AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1330810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1330810 MtrunA17_Chr4g0055191 97.452 157 4 0 1 157 1 157 2.56e-115 323
Msa1330810 MtrunA17_Chr5g0400051 60.588 170 44 10 3 157 4 165 3.64e-56 173
Msa1330810 MtrunA17_Chr8g0336591 60.976 82 25 2 61 142 32 106 2.14e-29 103
Msa1330810 MtrunA17_Chr3g0124361 61.702 47 18 0 71 117 122 168 8.39e-15 68.6
Msa1330810 MtrunA17_Chr8g0344991 63.636 44 16 0 73 116 39 82 1.58e-14 65.9
Msa1330810 MtrunA17_Chr3g0115551 46.269 67 35 1 51 117 69 134 6.24e-13 62.8
Msa1330810 MtrunA17_Chr2g0295651 46.552 58 31 0 62 119 53 110 1.78e-12 61.2
Msa1330810 MtrunA17_Chr4g0070221 42.105 76 43 1 42 117 147 221 1.95e-11 60.1
Msa1330810 MtrunA17_Chr2g0301141 64.444 45 15 1 73 117 186 229 2.02e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1330810 AT4G17670.1 48.171 164 73 4 1 157 1 159 5.77e-38 127
Msa1330810 AT5G47060.2 62.000 100 32 2 59 157 83 177 9.36e-38 127
Msa1330810 AT5G47060.1 62.000 100 32 2 59 157 83 177 9.36e-38 127
Msa1330810 AT2G44670.1 47.423 97 44 1 61 157 4 93 1.21e-23 89.0
Msa1330810 AT1G22160.1 48.529 68 33 1 51 118 58 123 8.23e-15 67.8
Msa1330810 AT1G78020.1 43.333 90 36 3 38 118 51 134 9.31e-15 68.2
Msa1330810 AT5G65040.1 50.877 57 27 1 73 129 57 112 8.13e-14 64.3
Msa1330810 AT1G53885.1 43.243 74 38 1 73 146 42 111 3.53e-13 63.2
Msa1330810 AT1G53903.1 43.243 74 38 1 73 146 42 111 3.53e-13 63.2
Msa1330810 AT4G39795.1 46.296 54 29 0 73 126 73 126 9.34e-11 56.6

Find 48 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTGTAAATGGTTTACTTT+TGG 0.244663 8_4:+50044312 Msa1330810:three_prime_UTR
TATTAAAATTGAAGGGGTTT+TGG 0.291458 8_4:-50043522 None:intergenic
AAAAGTGTGTTTCCTTGTTT+TGG 0.307447 8_4:+50044335 Msa1330810:three_prime_UTR
GCAGAGAATGTAACTGTAAA+TGG 0.317276 8_4:+50044301 Msa1330810:three_prime_UTR
TATGAAGGCTTTGAGGAATA+AGG 0.349320 8_4:+50044047 Msa1330810:CDS
GTGGTTGTGTTGTGCTTATA+TGG 0.357268 8_4:+50044460 Msa1330810:three_prime_UTR
CTACACTCTTCACTACAAAA+AGG 0.366837 8_4:-50043967 None:intergenic
GAGAACAAGCTTCAAGGAAG+TGG 0.366962 8_4:-50043762 None:intergenic
CCTCAATCTATCTATAGAAA+TGG 0.387484 8_4:+50044538 Msa1330810:three_prime_UTR
TGCAACATCCATGTCTTTCA+AGG 0.400148 8_4:-50043602 None:intergenic
CATGAGTTTATTAAAATTGA+AGG 0.407097 8_4:-50043530 None:intergenic
GAATCTTTCTTCATCTATGA+AGG 0.411030 8_4:+50044032 Msa1330810:CDS
CTTCATCTATGAAGGCTTTG+AGG 0.411788 8_4:+50044040 Msa1330810:CDS
ATAAAGATATCTTCATGTAT+AGG 0.420008 8_4:+50043809 Msa1330810:CDS
TCATAGAATCTTCCAGATCT+AGG 0.432287 8_4:-50043715 None:intergenic
TATGTTTCTTCACCTAGATC+TGG 0.436471 8_4:+50043703 Msa1330810:CDS
TACAAAGAGAACAAGCTTCA+AGG 0.448678 8_4:-50043768 None:intergenic
ATCTAGGTGAAGAAACATAA+AGG 0.450130 8_4:-50043699 None:intergenic
AAACTGAGTCATTTCATTGA+AGG 0.455100 8_4:-50044364 None:intergenic
AATTCTTCAAACAAGGTTCA+AGG 0.457717 8_4:+50044084 Msa1330810:CDS
CAAGATTATTCCTTTCGTAA+AGG 0.463468 8_4:+50044108 Msa1330810:CDS
AACTGAGTCATTTCATTGAA+GGG 0.494363 8_4:-50044363 None:intergenic
ATGAGTTTATTAAAATTGAA+GGG 0.500742 8_4:-50043529 None:intergenic
CACGCCATATAAATTACAAA+AGG 0.509155 8_4:-50044440 None:intergenic
CCATTTCTATAGATAGATTG+AGG 0.513071 8_4:-50044538 None:intergenic
CTTTGTAACAAACCACTTGG+TGG 0.515974 8_4:+50043784 Msa1330810:CDS
AGAACAAGCTTCAAGGAAGT+GGG 0.529651 8_4:-50043761 None:intergenic
TACAAAATCTATGATAACCA+TGG 0.556899 8_4:+50043648 Msa1330810:CDS
CGAACAAGAGTCAATAGAGA+AGG 0.565185 8_4:+50044271 Msa1330810:three_prime_UTR
GCCATATAAATTACAAAAGG+AGG 0.569172 8_4:-50044437 None:intergenic
GGTCACACTGTTAGATTGCA+CGG 0.571251 8_4:-50044564 None:intergenic
TTTAGCTTCCTTGAAAGACA+TGG 0.578512 8_4:+50043594 Msa1330810:CDS
TGAGTTTATTAAAATTGAAG+GGG 0.585248 8_4:-50043528 None:intergenic
ACAAAATCTATGATAACCAT+GGG 0.587457 8_4:+50043649 Msa1330810:CDS
AATAGCTGTTCCTTTACGAA+AGG 0.588257 8_4:-50044118 None:intergenic
ATATAAATTACAAAAGGAGG+AGG 0.595330 8_4:-50044434 None:intergenic
AAAATCTAATTCTTCAAACA+AGG 0.600129 8_4:+50044077 Msa1330810:CDS
AGTATTATTGACATAACCCA+TGG 0.603157 8_4:-50043665 None:intergenic
TGAAGGGATCTTCCAAAACA+AGG 0.605895 8_4:-50044347 None:intergenic
TGGTCTTGTTCTAAGTAACA+AGG 0.607552 8_4:-50043568 None:intergenic
TCTCTTTGTAACAAACCACT+TGG 0.609904 8_4:+50043781 Msa1330810:CDS
ATGGATGTTGCAAGTTATCG+TGG 0.615490 8_4:+50043613 Msa1330810:CDS
ATATCTTTATTGCCACCAAG+TGG 0.645406 8_4:-50043796 None:intergenic
GAACAAGAGTCAATAGAGAA+GGG 0.648837 8_4:+50044272 Msa1330810:three_prime_UTR
AGATGGAACTGAATATACAA+TGG 0.668750 8_4:+50044497 Msa1330810:three_prime_UTR
GAACAAGCTTCAAGGAAGTG+GGG 0.688186 8_4:-50043760 None:intergenic
TGTTACTTAGAACAAGACCA+CGG 0.706661 8_4:+50043571 Msa1330810:CDS
CTTTCAAGGAAGCTAAACCG+TGG 0.750905 8_4:-50043588 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTCAATTAAAAAAACAT+GGG - chr8_4:50043839-50043858 None:intergenic 10.0%
!! ATATTTCAATTAAAAAAACA+TGG - chr8_4:50043840-50043859 None:intergenic 10.0%
!!! TTTTTTTTTTTTTTTTGTCA+CGG - chr8_4:50044195-50044214 None:intergenic 10.0%
!! TGACAAAAAAAAAAAAAAAA+AGG + chr8_4:50044195-50044214 Msa1330810:three_prime_UTR 10.0%
!! TATTTCAATTAAAAAAACAT+GGG - chr8_4:50043839-50043858 None:intergenic 10.0%
!! ATATTTCAATTAAAAAAACA+TGG - chr8_4:50043840-50043859 None:intergenic 10.0%
!!! TTTTTTTTTTTTTTTTGTCA+CGG - chr8_4:50044195-50044214 None:intergenic 10.0%
!! TGACAAAAAAAAAAAAAAAA+AGG + chr8_4:50044195-50044214 Msa1330810:three_prime_UTR 10.0%
!! ATGAGTTTATTAAAATTGAA+GGG - chr8_4:50043532-50043551 None:intergenic 15.0%
!!! CGATGTTTTTTTTTTTTTTA+AGG + chr8_4:50043900-50043919 Msa1330810:intron 15.0%
!!! TTTTTTTTTTTTTGAGATCA+AGG + chr8_4:50044401-50044420 Msa1330810:three_prime_UTR 15.0%
!! ATGAGTTTATTAAAATTGAA+GGG - chr8_4:50043532-50043551 None:intergenic 15.0%
!!! CGATGTTTTTTTTTTTTTTA+AGG + chr8_4:50043900-50043919 Msa1330810:intron 15.0%
!!! TTTTTTTTTTTTTGAGATCA+AGG + chr8_4:50044401-50044420 Msa1330810:three_prime_UTR 15.0%
!! TGAGTTTATTAAAATTGAAG+GGG - chr8_4:50043531-50043550 None:intergenic 20.0%
!! CATGAGTTTATTAAAATTGA+AGG - chr8_4:50043533-50043552 None:intergenic 20.0%
!!! CTTCAATTTTAATAAACTCA+TGG + chr8_4:50043531-50043550 Msa1330810:exon 20.0%
!! ATAAAGATATCTTCATGTAT+AGG + chr8_4:50043809-50043828 Msa1330810:CDS 20.0%
!!! TTCATAATAAGGGTTTTTTA+AGG - chr8_4:50043868-50043887 None:intergenic 20.0%
!! CATGAAAATCTTCATAATAA+GGG - chr8_4:50043878-50043897 None:intergenic 20.0%
!! TCATGAAAATCTTCATAATA+AGG - chr8_4:50043879-50043898 None:intergenic 20.0%
!! AAAATCTAATTCTTCAAACA+AGG + chr8_4:50044077-50044096 Msa1330810:CDS 20.0%
!! TGAGTTTATTAAAATTGAAG+GGG - chr8_4:50043531-50043550 None:intergenic 20.0%
!! CATGAGTTTATTAAAATTGA+AGG - chr8_4:50043533-50043552 None:intergenic 20.0%
!!! CTTCAATTTTAATAAACTCA+TGG + chr8_4:50043531-50043550 Msa1330810:exon 20.0%
!! ATAAAGATATCTTCATGTAT+AGG + chr8_4:50043809-50043828 Msa1330810:CDS 20.0%
!!! TTCATAATAAGGGTTTTTTA+AGG - chr8_4:50043868-50043887 None:intergenic 20.0%
!! CATGAAAATCTTCATAATAA+GGG - chr8_4:50043878-50043897 None:intergenic 20.0%
!! TCATGAAAATCTTCATAATA+AGG - chr8_4:50043879-50043898 None:intergenic 20.0%
!! AAAATCTAATTCTTCAAACA+AGG + chr8_4:50044077-50044096 Msa1330810:CDS 20.0%
!! TATTAAAATTGAAGGGGTTT+TGG - chr8_4:50043525-50043544 None:intergenic 25.0%
! TACAAAATCTATGATAACCA+TGG + chr8_4:50043648-50043667 Msa1330810:CDS 25.0%
! ACAAAATCTATGATAACCAT+GGG + chr8_4:50043649-50043668 Msa1330810:CDS 25.0%
!!! TGATTGATTTTAATTTGTGC+AGG + chr8_4:50043935-50043954 Msa1330810:intron 25.0%
!!! GATTGATTTTAATTTGTGCA+GGG + chr8_4:50043936-50043955 Msa1330810:intron 25.0%
!!! ATTGATTTTAATTTGTGCAG+GGG + chr8_4:50043937-50043956 Msa1330810:intron 25.0%
!! AACTGTAAATGGTTTACTTT+TGG + chr8_4:50044312-50044331 Msa1330810:three_prime_UTR 25.0%
!! AATGAAATGACTCAGTTTTT+GGG + chr8_4:50044369-50044388 Msa1330810:three_prime_UTR 25.0%
!!! TTTTTTTGAGATCAAGGTTT+TGG + chr8_4:50044407-50044426 Msa1330810:three_prime_UTR 25.0%
! ATATAAATTACAAAAGGAGG+AGG - chr8_4:50044437-50044456 None:intergenic 25.0%
!! TCCTCCTTTTGTAATTTATA+TGG + chr8_4:50044436-50044455 Msa1330810:three_prime_UTR 25.0%
!! TATTAAAATTGAAGGGGTTT+TGG - chr8_4:50043525-50043544 None:intergenic 25.0%
! TACAAAATCTATGATAACCA+TGG + chr8_4:50043648-50043667 Msa1330810:CDS 25.0%
! ACAAAATCTATGATAACCAT+GGG + chr8_4:50043649-50043668 Msa1330810:CDS 25.0%
!!! TGATTGATTTTAATTTGTGC+AGG + chr8_4:50043935-50043954 Msa1330810:intron 25.0%
!!! GATTGATTTTAATTTGTGCA+GGG + chr8_4:50043936-50043955 Msa1330810:intron 25.0%
!!! ATTGATTTTAATTTGTGCAG+GGG + chr8_4:50043937-50043956 Msa1330810:intron 25.0%
!! AACTGTAAATGGTTTACTTT+TGG + chr8_4:50044312-50044331 Msa1330810:three_prime_UTR 25.0%
!! AATGAAATGACTCAGTTTTT+GGG + chr8_4:50044369-50044388 Msa1330810:three_prime_UTR 25.0%
!!! TTTTTTTGAGATCAAGGTTT+TGG + chr8_4:50044407-50044426 Msa1330810:three_prime_UTR 25.0%
! ATATAAATTACAAAAGGAGG+AGG - chr8_4:50044437-50044456 None:intergenic 25.0%
!! TCCTCCTTTTGTAATTTATA+TGG + chr8_4:50044436-50044455 Msa1330810:three_prime_UTR 25.0%
AGTATTATTGACATAACCCA+TGG - chr8_4:50043668-50043687 None:intergenic 30.0%
ATCTAGGTGAAGAAACATAA+AGG - chr8_4:50043702-50043721 None:intergenic 30.0%
GAATCTTTCTTCATCTATGA+AGG + chr8_4:50044032-50044051 Msa1330810:CDS 30.0%
AATTCTTCAAACAAGGTTCA+AGG + chr8_4:50044084-50044103 Msa1330810:CDS 30.0%
CAAGATTATTCCTTTCGTAA+AGG + chr8_4:50044108-50044127 Msa1330810:CDS 30.0%
!! AAAAGTGTGTTTCCTTGTTT+TGG + chr8_4:50044335-50044354 Msa1330810:three_prime_UTR 30.0%
AACTGAGTCATTTCATTGAA+GGG - chr8_4:50044366-50044385 None:intergenic 30.0%
AAACTGAGTCATTTCATTGA+AGG - chr8_4:50044367-50044386 None:intergenic 30.0%
! CAATGAAATGACTCAGTTTT+TGG + chr8_4:50044368-50044387 Msa1330810:three_prime_UTR 30.0%
GCCATATAAATTACAAAAGG+AGG - chr8_4:50044440-50044459 None:intergenic 30.0%
CACGCCATATAAATTACAAA+AGG - chr8_4:50044443-50044462 None:intergenic 30.0%
! CTTTTGTAATTTATATGGCG+TGG + chr8_4:50044441-50044460 Msa1330810:three_prime_UTR 30.0%
!! TGGTTTCAGTTTTTCAAAGA+TGG + chr8_4:50044480-50044499 Msa1330810:three_prime_UTR 30.0%
AGATGGAACTGAATATACAA+TGG + chr8_4:50044497-50044516 Msa1330810:three_prime_UTR 30.0%
CCATTTCTATAGATAGATTG+AGG - chr8_4:50044541-50044560 None:intergenic 30.0%
CCTCAATCTATCTATAGAAA+TGG + chr8_4:50044538-50044557 Msa1330810:three_prime_UTR 30.0%
AGTATTATTGACATAACCCA+TGG - chr8_4:50043668-50043687 None:intergenic 30.0%
ATCTAGGTGAAGAAACATAA+AGG - chr8_4:50043702-50043721 None:intergenic 30.0%
GAATCTTTCTTCATCTATGA+AGG + chr8_4:50044032-50044051 Msa1330810:CDS 30.0%
AATTCTTCAAACAAGGTTCA+AGG + chr8_4:50044084-50044103 Msa1330810:CDS 30.0%
CAAGATTATTCCTTTCGTAA+AGG + chr8_4:50044108-50044127 Msa1330810:CDS 30.0%
!! AAAAGTGTGTTTCCTTGTTT+TGG + chr8_4:50044335-50044354 Msa1330810:three_prime_UTR 30.0%
AACTGAGTCATTTCATTGAA+GGG - chr8_4:50044366-50044385 None:intergenic 30.0%
AAACTGAGTCATTTCATTGA+AGG - chr8_4:50044367-50044386 None:intergenic 30.0%
! CAATGAAATGACTCAGTTTT+TGG + chr8_4:50044368-50044387 Msa1330810:three_prime_UTR 30.0%
GCCATATAAATTACAAAAGG+AGG - chr8_4:50044440-50044459 None:intergenic 30.0%
CACGCCATATAAATTACAAA+AGG - chr8_4:50044443-50044462 None:intergenic 30.0%
! CTTTTGTAATTTATATGGCG+TGG + chr8_4:50044441-50044460 Msa1330810:three_prime_UTR 30.0%
!! TGGTTTCAGTTTTTCAAAGA+TGG + chr8_4:50044480-50044499 Msa1330810:three_prime_UTR 30.0%
AGATGGAACTGAATATACAA+TGG + chr8_4:50044497-50044516 Msa1330810:three_prime_UTR 30.0%
CCATTTCTATAGATAGATTG+AGG - chr8_4:50044541-50044560 None:intergenic 30.0%
CCTCAATCTATCTATAGAAA+TGG + chr8_4:50044538-50044557 Msa1330810:three_prime_UTR 30.0%
!!! AATTGAAGGGGTTTTGGTTT+TGG - chr8_4:50043519-50043538 None:intergenic 35.0%
!! TGGTCTTGTTCTAAGTAACA+AGG - chr8_4:50043571-50043590 None:intergenic 35.0%
! TGTTACTTAGAACAAGACCA+CGG + chr8_4:50043571-50043590 Msa1330810:CDS 35.0%
! TTTAGCTTCCTTGAAAGACA+TGG + chr8_4:50043594-50043613 Msa1330810:CDS 35.0%
TATGTTTCTTCACCTAGATC+TGG + chr8_4:50043703-50043722 Msa1330810:CDS 35.0%
TCATAGAATCTTCCAGATCT+AGG - chr8_4:50043718-50043737 None:intergenic 35.0%
TACAAAGAGAACAAGCTTCA+AGG - chr8_4:50043771-50043790 None:intergenic 35.0%
TCTCTTTGTAACAAACCACT+TGG + chr8_4:50043781-50043800 Msa1330810:CDS 35.0%
ATATCTTTATTGCCACCAAG+TGG - chr8_4:50043799-50043818 None:intergenic 35.0%
CTACACTCTTCACTACAAAA+AGG - chr8_4:50043970-50043989 None:intergenic 35.0%
!! TATGAAGGCTTTGAGGAATA+AGG + chr8_4:50044047-50044066 Msa1330810:CDS 35.0%
AATAGCTGTTCCTTTACGAA+AGG - chr8_4:50044121-50044140 None:intergenic 35.0%
!! TAGTTTTGCTTGAAGGGAAA+AGG + chr8_4:50044147-50044166 Msa1330810:exon 35.0%
GAACAAGAGTCAATAGAGAA+GGG + chr8_4:50044272-50044291 Msa1330810:three_prime_UTR 35.0%
GCAGAGAATGTAACTGTAAA+TGG + chr8_4:50044301-50044320 Msa1330810:three_prime_UTR 35.0%
!!! AATTGAAGGGGTTTTGGTTT+TGG - chr8_4:50043519-50043538 None:intergenic 35.0%
!! TGGTCTTGTTCTAAGTAACA+AGG - chr8_4:50043571-50043590 None:intergenic 35.0%
! TGTTACTTAGAACAAGACCA+CGG + chr8_4:50043571-50043590 Msa1330810:CDS 35.0%
! TTTAGCTTCCTTGAAAGACA+TGG + chr8_4:50043594-50043613 Msa1330810:CDS 35.0%
TATGTTTCTTCACCTAGATC+TGG + chr8_4:50043703-50043722 Msa1330810:CDS 35.0%
TCATAGAATCTTCCAGATCT+AGG - chr8_4:50043718-50043737 None:intergenic 35.0%
TACAAAGAGAACAAGCTTCA+AGG - chr8_4:50043771-50043790 None:intergenic 35.0%
TCTCTTTGTAACAAACCACT+TGG + chr8_4:50043781-50043800 Msa1330810:CDS 35.0%
ATATCTTTATTGCCACCAAG+TGG - chr8_4:50043799-50043818 None:intergenic 35.0%
CTACACTCTTCACTACAAAA+AGG - chr8_4:50043970-50043989 None:intergenic 35.0%
!! TATGAAGGCTTTGAGGAATA+AGG + chr8_4:50044047-50044066 Msa1330810:CDS 35.0%
AATAGCTGTTCCTTTACGAA+AGG - chr8_4:50044121-50044140 None:intergenic 35.0%
!! TAGTTTTGCTTGAAGGGAAA+AGG + chr8_4:50044147-50044166 Msa1330810:exon 35.0%
GAACAAGAGTCAATAGAGAA+GGG + chr8_4:50044272-50044291 Msa1330810:three_prime_UTR 35.0%
GCAGAGAATGTAACTGTAAA+TGG + chr8_4:50044301-50044320 Msa1330810:three_prime_UTR 35.0%
TGCAACATCCATGTCTTTCA+AGG - chr8_4:50043605-50043624 None:intergenic 40.0%
ATGGATGTTGCAAGTTATCG+TGG + chr8_4:50043613-50043632 Msa1330810:CDS 40.0%
AGAACAAGCTTCAAGGAAGT+GGG - chr8_4:50043764-50043783 None:intergenic 40.0%
CTTTGTAACAAACCACTTGG+TGG + chr8_4:50043784-50043803 Msa1330810:CDS 40.0%
CTTCATCTATGAAGGCTTTG+AGG + chr8_4:50044040-50044059 Msa1330810:CDS 40.0%
!! TGCAGCATAGTTTTGCTTGA+AGG + chr8_4:50044140-50044159 Msa1330810:exon 40.0%
!! GCAGCATAGTTTTGCTTGAA+GGG + chr8_4:50044141-50044160 Msa1330810:exon 40.0%
CGAACAAGAGTCAATAGAGA+AGG + chr8_4:50044271-50044290 Msa1330810:three_prime_UTR 40.0%
TGAAGGGATCTTCCAAAACA+AGG - chr8_4:50044350-50044369 None:intergenic 40.0%
GTGGTTGTGTTGTGCTTATA+TGG + chr8_4:50044460-50044479 Msa1330810:three_prime_UTR 40.0%
TGCAACATCCATGTCTTTCA+AGG - chr8_4:50043605-50043624 None:intergenic 40.0%
ATGGATGTTGCAAGTTATCG+TGG + chr8_4:50043613-50043632 Msa1330810:CDS 40.0%
AGAACAAGCTTCAAGGAAGT+GGG - chr8_4:50043764-50043783 None:intergenic 40.0%
CTTTGTAACAAACCACTTGG+TGG + chr8_4:50043784-50043803 Msa1330810:CDS 40.0%
CTTCATCTATGAAGGCTTTG+AGG + chr8_4:50044040-50044059 Msa1330810:CDS 40.0%
!! TGCAGCATAGTTTTGCTTGA+AGG + chr8_4:50044140-50044159 Msa1330810:exon 40.0%
!! GCAGCATAGTTTTGCTTGAA+GGG + chr8_4:50044141-50044160 Msa1330810:exon 40.0%
CGAACAAGAGTCAATAGAGA+AGG + chr8_4:50044271-50044290 Msa1330810:three_prime_UTR 40.0%
TGAAGGGATCTTCCAAAACA+AGG - chr8_4:50044350-50044369 None:intergenic 40.0%
GTGGTTGTGTTGTGCTTATA+TGG + chr8_4:50044460-50044479 Msa1330810:three_prime_UTR 40.0%
!!! AGGGGTTTTGGTTTTGGTTG+AGG - chr8_4:50043513-50043532 None:intergenic 45.0%
CTTTCAAGGAAGCTAAACCG+TGG - chr8_4:50043591-50043610 None:intergenic 45.0%
GAACAAGCTTCAAGGAAGTG+GGG - chr8_4:50043763-50043782 None:intergenic 45.0%
GAGAACAAGCTTCAAGGAAG+TGG - chr8_4:50043765-50043784 None:intergenic 45.0%
!!! GGGGTTTTGGTTTTGGTTGA+GGG - chr8_4:50043512-50043531 None:intergenic 45.0%
!!! AGGGGTTTTGGTTTTGGTTG+AGG - chr8_4:50043513-50043532 None:intergenic 45.0%
CTTTCAAGGAAGCTAAACCG+TGG - chr8_4:50043591-50043610 None:intergenic 45.0%
GAACAAGCTTCAAGGAAGTG+GGG - chr8_4:50043763-50043782 None:intergenic 45.0%
GAGAACAAGCTTCAAGGAAG+TGG - chr8_4:50043765-50043784 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 50043510 50044577 50043510 ID=Msa1330810;Name=Msa1330810
chr8_4 mRNA 50043510 50044577 50043510 ID=Msa1330810-mRNA-1;Parent=Msa1330810;Name=Msa1330810-mRNA-1;_AED=0.06;_eAED=0.06;_QI=40|1|1|1|1|1|2|428|157
chr8_4 exon 50043510 50043830 50043510 ID=Msa1330810-mRNA-1:exon:14542;Parent=Msa1330810-mRNA-1
chr8_4 exon 50043957 50044577 50043957 ID=Msa1330810-mRNA-1:exon:14543;Parent=Msa1330810-mRNA-1
chr8_4 five_prime_UTR 50043510 50043549 50043510 ID=Msa1330810-mRNA-1:five_prime_utr;Parent=Msa1330810-mRNA-1
chr8_4 CDS 50043550 50043830 50043550 ID=Msa1330810-mRNA-1:cds;Parent=Msa1330810-mRNA-1
chr8_4 CDS 50043957 50044149 50043957 ID=Msa1330810-mRNA-1:cds;Parent=Msa1330810-mRNA-1
chr8_4 three_prime_UTR 50044150 50044577 50044150 ID=Msa1330810-mRNA-1:three_prime_utr;Parent=Msa1330810-mRNA-1
Gene Sequence

>Msa1330810

ATGGACTCATCAATAAAACCTTGTTACTTAGAACAAGACCACGGTTTAGCTTCCTTGAAAGACATGGATGTTGCAAGTTATCGTGGCAATAGTTTTGTTACAAAATCTATGATAACCATGGGTTATGTCAATAATACTAGTTTCAAGAACCTTTATGTTTCTTCACCTAGATCTGGAAGATTCTATGATGCTAGATTTGAAGATCATCAACCCCACTTCCTTGAAGCTTGTTCTCTTTGTAACAAACCACTTGGTGGCAATAAAGATATCTTCATGTATAGGGGTGACACACCTTTTTGTAGTGAAGAGTGTAGACAAGAGCAAATAGAGATAGATGAATTGAAAGAAAAAAATATGAATCTTTCTTCATCTATGAAGGCTTTGAGGAATAAGGAGCATAGAAAATCTAATTCTTCAAACAAGGTTCAAGGACAAGATTATTCCTTTCGTAAAGGAACAGCTATTGCAGCATAG
Protein sequence

>Msa1330810

MDSSIKPCYLEQDHGLASLKDMDVASYRGNSFVTKSMITMGYVNNTSFKNLYVSSPRSGRFYDARFEDHQPHFLEACSLCNKPLGGNKDIFMYRGDTPFCSEECRQEQIEIDELKEKNMNLSSSMKALRNKEHRKSNSSNKVQGQDYSFRKGTAIAA*