AlfalfaGEDB | Msa0813610

M. sativa cultivar zhongmu-4 / Msa0813610

KEGG: K00512|K13029
GO: GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009408 GO:0009628 GO:0010286 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0050896 GO:0055114
PFAM: PF00067.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0813610	XP_003615846.2	96.647	507	16	1	1	507	1	506	0.0	1018

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0813610	sp\|O65782\|C83B1_ARATH	46.154	494	257	5	14	504	12	499	4.70e-156	456

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0813610	G7JZM8	96.647	507	16	1	1	507	1	506	0.0	1018

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0004180	Msa0813610	-0.850829	1.318024e-60	-8.615850e-47
Msa0005560	Msa0813610	0.811933	5.462711e-51	-8.615850e-47
Msa0011010	Msa0813610	0.812017	5.236989e-51	-8.615850e-47
Msa0011040	Msa0813610	0.809739	1.630609e-50	-8.615850e-47
Msa0011510	Msa0813610	0.820465	6.761361e-53	-8.615850e-47
Msa0015070	Msa0813610	0.824558	7.565068e-54	-8.615850e-47
Msa0016380	Msa0813610	-0.803138	4.022262e-49	-8.615850e-47
Msa0017710	Msa0813610	0.812974	3.236582e-51	-8.615850e-47
Msa0018110	Msa0813610	-0.810902	9.150854e-51	-8.615850e-47
Msa0021240	Msa0813610	-0.821225	4.522194e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0813610	MtrunA17_Chr5g0431511	96.647	507	16	1	1	507	1	506	1018
Msa0813610	MtrunA17_Chr5g0431501	83.966	474	76	0	34	507	33	506	839
Msa0813610	MtrunA17_Chr5g0431461	83.966	474	76	0	34	507	33	506	839
Msa0813610	MtrunA17_Chr7g0218601	54.601	489	215	4	19	504	16	500	563
Msa0813610	MtrunA17_Chr5g0419691	54.709	499	214	4	10	501	1	494	556
Msa0813610	MtrunA17_Chr4g0011871	54.000	500	217	5	10	501	1	495	549
Msa0813610	MtrunA17_Chr7g0218611	54.865	483	214	3	24	504	21	501	548
Msa0813610	MtrunA17_Chr4g0011961	52.811	498	228	4	5	501	2	493	548
Msa0813610	MtrunA17_Chr4g0011921	53.119	497	227	4	6	501	3	494	545
Msa0813610	MtrunA17_Chr4g0011881	55.215	489	212	4	13	501	13	494	538

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0813610	AT4G31500.1	46.154	494	257	5	14	504	12	499	4.77e-157	456
Msa0813610	AT3G26300.1	44.960	496	263	5	9	502	9	496	1.92e-149	437
Msa0813610	AT3G26310.1	45.000	500	264	5	9	506	9	499	4.73e-148	433
Msa0813610	AT5G57260.1	45.175	487	255	6	24	504	21	501	1.68e-143	422
Msa0813610	AT3G26330.1	45.106	470	251	3	35	502	32	496	2.20e-143	421
Msa0813610	AT5G25180.1	43.443	488	261	6	18	499	11	489	6.81e-143	420
Msa0813610	AT5G25120.1	42.418	488	272	4	14	499	9	489	1.54e-142	419
Msa0813610	AT5G25140.1	41.463	492	276	5	13	499	5	489	7.17e-141	415
Msa0813610	AT5G25130.1	44.161	471	258	3	29	499	24	489	3.46e-140	413
Msa0813610	AT4G13770.1	41.498	494	279	7	13	502	11	498	1.49e-138	409

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Find 125 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATGGCAATTGCTACTG+TGG	0.581106	5_3:+44610499	Msa0813610:CDS
AAAGGAAGGCCTTTAGGACC+TGG	0.558362	5_3:-44608314	None:intergenic
AAATCAACAACTCTTCCACC+AGG	0.681204	5_3:+44608296	Msa0813610:CDS
AAATGCCTGAAGGAGTAAAG+TGG	0.595294	5_3:+44610561	Msa0813610:CDS
AACATATAATACAAGGGAAA+TGG	0.418978	5_3:-44610756	None:intergenic
AAGAATAGCAAGAGAGCAAA+AGG	0.412650	5_3:-44608242	None:intergenic
AATATTGCAGAATATGTTGC+TGG	0.455781	5_3:+44610049	Msa0813610:intron
AATCGCGGCCGCAGTAGTTT+GGG	0.411457	5_3:+44610085	Msa0813610:CDS
AATGCCTGAAGGAGTAAAGT+GGG	0.643490	5_3:+44610562	Msa0813610:CDS
ACACATCAAACCCTTGATCA+TGG	0.552900	5_3:+44609093	Msa0813610:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATGGAAAATATTAAGGGAG+TGG	-	chr5_3:44608126-44608145	None:intergenic	30.0%
AGCAAAAGGAAAAAGAAGAA+GGG	-	chr5_3:44608231-44608250	None:intergenic	30.0%
CAAGTTTAAGGGAGATTATA+GGG	-	chr5_3:44608412-44608431	None:intergenic	30.0%
TTTCATATGGCTGATTAAAG+CGG	-	chr5_3:44608519-44608538	None:intergenic	30.0%
CTCTATATGGTGAAAAAAAC+TGG	-	chr5_3:44608562-44608581	None:intergenic	30.0%
TGTCTCCAATATTCTCTATA+TGG	-	chr5_3:44608575-44608594	None:intergenic	30.0%
ATGAACATCTTGATCCAAAT+AGG	+	chr5_3:44608981-44609000	Msa0813610:CDS	30.0%
TCAATTACATCATCTTCATG+TGG	-	chr5_3:44609019-44609038	None:intergenic	30.0%
CTCTTATTAAGCCTTAATCT+TGG	+	chr5_3:44609147-44609166	Msa0813610:intron	30.0%
AATATTGCAGAATATGTTGC+TGG	+	chr5_3:44610049-44610068	Msa0813610:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	44608087	44610911	44608087	ID=Msa0813610;Name=Msa0813610
chr5_3	mRNA	44608087	44610911	44608087	ID=Msa0813610-mRNA-1;Parent=Msa0813610;Name=Msa0813610-mRNA-1;_AED=0.00;_eAED=0.00;_QI=122\|1\|1\|1\|1\|1\|2\|235\|507
chr5_3	exon	44608087	44609114	44608087	ID=Msa0813610-mRNA-1:exon:13729;Parent=Msa0813610-mRNA-1
chr5_3	exon	44610059	44610911	44610059	ID=Msa0813610-mRNA-1:exon:13730;Parent=Msa0813610-mRNA-1
chr5_3	five_prime_UTR	44608087	44608208	44608087	ID=Msa0813610-mRNA-1:five_prime_utr;Parent=Msa0813610-mRNA-1
chr5_3	CDS	44608209	44609114	44608209	ID=Msa0813610-mRNA-1:cds;Parent=Msa0813610-mRNA-1
chr5_3	CDS	44610059	44610676	44610059	ID=Msa0813610-mRNA-1:cds;Parent=Msa0813610-mRNA-1
chr5_3	three_prime_UTR	44610677	44610911	44610677	ID=Msa0813610-mRNA-1:three_prime_utr;Parent=Msa0813610-mRNA-1

Gene Sequence

>Msa0813610

ATGAACCAAAACATGTCACCCCTTCTTCTTTTTCCTTTTGCTCTCTTGCTATTCTTCTTCTTGTTCAAAAAACACAAAACATCTAAGAAATCAACAACTCTTCCACCAGGTCCTAAAGGCCTTCCTTTCATTGGAAACTTACACCAACTTGATAGTTCAGCTCTTGGTTTAAACTTCTATGAACTCTCAAAGAAATATGGCCCTATAATCTCCCTTAAACTTGGTTCAAAGCAAACAATAGTTGTTTCATCAGCTAAAATGGCCAAACAAGTAATGAAAACACACGACATCGAATTCTGTAACCGACCCGCTTTAATCAGCCATATGAAATTCTCATATGATGGATTAGACCAGTTTTTTTCACCATATAGAGAATATTGGAGACACACCAAAAAACTTTCCTTCATCCATTTTCTTAGTGTCAAACGTGTCGTAATGTTTTCCTCGGTTAGAAAATACGAGACAACTCAAATGATCAAGAAGATATCAGAACATGCTTCTTCCAACAAAGTTGTTAATTTGCATGAGCTTCTCATGTGTCTAACAAGCGCGGTAGTTTGTAGGACCGCGTTCGGTAGAAGGTTTGAAGATGAAGCAGCTGAACGTAGCATGTTTCATGATTTGCTTAAAGAAGCGCAGGAAATGACGATTTCGTTCTTTTACACGGATTACTTGCCTTTTGTTGGAGGGATTGTTGATAAGTTCACGGGATTGATGAGTCGTCTTGAGAAATTGTTCAAGATTTTGGATGGTTTTTTTCAGAGTGTTGTTGATGAACATCTTGATCCAAATAGGAAGAAGTTGCCTCCACATGAAGATGATGTAATTGATGCTTTGATTGAATTGAAGAATGATCCTTACTGCTCAATGGATCTAAAATCTGAACACATCAAACCCTTGATCATGAATATGTTGCTGGCGGGAACAGATACAATCGCGGCCGCAGTAGTTTGGGCTATGACTGCACTAATGAAGAATCCAAGAGTGATGCAGAAAGTACAAGAAGAGATTCGAAAAGCATATGAAGGGAAGGGTTTTATAGAGGAAGAAGATGTCCAAAAGCTTCCATATTTCAAGGCAGTCATAAAAGAATCAATGAGATTGTACCCGTCATTACCCGTACTTTTACCAAGAGAAACGATGAAAAAGTGCGACATCGAAGGGTATGAAATTCCAGACAAGACATTGGTGTACATTAACGCGTGGGCAATCCATAGAGATCCGGAAGCATGGAAGGATCCAGAAGAGTTTTATCCCGAGAGATTCATAGGCAGTGATATAGATTTAAAAGGACAAGATTTCGAGTTGATTCCGTTTGGTTCAGGGCGAAGAGTTTGTCCTGGCTTAAACATGGCAATTGCTACTGTGGACCTTGTACTTGCTAATCTTCTCTATTTGTTTGATTGGGAAATGCCTGAAGGAGTAAAGTGGGAAGATATAGACATTGATGGTCTTCCAGGACTTGTCCAACATAAGAAAAATCCTCTCTGCCTTGTTGCTAAGAAGAGGATTGAATGTGTTTGA

Protein sequence

>Msa0813610

MNQNMSPLLLFPFALLLFFFLFKKHKTSKKSTTLPPGPKGLPFIGNLHQLDSSALGLNFYELSKKYGPIISLKLGSKQTIVVSSAKMAKQVMKTHDIEFCNRPALISHMKFSYDGLDQFFSPYREYWRHTKKLSFIHFLSVKRVVMFSSVRKYETTQMIKKISEHASSNKVVNLHELLMCLTSAVVCRTAFGRRFEDEAAERSMFHDLLKEAQEMTISFFYTDYLPFVGGIVDKFTGLMSRLEKLFKILDGFFQSVVDEHLDPNRKKLPPHEDDVIDALIELKNDPYCSMDLKSEHIKPLIMNMLLAGTDTIAAAVVWAMTALMKNPRVMQKVQEEIRKAYEGKGFIEEEDVQKLPYFKAVIKESMRLYPSLPVLLPRETMKKCDIEGYEIPDKTLVYINAWAIHRDPEAWKDPEEFYPERFIGSDIDLKGQDFELIPFGSGRRVCPGLNMAIATVDLVLANLLYLFDWEMPEGVKWEDIDIDGLPGLVQHKKNPLCLVAKKRIECV*