AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1278020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1278020 MtrunA17_Chr8g0340391 95.370 108 5 0 1 108 1 108 1.09e-71 208
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 28 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATGAGTCTCCTCCTTTAT+TGG 0.158461 8_3:+13969594 None:intergenic
CAATAAAGGGAGAGATTTAT+TGG 0.232920 8_3:+13969370 None:intergenic
TACCTCTTGCCCCATTGAAT+TGG 0.340221 8_3:-13970992 Msa1278020:CDS
AACATAACACAAAATCAAAA+TGG 0.353429 8_3:+13969423 None:intergenic
CATTTGCAAACTTCCTCATA+AGG 0.389007 8_3:+13969510 None:intergenic
GGGGATTGTGCATCCAATAA+AGG 0.414367 8_3:-13969607 Msa1278020:CDS
TTGCTTTATAGATCATATCT+TGG 0.417467 8_3:-13971037 Msa1278020:five_prime_UTR
GTCCTAGGCTCCAATTCAAT+GGG 0.432165 8_3:+13970982 None:intergenic
AGTCCTAGGCTCCAATTCAA+TGG 0.452850 8_3:+13970981 None:intergenic
GAACAAGCGAATGCATTTCA+TGG 0.468472 8_3:+13969300 None:intergenic
ATTTGCAAACTTCCTCATAA+GGG 0.483324 8_3:+13969511 None:intergenic
AATAAAGGAGGAGACTCATA+TGG 0.487460 8_3:-13969592 Msa1278020:CDS
TGTGTTATGTTAGCTGCTCA+TGG 0.490945 8_3:-13969411 Msa1278020:three_prime_UTR
AGCATCTGCTTTGTTTGTTG+AGG 0.495375 8_3:-13970587 Msa1278020:intron
GGAACTAGCTGCAGAGGTGG+TGG 0.502880 8_3:-13970629 Msa1278020:CDS
GACAGAGATAGTCCCTTATG+AGG 0.507264 8_3:-13969523 Msa1278020:CDS
AATCAAAATGGTGCAGAAAG+TGG 0.532595 8_3:+13969435 None:intergenic
AACAAAGCAGATGCTTCACT+TGG 0.572461 8_3:+13970595 None:intergenic
GCCCCATTGAATTGGAGCCT+AGG 0.574612 8_3:-13970984 Msa1278020:CDS
CTCCAATTCAATGGGGCAAG+AGG 0.578963 8_3:+13970990 None:intergenic
AAGTGCAGCTAACCGAAGCA+AGG 0.579958 8_3:-13970951 Msa1278020:CDS
TGCACTTCATTCAAAGTCCT+AGG 0.580697 8_3:+13970967 None:intergenic
AACAAGCGAATGCATTTCAT+GGG 0.584317 8_3:+13969301 None:intergenic
CATCTAGGAACTAGCTGCAG+AGG 0.595710 8_3:-13970635 Msa1278020:intron
CTAGGAACTAGCTGCAGAGG+TGG 0.603510 8_3:-13970632 Msa1278020:intron
AGTGCAGCTAACCGAAGCAA+GGG 0.654525 8_3:-13970950 Msa1278020:intron
GATTGTGCATCCAATAAAGG+AGG 0.673023 8_3:-13969604 Msa1278020:CDS
TCCTAGGCTCCAATTCAATG+GGG 0.743024 8_3:+13970983 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTAATAATTTCTTTTG+AGG + chr8_3:13970212-13970231 None:intergenic 10.0%
!! AAAAAAAATCAAGAAAAAAT+AGG + chr8_3:13971063-13971082 None:intergenic 10.0%
!!! ATATTTAATAATTTCTTTTG+AGG + chr8_3:13970212-13970231 None:intergenic 10.0%
!! AAAAAAAATCAAGAAAAAAT+AGG + chr8_3:13971063-13971082 None:intergenic 10.0%
!!! AATTTTCAAACAAAAGAAAA+TGG - chr8_3:13969644-13969663 Msa1278020:intron 15.0%
!!! ATTTGTTATCAAACTTTTAA+AGG - chr8_3:13969853-13969872 Msa1278020:intron 15.0%
!! ATGTAATGATATAGAATTAT+TGG + chr8_3:13970241-13970260 None:intergenic 15.0%
!!! ATTATAGGAATAATTTTTTG+AGG + chr8_3:13970628-13970647 None:intergenic 15.0%
!! TTATTAGCAAATTGAAAAAT+TGG + chr8_3:13970994-13971013 None:intergenic 15.0%
!!! TCTTGATTTTTTTTTTTGTA+CGG - chr8_3:13971069-13971088 Msa1278020:five_prime_UTR 15.0%
!!! AATTTTCAAACAAAAGAAAA+TGG - chr8_3:13969644-13969663 Msa1278020:intron 15.0%
!!! ATTTGTTATCAAACTTTTAA+AGG - chr8_3:13969853-13969872 Msa1278020:intron 15.0%
!! ATGTAATGATATAGAATTAT+TGG + chr8_3:13970241-13970260 None:intergenic 15.0%
!!! ATTATAGGAATAATTTTTTG+AGG + chr8_3:13970628-13970647 None:intergenic 15.0%
!! TTATTAGCAAATTGAAAAAT+TGG + chr8_3:13970994-13971013 None:intergenic 15.0%
!!! TCTTGATTTTTTTTTTTGTA+CGG - chr8_3:13971069-13971088 Msa1278020:five_prime_UTR 15.0%
!! AATCATCTACTTTATAGTAT+AGG + chr8_3:13969812-13969831 None:intergenic 20.0%
!!! AAAGTTTGATAACAAATGTA+AGG + chr8_3:13969850-13969869 None:intergenic 20.0%
!!! AATAAATTGCTCATTTTCAT+TGG + chr8_3:13969926-13969945 None:intergenic 20.0%
!! AAAATGAGCAATTTATTTGT+GGG - chr8_3:13969929-13969948 Msa1278020:intron 20.0%
!! CAAAACAATATATAAGCATT+TGG + chr8_3:13970664-13970683 None:intergenic 20.0%
!! AAAACACTATTTCATTACTA+AGG + chr8_3:13970696-13970715 None:intergenic 20.0%
!! AACATAACACAAAATCAAAA+TGG + chr8_3:13970918-13970937 None:intergenic 20.0%
!! ATTGAAAAATTGGCAATAAA+GGG + chr8_3:13970984-13971003 None:intergenic 20.0%
!! AATTGAAAAATTGGCAATAA+AGG + chr8_3:13970985-13971004 None:intergenic 20.0%
!!! ACGGATAATTTTTTAGATAA+AGG - chr8_3:13971088-13971107 Msa1278020:five_prime_UTR 20.0%
!! AATCATCTACTTTATAGTAT+AGG + chr8_3:13969812-13969831 None:intergenic 20.0%
!!! AAAGTTTGATAACAAATGTA+AGG + chr8_3:13969850-13969869 None:intergenic 20.0%
!!! AATAAATTGCTCATTTTCAT+TGG + chr8_3:13969926-13969945 None:intergenic 20.0%
!! AAAATGAGCAATTTATTTGT+GGG - chr8_3:13969929-13969948 Msa1278020:intron 20.0%
!! CAAAACAATATATAAGCATT+TGG + chr8_3:13970664-13970683 None:intergenic 20.0%
!! AAAACACTATTTCATTACTA+AGG + chr8_3:13970696-13970715 None:intergenic 20.0%
!! AACATAACACAAAATCAAAA+TGG + chr8_3:13970918-13970937 None:intergenic 20.0%
!! ATTGAAAAATTGGCAATAAA+GGG + chr8_3:13970984-13971003 None:intergenic 20.0%
!! AATTGAAAAATTGGCAATAA+AGG + chr8_3:13970985-13971004 None:intergenic 20.0%
!!! ACGGATAATTTTTTAGATAA+AGG - chr8_3:13971088-13971107 Msa1278020:five_prime_UTR 20.0%
! TTGCTTTATAGATCATATCT+TGG - chr8_3:13969301-13969320 Msa1278020:three_prime_UTR 25.0%
! TGTTTATGATCAAGATTGAA+TGG - chr8_3:13969492-13969511 Msa1278020:CDS 25.0%
!!! TAATGTTTGTTGTTTCATCT+AGG - chr8_3:13969688-13969707 Msa1278020:intron 25.0%
!! AGATTACCTTTGAAAAATAG+TGG - chr8_3:13969884-13969903 Msa1278020:intron 25.0%
!! GATTACCTTTGAAAAATAGT+GGG - chr8_3:13969885-13969904 Msa1278020:intron 25.0%
!!! AATTACCCACTATTTTTCAA+AGG + chr8_3:13969893-13969912 None:intergenic 25.0%
! GAAAATGAGCAATTTATTTG+TGG - chr8_3:13969928-13969947 Msa1278020:intron 25.0%
! AAATGAGCAATTTATTTGTG+GGG - chr8_3:13969930-13969949 Msa1278020:intron 25.0%
!!! CAATGAACATTCACTTTTTT+AGG + chr8_3:13969978-13969997 None:intergenic 25.0%
! CTAAAAAAGTGAATGTTCAT+TGG - chr8_3:13969976-13969995 Msa1278020:intron 25.0%
!! TACTCTAGTTTGAAAAATAG+TGG - chr8_3:13970031-13970050 Msa1278020:intron 25.0%
!! ACTCTAGTTTGAAAAATAGT+GGG - chr8_3:13970032-13970051 Msa1278020:intron 25.0%
! TCATTGTGGAGTAAATATAT+GGG + chr8_3:13970361-13970380 None:intergenic 25.0%
! TATTTAACACCACCTTAAAT+AGG + chr8_3:13970389-13970408 None:intergenic 25.0%
!! TATTTAAGGTGGTGTTAAAT+AGG - chr8_3:13970388-13970407 Msa1278020:intron 25.0%
!!! AATTTTTTGAGGAGTATACA+AGG + chr8_3:13970617-13970636 None:intergenic 25.0%
! AACAATATATAAGCATTTGG+AGG + chr8_3:13970661-13970680 None:intergenic 25.0%
!!! TGTTTTATTGATGTTCATGA+AGG - chr8_3:13970710-13970729 Msa1278020:intron 25.0%
!!! GTTTTATTGATGTTCATGAA+GGG - chr8_3:13970711-13970730 Msa1278020:intron 25.0%
!! TTTTATTGATGTTCATGAAG+GGG - chr8_3:13970712-13970731 Msa1278020:intron 25.0%
! TTGCTTTATAGATCATATCT+TGG - chr8_3:13969301-13969320 Msa1278020:three_prime_UTR 25.0%
! TGTTTATGATCAAGATTGAA+TGG - chr8_3:13969492-13969511 Msa1278020:CDS 25.0%
!!! TAATGTTTGTTGTTTCATCT+AGG - chr8_3:13969688-13969707 Msa1278020:intron 25.0%
!! AGATTACCTTTGAAAAATAG+TGG - chr8_3:13969884-13969903 Msa1278020:intron 25.0%
!! GATTACCTTTGAAAAATAGT+GGG - chr8_3:13969885-13969904 Msa1278020:intron 25.0%
!!! AATTACCCACTATTTTTCAA+AGG + chr8_3:13969893-13969912 None:intergenic 25.0%
! GAAAATGAGCAATTTATTTG+TGG - chr8_3:13969928-13969947 Msa1278020:intron 25.0%
! AAATGAGCAATTTATTTGTG+GGG - chr8_3:13969930-13969949 Msa1278020:intron 25.0%
!!! CAATGAACATTCACTTTTTT+AGG + chr8_3:13969978-13969997 None:intergenic 25.0%
! CTAAAAAAGTGAATGTTCAT+TGG - chr8_3:13969976-13969995 Msa1278020:intron 25.0%
!! TACTCTAGTTTGAAAAATAG+TGG - chr8_3:13970031-13970050 Msa1278020:intron 25.0%
!! ACTCTAGTTTGAAAAATAGT+GGG - chr8_3:13970032-13970051 Msa1278020:intron 25.0%
! TCATTGTGGAGTAAATATAT+GGG + chr8_3:13970361-13970380 None:intergenic 25.0%
! TATTTAACACCACCTTAAAT+AGG + chr8_3:13970389-13970408 None:intergenic 25.0%
!! TATTTAAGGTGGTGTTAAAT+AGG - chr8_3:13970388-13970407 Msa1278020:intron 25.0%
!!! AATTTTTTGAGGAGTATACA+AGG + chr8_3:13970617-13970636 None:intergenic 25.0%
! AACAATATATAAGCATTTGG+AGG + chr8_3:13970661-13970680 None:intergenic 25.0%
!!! TGTTTTATTGATGTTCATGA+AGG - chr8_3:13970710-13970729 Msa1278020:intron 25.0%
!!! GTTTTATTGATGTTCATGAA+GGG - chr8_3:13970711-13970730 Msa1278020:intron 25.0%
!! TTTTATTGATGTTCATGAAG+GGG - chr8_3:13970712-13970731 Msa1278020:intron 25.0%
!! AAAAGACATTGCTTTTGTGA+TGG - chr8_3:13969252-13969271 Msa1278020:three_prime_UTR 30.0%
!! AAAGACATTGCTTTTGTGAT+GGG - chr8_3:13969253-13969272 Msa1278020:three_prime_UTR 30.0%
!!! CTTGGTTTTGTTCTAAACAA+TGG - chr8_3:13969319-13969338 Msa1278020:three_prime_UTR 30.0%
! GATATTTTATACCCTTGCTT+CGG + chr8_3:13969402-13969421 None:intergenic 30.0%
ATTGAATGGTTCAGATTTCA+AGG - chr8_3:13969506-13969525 Msa1278020:CDS 30.0%
GTCGTTTGAATGATATAGAA+TGG + chr8_3:13969583-13969602 None:intergenic 30.0%
!!! TGAACATTCACTTTTTTAGG+TGG + chr8_3:13969975-13969994 None:intergenic 30.0%
CAATTTAAGCATCCAGTTTA+AGG + chr8_3:13970121-13970140 None:intergenic 30.0%
!!! ATAATTTCTTTTGAGGTGCT+GGG + chr8_3:13970205-13970224 None:intergenic 30.0%
!!! AATAATTTCTTTTGAGGTGC+TGG + chr8_3:13970206-13970225 None:intergenic 30.0%
CACAAAAAAATGAAGAGAGA+GGG - chr8_3:13970305-13970324 Msa1278020:intron 30.0%
CTCATTGTGGAGTAAATATA+TGG + chr8_3:13970362-13970381 None:intergenic 30.0%
CATATATTTACTCCACAATG+AGG - chr8_3:13970360-13970379 Msa1278020:intron 30.0%
AATTATAAGACCATCCACAA+TGG + chr8_3:13970425-13970444 None:intergenic 30.0%
! GGAGGAGGATTTTATATTAT+AGG + chr8_3:13970643-13970662 None:intergenic 30.0%
AATATATAAGCATTTGGAGG+AGG + chr8_3:13970658-13970677 None:intergenic 30.0%
ATTTGCAAACTTCCTCATAA+GGG + chr8_3:13970830-13970849 None:intergenic 30.0%
CAATAAAGGGAGAGATTTAT+TGG + chr8_3:13970971-13970990 None:intergenic 30.0%
!! AAAAGACATTGCTTTTGTGA+TGG - chr8_3:13969252-13969271 Msa1278020:three_prime_UTR 30.0%
!! AAAGACATTGCTTTTGTGAT+GGG - chr8_3:13969253-13969272 Msa1278020:three_prime_UTR 30.0%
!!! CTTGGTTTTGTTCTAAACAA+TGG - chr8_3:13969319-13969338 Msa1278020:three_prime_UTR 30.0%
! GATATTTTATACCCTTGCTT+CGG + chr8_3:13969402-13969421 None:intergenic 30.0%
ATTGAATGGTTCAGATTTCA+AGG - chr8_3:13969506-13969525 Msa1278020:CDS 30.0%
GTCGTTTGAATGATATAGAA+TGG + chr8_3:13969583-13969602 None:intergenic 30.0%
!!! TGAACATTCACTTTTTTAGG+TGG + chr8_3:13969975-13969994 None:intergenic 30.0%
CAATTTAAGCATCCAGTTTA+AGG + chr8_3:13970121-13970140 None:intergenic 30.0%
!!! ATAATTTCTTTTGAGGTGCT+GGG + chr8_3:13970205-13970224 None:intergenic 30.0%
!!! AATAATTTCTTTTGAGGTGC+TGG + chr8_3:13970206-13970225 None:intergenic 30.0%
CACAAAAAAATGAAGAGAGA+GGG - chr8_3:13970305-13970324 Msa1278020:intron 30.0%
CTCATTGTGGAGTAAATATA+TGG + chr8_3:13970362-13970381 None:intergenic 30.0%
CATATATTTACTCCACAATG+AGG - chr8_3:13970360-13970379 Msa1278020:intron 30.0%
AATTATAAGACCATCCACAA+TGG + chr8_3:13970425-13970444 None:intergenic 30.0%
! GGAGGAGGATTTTATATTAT+AGG + chr8_3:13970643-13970662 None:intergenic 30.0%
AATATATAAGCATTTGGAGG+AGG + chr8_3:13970658-13970677 None:intergenic 30.0%
ATTTGCAAACTTCCTCATAA+GGG + chr8_3:13970830-13970849 None:intergenic 30.0%
CAATAAAGGGAGAGATTTAT+TGG + chr8_3:13970971-13970990 None:intergenic 30.0%
AATGGTTCAGATTTCAAGGT+CGG - chr8_3:13969510-13969529 Msa1278020:CDS 35.0%
ATAGAATGGTGCAGATATCA+AGG + chr8_3:13969569-13969588 None:intergenic 35.0%
CACGCAATCAATGATGTTTA+TGG + chr8_3:13969605-13969624 None:intergenic 35.0%
!! GCTCATTTTCATTGGTTAGT+GGG + chr8_3:13969918-13969937 None:intergenic 35.0%
!! TGCTCATTTTCATTGGTTAG+TGG + chr8_3:13969919-13969938 None:intergenic 35.0%
! AAGTGAATGTTCATTGGTTG+AGG - chr8_3:13969982-13970001 Msa1278020:intron 35.0%
AATTACCCATCATTCTTCCA+TGG - chr8_3:13970007-13970026 Msa1278020:intron 35.0%
ATTACCCATCATTCTTCCAT+GGG - chr8_3:13970008-13970027 Msa1278020:intron 35.0%
!! TTTTCAAACTAGAGTACCCA+TGG + chr8_3:13970027-13970046 None:intergenic 35.0%
TTATCGTAAAATGAGTGACG+TGG + chr8_3:13970155-13970174 None:intergenic 35.0%
!!! CCTCTCTCTTCATTTTTTTG+TGG + chr8_3:13970307-13970326 None:intergenic 35.0%
CCACAAAAAAATGAAGAGAG+AGG - chr8_3:13970304-13970323 Msa1278020:intron 35.0%
AGTAAATATATGGGTGCCTA+TGG + chr8_3:13970352-13970371 None:intergenic 35.0%
TTAAATAGGCATCCTCATTG+TGG + chr8_3:13970375-13970394 None:intergenic 35.0%
ACAATGAGGATGCCTATTTA+AGG - chr8_3:13970374-13970393 Msa1278020:intron 35.0%
ATATGAGTCTCCTCCTTTAT+TGG + chr8_3:13970747-13970766 None:intergenic 35.0%
AATAAAGGAGGAGACTCATA+TGG - chr8_3:13970746-13970765 Msa1278020:intron 35.0%
CATTTGCAAACTTCCTCATA+AGG + chr8_3:13970831-13970850 None:intergenic 35.0%
AATCAAAATGGTGCAGAAAG+TGG + chr8_3:13970906-13970925 None:intergenic 35.0%
AACAAGCGAATGCATTTCAT+GGG + chr8_3:13971040-13971059 None:intergenic 35.0%
AATGGTTCAGATTTCAAGGT+CGG - chr8_3:13969510-13969529 Msa1278020:CDS 35.0%
ATAGAATGGTGCAGATATCA+AGG + chr8_3:13969569-13969588 None:intergenic 35.0%
CACGCAATCAATGATGTTTA+TGG + chr8_3:13969605-13969624 None:intergenic 35.0%
!! GCTCATTTTCATTGGTTAGT+GGG + chr8_3:13969918-13969937 None:intergenic 35.0%
!! TGCTCATTTTCATTGGTTAG+TGG + chr8_3:13969919-13969938 None:intergenic 35.0%
! AAGTGAATGTTCATTGGTTG+AGG - chr8_3:13969982-13970001 Msa1278020:intron 35.0%
AATTACCCATCATTCTTCCA+TGG - chr8_3:13970007-13970026 Msa1278020:intron 35.0%
ATTACCCATCATTCTTCCAT+GGG - chr8_3:13970008-13970027 Msa1278020:intron 35.0%
!! TTTTCAAACTAGAGTACCCA+TGG + chr8_3:13970027-13970046 None:intergenic 35.0%
TTATCGTAAAATGAGTGACG+TGG + chr8_3:13970155-13970174 None:intergenic 35.0%
!!! CCTCTCTCTTCATTTTTTTG+TGG + chr8_3:13970307-13970326 None:intergenic 35.0%
CCACAAAAAAATGAAGAGAG+AGG - chr8_3:13970304-13970323 Msa1278020:intron 35.0%
AGTAAATATATGGGTGCCTA+TGG + chr8_3:13970352-13970371 None:intergenic 35.0%
TTAAATAGGCATCCTCATTG+TGG + chr8_3:13970375-13970394 None:intergenic 35.0%
ACAATGAGGATGCCTATTTA+AGG - chr8_3:13970374-13970393 Msa1278020:intron 35.0%
ATATGAGTCTCCTCCTTTAT+TGG + chr8_3:13970747-13970766 None:intergenic 35.0%
AATAAAGGAGGAGACTCATA+TGG - chr8_3:13970746-13970765 Msa1278020:intron 35.0%
CATTTGCAAACTTCCTCATA+AGG + chr8_3:13970831-13970850 None:intergenic 35.0%
AATCAAAATGGTGCAGAAAG+TGG + chr8_3:13970906-13970925 None:intergenic 35.0%
AACAAGCGAATGCATTTCAT+GGG + chr8_3:13971040-13971059 None:intergenic 35.0%
TGCACTTCATTCAAAGTCCT+AGG + chr8_3:13969374-13969393 None:intergenic 40.0%
TCACAAAGCTTTCTCGCAAT+TGG - chr8_3:13969427-13969446 Msa1278020:exon 40.0%
TTTCTCACTACGTGAATGCA+TGG + chr8_3:13969472-13969491 None:intergenic 40.0%
! TCATTGATTGCGTGAATGTC+TGG - chr8_3:13969611-13969630 Msa1278020:intron 40.0%
AACAAAGCAGATGCTTCACT+TGG + chr8_3:13969746-13969765 None:intergenic 40.0%
!! AGCATCTGCTTTGTTTGTTG+AGG - chr8_3:13969751-13969770 Msa1278020:intron 40.0%
AGTATAGGTTTCACACCTGT+GGG + chr8_3:13969797-13969816 None:intergenic 40.0%
TAGTATAGGTTTCACACCTG+TGG + chr8_3:13969798-13969817 None:intergenic 40.0%
AGTACCCATGGAAGAATGAT+GGG + chr8_3:13970015-13970034 None:intergenic 40.0%
ATGAGGATGCCTATTTAAGG+TGG - chr8_3:13970377-13970396 Msa1278020:intron 40.0%
ATAAGACCATCCACAATGGT+GGG + chr8_3:13970421-13970440 None:intergenic 40.0%
TATAAGACCATCCACAATGG+TGG + chr8_3:13970422-13970441 None:intergenic 40.0%
ATCTTACAACAAGCCTAGCT+AGG - chr8_3:13970540-13970559 Msa1278020:intron 40.0%
! TCTTTTCTGTTGTCCTAGCT+AGG + chr8_3:13970556-13970575 None:intergenic 40.0%
GATTGTGCATCCAATAAAGG+AGG - chr8_3:13970734-13970753 Msa1278020:intron 40.0%
TGTGTTATGTTAGCTGCTCA+TGG - chr8_3:13970927-13970946 Msa1278020:intron 40.0%
GAACAAGCGAATGCATTTCA+TGG + chr8_3:13971041-13971060 None:intergenic 40.0%
TGCACTTCATTCAAAGTCCT+AGG + chr8_3:13969374-13969393 None:intergenic 40.0%
TCACAAAGCTTTCTCGCAAT+TGG - chr8_3:13969427-13969446 Msa1278020:exon 40.0%
TTTCTCACTACGTGAATGCA+TGG + chr8_3:13969472-13969491 None:intergenic 40.0%
! TCATTGATTGCGTGAATGTC+TGG - chr8_3:13969611-13969630 Msa1278020:intron 40.0%
AACAAAGCAGATGCTTCACT+TGG + chr8_3:13969746-13969765 None:intergenic 40.0%
!! AGCATCTGCTTTGTTTGTTG+AGG - chr8_3:13969751-13969770 Msa1278020:intron 40.0%
AGTATAGGTTTCACACCTGT+GGG + chr8_3:13969797-13969816 None:intergenic 40.0%
TAGTATAGGTTTCACACCTG+TGG + chr8_3:13969798-13969817 None:intergenic 40.0%
AGTACCCATGGAAGAATGAT+GGG + chr8_3:13970015-13970034 None:intergenic 40.0%
ATGAGGATGCCTATTTAAGG+TGG - chr8_3:13970377-13970396 Msa1278020:intron 40.0%
ATAAGACCATCCACAATGGT+GGG + chr8_3:13970421-13970440 None:intergenic 40.0%
TATAAGACCATCCACAATGG+TGG + chr8_3:13970422-13970441 None:intergenic 40.0%
ATCTTACAACAAGCCTAGCT+AGG - chr8_3:13970540-13970559 Msa1278020:intron 40.0%
! TCTTTTCTGTTGTCCTAGCT+AGG + chr8_3:13970556-13970575 None:intergenic 40.0%
GATTGTGCATCCAATAAAGG+AGG - chr8_3:13970734-13970753 Msa1278020:intron 40.0%
TGTGTTATGTTAGCTGCTCA+TGG - chr8_3:13970927-13970946 Msa1278020:intron 40.0%
GAACAAGCGAATGCATTTCA+TGG + chr8_3:13971041-13971060 None:intergenic 40.0%
TACCTCTTGCCCCATTGAAT+TGG - chr8_3:13969346-13969365 Msa1278020:three_prime_UTR 45.0%
TCCTAGGCTCCAATTCAATG+GGG + chr8_3:13969358-13969377 None:intergenic 45.0%
GTCCTAGGCTCCAATTCAAT+GGG + chr8_3:13969359-13969378 None:intergenic 45.0%
AGTCCTAGGCTCCAATTCAA+TGG + chr8_3:13969360-13969379 None:intergenic 45.0%
GAGTACCCATGGAAGAATGA+TGG + chr8_3:13970016-13970035 None:intergenic 45.0%
! TTAAGGTGCTCTGTTGTGGA+TGG + chr8_3:13970104-13970123 None:intergenic 45.0%
! CAGTTTAAGGTGCTCTGTTG+TGG + chr8_3:13970108-13970127 None:intergenic 45.0%
! CACAACAGAGCACCTTAAAC+TGG - chr8_3:13970106-13970125 Msa1278020:intron 45.0%
!!! TTTCTTTTGAGGTGCTGGGT+TGG + chr8_3:13970201-13970220 None:intergenic 45.0%
ATATGGGTGCCTATGGAGAA+GGG + chr8_3:13970345-13970364 None:intergenic 45.0%
TATATGGGTGCCTATGGAGA+AGG + chr8_3:13970346-13970365 None:intergenic 45.0%
GGGGATTGTGCATCCAATAA+AGG - chr8_3:13970731-13970750 Msa1278020:intron 45.0%
GACAGAGATAGTCCCTTATG+AGG - chr8_3:13970815-13970834 Msa1278020:intron 45.0%
TACCTCTTGCCCCATTGAAT+TGG - chr8_3:13969346-13969365 Msa1278020:three_prime_UTR 45.0%
TCCTAGGCTCCAATTCAATG+GGG + chr8_3:13969358-13969377 None:intergenic 45.0%
GTCCTAGGCTCCAATTCAAT+GGG + chr8_3:13969359-13969378 None:intergenic 45.0%
AGTCCTAGGCTCCAATTCAA+TGG + chr8_3:13969360-13969379 None:intergenic 45.0%
GAGTACCCATGGAAGAATGA+TGG + chr8_3:13970016-13970035 None:intergenic 45.0%
! TTAAGGTGCTCTGTTGTGGA+TGG + chr8_3:13970104-13970123 None:intergenic 45.0%
! CAGTTTAAGGTGCTCTGTTG+TGG + chr8_3:13970108-13970127 None:intergenic 45.0%
! CACAACAGAGCACCTTAAAC+TGG - chr8_3:13970106-13970125 Msa1278020:intron 45.0%
!!! TTTCTTTTGAGGTGCTGGGT+TGG + chr8_3:13970201-13970220 None:intergenic 45.0%
ATATGGGTGCCTATGGAGAA+GGG + chr8_3:13970345-13970364 None:intergenic 45.0%
TATATGGGTGCCTATGGAGA+AGG + chr8_3:13970346-13970365 None:intergenic 45.0%
GGGGATTGTGCATCCAATAA+AGG - chr8_3:13970731-13970750 Msa1278020:intron 45.0%
GACAGAGATAGTCCCTTATG+AGG - chr8_3:13970815-13970834 Msa1278020:intron 45.0%
!! TTTATTAAGATTTATTAAAT+AGG - chr8_3:13970280-13970299 Msa1278020:intron 5.0%
!! TTTATTAAGATTTATTAAAT+AGG - chr8_3:13970280-13970299 Msa1278020:intron 5.0%
CTCCAATTCAATGGGGCAAG+AGG + chr8_3:13969351-13969370 None:intergenic 50.0%
AAGTGCAGCTAACCGAAGCA+AGG - chr8_3:13969387-13969406 Msa1278020:three_prime_UTR 50.0%
AGTGCAGCTAACCGAAGCAA+GGG - chr8_3:13969388-13969407 Msa1278020:three_prime_UTR 50.0%
CATCTAGGAACTAGCTGCAG+AGG - chr8_3:13969703-13969722 Msa1278020:intron 50.0%
TTTCCCAATACTCCACCCAC+AGG - chr8_3:13969779-13969798 Msa1278020:intron 50.0%
! ACACCTGTGGGTGGAGTATT+GGG + chr8_3:13969785-13969804 None:intergenic 50.0%
ATAGGTTTCACACCTGTGGG+TGG + chr8_3:13969794-13969813 None:intergenic 50.0%
CCTATGGAGAAGGGTGGTTA+TGG + chr8_3:13970336-13970355 None:intergenic 50.0%
CCATAACCACCCTTCTCCAT+AGG - chr8_3:13970333-13970352 Msa1278020:intron 50.0%
TAAGCACCCACCATTGTGGA+TGG - chr8_3:13970412-13970431 Msa1278020:intron 50.0%
CTCCAATTCAATGGGGCAAG+AGG + chr8_3:13969351-13969370 None:intergenic 50.0%
AAGTGCAGCTAACCGAAGCA+AGG - chr8_3:13969387-13969406 Msa1278020:three_prime_UTR 50.0%
AGTGCAGCTAACCGAAGCAA+GGG - chr8_3:13969388-13969407 Msa1278020:three_prime_UTR 50.0%
CATCTAGGAACTAGCTGCAG+AGG - chr8_3:13969703-13969722 Msa1278020:intron 50.0%
TTTCCCAATACTCCACCCAC+AGG - chr8_3:13969779-13969798 Msa1278020:intron 50.0%
! ACACCTGTGGGTGGAGTATT+GGG + chr8_3:13969785-13969804 None:intergenic 50.0%
ATAGGTTTCACACCTGTGGG+TGG + chr8_3:13969794-13969813 None:intergenic 50.0%
CCTATGGAGAAGGGTGGTTA+TGG + chr8_3:13970336-13970355 None:intergenic 50.0%
CCATAACCACCCTTCTCCAT+AGG - chr8_3:13970333-13970352 Msa1278020:intron 50.0%
TAAGCACCCACCATTGTGGA+TGG - chr8_3:13970412-13970431 Msa1278020:intron 50.0%
GCCCCATTGAATTGGAGCCT+AGG - chr8_3:13969354-13969373 Msa1278020:three_prime_UTR 55.0%
CTAGGAACTAGCTGCAGAGG+TGG - chr8_3:13969706-13969725 Msa1278020:intron 55.0%
CACACCTGTGGGTGGAGTAT+TGG + chr8_3:13969786-13969805 None:intergenic 55.0%
AGGCTAAGCACCCACCATTG+TGG - chr8_3:13970408-13970427 Msa1278020:intron 55.0%
GCCCCATTGAATTGGAGCCT+AGG - chr8_3:13969354-13969373 Msa1278020:three_prime_UTR 55.0%
CTAGGAACTAGCTGCAGAGG+TGG - chr8_3:13969706-13969725 Msa1278020:intron 55.0%
CACACCTGTGGGTGGAGTAT+TGG + chr8_3:13969786-13969805 None:intergenic 55.0%
AGGCTAAGCACCCACCATTG+TGG - chr8_3:13970408-13970427 Msa1278020:intron 55.0%
GGAACTAGCTGCAGAGGTGG+TGG - chr8_3:13969709-13969728 Msa1278020:intron 60.0%
TGGGTGCCTATGGAGAAGGG+TGG + chr8_3:13970342-13970361 None:intergenic 60.0%
GGAACTAGCTGCAGAGGTGG+TGG - chr8_3:13969709-13969728 Msa1278020:intron 60.0%
TGGGTGCCTATGGAGAAGGG+TGG + chr8_3:13970342-13970361 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 13969246 13971114 13969246 ID=Msa1278020;Name=Msa1278020
chr8_3 mRNA 13969246 13971114 13969246 ID=Msa1278020-mRNA-1;Parent=Msa1278020;Name=Msa1278020-mRNA-1;_AED=0.01;_eAED=0.01;_QI=92|1|1|1|1|1|3|191|108
chr8_3 exon 13969246 13969628 13969246 ID=Msa1278020-mRNA-1:exon:5488;Parent=Msa1278020-mRNA-1
chr8_3 exon 13970588 13970650 13970588 ID=Msa1278020-mRNA-1:exon:5487;Parent=Msa1278020-mRNA-1
chr8_3 exon 13970951 13971114 13970951 ID=Msa1278020-mRNA-1:exon:5486;Parent=Msa1278020-mRNA-1
chr8_3 five_prime_UTR 13971023 13971114 13971023 ID=Msa1278020-mRNA-1:five_prime_utr;Parent=Msa1278020-mRNA-1
chr8_3 CDS 13970951 13971022 13970951 ID=Msa1278020-mRNA-1:cds;Parent=Msa1278020-mRNA-1
chr8_3 CDS 13970588 13970650 13970588 ID=Msa1278020-mRNA-1:cds;Parent=Msa1278020-mRNA-1
chr8_3 CDS 13969437 13969628 13969437 ID=Msa1278020-mRNA-1:cds;Parent=Msa1278020-mRNA-1
chr8_3 three_prime_UTR 13969246 13969436 13969246 ID=Msa1278020-mRNA-1:three_prime_utr;Parent=Msa1278020-mRNA-1
Gene Sequence

>Msa1278020

ATGGCAAATACCTCTTGCCCCATTGAATTGGAGCCTAGGACTTTGAATGAAGTGCAGCTAACCGAAGCAAGGGAACTAGCTGCAGAGGTGGTGGAAATGTTTGAACCAAGTGAAGCATCTGCTTTGTTTGTTGAGGGGATTGTGCATCCAATAAAGGAGGAGACTCATATGGATGAAAATGAAAAGCAAGTTGAAAAGTTAATTGATATCAAGAAGAAGACAGAGATAGTCCCTTATGAGGAAGTTTGCAAATGTCAATGCTCATGCTCTGCTGAAAGTCAAGCATATATTGTTGATGTAAAAGAGCCACTTTCTGCACCATTTTGA
Protein sequence

>Msa1278020

MANTSCPIELEPRTLNEVQLTEARELAAEVVEMFEPSEASALFVEGIVHPIKEETHMDENEKQVEKLIDIKKKTEIVPYEEVCKCQCSCSAESQAYIVDVKEPLSAPF*