AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1276390


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1276390 MtrunA17_Chr8g0343921 96.135 207 8 0 1 207 1 207 5.79e-145 402
Msa1276390 MtrunA17_Chr8g0343911 59.330 209 82 2 1 207 1 208 1.45e-82 244
Msa1276390 MtrunA17_Chr8g0343934 56.938 209 86 3 1 207 3 209 6.15e-78 232
Msa1276390 MtrunA17_Chr8g0343961 57.282 206 84 3 4 207 6 209 2.07e-77 231
Msa1276390 MtrunA17_Chr8g0343954 58.031 193 77 3 17 207 19 209 1.24e-73 221
Msa1276390 MtrunA17_Chr2g0300561 52.153 209 96 2 1 207 4 210 3.23e-73 220
Msa1276390 MtrunA17_Chr2g0300581 50.718 209 99 2 1 207 4 210 5.70e-73 219
Msa1276390 MtrunA17_Chr2g0300591 50.718 209 99 2 1 207 4 210 2.11e-72 218
Msa1276390 MtrunA17_Chr2g0301611 54.545 209 91 3 1 207 3 209 2.90e-72 218
Msa1276390 MtrunA17_Chr2g0302171 54.854 206 89 3 4 207 6 209 9.76e-71 214
Msa1276390 MtrunA17_Chr3g0084531 54.067 209 92 3 1 207 3 209 5.48e-69 209
Msa1276390 MtrunA17_Chr2g0297231 52.913 206 93 3 1 204 3 206 8.49e-69 209
Msa1276390 MtrunA17_Chr2g0297241 52.885 208 94 3 1 206 3 208 2.07e-68 208
Msa1276390 MtrunA17_Chr2g0313961 53.171 205 92 3 4 206 6 208 2.88e-68 207
Msa1276390 MtrunA17_Chr8g0344051 50.725 207 98 3 4 207 7 212 4.38e-66 202
Msa1276390 MtrunA17_Chr4g0003361 52.913 206 90 5 4 207 5 205 1.63e-65 201
Msa1276390 MtrunA17_Chr3g0084511 52.913 206 92 4 4 207 6 208 2.41e-65 200
Msa1276390 MtrunA17_Chr3g0084521 52.427 206 93 4 4 207 6 208 2.71e-64 197
Msa1276390 MtrunA17_Chr5g0411961 50.239 209 100 3 1 207 3 209 3.84e-64 197
Msa1276390 MtrunA17_Chr2g0302541 53.333 195 87 3 15 207 17 209 7.19e-62 191
Msa1276390 MtrunA17_Chr4g0012651 53.093 194 87 3 16 207 18 209 7.35e-62 191
Msa1276390 MtrunA17_Chr7g0217811 52.577 194 88 3 16 207 18 209 2.43e-60 187
Msa1276390 MtrunA17_Chr8g0344041 49.738 191 93 2 19 207 24 213 2.19e-58 182
Msa1276390 MtrunA17_Chr3g0115141 50.267 187 89 4 22 207 27 210 3.92e-58 182
Msa1276390 MtrunA17_Chr8g0344031 49.038 208 102 3 2 207 6 211 6.04e-58 181
Msa1276390 MtrunA17_Chr2g0286171 47.619 210 103 5 2 207 6 212 1.17e-57 181
Msa1276390 MtrunA17_Chr8g0344021 45.411 207 109 3 4 207 7 212 6.61e-57 179
Msa1276390 MtrunA17_Chr3g0084551 46.798 203 98 4 1 201 3 197 1.88e-53 171
Msa1276390 MtrunA17_Chr1g0178711 36.458 192 108 5 20 200 30 218 2.64e-35 124
Msa1276390 MtrunA17_Chr1g0193421 37.297 185 110 4 22 201 30 213 1.48e-32 117
Msa1276390 MtrunA17_Chr1g0210761 34.211 190 116 4 20 206 27 210 2.14e-30 111
Msa1276390 MtrunA17_Chr2g0292901 35.638 188 112 4 22 203 29 213 1.16e-29 109
Msa1276390 MtrunA17_Chr1g0156131 35.468 203 118 5 8 203 13 209 3.30e-29 108
Msa1276390 MtrunA17_Chr2g0292831 34.555 191 116 4 19 203 25 212 3.43e-29 108
Msa1276390 MtrunA17_Chr7g0271211 37.342 158 97 1 22 177 26 183 9.83e-29 107
Msa1276390 MtrunA17_Chr5g0414971 32.804 189 122 3 22 205 33 221 1.51e-28 106
Msa1276390 MtrunA17_Chr6g0449761 38.421 190 107 6 22 203 28 215 6.23e-28 105
Msa1276390 MtrunA17_Chr6g0449751 38.421 190 107 6 22 203 28 215 6.50e-28 105
Msa1276390 MtrunA17_Chr6g0449731 38.421 190 107 6 22 203 28 215 7.38e-28 104
Msa1276390 MtrunA17_Chr3g0084501 50.980 102 47 2 4 103 6 106 2.31e-27 100
Msa1276390 MtrunA17_Chr1g0189841 36.757 185 106 5 3 177 6 189 2.39e-27 103
Msa1276390 MtrunA17_Chr6g0449771 37.895 190 108 6 22 203 28 215 4.45e-27 102
Msa1276390 MtrunA17_Chr2g0292861 35.465 172 104 4 39 203 7 178 3.03e-26 99.4
Msa1276390 MtrunA17_Chr1g0193671 31.694 183 121 2 22 200 29 211 7.49e-26 99.8
Msa1276390 MtrunA17_Chr2g0292841 35.465 172 104 4 39 203 7 178 2.21e-25 97.4
Msa1276390 MtrunA17_Chr8g0335851 32.308 195 126 4 2 191 11 204 4.94e-25 97.4
Msa1276390 MtrunA17_Chr2g0285871 33.333 210 127 7 1 200 7 213 5.73e-25 97.4
Msa1276390 MtrunA17_Chr4g0007041 34.759 187 111 6 1 179 7 190 9.93e-25 96.7
Msa1276390 MtrunA17_Chr4g0007051 32.857 210 128 7 1 200 7 213 1.24e-24 96.7
Msa1276390 MtrunA17_Chr4g0007031 34.759 187 111 6 1 179 7 190 1.55e-24 96.3
Msa1276390 MtrunA17_Chr5g0419971 34.759 187 111 6 1 179 7 190 1.55e-24 96.3
Msa1276390 MtrunA17_Chr5g0419981 34.759 187 111 6 1 179 7 190 1.55e-24 96.3
Msa1276390 MtrunA17_Chr6g0449791 35.429 175 103 6 1 167 6 178 2.86e-22 90.1
Msa1276390 MtrunA17_Chr3g0130241 35.294 153 80 3 29 167 96 243 5.88e-22 90.9
Msa1276390 MtrunA17_Chr2g0292911 37.607 117 69 2 39 151 7 123 1.65e-20 83.6
Msa1276390 MtrunA17_Chr6g0449781 32.275 189 119 6 22 203 28 214 3.22e-19 82.0
Msa1276390 MtrunA17_Chr2g0321581 30.114 176 104 4 45 203 31 204 8.24e-19 80.9
Msa1276390 MtrunA17_Chr2g0321591 29.545 176 105 4 45 203 31 204 2.51e-18 79.3
Msa1276390 MtrunA17_Chr2g0321601 33.588 131 86 1 74 203 73 203 6.30e-18 78.6
Msa1276390 MtrunA17_Chr4g0026201 31.953 169 112 2 38 203 37 205 4.24e-17 76.3
Msa1276390 MtrunA17_Chr4g0026191 31.953 169 112 2 38 203 37 205 5.85e-17 75.9
Msa1276390 MtrunA17_Chr2g0327651 32.323 99 65 2 41 138 7 104 6.25e-13 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1276390 AT1G72610.1 53.886 193 87 1 17 207 16 208 2.92e-73 221
Msa1276390 AT5G20630.1 50.962 208 99 2 1 206 4 210 7.89e-70 212
Msa1276390 AT1G18980.1 37.073 205 125 2 3 203 14 218 1.99e-41 140
Msa1276390 AT1G18970.1 35.610 205 128 2 1 203 16 218 3.34e-39 134
Msa1276390 AT3G04200.1 39.791 191 107 4 22 204 33 223 1.17e-36 128
Msa1276390 AT3G05930.1 36.813 182 111 3 2 179 8 189 6.27e-33 118
Msa1276390 AT5G26700.1 36.946 203 118 5 4 200 8 206 6.82e-33 118
Msa1276390 AT1G09560.1 34.343 198 126 2 10 203 18 215 3.06e-31 114
Msa1276390 AT3G10080.1 34.715 193 114 5 20 203 30 219 6.08e-31 113
Msa1276390 AT3G10080.2 34.715 193 114 5 20 203 101 290 2.64e-30 113
Msa1276390 AT5G38960.1 36.788 193 111 7 22 204 32 223 2.95e-30 111
Msa1276390 AT1G02335.1 34.810 158 101 1 22 177 30 187 1.26e-29 109
Msa1276390 AT3G04150.1 33.178 214 135 4 2 207 12 225 2.37e-29 109
Msa1276390 AT5G61750.1 33.523 176 101 3 2 167 5 174 3.31e-29 108
Msa1276390 AT1G74820.1 32.558 172 110 1 1 166 15 186 8.04e-29 107
Msa1276390 AT3G62020.1 30.435 207 139 3 3 204 10 216 1.56e-28 107
Msa1276390 AT3G04150.2 31.278 227 135 4 2 207 12 238 2.56e-28 107
Msa1276390 AT3G05950.1 36.842 190 112 4 22 203 32 221 5.47e-28 105
Msa1276390 AT5G39110.1 34.579 214 125 5 5 203 7 220 9.30e-28 105
Msa1276390 AT5G38910.1 33.493 209 129 5 4 203 12 219 6.64e-27 102
Msa1276390 AT5G38940.1 34.211 190 117 4 22 203 32 221 3.17e-26 100
Msa1276390 AT3G04190.1 35.602 191 113 6 22 203 32 221 3.59e-26 100
Msa1276390 AT5G39160.3 34.375 192 112 5 22 204 30 216 4.01e-26 100
Msa1276390 AT5G38940.2 34.211 190 117 4 22 203 56 245 5.72e-26 100
Msa1276390 AT5G39160.1 34.375 192 116 5 22 204 30 220 7.79e-26 100
Msa1276390 AT5G39190.1 34.375 192 116 5 22 204 30 220 9.63e-26 99.8
Msa1276390 AT5G38930.1 34.211 190 117 5 22 203 32 221 1.74e-25 99.0
Msa1276390 AT5G39120.1 31.776 214 134 6 1 203 7 219 2.20e-25 99.0
Msa1276390 AT5G39130.1 33.854 192 117 5 22 204 30 220 1.45e-24 96.7
Msa1276390 AT3G62020.2 31.034 174 115 3 35 203 13 186 2.00e-24 95.5
Msa1276390 AT5G39150.1 30.516 213 137 5 1 203 8 219 2.09e-24 96.3
Msa1276390 AT5G39180.1 30.516 213 137 5 1 203 8 219 3.33e-24 95.9
Msa1276390 AT4G14630.1 34.762 210 125 7 5 203 14 222 4.29e-24 95.5
Msa1276390 AT3G04180.1 34.184 196 109 7 22 203 32 221 1.49e-23 94.0
Msa1276390 AT3G04170.1 34.112 214 119 9 5 203 12 218 1.96e-22 91.3
Msa1276390 AT1G10460.1 33.333 162 101 4 22 176 25 186 4.44e-22 90.1
Msa1276390 AT5G39160.2 34.416 154 95 3 56 204 46 198 1.55e-21 88.2
Msa1276390 AT5G39190.2 34.416 154 95 3 56 204 46 198 1.67e-21 88.2

Find 26 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCAATTTCCAGCTGTTAA+TGG 0.204007 8_3:+11017555 Msa1276390:CDS
GTTTACTCCAACTTCAATTC+AGG 0.252771 8_3:+11017483 Msa1276390:CDS
ATTCTTGATCTTGCTTTGTT+TGG 0.254800 8_3:+11017846 Msa1276390:CDS
ACTTTCTAAAGGTCAAATTT+TGG 0.284643 8_3:+11017728 Msa1276390:CDS
TTTCCAGCTGTTAATGGTCT+TGG 0.322031 8_3:+11017561 Msa1276390:CDS
TCTTCTCCACAAACCCCTTC+AGG 0.334680 8_3:+11017423 Msa1276390:CDS
TAGTACAGAAGCATTGGATA+TGG 0.406392 8_3:-11017379 None:intergenic
GGGTAGCCTGAAGGGGTTTG+TGG 0.459050 8_3:-11017429 None:intergenic
GGAAATTGATTAACAAATGC+AGG 0.482605 8_3:-11017543 None:intergenic
CGCTTCCGAGTTAATGATTG+TGG 0.496243 8_3:+11017647 Msa1276390:CDS
TTTATCAAGACACTTTCTAA+AGG 0.499284 8_3:+11017717 Msa1276390:CDS
GAAGTCTAGTACAGAAGCAT+TGG 0.509708 8_3:-11017385 None:intergenic
AGTGTTTCCTGAATTGAAGT+TGG 0.520987 8_3:-11017490 None:intergenic
GCAAAACTAGACTTAGATGT+TGG 0.536437 8_3:+11017594 Msa1276390:CDS
GTACAAGGTCGTATAACTGC+TGG 0.537303 8_3:+11017669 Msa1276390:CDS
AGATCAAGAATTTGTGAACC+AGG 0.540464 8_3:-11017834 None:intergenic
AAACCAAGACCATTAACAGC+TGG 0.550935 8_3:-11017564 None:intergenic
AAACTAGACTTAGATGTTGG+AGG 0.572259 8_3:+11017597 Msa1276390:CDS
GCGCCGCGATGTGAATGCAT+TGG 0.582043 8_3:-11017627 None:intergenic
TTTGCAAGGGTAGCCTGAAG+GGG 0.597548 8_3:-11017436 None:intergenic
GAGTTAATGATTGTGGTACA+AGG 0.605400 8_3:+11017654 Msa1276390:CDS
GATCAAGAATTTGTGAACCA+GGG 0.620980 8_3:-11017833 None:intergenic
AATGTGAGAGAAGAGTTAAG+AGG 0.621704 8_3:-11017356 None:intergenic
TTGTACCACAATCATTAACT+CGG 0.645177 8_3:-11017652 None:intergenic
TGAAAATGCAACAAACCTTG+TGG 0.665243 8_3:-11017756 None:intergenic
ATACCAATGCATTCACATCG+CGG 0.682883 8_3:+11017624 Msa1276390:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTTACTGATTTTTTGCAA+GGG - chr8_3:11017452-11017471 None:intergenic 25.0%
!!! ATTGTTACTGATTTTTTGCA+AGG - chr8_3:11017453-11017472 None:intergenic 25.0%
! TTTATCAAGACACTTTCTAA+AGG + chr8_3:11017717-11017736 Msa1276390:CDS 25.0%
!!! ACTTTCTAAAGGTCAAATTT+TGG + chr8_3:11017728-11017747 Msa1276390:CDS 25.0%
!! TTGCATTTTCAATATAATGC+TGG + chr8_3:11017768-11017787 Msa1276390:CDS 25.0%
!! ATTTTCAATATAATGCTGGA+AGG + chr8_3:11017772-11017791 Msa1276390:CDS 25.0%
!!! AAAAAGCTTAAGGCTATTTT+TGG + chr8_3:11017927-11017946 Msa1276390:CDS 25.0%
GGAAATTGATTAACAAATGC+AGG - chr8_3:11017546-11017565 None:intergenic 30.0%
AATCAATTTCCAGCTGTTAA+TGG + chr8_3:11017555-11017574 Msa1276390:CDS 30.0%
TTGTACCACAATCATTAACT+CGG - chr8_3:11017655-11017674 None:intergenic 30.0%
! ATTCTTGATCTTGCTTTGTT+TGG + chr8_3:11017846-11017865 Msa1276390:CDS 30.0%
!!! TTAAGCTTTTTAACTTGAGC+AGG - chr8_3:11017918-11017937 None:intergenic 30.0%
TGCTCAAGTTAAAAAGCTTA+AGG + chr8_3:11017917-11017936 Msa1276390:CDS 30.0%
AATGTGAGAGAAGAGTTAAG+AGG - chr8_3:11017359-11017378 None:intergenic 35.0%
!! TAGTACAGAAGCATTGGATA+TGG - chr8_3:11017382-11017401 None:intergenic 35.0%
GTTTACTCCAACTTCAATTC+AGG + chr8_3:11017483-11017502 Msa1276390:CDS 35.0%
AGTGTTTCCTGAATTGAAGT+TGG - chr8_3:11017493-11017512 None:intergenic 35.0%
GCAAAACTAGACTTAGATGT+TGG + chr8_3:11017594-11017613 Msa1276390:CDS 35.0%
AAACTAGACTTAGATGTTGG+AGG + chr8_3:11017597-11017616 Msa1276390:CDS 35.0%
GAGTTAATGATTGTGGTACA+AGG + chr8_3:11017654-11017673 Msa1276390:CDS 35.0%
TGAAAATGCAACAAACCTTG+TGG - chr8_3:11017759-11017778 None:intergenic 35.0%
GATCAAGAATTTGTGAACCA+GGG - chr8_3:11017836-11017855 None:intergenic 35.0%
AGATCAAGAATTTGTGAACC+AGG - chr8_3:11017837-11017856 None:intergenic 35.0%
!!! AAGCTTAAGGCTATTTTTGG+TGG + chr8_3:11017930-11017949 Msa1276390:CDS 35.0%
!!! AGCTTAAGGCTATTTTTGGT+GGG + chr8_3:11017931-11017950 Msa1276390:CDS 35.0%
! GAAGTCTAGTACAGAAGCAT+TGG - chr8_3:11017388-11017407 None:intergenic 40.0%
TTTCCAGCTGTTAATGGTCT+TGG + chr8_3:11017561-11017580 Msa1276390:CDS 40.0%
AAACCAAGACCATTAACAGC+TGG - chr8_3:11017567-11017586 None:intergenic 40.0%
ATACCAATGCATTCACATCG+CGG + chr8_3:11017624-11017643 Msa1276390:CDS 40.0%
!!! CAAATTTTGGCTCTACCACA+AGG + chr8_3:11017741-11017760 Msa1276390:CDS 40.0%
! ACTTTTAGTAGTGAGAACCC+TGG + chr8_3:11017816-11017835 Msa1276390:CDS 40.0%
! TTTTGCAAGGGTAGCCTGAA+GGG - chr8_3:11017440-11017459 None:intergenic 45.0%
! TTTTTGCAAGGGTAGCCTGA+AGG - chr8_3:11017441-11017460 None:intergenic 45.0%
CGCTTCCGAGTTAATGATTG+TGG + chr8_3:11017647-11017666 Msa1276390:CDS 45.0%
GTACAAGGTCGTATAACTGC+TGG + chr8_3:11017669-11017688 Msa1276390:CDS 45.0%
!!! AAGGCTATTTTTGGTGGGAG+TGG + chr8_3:11017936-11017955 Msa1276390:CDS 45.0%
TCTTCTCCACAAACCCCTTC+AGG + chr8_3:11017423-11017442 Msa1276390:CDS 50.0%
TTTGCAAGGGTAGCCTGAAG+GGG - chr8_3:11017439-11017458 None:intergenic 50.0%
GGGTAGCCTGAAGGGGTTTG+TGG - chr8_3:11017432-11017451 None:intergenic 60.0%
GCGCCGCGATGTGAATGCAT+TGG - chr8_3:11017630-11017649 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 11017339 11017962 11017339 ID=Msa1276390;Name=Msa1276390
chr8_3 mRNA 11017339 11017962 11017339 ID=Msa1276390-mRNA-1;Parent=Msa1276390;Name=Msa1276390-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|207
chr8_3 exon 11017339 11017962 11017339 ID=Msa1276390-mRNA-1:exon:4528;Parent=Msa1276390-mRNA-1
chr8_3 CDS 11017339 11017962 11017339 ID=Msa1276390-mRNA-1:cds;Parent=Msa1276390-mRNA-1
Gene Sequence

>Msa1276390

ATGATTTTCATTCTTTTCCTCTTAACTCTTCTCTCACATTCCATATCCAATGCTTCTGTACTAGACTTCTGTGTAGCAGATTTATCTTCTCCACAAACCCCTTCAGGCTACCCTTGCAAAAAATCAGTAACAATCAATGACTTTGTTTACTCCAACTTCAATTCAGGAAACACTTCAGCAAATTTTTTCAAATTTTCAATAACTCCTGCATTTGTTAATCAATTTCCAGCTGTTAATGGTCTTGGTTTATCAGCAGCAAAACTAGACTTAGATGTTGGAGGTATAATACCAATGCATTCACATCGCGGCGCTTCCGAGTTAATGATTGTGGTACAAGGTCGTATAACTGCTGGTTTTATTTCTTCTGATAATACTGTTTTTATCAAGACACTTTCTAAAGGTCAAATTTTGGCTCTACCACAAGGTTTGTTGCATTTTCAATATAATGCTGGAAGGAATAAAGCTTCTGCTTATCTTACTTTTAGTAGTGAGAACCCTGGTTCACAAATTCTTGATCTTGCTTTGTTTGGAAATAATTTGAGTTCTGCTAATGTGATAAAGAGTACTTTGCTTGATCCTGCTCAAGTTAAAAAGCTTAAGGCTATTTTTGGTGGGAGTGGCTAG

Protein sequence

>Msa1276390

MIFILFLLTLLSHSISNASVLDFCVADLSSPQTPSGYPCKKSVTINDFVYSNFNSGNTSANFFKFSITPAFVNQFPAVNGLGLSAAKLDLDVGGIIPMHSHRGASELMIVVQGRITAGFISSDNTVFIKTLSKGQILALPQGLLHFQYNAGRNKASAYLTFSSENPGSQILDLALFGNNLSSANVIKSTLLDPAQVKKLKAIFGGSG*