AlfalfaGEDB | Msa1261260

M. sativa cultivar zhongmu-4 / Msa1261260

KEGG: -
GO: GO:0001101 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008213 GO:0009266 GO:0009628 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010452 GO:0010468 GO:0010476 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032870 GO:0033993 GO:0034968 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045934 GO:0048519 GO:0048523 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0140110 GO:1901564 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000026 GO:2000028 GO:2000112 GO:2000241 GO:2000242 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261260	XP_003609208.1	98.157	434	8	0	1	434	1	434	0.0	887

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261260	sp\|Q9ZQ85\|EFM_ARATH	50.959	469	158	20	1	434	1	432	4.87e-117	352

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261260	G7JU63	98.157	434	8	0	1	434	1	434	0.0	887

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TFs/TRs:

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Gene ID	Type	Classification
Msa1261260	TF	GARP-G2-like

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1214280	Msa1261260	0.820731	5.873853e-53	-8.615850e-47
Msa1261260	Msa1261460	0.804943	1.694478e-49	-8.615850e-47
Msa1261260	Msa1304170	0.809276	2.050065e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261260	MtrunA17_Chr4g0062921	98.157	434	8	0	1	434	1	434	0.0	887
Msa1261260	MtrunA17_Chr4g0045361	38.649	370	171	12	4	328	6	364	1.16e-61	205
Msa1261260	MtrunA17_Chr5g0422621	40.176	341	141	16	30	350	12	309	2.58e-51	177
Msa1261260	MtrunA17_Chr1g0198601	35.417	336	152	8	29	328	2	308	4.57e-50	174
Msa1261260	MtrunA17_Chr5g0402631	34.539	304	146	8	6	305	31	285	2.57e-37	139
Msa1261260	MtrunA17_Chr1g0175081	43.023	86	45	1	219	300	2	87	2.87e-15	71.2
Msa1261260	MtrunA17_Chr1g0190301	48.235	85	42	1	226	310	166	248	3.36e-15	77.0
Msa1261260	MtrunA17_Chr7g0261911	47.727	88	44	1	226	313	253	338	6.37e-15	76.6
Msa1261260	MtrunA17_Chr2g0304841	37.383	107	59	3	232	331	210	315	8.37e-15	76.6
Msa1261260	MtrunA17_Chr4g0074411	49.296	71	35	1	232	302	198	267	3.79e-14	74.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261260	AT2G03500.1	50.959	469	158	20	1	434	1	432	4.95e-118	352
Msa1261260	AT1G68670.1	37.815	357	156	12	32	351	13	340	1.47e-61	204
Msa1261260	AT1G25550.1	39.697	330	131	13	18	316	2	294	1.78e-55	187
Msa1261260	AT3G25790.1	33.613	357	140	8	19	333	1	302	3.11e-45	160
Msa1261260	AT1G25550.2	38.305	295	118	11	18	285	2	259	3.35e-43	153
Msa1261260	AT4G37180.1	35.452	299	135	9	7	293	16	268	6.65e-39	144
Msa1261260	AT4G37180.2	34.797	296	148	8	7	293	16	275	3.70e-37	139
Msa1261260	AT4G37180.3	34.708	291	132	9	7	285	16	260	3.43e-34	129
Msa1261260	AT1G13300.1	71.765	85	22	1	234	316	180	264	7.12e-34	130
Msa1261260	AT3G25790.2	32.281	285	136	6	19	285	1	246	9.75e-30	116

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Find 134 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCACCAACTGTGCTGC+TGG	0.488784	8_2:+63574707	None:intergenic
AAACTCATAGGAAAGCAAGA+AGG	0.642113	8_2:-63575646	Msa1261260:CDS
AAATAATAATGTATGTGATA+AGG	0.450078	8_2:-63576026	Msa1261260:CDS
AAATAATGTAATCATTATTA+AGG	0.242268	8_2:+63574303	None:intergenic
AACCCGTCTCCACATGTCTC+CGG	0.479058	8_2:+63574458	None:intergenic
AACTCCTCTTGTCAAGGACA+AGG	0.622383	8_2:-63575833	Msa1261260:CDS
AACTGTAGAAACTAATTTAT+CGG	0.335462	8_2:-63573823	None:intergenic
AAGAATCAGATTCTGACTCT+TGG	0.420642	8_2:-63573977	Msa1261260:three_prime_UTR
AAGACCACGCCACACGGCCA+GGG	0.575388	8_2:-63574485	Msa1261260:CDS
AAGACCTTGTCCTTGACAAG+AGG	0.632597	8_2:+63575829	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAGAGATATATAAAAGGGA+AGG	+	chr8_2:63573837-63573856	None:intergenic	30.0%
ACGGAGAGAGATATATAAAA+GGG	+	chr8_2:63573841-63573860	None:intergenic	30.0%
TGACAAAATAGAAAAGAAGG+TGG	+	chr8_2:63573946-63573965	None:intergenic	30.0%
TCCATGTTACTGAAATCATT+TGG	-	chr8_2:63574072-63574091	Msa1261260:three_prime_UTR	30.0%
TCCAAATGATTTCAGTAACA+TGG	+	chr8_2:63574076-63574095	None:intergenic	30.0%
ATGTATGTGATAAGGCAAAT+TGG	-	chr8_2:63574500-63574519	Msa1261260:CDS	30.0%
TTGTTCCTAATTCACTTGTT+TGG	+	chr8_2:63574549-63574568	None:intergenic	30.0%
ACAATTCAAACCAAACTCAT+AGG	-	chr8_2:63574860-63574879	Msa1261260:intron	30.0%
TAATTCTCTCTCTCAGTTTA+AGG	-	chr8_2:63575567-63575586	Msa1261260:intron	30.0%
TTAAAAAGGACAGAAAAGTG+AGG	-	chr8_2:63576446-63576465	Msa1261260:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	63573824	63576716	63573824	ID=Msa1261260;Name=Msa1261260
chr8_2	mRNA	63573824	63576716	63573824	ID=Msa1261260-mRNA-1;Parent=Msa1261260;Name=Msa1261260-mRNA-1;_AED=0.03;_eAED=0.03;_QI=200\|1\|1\|1\|1\|1\|4\|507\|434
chr8_2	exon	63573824	63574780	63573824	ID=Msa1261260-mRNA-1:exon:20335;Parent=Msa1261260-mRNA-1
chr8_2	exon	63574975	63575051	63574975	ID=Msa1261260-mRNA-1:exon:20334;Parent=Msa1261260-mRNA-1
chr8_2	exon	63575582	63576169	63575582	ID=Msa1261260-mRNA-1:exon:20333;Parent=Msa1261260-mRNA-1
chr8_2	exon	63576327	63576716	63576327	ID=Msa1261260-mRNA-1:exon:20332;Parent=Msa1261260-mRNA-1
chr8_2	five_prime_UTR	63576517	63576716	63576517	ID=Msa1261260-mRNA-1:five_prime_utr;Parent=Msa1261260-mRNA-1
chr8_2	CDS	63576327	63576516	63576327	ID=Msa1261260-mRNA-1:cds;Parent=Msa1261260-mRNA-1
chr8_2	CDS	63575582	63576169	63575582	ID=Msa1261260-mRNA-1:cds;Parent=Msa1261260-mRNA-1
chr8_2	CDS	63574975	63575051	63574975	ID=Msa1261260-mRNA-1:cds;Parent=Msa1261260-mRNA-1
chr8_2	CDS	63574331	63574780	63574331	ID=Msa1261260-mRNA-1:cds;Parent=Msa1261260-mRNA-1
chr8_2	three_prime_UTR	63573824	63574330	63573824	ID=Msa1261260-mRNA-1:three_prime_utr;Parent=Msa1261260-mRNA-1

Gene Sequence

>Msa1261260

ATGGCATCACCATCTGAACTAGGCTTGGATTGCAAGCCACATAGCTACTCCATGTTACTGAAATCATTTGGAGAACAAAGTGATCAAAGCTACAAACTCGAAGAATTTGTTTCTCGCTTGGAAGAAGAACGTCTCAAAATCGATGCTTTCAAACGTGAGCTTCCTCTTTGCATGCAACTTCTCACAAACGCGATGGAAGCTTCGAAGCAGCAACTTCAAGCGTTCAGATCGAATCAAGGAGCAAAACCAATCCTAGAAGAATTCATACCAGTGAAGCAATTATCATCATCAGAAACATTGGAAAAGACAACAAATAATAATGTATGTGATAAGGCAAATTGGATGACAAGTGCACAATTATGGAGCCAAACAAGTGAATTAGGAACAAAACAACAACAAAATTCCACAAAAGAAATTAATGATAACAATAACAATATTGGATTTAACATTAGTCCTAAACATAGAAATGGTGGAGCCTTTTTACCATTCTCTAAAGAAAGAAATAACTCCTCTTGTCAAGGACAAGGTCTTCCTGAACTAGCACTAGCTTCTACTCAAAAAGAAGAGGATAAGAAACATGCTGGAGAGGTAGAAAAAGGTAAAACAAATTCAGGGAATGAAGTTGATCATCAAGGAAAAGGGAGTCCAGTAGCATCATCACAAACTCAGACAACAAGCAACAATTCAAACCAAACTCATAGGAAAGCAAGAAGGTGTTGGTCACCAGACTTGCACCGTAGATTTGTTAATGCTCTTCAAATGCTTGGTGGATCTCAAGTGGCAACACCAAAACAGATAAGGGAGTTGATGAAGGTTGATGGTTTGACCAATGATGAAGTTAAAAGCCATCTGCAGAAATATAGGCTACACACAAGAAGACCAAGTCCAAGTGCACAAAATGGTGCACCAGCAGCACAGTTGGTGGTTTTAGGAGGAATATGGGTCCCACCAGAATATGCAACTGCAGCACATGCAGGAGGAACCCCTACTCTCTATGGTGGTCATCCTACTTCTCATCATCTCACTCTGCCACATTACTGCACTGCTCCAGGCCAAGATCAGTACTACACTACTGCACCGCCGCCGCAGCAGCTACTTCCGCCACCTCATCACATGCACCATGTCTACAAGACCACGCCACACGGCCAGGGCTCACCGGAGACATGTGGAGACGGGTTGGAGAGTATCGAAAATGGGAAGACGTCGGAGAGTAGTAGCTGGAAAGAGGGGAGTTCTGAGGATGAAGGGGAGAGGAAAGGGTTTGTTGAAGAGAGTAATGGGAGTGAAATCACTCTCAAATTTTAA

Protein sequence

>Msa1261260

MASPSELGLDCKPHSYSMLLKSFGEQSDQSYKLEEFVSRLEEERLKIDAFKRELPLCMQLLTNAMEASKQQLQAFRSNQGAKPILEEFIPVKQLSSSETLEKTTNNNVCDKANWMTSAQLWSQTSELGTKQQQNSTKEINDNNNNIGFNISPKHRNGGAFLPFSKERNNSSCQGQGLPELALASTQKEEDKKHAGEVEKGKTNSGNEVDHQGKGSPVASSQTQTTSNNSNQTHRKARRCWSPDLHRRFVNALQMLGGSQVATPKQIRELMKVDGLTNDEVKSHLQKYRLHTRRPSPSAQNGAPAAQLVVLGGIWVPPEYATAAHAGGTPTLYGGHPTSHHLTLPHYCTAPGQDQYYTTAPPPQQLLPPPHHMHHVYKTTPHGQGSPETCGDGLESIENGKTSESSSWKEGSSEDEGERKGFVEESNGSEITLKF*