AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1278710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1278710 MtrunA17_Chr4g0063511 100.000 98 0 0 1 98 1 98 1.50e-63 203
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1278710 AT5G20170.1 67.708 96 28 1 6 98 4 99 6.62e-34 123

Find 61 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAATTGCTTGAAGTTATT+TGG 0.135968 8_3:-15206849 Msa1278710:three_prime_UTR
TGACTGGGTTGTGGGAATTA+AGG 0.234722 8_3:+15208392 None:intergenic
ACTACCCTCCGCCCCTGTTT+GGG 0.236359 8_3:-15208452 Msa1278710:five_prime_UTR
CCATGCCCTCTTCCATCTTC+TGG 0.246367 8_3:+15207427 None:intergenic
AACATTAAGTTGGGTCTATA+AGG 0.247032 8_3:+15208368 None:intergenic
TTGGATTCCATTGAAGAAAA+TGG 0.254887 8_3:-15207366 Msa1278710:CDS
CACTACCCTCCGCCCCTGTT+TGG 0.256620 8_3:-15208453 Msa1278710:five_prime_UTR
AGTTATTTGGTTTCTGAAAA+AGG 0.262057 8_3:-15206836 Msa1278710:three_prime_UTR
TTTAGGAGTACCTGCTGTTT+TGG 0.270591 8_3:-15208219 Msa1278710:five_prime_UTR
TGAAGATAAGGTGATAGTTC+AGG 0.342552 8_3:+15208482 None:intergenic
TATCACAAAGGTGAGTGATT+TGG 0.361696 8_3:+15208295 None:intergenic
CTTCGATCCCCACCCCAAAC+AGG 0.370873 8_3:+15208439 None:intergenic
AGCCTCAGGTGCAAACAAAA+TGG 0.376865 8_3:+15208144 None:intergenic
CTCCGCCCCTGTTTGGGGTG+GGG 0.410417 8_3:-15208446 Msa1278710:five_prime_UTR
CCTCCGCCCCTGTTTGGGGT+GGG 0.411262 8_3:-15208447 Msa1278710:five_prime_UTR
TCAGGAGAATTGACTTCGCT+TGG 0.414516 8_3:-15207128 Msa1278710:CDS
GAATTTGCAGTTGGCTCACC+AGG 0.421563 8_3:-15207022 Msa1278710:CDS
TAGTTCAGGGACAAAATTGA+TGG 0.427148 8_3:+15208496 None:intergenic
GTTGGTTTGGTAGTTGAGGT+GGG 0.430098 8_3:+15208327 None:intergenic
GTTGGGTCTATAAGGTTGAC+TGG 0.430997 8_3:+15208376 None:intergenic
AAAAGCGAGTTTCTCTAATC+AGG 0.431002 8_3:-15207146 Msa1278710:CDS
TTGGAGATATAAAGTTGGTT+TGG 0.431947 8_3:+15208314 None:intergenic
CTATAAGGTTGACTGGGTTG+TGG 0.439875 8_3:+15208383 None:intergenic
TTGACATGAAGATTTCTGAA+TGG 0.452845 8_3:+15206912 None:intergenic
GACAACACCATGGCAATGGC+AGG 0.453932 8_3:-15207055 Msa1278710:CDS
CCCTCCGCCCCTGTTTGGGG+TGG 0.454771 8_3:-15208448 Msa1278710:five_prime_UTR
TGCAGACACGGACAGTGATA+AGG 0.466791 8_3:-15207484 Msa1278710:intron
GAAGATAAGGTGATAGTTCA+GGG 0.467016 8_3:+15208483 None:intergenic
CCCACCCCAAACAGGGGCGG+AGG 0.474562 8_3:+15208447 None:intergenic
TATAAGGTTGACTGGGTTGT+GGG 0.477277 8_3:+15208384 None:intergenic
TAAAGTTGGTTTGGTAGTTG+AGG 0.479242 8_3:+15208323 None:intergenic
CTACCCTCCGCCCCTGTTTG+GGG 0.487205 8_3:-15208451 Msa1278710:five_prime_UTR
CAAGCTGCCGATCAAGCGCT+TGG 0.493218 8_3:-15207385 Msa1278710:CDS
GTAGTGTCTCTTTGAAGATA+AGG 0.494395 8_3:+15208470 None:intergenic
TGGTTTGGTAGTTGAGGTGG+GGG 0.496750 8_3:+15208329 None:intergenic
AGTTGGTTTGGTAGTTGAGG+TGG 0.500725 8_3:+15208326 None:intergenic
CTATGATAACAGAGAGCTCC+TGG 0.502060 8_3:+15207004 None:intergenic
CTTCGCTTGGGCCATTGAGA+AGG 0.506169 8_3:-15207115 Msa1278710:CDS
GTGATTTGGAGATATAAAGT+TGG 0.508285 8_3:+15208309 None:intergenic
TCCGTATTGTTGCAGAGATG+TGG 0.508624 8_3:-15207178 Msa1278710:intron
ATGGAATCCAAGCGCTTGAT+CGG 0.508867 8_3:+15207378 None:intergenic
CAGGAGAATTGACTTCGCTT+GGG 0.522026 8_3:-15207127 Msa1278710:CDS
TTCGATCCCCACCCCAAACA+GGG 0.526358 8_3:+15208440 None:intergenic
AATTAAGGAGTGTAATGAAA+AGG 0.529060 8_3:+15208407 None:intergenic
CCAGAAGATGGAAGAGGGCA+TGG 0.529484 8_3:-15207427 Msa1278710:exon
CATGGTGGAGAATTTGCAGT+TGG 0.529925 8_3:-15207031 Msa1278710:CDS
ACCATGCCCTGCCATTGCCA+TGG 0.542933 8_3:+15207048 None:intergenic
GCTTGATCGGCAGCTTGTCG+AGG 0.552952 8_3:+15207391 None:intergenic
ACCATGGCAATGGCAGGGCA+TGG 0.565803 8_3:-15207049 Msa1278710:CDS
CTTGATCGGCAGCTTGTCGA+GGG 0.576986 8_3:+15207392 None:intergenic
TTGGTTTGGTAGTTGAGGTG+GGG 0.587722 8_3:+15208328 None:intergenic
ATCCCCACCCCAAACAGGGG+CGG 0.591878 8_3:+15208444 None:intergenic
GAAGCAGAAGAAGAGCTCAA+AGG 0.592260 8_3:-15207079 Msa1278710:CDS
TTGGGTCTATAAGGTTGACT+GGG 0.609788 8_3:+15208377 None:intergenic
AGGAGACAACACCATGGCAA+TGG 0.615078 8_3:-15207059 Msa1278710:CDS
ATGGCAATGGCAGGGCATGG+TGG 0.618107 8_3:-15207046 Msa1278710:CDS
TCGATCCCCACCCCAAACAG+GGG 0.623559 8_3:+15208441 None:intergenic
TGAGGGGTATCCAAAACAGC+AGG 0.631590 8_3:+15208209 None:intergenic
ACAACACCATGGCAATGGCA+GGG 0.664781 8_3:-15207054 Msa1278710:CDS
TTGGGCCATTGAGAAGGACG+AGG 0.668552 8_3:-15207109 Msa1278710:CDS
GCTCAAAGGAGACAACACCA+TGG 0.768225 8_3:-15207065 Msa1278710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTGAATATTTTCTTAC+AGG + chr8_3:15207578-15207597 None:intergenic 15.0%
!! TATTCAAATTATATATTCGA+AGG - chr8_3:15207587-15207606 Msa1278710:intron 15.0%
!!! AACTATTTTATTATTCAGTT+AGG - chr8_3:15207779-15207798 Msa1278710:intron 15.0%
!!! AGGTTTTGTAAAAAATATAT+CGG - chr8_3:15207863-15207882 Msa1278710:intron 15.0%
!!! TAATTTGAATATTTTCTTAC+AGG + chr8_3:15207578-15207597 None:intergenic 15.0%
!! TATTCAAATTATATATTCGA+AGG - chr8_3:15207587-15207606 Msa1278710:intron 15.0%
!!! AACTATTTTATTATTCAGTT+AGG - chr8_3:15207779-15207798 Msa1278710:intron 15.0%
!!! AGGTTTTGTAAAAAATATAT+CGG - chr8_3:15207863-15207882 Msa1278710:intron 15.0%
!! AAAAATGGAAAATATCACAA+AGG + chr8_3:15207047-15207066 None:intergenic 20.0%
!! TTTCAAGCAGTTTATAAAAA+TGG + chr8_3:15207062-15207081 None:intergenic 20.0%
!!! ACTTGTCTTATTTTGATTTT+AGG - chr8_3:15207091-15207110 Msa1278710:CDS 20.0%
!! ATTAAATTAACACTTGAGAA+TGG + chr8_3:15207445-15207464 None:intergenic 20.0%
!! TTCTCAAGTGTTAATTTAAT+AGG - chr8_3:15207445-15207464 Msa1278710:five_prime_UTR 20.0%
!!! GCATAAAAAATTGATTCATT+GGG - chr8_3:15207467-15207486 Msa1278710:five_prime_UTR 20.0%
!! TATATATTCGAAGGATAATT+TGG - chr8_3:15207596-15207615 Msa1278710:intron 20.0%
!!! GGTTTTGTAAAAAATATATC+GGG - chr8_3:15207864-15207883 Msa1278710:intron 20.0%
!! ATACTAGTTGAGAAATAAAA+AGG + chr8_3:15208027-15208046 None:intergenic 20.0%
!! AAAAATGGAAAATATCACAA+AGG + chr8_3:15207047-15207066 None:intergenic 20.0%
!! TTTCAAGCAGTTTATAAAAA+TGG + chr8_3:15207062-15207081 None:intergenic 20.0%
!!! ACTTGTCTTATTTTGATTTT+AGG - chr8_3:15207091-15207110 Msa1278710:CDS 20.0%
!! ATTAAATTAACACTTGAGAA+TGG + chr8_3:15207445-15207464 None:intergenic 20.0%
!! TTCTCAAGTGTTAATTTAAT+AGG - chr8_3:15207445-15207464 Msa1278710:five_prime_UTR 20.0%
!!! GCATAAAAAATTGATTCATT+GGG - chr8_3:15207467-15207486 Msa1278710:five_prime_UTR 20.0%
!! TATATATTCGAAGGATAATT+TGG - chr8_3:15207596-15207615 Msa1278710:intron 20.0%
!!! GGTTTTGTAAAAAATATATC+GGG - chr8_3:15207864-15207883 Msa1278710:intron 20.0%
!! ATACTAGTTGAGAAATAAAA+AGG + chr8_3:15208027-15208046 None:intergenic 20.0%
!! AATTAAGGAGTGTAATGAAA+AGG + chr8_3:15206923-15206942 None:intergenic 25.0%
! GAGTGAAAAAACATTAAGTT+GGG + chr8_3:15206971-15206990 None:intergenic 25.0%
!! AGGCTAATTTTTGTTAATGT+TGG - chr8_3:15207201-15207220 Msa1278710:intron 25.0%
! TAAAATCGAAAGAAAGACAA+TGG + chr8_3:15207377-15207396 None:intergenic 25.0%
!! GGCATAAAAAATTGATTCAT+TGG - chr8_3:15207466-15207485 Msa1278710:five_prime_UTR 25.0%
!! ATGGATTATAACATATTCAC+TGG - chr8_3:15207519-15207538 Msa1278710:intron 25.0%
!! TTTTATTATTCAGTTAGGCT+TGG - chr8_3:15207784-15207803 Msa1278710:intron 25.0%
!! TTCATTTCCATTTTCTTCAA+TGG + chr8_3:15207971-15207990 None:intergenic 25.0%
! GGAAATGAAATCATAAAAAG+CGG + chr8_3:15208397-15208416 None:intergenic 25.0%
! GATAATTGCTTGAAGTTATT+TGG - chr8_3:15208478-15208497 Msa1278710:five_prime_UTR 25.0%
!! AGTTATTTGGTTTCTGAAAA+AGG - chr8_3:15208491-15208510 Msa1278710:five_prime_UTR 25.0%
!! AATTAAGGAGTGTAATGAAA+AGG + chr8_3:15206923-15206942 None:intergenic 25.0%
! GAGTGAAAAAACATTAAGTT+GGG + chr8_3:15206971-15206990 None:intergenic 25.0%
!! AGGCTAATTTTTGTTAATGT+TGG - chr8_3:15207201-15207220 Msa1278710:intron 25.0%
! TAAAATCGAAAGAAAGACAA+TGG + chr8_3:15207377-15207396 None:intergenic 25.0%
!! GGCATAAAAAATTGATTCAT+TGG - chr8_3:15207466-15207485 Msa1278710:five_prime_UTR 25.0%
!! ATGGATTATAACATATTCAC+TGG - chr8_3:15207519-15207538 Msa1278710:intron 25.0%
!! TTTTATTATTCAGTTAGGCT+TGG - chr8_3:15207784-15207803 Msa1278710:intron 25.0%
!! TTCATTTCCATTTTCTTCAA+TGG + chr8_3:15207971-15207990 None:intergenic 25.0%
! GGAAATGAAATCATAAAAAG+CGG + chr8_3:15208397-15208416 None:intergenic 25.0%
! GATAATTGCTTGAAGTTATT+TGG - chr8_3:15208478-15208497 Msa1278710:five_prime_UTR 25.0%
!! AGTTATTTGGTTTCTGAAAA+AGG - chr8_3:15208491-15208510 Msa1278710:five_prime_UTR 25.0%
!! AACATTAAGTTGGGTCTATA+AGG + chr8_3:15206962-15206981 None:intergenic 30.0%
CGAGTGAAAAAACATTAAGT+TGG + chr8_3:15206972-15206991 None:intergenic 30.0%
! TTGGAGATATAAAGTTGGTT+TGG + chr8_3:15207016-15207035 None:intergenic 30.0%
! GTGATTTGGAGATATAAAGT+TGG + chr8_3:15207021-15207040 None:intergenic 30.0%
! ACTTTTGTCAAATACTTGAG+GGG + chr8_3:15207137-15207156 None:intergenic 30.0%
! CACTTTTGTCAAATACTTGA+GGG + chr8_3:15207138-15207157 None:intergenic 30.0%
! CATTAACAAAAATTAGCCTC+AGG + chr8_3:15207200-15207219 None:intergenic 30.0%
! ATGAAGACTGATCTATGTTT+TGG - chr8_3:15207225-15207244 Msa1278710:intron 30.0%
TATTAATACTTTGACGTCAG+TGG - chr8_3:15207253-15207272 Msa1278710:intron 30.0%
ATATTCGAAGGATAATTTGG+TGG - chr8_3:15207599-15207618 Msa1278710:intron 30.0%
! AAACAAACTTTTCCAGAAGA+TGG - chr8_3:15207888-15207907 Msa1278710:intron 30.0%
TTGGATTCCATTGAAGAAAA+TGG - chr8_3:15207961-15207980 Msa1278710:intron 30.0%
CTAGTTGAGAAATAAAAAGG+AGG + chr8_3:15208024-15208043 None:intergenic 30.0%
TTGACATGAAGATTTCTGAA+TGG + chr8_3:15208418-15208437 None:intergenic 30.0%
!! AACATTAAGTTGGGTCTATA+AGG + chr8_3:15206962-15206981 None:intergenic 30.0%
CGAGTGAAAAAACATTAAGT+TGG + chr8_3:15206972-15206991 None:intergenic 30.0%
! TTGGAGATATAAAGTTGGTT+TGG + chr8_3:15207016-15207035 None:intergenic 30.0%
! GTGATTTGGAGATATAAAGT+TGG + chr8_3:15207021-15207040 None:intergenic 30.0%
! ACTTTTGTCAAATACTTGAG+GGG + chr8_3:15207137-15207156 None:intergenic 30.0%
! CACTTTTGTCAAATACTTGA+GGG + chr8_3:15207138-15207157 None:intergenic 30.0%
! CATTAACAAAAATTAGCCTC+AGG + chr8_3:15207200-15207219 None:intergenic 30.0%
! ATGAAGACTGATCTATGTTT+TGG - chr8_3:15207225-15207244 Msa1278710:intron 30.0%
TATTAATACTTTGACGTCAG+TGG - chr8_3:15207253-15207272 Msa1278710:intron 30.0%
ATATTCGAAGGATAATTTGG+TGG - chr8_3:15207599-15207618 Msa1278710:intron 30.0%
! AAACAAACTTTTCCAGAAGA+TGG - chr8_3:15207888-15207907 Msa1278710:intron 30.0%
TTGGATTCCATTGAAGAAAA+TGG - chr8_3:15207961-15207980 Msa1278710:intron 30.0%
CTAGTTGAGAAATAAAAAGG+AGG + chr8_3:15208024-15208043 None:intergenic 30.0%
TTGACATGAAGATTTCTGAA+TGG + chr8_3:15208418-15208437 None:intergenic 30.0%
TAGTTCAGGGACAAAATTGA+TGG + chr8_3:15206834-15206853 None:intergenic 35.0%
GAAGATAAGGTGATAGTTCA+GGG + chr8_3:15206847-15206866 None:intergenic 35.0%
TGAAGATAAGGTGATAGTTC+AGG + chr8_3:15206848-15206867 None:intergenic 35.0%
! GTAGTGTCTCTTTGAAGATA+AGG + chr8_3:15206860-15206879 None:intergenic 35.0%
!! TAAAGTTGGTTTGGTAGTTG+AGG + chr8_3:15207007-15207026 None:intergenic 35.0%
TATCACAAAGGTGAGTGATT+TGG + chr8_3:15207035-15207054 None:intergenic 35.0%
! GCACTTTTGTCAAATACTTG+AGG + chr8_3:15207139-15207158 None:intergenic 35.0%
! TGATGTGAAAATGCTGTTTG+TGG - chr8_3:15207348-15207367 Msa1278710:CDS 35.0%
GTTGAGAAATAAAAAGGAGG+AGG + chr8_3:15208021-15208040 None:intergenic 35.0%
GCAAGCGCAAATAGAAATTA+GGG + chr8_3:15208115-15208134 None:intergenic 35.0%
AGCAAGCGCAAATAGAAATT+AGG + chr8_3:15208116-15208135 None:intergenic 35.0%
AAAAGCGAGTTTCTCTAATC+AGG - chr8_3:15208181-15208200 Msa1278710:five_prime_UTR 35.0%
! CATTTTTGCGAACAGATATG+CGG + chr8_3:15208455-15208474 None:intergenic 35.0%
TAGTTCAGGGACAAAATTGA+TGG + chr8_3:15206834-15206853 None:intergenic 35.0%
GAAGATAAGGTGATAGTTCA+GGG + chr8_3:15206847-15206866 None:intergenic 35.0%
TGAAGATAAGGTGATAGTTC+AGG + chr8_3:15206848-15206867 None:intergenic 35.0%
! GTAGTGTCTCTTTGAAGATA+AGG + chr8_3:15206860-15206879 None:intergenic 35.0%
!! TAAAGTTGGTTTGGTAGTTG+AGG + chr8_3:15207007-15207026 None:intergenic 35.0%
TATCACAAAGGTGAGTGATT+TGG + chr8_3:15207035-15207054 None:intergenic 35.0%
! GCACTTTTGTCAAATACTTG+AGG + chr8_3:15207139-15207158 None:intergenic 35.0%
! TGATGTGAAAATGCTGTTTG+TGG - chr8_3:15207348-15207367 Msa1278710:CDS 35.0%
GTTGAGAAATAAAAAGGAGG+AGG + chr8_3:15208021-15208040 None:intergenic 35.0%
GCAAGCGCAAATAGAAATTA+GGG + chr8_3:15208115-15208134 None:intergenic 35.0%
AGCAAGCGCAAATAGAAATT+AGG + chr8_3:15208116-15208135 None:intergenic 35.0%
AAAAGCGAGTTTCTCTAATC+AGG - chr8_3:15208181-15208200 Msa1278710:five_prime_UTR 35.0%
! CATTTTTGCGAACAGATATG+CGG + chr8_3:15208455-15208474 None:intergenic 35.0%
TATAAGGTTGACTGGGTTGT+GGG + chr8_3:15206946-15206965 None:intergenic 40.0%
TTGGGTCTATAAGGTTGACT+GGG + chr8_3:15206953-15206972 None:intergenic 40.0%
!!! TTTAGGAGTACCTGCTGTTT+TGG - chr8_3:15207108-15207127 Msa1278710:CDS 40.0%
!! AACCATTTTGTTTGCACCTG+AGG - chr8_3:15207181-15207200 Msa1278710:intron 40.0%
TTGTTCTCTTCTTGCATGAC+TGG - chr8_3:15207416-15207435 Msa1278710:CDS 40.0%
! TTCACTGGTTAACTGCTAAC+AGG - chr8_3:15207534-15207553 Msa1278710:intron 40.0%
TTGGACTTAAAGCTACTGTC+TGG - chr8_3:15207803-15207822 Msa1278710:intron 40.0%
! ACTTTTCCAGAAGATGGAAG+AGG - chr8_3:15207894-15207913 Msa1278710:intron 40.0%
! CTTTTCCAGAAGATGGAAGA+GGG - chr8_3:15207895-15207914 Msa1278710:intron 40.0%
GAGAGGAAGAAGCTTACAAA+GGG + chr8_3:15207999-15208018 None:intergenic 40.0%
GAAATAAAAAGGAGGAGGAG+AGG + chr8_3:15208016-15208035 None:intergenic 40.0%
ACGAGAGATAGCATAACTCT+AGG + chr8_3:15208080-15208099 None:intergenic 40.0%
TCCACATCTCTGCAACAATA+CGG + chr8_3:15208153-15208172 None:intergenic 40.0%
TATAAGGTTGACTGGGTTGT+GGG + chr8_3:15206946-15206965 None:intergenic 40.0%
TTGGGTCTATAAGGTTGACT+GGG + chr8_3:15206953-15206972 None:intergenic 40.0%
!!! TTTAGGAGTACCTGCTGTTT+TGG - chr8_3:15207108-15207127 Msa1278710:CDS 40.0%
!! AACCATTTTGTTTGCACCTG+AGG - chr8_3:15207181-15207200 Msa1278710:intron 40.0%
TTGTTCTCTTCTTGCATGAC+TGG - chr8_3:15207416-15207435 Msa1278710:CDS 40.0%
! TTCACTGGTTAACTGCTAAC+AGG - chr8_3:15207534-15207553 Msa1278710:intron 40.0%
TTGGACTTAAAGCTACTGTC+TGG - chr8_3:15207803-15207822 Msa1278710:intron 40.0%
! ACTTTTCCAGAAGATGGAAG+AGG - chr8_3:15207894-15207913 Msa1278710:intron 40.0%
! CTTTTCCAGAAGATGGAAGA+GGG - chr8_3:15207895-15207914 Msa1278710:intron 40.0%
GAGAGGAAGAAGCTTACAAA+GGG + chr8_3:15207999-15208018 None:intergenic 40.0%
GAAATAAAAAGGAGGAGGAG+AGG + chr8_3:15208016-15208035 None:intergenic 40.0%
ACGAGAGATAGCATAACTCT+AGG + chr8_3:15208080-15208099 None:intergenic 40.0%
TCCACATCTCTGCAACAATA+CGG + chr8_3:15208153-15208172 None:intergenic 40.0%
TGACTGGGTTGTGGGAATTA+AGG + chr8_3:15206938-15206957 None:intergenic 45.0%
CTATAAGGTTGACTGGGTTG+TGG + chr8_3:15206947-15206966 None:intergenic 45.0%
GTTGGGTCTATAAGGTTGAC+TGG + chr8_3:15206954-15206973 None:intergenic 45.0%
!! TTGGTTTGGTAGTTGAGGTG+GGG + chr8_3:15207002-15207021 None:intergenic 45.0%
!! GTTGGTTTGGTAGTTGAGGT+GGG + chr8_3:15207003-15207022 None:intergenic 45.0%
!! AGTTGGTTTGGTAGTTGAGG+TGG + chr8_3:15207004-15207023 None:intergenic 45.0%
AGCCTCAGGTGCAAACAAAA+TGG + chr8_3:15207186-15207205 None:intergenic 45.0%
ACTTTGACGTCAGTGGTACT+CGG - chr8_3:15207260-15207279 Msa1278710:intron 45.0%
TACGTGCATTTGCATGCTGA+TGG - chr8_3:15207500-15207519 Msa1278710:intron 45.0%
CCGTGTCTGCAAGAAAATGT+GGG + chr8_3:15207834-15207853 None:intergenic 45.0%
! CCCACATTTTCTTGCAGACA+CGG - chr8_3:15207831-15207850 Msa1278710:intron 45.0%
TCCGTGTCTGCAAGAAAATG+TGG + chr8_3:15207835-15207854 None:intergenic 45.0%
ATGGAATCCAAGCGCTTGAT+CGG + chr8_3:15207952-15207971 None:intergenic 45.0%
GGAGAGGAAGAAGCTTACAA+AGG + chr8_3:15208000-15208019 None:intergenic 45.0%
CATCTCTGCAACAATACGGA+GGG + chr8_3:15208149-15208168 None:intergenic 45.0%
ACATCTCTGCAACAATACGG+AGG + chr8_3:15208150-15208169 None:intergenic 45.0%
TCCGTATTGTTGCAGAGATG+TGG - chr8_3:15208149-15208168 Msa1278710:five_prime_UTR 45.0%
! TCAGGAGAATTGACTTCGCT+TGG - chr8_3:15208199-15208218 Msa1278710:five_prime_UTR 45.0%
! CAGGAGAATTGACTTCGCTT+GGG - chr8_3:15208200-15208219 Msa1278710:five_prime_UTR 45.0%
! CTTTTCCTCGTCCTTCTCAA+TGG + chr8_3:15208226-15208245 None:intergenic 45.0%
GAAGCAGAAGAAGAGCTCAA+AGG - chr8_3:15208248-15208267 Msa1278710:five_prime_UTR 45.0%
! CATGGTGGAGAATTTGCAGT+TGG - chr8_3:15208296-15208315 Msa1278710:five_prime_UTR 45.0%
CTATGATAACAGAGAGCTCC+TGG + chr8_3:15208326-15208345 None:intergenic 45.0%
TGACTGGGTTGTGGGAATTA+AGG + chr8_3:15206938-15206957 None:intergenic 45.0%
CTATAAGGTTGACTGGGTTG+TGG + chr8_3:15206947-15206966 None:intergenic 45.0%
GTTGGGTCTATAAGGTTGAC+TGG + chr8_3:15206954-15206973 None:intergenic 45.0%
!! TTGGTTTGGTAGTTGAGGTG+GGG + chr8_3:15207002-15207021 None:intergenic 45.0%
!! GTTGGTTTGGTAGTTGAGGT+GGG + chr8_3:15207003-15207022 None:intergenic 45.0%
!! AGTTGGTTTGGTAGTTGAGG+TGG + chr8_3:15207004-15207023 None:intergenic 45.0%
AGCCTCAGGTGCAAACAAAA+TGG + chr8_3:15207186-15207205 None:intergenic 45.0%
ACTTTGACGTCAGTGGTACT+CGG - chr8_3:15207260-15207279 Msa1278710:intron 45.0%
TACGTGCATTTGCATGCTGA+TGG - chr8_3:15207500-15207519 Msa1278710:intron 45.0%
CCGTGTCTGCAAGAAAATGT+GGG + chr8_3:15207834-15207853 None:intergenic 45.0%
! CCCACATTTTCTTGCAGACA+CGG - chr8_3:15207831-15207850 Msa1278710:intron 45.0%
TCCGTGTCTGCAAGAAAATG+TGG + chr8_3:15207835-15207854 None:intergenic 45.0%
ATGGAATCCAAGCGCTTGAT+CGG + chr8_3:15207952-15207971 None:intergenic 45.0%
GGAGAGGAAGAAGCTTACAA+AGG + chr8_3:15208000-15208019 None:intergenic 45.0%
CATCTCTGCAACAATACGGA+GGG + chr8_3:15208149-15208168 None:intergenic 45.0%
ACATCTCTGCAACAATACGG+AGG + chr8_3:15208150-15208169 None:intergenic 45.0%
TCCGTATTGTTGCAGAGATG+TGG - chr8_3:15208149-15208168 Msa1278710:five_prime_UTR 45.0%
! TCAGGAGAATTGACTTCGCT+TGG - chr8_3:15208199-15208218 Msa1278710:five_prime_UTR 45.0%
! CAGGAGAATTGACTTCGCTT+GGG - chr8_3:15208200-15208219 Msa1278710:five_prime_UTR 45.0%
! CTTTTCCTCGTCCTTCTCAA+TGG + chr8_3:15208226-15208245 None:intergenic 45.0%
GAAGCAGAAGAAGAGCTCAA+AGG - chr8_3:15208248-15208267 Msa1278710:five_prime_UTR 45.0%
! CATGGTGGAGAATTTGCAGT+TGG - chr8_3:15208296-15208315 Msa1278710:five_prime_UTR 45.0%
CTATGATAACAGAGAGCTCC+TGG + chr8_3:15208326-15208345 None:intergenic 45.0%
!!! TAAAATAAAAATTCATTTTA+GGG + chr8_3:15207633-15207652 None:intergenic 5.0%
!!! TTAAAATAAAAATTCATTTT+AGG + chr8_3:15207634-15207653 None:intergenic 5.0%
!!! TAAAATAAAAATTCATTTTA+GGG + chr8_3:15207633-15207652 None:intergenic 5.0%
!!! TTAAAATAAAAATTCATTTT+AGG + chr8_3:15207634-15207653 None:intergenic 5.0%
!! TGGTTTGGTAGTTGAGGTGG+GGG + chr8_3:15207001-15207020 None:intergenic 50.0%
!! TGAGGGGTATCCAAAACAGC+AGG + chr8_3:15207121-15207140 None:intergenic 50.0%
! CTTGGTGTGGCAATCCTTTG+TGG - chr8_3:15207285-15207304 Msa1278710:intron 50.0%
GTGTGGCAATCCTTTGTGGA+TGG - chr8_3:15207289-15207308 Msa1278710:intron 50.0%
GGCAATCCTTTGTGGATGGT+TGG - chr8_3:15207293-15207312 Msa1278710:intron 50.0%
AGCAGTCCAACCATCCACAA+AGG + chr8_3:15207302-15207321 None:intergenic 50.0%
TGCAGACACGGACAGTGATA+AGG - chr8_3:15207843-15207862 Msa1278710:intron 50.0%
TAGAGAACCTGTGCAACGCT+CGG - chr8_3:15208046-15208065 Msa1278710:intron 50.0%
GCTCAAAGGAGACAACACCA+TGG - chr8_3:15208262-15208281 Msa1278710:five_prime_UTR 50.0%
AGGAGACAACACCATGGCAA+TGG - chr8_3:15208268-15208287 Msa1278710:five_prime_UTR 50.0%
ACAACACCATGGCAATGGCA+GGG - chr8_3:15208273-15208292 Msa1278710:five_prime_UTR 50.0%
! GAATTTGCAGTTGGCTCACC+AGG - chr8_3:15208305-15208324 Msa1278710:five_prime_UTR 50.0%
!! TGGTTTGGTAGTTGAGGTGG+GGG + chr8_3:15207001-15207020 None:intergenic 50.0%
!! TGAGGGGTATCCAAAACAGC+AGG + chr8_3:15207121-15207140 None:intergenic 50.0%
! CTTGGTGTGGCAATCCTTTG+TGG - chr8_3:15207285-15207304 Msa1278710:intron 50.0%
GTGTGGCAATCCTTTGTGGA+TGG - chr8_3:15207289-15207308 Msa1278710:intron 50.0%
GGCAATCCTTTGTGGATGGT+TGG - chr8_3:15207293-15207312 Msa1278710:intron 50.0%
AGCAGTCCAACCATCCACAA+AGG + chr8_3:15207302-15207321 None:intergenic 50.0%
TGCAGACACGGACAGTGATA+AGG - chr8_3:15207843-15207862 Msa1278710:intron 50.0%
TAGAGAACCTGTGCAACGCT+CGG - chr8_3:15208046-15208065 Msa1278710:intron 50.0%
GCTCAAAGGAGACAACACCA+TGG - chr8_3:15208262-15208281 Msa1278710:five_prime_UTR 50.0%
AGGAGACAACACCATGGCAA+TGG - chr8_3:15208268-15208287 Msa1278710:five_prime_UTR 50.0%
ACAACACCATGGCAATGGCA+GGG - chr8_3:15208273-15208292 Msa1278710:five_prime_UTR 50.0%
! GAATTTGCAGTTGGCTCACC+AGG - chr8_3:15208305-15208324 Msa1278710:five_prime_UTR 50.0%
TTCGATCCCCACCCCAAACA+GGG + chr8_3:15206890-15206909 None:intergenic 55.0%
CCATGCCCTCTTCCATCTTC+TGG + chr8_3:15207903-15207922 None:intergenic 55.0%
CCAGAAGATGGAAGAGGGCA+TGG - chr8_3:15207900-15207919 Msa1278710:intron 55.0%
CTTGATCGGCAGCTTGTCGA+GGG + chr8_3:15207938-15207957 None:intergenic 55.0%
AGAGAACCTGTGCAACGCTC+GGG - chr8_3:15208047-15208066 Msa1278710:intron 55.0%
!! TTTTTCCCCGAGCGTTGCAC+AGG + chr8_3:15208056-15208075 None:intergenic 55.0%
CTTCGCTTGGGCCATTGAGA+AGG - chr8_3:15208212-15208231 Msa1278710:five_prime_UTR 55.0%
TTGGGCCATTGAGAAGGACG+AGG - chr8_3:15208218-15208237 Msa1278710:five_prime_UTR 55.0%
GACAACACCATGGCAATGGC+AGG - chr8_3:15208272-15208291 Msa1278710:five_prime_UTR 55.0%
TTCGATCCCCACCCCAAACA+GGG + chr8_3:15206890-15206909 None:intergenic 55.0%
CCATGCCCTCTTCCATCTTC+TGG + chr8_3:15207903-15207922 None:intergenic 55.0%
CCAGAAGATGGAAGAGGGCA+TGG - chr8_3:15207900-15207919 Msa1278710:intron 55.0%
CTTGATCGGCAGCTTGTCGA+GGG + chr8_3:15207938-15207957 None:intergenic 55.0%
AGAGAACCTGTGCAACGCTC+GGG - chr8_3:15208047-15208066 Msa1278710:intron 55.0%
!! TTTTTCCCCGAGCGTTGCAC+AGG + chr8_3:15208056-15208075 None:intergenic 55.0%
CTTCGCTTGGGCCATTGAGA+AGG - chr8_3:15208212-15208231 Msa1278710:five_prime_UTR 55.0%
TTGGGCCATTGAGAAGGACG+AGG - chr8_3:15208218-15208237 Msa1278710:five_prime_UTR 55.0%
GACAACACCATGGCAATGGC+AGG - chr8_3:15208272-15208291 Msa1278710:five_prime_UTR 55.0%
ACTACCCTCCGCCCCTGTTT+GGG - chr8_3:15206875-15206894 Msa1278710:three_prime_UTR 60.0%
TCGATCCCCACCCCAAACAG+GGG + chr8_3:15206889-15206908 None:intergenic 60.0%
CTTCGATCCCCACCCCAAAC+AGG + chr8_3:15206891-15206910 None:intergenic 60.0%
!! CGTCAGTGGTACTCGGTGCT+TGG - chr8_3:15207267-15207286 Msa1278710:intron 60.0%
!! GTGGTACTCGGTGCTTGGTG+TGG - chr8_3:15207272-15207291 Msa1278710:intron 60.0%
GCTTGATCGGCAGCTTGTCG+AGG + chr8_3:15207939-15207958 None:intergenic 60.0%
CAAGCTGCCGATCAAGCGCT+TGG - chr8_3:15207942-15207961 Msa1278710:intron 60.0%
GAGAACCTGTGCAACGCTCG+GGG - chr8_3:15208048-15208067 Msa1278710:intron 60.0%
ACCATGGCAATGGCAGGGCA+TGG - chr8_3:15208278-15208297 Msa1278710:five_prime_UTR 60.0%
ACCATGCCCTGCCATTGCCA+TGG + chr8_3:15208282-15208301 None:intergenic 60.0%
ATGGCAATGGCAGGGCATGG+TGG - chr8_3:15208281-15208300 Msa1278710:five_prime_UTR 60.0%
ACTACCCTCCGCCCCTGTTT+GGG - chr8_3:15206875-15206894 Msa1278710:three_prime_UTR 60.0%
TCGATCCCCACCCCAAACAG+GGG + chr8_3:15206889-15206908 None:intergenic 60.0%
CTTCGATCCCCACCCCAAAC+AGG + chr8_3:15206891-15206910 None:intergenic 60.0%
!! CGTCAGTGGTACTCGGTGCT+TGG - chr8_3:15207267-15207286 Msa1278710:intron 60.0%
!! GTGGTACTCGGTGCTTGGTG+TGG - chr8_3:15207272-15207291 Msa1278710:intron 60.0%
GCTTGATCGGCAGCTTGTCG+AGG + chr8_3:15207939-15207958 None:intergenic 60.0%
CAAGCTGCCGATCAAGCGCT+TGG - chr8_3:15207942-15207961 Msa1278710:intron 60.0%
GAGAACCTGTGCAACGCTCG+GGG - chr8_3:15208048-15208067 Msa1278710:intron 60.0%
ACCATGGCAATGGCAGGGCA+TGG - chr8_3:15208278-15208297 Msa1278710:five_prime_UTR 60.0%
ACCATGCCCTGCCATTGCCA+TGG + chr8_3:15208282-15208301 None:intergenic 60.0%
ATGGCAATGGCAGGGCATGG+TGG - chr8_3:15208281-15208300 Msa1278710:five_prime_UTR 60.0%
CACTACCCTCCGCCCCTGTT+TGG - chr8_3:15206874-15206893 Msa1278710:three_prime_UTR 65.0%
CTACCCTCCGCCCCTGTTTG+GGG - chr8_3:15206876-15206895 Msa1278710:three_prime_UTR 65.0%
ATCCCCACCCCAAACAGGGG+CGG + chr8_3:15206886-15206905 None:intergenic 65.0%
CACTACCCTCCGCCCCTGTT+TGG - chr8_3:15206874-15206893 Msa1278710:three_prime_UTR 65.0%
CTACCCTCCGCCCCTGTTTG+GGG - chr8_3:15206876-15206895 Msa1278710:three_prime_UTR 65.0%
ATCCCCACCCCAAACAGGGG+CGG + chr8_3:15206886-15206905 None:intergenic 65.0%
CCACCCCAAACAGGGGCGGA+GGG + chr8_3:15206882-15206901 None:intergenic 70.0%
! CCTCCGCCCCTGTTTGGGGT+GGG - chr8_3:15206880-15206899 Msa1278710:three_prime_UTR 70.0%
! CTCCGCCCCTGTTTGGGGTG+GGG - chr8_3:15206881-15206900 Msa1278710:three_prime_UTR 70.0%
CCACCCCAAACAGGGGCGGA+GGG + chr8_3:15206882-15206901 None:intergenic 70.0%
! CCTCCGCCCCTGTTTGGGGT+GGG - chr8_3:15206880-15206899 Msa1278710:three_prime_UTR 70.0%
! CTCCGCCCCTGTTTGGGGTG+GGG - chr8_3:15206881-15206900 Msa1278710:three_prime_UTR 70.0%
CCCTCCGCCCCTGTTTGGGG+TGG - chr8_3:15206879-15206898 Msa1278710:three_prime_UTR 75.0%
CCCACCCCAAACAGGGGCGG+AGG + chr8_3:15206883-15206902 None:intergenic 75.0%
CCCTCCGCCCCTGTTTGGGG+TGG - chr8_3:15206879-15206898 Msa1278710:three_prime_UTR 75.0%
CCCACCCCAAACAGGGGCGG+AGG + chr8_3:15206883-15206902 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 15206824 15208525 15206824 ID=Msa1278710;Name=Msa1278710
chr8_3 mRNA 15206824 15208525 15206824 ID=Msa1278710-mRNA-1;Parent=Msa1278710;Name=Msa1278710-mRNA-1;_AED=0.00;_eAED=0.00;_QI=458|1|1|1|0.5|0.33|3|136|106
chr8_3 exon 15206824 15207185 15206824 ID=Msa1278710-mRNA-1:exon:5892;Parent=Msa1278710-mRNA-1
chr8_3 exon 15207348 15207501 15207348 ID=Msa1278710-mRNA-1:exon:5891;Parent=Msa1278710-mRNA-1
chr8_3 exon 15208127 15208525 15208127 ID=Msa1278710-mRNA-1:exon:5890;Parent=Msa1278710-mRNA-1
chr8_3 five_prime_UTR 15208127 15208525 15208127 ID=Msa1278710-mRNA-1:five_prime_utr;Parent=Msa1278710-mRNA-1
chr8_3 five_prime_UTR 15207443 15207501 15207443 ID=Msa1278710-mRNA-1:five_prime_utr;Parent=Msa1278710-mRNA-1
chr8_3 CDS 15207348 15207442 15207348 ID=Msa1278710-mRNA-1:cds;Parent=Msa1278710-mRNA-1
chr8_3 CDS 15206960 15207185 15206960 ID=Msa1278710-mRNA-1:cds;Parent=Msa1278710-mRNA-1
chr8_3 three_prime_UTR 15206824 15206959 15206824 ID=Msa1278710-mRNA-1:three_prime_utr;Parent=Msa1278710-mRNA-1
Gene Sequence

>Msa1278710

ATGGAAGAGGGCATGGAGTTGCAACTCTCCCTCGACAAGCTGCCGATCAAGCGCTTGGATTCCATTGAAGAAAATGGAAATGAACGATTCCCTTTAGATGTGGATTACGATGAAAAGCGAGTTTCTCTAATCAGGAGAATTGACTTCGCTTGGGCCATTGAGAAGGACGAGGAAAAGAAGAAGCAGAAGAAGAGCTCAAAGGAGACAACACCATGGCAATGGCAGGGCATGGTGGAGAATTTGCAGTTGGCTCACCAGGAGCTCTCTGTTATCATAGATTTCATCAACACTGTTAGTAGTGCATGTTTCTTTCTACAATGA
Protein sequence

>Msa1278710

MEEGMELQLSLDKLPIKRLDSIEENGNERFPLDVDYDEKRVSLIRRIDFAWAIEKDEEKKKQKKSSKETTPWQWQGMVENLQLAHQELSVIIDFINTVSSACFFLQ*