AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1012240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1012240 MtrunA17_Chr3g0138401 88.542 96 8 1 25 117 321 416 5.68e-52 168
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTTGAACAGCCAGATTTC+TGG 0.202017 7_1:+17315957 None:intergenic
TCTAAACCGGTCACCTTATC+AGG 0.216944 7_1:-17315650 Msa1012240:three_prime_UTR
ACCAGCTTTGCAGTGGATTT+AGG 0.291769 7_1:+17316648 None:intergenic
TTGTGGTTATGTGATGAATT+TGG 0.323335 7_1:-17318355 None:intergenic
TGCTAAAGATACCAGAAATC+TGG 0.323635 7_1:-17315968 Msa1012240:CDS
GTCTACAGGTTTGTTATGAT+TGG 0.341574 7_1:-17316716 Msa1012240:intron
CATAGTGGAGAAAATGAAAC+AGG 0.343306 7_1:-17316609 Msa1012240:CDS
CAGAAATAAGAGAAAGCTCT+TGG 0.383667 7_1:+17315850 None:intergenic
TTCTATTGCAGTTTCTAAAC+CGG 0.384539 7_1:-17315663 Msa1012240:three_prime_UTR
CATGGGAAGTTGTCTGCTAC+TGG 0.416162 7_1:-17316561 Msa1012240:CDS
GCCTAAATCCACTGCAAAGC+TGG 0.430607 7_1:-17316649 Msa1012240:CDS
AGAAGATGCTTGACCTGATA+AGG 0.443032 7_1:+17315637 None:intergenic
GGAGTAGATACACGATCAAC+AGG 0.476638 7_1:+17316516 None:intergenic
ACTGCAAAGATGGTGGATCT+TGG 0.479375 7_1:-17316686 Msa1012240:CDS
ATGAATTTGGTGGTGGAAGG+TGG 0.483065 7_1:-17318342 Msa1012240:CDS
GTGAGAAAATTTGACAAAAG+AGG 0.485887 7_1:+17315808 None:intergenic
AGAAATAAGAGAAAGCTCTT+GGG 0.490918 7_1:+17315851 None:intergenic
AGTAATCCTCAAGAGCTTCA+TGG 0.497229 7_1:-17316579 Msa1012240:CDS
GAGTAGGATGATTGTTAAAG+AGG 0.509074 7_1:-17315740 Msa1012240:three_prime_UTR
TGGTTATGTGATGAATTTGG+TGG 0.511386 7_1:-17318352 None:intergenic
TGGAAGAATGTGGTGGTTGG+AGG 0.515352 7_1:-17318322 Msa1012240:intron
AGGTGGAAGAATGTGGTGGT+TGG 0.515519 7_1:-17318325 Msa1012240:CDS
AACTTCCCATGAAGCTCTTG+AGG 0.518605 7_1:+17316573 None:intergenic
ATTATGTGGGGAGCAAGAGT+AGG 0.524042 7_1:-17315756 Msa1012240:three_prime_UTR
GTAATCCTCAAGAGCTTCAT+GGG 0.525373 7_1:-17316578 Msa1012240:CDS
AATAACAACCTTACGAATGT+TGG 0.526846 7_1:-17315602 Msa1012240:three_prime_UTR
ATCATTGACCAGCTTTGCAG+TGG 0.539773 7_1:+17316641 None:intergenic
GGCAGTTCTAGCCGTGCCGT+TGG 0.541164 7_1:-17316540 Msa1012240:CDS
TTATGTGATGAATTTGGTGG+TGG 0.543720 7_1:-17318349 None:intergenic
TGGAGCAACTACTGCAAAGA+TGG 0.553115 7_1:-17316696 Msa1012240:CDS
TCTACTTACCAACATTCGTA+AGG 0.553647 7_1:+17315594 None:intergenic
GCTTGACCTGATAAGGTGAC+CGG 0.558454 7_1:+17315644 None:intergenic
TGGTGGAAGGTGGAAGAATG+TGG 0.562417 7_1:-17318332 Msa1012240:CDS
AATGATAGTAACTTGCATAG+TGG 0.579416 7_1:-17316624 Msa1012240:CDS
GTGATGAATTTGGTGGTGGA+AGG 0.583527 7_1:-17318345 Msa1012240:CDS
GATCGTGTATCTACTCCAGC+CGG 0.597972 7_1:-17316510 Msa1012240:intron
TACACGATCAACAGGTCCAA+CGG 0.603243 7_1:+17316524 None:intergenic
TAATTGATGTATCTGTTAGA+CGG 0.604298 7_1:+17315716 None:intergenic
GATCAACAGGTCCAACGGCA+CGG 0.605689 7_1:+17316529 None:intergenic
TGTATTGTAGGGGCACATCA+AGG 0.610537 7_1:-17316000 Msa1012240:intron
AGCAACTACTGCAAAGATGG+TGG 0.619072 7_1:-17316693 Msa1012240:CDS
TGGAAGGTGGAAGAATGTGG+TGG 0.656551 7_1:-17318329 Msa1012240:CDS
ATAGTGGAGAAAATGAAACA+GGG 0.722474 7_1:-17316608 Msa1012240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAGTTTAATTTTCATTT+CGG + chr7_1:17316006-17316025 None:intergenic 10.0%
!!! CATAATCTTTTTTTTTTTTA+AGG + chr7_1:17318107-17318126 None:intergenic 10.0%
!! TAAAAAAAAAAAAGATTATG+TGG - chr7_1:17318107-17318126 Msa1012240:intron 10.0%
!! AAAAAAAAAAAAGATTATGT+GGG - chr7_1:17318108-17318127 Msa1012240:intron 10.0%
!!! AAAATGATTTTGTGATTTTT+TGG - chr7_1:17315772-17315791 Msa1012240:three_prime_UTR 15.0%
!!! TTTTAAAATGGATTTTTAAC+AGG - chr7_1:17316170-17316189 Msa1012240:intron 15.0%
!!! TTTAAAATGGATTTTTAACA+GGG - chr7_1:17316171-17316190 Msa1012240:intron 15.0%
!! TTTGCAAAATTCATATAAAA+GGG + chr7_1:17316606-17316625 None:intergenic 15.0%
!! TTCTAAATTCATTAGAATAT+TGG - chr7_1:17317689-17317708 Msa1012240:intron 15.0%
!!! TTTTTATGTTCATATTCAAT+GGG - chr7_1:17318000-17318019 Msa1012240:intron 15.0%
!! AAAAAAAAAAAGATTATGTG+GGG - chr7_1:17318109-17318128 Msa1012240:intron 15.0%
!!! AGAATGAATATAACAATTTT+TGG - chr7_1:17318322-17318341 Msa1012240:intron 15.0%
!! CTTCAAATGCAAATTAAAAA+TGG - chr7_1:17316316-17316335 Msa1012240:intron 20.0%
!!! ATTGTTTGAGTCTTTTAAAT+TGG + chr7_1:17316539-17316558 None:intergenic 20.0%
!! GTTTGCAAAATTCATATAAA+AGG + chr7_1:17316607-17316626 None:intergenic 20.0%
!!! AGAATTGAACAAGTATTTTT+AGG - chr7_1:17316701-17316720 Msa1012240:CDS 20.0%
!!! TATTTTTAGGTCCAAAATTA+GGG - chr7_1:17316714-17316733 Msa1012240:intron 20.0%
!! ACATAGAAAGTAAATAACTA+CGG + chr7_1:17316827-17316846 None:intergenic 20.0%
!!! ATAATTTGCTTGCTATTTTA+TGG - chr7_1:17317074-17317093 Msa1012240:intron 20.0%
!! AATTTCCCTTAATATGATAT+CGG - chr7_1:17317519-17317538 Msa1012240:intron 20.0%
!! TCTAATGAATTTAGAAAGAA+AGG + chr7_1:17317685-17317704 None:intergenic 20.0%
!! AATCAATAAAATATTCGAGT+TGG + chr7_1:17317745-17317764 None:intergenic 20.0%
!!! GTTTTTATGTTCATATTCAA+TGG - chr7_1:17317999-17318018 Msa1012240:intron 20.0%
!!! ACAATTTTTGGTTTAAAAAG+AGG - chr7_1:17318334-17318353 Msa1012240:CDS 20.0%
! TGAAAAGTTAGTTAGAAAAG+AGG - chr7_1:17315726-17315745 Msa1012240:three_prime_UTR 25.0%
! GAAAAGTTAGTTAGAAAAGA+GGG - chr7_1:17315727-17315746 Msa1012240:three_prime_UTR 25.0%
!! TGCAAATAACAAAAGTACAA+AGG - chr7_1:17315980-17315999 Msa1012240:CDS 25.0%
!!! ACTCAGATGCAATTTTAAAA+TGG - chr7_1:17316158-17316177 Msa1012240:intron 25.0%
! ACTTATTCAAACTATTTGAG+AGG - chr7_1:17316364-17316383 Msa1012240:intron 25.0%
!!! TCAAACAAGTTCTAAAAGAT+TGG - chr7_1:17316389-17316408 Msa1012240:intron 25.0%
!! ATTGGAAGAGATTTTATCAT+GGG - chr7_1:17316407-17316426 Msa1012240:intron 25.0%
!! AAAGACTCAAACAATTTTCA+AGG - chr7_1:17316544-17316563 Msa1012240:CDS 25.0%
! AATGACTTATAATGCAAGAA+AGG - chr7_1:17316677-17316696 Msa1012240:CDS 25.0%
!!! GTATTTTTAGGTCCAAAATT+AGG - chr7_1:17316713-17316732 Msa1012240:intron 25.0%
!!! TTTGTTTCTAGCTTTATAGT+TGG - chr7_1:17316863-17316882 Msa1012240:intron 25.0%
! AATTATGGTAACTGAATTGA+AGG + chr7_1:17317115-17317134 None:intergenic 25.0%
! GTATTATAATACCTCCAAAT+TGG - chr7_1:17317442-17317461 Msa1012240:intron 25.0%
!! TGATTGTTCAAAGCTAATTT+TGG + chr7_1:17317565-17317584 None:intergenic 25.0%
!!! ATAAAATATTCGAGTTGGTA+CGG + chr7_1:17317740-17317759 None:intergenic 25.0%
!! AGAAGACAAGAATTTTCATA+TGG + chr7_1:17317784-17317803 None:intergenic 25.0%
! TGAAAATTCTTGTCTTCTTT+AGG - chr7_1:17317786-17317805 Msa1012240:intron 25.0%
! TAATTGATGTATCTGTTAGA+CGG + chr7_1:17318164-17318183 None:intergenic 25.0%
!!! GCAATAGAAGATGTTTTTTA+CGG + chr7_1:17318203-17318222 None:intergenic 25.0%
AAAAGAGGGAAAAAATTGAG+AGG - chr7_1:17315741-17315760 Msa1012240:three_prime_UTR 30.0%
! ACCAAGACTTTGACTTATAT+AGG - chr7_1:17315830-17315849 Msa1012240:three_prime_UTR 30.0%
ACCTATATAAGTCAAAGTCT+TGG + chr7_1:17315834-17315853 None:intergenic 30.0%
TGACTTATATAGGTGATGAA+AGG - chr7_1:17315840-17315859 Msa1012240:three_prime_UTR 30.0%
GACTTATATAGGTGATGAAA+GGG - chr7_1:17315841-17315860 Msa1012240:three_prime_UTR 30.0%
!! TGACTTGTAACAAACTTTTG+TGG - chr7_1:17315899-17315918 Msa1012240:three_prime_UTR 30.0%
GGACTCTAACAAACTAAAAA+AGG - chr7_1:17315950-17315969 Msa1012240:CDS 30.0%
! GATTGGAAGAGATTTTATCA+TGG - chr7_1:17316406-17316425 Msa1012240:intron 30.0%
TTTGACATGAGATTGAAACA+AGG - chr7_1:17316502-17316521 Msa1012240:intron 30.0%
! GATTAAAATGAGTTGAATGC+AGG - chr7_1:17316566-17316585 Msa1012240:CDS 30.0%
TCATTATTGAATGTACTGCA+GGG + chr7_1:17316755-17316774 None:intergenic 30.0%
TGCAGTACATTCAATAATGA+CGG - chr7_1:17316755-17316774 Msa1012240:intron 30.0%
ATTAGATATTTGAGCCTTGA+GGG - chr7_1:17316999-17317018 Msa1012240:intron 30.0%
! GTTGTTTCAGAGTCAAATTA+TGG + chr7_1:17317130-17317149 None:intergenic 30.0%
! AATGATAGTAACTTGCATAG+TGG - chr7_1:17317253-17317272 Msa1012240:intron 30.0%
! ATAGTGGAGAAAATGAAACA+GGG - chr7_1:17317269-17317288 Msa1012240:intron 30.0%
TCTTTCAATCACTACCAATT+TGG + chr7_1:17317459-17317478 None:intergenic 30.0%
GTTGTCCGATATCATATTAA+GGG + chr7_1:17317527-17317546 None:intergenic 30.0%
AGTTGTCCGATATCATATTA+AGG + chr7_1:17317528-17317547 None:intergenic 30.0%
ATCAGTTGAGATTGAAAACT+AGG - chr7_1:17317598-17317617 Msa1012240:intron 30.0%
!! TAAAATATTCGAGTTGGTAC+GGG + chr7_1:17317739-17317758 None:intergenic 30.0%
TCGATTGATTGCTAAATATC+CGG - chr7_1:17317853-17317872 Msa1012240:intron 30.0%
TAAATATCCGGTTGTATTGT+AGG - chr7_1:17317865-17317884 Msa1012240:intron 30.0%
AAATATCCGGTTGTATTGTA+GGG - chr7_1:17317866-17317885 Msa1012240:intron 30.0%
AGAAATAAGAGAAAGCTCTT+GGG + chr7_1:17318029-17318048 None:intergenic 30.0%
GTGAGAAAATTTGACAAAAG+AGG + chr7_1:17318072-17318091 None:intergenic 30.0%
TTCTATTGCAGTTTCTAAAC+CGG - chr7_1:17318214-17318233 Msa1012240:intron 30.0%
AATAACAACCTTACGAATGT+TGG - chr7_1:17318275-17318294 Msa1012240:intron 30.0%
!! AAGGTGGTTAGAGTTTGTTA+GGG - chr7_1:17315669-17315688 Msa1012240:three_prime_UTR 35.0%
AGAGGATGAGAAAGTTAGTT+AGG - chr7_1:17315699-17315718 Msa1012240:three_prime_UTR 35.0%
GAGGATGAGAAAGTTAGTTA+GGG - chr7_1:17315700-17315719 Msa1012240:three_prime_UTR 35.0%
ACCAATGAAATAATGCCATG+TGG - chr7_1:17315872-17315891 Msa1012240:three_prime_UTR 35.0%
! TTTTGTGGACCAACAACATT+TGG - chr7_1:17315914-17315933 Msa1012240:exon 35.0%
!! TTTAGTCGTTTTAGTCCCTA+GGG + chr7_1:17316134-17316153 None:intergenic 35.0%
!! TTTTAGTCGTTTTAGTCCCT+AGG + chr7_1:17316135-17316154 None:intergenic 35.0%
AAAAGACTAAAATGCCCCTA+GGG - chr7_1:17316273-17316292 Msa1012240:intron 35.0%
!!! GGCTTGTTATACCCTAATTT+TGG + chr7_1:17316728-17316747 None:intergenic 35.0%
GTCATTATTGAATGTACTGC+AGG + chr7_1:17316756-17316775 None:intergenic 35.0%
AAACCCTTCATACTTATCGA+TGG - chr7_1:17316903-17316922 Msa1012240:intron 35.0%
GCTCAAATATCTAATCCAAG+TGG + chr7_1:17316994-17317013 None:intergenic 35.0%
GATTAGATATTTGAGCCTTG+AGG - chr7_1:17316998-17317017 Msa1012240:intron 35.0%
AATTGACAGTTTAACCCTCA+AGG + chr7_1:17317016-17317035 None:intergenic 35.0%
! AATTGAAGGCACATATCAAC+AGG + chr7_1:17317101-17317120 None:intergenic 35.0%
! AACTTTTACACATCGTCTAC+AGG - chr7_1:17317147-17317166 Msa1012240:intron 35.0%
!! GTCTACAGGTTTGTTATGAT+TGG - chr7_1:17317161-17317180 Msa1012240:intron 35.0%
! CATAGTGGAGAAAATGAAAC+AGG - chr7_1:17317268-17317287 Msa1012240:intron 35.0%
GCATACTGCATAGTTTGATT+TGG - chr7_1:17317400-17317419 Msa1012240:intron 35.0%
TTCAATCACTACCAATTTGG+AGG + chr7_1:17317456-17317475 None:intergenic 35.0%
! TGGTAGTGATTGAAAGACTA+CGG - chr7_1:17317462-17317481 Msa1012240:intron 35.0%
TTAAGGGAAATTACTACTGC+AGG + chr7_1:17317511-17317530 None:intergenic 35.0%
AAGAAAAGGATGACACGAAA+AGG + chr7_1:17317651-17317670 None:intergenic 35.0%
! CATCCTTTTCTTTACCTTAC+TGG - chr7_1:17317659-17317678 Msa1012240:intron 35.0%
TTTAGAAAGAAAGGCCAGTA+AGG + chr7_1:17317676-17317695 None:intergenic 35.0%
AATATCCGGTTGTATTGTAG+GGG - chr7_1:17317867-17317886 Msa1012240:intron 35.0%
TGCTAAAGATACCAGAAATC+TGG - chr7_1:17317909-17317928 Msa1012240:intron 35.0%
! GTTCTTTTGTCTTCAGAACT+GGG + chr7_1:17317952-17317971 None:intergenic 35.0%
CAGAAATAAGAGAAAGCTCT+TGG + chr7_1:17318030-17318049 None:intergenic 35.0%
GAGTAGGATGATTGTTAAAG+AGG - chr7_1:17318137-17318156 Msa1012240:intron 35.0%
TCTACTTACCAACATTCGTA+AGG + chr7_1:17318286-17318305 None:intergenic 35.0%
ATGAAGAAAGTGGAGGTTGA+AGG - chr7_1:17315637-17315656 Msa1012240:three_prime_UTR 40.0%
! GAAGGTGGTTAGAGTTTGTT+AGG - chr7_1:17315668-17315687 Msa1012240:three_prime_UTR 40.0%
GATGAGAAAGTTAGTTAGGG+TGG - chr7_1:17315703-17315722 Msa1012240:three_prime_UTR 40.0%
GCCACATGGCATTATTTCAT+TGG + chr7_1:17315876-17315895 None:intergenic 40.0%
!! TTAGTCGTTTTAGTCCCTAG+GGG + chr7_1:17316133-17316152 None:intergenic 40.0%
GAAAAGACTAAAATGCCCCT+AGG - chr7_1:17316272-17316291 Msa1012240:intron 40.0%
AAAGACTAAAATGCCCCTAG+GGG - chr7_1:17316274-17316293 Msa1012240:intron 40.0%
AAATGACCAAAATGCCCCTA+GGG - chr7_1:17316430-17316449 Msa1012240:intron 40.0%
TTTAGTCAGTTTAGCCCCTA+GGG + chr7_1:17316448-17316467 None:intergenic 40.0%
! TTTTAGTCAGTTTAGCCCCT+AGG + chr7_1:17316449-17316468 None:intergenic 40.0%
TTGAATGTACTGCAGGGTAT+GGG + chr7_1:17316749-17316768 None:intergenic 40.0%
ATTGAATGTACTGCAGGGTA+TGG + chr7_1:17316750-17316769 None:intergenic 40.0%
GGTCCATCGATAAGTATGAA+GGG + chr7_1:17316909-17316928 None:intergenic 40.0%
AGGTCCATCGATAAGTATGA+AGG + chr7_1:17316910-17316929 None:intergenic 40.0%
! CATGATGTTTCTGCGAAATG+AGG + chr7_1:17316930-17316949 None:intergenic 40.0%
TATCTAATCCAAGTGGCAGA+TGG + chr7_1:17316987-17317006 None:intergenic 40.0%
AGTAATCCTCAAGAGCTTCA+TGG - chr7_1:17317298-17317317 Msa1012240:intron 40.0%
GTAATCCTCAAGAGCTTCAT+GGG - chr7_1:17317299-17317318 Msa1012240:intron 40.0%
CAAACTATGCAGTATGCTTG+AGG + chr7_1:17317397-17317416 None:intergenic 40.0%
!! TACTGCAGGCGATATTTTTG+GGG + chr7_1:17317497-17317516 None:intergenic 40.0%
! CTACTGCAGGCGATATTTTT+GGG + chr7_1:17317498-17317517 None:intergenic 40.0%
! ACTACTGCAGGCGATATTTT+TGG + chr7_1:17317499-17317518 None:intergenic 40.0%
! ACGAAAAGGATCAGTGGATT+TGG + chr7_1:17317637-17317656 None:intergenic 40.0%
AGGCCAGTAAGGTAAAGAAA+AGG + chr7_1:17317665-17317684 None:intergenic 40.0%
! CGTTCTTTTGTCTTCAGAAC+TGG + chr7_1:17317953-17317972 None:intergenic 40.0%
AGAAGATGCTTGACCTGATA+AGG + chr7_1:17318243-17318262 None:intergenic 40.0%
!! ATGAATTTGGTGGTGGAAGG+TGG - chr7_1:17315535-17315554 Msa1012240:three_prime_UTR 45.0%
! GGTTGTTAGGTGGTGGTTTA+TGG - chr7_1:17315576-17315595 Msa1012240:three_prime_UTR 45.0%
! TGTTAGGTGGTGGTTTATGG+TGG - chr7_1:17315579-17315598 Msa1012240:three_prime_UTR 45.0%
AGGTTTGTGGTGGTTGTTAG+AGG - chr7_1:17315608-17315627 Msa1012240:three_prime_UTR 45.0%
TTGTGGTGGTTGTTAGAGGT+TGG - chr7_1:17315612-17315631 Msa1012240:three_prime_UTR 45.0%
GAGGTTGGAGATGAAGAAAG+TGG - chr7_1:17315627-17315646 Msa1012240:three_prime_UTR 45.0%
GTTGGAGATGAAGAAAGTGG+AGG - chr7_1:17315630-17315649 Msa1012240:three_prime_UTR 45.0%
AAGAAAGTGGAGGTTGAAGG+TGG - chr7_1:17315640-17315659 Msa1012240:three_prime_UTR 45.0%
!! GTGGTTAGAGTTTGTTAGGG+TGG - chr7_1:17315672-17315691 Msa1012240:three_prime_UTR 45.0%
! GTTTGTTAGGGTGGAGATAG+AGG - chr7_1:17315681-17315700 Msa1012240:three_prime_UTR 45.0%
TAGGTGATGAAAGGGATGCT+TGG - chr7_1:17315849-17315868 Msa1012240:three_prime_UTR 45.0%
GTTACAAGTCAAGTGCCACA+TGG + chr7_1:17315890-17315909 None:intergenic 45.0%
ACCAACAACATTTGGACACG+TGG - chr7_1:17315922-17315941 Msa1012240:CDS 45.0%
AAATGACCAAAACGCCCCTA+GGG - chr7_1:17316116-17316135 Msa1012240:intron 45.0%
! TTGAGTCAGTTTTGCCCCTA+GGG + chr7_1:17316291-17316310 None:intergenic 45.0%
! TTTGAGTCAGTTTTGCCCCT+AGG + chr7_1:17316292-17316311 None:intergenic 45.0%
GAAATGACCAAAATGCCCCT+AGG - chr7_1:17316429-17316448 Msa1012240:intron 45.0%
AATGACCAAAATGCCCCTAG+GGG - chr7_1:17316431-17316450 Msa1012240:intron 45.0%
TTAGTCAGTTTAGCCCCTAG+GGG + chr7_1:17316447-17316466 None:intergenic 45.0%
CGGTTCCATATATGCTTACG+CGG - chr7_1:17316775-17316794 Msa1012240:intron 45.0%
GCATACCGCGTAAGCATATA+TGG + chr7_1:17316783-17316802 None:intergenic 45.0%
TGGAGCAACTACTGCAAAGA+TGG - chr7_1:17317181-17317200 Msa1012240:intron 45.0%
AGCAACTACTGCAAAGATGG+TGG - chr7_1:17317184-17317203 Msa1012240:intron 45.0%
!! ACTGCAAAGATGGTGGATCT+TGG - chr7_1:17317191-17317210 Msa1012240:intron 45.0%
! ACCAGCTTTGCAGTGGATTT+AGG + chr7_1:17317232-17317251 None:intergenic 45.0%
ATCATTGACCAGCTTTGCAG+TGG + chr7_1:17317239-17317258 None:intergenic 45.0%
AACTTCCCATGAAGCTCTTG+AGG + chr7_1:17317307-17317326 None:intergenic 45.0%
TACACGATCAACAGGTCCAA+CGG + chr7_1:17317356-17317375 None:intergenic 45.0%
GGAGTAGATACACGATCAAC+AGG + chr7_1:17317364-17317383 None:intergenic 45.0%
GCAGTATGCTTGAGGATTAC+CGG + chr7_1:17317389-17317408 None:intergenic 45.0%
GATGACACGAAAAGGATCAG+TGG + chr7_1:17317643-17317662 None:intergenic 45.0%
ATGTGCCCCTACAATACAAC+CGG + chr7_1:17317875-17317894 None:intergenic 45.0%
! TGTATTGTAGGGGCACATCA+AGG - chr7_1:17317877-17317896 Msa1012240:intron 45.0%
TGCTTGAACAGCCAGATTTC+TGG + chr7_1:17317923-17317942 None:intergenic 45.0%
ATTATGTGGGGAGCAAGAGT+AGG - chr7_1:17318121-17318140 Msa1012240:intron 45.0%
TCTAAACCGGTCACCTTATC+AGG - chr7_1:17318227-17318246 Msa1012240:intron 45.0%
TGGTGGAAGGTGGAAGAATG+TGG - chr7_1:17315545-17315564 Msa1012240:three_prime_UTR 50.0%
TGGAAGGTGGAAGAATGTGG+TGG - chr7_1:17315548-17315567 Msa1012240:three_prime_UTR 50.0%
AGGTGGAAGAATGTGGTGGT+TGG - chr7_1:17315552-17315571 Msa1012240:three_prime_UTR 50.0%
TGGAAGAATGTGGTGGTTGG+AGG - chr7_1:17315555-17315574 Msa1012240:three_prime_UTR 50.0%
TGTGGTGGTTGGAGGTTGTT+AGG - chr7_1:17315563-17315582 Msa1012240:three_prime_UTR 50.0%
GTGGTGGTTTATGGTGGTTG+TGG - chr7_1:17315585-17315604 Msa1012240:three_prime_UTR 50.0%
GTGGTTTATGGTGGTTGTGG+AGG - chr7_1:17315588-17315607 Msa1012240:three_prime_UTR 50.0%
ATGGTGGTTGTGGAGGTTTG+TGG - chr7_1:17315595-17315614 Msa1012240:three_prime_UTR 50.0%
AAAGTGGAGGTTGAAGGTGG+TGG - chr7_1:17315643-17315662 Msa1012240:three_prime_UTR 50.0%
! AGGTTGAAGGTGGTGGTTGA+AGG - chr7_1:17315650-17315669 Msa1012240:three_prime_UTR 50.0%
! TTGAAGGTGGTGGTTGAAGG+TGG - chr7_1:17315653-17315672 Msa1012240:three_prime_UTR 50.0%
GCCACGTGTCCAAATGTTGT+TGG + chr7_1:17315926-17315945 None:intergenic 50.0%
ACATTTGGACACGTGGCACT+TGG - chr7_1:17315929-17315948 Msa1012240:CDS 50.0%
GAAATGACCAAAACGCCCCT+AGG - chr7_1:17316115-17316134 Msa1012240:intron 50.0%
!!! TTTAGTCCCTAGGGGCGTTT+TGG + chr7_1:17316125-17316144 None:intergenic 50.0%
! TGAGTCAGTTTTGCCCCTAG+GGG + chr7_1:17316290-17316309 None:intergenic 50.0%
!!! TTTAGCCCCTAGGGGCATTT+TGG + chr7_1:17316439-17316458 None:intergenic 50.0%
GCTTAGATCCATCTGCCACT+TGG - chr7_1:17316976-17316995 Msa1012240:intron 50.0%
GCCTAAATCCACTGCAAAGC+TGG - chr7_1:17317228-17317247 Msa1012240:intron 50.0%
CATGGGAAGTTGTCTGCTAC+TGG - chr7_1:17317316-17317335 Msa1012240:intron 50.0%
GATCGTGTATCTACTCCAGC+CGG - chr7_1:17317367-17317386 Msa1012240:intron 50.0%
TATGCTTGAGGATTACCGGC+TGG + chr7_1:17317385-17317404 None:intergenic 50.0%
TTGGTACGGGCAAGGTCTAA+AGG + chr7_1:17317726-17317745 None:intergenic 50.0%
!! TATTCGAGTTGGTACGGGCA+AGG + chr7_1:17317734-17317753 None:intergenic 50.0%
GCTTGACCTGATAAGGTGAC+CGG + chr7_1:17318236-17318255 None:intergenic 50.0%
GGTGGTTGGAGGTTGTTAGG+TGG - chr7_1:17315566-17315585 Msa1012240:three_prime_UTR 55.0%
GGTTGGAGGTTGTTAGGTGG+TGG - chr7_1:17315569-17315588 Msa1012240:three_prime_UTR 55.0%
GTGGTTGTGGAGGTTTGTGG+TGG - chr7_1:17315598-17315617 Msa1012240:three_prime_UTR 55.0%
GATCAACAGGTCCAACGGCA+CGG + chr7_1:17317351-17317370 None:intergenic 55.0%
! GGCAGTTCTAGCCGTGCCGT+TGG - chr7_1:17317337-17317356 Msa1012240:intron 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 17315535 17318364 17315535 ID=Msa1012240;Name=Msa1012240
chr7_1 mRNA 17315535 17318364 17315535 ID=Msa1012240-mRNA-1;Parent=Msa1012240;Name=Msa1012240-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0.5|0.33|0.66|1|1|3|386|117
chr7_1 exon 17315535 17316012 17315535 ID=Msa1012240-mRNA-1:exon:4347;Parent=Msa1012240-mRNA-1
chr7_1 exon 17316511 17316730 17316511 ID=Msa1012240-mRNA-1:exon:4346;Parent=Msa1012240-mRNA-1
chr7_1 exon 17318323 17318364 17318323 ID=Msa1012240-mRNA-1:exon:4345;Parent=Msa1012240-mRNA-1
chr7_1 CDS 17318323 17318364 17318323 ID=Msa1012240-mRNA-1:cds;Parent=Msa1012240-mRNA-1
chr7_1 CDS 17316511 17316730 17316511 ID=Msa1012240-mRNA-1:cds;Parent=Msa1012240-mRNA-1
chr7_1 CDS 17315921 17316012 17315921 ID=Msa1012240-mRNA-1:cds;Parent=Msa1012240-mRNA-1
chr7_1 three_prime_UTR 17315535 17315920 17315535 ID=Msa1012240-mRNA-1:three_prime_utr;Parent=Msa1012240-mRNA-1
Gene Sequence

>Msa1012240

ATGAATTTGGTGGTGGAAGGTGGAAGAATGTGGTGGTTGGAGGTTTGTTATGATTGGAGCAACTACTGCAAAGATGGTGGATCTTGGTCGACAACATTCTCGCCTAAATCCACTGCAAAGCTGGTCAATGATAGTAACTTGCATAGTGGAGAAAATGAAACAGGGTTAGTGAGTAATCCTCAAGAGCTTCATGGGAAGTTGTCTGCTACTGGCAGTTCTAGCCGTGCCGTTGGACCTGTTGATCGTGTATCTACTCCAGCCGGGGCACATCAAGGAAGCATTTTTGCTAAAGATACCAGAAATCTGGCTGTTCAAGCAGCAGATCCCAGTTCTGAAGACAAAAGAACGCAGTGA
Protein sequence

>Msa1012240

MNLVVEGGRMWWLEVCYDWSNYCKDGGSWSTTFSPKSTAKLVNDSNLHSGENETGLVSNPQELHGKLSATGSSSRAVGPVDRVSTPAGAHQGSIFAKDTRNLAVQAADPSSEDKRTQ*