AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1231460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1231460 MtrunA17_Chr8g0340391 95.370 108 5 0 1 108 1 108 1.09e-71 208
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 28 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATGAGTCTCCTCCTTTAT+TGG 0.158461 8_2:+7597849 None:intergenic
CAATAAAGGGAGAGATTTAT+TGG 0.232920 8_2:+7597625 None:intergenic
TACCTCTTGCCCCATTGAAT+TGG 0.340221 8_2:-7599247 Msa1231460:CDS
AACATAACACAAAATCAAAA+TGG 0.353429 8_2:+7597678 None:intergenic
CATTTGCAAACTTCCTCATA+AGG 0.389007 8_2:+7597765 None:intergenic
GAACAAGCAAATGCATTTCA+TGG 0.407094 8_2:+7597555 None:intergenic
GGGGATTGTGCATCCAATAA+AGG 0.414367 8_2:-7597862 Msa1231460:CDS
TTGCTTTATAGATCATATCT+TGG 0.417467 8_2:-7599292 Msa1231460:five_prime_UTR
GTCCTAGGCTCCAATTCAAT+GGG 0.432165 8_2:+7599237 None:intergenic
AGTCCTAGGCTCCAATTCAA+TGG 0.452850 8_2:+7599236 None:intergenic
ATTTGCAAACTTCCTCATAA+GGG 0.483324 8_2:+7597766 None:intergenic
AATAAAGGAGGAGACTCATA+TGG 0.487460 8_2:-7597847 Msa1231460:CDS
TGTGTTATGTTAGCTGCTCA+TGG 0.490945 8_2:-7597666 Msa1231460:three_prime_UTR
AGCATCTGCTTTGTTTGTTG+AGG 0.495375 8_2:-7598842 Msa1231460:intron
GGAACTAGCTGCAGAGGTGG+TGG 0.502880 8_2:-7598884 Msa1231460:CDS
GACAGAGATAGTCCCTTATG+AGG 0.507264 8_2:-7597778 Msa1231460:CDS
AATCAAAATGGTGCAGAAAG+TGG 0.532595 8_2:+7597690 None:intergenic
AACAAAGCAGATGCTTCACT+TGG 0.572461 8_2:+7598850 None:intergenic
GCCCCATTGAATTGGAGCCT+AGG 0.574612 8_2:-7599239 Msa1231460:CDS
CTCCAATTCAATGGGGCAAG+AGG 0.578963 8_2:+7599245 None:intergenic
AAGTGCAGCTAACCGAAGCA+AGG 0.579958 8_2:-7599206 Msa1231460:CDS
TGCACTTCATTCAAAGTCCT+AGG 0.580697 8_2:+7599222 None:intergenic
AACAAGCAAATGCATTTCAT+GGG 0.584317 8_2:+7597556 None:intergenic
CATCTAGGAACTAGCTGCAG+AGG 0.595710 8_2:-7598890 Msa1231460:intron
CTAGGAACTAGCTGCAGAGG+TGG 0.603510 8_2:-7598887 Msa1231460:intron
AGTGCAGCTAACCGAAGCAA+GGG 0.654525 8_2:-7599205 Msa1231460:intron
GATTGTGCATCCAATAAAGG+AGG 0.673023 8_2:-7597859 Msa1231460:CDS
TCCTAGGCTCCAATTCAATG+GGG 0.743024 8_2:+7599238 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTAATAATTTCTTTTG+AGG + chr8_2:7598467-7598486 None:intergenic 10.0%
!! AAAAAAAATCAAGAAAAAAT+AGG + chr8_2:7599318-7599337 None:intergenic 10.0%
!!! ATATTTAATAATTTCTTTTG+AGG + chr8_2:7598467-7598486 None:intergenic 10.0%
!! AAAAAAAATCAAGAAAAAAT+AGG + chr8_2:7599318-7599337 None:intergenic 10.0%
!!! AATTTTCAAACAAAAGAAAA+TGG - chr8_2:7597899-7597918 Msa1231460:intron 15.0%
!!! ATTTGTTATCAAACTTTTAA+AGG - chr8_2:7598108-7598127 Msa1231460:intron 15.0%
!! ATGTAATGATATAGAATTAT+TGG + chr8_2:7598496-7598515 None:intergenic 15.0%
!!! ATTATAGGAATAATTTTTTG+AGG + chr8_2:7598883-7598902 None:intergenic 15.0%
!! TTATTAGCAAATTGAAAAAT+TGG + chr8_2:7599249-7599268 None:intergenic 15.0%
!!! TCTTGATTTTTTTTTTTGTA+CGG - chr8_2:7599324-7599343 Msa1231460:five_prime_UTR 15.0%
!!! AATTTTCAAACAAAAGAAAA+TGG - chr8_2:7597899-7597918 Msa1231460:intron 15.0%
!!! ATTTGTTATCAAACTTTTAA+AGG - chr8_2:7598108-7598127 Msa1231460:intron 15.0%
!! ATGTAATGATATAGAATTAT+TGG + chr8_2:7598496-7598515 None:intergenic 15.0%
!!! ATTATAGGAATAATTTTTTG+AGG + chr8_2:7598883-7598902 None:intergenic 15.0%
!! TTATTAGCAAATTGAAAAAT+TGG + chr8_2:7599249-7599268 None:intergenic 15.0%
!!! TCTTGATTTTTTTTTTTGTA+CGG - chr8_2:7599324-7599343 Msa1231460:five_prime_UTR 15.0%
!! AATCATCTACTTTATAGTAT+AGG + chr8_2:7598067-7598086 None:intergenic 20.0%
!! AAAATGAGCAATTTATTTGT+GGG - chr8_2:7598184-7598203 Msa1231460:intron 20.0%
!! CAAAACAATATATAAGCATT+TGG + chr8_2:7598919-7598938 None:intergenic 20.0%
!! AAAACACTATTTCATTACTA+AGG + chr8_2:7598951-7598970 None:intergenic 20.0%
!! AACATAACACAAAATCAAAA+TGG + chr8_2:7599173-7599192 None:intergenic 20.0%
!! ATTGAAAAATTGGCAATAAA+GGG + chr8_2:7599239-7599258 None:intergenic 20.0%
!! AATTGAAAAATTGGCAATAA+AGG + chr8_2:7599240-7599259 None:intergenic 20.0%
!!! ACGGATAATTTTTTAGATAA+AGG - chr8_2:7599343-7599362 Msa1231460:five_prime_UTR 20.0%
!! AATCATCTACTTTATAGTAT+AGG + chr8_2:7598067-7598086 None:intergenic 20.0%
!! AAAATGAGCAATTTATTTGT+GGG - chr8_2:7598184-7598203 Msa1231460:intron 20.0%
!! CAAAACAATATATAAGCATT+TGG + chr8_2:7598919-7598938 None:intergenic 20.0%
!! AAAACACTATTTCATTACTA+AGG + chr8_2:7598951-7598970 None:intergenic 20.0%
!! AACATAACACAAAATCAAAA+TGG + chr8_2:7599173-7599192 None:intergenic 20.0%
!! ATTGAAAAATTGGCAATAAA+GGG + chr8_2:7599239-7599258 None:intergenic 20.0%
!! AATTGAAAAATTGGCAATAA+AGG + chr8_2:7599240-7599259 None:intergenic 20.0%
!!! ACGGATAATTTTTTAGATAA+AGG - chr8_2:7599343-7599362 Msa1231460:five_prime_UTR 20.0%
! TTGCTTTATAGATCATATCT+TGG - chr8_2:7597556-7597575 Msa1231460:three_prime_UTR 25.0%
! TGTTTATGATCAAGATTGAA+TGG - chr8_2:7597747-7597766 Msa1231460:CDS 25.0%
!!! TAATGTTTGTTGTTTCATCT+AGG - chr8_2:7597943-7597962 Msa1231460:intron 25.0%
!! AGATTACCTTTGAAAAATAG+TGG - chr8_2:7598139-7598158 Msa1231460:intron 25.0%
!! GATTACCTTTGAAAAATAGT+GGG - chr8_2:7598140-7598159 Msa1231460:intron 25.0%
!!! AATTACCCACTATTTTTCAA+AGG + chr8_2:7598148-7598167 None:intergenic 25.0%
! GAAAATGAGCAATTTATTTG+TGG - chr8_2:7598183-7598202 Msa1231460:intron 25.0%
! AAATGAGCAATTTATTTGTG+GGG - chr8_2:7598185-7598204 Msa1231460:intron 25.0%
!!! CAATGAACATTCACTTTTTT+AGG + chr8_2:7598233-7598252 None:intergenic 25.0%
! CTAAAAAAGTGAATGTTCAT+TGG - chr8_2:7598231-7598250 Msa1231460:intron 25.0%
!! TACTCTAGTTTGAAAAATAG+TGG - chr8_2:7598286-7598305 Msa1231460:intron 25.0%
!! ACTCTAGTTTGAAAAATAGT+GGG - chr8_2:7598287-7598306 Msa1231460:intron 25.0%
! TCATTGTGGAGTAAATATAT+GGG + chr8_2:7598616-7598635 None:intergenic 25.0%
! TATTTAACACCACCTTAAAT+AGG + chr8_2:7598644-7598663 None:intergenic 25.0%
!! TATTTAAGGTGGTGTTAAAT+AGG - chr8_2:7598643-7598662 Msa1231460:intron 25.0%
!!! AATTTTTTGAGGAGTATACA+AGG + chr8_2:7598872-7598891 None:intergenic 25.0%
! AACAATATATAAGCATTTGG+AGG + chr8_2:7598916-7598935 None:intergenic 25.0%
!!! TGTTTTATTGATGTTCATGA+AGG - chr8_2:7598965-7598984 Msa1231460:intron 25.0%
!!! GTTTTATTGATGTTCATGAA+GGG - chr8_2:7598966-7598985 Msa1231460:intron 25.0%
!! TTTTATTGATGTTCATGAAG+GGG - chr8_2:7598967-7598986 Msa1231460:intron 25.0%
! TTGCTTTATAGATCATATCT+TGG - chr8_2:7597556-7597575 Msa1231460:three_prime_UTR 25.0%
! TGTTTATGATCAAGATTGAA+TGG - chr8_2:7597747-7597766 Msa1231460:CDS 25.0%
!!! TAATGTTTGTTGTTTCATCT+AGG - chr8_2:7597943-7597962 Msa1231460:intron 25.0%
!! AGATTACCTTTGAAAAATAG+TGG - chr8_2:7598139-7598158 Msa1231460:intron 25.0%
!! GATTACCTTTGAAAAATAGT+GGG - chr8_2:7598140-7598159 Msa1231460:intron 25.0%
!!! AATTACCCACTATTTTTCAA+AGG + chr8_2:7598148-7598167 None:intergenic 25.0%
! GAAAATGAGCAATTTATTTG+TGG - chr8_2:7598183-7598202 Msa1231460:intron 25.0%
! AAATGAGCAATTTATTTGTG+GGG - chr8_2:7598185-7598204 Msa1231460:intron 25.0%
!!! CAATGAACATTCACTTTTTT+AGG + chr8_2:7598233-7598252 None:intergenic 25.0%
! CTAAAAAAGTGAATGTTCAT+TGG - chr8_2:7598231-7598250 Msa1231460:intron 25.0%
!! TACTCTAGTTTGAAAAATAG+TGG - chr8_2:7598286-7598305 Msa1231460:intron 25.0%
!! ACTCTAGTTTGAAAAATAGT+GGG - chr8_2:7598287-7598306 Msa1231460:intron 25.0%
! TCATTGTGGAGTAAATATAT+GGG + chr8_2:7598616-7598635 None:intergenic 25.0%
! TATTTAACACCACCTTAAAT+AGG + chr8_2:7598644-7598663 None:intergenic 25.0%
!! TATTTAAGGTGGTGTTAAAT+AGG - chr8_2:7598643-7598662 Msa1231460:intron 25.0%
!!! AATTTTTTGAGGAGTATACA+AGG + chr8_2:7598872-7598891 None:intergenic 25.0%
! AACAATATATAAGCATTTGG+AGG + chr8_2:7598916-7598935 None:intergenic 25.0%
!!! TGTTTTATTGATGTTCATGA+AGG - chr8_2:7598965-7598984 Msa1231460:intron 25.0%
!!! GTTTTATTGATGTTCATGAA+GGG - chr8_2:7598966-7598985 Msa1231460:intron 25.0%
!! TTTTATTGATGTTCATGAAG+GGG - chr8_2:7598967-7598986 Msa1231460:intron 25.0%
!! AAAAGACATTGCTTTTGTGA+TGG - chr8_2:7597507-7597526 Msa1231460:three_prime_UTR 30.0%
!! AAAGACATTGCTTTTGTGAT+GGG - chr8_2:7597508-7597527 Msa1231460:three_prime_UTR 30.0%
!!! CTTGGTTTTGTTCTAAACAA+TGG - chr8_2:7597574-7597593 Msa1231460:three_prime_UTR 30.0%
! GATATTTTATACCCTTGCTT+CGG + chr8_2:7597657-7597676 None:intergenic 30.0%
ATTGAATGGTTCAGATTTCA+AGG - chr8_2:7597761-7597780 Msa1231460:CDS 30.0%
GTCGTTTGAATGATATAGAA+TGG + chr8_2:7597838-7597857 None:intergenic 30.0%
! GCTCATTTTCATTAGTTAGT+GGG + chr8_2:7598173-7598192 None:intergenic 30.0%
! TGCTCATTTTCATTAGTTAG+TGG + chr8_2:7598174-7598193 None:intergenic 30.0%
!!! TGAACATTCACTTTTTTAGG+TGG + chr8_2:7598230-7598249 None:intergenic 30.0%
CAATTTAAGCATCCAGTTTA+AGG + chr8_2:7598376-7598395 None:intergenic 30.0%
!!! ATAATTTCTTTTGAGGTGCT+GGG + chr8_2:7598460-7598479 None:intergenic 30.0%
!!! AATAATTTCTTTTGAGGTGC+TGG + chr8_2:7598461-7598480 None:intergenic 30.0%
CACAAAAAAATGAAGAGAGA+GGG - chr8_2:7598560-7598579 Msa1231460:intron 30.0%
CTCATTGTGGAGTAAATATA+TGG + chr8_2:7598617-7598636 None:intergenic 30.0%
CATATATTTACTCCACAATG+AGG - chr8_2:7598615-7598634 Msa1231460:intron 30.0%
AATTATAAGACCATCCACAA+TGG + chr8_2:7598680-7598699 None:intergenic 30.0%
! GGAGGAGGATTTTATATTAT+AGG + chr8_2:7598898-7598917 None:intergenic 30.0%
AATATATAAGCATTTGGAGG+AGG + chr8_2:7598913-7598932 None:intergenic 30.0%
ATTTGCAAACTTCCTCATAA+GGG + chr8_2:7599085-7599104 None:intergenic 30.0%
CAATAAAGGGAGAGATTTAT+TGG + chr8_2:7599226-7599245 None:intergenic 30.0%
AACAAGCAAATGCATTTCAT+GGG + chr8_2:7599295-7599314 None:intergenic 30.0%
!! AAAAGACATTGCTTTTGTGA+TGG - chr8_2:7597507-7597526 Msa1231460:three_prime_UTR 30.0%
!! AAAGACATTGCTTTTGTGAT+GGG - chr8_2:7597508-7597527 Msa1231460:three_prime_UTR 30.0%
!!! CTTGGTTTTGTTCTAAACAA+TGG - chr8_2:7597574-7597593 Msa1231460:three_prime_UTR 30.0%
! GATATTTTATACCCTTGCTT+CGG + chr8_2:7597657-7597676 None:intergenic 30.0%
ATTGAATGGTTCAGATTTCA+AGG - chr8_2:7597761-7597780 Msa1231460:CDS 30.0%
GTCGTTTGAATGATATAGAA+TGG + chr8_2:7597838-7597857 None:intergenic 30.0%
! GCTCATTTTCATTAGTTAGT+GGG + chr8_2:7598173-7598192 None:intergenic 30.0%
! TGCTCATTTTCATTAGTTAG+TGG + chr8_2:7598174-7598193 None:intergenic 30.0%
!!! TGAACATTCACTTTTTTAGG+TGG + chr8_2:7598230-7598249 None:intergenic 30.0%
CAATTTAAGCATCCAGTTTA+AGG + chr8_2:7598376-7598395 None:intergenic 30.0%
!!! ATAATTTCTTTTGAGGTGCT+GGG + chr8_2:7598460-7598479 None:intergenic 30.0%
!!! AATAATTTCTTTTGAGGTGC+TGG + chr8_2:7598461-7598480 None:intergenic 30.0%
CACAAAAAAATGAAGAGAGA+GGG - chr8_2:7598560-7598579 Msa1231460:intron 30.0%
CTCATTGTGGAGTAAATATA+TGG + chr8_2:7598617-7598636 None:intergenic 30.0%
CATATATTTACTCCACAATG+AGG - chr8_2:7598615-7598634 Msa1231460:intron 30.0%
AATTATAAGACCATCCACAA+TGG + chr8_2:7598680-7598699 None:intergenic 30.0%
! GGAGGAGGATTTTATATTAT+AGG + chr8_2:7598898-7598917 None:intergenic 30.0%
AATATATAAGCATTTGGAGG+AGG + chr8_2:7598913-7598932 None:intergenic 30.0%
ATTTGCAAACTTCCTCATAA+GGG + chr8_2:7599085-7599104 None:intergenic 30.0%
CAATAAAGGGAGAGATTTAT+TGG + chr8_2:7599226-7599245 None:intergenic 30.0%
AACAAGCAAATGCATTTCAT+GGG + chr8_2:7599295-7599314 None:intergenic 30.0%
AATGGTTCAGATTTCAAGGT+CGG - chr8_2:7597765-7597784 Msa1231460:CDS 35.0%
ATAGAATGGTGCAGATATCA+AGG + chr8_2:7597824-7597843 None:intergenic 35.0%
CACGCAATCAATGATGTTTA+TGG + chr8_2:7597860-7597879 None:intergenic 35.0%
! AAGTGAATGTTCATTGGTTG+AGG - chr8_2:7598237-7598256 Msa1231460:intron 35.0%
AATTACCCATCATTCTTCCA+TGG - chr8_2:7598262-7598281 Msa1231460:intron 35.0%
ATTACCCATCATTCTTCCAT+GGG - chr8_2:7598263-7598282 Msa1231460:intron 35.0%
!! TTTTCAAACTAGAGTACCCA+TGG + chr8_2:7598282-7598301 None:intergenic 35.0%
TTATCGTAAAATGAGTGACG+TGG + chr8_2:7598410-7598429 None:intergenic 35.0%
!!! CCTCTCTCTTCATTTTTTTG+TGG + chr8_2:7598562-7598581 None:intergenic 35.0%
CCACAAAAAAATGAAGAGAG+AGG - chr8_2:7598559-7598578 Msa1231460:intron 35.0%
AGTAAATATATGGGTGCCTA+TGG + chr8_2:7598607-7598626 None:intergenic 35.0%
TTAAATAGGCATCCTCATTG+TGG + chr8_2:7598630-7598649 None:intergenic 35.0%
ACAATGAGGATGCCTATTTA+AGG - chr8_2:7598629-7598648 Msa1231460:intron 35.0%
ATATGAGTCTCCTCCTTTAT+TGG + chr8_2:7599002-7599021 None:intergenic 35.0%
AATAAAGGAGGAGACTCATA+TGG - chr8_2:7599001-7599020 Msa1231460:intron 35.0%
CATTTGCAAACTTCCTCATA+AGG + chr8_2:7599086-7599105 None:intergenic 35.0%
AATCAAAATGGTGCAGAAAG+TGG + chr8_2:7599161-7599180 None:intergenic 35.0%
GAACAAGCAAATGCATTTCA+TGG + chr8_2:7599296-7599315 None:intergenic 35.0%
AATGGTTCAGATTTCAAGGT+CGG - chr8_2:7597765-7597784 Msa1231460:CDS 35.0%
ATAGAATGGTGCAGATATCA+AGG + chr8_2:7597824-7597843 None:intergenic 35.0%
CACGCAATCAATGATGTTTA+TGG + chr8_2:7597860-7597879 None:intergenic 35.0%
! AAGTGAATGTTCATTGGTTG+AGG - chr8_2:7598237-7598256 Msa1231460:intron 35.0%
AATTACCCATCATTCTTCCA+TGG - chr8_2:7598262-7598281 Msa1231460:intron 35.0%
ATTACCCATCATTCTTCCAT+GGG - chr8_2:7598263-7598282 Msa1231460:intron 35.0%
!! TTTTCAAACTAGAGTACCCA+TGG + chr8_2:7598282-7598301 None:intergenic 35.0%
TTATCGTAAAATGAGTGACG+TGG + chr8_2:7598410-7598429 None:intergenic 35.0%
!!! CCTCTCTCTTCATTTTTTTG+TGG + chr8_2:7598562-7598581 None:intergenic 35.0%
CCACAAAAAAATGAAGAGAG+AGG - chr8_2:7598559-7598578 Msa1231460:intron 35.0%
AGTAAATATATGGGTGCCTA+TGG + chr8_2:7598607-7598626 None:intergenic 35.0%
TTAAATAGGCATCCTCATTG+TGG + chr8_2:7598630-7598649 None:intergenic 35.0%
ACAATGAGGATGCCTATTTA+AGG - chr8_2:7598629-7598648 Msa1231460:intron 35.0%
ATATGAGTCTCCTCCTTTAT+TGG + chr8_2:7599002-7599021 None:intergenic 35.0%
AATAAAGGAGGAGACTCATA+TGG - chr8_2:7599001-7599020 Msa1231460:intron 35.0%
CATTTGCAAACTTCCTCATA+AGG + chr8_2:7599086-7599105 None:intergenic 35.0%
AATCAAAATGGTGCAGAAAG+TGG + chr8_2:7599161-7599180 None:intergenic 35.0%
GAACAAGCAAATGCATTTCA+TGG + chr8_2:7599296-7599315 None:intergenic 35.0%
TGCACTTCATTCAAAGTCCT+AGG + chr8_2:7597629-7597648 None:intergenic 40.0%
TCACAAAGCTTTCTCGCAAT+TGG - chr8_2:7597682-7597701 Msa1231460:exon 40.0%
TTTCTCACTACGTGAATGCA+TGG + chr8_2:7597727-7597746 None:intergenic 40.0%
! TCATTGATTGCGTGAATGTC+TGG - chr8_2:7597866-7597885 Msa1231460:intron 40.0%
AACAAAGCAGATGCTTCACT+TGG + chr8_2:7598001-7598020 None:intergenic 40.0%
!! AGCATCTGCTTTGTTTGTTG+AGG - chr8_2:7598006-7598025 Msa1231460:intron 40.0%
AGTATAGGTTTCACACCAGT+GGG + chr8_2:7598052-7598071 None:intergenic 40.0%
TAGTATAGGTTTCACACCAG+TGG + chr8_2:7598053-7598072 None:intergenic 40.0%
AGTACCCATGGAAGAATGAT+GGG + chr8_2:7598270-7598289 None:intergenic 40.0%
ATGAGGATGCCTATTTAAGG+TGG - chr8_2:7598632-7598651 Msa1231460:intron 40.0%
ATAAGACCATCCACAATGGT+GGG + chr8_2:7598676-7598695 None:intergenic 40.0%
TATAAGACCATCCACAATGG+TGG + chr8_2:7598677-7598696 None:intergenic 40.0%
ATCTTACAACAAGCCTAGCT+AGG - chr8_2:7598795-7598814 Msa1231460:intron 40.0%
! TCTTTTCTGTTGTCCTAGCT+AGG + chr8_2:7598811-7598830 None:intergenic 40.0%
GATTGTGCATCCAATAAAGG+AGG - chr8_2:7598989-7599008 Msa1231460:intron 40.0%
TGTGTTATGTTAGCTGCTCA+TGG - chr8_2:7599182-7599201 Msa1231460:intron 40.0%
TGCACTTCATTCAAAGTCCT+AGG + chr8_2:7597629-7597648 None:intergenic 40.0%
TCACAAAGCTTTCTCGCAAT+TGG - chr8_2:7597682-7597701 Msa1231460:exon 40.0%
TTTCTCACTACGTGAATGCA+TGG + chr8_2:7597727-7597746 None:intergenic 40.0%
! TCATTGATTGCGTGAATGTC+TGG - chr8_2:7597866-7597885 Msa1231460:intron 40.0%
AACAAAGCAGATGCTTCACT+TGG + chr8_2:7598001-7598020 None:intergenic 40.0%
!! AGCATCTGCTTTGTTTGTTG+AGG - chr8_2:7598006-7598025 Msa1231460:intron 40.0%
AGTATAGGTTTCACACCAGT+GGG + chr8_2:7598052-7598071 None:intergenic 40.0%
TAGTATAGGTTTCACACCAG+TGG + chr8_2:7598053-7598072 None:intergenic 40.0%
AGTACCCATGGAAGAATGAT+GGG + chr8_2:7598270-7598289 None:intergenic 40.0%
ATGAGGATGCCTATTTAAGG+TGG - chr8_2:7598632-7598651 Msa1231460:intron 40.0%
ATAAGACCATCCACAATGGT+GGG + chr8_2:7598676-7598695 None:intergenic 40.0%
TATAAGACCATCCACAATGG+TGG + chr8_2:7598677-7598696 None:intergenic 40.0%
ATCTTACAACAAGCCTAGCT+AGG - chr8_2:7598795-7598814 Msa1231460:intron 40.0%
! TCTTTTCTGTTGTCCTAGCT+AGG + chr8_2:7598811-7598830 None:intergenic 40.0%
GATTGTGCATCCAATAAAGG+AGG - chr8_2:7598989-7599008 Msa1231460:intron 40.0%
TGTGTTATGTTAGCTGCTCA+TGG - chr8_2:7599182-7599201 Msa1231460:intron 40.0%
TACCTCTTGCCCCATTGAAT+TGG - chr8_2:7597601-7597620 Msa1231460:three_prime_UTR 45.0%
TCCTAGGCTCCAATTCAATG+GGG + chr8_2:7597613-7597632 None:intergenic 45.0%
GTCCTAGGCTCCAATTCAAT+GGG + chr8_2:7597614-7597633 None:intergenic 45.0%
AGTCCTAGGCTCCAATTCAA+TGG + chr8_2:7597615-7597634 None:intergenic 45.0%
GAGTACCCATGGAAGAATGA+TGG + chr8_2:7598271-7598290 None:intergenic 45.0%
! TTAAGGTGCTCTGTTGTGGA+TGG + chr8_2:7598359-7598378 None:intergenic 45.0%
! CAGTTTAAGGTGCTCTGTTG+TGG + chr8_2:7598363-7598382 None:intergenic 45.0%
! CACAACAGAGCACCTTAAAC+TGG - chr8_2:7598361-7598380 Msa1231460:intron 45.0%
!!! TTTCTTTTGAGGTGCTGGGT+TGG + chr8_2:7598456-7598475 None:intergenic 45.0%
ATATGGGTGCCTATGGAGAA+GGG + chr8_2:7598600-7598619 None:intergenic 45.0%
TATATGGGTGCCTATGGAGA+AGG + chr8_2:7598601-7598620 None:intergenic 45.0%
GGGGATTGTGCATCCAATAA+AGG - chr8_2:7598986-7599005 Msa1231460:intron 45.0%
GACAGAGATAGTCCCTTATG+AGG - chr8_2:7599070-7599089 Msa1231460:intron 45.0%
TACCTCTTGCCCCATTGAAT+TGG - chr8_2:7597601-7597620 Msa1231460:three_prime_UTR 45.0%
TCCTAGGCTCCAATTCAATG+GGG + chr8_2:7597613-7597632 None:intergenic 45.0%
GTCCTAGGCTCCAATTCAAT+GGG + chr8_2:7597614-7597633 None:intergenic 45.0%
AGTCCTAGGCTCCAATTCAA+TGG + chr8_2:7597615-7597634 None:intergenic 45.0%
GAGTACCCATGGAAGAATGA+TGG + chr8_2:7598271-7598290 None:intergenic 45.0%
! TTAAGGTGCTCTGTTGTGGA+TGG + chr8_2:7598359-7598378 None:intergenic 45.0%
! CAGTTTAAGGTGCTCTGTTG+TGG + chr8_2:7598363-7598382 None:intergenic 45.0%
! CACAACAGAGCACCTTAAAC+TGG - chr8_2:7598361-7598380 Msa1231460:intron 45.0%
!!! TTTCTTTTGAGGTGCTGGGT+TGG + chr8_2:7598456-7598475 None:intergenic 45.0%
ATATGGGTGCCTATGGAGAA+GGG + chr8_2:7598600-7598619 None:intergenic 45.0%
TATATGGGTGCCTATGGAGA+AGG + chr8_2:7598601-7598620 None:intergenic 45.0%
GGGGATTGTGCATCCAATAA+AGG - chr8_2:7598986-7599005 Msa1231460:intron 45.0%
GACAGAGATAGTCCCTTATG+AGG - chr8_2:7599070-7599089 Msa1231460:intron 45.0%
!! TTTATTAAGATTTATTAAAT+AGG - chr8_2:7598535-7598554 Msa1231460:intron 5.0%
!! TTTATTAAGATTTATTAAAT+AGG - chr8_2:7598535-7598554 Msa1231460:intron 5.0%
CTCCAATTCAATGGGGCAAG+AGG + chr8_2:7597606-7597625 None:intergenic 50.0%
AAGTGCAGCTAACCGAAGCA+AGG - chr8_2:7597642-7597661 Msa1231460:three_prime_UTR 50.0%
AGTGCAGCTAACCGAAGCAA+GGG - chr8_2:7597643-7597662 Msa1231460:three_prime_UTR 50.0%
CATCTAGGAACTAGCTGCAG+AGG - chr8_2:7597958-7597977 Msa1231460:intron 50.0%
TTTCCCAATACTCCACCCAC+TGG - chr8_2:7598034-7598053 Msa1231460:intron 50.0%
! ACACCAGTGGGTGGAGTATT+GGG + chr8_2:7598040-7598059 None:intergenic 50.0%
ATAGGTTTCACACCAGTGGG+TGG + chr8_2:7598049-7598068 None:intergenic 50.0%
CCTATGGAGAAGGGTGGTTA+TGG + chr8_2:7598591-7598610 None:intergenic 50.0%
CCATAACCACCCTTCTCCAT+AGG - chr8_2:7598588-7598607 Msa1231460:intron 50.0%
TAAGCACCCACCATTGTGGA+TGG - chr8_2:7598667-7598686 Msa1231460:intron 50.0%
CTCCAATTCAATGGGGCAAG+AGG + chr8_2:7597606-7597625 None:intergenic 50.0%
AAGTGCAGCTAACCGAAGCA+AGG - chr8_2:7597642-7597661 Msa1231460:three_prime_UTR 50.0%
AGTGCAGCTAACCGAAGCAA+GGG - chr8_2:7597643-7597662 Msa1231460:three_prime_UTR 50.0%
CATCTAGGAACTAGCTGCAG+AGG - chr8_2:7597958-7597977 Msa1231460:intron 50.0%
TTTCCCAATACTCCACCCAC+TGG - chr8_2:7598034-7598053 Msa1231460:intron 50.0%
! ACACCAGTGGGTGGAGTATT+GGG + chr8_2:7598040-7598059 None:intergenic 50.0%
ATAGGTTTCACACCAGTGGG+TGG + chr8_2:7598049-7598068 None:intergenic 50.0%
CCTATGGAGAAGGGTGGTTA+TGG + chr8_2:7598591-7598610 None:intergenic 50.0%
CCATAACCACCCTTCTCCAT+AGG - chr8_2:7598588-7598607 Msa1231460:intron 50.0%
TAAGCACCCACCATTGTGGA+TGG - chr8_2:7598667-7598686 Msa1231460:intron 50.0%
GCCCCATTGAATTGGAGCCT+AGG - chr8_2:7597609-7597628 Msa1231460:three_prime_UTR 55.0%
CTAGGAACTAGCTGCAGAGG+TGG - chr8_2:7597961-7597980 Msa1231460:intron 55.0%
CACACCAGTGGGTGGAGTAT+TGG + chr8_2:7598041-7598060 None:intergenic 55.0%
AGGCTAAGCACCCACCATTG+TGG - chr8_2:7598663-7598682 Msa1231460:intron 55.0%
GCCCCATTGAATTGGAGCCT+AGG - chr8_2:7597609-7597628 Msa1231460:three_prime_UTR 55.0%
CTAGGAACTAGCTGCAGAGG+TGG - chr8_2:7597961-7597980 Msa1231460:intron 55.0%
CACACCAGTGGGTGGAGTAT+TGG + chr8_2:7598041-7598060 None:intergenic 55.0%
AGGCTAAGCACCCACCATTG+TGG - chr8_2:7598663-7598682 Msa1231460:intron 55.0%
GGAACTAGCTGCAGAGGTGG+TGG - chr8_2:7597964-7597983 Msa1231460:intron 60.0%
TGGGTGCCTATGGAGAAGGG+TGG + chr8_2:7598597-7598616 None:intergenic 60.0%
GGAACTAGCTGCAGAGGTGG+TGG - chr8_2:7597964-7597983 Msa1231460:intron 60.0%
TGGGTGCCTATGGAGAAGGG+TGG + chr8_2:7598597-7598616 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 7597501 7599369 7597501 ID=Msa1231460;Name=Msa1231460
chr8_2 mRNA 7597501 7599369 7597501 ID=Msa1231460-mRNA-1;Parent=Msa1231460;Name=Msa1231460-mRNA-1;_AED=0.01;_eAED=0.01;_QI=92|1|1|1|1|1|3|191|108
chr8_2 exon 7597501 7597883 7597501 ID=Msa1231460-mRNA-1:exon:2868;Parent=Msa1231460-mRNA-1
chr8_2 exon 7598843 7598905 7598843 ID=Msa1231460-mRNA-1:exon:2867;Parent=Msa1231460-mRNA-1
chr8_2 exon 7599206 7599369 7599206 ID=Msa1231460-mRNA-1:exon:2866;Parent=Msa1231460-mRNA-1
chr8_2 five_prime_UTR 7599278 7599369 7599278 ID=Msa1231460-mRNA-1:five_prime_utr;Parent=Msa1231460-mRNA-1
chr8_2 CDS 7599206 7599277 7599206 ID=Msa1231460-mRNA-1:cds;Parent=Msa1231460-mRNA-1
chr8_2 CDS 7598843 7598905 7598843 ID=Msa1231460-mRNA-1:cds;Parent=Msa1231460-mRNA-1
chr8_2 CDS 7597692 7597883 7597692 ID=Msa1231460-mRNA-1:cds;Parent=Msa1231460-mRNA-1
chr8_2 three_prime_UTR 7597501 7597691 7597501 ID=Msa1231460-mRNA-1:three_prime_utr;Parent=Msa1231460-mRNA-1
Gene Sequence

>Msa1231460

ATGGCAAATACCTCTTGCCCCATTGAATTGGAGCCTAGGACTTTGAATGAAGTGCAGCTAACCGAAGCAAGGGAACTAGCTGCAGAGGTGGTGGAAATGTTTGAACCAAGTGAAGCATCTGCTTTGTTTGTTGAGGGGATTGTGCATCCAATAAAGGAGGAGACTCATATGGATGAAAATGAAAAGCAAGTTGAAAAGTTAATTGATATCAAGAAGAAGACAGAGATAGTCCCTTATGAGGAAGTTTGCAAATGTCAATGCTCATGCTCTGCTGAAAGTCAAGCATATATTGTTGATGTAAAAGAGCCACTTTCTGCACCATTTTGA
Protein sequence

>Msa1231460

MANTSCPIELEPRTLNEVQLTEARELAAEVVEMFEPSEASALFVEGIVHPIKEETHMDENEKQVEKLIDIKKKTEIVPYEEVCKCQCSCSAESQAYIVDVKEPLSAPF*