR 25.0%
AGTATTATTGACATAACCCA+TGG - chr8_2:57228681-57228700 None:intergenic 30.0%
ATCTAGGTGAAGAAACATAA+AGG - chr8_2:57228715-57228734 None:intergenic 30.0%
!! TATGAAAGCTTTGAGGAATA+AGG + chr8_2:57229055-57229074 Msa1256920:CDS 30.0%
AATTCTTCAAACAAGGTTCA+AGG + chr8_2:57229092-57229111 Msa1256920:CDS 30.0%
CAAGATTATTCCTTTCGTAA+AGG + chr8_2:57229116-57229135 Msa1256920:CDS 30.0%
AAAACCGTGACAGAAAAAAA+AGG + chr8_2:57229196-57229215 Msa1256920:three_prime_UTR 30.0%
AAACCGTGACAGAAAAAAAA+GGG + chr8_2:57229197-57229216 Msa1256920:three_prime_UTR 30.0%
!!! TTTCCCTTTTTTTTCTGTCA+CGG - chr8_2:57229203-57229222 None:intergenic 30.0%
!! AAAAGTGTGTTTCCTTGTTT+TGG + chr8_2:57229337-57229356 Msa1256920:three_prime_UTR 30.0%
!! AACTGTGTCATTTCATTGAA+GGG - chr8_2:57229368-57229387 None:intergenic 30.0%
!! AAACTGTGTCATTTCATTGA+AGG - chr8_2:57229369-57229388 None:intergenic 30.0%
! CAATGAAATGACACAGTTTT+TGG + chr8_2:57229370-57229389 Msa1256920:three_prime_UTR 30.0%
ATATAGATTACAAAAGGAGG+AGG - chr8_2:57229445-57229464 None:intergenic 30.0%
! TCCTCCTTTTGTAATCTATA+TGG + chr8_2:57229444-57229463 Msa1256920:three_prime_UTR 30.0%
ACCATATAGATTACAAAAGG+AGG - chr8_2:57229448-57229467 None:intergenic 30.0%
CACACCATATAGATTACAAA+AGG - chr8_2:57229451-57229470 None:intergenic 30.0%
! CTTTTGTAATCTATATGGTG+TGG + chr8_2:57229449-57229468 Msa1256920:three_prime_UTR 30.0%
!! TGGTTTCAGTTTTTCAAAGA+TGG + chr8_2:57229490-57229509 Msa1256920:three_prime_UTR 30.0%
AGATGGAACTGAATATACAA+TGG + chr8_2:57229507-57229526 Msa1256920:three_prime_UTR 30.0%
CCATTTCTATAGATAGATTG+AGG - chr8_2:57229551-57229570 None:intergenic 30.0%
CCTCAATCTATCTATAGAAA+TGG + chr8_2:57229548-57229567 Msa1256920:three_prime_UTR 30.0%
AGTATTATTGACATAACCCA+TGG - chr8_2:57228681-57228700 None:intergenic 30.0%
ATCTAGGTGAAGAAACATAA+AGG - chr8_2:57228715-57228734 None:intergenic 30.0%
!! TATGAAAGCTTTGAGGAATA+AGG + chr8_2:57229055-57229074 Msa1256920:CDS 30.0%
AATTCTTCAAACAAGGTTCA+AGG + chr8_2:57229092-57229111 Msa1256920:CDS 30.0%
CAAGATTATTCCTTTCGTAA+AGG + chr8_2:57229116-57229135 Msa1256920:CDS 30.0%
AAAACCGTGACAGAAAAAAA+AGG + chr8_2:57229196-57229215 Msa1256920:three_prime_UTR 30.0%
AAACCGTGACAGAAAAAAAA+GGG + chr8_2:57229197-57229216 Msa1256920:three_prime_UTR 30.0%
!!! TTTCCCTTTTTTTTCTGTCA+CGG - chr8_2:57229203-57229222 None:intergenic 30.0%
!! AAAAGTGTGTTTCCTTGTTT+TGG + chr8_2:57229337-57229356 Msa1256920:three_prime_UTR 30.0%
!! AACTGTGTCATTTCATTGAA+GGG - chr8_2:57229368-57229387 None:intergenic 30.0%
!! AAACTGTGTCATTTCATTGA+AGG - chr8_2:57229369-57229388 None:intergenic 30.0%
! CAATGAAATGACACAGTTTT+TGG + chr8_2:57229370-57229389 Msa1256920:three_prime_UTR 30.0%
ATATAGATTACAAAAGGAGG+AGG - chr8_2:57229445-57229464 None:intergenic 30.0%
! TCCTCCTTTTGTAATCTATA+TGG + chr8_2:57229444-57229463 Msa1256920:three_prime_UTR 30.0%
ACCATATAGATTACAAAAGG+AGG - chr8_2:57229448-57229467 None:intergenic 30.0%
CACACCATATAGATTACAAA+AGG - chr8_2:57229451-57229470 None:intergenic 30.0%
! CTTTTGTAATCTATATGGTG+TGG + chr8_2:57229449-57229468 Msa1256920:three_prime_UTR 30.0%
!! TGGTTTCAGTTTTTCAAAGA+TGG + chr8_2:57229490-57229509 Msa1256920:three_prime_UTR 30.0%
AGATGGAACTGAATATACAA+TGG + chr8_2:57229507-57229526 Msa1256920:three_prime_UTR 30.0%
CCATTTCTATAGATAGATTG+AGG - chr8_2:57229551-57229570 None:intergenic 30.0%
CCTCAATCTATCTATAGAAA+TGG + chr8_2:57229548-57229567 Msa1256920:three_prime_UTR 30.0%
!!! AATTGAAGGGGTTTTGGTTT+TGG - chr8_2:57228532-57228551 None:intergenic 35.0%
!! TGGTCTTGTTCTAAGTAACA+AGG - chr8_2:57228584-57228603 None:intergenic 35.0%
! TGTTACTTAGAACAAGACCA+CGG + chr8_2:57228584-57228603 Msa1256920:CDS 35.0%
! TTTAGCTTCCTTGAAAGACA+TGG + chr8_2:57228607-57228626 Msa1256920:CDS 35.0%
TATGTTTCTTCACCTAGATC+TGG + chr8_2:57228716-57228735 Msa1256920:CDS 35.0%
TCATAGAATCTTCCAGATCT+AGG - chr8_2:57228731-57228750 None:intergenic 35.0%
TACAAAGAGAACAAGCTTCA+AGG - chr8_2:57228784-57228803 None:intergenic 35.0%
TCTCTTTGTAACAAACCACT+TGG + chr8_2:57228794-57228813 Msa1256920:CDS 35.0%
ATATCTTTATTGCCACCAAG+TGG - chr8_2:57228812-57228831 None:intergenic 35.0%
CTACACTCTTCACTACAAAA+AGG - chr8_2:57228978-57228997 None:intergenic 35.0%
!! CTTTTTGTAGTGAAGAGTGT+AGG + chr8_2:57228976-57228995 Msa1256920:CDS 35.0%
CTTCATCTATGAAAGCTTTG+AGG + chr8_2:57229048-57229067 Msa1256920:CDS 35.0%
GAACAAGAGTCAATAGAGAA+GGG + chr8_2:57229274-57229293 Msa1256920:three_prime_UTR 35.0%
GCAGAGAATGTAACTGTAAA+TGG + chr8_2:57229303-57229322 Msa1256920:three_prime_UTR 35.0%
!!! AATGACACAGTTTTTGGGTT+TGG + chr8_2:57229376-57229395 Msa1256920:three_prime_UTR 35.0%
!!! ATGACACAGTTTTTGGGTTT+GGG + chr8_2:57229377-57229396 Msa1256920:three_prime_UTR 35.0%
GGTTATGTTGTGCTCATATA+TGG + chr8_2:57229470-57229489 Msa1256920:three_prime_UTR 35.0%
!! GAAATTTGTTGAGAGAGTGA+AGG - chr8_2:57228499-57228518 None:intergenic 35.0%
!!! AATTGAAGGGGTTTTGGTTT+TGG - chr8_2:57228532-57228551 None:intergenic 35.0%
!! TGGTCTTGTTCTAAGTAACA+AGG - chr8_2:57228584-57228603 None:intergenic 35.0%
! TGTTACTTAGAACAAGACCA+CGG + chr8_2:57228584-57228603 Msa1256920:CDS 35.0%
! TTTAGCTTCCTTGAAAGACA+TGG + chr8_2:57228607-57228626 Msa1256920:CDS 35.0%
TATGTTTCTTCACCTAGATC+TGG + chr8_2:57228716-57228735 Msa1256920:CDS 35.0%
TCATAGAATCTTCCAGATCT+AGG - chr8_2:57228731-57228750 None:intergenic 35.0%
TACAAAGAGAACAAGCTTCA+AGG - chr8_2:57228784-57228803 None:intergenic 35.0%
TCTCTTTGTAACAAACCACT+TGG + chr8_2:57228794-57228813 Msa1256920:CDS 35.0%
ATATCTTTATTGCCACCAAG+TGG - chr8_2:57228812-57228831 None:intergenic 35.0%
CTACACTCTTCACTACAAAA+AGG - chr8_2:57228978-57228997 None:intergenic 35.0%
!! CTTTTTGTAGTGAAGAGTGT+AGG + chr8_2:57228976-57228995 Msa1256920:CDS 35.0%
CTTCATCTATGAAAGCTTTG+AGG + chr8_2:57229048-57229067 Msa1256920:CDS 35.0%
GAACAAGAGTCAATAGAGAA+GGG + chr8_2:57229274-57229293 Msa1256920:three_prime_UTR 35.0%
GCAGAGAATGTAACTGTAAA+TGG + chr8_2:57229303-57229322 Msa1256920:three_prime_UTR 35.0%
!!! AATGACACAGTTTTTGGGTT+TGG + chr8_2:57229376-57229395 Msa1256920:three_prime_UTR 35.0%
!!! ATGACACAGTTTTTGGGTTT+GGG + chr8_2:57229377-57229396 Msa1256920:three_prime_UTR 35.0%
GGTTATGTTGTGCTCATATA+TGG + chr8_2:57229470-57229489 Msa1256920:three_prime_UTR 35.0%
TGCAACATCCATGTCTTTCA+AGG - chr8_2:57228618-57228637 None:intergenic 40.0%
ATGGATGTTGCAAGTTATCG+TGG + chr8_2:57228626-57228645 Msa1256920:CDS 40.0%
AGAACAAGCTTCAAGGAAGT+GGG - chr8_2:57228777-57228796 None:intergenic 40.0%
CTTTGTAACAAACCACTTGG+TGG + chr8_2:57228797-57228816 Msa1256920:CDS 40.0%
AATAGCTGTCCCTTTACGAA+AGG - chr8_2:57229129-57229148 None:intergenic 40.0%
!! TAGTTTTGCTTGAGCGGAAA+AGG + chr8_2:57229155-57229174 Msa1256920:exon 40.0%
CGAACAAGAGTCAATAGAGA+AGG + chr8_2:57229273-57229292 Msa1256920:three_prime_UTR 40.0%
TGAAGGGATCTTCCAAAACA+AGG - chr8_2:57229352-57229371 None:intergenic 40.0%
!!! ACAGTTTTTGGGTTTGGGTT+TGG + chr8_2:57229382-57229401 Msa1256920:three_prime_UTR 40.0%
!!! CAGTTTTTGGGTTTGGGTTT+GGG + chr8_2:57229383-57229402 Msa1256920:three_prime_UTR 40.0%
TGCAACATCCATGTCTTTCA+AGG - chr8_2:57228618-57228637 None:intergenic 40.0%
ATGGATGTTGCAAGTTATCG+TGG + chr8_2:57228626-57228645 Msa1256920:CDS 40.0%
AGAACAAGCTTCAAGGAAGT+GGG - chr8_2:57228777-57228796 None:intergenic 40.0%
CTTTGTAACAAACCACTTGG+TGG + chr8_2:57228797-57228816 Msa1256920:CDS 40.0%
AATAGCTGTCCCTTTACGAA+AGG - chr8_2:57229129-57229148 None:intergenic 40.0%
!! TAGTTTTGCTTGAGCGGAAA+AGG + chr8_2:57229155-57229174 Msa1256920:exon 40.0%
CGAACAAGAGTCAATAGAGA+AGG + chr8_2:57229273-57229292 Msa1256920:three_prime_UTR 40.0%
TGAAGGGATCTTCCAAAACA+AGG - chr8_2:57229352-57229371 None:intergenic 40.0%
!!! ACAGTTTTTGGGTTTGGGTT+TGG + chr8_2:57229382-57229401 Msa1256920:three_prime_UTR 40.0%
!!! CAGTTTTTGGGTTTGGGTTT+GGG + chr8_2:57229383-57229402 Msa1256920:three_prime_UTR 40.0%
!!! AGGGGTTTTGGTTTTGGTTG+AGG - chr8_2:57228526-57228545 None:intergenic 45.0%
CTTTCAAGGAAGCTAAACCG+TGG - chr8_2:57228604-57228623 None:intergenic 45.0%
GAACAAGCTTCAAGGAAGTG+GGG - chr8_2:57228776-57228795 None:intergenic 45.0%
GAGAACAAGCTTCAAGGAAG+TGG - chr8_2:57228778-57228797 None:intergenic 45.0%
!! GCAGCATAGTTTTGCTTGAG+CGG + chr8_2:57229149-57229168 Msa1256920:exon 45.0%
!!! AGGGGTTTTGGTTTTGGTTG+AGG - chr8_2:57228526-57228545 None:intergenic 45.0%
CTTTCAAGGAAGCTAAACCG+TGG - chr8_2:57228604-57228623 None:intergenic 45.0%
GAACAAGCTTCAAGGAAGTG+GGG - chr8_2:57228776-57228795 None:intergenic 45.0%
GAGAACAAGCTTCAAGGAAG+TGG - chr8_2:57228778-57228797 None:intergenic 45.0%
!! GCAGCATAGTTTTGCTTGAG+CGG + chr8_2:57229149-57229168 Msa1256920:exon 45.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 57228497 57229587 57228497 ID=Msa1256920;Name=Msa1256920
chr8_2 mRNA 57228497 57229587 57228497 ID=Msa1256920-mRNA-1;Parent=Msa1256920;Name=Msa1256920-mRNA-1;_AED=0.01;_eAED=0.01;_QI=66|1|1|1|1|1|2|430|157
chr8_2 exon 57228497 57228843 57228497 ID=Msa1256920-mRNA-1:exon:17643;Parent=Msa1256920-mRNA-1
chr8_2 exon 57228965 57229587 57228965 ID=Msa1256920-mRNA-1:exon:17644;Parent=Msa1256920-mRNA-1
chr8_2 five_prime_UTR 57228497 57228562 57228497 ID=Msa1256920-mRNA-1:five_prime_utr;Parent=Msa1256920-mRNA-1
chr8_2 CDS 57228563 57228843 57228563 ID=Msa1256920-mRNA-1:cds;Parent=Msa1256920-mRNA-1
chr8_2 CDS 57228965 57229157 57228965 ID=Msa1256920-mRNA-1:cds;Parent=Msa1256920-mRNA-1
chr8_2 three_prime_UTR 57229158 57229587 57229158 ID=Msa1256920-mRNA-1:three_prime_utr;Parent=Msa1256920-mRNA-1
Gene Sequence

>Msa1256920

ATGGACTCATCAATAAAACCTTGTTACTTAGAACAAGACCACGGTTTAGCTTCCTTGAAAGACATGGATGTTGCAAGTTATCGTGGCAATAGTTTTGTTACAAAATCTATGATAACCATGGGTTATGTCAATAATACTAGTTTCAAGAACCTTTATGTTTCTTCACCTAGATCTGGAAGATTCTATGATGCTAGATTTGAAGATCATCAACCCCACTTCCTTGAAGCTTGTTCTCTTTGTAACAAACCACTTGGTGGCAATAAAGATATCTTCATGTATAGGGGTGACACACCTTTTTGTAGTGAAGAGTGTAGGCAAGAACAAATAGAGATAGATGAATTGAAGGAGAAGAATATGAATCTTTCTTCATCTATGAAAGCTTTGAGGAATAAGGAACATAGAAAATCTAATTCTTCAAACAAGGTTCAAGGACAAGATTATTCCTTTCGTAAAGGGACAGCTATTGCAGCATAG
Protein sequence

>Msa1256920

MDSSIKPCYLEQDHGLASLKDMDVASYRGNSFVTKSMITMGYVNNTSFKNLYVSSPRSGRFYDARFEDHQPHFLEACSLCNKPLGGNKDIFMYRGDTPFCSEECRQEQIEIDELKEKNMNLSSSMKALRNKEHRKSNSSNKVQGQDYSFRKGTAIAA*