Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1269600 | G7K0N9 | 84.615 | 117 | 14 | 1 | 1 | 117 | 6 | 118 | 1.87e-62 | 207 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0682360 | Msa1269600 | 0.807086 | 6.000233e-50 | -8.615850e-47 |
| Msa0745110 | Msa1269600 | 0.881245 | 2.817030e-70 | -8.615850e-47 |
| Msa1269600 | Msa1423990 | 0.808162 | 3.545879e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1269600 | MtrunA17_Chr5g0448311 | 84.615 | 117 | 14 | 1 | 1 | 117 | 6 | 118 | 3.60e-66 | 207 |
| Msa1269600 | MtrunA17_Chr5g0448291 | 76.033 | 121 | 22 | 3 | 8 | 128 | 23 | 136 | 4.68e-56 | 181 |
| Msa1269600 | MtrunA17_Chr5g0448291 | 82.353 | 34 | 6 | 0 | 297 | 330 | 162 | 195 | 2.30e-12 | 65.5 |
| Msa1269600 | MtrunA17_Chr5g0448321 | 52.542 | 59 | 28 | 0 | 18 | 76 | 29 | 87 | 1.31e-15 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGGAGAAGGAGGAGGAGG+AGG | 0.307750 | 8_3:-2831902 | Msa1269600:CDS |
| TTGCACAAGGAATGGCTTAA+AGG | 0.313837 | 8_3:+2832672 | None:intergenic |
| GGAGAAGGAGAAGGAGGAGG+AGG | 0.364992 | 8_3:-2831905 | Msa1269600:CDS |
| GAAGGAGAAGGAGAAGGAGG+AGG | 0.397066 | 8_3:-2831908 | Msa1269600:CDS |
| AGGAGGAGGAAATGCTCTTA+TGG | 0.413357 | 8_3:-2831861 | Msa1269600:CDS |
| GGAGAAGGAGAAGGAGAAGG+AGG | 0.461907 | 8_3:-2831911 | Msa1269600:CDS |
| ATTACGTTATGACCACCATT+TGG | 0.470404 | 8_3:+2832454 | None:intergenic |
| AGAAAAGAGTCTGATGATAG+TGG | 0.476096 | 8_3:-2832398 | Msa1269600:CDS |
| GAAGGAGAAGGAGAAGGAGA+AGG | 0.480096 | 8_3:-2831914 | Msa1269600:CDS |
| TCATGAGTGGTTGATGAAGA+GGG | 0.481340 | 8_3:+2832553 | None:intergenic |
| GAAGAAGAAGGAGAAGGAGA+AGG | 0.489053 | 8_3:-2831920 | Msa1269600:CDS |
| CAAGAAGGATATTGCAGAGA+AGG | 0.490364 | 8_3:+2832608 | None:intergenic |
| CTGATATGTAAAATTCGAAC+CGG | 0.494707 | 8_3:+2832494 | None:intergenic |
| CTAGAGAATTGCACAAGGAA+TGG | 0.511607 | 8_3:+2832664 | None:intergenic |
| AAATGGTGGTCATAACGTAA+TGG | 0.527010 | 8_3:-2832452 | Msa1269600:CDS |
| GAACATGTAACTCTTGTCAA+TGG | 0.544454 | 8_3:+2832630 | None:intergenic |
| TCAGAGATCACAGAACCAAA+TGG | 0.564516 | 8_3:-2832469 | Msa1269600:CDS |
| TTCATGAGTGGTTGATGAAG+AGG | 0.600666 | 8_3:+2832552 | None:intergenic |
| TCGACAGTGAGAAGAGTGAC+CGG | 0.617579 | 8_3:-2832513 | Msa1269600:CDS |
| ATAATGCAATAAAAGCAAGA+AGG | 0.623530 | 8_3:+2832593 | None:intergenic |
| AGAATTAAACAAGAAATGTG+AGG | 0.644539 | 8_3:-2831816 | Msa1269600:CDS |
| GGTCACTCTTCTCACTGTCG+AGG | 0.659355 | 8_3:+2832515 | None:intergenic |
| GATAGTGGAACCAGAAAATG+TGG | 0.659948 | 8_3:-2832383 | Msa1269600:CDS |
| GAGATCACAGAACCAAATGG+TGG | 0.691285 | 8_3:-2832466 | Msa1269600:CDS |
| AGCTGCTAGAGAATTGCACA+AGG | 0.715213 | 8_3:+2832659 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATAATGCAATAAAAGCAAGA+AGG | + | chr8_3:2831891-2831910 | None:intergenic | 25.0% |
| !!! | TTCCACTTTTTCTTTTTCTT+GGG | + | chr8_3:2832064-2832083 | None:intergenic | 25.0% |
| ! | AGAATTAAACAAGAAATGTG+AGG | - | chr8_3:2832665-2832684 | Msa1269600:CDS | 25.0% |
| ! | ATAATGCAATAAAAGCAAGA+AGG | + | chr8_3:2831891-2831910 | None:intergenic | 25.0% |
| !!! | TTCCACTTTTTCTTTTTCTT+GGG | + | chr8_3:2832064-2832083 | None:intergenic | 25.0% |
| ! | AGAATTAAACAAGAAATGTG+AGG | - | chr8_3:2832665-2832684 | Msa1269600:CDS | 25.0% |
| ! | ATGTTCAATGTTTTCATGAG+TGG | + | chr8_3:2831944-2831963 | None:intergenic | 30.0% |
| CTGATATGTAAAATTCGAAC+CGG | + | chr8_3:2831990-2832009 | None:intergenic | 30.0% | |
| ATCCCAAGAAAAAGAAAAAG+TGG | - | chr8_3:2832059-2832078 | Msa1269600:CDS | 30.0% | |
| !!! | CTTCCACTTTTTCTTTTTCT+TGG | + | chr8_3:2832065-2832084 | None:intergenic | 30.0% |
| ! | ATGTTCAATGTTTTCATGAG+TGG | + | chr8_3:2831944-2831963 | None:intergenic | 30.0% |
| CTGATATGTAAAATTCGAAC+CGG | + | chr8_3:2831990-2832009 | None:intergenic | 30.0% | |
| ATCCCAAGAAAAAGAAAAAG+TGG | - | chr8_3:2832059-2832078 | Msa1269600:CDS | 30.0% | |
| !!! | CTTCCACTTTTTCTTTTTCT+TGG | + | chr8_3:2832065-2832084 | None:intergenic | 30.0% |
| GAACATGTAACTCTTGTCAA+TGG | + | chr8_3:2831854-2831873 | None:intergenic | 35.0% | |
| ATTACGTTATGACCACCATT+TGG | + | chr8_3:2832030-2832049 | None:intergenic | 35.0% | |
| AAATGGTGGTCATAACGTAA+TGG | - | chr8_3:2832029-2832048 | Msa1269600:CDS | 35.0% | |
| AGAAAAGAGTCTGATGATAG+TGG | - | chr8_3:2832083-2832102 | Msa1269600:CDS | 35.0% | |
| ! | GCTTCAATAACCACATTTTC+TGG | + | chr8_3:2832111-2832130 | None:intergenic | 35.0% |
| GAAGAAGAAGAAGAAGAAGA+AGG | - | chr8_3:2832141-2832160 | Msa1269600:CDS | 35.0% | |
| GAACATGTAACTCTTGTCAA+TGG | + | chr8_3:2831854-2831873 | None:intergenic | 35.0% | |
| ATTACGTTATGACCACCATT+TGG | + | chr8_3:2832030-2832049 | None:intergenic | 35.0% | |
| AAATGGTGGTCATAACGTAA+TGG | - | chr8_3:2832029-2832048 | Msa1269600:CDS | 35.0% | |
| AGAAAAGAGTCTGATGATAG+TGG | - | chr8_3:2832083-2832102 | Msa1269600:CDS | 35.0% | |
| ! | GCTTCAATAACCACATTTTC+TGG | + | chr8_3:2832111-2832130 | None:intergenic | 35.0% |
| GAAGAAGAAGAAGAAGAAGA+AGG | - | chr8_3:2832141-2832160 | Msa1269600:CDS | 35.0% | |
| TTGCACAAGGAATGGCTTAA+AGG | + | chr8_3:2831812-2831831 | None:intergenic | 40.0% | |
| CTAGAGAATTGCACAAGGAA+TGG | + | chr8_3:2831820-2831839 | None:intergenic | 40.0% | |
| CAAGAAGGATATTGCAGAGA+AGG | + | chr8_3:2831876-2831895 | None:intergenic | 40.0% | |
| !! | TCATGAGTGGTTGATGAAGA+GGG | + | chr8_3:2831931-2831950 | None:intergenic | 40.0% |
| !! | TTCATGAGTGGTTGATGAAG+AGG | + | chr8_3:2831932-2831951 | None:intergenic | 40.0% |
| TCAGAGATCACAGAACCAAA+TGG | - | chr8_3:2832012-2832031 | Msa1269600:CDS | 40.0% | |
| !! | GATAGTGGAACCAGAAAATG+TGG | - | chr8_3:2832098-2832117 | Msa1269600:CDS | 40.0% |
| GAAGAAGAAGAAGAAGGAGA+AGG | - | chr8_3:2832555-2832574 | Msa1269600:CDS | 40.0% | |
| TTGCACAAGGAATGGCTTAA+AGG | + | chr8_3:2831812-2831831 | None:intergenic | 40.0% | |
| CTAGAGAATTGCACAAGGAA+TGG | + | chr8_3:2831820-2831839 | None:intergenic | 40.0% | |
| CAAGAAGGATATTGCAGAGA+AGG | + | chr8_3:2831876-2831895 | None:intergenic | 40.0% | |
| !! | TCATGAGTGGTTGATGAAGA+GGG | + | chr8_3:2831931-2831950 | None:intergenic | 40.0% |
| !! | TTCATGAGTGGTTGATGAAG+AGG | + | chr8_3:2831932-2831951 | None:intergenic | 40.0% |
| TCAGAGATCACAGAACCAAA+TGG | - | chr8_3:2832012-2832031 | Msa1269600:CDS | 40.0% | |
| !! | GATAGTGGAACCAGAAAATG+TGG | - | chr8_3:2832098-2832117 | Msa1269600:CDS | 40.0% |
| GAAGAAGAAGAAGAAGGAGA+AGG | - | chr8_3:2832555-2832574 | Msa1269600:CDS | 40.0% | |
| AGCTGCTAGAGAATTGCACA+AGG | + | chr8_3:2831825-2831844 | None:intergenic | 45.0% | |
| GAGATCACAGAACCAAATGG+TGG | - | chr8_3:2832015-2832034 | Msa1269600:CDS | 45.0% | |
| GAAGAAGAAGGAGAAGGAGA+AGG | - | chr8_3:2832561-2832580 | Msa1269600:CDS | 45.0% | |
| AGGAGGAGGAAATGCTCTTA+TGG | - | chr8_3:2832620-2832639 | Msa1269600:CDS | 45.0% | |
| AGCTGCTAGAGAATTGCACA+AGG | + | chr8_3:2831825-2831844 | None:intergenic | 45.0% | |
| GAGATCACAGAACCAAATGG+TGG | - | chr8_3:2832015-2832034 | Msa1269600:CDS | 45.0% | |
| GAAGAAGAAGGAGAAGGAGA+AGG | - | chr8_3:2832561-2832580 | Msa1269600:CDS | 45.0% | |
| AGGAGGAGGAAATGCTCTTA+TGG | - | chr8_3:2832620-2832639 | Msa1269600:CDS | 45.0% | |
| TCGACAGTGAGAAGAGTGAC+CGG | - | chr8_3:2831968-2831987 | Msa1269600:CDS | 50.0% | |
| GAAGGAGAAGGAGAAGGAGA+AGG | - | chr8_3:2832567-2832586 | Msa1269600:CDS | 50.0% | |
| TCGACAGTGAGAAGAGTGAC+CGG | - | chr8_3:2831968-2831987 | Msa1269600:CDS | 50.0% | |
| GAAGGAGAAGGAGAAGGAGA+AGG | - | chr8_3:2832567-2832586 | Msa1269600:CDS | 50.0% | |
| GGTCACTCTTCTCACTGTCG+AGG | + | chr8_3:2831969-2831988 | None:intergenic | 55.0% | |
| GGAGAAGGAGAAGGAGAAGG+AGG | - | chr8_3:2832570-2832589 | Msa1269600:CDS | 55.0% | |
| GAAGGAGAAGGAGAAGGAGG+AGG | - | chr8_3:2832573-2832592 | Msa1269600:CDS | 55.0% | |
| GGTCACTCTTCTCACTGTCG+AGG | + | chr8_3:2831969-2831988 | None:intergenic | 55.0% | |
| GGAGAAGGAGAAGGAGAAGG+AGG | - | chr8_3:2832570-2832589 | Msa1269600:CDS | 55.0% | |
| GAAGGAGAAGGAGAAGGAGG+AGG | - | chr8_3:2832573-2832592 | Msa1269600:CDS | 55.0% | |
| GGAGAAGGAGAAGGAGGAGG+AGG | - | chr8_3:2832576-2832595 | Msa1269600:CDS | 60.0% | |
| GAAGGAGAAGGAGGAGGAGG+AGG | - | chr8_3:2832579-2832598 | Msa1269600:CDS | 60.0% | |
| GGAGAAGGAGAAGGAGGAGG+AGG | - | chr8_3:2832576-2832595 | Msa1269600:CDS | 60.0% | |
| GAAGGAGAAGGAGGAGGAGG+AGG | - | chr8_3:2832579-2832598 | Msa1269600:CDS | 60.0% | |
| GGAGAAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832582-2832601 | Msa1269600:CDS | 65.0% | |
| GAAGGAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832585-2832604 | Msa1269600:CDS | 65.0% | |
| GGAGAAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832582-2832601 | Msa1269600:CDS | 65.0% | |
| GAAGGAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832585-2832604 | Msa1269600:CDS | 65.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832588-2832607 | Msa1269600:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832588-2832607 | Msa1269600:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832588-2832607 | Msa1269600:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832588-2832607 | Msa1269600:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832588-2832607 | Msa1269600:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832588-2832607 | Msa1269600:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832588-2832607 | Msa1269600:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832588-2832607 | Msa1269600:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832588-2832607 | Msa1269600:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832588-2832607 | Msa1269600:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832588-2832607 | Msa1269600:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832588-2832607 | Msa1269600:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832588-2832607 | Msa1269600:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | - | chr8_3:2832588-2832607 | Msa1269600:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 2831751 | 2832752 | 2831751 | ID=Msa1269600;Name=Msa1269600 |
| chr8_3 | mRNA | 2831751 | 2832752 | 2831751 | ID=Msa1269600-mRNA-1;Parent=Msa1269600;Name=Msa1269600-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|333 |
| chr8_3 | exon | 2831751 | 2832752 | 2831751 | ID=Msa1269600-mRNA-1:exon:954;Parent=Msa1269600-mRNA-1 |
| chr8_3 | CDS | 2831751 | 2832752 | 2831751 | ID=Msa1269600-mRNA-1:cds;Parent=Msa1269600-mRNA-1 |
| Gene Sequence |
| Protein sequence |
