AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1296900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1296900 MtrunA17_Chr3g0138401 87.500 96 9 1 96 188 321 416 1.30e-49 165
Msa1296900 MtrunA17_Chr7g0265591 100.000 30 0 0 32 61 262 291 1.49e-13 68.2
Msa1296900 MtrunA17_Chr1g0179541 100.000 30 0 0 32 61 246 275 1.80e-13 67.8
Msa1296900 MtrunA17_Chr1g0198231 90.323 31 3 0 61 91 291 321 2.41e-13 67.4
Msa1296900 MtrunA17_Chr8g0367631 87.097 31 4 0 61 91 281 311 1.11e-12 65.1
Msa1296900 MtrunA17_Chr1g0197911 90.000 30 3 0 32 61 261 290 1.12e-11 62.8
Msa1296900 MtrunA17_Chr2g0278901 77.419 31 7 0 61 91 284 314 5.53e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1296900 AT3G52990.2 100.000 30 0 0 32 61 209 238 2.24e-13 67.8
Msa1296900 AT3G52990.1 100.000 30 0 0 32 61 262 291 2.42e-13 67.8
Msa1296900 AT2G36580.1 100.000 30 0 0 32 61 262 291 2.95e-13 67.8

Find 58 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTTGTCTCGTGGGAATT+TGG 0.117949 8_3:-56723870 Msa1296900:CDS
TGCTTGAACCGCCGGATTTC+TGG 0.125938 8_3:+56721111 None:intergenic
TCTAAACCGGTCACCTTATC+AGG 0.216944 8_3:-56720805 Msa1296900:three_prime_UTR
ACCAGCTTTGCAGTGGATTT+AGG 0.260311 8_3:+56721785 None:intergenic
CTGTGTCTAGGTGCAGGGTC+TGG 0.269441 8_3:-56724243 Msa1296900:intron
CTTGTAGAATCTCATCAAAA+TGG 0.282372 8_3:+56723906 None:intergenic
ATTGAATGTACTGCAGAGTA+TGG 0.297645 8_3:+56722267 None:intergenic
GCATACCGTGTAAGCATATA+TGG 0.315006 8_3:+56722234 None:intergenic
ATCTTGTCTCGTGGGAATTT+GGG 0.330103 8_3:-56723869 Msa1296900:CDS
TTTGCTTATTCCTCTTTGAT+AGG 0.334638 8_3:-56724179 Msa1296900:CDS
GTCTACAGGTTTGTTATGAT+TGG 0.341574 8_3:-56721853 Msa1296900:intron
CATAGTGGAGAAAATGAAAC+AGG 0.343306 8_3:-56721746 Msa1296900:CDS
CAGAAATAAGAGAAAGCTCT+TGG 0.383667 8_3:+56721004 None:intergenic
TTCTATTGCAGTTTCTAAAC+CGG 0.384539 8_3:-56720818 Msa1296900:three_prime_UTR
TGTGTCTAGGTGCAGGGTCT+GGG 0.392512 8_3:-56724242 Msa1296900:intron
ACCTAAATCCACTGCAAAGC+TGG 0.415322 8_3:-56721786 Msa1296900:CDS
TGCTAAAGATACCAGAAATC+CGG 0.424285 8_3:-56721122 Msa1296900:CDS
TCCTGCTGTGTCTAGGTGCA+GGG 0.434688 8_3:-56724248 Msa1296900:intron
CATTGATCTTCCACCAGAAA+AGG 0.440845 8_3:-56723846 Msa1296900:intron
AGAAGATGCTTGACCTGATA+AGG 0.443032 8_3:+56720792 None:intergenic
TTGTAGAATCTCATCAAAAT+GGG 0.469141 8_3:+56723907 None:intergenic
GGAGTAGATACACGATCAAC+AGG 0.476638 8_3:+56721653 None:intergenic
TTAAACATGTTGGCATTTGT+TGG 0.478202 8_3:-56724213 Msa1296900:CDS
ACTGCAAAGATGGTGGATCT+TGG 0.479375 8_3:-56721823 Msa1296900:CDS
GTGAGAAAATTTGACAAAAG+AGG 0.485887 8_3:+56720962 None:intergenic
GGATCTGCTGCTTGAACCGC+CGG 0.490647 8_3:+56721103 None:intergenic
AGAAATAAGAGAAAGCTCTT+GGG 0.490918 8_3:+56721005 None:intergenic
AGTAATCCTCAAGAGCTTCA+TGG 0.497229 8_3:-56721716 Msa1296900:CDS
ATGTTTCTGTAATCAATGGC+AGG 0.504577 8_3:-56724968 None:intergenic
GAGTAGGATGATTGTTAAAG+AGG 0.509074 8_3:-56720895 Msa1296900:three_prime_UTR
AACTTCCCATGAAGCTCTTG+AGG 0.518605 8_3:+56721710 None:intergenic
ATTATGTGGGGAGCAAGAGT+AGG 0.524042 8_3:-56720911 Msa1296900:three_prime_UTR
GTAATCCTCAAGAGCTTCAT+GGG 0.525373 8_3:-56721715 Msa1296900:CDS
AATAACAACCTTACGAATGT+TGG 0.526846 8_3:-56720757 Msa1296900:three_prime_UTR
TGGGTTAATATTAAACATGT+TGG 0.527799 8_3:-56724223 Msa1296900:CDS
ATCATTGACCAGCTTTGCAG+TGG 0.539773 8_3:+56721778 None:intergenic
CGCAGTTCTAGCCGTGCCGT+TGG 0.541509 8_3:-56721677 Msa1296900:CDS
TTATTCCTCTTTGATAGGAG+TGG 0.550259 8_3:-56724174 Msa1296900:CDS
TGGAGCAACTACTGCAAAGA+TGG 0.553115 8_3:-56721833 Msa1296900:CDS
TCTACTTACCAACATTCGTA+AGG 0.553647 8_3:+56720749 None:intergenic
CGGTTCCATATATGCTTACA+CGG 0.554203 8_3:-56722239 Msa1296900:intron
GCTTGACCTGATAAGGTGAC+CGG 0.558454 8_3:+56720799 None:intergenic
ATGGAATCATCTTGTCTCGT+GGG 0.568136 8_3:-56723877 Msa1296900:CDS
AATGATAGTAACTTGCATAG+TGG 0.579416 8_3:-56721761 Msa1296900:CDS
GAGATTCTACAAGAAGCTGA+TGG 0.579505 8_3:-56723896 Msa1296900:CDS
TGCAGTACATTCAATAATGA+CGG 0.593718 8_3:-56722259 Msa1296900:CDS
GATCGTGTATCTACTCCAGC+CGG 0.597972 8_3:-56721647 Msa1296900:intron
TACACGATCAACAGGTCCAA+CGG 0.603243 8_3:+56721661 None:intergenic
CCACGCCACTCCTATCAAAG+AGG 0.603868 8_3:+56724169 None:intergenic
TAATTGATGTATCTGTTAGA+CGG 0.604298 8_3:+56720871 None:intergenic
GATCAACAGGTCCAACGGCA+CGG 0.605689 8_3:+56721666 None:intergenic
TGTATTGTAGGGGCACATCA+AGG 0.610537 8_3:-56721154 Msa1296900:intron
GATGGAATCATCTTGTCTCG+TGG 0.615425 8_3:-56723878 Msa1296900:CDS
AGCAACTACTGCAAAGATGG+TGG 0.619072 8_3:-56721830 Msa1296900:CDS
CCTCTTTGATAGGAGTGGCG+TGG 0.625318 8_3:-56724169 Msa1296900:intron
TTGAATGTACTGCAGAGTAT+GGG 0.646955 8_3:+56722268 None:intergenic
TAAAGATACCAGAAATCCGG+CGG 0.721164 8_3:-56721119 Msa1296900:CDS
ATAGTGGAGAAAATGAAACA+GGG 0.722474 8_3:-56721745 Msa1296900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAGAAAAATTTGATTTTTT+TGG - chr8_3:56721065-56721084 Msa1296900:exon 10.0%
!! TACTTAATAATTCTTAAAAT+GGG - chr8_3:56721915-56721934 Msa1296900:intron 10.0%
!!! ACATAATCTTTTTTTTTTTA+AGG + chr8_3:56724722-56724741 None:intergenic 10.0%
!! TTAAAAAAAAAAAGATTATG+TGG - chr8_3:56724721-56724740 Msa1296900:intron 10.0%
!! TAAAAAAAAAAAGATTATGT+GGG - chr8_3:56724722-56724741 Msa1296900:intron 10.0%
!!! AAATTTGATTTTTTTGGATT+TGG - chr8_3:56721071-56721090 Msa1296900:exon 15.0%
!!! TTTATATATCTGTTCTTTTT+AGG - chr8_3:56721710-56721729 Msa1296900:CDS 15.0%
!!! TTATATATCTGTTCTTTTTA+GGG - chr8_3:56721711-56721730 Msa1296900:CDS 15.0%
!! GTACTTAATAATTCTTAAAA+TGG - chr8_3:56721914-56721933 Msa1296900:intron 15.0%
!! ACTTAATAATTCTTAAAATG+GGG - chr8_3:56721916-56721935 Msa1296900:intron 15.0%
!!! AGATTTTTACTCTTTAAAAT+AGG + chr8_3:56722015-56722034 None:intergenic 15.0%
!!! TATCTAATCTAAGAATTTTT+AGG - chr8_3:56722945-56722964 Msa1296900:intron 15.0%
!! TTCTAAATTCATTAGAATAT+TGG - chr8_3:56724304-56724323 Msa1296900:intron 15.0%
!! AAAAAAAAAAAGATTATGTG+GGG - chr8_3:56724723-56724742 Msa1296900:intron 15.0%
!!! AGAATGAATATAACAATTTT+TGG - chr8_3:56724936-56724955 Msa1296900:intron 15.0%
!! TAGGAAAGATTGAATAAAAT+TGG + chr8_3:56721996-56722015 None:intergenic 20.0%
!!! TTAATCTCAGATTTTTTCTT+TGG - chr8_3:56722073-56722092 Msa1296900:intron 20.0%
!! AGATAAGGAAAATAAATCTT+AGG + chr8_3:56722410-56722429 None:intergenic 20.0%
!! AAGGCAAATAAATAAATCTT+AGG + chr8_3:56722578-56722597 None:intergenic 20.0%
!! AAGATTTATTTATTTGCCTT+TGG - chr8_3:56722578-56722597 Msa1296900:intron 20.0%
!! AAACCTTAACATAGTAATTA+TGG + chr8_3:56722618-56722637 None:intergenic 20.0%
!!! TTACTATGTTAAGGTTTTAT+TGG - chr8_3:56722621-56722640 Msa1296900:intron 20.0%
!!! TTTTATATACATGATCATCA+TGG - chr8_3:56722647-56722666 Msa1296900:intron 20.0%
!! AATAACAGACAAAACTTTAT+CGG + chr8_3:56723077-56723096 None:intergenic 20.0%
!! ATCCTTTCAATATAAGATAT+GGG - chr8_3:56723187-56723206 Msa1296900:intron 20.0%
!! TTAATAGTGTACAGTAATTA+CGG + chr8_3:56723242-56723261 None:intergenic 20.0%
!!! TTATTTTCTAGATTGATACA+TGG + chr8_3:56723265-56723284 None:intergenic 20.0%
!! ACATAGAAAGTAAATAACTA+CGG + chr8_3:56723459-56723478 None:intergenic 20.0%
!!! ATAATTTGCTTGCTATTTTA+TGG - chr8_3:56723706-56723725 Msa1296900:intron 20.0%
!! AATTTCCCTTAATATGATAT+CGG - chr8_3:56724151-56724170 Msa1296900:intron 20.0%
!! TCTAATGAATTTAGAAAGAA+AGG + chr8_3:56724300-56724319 None:intergenic 20.0%
!! AATCAATAAAATATTCGAGT+TGG + chr8_3:56724360-56724379 None:intergenic 20.0%
!!! TTTTTACGTTCATATTCAAT+GGG - chr8_3:56724615-56724634 Msa1296900:intron 20.0%
!!! ACAATTTTTGGTTTAAAAAG+AGG - chr8_3:56724948-56724967 Msa1296900:intron 20.0%
!! ATGACTCAAATTTTCTGATA+TGG - chr8_3:56720703-56720722 Msa1296900:three_prime_UTR 25.0%
!!! TCTTCTTTTAGGCTTTAATT+TGG - chr8_3:56720932-56720951 Msa1296900:three_prime_UTR 25.0%
!! AATTTAGTGTTTGCTCTAAA+TGG - chr8_3:56720981-56721000 Msa1296900:three_prime_UTR 25.0%
!! ATTTAGTGTTTGCTCTAAAT+GGG - chr8_3:56720982-56721001 Msa1296900:three_prime_UTR 25.0%
! AAAAACAGGAAAATAAATGC+AGG + chr8_3:56721100-56721119 None:intergenic 25.0%
!!! GTTTTTTTTATCGTTTTTGC+TGG - chr8_3:56721367-56721386 Msa1296900:intron 25.0%
! TGGGTTAATATTAAACATGT+TGG - chr8_3:56721423-56721442 Msa1296900:intron 25.0%
! TCATGTTATATAGTGTTGTA+GGG - chr8_3:56721534-56721553 Msa1296900:intron 25.0%
! GACACTTATAATTCTACATT+AGG - chr8_3:56721556-56721575 Msa1296900:intron 25.0%
! TTGTAGAATCTCATCAAAAT+GGG + chr8_3:56721742-56721761 None:intergenic 25.0%
! ATAATTCTTAAAATGGGGTT+TGG - chr8_3:56721921-56721940 Msa1296900:intron 25.0%
! ATATTGAGACCATACTTTAT+TGG - chr8_3:56722535-56722554 Msa1296900:intron 25.0%
! ACATAGTAATTATGGACATT+TGG + chr8_3:56722610-56722629 None:intergenic 25.0%
! TGTCCATAATTACTATGTTA+AGG - chr8_3:56722612-56722631 Msa1296900:intron 25.0%
!! TCACTCTTTTACAGTATAAA+GGG + chr8_3:56722751-56722770 None:intergenic 25.0%
!! TTCACTCTTTTACAGTATAA+AGG + chr8_3:56722752-56722771 None:intergenic 25.0%
! ATTTCTCTTCATTGTTCATT+AGG + chr8_3:56722912-56722931 None:intergenic 25.0%
!! TACACCTAATTTTTATTCCT+GGG - chr8_3:56722971-56722990 Msa1296900:intron 25.0%
! TCACTTCATAGTAAAATTGA+AGG - chr8_3:56723161-56723180 Msa1296900:intron 25.0%
! GATCCTTTCAATATAAGATA+TGG - chr8_3:56723186-56723205 Msa1296900:intron 25.0%
! AATAACTTCAGAAAGAGAAA+AGG + chr8_3:56723304-56723323 None:intergenic 25.0%
!!! TTTGTTTCTAGCTTTATAGT+TGG - chr8_3:56723495-56723514 Msa1296900:intron 25.0%
! AATTATGGTAACTGAATTGA+AGG + chr8_3:56723747-56723766 None:intergenic 25.0%
! GTATTATAATACCTCCAAAT+TGG - chr8_3:56724074-56724093 Msa1296900:intron 25.0%
!! TGATTGTTCAAAGCTAATTT+TGG + chr8_3:56724197-56724216 None:intergenic 25.0%
!!! ATAAAATATTCGAGTTGGTA+CGG + chr8_3:56724355-56724374 None:intergenic 25.0%
!! AGAAGACAAGAATTTTCATA+TGG + chr8_3:56724399-56724418 None:intergenic 25.0%
! TGAAAATTCTTGTCTTCTTT+AGG - chr8_3:56724401-56724420 Msa1296900:intron 25.0%
!! GTTTTTACGTTCATATTCAA+TGG - chr8_3:56724614-56724633 Msa1296900:intron 25.0%
! TAATTGATGTATCTGTTAGA+CGG + chr8_3:56724778-56724797 None:intergenic 25.0%
!!! GCAATAGAAGATGTTTTTTA+CGG + chr8_3:56724817-56724836 None:intergenic 25.0%
! TTCAATTAGATAGAGTGCTT+TGG - chr8_3:56720758-56720777 Msa1296900:three_prime_UTR 30.0%
ATGTAACTGAAACCTTGATT+CGG - chr8_3:56720827-56720846 Msa1296900:three_prime_UTR 30.0%
! ATGCTTCAAAGGCTTTAATT+TGG - chr8_3:56720872-56720891 Msa1296900:three_prime_UTR 30.0%
! TGCTTCAAAGGCTTTAATTT+GGG - chr8_3:56720873-56720892 Msa1296900:three_prime_UTR 30.0%
!! CAAGTTGAGTTTCTTCTTTT+AGG - chr8_3:56720921-56720940 Msa1296900:three_prime_UTR 30.0%
!! AGGCTTTAATTTGGTTGTAT+AGG - chr8_3:56720941-56720960 Msa1296900:three_prime_UTR 30.0%
GAATTGTTGTCTATGAACTT+GGG - chr8_3:56721004-56721023 Msa1296900:three_prime_UTR 30.0%
TCTGCAATCACATAAAAAAC+AGG + chr8_3:56721114-56721133 None:intergenic 30.0%
TGAGAGAAATGAAACAATGA+TGG - chr8_3:56721158-56721177 Msa1296900:intron 30.0%
GAGAGAAATGAAACAATGAT+GGG - chr8_3:56721159-56721178 Msa1296900:intron 30.0%
TTAAACATGTTGGCATTTGT+TGG - chr8_3:56721433-56721452 Msa1296900:intron 30.0%
TTTGCTTATTCCTCTTTGAT+AGG - chr8_3:56721467-56721486 Msa1296900:intron 30.0%
GTCATGTTATATAGTGTTGT+AGG - chr8_3:56721533-56721552 Msa1296900:intron 30.0%
TCTTTGCAAAAATTTGAGTC+TGG + chr8_3:56721591-56721610 None:intergenic 30.0%
TGCAAAGATTGAAAATGTTG+AGG - chr8_3:56721603-56721622 Msa1296900:intron 30.0%
TGCATTTCTTCATTGTGTAT+TGG - chr8_3:56721631-56721650 Msa1296900:intron 30.0%
CTTGTAGAATCTCATCAAAA+TGG + chr8_3:56721743-56721762 None:intergenic 30.0%
! ACAAAGAATCTAACCTTTTC+TGG + chr8_3:56721816-56721835 None:intergenic 30.0%
!! ATTCTGTTTTGTTGTGCTTT+AGG - chr8_3:56721877-56721896 Msa1296900:intron 30.0%
TACCATCATATGATAACTTG+AGG - chr8_3:56722169-56722188 Msa1296900:intron 30.0%
GTTCTTATTGTATGACAATG+AGG - chr8_3:56722235-56722254 Msa1296900:intron 30.0%
TAACATTATGTGATGACTTG+AGG - chr8_3:56722265-56722284 Msa1296900:CDS 30.0%
! ATATTGAGACCATACTCTTT+TGG - chr8_3:56722368-56722387 Msa1296900:intron 30.0%
TGATATGTACCAAAAGAGTA+TGG + chr8_3:56722380-56722399 None:intergenic 30.0%
TCATATGATGGTAACAGATA+AGG + chr8_3:56722425-56722444 None:intergenic 30.0%
AGTCTCAAGTTATCATATGA+TGG + chr8_3:56722437-56722456 None:intergenic 30.0%
GCAAACTAATATGATAAGTG+AGG + chr8_3:56722469-56722488 None:intergenic 30.0%
TGATATGTGCCAATAAAGTA+TGG + chr8_3:56722547-56722566 None:intergenic 30.0%
! GTACACCTAATTTTTATTCC+TGG - chr8_3:56722970-56722989 Msa1296900:intron 30.0%
CAATCCCAGGAATAAAAATT+AGG + chr8_3:56722978-56722997 None:intergenic 30.0%
CACCCATATCTTATATTGAA+AGG + chr8_3:56723192-56723211 None:intergenic 30.0%
CTTTCAATATAAGATATGGG+TGG - chr8_3:56723190-56723209 Msa1296900:intron 30.0%
TTTAAGAACCTAGACAGAAA+TGG + chr8_3:56723218-56723237 None:intergenic 30.0%
!!! ATTTTTTGCAGAATATGCAG+AGG - chr8_3:56723346-56723365 Msa1296900:intron 30.0%
TGCAGTACATTCAATAATGA+CGG - chr8_3:56723387-56723406 Msa1296900:intron 30.0%
ATTAGATATTTGAGCCTTGA+GGG - chr8_3:56723631-56723650 Msa1296900:intron 30.0%
! GTTGTTTCAGAGTCAAATTA+TGG + chr8_3:56723762-56723781 None:intergenic 30.0%
! AATGATAGTAACTTGCATAG+TGG - chr8_3:56723885-56723904 Msa1296900:CDS 30.0%
! ATAGTGGAGAAAATGAAACA+GGG - chr8_3:56723901-56723920 Msa1296900:CDS 30.0%
TCTTTCAATCACTACCAATT+TGG + chr8_3:56724091-56724110 None:intergenic 30.0%
GTTGTCCGATATCATATTAA+GGG + chr8_3:56724159-56724178 None:intergenic 30.0%
AGTTGTCCGATATCATATTA+AGG + chr8_3:56724160-56724179 None:intergenic 30.0%
!! TAAAATATTCGAGTTGGTAC+GGG + chr8_3:56724354-56724373 None:intergenic 30.0%
TCGATTGATTGCTAAATATC+CGG - chr8_3:56724468-56724487 Msa1296900:intron 30.0%
TAAATATCCGGTTGTATTGT+AGG - chr8_3:56724480-56724499 Msa1296900:intron 30.0%
AAATATCCGGTTGTATTGTA+GGG - chr8_3:56724481-56724500 Msa1296900:intron 30.0%
! ATTCTTTTGTCTTCAGAACT+GGG + chr8_3:56724567-56724586 None:intergenic 30.0%
AGAAATAAGAGAAAGCTCTT+GGG + chr8_3:56724644-56724663 None:intergenic 30.0%
GTGAGAAAATTTGACAAAAG+AGG + chr8_3:56724687-56724706 None:intergenic 30.0%
TTCTATTGCAGTTTCTAAAC+CGG - chr8_3:56724828-56724847 Msa1296900:intron 30.0%
AATAACAACCTTACGAATGT+TGG - chr8_3:56724889-56724908 Msa1296900:intron 30.0%
ATATGCCAATCTCTGATGAA+AGG - chr8_3:56720735-56720754 Msa1296900:three_prime_UTR 35.0%
TTGAACCTTTCATCAGAGAT+TGG + chr8_3:56720743-56720762 None:intergenic 35.0%
! AATTAGATAGAGTGCTTTGG+TGG - chr8_3:56720761-56720780 Msa1296900:three_prime_UTR 35.0%
! ATTAGATAGAGTGCTTTGGT+GGG - chr8_3:56720762-56720781 Msa1296900:three_prime_UTR 35.0%
TACTTGCAGAATGTTCATCA+AGG - chr8_3:56720802-56720821 Msa1296900:three_prime_UTR 35.0%
GGAATTGTTGTCTATGAACT+TGG - chr8_3:56721003-56721022 Msa1296900:three_prime_UTR 35.0%
TGTGATTGCAGATGCTAAAA+AGG - chr8_3:56721122-56721141 Msa1296900:CDS 35.0%
GGATGAACTAATGAATGAAG+AGG - chr8_3:56721180-56721199 Msa1296900:intron 35.0%
TGAACTAATGAATGAAGAGG+AGG - chr8_3:56721183-56721202 Msa1296900:intron 35.0%
! GTCCTTTTTCTTCATTTTGC+TGG - chr8_3:56721295-56721314 Msa1296900:intron 35.0%
GACCAGCAAAATGAAGAAAA+AGG + chr8_3:56721300-56721319 None:intergenic 35.0%
! TTTTCTTCATTTTGCTGGTC+TGG - chr8_3:56721300-56721319 Msa1296900:intron 35.0%
!!! TTTTTTTTCCTGCTGTGTCT+AGG - chr8_3:56721391-56721410 Msa1296900:intron 35.0%
TTATTCCTCTTTGATAGGAG+TGG - chr8_3:56721472-56721491 Msa1296900:intron 35.0%
TTGAATCAGCATAGCTGATA+AGG + chr8_3:56721685-56721704 None:intergenic 35.0%
! AAGAATCTAACCTTTTCTGG+TGG + chr8_3:56721813-56721832 None:intergenic 35.0%
GTTCTTATTGTCTGACAATG+AGG - chr8_3:56722139-56722158 Msa1296900:intron 35.0%
AGCCTCAAGTTATCATATGA+TGG + chr8_3:56722174-56722193 None:intergenic 35.0%
TTGAGGCTCTATTGAATATC+TGG - chr8_3:56722186-56722205 Msa1296900:intron 35.0%
TCATGGTTAGCATTCAATGA+AGG - chr8_3:56722664-56722683 Msa1296900:intron 35.0%
GATTGTAGTTCAATCAGTCA+TGG - chr8_3:56722993-56723012 Msa1296900:intron 35.0%
ACAAAACTTTATCGGATCCT+AGG + chr8_3:56723069-56723088 None:intergenic 35.0%
ATTAGAGACAAGTTTCCTCA+CGG + chr8_3:56723131-56723150 None:intergenic 35.0%
TTGAATGTACTGCAGAGTAT+GGG + chr8_3:56723381-56723400 None:intergenic 35.0%
ATTGAATGTACTGCAGAGTA+TGG + chr8_3:56723382-56723401 None:intergenic 35.0%
AAACCCTTCATACTTATCGA+TGG - chr8_3:56723535-56723554 Msa1296900:intron 35.0%
GCTCAAATATCTAATCCAAG+TGG + chr8_3:56723626-56723645 None:intergenic 35.0%
GATTAGATATTTGAGCCTTG+AGG - chr8_3:56723630-56723649 Msa1296900:intron 35.0%
AATTGACAGTTTAACCCTCA+AGG + chr8_3:56723648-56723667 None:intergenic 35.0%
! AATTGAAGGCACATATCAAC+AGG + chr8_3:56723733-56723752 None:intergenic 35.0%
! AACTTTTACACATCGTCTAC+AGG - chr8_3:56723779-56723798 Msa1296900:intron 35.0%
!! GTCTACAGGTTTGTTATGAT+TGG - chr8_3:56723793-56723812 Msa1296900:intron 35.0%
! CATAGTGGAGAAAATGAAAC+AGG - chr8_3:56723900-56723919 Msa1296900:CDS 35.0%
GCATACTGCATAGTTTGATT+TGG - chr8_3:56724032-56724051 Msa1296900:intron 35.0%
TTCAATCACTACCAATTTGG+AGG + chr8_3:56724088-56724107 None:intergenic 35.0%
TTAAGGGAAATTACTACTGC+AGG + chr8_3:56724143-56724162 None:intergenic 35.0%
! ACGAAAAGGATAAGTGGATT+TGG + chr8_3:56724252-56724271 None:intergenic 35.0%
AAGAAAAGGATGACACGAAA+AGG + chr8_3:56724266-56724285 None:intergenic 35.0%
! CATCCTTTTCTTTACCTTAC+TGG - chr8_3:56724274-56724293 Msa1296900:intron 35.0%
TTTAGAAAGAAAGGCCAGTA+AGG + chr8_3:56724291-56724310 None:intergenic 35.0%
AATATCCGGTTGTATTGTAG+GGG - chr8_3:56724482-56724501 Msa1296900:intron 35.0%
TGCTAAAGATACCAGAAATC+CGG - chr8_3:56724524-56724543 Msa1296900:intron 35.0%
! CATTCTTTTGTCTTCAGAAC+TGG + chr8_3:56724568-56724587 None:intergenic 35.0%
CAGAAATAAGAGAAAGCTCT+TGG + chr8_3:56724645-56724664 None:intergenic 35.0%
GAGTAGGATGATTGTTAAAG+AGG - chr8_3:56724751-56724770 Msa1296900:intron 35.0%
TCTACTTACCAACATTCGTA+AGG + chr8_3:56724900-56724919 None:intergenic 35.0%
TGCTTTCAGAAACCGAATCA+AGG + chr8_3:56720842-56720861 None:intergenic 40.0%
!! TGATTCGGTTTCTGAAAGCA+AGG - chr8_3:56720842-56720861 Msa1296900:three_prime_UTR 40.0%
! GATTCGGTTTCTGAAAGCAA+GGG - chr8_3:56720843-56720862 Msa1296900:three_prime_UTR 40.0%
AAGGGAATGGAATGCTTCAA+AGG - chr8_3:56720861-56720880 Msa1296900:three_prime_UTR 40.0%
GCAGATGCTAAAAAGGATGA+AGG - chr8_3:56721129-56721148 Msa1296900:CDS 40.0%
AGTAGAAGAACAGCAAGAAG+AGG - chr8_3:56721210-56721229 Msa1296900:intron 40.0%
!!! CATTTTGCTGGTCTGGTTTT+TGG - chr8_3:56721307-56721326 Msa1296900:intron 40.0%
!! GTGGTTACTAGCTTTCTTTG+TGG - chr8_3:56721331-56721350 Msa1296900:intron 40.0%
GAGATTCTACAAGAAGCTGA+TGG - chr8_3:56721750-56721769 Msa1296900:CDS 40.0%
ATGGAATCATCTTGTCTCGT+GGG - chr8_3:56721769-56721788 Msa1296900:CDS 40.0%
ATCTTGTCTCGTGGGAATTT+GGG - chr8_3:56721777-56721796 Msa1296900:CDS 40.0%
CATTGATCTTCCACCAGAAA+AGG - chr8_3:56721800-56721819 Msa1296900:CDS 40.0%
GGTCTCAATATTTGCTTGCA+AGG + chr8_3:56722359-56722378 None:intergenic 40.0%
GGACATTTGGTTACTTCCAA+AGG + chr8_3:56722597-56722616 None:intergenic 40.0%
GTGCATTCATAAACTGTGTG+TGG + chr8_3:56722783-56722802 None:intergenic 40.0%
TTAATCCCGGTGAAGAATAC+AGG - chr8_3:56722811-56722830 Msa1296900:intron 40.0%
ACTGATTGAACTACAATCCC+AGG + chr8_3:56722991-56723010 None:intergenic 40.0%
AACTTTATCGGATCCTAGGT+CGG + chr8_3:56723065-56723084 None:intergenic 40.0%
! TTGCCCGTATTTTTCACTGT+GGG - chr8_3:56723104-56723123 Msa1296900:intron 40.0%
CGGTTCCATATATGCTTACA+CGG - chr8_3:56723407-56723426 Msa1296900:intron 40.0%
GCATACCGTGTAAGCATATA+TGG + chr8_3:56723415-56723434 None:intergenic 40.0%
GGTCCATCGATAAGTATGAA+GGG + chr8_3:56723541-56723560 None:intergenic 40.0%
AGGTCCATCGATAAGTATGA+AGG + chr8_3:56723542-56723561 None:intergenic 40.0%
! CATGATGTTTCTGCGAAATG+AGG + chr8_3:56723562-56723581 None:intergenic 40.0%
TATCTAATCCAAGTGGCAGA+TGG + chr8_3:56723619-56723638 None:intergenic 40.0%
AGTAATCCTCAAGAGCTTCA+TGG - chr8_3:56723930-56723949 Msa1296900:intron 40.0%
GTAATCCTCAAGAGCTTCAT+GGG - chr8_3:56723931-56723950 Msa1296900:intron 40.0%
CAAACTATGCAGTATGCTTG+AGG + chr8_3:56724029-56724048 None:intergenic 40.0%
!! TACTGCAGGCGATATTTTTG+GGG + chr8_3:56724129-56724148 None:intergenic 40.0%
! CTACTGCAGGCGATATTTTT+GGG + chr8_3:56724130-56724149 None:intergenic 40.0%
! ACTACTGCAGGCGATATTTT+TGG + chr8_3:56724131-56724150 None:intergenic 40.0%
GATGACACGAAAAGGATAAG+TGG + chr8_3:56724258-56724277 None:intergenic 40.0%
AGGCCAGTAAGGTAAAGAAA+AGG + chr8_3:56724280-56724299 None:intergenic 40.0%
TAAAGATACCAGAAATCCGG+CGG - chr8_3:56724527-56724546 Msa1296900:intron 40.0%
AGAAGATGCTTGACCTGATA+AGG + chr8_3:56724857-56724876 None:intergenic 40.0%
GGTGGGAGCTAATTAACTGA+TGG - chr8_3:56720779-56720798 Msa1296900:three_prime_UTR 45.0%
! GGTTTCTGAAAGCAAGGGAA+TGG - chr8_3:56720848-56720867 Msa1296900:three_prime_UTR 45.0%
!! AGCCTGTGTTGCTGAGATTT+TGG - chr8_3:56721271-56721290 Msa1296900:intron 45.0%
CACCAAAATCTCAGCAACAC+AGG + chr8_3:56721276-56721295 None:intergenic 45.0%
GATGGAATCATCTTGTCTCG+TGG - chr8_3:56721768-56721787 Msa1296900:CDS 45.0%
CATCTTGTCTCGTGGGAATT+TGG - chr8_3:56721776-56721795 Msa1296900:CDS 45.0%
TGCACTGTGACTGTTAATCC+CGG - chr8_3:56722798-56722817 Msa1296900:intron 45.0%
TGAGCTCCTGTATTCTTCAC+CGG + chr8_3:56722820-56722839 None:intergenic 45.0%
ACTTTATCGGATCCTAGGTC+GGG + chr8_3:56723064-56723083 None:intergenic 45.0%
CAGTGAAAAATACGGGCAAG+AGG + chr8_3:56723103-56723122 None:intergenic 45.0%
! CTTGCCCGTATTTTTCACTG+TGG - chr8_3:56723103-56723122 Msa1296900:intron 45.0%
! TGCCCGTATTTTTCACTGTG+GGG - chr8_3:56723105-56723124 Msa1296900:intron 45.0%
TGGAGCAACTACTGCAAAGA+TGG - chr8_3:56723813-56723832 Msa1296900:intron 45.0%
AGCAACTACTGCAAAGATGG+TGG - chr8_3:56723816-56723835 Msa1296900:intron 45.0%
!! ACTGCAAAGATGGTGGATCT+TGG - chr8_3:56723823-56723842 Msa1296900:intron 45.0%
ACCTAAATCCACTGCAAAGC+TGG - chr8_3:56723860-56723879 Msa1296900:CDS 45.0%
! ACCAGCTTTGCAGTGGATTT+AGG + chr8_3:56723864-56723883 None:intergenic 45.0%
ATCATTGACCAGCTTTGCAG+TGG + chr8_3:56723871-56723890 None:intergenic 45.0%
AACTTCCCATGAAGCTCTTG+AGG + chr8_3:56723939-56723958 None:intergenic 45.0%
TACACGATCAACAGGTCCAA+CGG + chr8_3:56723988-56724007 None:intergenic 45.0%
GGAGTAGATACACGATCAAC+AGG + chr8_3:56723996-56724015 None:intergenic 45.0%
GCAGTATGCTTGAGGATTAC+CGG + chr8_3:56724021-56724040 None:intergenic 45.0%
TTGGTACGGGTAAGGTCTAA+AGG + chr8_3:56724341-56724360 None:intergenic 45.0%
!! TATTCGAGTTGGTACGGGTA+AGG + chr8_3:56724349-56724368 None:intergenic 45.0%
ATGTGCCCCTACAATACAAC+CGG + chr8_3:56724490-56724509 None:intergenic 45.0%
! TGTATTGTAGGGGCACATCA+AGG - chr8_3:56724492-56724511 Msa1296900:intron 45.0%
ATTATGTGGGGAGCAAGAGT+AGG - chr8_3:56724735-56724754 Msa1296900:intron 45.0%
TCTAAACCGGTCACCTTATC+AGG - chr8_3:56724841-56724860 Msa1296900:intron 45.0%
!!! TGCTGGTCTGGTTTTTGGTG+TGG - chr8_3:56721312-56721331 Msa1296900:intron 50.0%
TAGGAGTGGCGTGGTATCAT+AGG - chr8_3:56721486-56721505 Msa1296900:intron 50.0%
GAGCTCCTGTATTCTTCACC+GGG + chr8_3:56722819-56722838 None:intergenic 50.0%
GGCCCCACAGTGAAAAATAC+GGG + chr8_3:56723110-56723129 None:intergenic 50.0%
CGGCCCCACAGTGAAAAATA+CGG + chr8_3:56723111-56723130 None:intergenic 50.0%
GGGTGGTTCCATTTCTGTCT+AGG - chr8_3:56723207-56723226 Msa1296900:intron 50.0%
GCTTAGATCCATCTGCCACT+TGG - chr8_3:56723608-56723627 Msa1296900:intron 50.0%
GATCGTGTATCTACTCCAGC+CGG - chr8_3:56723999-56724018 Msa1296900:intron 50.0%
TATGCTTGAGGATTACCGGC+TGG + chr8_3:56724017-56724036 None:intergenic 50.0%
GCTTGACCTGATAAGGTGAC+CGG + chr8_3:56724850-56724869 None:intergenic 50.0%
TTCCTGCTGTGTCTAGGTGC+AGG - chr8_3:56721397-56721416 Msa1296900:intron 55.0%
TCCTGCTGTGTCTAGGTGCA+GGG - chr8_3:56721398-56721417 Msa1296900:intron 55.0%
TGTGTCTAGGTGCAGGGTCT+GGG - chr8_3:56721404-56721423 Msa1296900:intron 55.0%
CCACGCCACTCCTATCAAAG+AGG + chr8_3:56721480-56721499 None:intergenic 55.0%
CCTCTTTGATAGGAGTGGCG+TGG - chr8_3:56721477-56721496 Msa1296900:intron 55.0%
! TTTTTCACTGTGGGGCCGTG+AGG - chr8_3:56723113-56723132 Msa1296900:intron 55.0%
GATCAACAGGTCCAACGGCA+CGG + chr8_3:56723983-56724002 None:intergenic 55.0%
TGCTTGAACCGCCGGATTTC+TGG + chr8_3:56724538-56724557 None:intergenic 55.0%
ACCCTGCACCTAGACACAGC+AGG + chr8_3:56721402-56721421 None:intergenic 60.0%
CTGTGTCTAGGTGCAGGGTC+TGG - chr8_3:56721403-56721422 Msa1296900:intron 60.0%
ATCCTAGGTCGGGACTGACG+GGG + chr8_3:56723054-56723073 None:intergenic 60.0%
GATCCTAGGTCGGGACTGAC+GGG + chr8_3:56723055-56723074 None:intergenic 60.0%
GGATCCTAGGTCGGGACTGA+CGG + chr8_3:56723056-56723075 None:intergenic 60.0%
! GGATCTGCTGCTTGAACCGC+CGG + chr8_3:56724546-56724565 None:intergenic 60.0%
GTCATGGCACCGCCCTGTCA+TGG - chr8_3:56723009-56723028 Msa1296900:intron 65.0%
ATGGCACCGCCCTGTCATGG+CGG - chr8_3:56723012-56723031 Msa1296900:intron 65.0%
CAGCTTCCGCCATGACAGGG+CGG + chr8_3:56723021-56723040 None:intergenic 65.0%
CCGCAGCTTCCGCCATGACA+GGG + chr8_3:56723024-56723043 None:intergenic 65.0%
! CCCTGTCATGGCGGAAGCTG+CGG - chr8_3:56723021-56723040 Msa1296900:intron 65.0%
! CCTGTCATGGCGGAAGCTGC+GGG - chr8_3:56723022-56723041 Msa1296900:intron 65.0%
! CGCAGTTCTAGCCGTGCCGT+TGG - chr8_3:56723969-56723988 Msa1296900:intron 65.0%
CCCGCAGCTTCCGCCATGAC+AGG + chr8_3:56723025-56723044 None:intergenic 70.0%
AGCCCCGTCAGTCCCGACCT+AGG - chr8_3:56723049-56723068 Msa1296900:intron 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 56720693 56724975 56720693 ID=Msa1296900;Name=Msa1296900
chr8_3 mRNA 56720693 56724975 56720693 ID=Msa1296900-mRNA-1;Parent=Msa1296900;Name=Msa1296900-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0.2|0.16|0.33|1|1|6|382|188
chr8_3 exon 56720693 56721166 56720693 ID=Msa1296900-mRNA-1:exon:16278;Parent=Msa1296900-mRNA-1
chr8_3 exon 56721648 56721867 56721648 ID=Msa1296900-mRNA-1:exon:16277;Parent=Msa1296900-mRNA-1
chr8_3 exon 56722240 56722311 56722240 ID=Msa1296900-mRNA-1:exon:16276;Parent=Msa1296900-mRNA-1
chr8_3 exon 56723847 56723936 56723847 ID=Msa1296900-mRNA-1:exon:16275;Parent=Msa1296900-mRNA-1
chr8_3 exon 56724170 56724255 56724170 ID=Msa1296900-mRNA-1:exon:16274;Parent=Msa1296900-mRNA-1
chr8_3 exon 56724969 56724975 56724969 ID=Msa1296900-mRNA-1:exon:16273;Parent=Msa1296900-mRNA-1
chr8_3 CDS 56724969 56724975 56724969 ID=Msa1296900-mRNA-1:cds;Parent=Msa1296900-mRNA-1
chr8_3 CDS 56724170 56724255 56724170 ID=Msa1296900-mRNA-1:cds;Parent=Msa1296900-mRNA-1
chr8_3 CDS 56723847 56723936 56723847 ID=Msa1296900-mRNA-1:cds;Parent=Msa1296900-mRNA-1
chr8_3 CDS 56722240 56722311 56722240 ID=Msa1296900-mRNA-1:cds;Parent=Msa1296900-mRNA-1
chr8_3 CDS 56721648 56721867 56721648 ID=Msa1296900-mRNA-1:cds;Parent=Msa1296900-mRNA-1
chr8_3 CDS 56721075 56721166 56721075 ID=Msa1296900-mRNA-1:cds;Parent=Msa1296900-mRNA-1
chr8_3 three_prime_UTR 56720693 56721074 56720693 ID=Msa1296900-mRNA-1:three_prime_utr;Parent=Msa1296900-mRNA-1
Gene Sequence

>Msa1296900

ATGGCAGGTGCAGGGTCTGGGTTAATATTAAACATGTTGGCATTTGTTGGTGAAGCAACATTTTGCTTATTCCTCTTTGATAGGAGTGGCGTGGGATTGACCCATTTTGATGAGATTCTACAAGAAGCTGATGGAATCATCTTGTCTCGTGGGAATTTGGGCATTGATCTTCCACCAGAAAAGAATATGCAGAGGTGTAGTCAGCCCATACTCTGCAGTACATTCAATAATGACGGTTCCATATATGCTTACACGGTTTGTTATGATTGGAGCAACTACTGCAAAGATGGTGGATCTTGGTCGACAACATTCTCACCTAAATCCACTGCAAAGCTGGTCAATGATAGTAACTTGCATAGTGGAGAAAATGAAACAGGGTTAGTGAGTAATCCTCAAGAGCTTCATGGGAAGTTGTCTGCTACTCGCAGTTCTAGCCGTGCCGTTGGACCTGTTGATCGTGTATCTACTCCAGCCGGGGCACATCAAGGAAGCATTTTTGCTAAAGATACCAGAAATCCGGCGGTTCAAGCAGCAGATCCCAGTTCTGAAGACAAAAGAATGCAGTGA
Protein sequence

>Msa1296900

MAGAGSGLILNMLAFVGEATFCLFLFDRSGVGLTHFDEILQEADGIILSRGNLGIDLPPEKNMQRCSQPILCSTFNNDGSIYAYTVCYDWSNYCKDGGSWSTTFSPKSTAKLVNDSNLHSGENETGLVSNPQELHGKLSATRSSSRAVGPVDRVSTPAGAHQGSIFAKDTRNPAVQAADPSSEDKRMQ*