AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1012250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1012250 MtrunA17_Chr3g0138401 88.542 96 8 1 30 122 321 416 4.52e-52 169
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 38 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTTGAACAGCCAGATTTC+TGG 0.202017 7_1:+17327935 None:intergenic
TCTAAACCGGTCACCTTATC+AGG 0.216944 7_1:-17327628 Msa1012250:three_prime_UTR
ACCAGCTTTGCAGTGGATTT+AGG 0.291769 7_1:+17328626 None:intergenic
TGCTAAAGATACCAGAAATC+TGG 0.323635 7_1:-17327946 Msa1012250:CDS
GTCTACAGGTTTGTTATGAT+TGG 0.341574 7_1:-17328694 Msa1012250:intron
CATAGTGGAGAAAATGAAAC+AGG 0.343306 7_1:-17328587 Msa1012250:CDS
CAGAAATAAGAGAAAGCTCT+TGG 0.383667 7_1:+17327828 None:intergenic
TTCTATTGCAGTTTCTAAAC+CGG 0.384539 7_1:-17327641 Msa1012250:three_prime_UTR
CATGGGAAGTTGTCTGCTAC+TGG 0.416162 7_1:-17328539 Msa1012250:CDS
GCCTAAATCCACTGCAAAGC+TGG 0.430607 7_1:-17328627 Msa1012250:CDS
AGAAGATGCTTGACCTGATA+AGG 0.443032 7_1:+17327615 None:intergenic
GCTGCAGAATTGAAAGGAAC+AGG 0.452049 7_1:-17330699 Msa1012250:intron
GGAGTAGATACACGATCAAC+AGG 0.476638 7_1:+17328494 None:intergenic
ACTGCAAAGATGGTGGATCT+TGG 0.479375 7_1:-17328664 Msa1012250:CDS
GTGAGAAAATTTGACAAAAG+AGG 0.485887 7_1:+17327786 None:intergenic
AGAAATAAGAGAAAGCTCTT+GGG 0.490918 7_1:+17327829 None:intergenic
AGTAATCCTCAAGAGCTTCA+TGG 0.497229 7_1:-17328557 Msa1012250:CDS
TTTGAGATTTGCGATGGTTG+AGG 0.505284 7_1:-17330740 None:intergenic
GAGTAGGATGATTGTTAAAG+AGG 0.509074 7_1:-17327718 Msa1012250:three_prime_UTR
AACTTCCCATGAAGCTCTTG+AGG 0.518605 7_1:+17328551 None:intergenic
ATTATGTGGGGAGCAAGAGT+AGG 0.524042 7_1:-17327734 Msa1012250:three_prime_UTR
GTAATCCTCAAGAGCTTCAT+GGG 0.525373 7_1:-17328556 Msa1012250:CDS
AATAACAACCTTACGAATGT+TGG 0.526846 7_1:-17327580 Msa1012250:three_prime_UTR
ATCATTGACCAGCTTTGCAG+TGG 0.539773 7_1:+17328619 None:intergenic
GGCAGTTCTAGCCGTGCCGT+TGG 0.541164 7_1:-17328518 Msa1012250:CDS
TGGAGCAACTACTGCAAAGA+TGG 0.553115 7_1:-17328674 Msa1012250:CDS
TCTACTTACCAACATTCGTA+AGG 0.553647 7_1:+17327572 None:intergenic
GCTTGACCTGATAAGGTGAC+CGG 0.558454 7_1:+17327622 None:intergenic
AATGATAGTAACTTGCATAG+TGG 0.579416 7_1:-17328602 Msa1012250:CDS
ATGGTTGAGGCATTGTTCGA+AGG 0.580480 7_1:-17330727 Msa1012250:CDS
GTAGATGCTGCAGAATTGAA+AGG 0.586287 7_1:-17330705 Msa1012250:CDS
GATCGTGTATCTACTCCAGC+CGG 0.597972 7_1:-17328488 Msa1012250:intron
TACACGATCAACAGGTCCAA+CGG 0.603243 7_1:+17328502 None:intergenic
TAATTGATGTATCTGTTAGA+CGG 0.604298 7_1:+17327694 None:intergenic
GATCAACAGGTCCAACGGCA+CGG 0.605689 7_1:+17328507 None:intergenic
TGTATTGTAGGGGCACATCA+AGG 0.610537 7_1:-17327978 Msa1012250:intron
AGCAACTACTGCAAAGATGG+TGG 0.619072 7_1:-17328671 Msa1012250:CDS
ATAGTGGAGAAAATGAAACA+GGG 0.722474 7_1:-17328586 Msa1012250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAGTTTAATTTTCATTT+CGG + chr7_1:17328391-17328410 None:intergenic 10.0%
!!! CATAATCTTTTTTTTTTTTA+AGG + chr7_1:17330492-17330511 None:intergenic 10.0%
!! TAAAAAAAAAAAAGATTATG+TGG - chr7_1:17330492-17330511 Msa1012250:intron 10.0%
!! AAAAAAAAAAAAGATTATGT+GGG - chr7_1:17330493-17330512 Msa1012250:intron 10.0%
!!! AATTTTGTTTATTTCGAATT+CGG - chr7_1:17327840-17327859 Msa1012250:three_prime_UTR 15.0%
!!! AAAATGATTTTGTGATTTTT+TGG - chr7_1:17328157-17328176 Msa1012250:intron 15.0%
!!! TTTTAAAATGGATTTTTAAC+AGG - chr7_1:17328555-17328574 Msa1012250:CDS 15.0%
!!! TTTAAAATGGATTTTTAACA+GGG - chr7_1:17328556-17328575 Msa1012250:CDS 15.0%
!! TTTGCAAAATTCATATAAAA+GGG + chr7_1:17328991-17329010 None:intergenic 15.0%
!! TTCTAAATTCATTAGAATAT+TGG - chr7_1:17330074-17330093 Msa1012250:intron 15.0%
!!! TTTTTATGTTCATATTCAAT+GGG - chr7_1:17330385-17330404 Msa1012250:intron 15.0%
!! AAAAAAAAAAAGATTATGTG+GGG - chr7_1:17330494-17330513 Msa1012250:intron 15.0%
!!! AGAATGAATATAACAATTTT+TGG - chr7_1:17330707-17330726 Msa1012250:CDS 15.0%
!! CTTCAAATGCAAATTAAAAA+TGG - chr7_1:17328701-17328720 Msa1012250:intron 20.0%
!!! ATTGTTTGAGTCTTTTAAAT+TGG + chr7_1:17328924-17328943 None:intergenic 20.0%
!! GTTTGCAAAATTCATATAAA+AGG + chr7_1:17328992-17329011 None:intergenic 20.0%
!!! AGAATTGAACAAGTATTTTT+AGG - chr7_1:17329086-17329105 Msa1012250:intron 20.0%
!!! TATTTTTAGGTCCAAAATTA+GGG - chr7_1:17329099-17329118 Msa1012250:intron 20.0%
!! ACATAGAAAGTAAATAACTA+CGG + chr7_1:17329212-17329231 None:intergenic 20.0%
!!! ATAATTTGCTTGCTATTTTA+TGG - chr7_1:17329459-17329478 Msa1012250:intron 20.0%
!! AATTTCCCTTAATATGATAT+CGG - chr7_1:17329904-17329923 Msa1012250:intron 20.0%
!! TCTAATGAATTTAGAAAGAA+AGG + chr7_1:17330070-17330089 None:intergenic 20.0%
!! AATCAATAAAATATTCGAGT+TGG + chr7_1:17330130-17330149 None:intergenic 20.0%
!!! GTTTTTATGTTCATATTCAA+TGG - chr7_1:17330384-17330403 Msa1012250:intron 20.0%
!!! ACAATTTTTGGTTTAAAAAG+AGG - chr7_1:17330719-17330738 Msa1012250:CDS 20.0%
! TGAAAAGTTAGTTAGAAAAG+AGG - chr7_1:17328111-17328130 Msa1012250:intron 25.0%
! GAAAAGTTAGTTAGAAAAGA+GGG - chr7_1:17328112-17328131 Msa1012250:intron 25.0%
!! TGCAAATAACAAAAGTACAA+AGG - chr7_1:17328365-17328384 Msa1012250:intron 25.0%
!!! ACTCAGATGCAATTTTAAAA+TGG - chr7_1:17328543-17328562 Msa1012250:CDS 25.0%
! ACTTATTCAAACTATTTGAG+AGG - chr7_1:17328749-17328768 Msa1012250:intron 25.0%
!!! TCAAACAAGTTCTAAAAGAT+TGG - chr7_1:17328774-17328793 Msa1012250:intron 25.0%
!! ATTGGAAGAGATTTTATCAT+GGG - chr7_1:17328792-17328811 Msa1012250:intron 25.0%
!! AAAGACTCAAACAATTTTCA+AGG - chr7_1:17328929-17328948 Msa1012250:intron 25.0%
! AATGACTTATAATGCAAGAA+AGG - chr7_1:17329062-17329081 Msa1012250:intron 25.0%
!!! GTATTTTTAGGTCCAAAATT+AGG - chr7_1:17329098-17329117 Msa1012250:intron 25.0%
!!! TTTGTTTCTAGCTTTATAGT+TGG - chr7_1:17329248-17329267 Msa1012250:intron 25.0%
! AATTATGGTAACTGAATTGA+AGG + chr7_1:17329500-17329519 None:intergenic 25.0%
! GTATTATAATACCTCCAAAT+TGG - chr7_1:17329827-17329846 Msa1012250:intron 25.0%
!! TGATTGATCAAAGCTAATTT+TGG + chr7_1:17329950-17329969 None:intergenic 25.0%
!!! ATAAAATATTCGAGTTGGTA+CGG + chr7_1:17330125-17330144 None:intergenic 25.0%
!! AGAAGACAAGAATTTTCATA+TGG + chr7_1:17330169-17330188 None:intergenic 25.0%
! TGAAAATTCTTGTCTTCTTT+AGG - chr7_1:17330171-17330190 Msa1012250:intron 25.0%
! TAATTGATGTATCTGTTAGA+CGG + chr7_1:17330549-17330568 None:intergenic 25.0%
!!! GCAATAGAAGATGTTTTTTA+CGG + chr7_1:17330588-17330607 None:intergenic 25.0%
!!! CTTTTGATTTTGTGTTGTGA+TGG - chr7_1:17327881-17327900 Msa1012250:exon 30.0%
TTGTGGTTATGTGATGAATT+TGG - chr7_1:17327907-17327926 Msa1012250:CDS 30.0%
AAAAGAGGGAAAAAATTGAG+AGG - chr7_1:17328126-17328145 Msa1012250:intron 30.0%
! ACCAAGACTTTGACTTATAT+AGG - chr7_1:17328215-17328234 Msa1012250:intron 30.0%
ACCTATATAAGTCAAAGTCT+TGG + chr7_1:17328219-17328238 None:intergenic 30.0%
TGACTTATATAGGTGATGAA+AGG - chr7_1:17328225-17328244 Msa1012250:intron 30.0%
GACTTATATAGGTGATGAAA+GGG - chr7_1:17328226-17328245 Msa1012250:intron 30.0%
!! TGACTTGTAACAAACTTTTG+TGG - chr7_1:17328284-17328303 Msa1012250:intron 30.0%
GGACTCTAACAAACTAAAAA+AGG - chr7_1:17328335-17328354 Msa1012250:intron 30.0%
! GATTGGAAGAGATTTTATCA+TGG - chr7_1:17328791-17328810 Msa1012250:intron 30.0%
TTTGACATGAGATTGAAACA+AGG - chr7_1:17328887-17328906 Msa1012250:intron 30.0%
! GATTAAAATGAGTTGAATGC+AGG - chr7_1:17328951-17328970 Msa1012250:intron 30.0%
TCATTATTGAATGTACTGCA+GGG + chr7_1:17329140-17329159 None:intergenic 30.0%
TGCAGTACATTCAATAATGA+CGG - chr7_1:17329140-17329159 Msa1012250:intron 30.0%
ATTAGATATTTGAGCCTTGA+GGG - chr7_1:17329384-17329403 Msa1012250:intron 30.0%
! GTTGTTTCAGAGTCAAATTA+TGG + chr7_1:17329515-17329534 None:intergenic 30.0%
! AATGATAGTAACTTGCATAG+TGG - chr7_1:17329638-17329657 Msa1012250:intron 30.0%
! ATAGTGGAGAAAATGAAACA+GGG - chr7_1:17329654-17329673 Msa1012250:intron 30.0%
TCTTTCAATCACTACCAATT+TGG + chr7_1:17329844-17329863 None:intergenic 30.0%
GTTGTCCGATATCATATTAA+GGG + chr7_1:17329912-17329931 None:intergenic 30.0%
AGTTGTCCGATATCATATTA+AGG + chr7_1:17329913-17329932 None:intergenic 30.0%
!! TAAAATATTCGAGTTGGTAC+GGG + chr7_1:17330124-17330143 None:intergenic 30.0%
TCGATTGATTGCTAAATATC+CGG - chr7_1:17330238-17330257 Msa1012250:intron 30.0%
TAAATATCCGGTTGTATTGT+AGG - chr7_1:17330250-17330269 Msa1012250:intron 30.0%
AAATATCCGGTTGTATTGTA+GGG - chr7_1:17330251-17330270 Msa1012250:intron 30.0%
AGAAATAAGAGAAAGCTCTT+GGG + chr7_1:17330414-17330433 None:intergenic 30.0%
GTGAGAAAATTTGACAAAAG+AGG + chr7_1:17330457-17330476 None:intergenic 30.0%
TTCTATTGCAGTTTCTAAAC+CGG - chr7_1:17330599-17330618 Msa1012250:intron 30.0%
AATAACAACCTTACGAATGT+TGG - chr7_1:17330660-17330679 Msa1012250:intron 30.0%
AAGAGGATTGAAGATGATTG+CGG - chr7_1:17327596-17327615 Msa1012250:three_prime_UTR 35.0%
TGCTTCTTCGAATTGAGAAT+TGG - chr7_1:17327677-17327696 Msa1012250:three_prime_UTR 35.0%
GCTTCTTCGAATTGAGAATT+GGG - chr7_1:17327678-17327697 Msa1012250:three_prime_UTR 35.0%
!!! TCGAATTCGGTTTTGAACAA+CGG - chr7_1:17327853-17327872 Msa1012250:three_prime_UTR 35.0%
! TTGTGTTGTGATGGTTTTTG+TGG - chr7_1:17327890-17327909 Msa1012250:exon 35.0%
TGGTTATGTGATGAATTTGG+TGG - chr7_1:17327910-17327929 Msa1012250:CDS 35.0%
! TTATGTGATGAATTTGGTGG+TGG - chr7_1:17327913-17327932 Msa1012250:CDS 35.0%
!! AAGGTGGTTAGAGTTTGTTA+GGG - chr7_1:17328054-17328073 Msa1012250:intron 35.0%
AGAGGATGAGAAAGTTAGTT+AGG - chr7_1:17328084-17328103 Msa1012250:intron 35.0%
GAGGATGAGAAAGTTAGTTA+GGG - chr7_1:17328085-17328104 Msa1012250:intron 35.0%
ACCAATGAAATAATGCCATG+TGG - chr7_1:17328257-17328276 Msa1012250:intron 35.0%
! TTTTGTGGACCAACAACATT+TGG - chr7_1:17328299-17328318 Msa1012250:intron 35.0%
!! TTTAGTCGTTTTAGTCCCTA+GGG + chr7_1:17328519-17328538 None:intergenic 35.0%
!! TTTTAGTCGTTTTAGTCCCT+AGG + chr7_1:17328520-17328539 None:intergenic 35.0%
AAAAGACTAAAATGCCCCTA+GGG - chr7_1:17328658-17328677 Msa1012250:CDS 35.0%
!!! GGCTTGTTATACCCTAATTT+TGG + chr7_1:17329113-17329132 None:intergenic 35.0%
GTCATTATTGAATGTACTGC+AGG + chr7_1:17329141-17329160 None:intergenic 35.0%
AAACCCTTCATACTTATCGA+TGG - chr7_1:17329288-17329307 Msa1012250:intron 35.0%
GCTCAAATATCTAATCCAAG+TGG + chr7_1:17329379-17329398 None:intergenic 35.0%
GATTAGATATTTGAGCCTTG+AGG - chr7_1:17329383-17329402 Msa1012250:intron 35.0%
AATTGACAGTTTAACCCTCA+AGG + chr7_1:17329401-17329420 None:intergenic 35.0%
! AATTGAAGGCACATATCAAC+AGG + chr7_1:17329486-17329505 None:intergenic 35.0%
! AACTTTTACACATCGTCTAC+AGG - chr7_1:17329532-17329551 Msa1012250:intron 35.0%
!! GTCTACAGGTTTGTTATGAT+TGG - chr7_1:17329546-17329565 Msa1012250:intron 35.0%
! CATAGTGGAGAAAATGAAAC+AGG - chr7_1:17329653-17329672 Msa1012250:intron 35.0%
GCATACTGCATAGTTTGATT+TGG - chr7_1:17329785-17329804 Msa1012250:intron 35.0%
TTCAATCACTACCAATTTGG+AGG + chr7_1:17329841-17329860 None:intergenic 35.0%
! TGGTAGTGATTGAAAGACTA+CGG - chr7_1:17329847-17329866 Msa1012250:intron 35.0%
TTAAGGGAAATTACTACTGC+AGG + chr7_1:17329896-17329915 None:intergenic 35.0%
AAGAAAAGGATGACACGAAA+AGG + chr7_1:17330036-17330055 None:intergenic 35.0%
! CATCCTTTTCTTTACCTTAC+TGG - chr7_1:17330044-17330063 Msa1012250:intron 35.0%
TTTAGAAAGAAAGGCCAGTA+AGG + chr7_1:17330061-17330080 None:intergenic 35.0%
AATATCCGGTTGTATTGTAG+GGG - chr7_1:17330252-17330271 Msa1012250:intron 35.0%
TGCTAAAGATACCAGAAATC+TGG - chr7_1:17330294-17330313 Msa1012250:intron 35.0%
! GTTCTTTTGTCTTCAGAACT+GGG + chr7_1:17330337-17330356 None:intergenic 35.0%
CAGAAATAAGAGAAAGCTCT+TGG + chr7_1:17330415-17330434 None:intergenic 35.0%
GAGTAGGATGATTGTTAAAG+AGG - chr7_1:17330522-17330541 Msa1012250:intron 35.0%
TCTACTTACCAACATTCGTA+AGG + chr7_1:17330671-17330690 None:intergenic 35.0%
GTAGATGCTGCAGAATTGAA+AGG - chr7_1:17327535-17327554 Msa1012250:three_prime_UTR 40.0%
!! AATGGATTGAACAAGAGCGT+CGG - chr7_1:17327573-17327592 Msa1012250:three_prime_UTR 40.0%
!!! GTTGCAGCTCTGTTTTGATA+CGG - chr7_1:17327707-17327726 Msa1012250:three_prime_UTR 40.0%
!! GTTCTGAACTTGTGTGCTTT+TGG - chr7_1:17327814-17327833 Msa1012250:three_prime_UTR 40.0%
ATGAAGAAAGTGGAGGTTGA+AGG - chr7_1:17328022-17328041 Msa1012250:intron 40.0%
! GAAGGTGGTTAGAGTTTGTT+AGG - chr7_1:17328053-17328072 Msa1012250:intron 40.0%
GATGAGAAAGTTAGTTAGGG+TGG - chr7_1:17328088-17328107 Msa1012250:intron 40.0%
GCCACATGGCATTATTTCAT+TGG + chr7_1:17328261-17328280 None:intergenic 40.0%
!! TTAGTCGTTTTAGTCCCTAG+GGG + chr7_1:17328518-17328537 None:intergenic 40.0%
GAAAAGACTAAAATGCCCCT+AGG - chr7_1:17328657-17328676 Msa1012250:CDS 40.0%
AAAGACTAAAATGCCCCTAG+GGG - chr7_1:17328659-17328678 Msa1012250:CDS 40.0%
AAATGACCAAAATGCCCCTA+GGG - chr7_1:17328815-17328834 Msa1012250:intron 40.0%
TTTAGTCAGTTTAGCCCCTA+GGG + chr7_1:17328833-17328852 None:intergenic 40.0%
! TTTTAGTCAGTTTAGCCCCT+AGG + chr7_1:17328834-17328853 None:intergenic 40.0%
TTGAATGTACTGCAGGGTAT+GGG + chr7_1:17329134-17329153 None:intergenic 40.0%
ATTGAATGTACTGCAGGGTA+TGG + chr7_1:17329135-17329154 None:intergenic 40.0%
GGACCATCGATAAGTATGAA+GGG + chr7_1:17329294-17329313 None:intergenic 40.0%
AGGACCATCGATAAGTATGA+AGG + chr7_1:17329295-17329314 None:intergenic 40.0%
! CATGATGTTTCTGCGAAATG+AGG + chr7_1:17329315-17329334 None:intergenic 40.0%
TATCTAATCCAAGTGGCAGA+TGG + chr7_1:17329372-17329391 None:intergenic 40.0%
AGTAATCCTCAAGAGCTTCA+TGG - chr7_1:17329683-17329702 Msa1012250:intron 40.0%
GTAATCCTCAAGAGCTTCAT+GGG - chr7_1:17329684-17329703 Msa1012250:intron 40.0%
CAAACTATGCAGTATGCTTG+AGG + chr7_1:17329782-17329801 None:intergenic 40.0%
!! TACTGCAGGCGATATTTTTG+GGG + chr7_1:17329882-17329901 None:intergenic 40.0%
! CTACTGCAGGCGATATTTTT+GGG + chr7_1:17329883-17329902 None:intergenic 40.0%
! ACTACTGCAGGCGATATTTT+TGG + chr7_1:17329884-17329903 None:intergenic 40.0%
! ACGAAAAGGATCAGTGGATT+TGG + chr7_1:17330022-17330041 None:intergenic 40.0%
AGGCCAGTAAGGTAAAGAAA+AGG + chr7_1:17330050-17330069 None:intergenic 40.0%
! CGTTCTTTTGTCTTCAGAAC+TGG + chr7_1:17330338-17330357 None:intergenic 40.0%
AGAAGATGCTTGACCTGATA+AGG + chr7_1:17330628-17330647 None:intergenic 40.0%
!! ATGGTTGAGGCATTGTTCGA+AGG - chr7_1:17327513-17327532 Msa1012250:three_prime_UTR 45.0%
GCTGCAGAATTGAAAGGAAC+AGG - chr7_1:17327541-17327560 Msa1012250:three_prime_UTR 45.0%
AGGAACAGGTCTGTGATGAA+TGG - chr7_1:17327555-17327574 Msa1012250:three_prime_UTR 45.0%
TCGTCGCAGAAGCTTGAAAT+TGG - chr7_1:17327631-17327650 Msa1012250:three_prime_UTR 45.0%
! TTCCGGTAACGGCAATCTTA+CGG + chr7_1:17327770-17327789 None:intergenic 45.0%
AAGCCTCAAGTTTCCGGTAA+CGG + chr7_1:17327781-17327800 None:intergenic 45.0%
TTACCGGAAACTTGAGGCTT+CGG - chr7_1:17327781-17327800 Msa1012250:three_prime_UTR 45.0%
!! GTGATGAATTTGGTGGTGGA+AGG - chr7_1:17327917-17327936 Msa1012250:CDS 45.0%
!! ATGAATTTGGTGGTGGAAGG+TGG - chr7_1:17327920-17327939 Msa1012250:CDS 45.0%
! GGTTGTTAGGTGGTGGTTTA+TGG - chr7_1:17327961-17327980 Msa1012250:CDS 45.0%
! TGTTAGGTGGTGGTTTATGG+TGG - chr7_1:17327964-17327983 Msa1012250:CDS 45.0%
AGGTTTGTGGTGGTTGTTAG+AGG - chr7_1:17327993-17328012 Msa1012250:intron 45.0%
TTGTGGTGGTTGTTAGAGGT+TGG - chr7_1:17327997-17328016 Msa1012250:intron 45.0%
GAGGTTGGAGATGAAGAAAG+TGG - chr7_1:17328012-17328031 Msa1012250:intron 45.0%
GTTGGAGATGAAGAAAGTGG+AGG - chr7_1:17328015-17328034 Msa1012250:intron 45.0%
AAGAAAGTGGAGGTTGAAGG+TGG - chr7_1:17328025-17328044 Msa1012250:intron 45.0%
!! GTGGTTAGAGTTTGTTAGGG+TGG - chr7_1:17328057-17328076 Msa1012250:intron 45.0%
! GTTTGTTAGGGTGGAGATAG+AGG - chr7_1:17328066-17328085 Msa1012250:intron 45.0%
TAGGTGATGAAAGGGATGCT+TGG - chr7_1:17328234-17328253 Msa1012250:intron 45.0%
GTTACAAGTCAAGTGCCACA+TGG + chr7_1:17328275-17328294 None:intergenic 45.0%
ACCAACAACATTTGGACACG+TGG - chr7_1:17328307-17328326 Msa1012250:intron 45.0%
AAATGACCAAAACGCCCCTA+GGG - chr7_1:17328501-17328520 Msa1012250:CDS 45.0%
! TTGAGTCAGTTTTGCCCCTA+GGG + chr7_1:17328676-17328695 None:intergenic 45.0%
! TTTGAGTCAGTTTTGCCCCT+AGG + chr7_1:17328677-17328696 None:intergenic 45.0%
GAAATGACCAAAATGCCCCT+AGG - chr7_1:17328814-17328833 Msa1012250:intron 45.0%
AATGACCAAAATGCCCCTAG+GGG - chr7_1:17328816-17328835 Msa1012250:intron 45.0%
TTAGTCAGTTTAGCCCCTAG+GGG + chr7_1:17328832-17328851 None:intergenic 45.0%
CGGTTCCATATATGCTTACG+CGG - chr7_1:17329160-17329179 Msa1012250:intron 45.0%
GCATACCGCGTAAGCATATA+TGG + chr7_1:17329168-17329187 None:intergenic 45.0%
TGGAGCAACTACTGCAAAGA+TGG - chr7_1:17329566-17329585 Msa1012250:intron 45.0%
AGCAACTACTGCAAAGATGG+TGG - chr7_1:17329569-17329588 Msa1012250:intron 45.0%
!! ACTGCAAAGATGGTGGATCT+TGG - chr7_1:17329576-17329595 Msa1012250:intron 45.0%
! ACCAGCTTTGCAGTGGATTT+AGG + chr7_1:17329617-17329636 None:intergenic 45.0%
ATCATTGACCAGCTTTGCAG+TGG + chr7_1:17329624-17329643 None:intergenic 45.0%
AACTTCCCATGAAGCTCTTG+AGG + chr7_1:17329692-17329711 None:intergenic 45.0%
TACACGATCAACAGGTCCAA+CGG + chr7_1:17329741-17329760 None:intergenic 45.0%
GGAGTAGATACACGATCAAC+AGG + chr7_1:17329749-17329768 None:intergenic 45.0%
GCAGTATGCTTGAGGATTAC+CGG + chr7_1:17329774-17329793 None:intergenic 45.0%
GATGACACGAAAAGGATCAG+TGG + chr7_1:17330028-17330047 None:intergenic 45.0%
ATGTGCCCCTACAATACAAC+CGG + chr7_1:17330260-17330279 None:intergenic 45.0%
! TGTATTGTAGGGGCACATCA+AGG - chr7_1:17330262-17330281 Msa1012250:intron 45.0%
TGCTTGAACAGCCAGATTTC+TGG + chr7_1:17330308-17330327 None:intergenic 45.0%
ATTATGTGGGGAGCAAGAGT+AGG - chr7_1:17330506-17330525 Msa1012250:intron 45.0%
TCTAAACCGGTCACCTTATC+AGG - chr7_1:17330612-17330631 Msa1012250:intron 45.0%
!! TTGAACAAGAGCGTCGGAAG+AGG - chr7_1:17327579-17327598 Msa1012250:three_prime_UTR 50.0%
ACGGTGAAGCAAGAACGTGA+AGG + chr7_1:17327751-17327770 None:intergenic 50.0%
CACCGTAAGATTGCCGTTAC+CGG - chr7_1:17327765-17327784 Msa1012250:three_prime_UTR 50.0%
TTGCCGTTACCGGAAACTTG+AGG - chr7_1:17327775-17327794 Msa1012250:three_prime_UTR 50.0%
AAGCCGAAGCCTCAAGTTTC+CGG + chr7_1:17327787-17327806 None:intergenic 50.0%
TGGTGGAAGGTGGAAGAATG+TGG - chr7_1:17327930-17327949 Msa1012250:CDS 50.0%
TGGAAGGTGGAAGAATGTGG+TGG - chr7_1:17327933-17327952 Msa1012250:CDS 50.0%
AGGTGGAAGAATGTGGTGGT+TGG - chr7_1:17327937-17327956 Msa1012250:CDS 50.0%
TGGAAGAATGTGGTGGTTGG+AGG - chr7_1:17327940-17327959 Msa1012250:CDS 50.0%
TGTGGTGGTTGGAGGTTGTT+AGG - chr7_1:17327948-17327967 Msa1012250:CDS 50.0%
GTGGTGGTTTATGGTGGTTG+TGG - chr7_1:17327970-17327989 Msa1012250:CDS 50.0%
GTGGTTTATGGTGGTTGTGG+AGG - chr7_1:17327973-17327992 Msa1012250:intron 50.0%
ATGGTGGTTGTGGAGGTTTG+TGG - chr7_1:17327980-17327999 Msa1012250:intron 50.0%
AAAGTGGAGGTTGAAGGTGG+TGG - chr7_1:17328028-17328047 Msa1012250:intron 50.0%
! AGGTTGAAGGTGGTGGTTGA+AGG - chr7_1:17328035-17328054 Msa1012250:intron 50.0%
! TTGAAGGTGGTGGTTGAAGG+TGG - chr7_1:17328038-17328057 Msa1012250:intron 50.0%
GCCACGTGTCCAAATGTTGT+TGG + chr7_1:17328311-17328330 None:intergenic 50.0%
ACATTTGGACACGTGGCACT+TGG - chr7_1:17328314-17328333 Msa1012250:intron 50.0%
GAAATGACCAAAACGCCCCT+AGG - chr7_1:17328500-17328519 Msa1012250:CDS 50.0%
!!! TTTAGTCCCTAGGGGCGTTT+TGG + chr7_1:17328510-17328529 None:intergenic 50.0%
! TGAGTCAGTTTTGCCCCTAG+GGG + chr7_1:17328675-17328694 None:intergenic 50.0%
!!! TTTAGCCCCTAGGGGCATTT+TGG + chr7_1:17328824-17328843 None:intergenic 50.0%
GCTTAGATCCATCTGCCACT+TGG - chr7_1:17329361-17329380 Msa1012250:intron 50.0%
GCCTAAATCCACTGCAAAGC+TGG - chr7_1:17329613-17329632 Msa1012250:intron 50.0%
CATGGGAAGTTGTCTGCTAC+TGG - chr7_1:17329701-17329720 Msa1012250:intron 50.0%
GATCGTGTATCTACTCCAGC+CGG - chr7_1:17329752-17329771 Msa1012250:intron 50.0%
TATGCTTGAGGATTACCGGC+TGG + chr7_1:17329770-17329789 None:intergenic 50.0%
TTGGTACGGGCAAGGTCTAA+AGG + chr7_1:17330111-17330130 None:intergenic 50.0%
!! TATTCGAGTTGGTACGGGCA+AGG + chr7_1:17330119-17330138 None:intergenic 50.0%
GCTTGACCTGATAAGGTGAC+CGG + chr7_1:17330621-17330640 None:intergenic 50.0%
GGTGGTTGGAGGTTGTTAGG+TGG - chr7_1:17327951-17327970 Msa1012250:CDS 55.0%
GGTTGGAGGTTGTTAGGTGG+TGG - chr7_1:17327954-17327973 Msa1012250:CDS 55.0%
GTGGTTGTGGAGGTTTGTGG+TGG - chr7_1:17327983-17328002 Msa1012250:intron 55.0%
GATCAACAGGTCCAACGGCA+CGG + chr7_1:17329736-17329755 None:intergenic 55.0%
! GGCAGTTCTAGCCGTGCCGT+TGG - chr7_1:17329722-17329741 Msa1012250:intron 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 17327513 17330749 17327513 ID=Msa1012250;Name=Msa1012250
chr7_1 mRNA 17327513 17330749 17327513 ID=Msa1012250-mRNA-1;Parent=Msa1012250;Name=Msa1012250-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0.33|0.25|0.5|0.33|0.5|4|386|122
chr7_1 exon 17327513 17327990 17327513 ID=Msa1012250-mRNA-1:exon:4351;Parent=Msa1012250-mRNA-1
chr7_1 exon 17328489 17328708 17328489 ID=Msa1012250-mRNA-1:exon:4350;Parent=Msa1012250-mRNA-1
chr7_1 exon 17328997 17329003 17328997 ID=Msa1012250-mRNA-1:exon:4349;Parent=Msa1012250-mRNA-1
chr7_1 exon 17330700 17330749 17330700 ID=Msa1012250-mRNA-1:exon:4348;Parent=Msa1012250-mRNA-1
chr7_1 CDS 17330700 17330749 17330700 ID=Msa1012250-mRNA-1:cds;Parent=Msa1012250-mRNA-1
chr7_1 CDS 17328997 17329003 17328997 ID=Msa1012250-mRNA-1:cds;Parent=Msa1012250-mRNA-1
chr7_1 CDS 17328489 17328708 17328489 ID=Msa1012250-mRNA-1:cds;Parent=Msa1012250-mRNA-1
chr7_1 CDS 17327899 17327990 17327899 ID=Msa1012250-mRNA-1:cds;Parent=Msa1012250-mRNA-1
chr7_1 three_prime_UTR 17327513 17327898 17327513 ID=Msa1012250-mRNA-1:three_prime_utr;Parent=Msa1012250-mRNA-1
Gene Sequence

>Msa1012250

ATGGTTGAGGCATTGTTCGAAGGTAGATGCTGCAGAATTGAAAGGAACAGCTTTATAGTTTGTTATGATTGGAGCAACTACTGCAAAGATGGTGGATCTTGGTCGACAACATTCTCGCCTAAATCCACTGCAAAGCTGGTCAATGATAGTAACTTGCATAGTGGAGAAAATGAAACAGGGTTAGTGAGTAATCCTCAAGAGCTTCATGGGAAGTTGTCTGCTACTGGCAGTTCTAGCCGTGCCGTTGGACCTGTTGATCGTGTATCTACTCCAGCCGGGGCACATCAAGGAAGCATTTTTGCTAAAGATACCAGAAATCTGGCTGTTCAAGCAGCAGATCCCAGTTCTGAAGACAAAAGAACGCAGTGA
Protein sequence

>Msa1012250

MVEALFEGRCCRIERNSFIVCYDWSNYCKDGGSWSTTFSPKSTAKLVNDSNLHSGENETGLVSNPQELHGKLSATGSSSRAVGPVDRVSTPAGAHQGSIFAKDTRNLAVQAADPSSEDKRTQ*