AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1269920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1269920 MtrunA17_Chr1g0151801 73.548 155 8 1 130 251 69 223 1.13e-71 228
Msa1269920 MtrunA17_Chr3g0135491 63.871 155 23 1 130 251 60 214 5.82e-62 202
Msa1269920 MtrunA17_Chr4g0056561 54.839 155 37 1 130 251 70 224 1.95e-49 170
Msa1269920 MtrunA17_Chr7g0248651 47.742 155 48 1 130 251 58 212 3.62e-42 150
Msa1269920 MtrunA17_Chr7g0246051 47.712 153 47 1 132 251 59 211 3.92e-42 145
Msa1269920 MtrunA17_Chr7g0226421 44.094 127 33 2 123 211 33 159 9.28e-31 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1269920 AT4G32940.1 63.226 155 24 1 130 251 70 224 1.89e-62 204
Msa1269920 AT2G25940.1 63.226 155 24 1 130 251 55 209 8.60e-62 202
Msa1269920 AT1G62710.1 56.129 155 35 1 130 251 62 216 1.18e-52 179
Msa1269920 AT3G20210.1 51.613 155 42 1 130 251 57 211 4.53e-49 169

Find 59 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGATGGAGAAAGATTCTT+TGG 0.294170 8_3:-3262093 None:intergenic
TGGAATCTGGAAGAGATATA+AGG 0.338125 8_3:+3259771 Msa1269920:CDS
CATGGAGGTCCAGGGGTTCT+TGG 0.340017 8_3:+3261042 Msa1269920:CDS
TATCTGGAGGCATGTGAATC+TGG 0.353249 8_3:+3261892 Msa1269920:CDS
AGAAATTGTAACCTTTGAAT+CGG 0.388325 8_3:-3259928 None:intergenic
TGAGCTTTGACGGTGAGCTT+TGG 0.392923 8_3:-3259564 None:intergenic
TTGCGATAGGGAAAGGGATA+AGG 0.400361 8_3:-3259480 None:intergenic
GTGATGGAGAGGGTGGAATC+TGG 0.407879 8_3:+3259758 Msa1269920:intron
TTTGTATACTACACTGATCA+TGG 0.415438 8_3:+3261024 Msa1269920:CDS
AGGAGCGGAATCGTTGATTT+CGG 0.416025 8_3:-3259859 None:intergenic
TTAATTATGACTCCAGGCCT+TGG 0.438134 8_3:-3260626 None:intergenic
GTCTCTTCAGGTATGCCTGT+TGG 0.440122 8_3:+3261589 Msa1269920:intron
TACACTGATCATGGAGGTCC+AGG 0.458300 8_3:+3261033 Msa1269920:CDS
GGTGAGCTTTGGAGGTTTCA+CGG 0.458353 8_3:-3259553 None:intergenic
GCTTTGACGGTGAGCTTTGG+AGG 0.460896 8_3:-3259561 None:intergenic
GTCATGCGTATCAATTATTG+AGG 0.462318 8_3:+3260534 Msa1269920:CDS
GGAATCTGGAAGAGATATAA+GGG 0.473052 8_3:+3259772 Msa1269920:CDS
GCATACAAGTAAGGACCAAC+AGG 0.477482 8_3:-3261604 None:intergenic
GAAATTGTAACCTTTGAATC+GGG 0.482521 8_3:-3259927 None:intergenic
AGTGGGAAAGTTGTGGATAG+TGG 0.492444 8_3:+3260985 Msa1269920:CDS
TCATAAACATCACCACCATC+AGG 0.496461 8_3:-3260653 None:intergenic
GCTTTGACGGTGAGCTTTGA+CGG 0.498645 8_3:-3259574 None:intergenic
AGATCAGATGCATACAAGTA+AGG 0.499897 8_3:-3261613 None:intergenic
GTAACCTTTGAATCGGGTGA+TGG 0.501298 8_3:-3259921 None:intergenic
GCTTGGACGGTGAGCTTTGA+CGG 0.502837 8_3:-3259587 None:intergenic
GAATCTTTCTCCATCTGAAA+CGG 0.505300 8_3:+3262098 Msa1269920:CDS
AGAGCGATGTAGCAACGGTA+AGG 0.507375 8_3:-3259813 None:intergenic
AGGAAACCACTGATTGAACT+TGG 0.513981 8_3:-3259638 None:intergenic
GGTTTGTTAATTATGACTCC+AGG 0.517005 8_3:-3260632 None:intergenic
GGAGATTCACGATTGGAGCT+TGG 0.532522 8_3:-3259604 None:intergenic
TCAGCTCTTACAGGTGGGAG+TGG 0.533587 8_3:+3260967 Msa1269920:intron
TAACCTTTGAATCGGGTGAT+GGG 0.534964 8_3:-3259920 None:intergenic
TTTCCCATCACCCGATTCAA+AGG 0.536125 8_3:+3259917 Msa1269920:CDS
AGGTGGGAGTGGGAAAGTTG+TGG 0.538762 8_3:+3260978 Msa1269920:intron
AATGTAGAGAATCCAAGGCC+TGG 0.541898 8_3:+3260614 Msa1269920:CDS
TCTCTATCCACCGTTTCAGA+TGG 0.558580 8_3:-3262108 None:intergenic
TTGAACTTGGACGGTGAGGT+TGG 0.560420 8_3:-3259625 None:intergenic
AGGCCTTGGATTCTCTACAT+TGG 0.562654 8_3:-3260612 None:intergenic
GCGTATCAATTATTGAGGAA+TGG 0.567662 8_3:+3260539 Msa1269920:CDS
GTCATAATTAACAAACCTGA+TGG 0.573212 8_3:+3260638 Msa1269920:CDS
AATTACTCACCAAGAACCCC+TGG 0.573730 8_3:-3261051 None:intergenic
ATTCACGATTGGAGCTTGGA+CGG 0.575479 8_3:-3259600 None:intergenic
TCTTTCTCCATCTGAAACGG+TGG 0.582359 8_3:+3262101 Msa1269920:CDS
CTTCCAATGTAGAGAATCCA+AGG 0.584961 8_3:+3260609 Msa1269920:CDS
TGAGGTTGGAGATTCACGAT+TGG 0.587323 8_3:-3259611 None:intergenic
TCTGAAACGGTGGATAGAGA+TGG 0.587933 8_3:+3262111 Msa1269920:CDS
GAGCGATGTAGCAACGGTAA+GGG 0.592486 8_3:-3259812 None:intergenic
ACACTGATCATGGAGGTCCA+GGG 0.595033 8_3:+3261034 Msa1269920:CDS
CAGCTCTTACAGGTGGGAGT+GGG 0.602741 8_3:+3260968 Msa1269920:intron
AACCACTGATTGAACTTGGA+CGG 0.602863 8_3:-3259634 None:intergenic
TATCAATTATTGAGGAATGG+TGG 0.612607 8_3:+3260542 Msa1269920:CDS
CACTGATCATGGAGGTCCAG+GGG 0.620699 8_3:+3261035 Msa1269920:CDS
CACCGTCCAAGTTCAATCAG+TGG 0.621289 8_3:+3259632 Msa1269920:CDS
GATCAAGAGCGATGTAGCAA+CGG 0.624037 8_3:-3259818 None:intergenic
TTAACAGCTTGAAGAGACGT+AGG 0.645425 8_3:-3259879 None:intergenic
ATAATTAACAAACCTGATGG+TGG 0.650511 8_3:+3260641 Msa1269920:CDS
AGCTTGAAGAGACGTAGGAG+CGG 0.657960 8_3:-3259874 None:intergenic
CTGATTGAACTTGGACGGTG+AGG 0.709832 8_3:-3259629 None:intergenic
GTATACTACACTGATCATGG+AGG 0.754047 8_3:+3261027 Msa1269920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATACAAAAAGAAATTA+AGG - chr8_3:3260456-3260475 None:intergenic 10.0%
!!! TCTATTTTTTAAAATACTAT+CGG + chr8_3:3261202-3261221 Msa1269920:intron 10.0%
!!! TATTTAATTATAACTGTTTT+TGG - chr8_3:3261474-3261493 None:intergenic 10.0%
!!! ATTTTTTTTGAACAACAAAT+GGG - chr8_3:3259710-3259729 None:intergenic 15.0%
!!! AATTTTTTTTGAACAACAAA+TGG - chr8_3:3259711-3259730 None:intergenic 15.0%
!! TAATAGATAACAAGTAATAT+TGG - chr8_3:3260083-3260102 None:intergenic 15.0%
!!! ATATGATCAATAACATATTT+TGG + chr8_3:3260430-3260449 Msa1269920:intron 15.0%
!! TATACAAAAATATGATCATT+GGG - chr8_3:3261012-3261031 None:intergenic 15.0%
!!! AAAAGCAATTGTTATTTTAA+TGG + chr8_3:3262053-3262072 Msa1269920:intron 15.0%
!!! TTTATCATTGTTGTTATTGT+AGG + chr8_3:3259994-3260013 Msa1269920:intron 20.0%
!!! AATATTAGTTTCTTGTTTAG+TGG - chr8_3:3260145-3260164 None:intergenic 20.0%
!!! ACTTATAATTTAAAGTCAAC+TGG + chr8_3:3260191-3260210 Msa1269920:intron 20.0%
!! AATGAAAATAGTTGAATGTA+TGG + chr8_3:3260317-3260336 Msa1269920:intron 20.0%
!! ATGAAAATAGTTGAATGTAT+GGG + chr8_3:3260318-3260337 Msa1269920:intron 20.0%
!! TTGAAAAACAATTTATGTAC+AGG + chr8_3:3260348-3260367 Msa1269920:intron 20.0%
!! TGAAAAACAATTTATGTACA+GGG + chr8_3:3260349-3260368 Msa1269920:intron 20.0%
!! TCAATTTCTCAAATTATTAC+TGG + chr8_3:3260793-3260812 Msa1269920:intron 20.0%
!! GTATACAAAAATATGATCAT+TGG - chr8_3:3261013-3261032 None:intergenic 20.0%
!!! AAACTTTTTTATGAAAGACA+TGG + chr8_3:3261091-3261110 Msa1269920:intron 20.0%
!! TTGAATATTATCACATGTAT+CGG - chr8_3:3261177-3261196 None:intergenic 20.0%
!!! TTTTTTGACTTATGTAATTG+TGG + chr8_3:3261858-3261877 Msa1269920:intron 20.0%
! AGAAATTGTAACCTTTGAAT+CGG - chr8_3:3259931-3259950 None:intergenic 25.0%
!! ATTGTTGTTATTGTAGGAAT+CGG + chr8_3:3260000-3260019 Msa1269920:intron 25.0%
! TGAAAATAGTTGAATGTATG+GGG + chr8_3:3260319-3260338 Msa1269920:intron 25.0%
! ATTTATGTACAGGGATTAAA+TGG + chr8_3:3260358-3260377 Msa1269920:intron 25.0%
! AAATGGAAACTGTTTAATAG+AGG + chr8_3:3260375-3260394 Msa1269920:intron 25.0%
!!! ACTAATATTGACTTTACCTT+TGG - chr8_3:3260686-3260705 None:intergenic 25.0%
! AGCTATTGAATGTAACAATA+TGG - chr8_3:3260773-3260792 None:intergenic 25.0%
! TGTTTGTATGTGTTTACAAA+TGG + chr8_3:3261273-3261292 Msa1269920:intron 25.0%
!!! TATTCCTTGCTATTCTTATT+TGG + chr8_3:3261567-3261586 Msa1269920:intron 25.0%
! ACAATCAAAACATACAACAT+GGG - chr8_3:3262017-3262036 None:intergenic 25.0%
! TACAATCAAAACATACAACA+TGG - chr8_3:3262018-3262037 None:intergenic 25.0%
AAAGTGAAGAAGGAAAAATG+TGG - chr8_3:3259513-3259532 None:intergenic 30.0%
!! TTTGAACAACAAATGGGTTA+GGG - chr8_3:3259704-3259723 None:intergenic 30.0%
!! TTTTGAACAACAAATGGGTT+AGG - chr8_3:3259705-3259724 None:intergenic 30.0%
GAAATTGTAACCTTTGAATC+GGG - chr8_3:3259930-3259949 None:intergenic 30.0%
AGGTAATGTTGAAATTCAGA+AGG + chr8_3:3260395-3260414 Msa1269920:intron 30.0%
TATCAATTATTGAGGAATGG+TGG + chr8_3:3260542-3260561 Msa1269920:CDS 30.0%
GTCATAATTAACAAACCTGA+TGG + chr8_3:3260638-3260657 Msa1269920:CDS 30.0%
ATAATTAACAAACCTGATGG+TGG + chr8_3:3260641-3260660 Msa1269920:CDS 30.0%
AATAAAATAGACACGAACAC+TGG - chr8_3:3260841-3260860 None:intergenic 30.0%
!! TAAGAGCTTTTTCTTATGAC+AGG + chr8_3:3260885-3260904 Msa1269920:intron 30.0%
!! AATTTCTATGCTGCTTTACT+TGG + chr8_3:3260937-3260956 Msa1269920:intron 30.0%
TTTGTATACTACACTGATCA+TGG + chr8_3:3261024-3261043 Msa1269920:CDS 30.0%
! CTACTTTGAGGAAATCATAT+GGG - chr8_3:3261414-3261433 None:intergenic 30.0%
! TCTACTTTGAGGAAATCATA+TGG - chr8_3:3261415-3261434 None:intergenic 30.0%
AGAACACAAATTCTACTTTG+AGG - chr8_3:3261426-3261445 None:intergenic 30.0%
AAAGTAGAATTTGTGTTCTG+TGG + chr8_3:3261427-3261446 Msa1269920:intron 30.0%
! TATTCTTATTTGGTCTCTTC+AGG + chr8_3:3261577-3261596 Msa1269920:intron 30.0%
TTGAAGAAAAAACATGCTTC+TGG + chr8_3:3261646-3261665 Msa1269920:CDS 30.0%
CTTCCATGTTTGATTATTTC+AGG + chr8_3:3261751-3261770 Msa1269920:intron 30.0%
TAGCCTGAAATAATCAAACA+TGG - chr8_3:3261757-3261776 None:intergenic 30.0%
!! TTAGTTTGTCTTCAACGAAA+AGG - chr8_3:3261813-3261832 None:intergenic 30.0%
CAAACTAAGCTAATCAAAAC+CGG + chr8_3:3261825-3261844 Msa1269920:intron 30.0%
GTCAAAAAAATTTAGTGTGC+CGG - chr8_3:3261847-3261866 None:intergenic 30.0%
ACTGAGTGAAAAAGTGAAGA+AGG - chr8_3:3259523-3259542 None:intergenic 35.0%
GTTAGGGTTTCACGATTTAA+AGG - chr8_3:3259688-3259707 None:intergenic 35.0%
! CACTGATTGAAAAGAGGTAA+GGG - chr8_3:3259741-3259760 None:intergenic 35.0%
! TCACTGATTGAAAAGAGGTA+AGG - chr8_3:3259742-3259761 None:intergenic 35.0%
! TACCTCTTTTCAATCAGTGA+TGG + chr8_3:3259742-3259761 Msa1269920:intron 35.0%
!! TTTTCAATCAGTGATGGAGA+GGG + chr8_3:3259748-3259767 Msa1269920:intron 35.0%
TGGAATCTGGAAGAGATATA+AGG + chr8_3:3259771-3259790 Msa1269920:CDS 35.0%
GGAATCTGGAAGAGATATAA+GGG + chr8_3:3259772-3259791 Msa1269920:CDS 35.0%
!! GAGTTGATAATAGCTGACAT+CGG - chr8_3:3260226-3260245 None:intergenic 35.0%
! ACTGCTATACAGCAACTTTT+TGG - chr8_3:3260288-3260307 None:intergenic 35.0%
!! TTTTTGTTGCTTGCTTCATC+AGG + chr8_3:3260502-3260521 Msa1269920:intron 35.0%
GTCATGCGTATCAATTATTG+AGG + chr8_3:3260534-3260553 Msa1269920:CDS 35.0%
GCGTATCAATTATTGAGGAA+TGG + chr8_3:3260539-3260558 Msa1269920:CDS 35.0%
!! GGTTTGTTAATTATGACTCC+AGG - chr8_3:3260635-3260654 None:intergenic 35.0%
TGTTTATGAAGGAGTTCCAA+AGG + chr8_3:3260667-3260686 Msa1269920:intron 35.0%
TTCTTATGACAGGATTACAC+TGG + chr8_3:3260895-3260914 Msa1269920:intron 35.0%
GGAAATAAATCAGCTCTTAC+AGG + chr8_3:3260958-3260977 Msa1269920:intron 35.0%
AATAAATCAGCTCTTACAGG+TGG + chr8_3:3260961-3260980 Msa1269920:intron 35.0%
ATAAATCAGCTCTTACAGGT+GGG + chr8_3:3260962-3260981 Msa1269920:intron 35.0%
GAGACCAAATAAGAATAGCA+AGG - chr8_3:3261574-3261593 None:intergenic 35.0%
AGATCAGATGCATACAAGTA+AGG - chr8_3:3261616-3261635 None:intergenic 35.0%
AAGAACTACACAGAACAAAC+GGG - chr8_3:3261734-3261753 None:intergenic 35.0%
GAAGAACTACACAGAACAAA+CGG - chr8_3:3261735-3261754 None:intergenic 35.0%
GACTTATGTAATTGTGGTAC+AGG + chr8_3:3261864-3261883 Msa1269920:intron 35.0%
! TGTGGTACAGGTATTTTATC+TGG + chr8_3:3261876-3261895 Msa1269920:intron 35.0%
TACTCTTTCTGCTTAACTCT+TGG + chr8_3:3261984-3262003 Msa1269920:intron 35.0%
ACTCTTTCTGCTTAACTCTT+GGG + chr8_3:3261985-3262004 Msa1269920:intron 35.0%
TCAGATGGAGAAAGATTCTT+TGG - chr8_3:3262096-3262115 None:intergenic 35.0%
GAATCTTTCTCCATCTGAAA+CGG + chr8_3:3262098-3262117 Msa1269920:CDS 35.0%
! TCTGTTTTGCGATAGGGAAA+GGG - chr8_3:3259489-3259508 None:intergenic 40.0%
! ATCTGTTTTGCGATAGGGAA+AGG - chr8_3:3259490-3259509 None:intergenic 40.0%
! TGTGGATCTGTTTTGCGATA+GGG - chr8_3:3259495-3259514 None:intergenic 40.0%
! ATGTGGATCTGTTTTGCGAT+AGG - chr8_3:3259496-3259515 None:intergenic 40.0%
! AACCACTGATTGAACTTGGA+CGG - chr8_3:3259637-3259656 None:intergenic 40.0%
! AGGAAACCACTGATTGAACT+TGG - chr8_3:3259641-3259660 None:intergenic 40.0%
! CTCCATCACTGATTGAAAAG+AGG - chr8_3:3259747-3259766 None:intergenic 40.0%
!! CTTTTCAATCAGTGATGGAG+AGG + chr8_3:3259747-3259766 Msa1269920:intron 40.0%
TTAACAGCTTGAAGAGACGT+AGG - chr8_3:3259882-3259901 None:intergenic 40.0%
TAACCTTTGAATCGGGTGAT+GGG - chr8_3:3259923-3259942 None:intergenic 40.0%
GCGATCGCGAGTATTAAAAA+TGG + chr8_3:3260022-3260041 Msa1269920:intron 40.0%
CTTCCAATGTAGAGAATCCA+AGG + chr8_3:3260609-3260628 Msa1269920:CDS 40.0%
TTAATTATGACTCCAGGCCT+TGG - chr8_3:3260629-3260648 None:intergenic 40.0%
TCATAAACATCACCACCATC+AGG - chr8_3:3260656-3260675 None:intergenic 40.0%
! GATGGTGGTGATGTTTATGA+AGG + chr8_3:3260656-3260675 Msa1269920:intron 40.0%
ACGTCTTTAGTGTGAAGTGT+CGG - chr8_3:3260724-3260743 None:intergenic 40.0%
GTATACTACACTGATCATGG+AGG + chr8_3:3261027-3261046 Msa1269920:CDS 40.0%
!! AAAAGCCTAAGCCTTGTTTG+AGG + chr8_3:3261297-3261316 Msa1269920:intron 40.0%
AATAGCCTCAAACAAGGCTT+AGG - chr8_3:3261305-3261324 None:intergenic 40.0%
TTGAGGAAATCATATGGGAG+AGG - chr8_3:3261409-3261428 None:intergenic 40.0%
AGAACTACACAGAACAAACG+GGG - chr8_3:3261733-3261752 None:intergenic 40.0%
! GGTACAGGTATTTTATCTGG+AGG + chr8_3:3261879-3261898 Msa1269920:intron 40.0%
CTCTTTCTGCTTAACTCTTG+GGG + chr8_3:3261986-3262005 Msa1269920:intron 40.0%
TTGCGATAGGGAAAGGGATA+AGG - chr8_3:3259483-3259502 None:intergenic 45.0%
ATTCACGATTGGAGCTTGGA+CGG - chr8_3:3259603-3259622 None:intergenic 45.0%
TGAGGTTGGAGATTCACGAT+TGG - chr8_3:3259614-3259633 None:intergenic 45.0%
GTTCAATCAGTGGTTTCCTC+TGG + chr8_3:3259642-3259661 Msa1269920:intron 45.0%
GATCAAGAGCGATGTAGCAA+CGG - chr8_3:3259821-3259840 None:intergenic 45.0%
!! AGGAGCGGAATCGTTGATTT+CGG - chr8_3:3259862-3259881 None:intergenic 45.0%
TTTCCCATCACCCGATTCAA+AGG + chr8_3:3259917-3259936 Msa1269920:CDS 45.0%
GTAACCTTTGAATCGGGTGA+TGG - chr8_3:3259924-3259943 None:intergenic 45.0%
AGGCCTTGGATTCTCTACAT+TGG - chr8_3:3260615-3260634 None:intergenic 45.0%
AATGTAGAGAATCCAAGGCC+TGG + chr8_3:3260614-3260633 Msa1269920:CDS 45.0%
AGTGGGAAAGTTGTGGATAG+TGG + chr8_3:3260985-3261004 Msa1269920:CDS 45.0%
AATTACTCACCAAGAACCCC+TGG - chr8_3:3261054-3261073 None:intergenic 45.0%
GTCAGCAATAGCCTCAAACA+AGG - chr8_3:3261311-3261330 None:intergenic 45.0%
GCATACAAGTAAGGACCAAC+AGG - chr8_3:3261607-3261626 None:intergenic 45.0%
! TATCTGGAGGCATGTGAATC+TGG + chr8_3:3261892-3261911 Msa1269920:CDS 45.0%
! TCTTTCTCCATCTGAAACGG+TGG + chr8_3:3262101-3262120 Msa1269920:CDS 45.0%
TCTCTATCCACCGTTTCAGA+TGG - chr8_3:3262111-3262130 None:intergenic 45.0%
! TCTGAAACGGTGGATAGAGA+TGG + chr8_3:3262111-3262130 Msa1269920:CDS 45.0%
!! TTAAAAAAAAGTAATATTAT+GGG - chr8_3:3260052-3260071 None:intergenic 5.0%
!! ATTAAAAAAAAGTAATATTA+TGG - chr8_3:3260053-3260072 None:intergenic 5.0%
!! ATATATATTTATTAAAGTTA+TGG + chr8_3:3261947-3261966 Msa1269920:intron 5.0%
!! GGTGAGCTTTGGAGGTTTCA+CGG - chr8_3:3259556-3259575 None:intergenic 50.0%
! TGAGCTTTGACGGTGAGCTT+TGG - chr8_3:3259567-3259586 None:intergenic 50.0%
! GCTTTGACGGTGAGCTTTGA+CGG - chr8_3:3259577-3259596 None:intergenic 50.0%
GGAGATTCACGATTGGAGCT+TGG - chr8_3:3259607-3259626 None:intergenic 50.0%
TTGAACTTGGACGGTGAGGT+TGG - chr8_3:3259628-3259647 None:intergenic 50.0%
CTGATTGAACTTGGACGGTG+AGG - chr8_3:3259632-3259651 None:intergenic 50.0%
CACCGTCCAAGTTCAATCAG+TGG + chr8_3:3259632-3259651 Msa1269920:CDS 50.0%
AGAGAGAGAGAGCTTACCAG+AGG - chr8_3:3259661-3259680 None:intergenic 50.0%
! TCAATCAGTGATGGAGAGGG+TGG + chr8_3:3259751-3259770 Msa1269920:intron 50.0%
GAGCGATGTAGCAACGGTAA+GGG - chr8_3:3259815-3259834 None:intergenic 50.0%
AGAGCGATGTAGCAACGGTA+AGG - chr8_3:3259816-3259835 None:intergenic 50.0%
AGCTTGAAGAGACGTAGGAG+CGG - chr8_3:3259877-3259896 None:intergenic 50.0%
ACAGGATTACACTGGTGCAG+AGG + chr8_3:3260903-3260922 Msa1269920:intron 50.0%
TACACTGATCATGGAGGTCC+AGG + chr8_3:3261033-3261052 Msa1269920:CDS 50.0%
ACACTGATCATGGAGGTCCA+GGG + chr8_3:3261034-3261053 Msa1269920:CDS 50.0%
GTCTCTTCAGGTATGCCTGT+TGG + chr8_3:3261589-3261608 Msa1269920:intron 50.0%
! GCTTTGACGGTGAGCTTTGG+AGG - chr8_3:3259564-3259583 None:intergenic 55.0%
! GCTTGGACGGTGAGCTTTGA+CGG - chr8_3:3259590-3259609 None:intergenic 55.0%
! GTGATGGAGAGGGTGGAATC+TGG + chr8_3:3259758-3259777 Msa1269920:intron 55.0%
TCAGCTCTTACAGGTGGGAG+TGG + chr8_3:3260967-3260986 Msa1269920:intron 55.0%
CAGCTCTTACAGGTGGGAGT+GGG + chr8_3:3260968-3260987 Msa1269920:intron 55.0%
AGGTGGGAGTGGGAAAGTTG+TGG + chr8_3:3260978-3260997 Msa1269920:intron 55.0%
CACTGATCATGGAGGTCCAG+GGG + chr8_3:3261035-3261054 Msa1269920:CDS 55.0%
CATGGAGGTCCAGGGGTTCT+TGG + chr8_3:3261042-3261061 Msa1269920:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 3259455 3262140 3259455 ID=Msa1269920;Name=Msa1269920
chr8_3 mRNA 3259455 3262140 3259455 ID=Msa1269920-mRNA-1;Parent=Msa1269920;Name=Msa1269920-mRNA-1;_AED=0.43;_eAED=0.50;_QI=0|0.16|0|1|0.33|0.42|7|0|276
chr8_3 exon 3259455 3259653 3259455 ID=Msa1269920-mRNA-1:exon:1023;Parent=Msa1269920-mRNA-1
chr8_3 exon 3259767 3259975 3259767 ID=Msa1269920-mRNA-1:exon:1024;Parent=Msa1269920-mRNA-1
chr8_3 exon 3260524 3260662 3260524 ID=Msa1269920-mRNA-1:exon:1025;Parent=Msa1269920-mRNA-1
chr8_3 exon 3260980 3261063 3260980 ID=Msa1269920-mRNA-1:exon:1026;Parent=Msa1269920-mRNA-1
chr8_3 exon 3261599 3261684 3261599 ID=Msa1269920-mRNA-1:exon:1027;Parent=Msa1269920-mRNA-1
chr8_3 exon 3261886 3261916 3261886 ID=Msa1269920-mRNA-1:exon:1028;Parent=Msa1269920-mRNA-1
chr8_3 exon 3262058 3262140 3262058 ID=Msa1269920-mRNA-1:exon:1029;Parent=Msa1269920-mRNA-1
chr8_3 CDS 3259455 3259653 3259455 ID=Msa1269920-mRNA-1:cds;Parent=Msa1269920-mRNA-1
chr8_3 CDS 3259767 3259975 3259767 ID=Msa1269920-mRNA-1:cds;Parent=Msa1269920-mRNA-1
chr8_3 CDS 3260524 3260662 3260524 ID=Msa1269920-mRNA-1:cds;Parent=Msa1269920-mRNA-1
chr8_3 CDS 3260980 3261063 3260980 ID=Msa1269920-mRNA-1:cds;Parent=Msa1269920-mRNA-1
chr8_3 CDS 3261599 3261684 3261599 ID=Msa1269920-mRNA-1:cds;Parent=Msa1269920-mRNA-1
chr8_3 CDS 3261886 3261916 3261886 ID=Msa1269920-mRNA-1:cds;Parent=Msa1269920-mRNA-1
chr8_3 CDS 3262058 3262140 3262058 ID=Msa1269920-mRNA-1:cds;Parent=Msa1269920-mRNA-1
Gene Sequence

>Msa1269920

ATGGAAAAACAAGTTTTCACTTTTACCTTATCCCTTTCCCTATCGCAAAACAGATCCACATTTTTCCTTCTTCACTTTTTCACTCAGTCAAAAAACAACCGTGAAACCTCCAAAGCTCACCGTCAAAGCTCACCGTCAAAGCTCACCGTCCAAGCTCCAATCGTGAATCTCCAACCTCACCGTCCAAGTTCAATCAGTGAGGGTGGAATCTGGAAGAGATATAAGGGTGAAGATGTTCGTGAAACCCTTACCGTTGCTACATCGCTCTTGATCAGTTACAGTTCGTCATCTCCGAAATCAACGATTCCGCTCCTACGTCTCTTCAAGCTGTTAAATTACTCGCTCTCTATTTCCCATCACCCGATTCAAAGGTTACAATTTCTCATCTTTCTCAATTTCTCTTCAATTGCTGATGTTTGTCATGCGTATCAATTATTGAGGAATGGTGGCTTGAAAGAAGAAAACATTATTGTTTTCATGTATGATGATATTGCTTCCAATGTAGAGAATCCAAGGCCTGGAGTCATAATTAACAAACCTGATGGTGGTGGGAGTGGGAAAGTTGTGGATAGTGGTCCCAATGATCATATTTTTGTATACTACACTGATCATGGAGGTCCAGGGGTTCTTGGTATGCCTGTTGGTCCTTACTTGTATGCATCTGATCTGAATGAAGTCTTGAAGAAAAAACATGCTTCTGGATCATATAAGAGCTTAGTATTTTATCTGGAGGCATGTGAATCTGGAACAATTGTTATTTTAATGGAACTCTTGAAATATGATCCAAAGAATCTTTCTCCATCTGAAACGGTGGATAGAGATGGAAACTGA
Protein sequence

>Msa1269920

MEKQVFTFTLSLSLSQNRSTFFLLHFFTQSKNNRETSKAHRQSSPSKLTVQAPIVNLQPHRPSSISEGGIWKRYKGEDVRETLTVATSLLISYSSSSPKSTIPLLRLFKLLNYSLSISHHPIQRLQFLIFLNFSSIADVCHAYQLLRNGGLKEENIIVFMYDDIASNVENPRPGVIINKPDGGGSGKVVDSGPNDHIFVYYTDHGGPGVLGMPVGPYLYASDLNEVLKKKHASGSYKSLVFYLEACESGTIVILMELLKYDPKNLSPSETVDRDGN*