Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0745110 | XP_003618065.1 | 84.615 | 117 | 14 | 1 | 1 | 117 | 6 | 118 | 5.73e-64 | 211 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0745110 | G7K0N9 | 84.615 | 117 | 14 | 1 | 1 | 117 | 6 | 118 | 2.73e-64 | 211 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0023610 | Msa0745110 | 0.801019 | 1.097046e-48 | -8.615850e-47 |
| Msa0639460 | Msa0745110 | 0.845632 | 3.598027e-59 | -8.615850e-47 |
| Msa0675510 | Msa0745110 | 0.805725 | 1.162244e-49 | -8.615850e-47 |
| Msa0682360 | Msa0745110 | 0.857337 | 1.748516e-62 | -8.615850e-47 |
| Msa0745110 | Msa0865390 | 0.814871 | 1.235595e-51 | -8.615850e-47 |
| Msa0745110 | Msa0963310 | 0.806815 | 6.848473e-50 | -8.615850e-47 |
| Msa0745110 | Msa0999350 | 0.816055 | 6.735818e-52 | -8.615850e-47 |
| Msa0745110 | Msa0999400 | 0.822465 | 2.334944e-53 | -8.615850e-47 |
| Msa0745110 | Msa1228190 | 0.800804 | 1.213585e-48 | -8.615850e-47 |
| Msa0745110 | Msa1269600 | 0.881245 | 2.817030e-70 | -8.615850e-47 |
| Msa0745110 | Msa1271260 | 0.804056 | 2.594378e-49 | -8.615850e-47 |
| Msa0745110 | Msa1339440 | 0.818198 | 2.221683e-52 | -8.615850e-47 |
| Msa0745110 | Msa1423990 | 0.815432 | 9.274735e-52 | -8.615850e-47 |
| Msa0546710 | Msa0745110 | 0.835762 | 1.389553e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0745110 | MtrunA17_Chr5g0448311 | 84.615 | 117 | 14 | 1 | 1 | 117 | 6 | 118 | 5.27e-68 | 211 |
| Msa0745110 | MtrunA17_Chr5g0448291 | 59.341 | 182 | 61 | 4 | 10 | 191 | 25 | 193 | 2.29e-57 | 183 |
| Msa0745110 | MtrunA17_Chr5g0448321 | 42.963 | 135 | 67 | 5 | 18 | 150 | 29 | 155 | 6.34e-17 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGGAGAAGGAGGAGGAGG+AGG | 0.307750 | 5_1:+66214839 | Msa0745110:CDS |
| TTGCACAAGGAATGGCTTAA+AGG | 0.313837 | 5_1:-66214055 | None:intergenic |
| GGAGAAGGAGAAGGAGGAGG+AGG | 0.364992 | 5_1:+66214836 | Msa0745110:CDS |
| GAAGGAGAAGGAGAAGGAGG+AGG | 0.397066 | 5_1:+66214833 | Msa0745110:CDS |
| AGGAGGAGGAAATGCTCTTA+TGG | 0.413357 | 5_1:+66214877 | Msa0745110:CDS |
| GGAGAAGGAGAAGGAGAAGG+AGG | 0.461907 | 5_1:+66214830 | Msa0745110:CDS |
| GAAGGAGAAGGAGAAGGAGA+AGG | 0.463599 | 5_1:+66214827 | Msa0745110:CDS |
| ATTACGTTATGACCACCATT+TGG | 0.470404 | 5_1:-66214273 | None:intergenic |
| AGAAAAGAGTCTGATGATAG+TGG | 0.476096 | 5_1:+66214329 | Msa0745110:CDS |
| GAAGGAGAAGGAGAAGGAGA+AGG | 0.480096 | 5_1:+66214821 | Msa0745110:CDS |
| TCATGAGTGGTTGATGAAGA+GGG | 0.481340 | 5_1:-66214174 | None:intergenic |
| GAAGAAGAAGGAGAAGGAGA+AGG | 0.489053 | 5_1:+66214815 | Msa0745110:CDS |
| CAAGAAGGATATTGCAGAGA+AGG | 0.490364 | 5_1:-66214119 | None:intergenic |
| CTGATATGTAAAATTCGAAC+CGG | 0.494707 | 5_1:-66214233 | None:intergenic |
| CTAGAGAATTGCACAAGGAA+TGG | 0.511607 | 5_1:-66214063 | None:intergenic |
| AAATGGTGGTCATAACGTAA+TGG | 0.527010 | 5_1:+66214275 | Msa0745110:CDS |
| GAACATGTAACTCTTGTCAA+TGG | 0.544454 | 5_1:-66214097 | None:intergenic |
| TCAGAGATCACAGAACCAAA+TGG | 0.564516 | 5_1:+66214258 | Msa0745110:CDS |
| TTCATGAGTGGTTGATGAAG+AGG | 0.600666 | 5_1:-66214175 | None:intergenic |
| TCGACAGTGAGAAGAGTGAC+CGG | 0.617579 | 5_1:+66214214 | Msa0745110:CDS |
| ATAATGCAATAAAAGCAAGA+AGG | 0.623530 | 5_1:-66214134 | None:intergenic |
| GGTCACTCTTCTCACTGTCG+AGG | 0.659355 | 5_1:-66214212 | None:intergenic |
| GATAGTGGAACCAGAAAATG+TGG | 0.659948 | 5_1:+66214344 | Msa0745110:CDS |
| GAGATCACAGAACCAAATGG+TGG | 0.691285 | 5_1:+66214261 | Msa0745110:CDS |
| AGCTGCTAGAGAATTGCACA+AGG | 0.715213 | 5_1:-66214068 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATAATGCAATAAAAGCAAGA+AGG | - | chr5_1:66214137-66214156 | None:intergenic | 25.0% |
| !!! | TTCCACTTTTTCTTTTTCTT+GGG | - | chr5_1:66214310-66214329 | None:intergenic | 25.0% |
| ! | ATAATGCAATAAAAGCAAGA+AGG | - | chr5_1:66214137-66214156 | None:intergenic | 25.0% |
| !!! | TTCCACTTTTTCTTTTTCTT+GGG | - | chr5_1:66214310-66214329 | None:intergenic | 25.0% |
| ! | ATGTTCAATGTTTTCATGAG+TGG | - | chr5_1:66214190-66214209 | None:intergenic | 30.0% |
| CTGATATGTAAAATTCGAAC+CGG | - | chr5_1:66214236-66214255 | None:intergenic | 30.0% | |
| ATCCCAAGAAAAAGAAAAAG+TGG | + | chr5_1:66214305-66214324 | Msa0745110:CDS | 30.0% | |
| !!! | CTTCCACTTTTTCTTTTTCT+TGG | - | chr5_1:66214311-66214330 | None:intergenic | 30.0% |
| ! | ATGTTCAATGTTTTCATGAG+TGG | - | chr5_1:66214190-66214209 | None:intergenic | 30.0% |
| CTGATATGTAAAATTCGAAC+CGG | - | chr5_1:66214236-66214255 | None:intergenic | 30.0% | |
| ATCCCAAGAAAAAGAAAAAG+TGG | + | chr5_1:66214305-66214324 | Msa0745110:CDS | 30.0% | |
| !!! | CTTCCACTTTTTCTTTTTCT+TGG | - | chr5_1:66214311-66214330 | None:intergenic | 30.0% |
| GAACATGTAACTCTTGTCAA+TGG | - | chr5_1:66214100-66214119 | None:intergenic | 35.0% | |
| ATTACGTTATGACCACCATT+TGG | - | chr5_1:66214276-66214295 | None:intergenic | 35.0% | |
| AAATGGTGGTCATAACGTAA+TGG | + | chr5_1:66214275-66214294 | Msa0745110:CDS | 35.0% | |
| AGAAAAGAGTCTGATGATAG+TGG | + | chr5_1:66214329-66214348 | Msa0745110:CDS | 35.0% | |
| ! | GCTTCAATAACCACATTTTC+TGG | - | chr5_1:66214357-66214376 | None:intergenic | 35.0% |
| GAAGAAGAAGAAGAAGAAGA+AGG | + | chr5_1:66214387-66214406 | Msa0745110:CDS | 35.0% | |
| GAACATGTAACTCTTGTCAA+TGG | - | chr5_1:66214100-66214119 | None:intergenic | 35.0% | |
| ATTACGTTATGACCACCATT+TGG | - | chr5_1:66214276-66214295 | None:intergenic | 35.0% | |
| AAATGGTGGTCATAACGTAA+TGG | + | chr5_1:66214275-66214294 | Msa0745110:CDS | 35.0% | |
| AGAAAAGAGTCTGATGATAG+TGG | + | chr5_1:66214329-66214348 | Msa0745110:CDS | 35.0% | |
| ! | GCTTCAATAACCACATTTTC+TGG | - | chr5_1:66214357-66214376 | None:intergenic | 35.0% |
| GAAGAAGAAGAAGAAGAAGA+AGG | + | chr5_1:66214387-66214406 | Msa0745110:CDS | 35.0% | |
| TTGCACAAGGAATGGCTTAA+AGG | - | chr5_1:66214058-66214077 | None:intergenic | 40.0% | |
| CTAGAGAATTGCACAAGGAA+TGG | - | chr5_1:66214066-66214085 | None:intergenic | 40.0% | |
| CAAGAAGGATATTGCAGAGA+AGG | - | chr5_1:66214122-66214141 | None:intergenic | 40.0% | |
| !! | TCATGAGTGGTTGATGAAGA+GGG | - | chr5_1:66214177-66214196 | None:intergenic | 40.0% |
| !! | TTCATGAGTGGTTGATGAAG+AGG | - | chr5_1:66214178-66214197 | None:intergenic | 40.0% |
| TCAGAGATCACAGAACCAAA+TGG | + | chr5_1:66214258-66214277 | Msa0745110:CDS | 40.0% | |
| !! | GATAGTGGAACCAGAAAATG+TGG | + | chr5_1:66214344-66214363 | Msa0745110:CDS | 40.0% |
| GAAGAAGAAGAAGAAGGAGA+AGG | + | chr5_1:66214809-66214828 | Msa0745110:CDS | 40.0% | |
| TTGCACAAGGAATGGCTTAA+AGG | - | chr5_1:66214058-66214077 | None:intergenic | 40.0% | |
| CTAGAGAATTGCACAAGGAA+TGG | - | chr5_1:66214066-66214085 | None:intergenic | 40.0% | |
| CAAGAAGGATATTGCAGAGA+AGG | - | chr5_1:66214122-66214141 | None:intergenic | 40.0% | |
| !! | TCATGAGTGGTTGATGAAGA+GGG | - | chr5_1:66214177-66214196 | None:intergenic | 40.0% |
| !! | TTCATGAGTGGTTGATGAAG+AGG | - | chr5_1:66214178-66214197 | None:intergenic | 40.0% |
| TCAGAGATCACAGAACCAAA+TGG | + | chr5_1:66214258-66214277 | Msa0745110:CDS | 40.0% | |
| !! | GATAGTGGAACCAGAAAATG+TGG | + | chr5_1:66214344-66214363 | Msa0745110:CDS | 40.0% |
| GAAGAAGAAGAAGAAGGAGA+AGG | + | chr5_1:66214809-66214828 | Msa0745110:CDS | 40.0% | |
| AGCTGCTAGAGAATTGCACA+AGG | - | chr5_1:66214071-66214090 | None:intergenic | 45.0% | |
| GAGATCACAGAACCAAATGG+TGG | + | chr5_1:66214261-66214280 | Msa0745110:CDS | 45.0% | |
| GAAGAAGAAGGAGAAGGAGA+AGG | + | chr5_1:66214815-66214834 | Msa0745110:CDS | 45.0% | |
| AGGAGGAGGAAATGCTCTTA+TGG | + | chr5_1:66214877-66214896 | Msa0745110:CDS | 45.0% | |
| AGCTGCTAGAGAATTGCACA+AGG | - | chr5_1:66214071-66214090 | None:intergenic | 45.0% | |
| GAGATCACAGAACCAAATGG+TGG | + | chr5_1:66214261-66214280 | Msa0745110:CDS | 45.0% | |
| GAAGAAGAAGGAGAAGGAGA+AGG | + | chr5_1:66214815-66214834 | Msa0745110:CDS | 45.0% | |
| AGGAGGAGGAAATGCTCTTA+TGG | + | chr5_1:66214877-66214896 | Msa0745110:CDS | 45.0% | |
| TCGACAGTGAGAAGAGTGAC+CGG | + | chr5_1:66214214-66214233 | Msa0745110:CDS | 50.0% | |
| GAAGGAGAAGGAGAAGGAGA+AGG | + | chr5_1:66214821-66214840 | Msa0745110:CDS | 50.0% | |
| GAAGGAGAAGGAGAAGGAGA+AGG | + | chr5_1:66214821-66214840 | Msa0745110:CDS | 50.0% | |
| TCGACAGTGAGAAGAGTGAC+CGG | + | chr5_1:66214214-66214233 | Msa0745110:CDS | 50.0% | |
| GAAGGAGAAGGAGAAGGAGA+AGG | + | chr5_1:66214821-66214840 | Msa0745110:CDS | 50.0% | |
| GAAGGAGAAGGAGAAGGAGA+AGG | + | chr5_1:66214821-66214840 | Msa0745110:CDS | 50.0% | |
| GGTCACTCTTCTCACTGTCG+AGG | - | chr5_1:66214215-66214234 | None:intergenic | 55.0% | |
| GGAGAAGGAGAAGGAGAAGG+AGG | + | chr5_1:66214824-66214843 | Msa0745110:CDS | 55.0% | |
| GAAGGAGAAGGAGAAGGAGG+AGG | + | chr5_1:66214833-66214852 | Msa0745110:CDS | 55.0% | |
| GGTCACTCTTCTCACTGTCG+AGG | - | chr5_1:66214215-66214234 | None:intergenic | 55.0% | |
| GGAGAAGGAGAAGGAGAAGG+AGG | + | chr5_1:66214824-66214843 | Msa0745110:CDS | 55.0% | |
| GAAGGAGAAGGAGAAGGAGG+AGG | + | chr5_1:66214833-66214852 | Msa0745110:CDS | 55.0% | |
| GGAGAAGGAGAAGGAGGAGG+AGG | + | chr5_1:66214836-66214855 | Msa0745110:CDS | 60.0% | |
| GAAGGAGAAGGAGGAGGAGG+AGG | + | chr5_1:66214839-66214858 | Msa0745110:CDS | 60.0% | |
| GGAGAAGGAGAAGGAGGAGG+AGG | + | chr5_1:66214836-66214855 | Msa0745110:CDS | 60.0% | |
| GAAGGAGAAGGAGGAGGAGG+AGG | + | chr5_1:66214839-66214858 | Msa0745110:CDS | 60.0% | |
| GGAGAAGGAGGAGGAGGAGG+AGG | + | chr5_1:66214842-66214861 | Msa0745110:CDS | 65.0% | |
| GAAGGAGGAGGAGGAGGAGG+AGG | + | chr5_1:66214845-66214864 | Msa0745110:CDS | 65.0% | |
| GGAGAAGGAGGAGGAGGAGG+AGG | + | chr5_1:66214842-66214861 | Msa0745110:CDS | 65.0% | |
| GAAGGAGGAGGAGGAGGAGG+AGG | + | chr5_1:66214845-66214864 | Msa0745110:CDS | 65.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | + | chr5_1:66214848-66214867 | Msa0745110:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | + | chr5_1:66214848-66214867 | Msa0745110:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | + | chr5_1:66214848-66214867 | Msa0745110:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | + | chr5_1:66214848-66214867 | Msa0745110:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | + | chr5_1:66214848-66214867 | Msa0745110:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | + | chr5_1:66214848-66214867 | Msa0745110:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | + | chr5_1:66214848-66214867 | Msa0745110:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | + | chr5_1:66214848-66214867 | Msa0745110:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | + | chr5_1:66214848-66214867 | Msa0745110:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | + | chr5_1:66214848-66214867 | Msa0745110:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | + | chr5_1:66214848-66214867 | Msa0745110:CDS | 70.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | + | chr5_1:66214848-66214867 | Msa0745110:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 66213997 | 66214902 | 66213997 | ID=Msa0745110;Name=Msa0745110 |
| chr5_1 | mRNA | 66213997 | 66214902 | 66213997 | ID=Msa0745110-mRNA-1;Parent=Msa0745110;Name=Msa0745110-mRNA-1;_AED=0.47;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|301 |
| chr5_1 | exon | 66213997 | 66214902 | 66213997 | ID=Msa0745110-mRNA-1:exon:19710;Parent=Msa0745110-mRNA-1 |
| chr5_1 | CDS | 66213997 | 66214902 | 66213997 | ID=Msa0745110-mRNA-1:cds;Parent=Msa0745110-mRNA-1 |
| Gene Sequence |
| Protein sequence |
