AlfalfaGEDB | Msa1261460

M. sativa cultivar zhongmu-4 / Msa1261460

KEGG: -
GO: GO:0001101 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008213 GO:0009266 GO:0009628 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010452 GO:0010468 GO:0010476 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032870 GO:0033993 GO:0034968 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045934 GO:0048519 GO:0048523 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0140110 GO:1901564 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000026 GO:2000028 GO:2000112 GO:2000241 GO:2000242 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261460	XP_003609208.1	98.157	434	8	0	1	434	1	434	0.0	887

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261460	sp\|Q9ZQ85\|EFM_ARATH	50.959	469	158	20	1	434	1	432	4.56e-117	352

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261460	G7JU63	98.157	434	8	0	1	434	1	434	0.0	887

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TFs/TRs:

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Gene ID	Type	Classification
Msa1261460	TF	GARP-G2-like

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1214280	Msa1261460	0.973677	7.774552e-137	-8.615850e-47
Msa1261260	Msa1261460	0.804943	1.694478e-49	-8.615850e-47
Msa1261460	Msa1304170	0.957598	1.844612e-115	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261460	MtrunA17_Chr4g0062921	98.157	434	8	0	1	434	1	434	0.0	887
Msa1261460	MtrunA17_Chr4g0045361	38.649	370	171	12	4	328	6	364	1.87e-61	205
Msa1261460	MtrunA17_Chr5g0422621	40.469	341	140	16	30	350	12	309	1.00e-51	177
Msa1261460	MtrunA17_Chr1g0198601	35.714	336	151	8	29	328	2	308	1.43e-50	175
Msa1261460	MtrunA17_Chr5g0402631	34.539	304	146	8	6	305	31	285	1.97e-37	139
Msa1261460	MtrunA17_Chr1g0175081	43.023	86	45	1	219	300	2	87	2.87e-15	71.2
Msa1261460	MtrunA17_Chr1g0190301	48.235	85	42	1	226	310	166	248	3.24e-15	77.0
Msa1261460	MtrunA17_Chr7g0261911	47.727	88	44	1	226	313	253	338	6.67e-15	76.6
Msa1261460	MtrunA17_Chr2g0304841	37.383	107	59	3	232	331	210	315	8.52e-15	76.6
Msa1261460	MtrunA17_Chr4g0074411	49.296	71	35	1	232	302	198	267	3.79e-14	74.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1261460	AT2G03500.1	50.959	469	158	20	1	434	1	432	4.64e-118	352
Msa1261460	AT1G68670.1	37.535	357	157	12	32	351	13	340	3.87e-61	202
Msa1261460	AT1G25550.1	39.394	330	132	13	18	316	2	294	4.77e-55	186
Msa1261460	AT3G25790.1	33.333	357	141	8	19	333	1	302	7.85e-45	160
Msa1261460	AT1G25550.2	37.966	295	119	11	18	285	2	259	8.33e-43	152
Msa1261460	AT4G37180.1	35.473	296	139	8	7	293	16	268	2.51e-39	145
Msa1261460	AT4G37180.2	34.797	296	148	8	7	293	16	275	5.18e-37	139
Msa1261460	AT4G37180.3	34.722	288	136	8	7	285	16	260	1.03e-34	130
Msa1261460	AT1G13300.1	71.765	85	22	1	234	316	180	264	6.84e-34	130
Msa1261460	AT1G49560.1	64.706	85	25	2	231	314	188	268	1.23e-27	112

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Find 127 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTACTAGCTGTGCTGC+TGG	0.531932	8_2:-63836827	None:intergenic
AAACTCATAGGAAAGCAAGA+AGG	0.642113	8_2:+63835883	Msa1261460:CDS
AAATAATAATGTATGTGATA+AGG	0.450078	8_2:+63835503	Msa1261460:CDS
AAATAATGTAATCATTATTA+AGG	0.242268	8_2:-63837231	None:intergenic
AACCCGTCTCCACATGTCTC+CGG	0.479058	8_2:-63837076	None:intergenic
AACTCCTCTTGTCAAGGACA+AGG	0.622383	8_2:+63835696	Msa1261460:CDS
AACTGTAGAAACTAATTTAT+CGG	0.335462	8_2:+63837700	Msa1261460:three_prime_UTR
AAGAATCAGATTCTGACTCT+TGG	0.420642	8_2:+63837546	Msa1261460:three_prime_UTR
AAGACCACGCCACACGGCCA+AGG	0.603883	8_2:+63837049	Msa1261460:CDS
AAGACCTTGTCCTTGACAAG+AGG	0.632597	8_2:-63835700	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAGAGATATATAAAAGGGA+AGG	-	chr8_2:63834848-63834867	None:intergenic	30.0%
GACAGAGAGAGATATATAAA+AGG	-	chr8_2:63834853-63834872	None:intergenic	30.0%
TGACAAAATAGAAAAGAAGG+TGG	-	chr8_2:63834957-63834976	None:intergenic	30.0%
TCCATGTTACTGAAATCATT+TGG	+	chr8_2:63835083-63835102	Msa1261460:CDS	30.0%
TCCAAATGATTTCAGTAACA+TGG	-	chr8_2:63835087-63835106	None:intergenic	30.0%
TGTCAGATAAGATTTGAACT+CGG	-	chr8_2:63835300-63835319	None:intergenic	30.0%
ATTAGCATCATCAGAAACAT+TGG	+	chr8_2:63835470-63835489	Msa1261460:CDS	30.0%
ATGTATGTGATAAGGCAAAT+TGG	+	chr8_2:63835511-63835530	Msa1261460:CDS	30.0%
TTGTTCCTAATTCACTTGTT+TGG	-	chr8_2:63835560-63835579	None:intergenic	30.0%
ACAATTCAAACCAAACTCAT+AGG	+	chr8_2:63835871-63835890	Msa1261460:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	63834835	63837721	63834835	ID=Msa1261460;Name=Msa1261460
chr8_2	mRNA	63834835	63837721	63834835	ID=Msa1261460-mRNA-1;Parent=Msa1261460;Name=Msa1261460-mRNA-1;_AED=0.02;_eAED=0.02;_QI=200\|1\|1\|1\|1\|1\|4\|496\|434
chr8_2	exon	63834835	63835224	63834835	ID=Msa1261460-mRNA-1:exon:20588;Parent=Msa1261460-mRNA-1
chr8_2	exon	63835382	63835969	63835382	ID=Msa1261460-mRNA-1:exon:20589;Parent=Msa1261460-mRNA-1
chr8_2	exon	63836500	63836576	63836500	ID=Msa1261460-mRNA-1:exon:20590;Parent=Msa1261460-mRNA-1
chr8_2	exon	63836776	63837721	63836776	ID=Msa1261460-mRNA-1:exon:20591;Parent=Msa1261460-mRNA-1
chr8_2	five_prime_UTR	63834835	63835034	63834835	ID=Msa1261460-mRNA-1:five_prime_utr;Parent=Msa1261460-mRNA-1
chr8_2	CDS	63835035	63835224	63835035	ID=Msa1261460-mRNA-1:cds;Parent=Msa1261460-mRNA-1
chr8_2	CDS	63835382	63835969	63835382	ID=Msa1261460-mRNA-1:cds;Parent=Msa1261460-mRNA-1
chr8_2	CDS	63836500	63836576	63836500	ID=Msa1261460-mRNA-1:cds;Parent=Msa1261460-mRNA-1
chr8_2	CDS	63836776	63837225	63836776	ID=Msa1261460-mRNA-1:cds;Parent=Msa1261460-mRNA-1
chr8_2	three_prime_UTR	63837226	63837721	63837226	ID=Msa1261460-mRNA-1:three_prime_utr;Parent=Msa1261460-mRNA-1

Gene Sequence

>Msa1261460

ATGGCTTCACCATCTGAACTAGGCTTGGATTGCAAGCCACATAGCTACTCCATGTTACTGAAATCATTTGGAGAACAAAGTGATCAAAGCTACAAACTTGAAGAGTTTGTTTCTCGCTTAGAAGAAGAACGTCTCAAAATTGATGCTTTCAAACGTGAGCTTCCTCTCTGCATGCAACTTCTCACAAACGCGATGGAAGCATCGAAGCAGCAACTTCAAGCGTTTAGATCGAATCAAGGAGCAAAACCAATTCTAGAAGAATTCATACCAGTGAAGCAATTAGCATCATCAGAAACATTGGAAAAGACAACAAATAATAATGTATGTGATAAGGCAAATTGGATGACAAGTGCACAATTATGGAGCCAAACAAGTGAATTAGGAACAAAACAACAACAAAATTCCACAAAAGAAATTAATGATAACAATAACAATATTGGATTTAACATTAGTCCTAAACATAGAAATGGTGGAGCCTTTTTACCATTCTCAAAAGAAAGAAATAACTCCTCTTGTCAAGGACAAGGTCTTCCTGAACTAGCACTAGCTTCTACTCAAAAAGAAGAGGATAAGAAACATGCTGGAGAGGTAGAAAAAGGTAAAACAAATTCAGGGAATGAAGTTGATCATCAAGGAAAAGGGAGTCCAGTAGCATCATCACAAACTCAGACAACAAGCAACAATTCAAACCAAACTCATAGGAAAGCAAGAAGGTGTTGGTCACCAGACTTGCACCGTAGATTTGTTAATGCTCTTCAAATGCTTGGTGGATCTCAAGTGGCAACACCAAAACAGATAAGGGAGTTGATGAAGGTTGATGGTTTGACCAATGATGAAGTTAAAAGCCATCTGCAGAAATATAGGCTACACACAAGAAGACCAAGTCCAAGTGCACAAAATGGTGCACCAGCAGCACAGCTAGTAGTTTTAGGAGGAATATGGGTCCCACCAGAATATGCAACTGCAGCACATGCAGGAGGAACCCCTACTCTCTATGGTGGTCATCCTACTTCTCATCATCTCACTCTGCCACATTACTGCACTGCTCCAGGCCAAGATCAGTACTACACTACTGCACCGCCGCCGCAGCAGCTACTTCCGCCACCTCATCACATGCACCATGTCTACAAGACCACGCCACACGGCCAAGGTTCACCGGAGACATGTGGAGACGGGTTGGAGAGTATCGAAAATGGAAAGACGTCGGAGAGTAGTAGCTGGAAAGAGGGGAGTTCTGAGGATGAAGGGGAGAGGAAAGGGTTTGTTGAAGAGAGCAATGGGAGTGAAATCACTCTCAAATTTTAA

Protein sequence

>Msa1261460

MASPSELGLDCKPHSYSMLLKSFGEQSDQSYKLEEFVSRLEEERLKIDAFKRELPLCMQLLTNAMEASKQQLQAFRSNQGAKPILEEFIPVKQLASSETLEKTTNNNVCDKANWMTSAQLWSQTSELGTKQQQNSTKEINDNNNNIGFNISPKHRNGGAFLPFSKERNNSSCQGQGLPELALASTQKEEDKKHAGEVEKGKTNSGNEVDHQGKGSPVASSQTQTTSNNSNQTHRKARRCWSPDLHRRFVNALQMLGGSQVATPKQIRELMKVDGLTNDEVKSHLQKYRLHTRRPSPSAQNGAPAAQLVVLGGIWVPPEYATAAHAGGTPTLYGGHPTSHHLTLPHYCTAPGQDQYYTTAPPPQQLLPPPHHMHHVYKTTPHGQGSPETCGDGLESIENGKTSESSSWKEGSSEDEGERKGFVEESNGSEITLKF*