AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1297380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1297380 MtrunA17_Chr4g0054581 93.158 380 26 0 1 380 1 380 0.0 662
Msa1297380 MtrunA17_Chr2g0307131 60.309 388 111 11 1 378 1 355 3.67e-135 390
Msa1297380 MtrunA17_Chr2g0307141 58.205 390 124 10 1 380 1 361 7.79e-126 367
Msa1297380 MtrunA17_Chr6g0468491 55.890 365 101 7 24 379 1 314 1.50e-122 356
Msa1297380 MtrunA17_Chr4g0033391 53.109 386 105 10 1 377 1 319 3.37e-115 338
Msa1297380 MtrunA17_Chr4g0054621 51.139 395 124 14 1 379 1 342 7.37e-110 325
Msa1297380 MtrunA17_Chr4g0033291 53.351 388 113 10 1 379 1 329 8.44e-107 317
Msa1297380 MtrunA17_Chr6g0468461 52.941 374 106 11 1 362 1 316 3.19e-106 316
Msa1297380 MtrunA17_Chr4g0033281 51.026 390 120 9 1 381 1 328 1.23e-104 311
Msa1297380 MtrunA17_Chr7g0225901 45.646 333 86 9 24 354 1 240 6.76e-73 228
Msa1297380 MtrunA17_Chr5g0400621 52.124 259 97 6 121 378 101 333 3.33e-68 218
Msa1297380 MtrunA17_Chr4g0002801 51.064 188 88 3 191 375 145 331 2.42e-55 185
Msa1297380 MtrunA17_Chr2g0280161 51.075 186 80 4 196 377 175 353 1.19e-54 184
Msa1297380 MtrunA17_Chr5g0400661 36.870 377 175 14 13 374 6 334 7.14e-54 181
Msa1297380 MtrunA17_Chr5g0400641 54.601 163 64 3 201 360 1 156 1.19e-49 164
Msa1297380 MtrunA17_Chr4g0002781 40.449 267 123 5 116 375 113 350 2.10e-49 170
Msa1297380 MtrunA17_Chr2g0333641 41.065 263 127 7 108 369 67 302 2.71e-49 168
Msa1297380 MtrunA17_Chr8g0336681 39.338 272 123 8 111 370 90 331 5.56e-46 160
Msa1297380 MtrunA17_Chr4g0049331 45.960 198 99 4 182 377 119 310 5.27e-45 157
Msa1297380 MtrunA17_Chr4g0054561 47.872 188 86 6 185 369 142 320 6.73e-45 157
Msa1297380 MtrunA17_Chr2g0333621 39.194 273 139 7 108 378 68 315 8.72e-45 157
Msa1297380 MtrunA17_Chr4g0054551 46.277 188 89 6 185 369 142 320 2.35e-44 156
Msa1297380 MtrunA17_Chr4g0054571 38.438 320 153 12 69 376 44 331 1.17e-43 154
Msa1297380 MtrunA17_Chr5g0400671 34.441 331 159 13 68 374 27 323 1.27e-40 146
Msa1297380 MtrunA17_Chr2g0333611 38.532 218 108 7 167 376 18 217 1.35e-39 140
Msa1297380 MtrunA17_Chr2g0333541 40.092 217 126 2 159 373 53 267 1.77e-39 142
Msa1297380 MtrunA17_Chr7g0249811 31.231 333 179 8 74 382 26 332 3.35e-37 137
Msa1297380 MtrunA17_Chr4g0002831 41.975 162 61 5 221 372 64 202 2.66e-28 110
Msa1297380 MtrunA17_Chr7g0233441 39.752 161 65 4 221 372 57 194 2.30e-26 104
Msa1297380 MtrunA17_Chr4g0002851 38.125 160 61 4 221 372 65 194 1.60e-24 99.8
Msa1297380 MtrunA17_Chr1g0185621 31.492 181 113 4 198 369 475 653 4.72e-18 85.9
Msa1297380 MtrunA17_Chr8g0366751 36.585 164 88 4 190 339 448 609 4.87e-18 85.9
Msa1297380 MtrunA17_Chr8g0346161 30.405 148 87 4 198 339 291 428 1.00e-16 81.6
Msa1297380 MtrunA17_Chr5g0411341 31.522 184 102 4 190 355 479 656 7.28e-15 76.3
Msa1297380 MtrunA17_Chr7g0275821 53.571 56 26 0 198 253 252 307 6.06e-14 72.8
Msa1297380 MtrunA17_Chr7g0260841 47.059 68 30 1 190 251 341 408 4.91e-13 70.5
Msa1297380 MtrunA17_Chr8g0346151 33.051 118 70 4 198 311 279 391 1.72e-12 68.6
Msa1297380 MtrunA17_Chr8g0365711 25.595 168 112 5 191 351 237 398 1.98e-12 68.2
Msa1297380 MtrunA17_Chr1g0211081 25.434 173 106 3 190 339 309 481 2.34e-12 68.2
Msa1297380 MtrunA17_Chr3g0131691 27.097 155 108 1 183 332 283 437 2.47e-12 68.2
Msa1297380 MtrunA17_Chr7g0270871 46.875 64 34 0 187 250 201 264 2.87e-11 64.3
Msa1297380 MtrunA17_Chr1g0153241 36.538 104 63 2 153 254 49 151 4.41e-11 62.8
Msa1297380 MtrunA17_Chr7g0252041 26.471 170 111 4 199 357 287 453 4.42e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1297380 AT4G37850.2 38.966 290 141 10 101 377 62 328 4.05e-51 174
Msa1297380 AT4G37850.1 38.966 290 141 10 101 377 62 328 4.05e-51 174
Msa1297380 AT2G22750.2 46.154 195 104 1 183 376 110 304 2.39e-44 155
Msa1297380 AT2G22750.3 37.037 378 182 16 7 376 3 332 2.72e-44 156
Msa1297380 AT2G22750.5 46.429 196 102 3 183 376 134 328 3.57e-44 156
Msa1297380 AT2G22750.1 46.154 195 103 2 183 376 110 303 6.62e-43 152
Msa1297380 AT2G22750.4 37.037 378 181 17 7 376 3 331 8.65e-43 152
Msa1297380 AT2G22760.1 42.487 193 103 5 186 374 107 295 8.80e-41 146
Msa1297380 AT2G22760.2 43.023 172 91 5 186 354 107 274 2.84e-34 128
Msa1297380 AT2G22770.1 32.915 319 141 11 82 372 44 317 1.53e-30 119
Msa1297380 AT4G17880.1 32.000 175 102 3 190 355 402 568 4.82e-16 80.1
Msa1297380 AT1G32640.1 33.766 154 82 4 190 332 438 582 7.90e-16 79.7
Msa1297380 AT5G46760.1 27.179 195 113 5 190 358 401 592 1.95e-15 78.2
Msa1297380 AT2G46510.1 47.297 74 33 1 190 257 381 454 5.72e-15 77.0
Msa1297380 AT1G01260.1 36.066 122 51 3 192 313 427 521 6.83e-15 76.6
Msa1297380 AT1G01260.2 36.066 122 51 3 192 313 427 521 6.83e-15 76.6
Msa1297380 AT1G01260.3 36.066 122 51 3 192 313 427 521 6.83e-15 76.6
Msa1297380 AT4G16430.1 49.254 67 34 0 192 258 314 380 4.68e-14 73.9
Msa1297380 AT1G63650.3 28.571 154 85 4 198 332 405 552 3.29e-13 71.6
Msa1297380 AT1G63650.1 28.571 154 85 4 198 332 405 552 3.29e-13 71.6
Msa1297380 AT1G63650.2 28.571 154 85 4 198 332 405 552 3.29e-13 71.6
Msa1297380 AT2G16910.1 28.659 164 94 4 199 342 315 475 1.77e-12 69.3
Msa1297380 AT4G09820.1 29.375 160 74 4 189 339 352 481 2.33e-12 68.6
Msa1297380 AT3G26744.1 27.879 165 113 2 199 357 308 472 3.21e-12 68.2
Msa1297380 AT3G26744.4 27.879 165 113 2 199 357 308 472 3.21e-12 68.2
Msa1297380 AT3G26744.2 27.879 165 113 2 199 357 308 472 3.21e-12 68.2
Msa1297380 AT5G41315.3 25.126 199 120 5 192 367 440 632 3.26e-12 68.6
Msa1297380 AT5G41315.1 25.126 199 120 5 192 367 435 627 3.42e-12 68.6
Msa1297380 AT5G41315.2 25.126 199 120 5 192 367 443 635 3.48e-12 68.6
Msa1297380 AT4G00870.1 32.800 125 64 2 199 314 250 363 5.21e-12 67.4
Msa1297380 AT3G26744.6 28.777 139 94 1 199 332 308 446 8.85e-12 67.0
Msa1297380 AT5G57150.3 28.402 169 107 4 196 355 53 216 2.07e-11 63.5
Msa1297380 AT2G28160.2 28.743 167 101 5 179 340 117 270 2.44e-11 64.7
Msa1297380 AT2G28160.1 28.743 167 101 5 179 340 117 270 2.54e-11 64.7
Msa1297380 AT4G29930.1 26.286 175 111 4 199 356 55 228 3.00e-11 63.5
Msa1297380 AT4G29930.6 26.415 159 105 3 199 356 55 202 3.36e-11 63.2
Msa1297380 AT5G57150.2 28.571 168 106 4 196 354 53 215 3.46e-11 63.2
Msa1297380 AT4G29930.3 26.012 173 110 4 199 354 55 226 3.63e-11 63.5
Msa1297380 AT4G29930.7 26.012 173 110 4 199 354 55 226 3.63e-11 63.5
Msa1297380 AT5G57150.1 28.824 170 107 4 196 356 52 216 3.74e-11 63.2
Msa1297380 AT4G29930.2 25.786 159 103 2 199 356 55 199 3.86e-11 62.8
Msa1297380 AT5G57150.5 28.571 168 106 4 196 354 52 214 4.15e-11 62.8
Msa1297380 AT5G57150.4 28.571 168 106 4 196 354 53 215 4.51e-11 63.2
Msa1297380 AT4G29930.5 24.841 157 103 2 199 354 55 197 7.09e-11 62.4

Find 88 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGAATCCCCTTGTGCTTC+TGG 0.171911 8_3:+57599888 None:intergenic
GTGTTGGTAGGAGGAGAATT+TGG 0.206656 8_3:+57600722 None:intergenic
CAGCAAATGGCTTTCTGATT+TGG 0.214444 8_3:-57601152 Msa1297380:intron
ATATATTAAACATATCTTCT+AGG 0.256352 8_3:+57598571 None:intergenic
TTTCACATGGACTAATTAAT+AGG 0.262725 8_3:-57598490 Msa1297380:three_prime_UTR
CAGATATTATGAGTTAATAT+TGG 0.263931 8_3:-57598452 Msa1297380:three_prime_UTR
TAAAGAGCGTTTGGAAGTTT+TGG 0.276908 8_3:-57600098 Msa1297380:CDS
GACATGAATGTTTCTCATTT+GGG 0.281293 8_3:+57600625 None:intergenic
AGACATGAATGTTTCTCATT+TGG 0.284212 8_3:+57600624 None:intergenic
CATTAGCATCAAGGAAGTTT+AGG 0.295193 8_3:+57601002 None:intergenic
AAGTTTAGGTTGCATTCTTC+AGG 0.308908 8_3:+57601016 None:intergenic
GATGAATAAGTAAAATGTTC+GGG 0.311408 8_3:+57598390 None:intergenic
CCAGAAGCACAAGGGGATTC+TGG 0.313026 8_3:-57599888 Msa1297380:CDS
TGTTGGTAGGAGGAGAATTT+GGG 0.316269 8_3:+57600723 None:intergenic
TCTTGTTCCTAACCTGAAAA+AGG 0.316557 8_3:-57600450 Msa1297380:intron
GTTTCTTCTGATACGTTCAT+TGG 0.319465 8_3:+57601175 None:intergenic
TTTCAAGCTTTGTGCTGAAT+TGG 0.333352 8_3:+57601288 None:intergenic
CAGGAGTGAGTCCACAAAAC+TGG 0.341350 8_3:+57600696 None:intergenic
AGATATTATGAGTTAATATT+GGG 0.344583 8_3:-57598451 Msa1297380:three_prime_UTR
ATAGCAGTGTCTTACCCTTT+GGG 0.356671 8_3:-57599820 Msa1297380:CDS
CAAATCAGAAAGCCATTTGC+TGG 0.362893 8_3:+57601153 None:intergenic
GAAGTGTTGTTCCTTCAAAC+AGG 0.367624 8_3:+57601236 None:intergenic
GGCGTTGGCTCGAGACCTTT+TGG 0.371700 8_3:+57600541 None:intergenic
GATGATGATGGAGAAAGTTT+TGG 0.378456 8_3:+57600788 None:intergenic
AATAGCAGTGTCTTACCCTT+TGG 0.385303 8_3:-57599821 Msa1297380:CDS
GCTTTCTTTCAGCCATGATA+TGG 0.385864 8_3:+57600513 None:intergenic
CTGAACTCAAACATTGTTGT+TGG 0.389224 8_3:+57600951 None:intergenic
TCTCACTCTTCCCTGTTTGA+AGG 0.394980 8_3:-57601247 Msa1297380:five_prime_UTR
TAGAAAGGGTTTGCATTCTC+AGG 0.397241 8_3:+57598637 None:intergenic
ATCACATGCTATTTATAGAA+AGG 0.399582 8_3:+57598622 None:intergenic
GAAAGAGCTTAAAGAGCGTT+TGG 0.403263 8_3:-57600107 Msa1297380:CDS
CTGCAAGAGCAATGAAGCTT+TGG 0.405262 8_3:+57600474 None:intergenic
CATCATCATCAATGATACTA+TGG 0.407350 8_3:+57600020 None:intergenic
TCATTACACAAGTGTCATAA+TGG 0.412838 8_3:-57601215 Msa1297380:exon
AGATGAATAAGTAAAATGTT+CGG 0.413907 8_3:+57598389 None:intergenic
GTCTCAACTCAAAATCCTAA+AGG 0.422120 8_3:-57600605 Msa1297380:CDS
GTTGGTGATAGTTGTGGAAT+TGG 0.425475 8_3:+57600925 None:intergenic
TGAACTCAAACATTGTTGTT+GGG 0.428381 8_3:+57600952 None:intergenic
CAGGGAAAGAGATGCTGATT+CGG 0.434047 8_3:-57599919 Msa1297380:CDS
CAAGTGCAAGCAAAAGTGTC+AGG 0.441082 8_3:-57599938 Msa1297380:CDS
AGGAGTGAGTCCACAAAACT+GGG 0.451243 8_3:+57600697 None:intergenic
TCACATGCTATTTATAGAAA+GGG 0.453099 8_3:+57598623 None:intergenic
TCTCAACTCAAAATCCTAAA+GGG 0.458505 8_3:-57600604 Msa1297380:CDS
TCATATGATGAGAGTATTGA+AGG 0.466240 8_3:-57599980 Msa1297380:CDS
TGTTGTTGGGTTTGTTCTTG+TGG 0.480560 8_3:+57600965 None:intergenic
TATATTAAACATATCTTCTA+GGG 0.487380 8_3:+57598572 None:intergenic
TGACATTACTATCATTGCTC+AGG 0.494765 8_3:-57599789 Msa1297380:intron
AGCAATGATAGTAATGTCAA+TGG 0.495292 8_3:+57599793 None:intergenic
GATATGTTTAATATATATTG+AGG 0.495611 8_3:-57598564 Msa1297380:three_prime_UTR
TTTGACTTTGAGTTTAAGGC+AGG 0.501893 8_3:+57600677 None:intergenic
TTCAGCCATGATATGGTCAT+CGG 0.521311 8_3:+57600520 None:intergenic
CATGATATGGTCATCGGCGT+TGG 0.524246 8_3:+57600526 None:intergenic
AAACTTCCTTGATGCTAATG+AGG 0.524526 8_3:-57600999 Msa1297380:CDS
AAGATCTTTGTTATATGCAG+AGG 0.540594 8_3:-57598426 Msa1297380:three_prime_UTR
TCATCAATGATACTATGGTC+TGG 0.549545 8_3:+57600025 None:intergenic
ACTCTTCCTCATTAGCATCA+AGG 0.553454 8_3:+57600993 None:intergenic
TATCAGAAGAAACCAGCAAA+TGG 0.554188 8_3:-57601165 Msa1297380:CDS
TGAATGTTTCTCATTTGGGG+TGG 0.558418 8_3:+57600629 None:intergenic
CAACGCCGATGACCATATCA+TGG 0.570050 8_3:-57600525 Msa1297380:CDS
AAAGATGAGTTAGATGATGA+TGG 0.577216 8_3:+57600776 None:intergenic
TAGCAGTGTCTTACCCTTTG+GGG 0.577291 8_3:-57599819 Msa1297380:CDS
AGGAATATGTTCATTTCACA+TGG 0.578269 8_3:-57598503 Msa1297380:three_prime_UTR
TAAAATTTGGTAGATGGACA+AGG 0.590351 8_3:-57600161 Msa1297380:intron
ATGTCAATGGAATCCCCAAA+GGG 0.596532 8_3:+57599806 None:intergenic
AGGGTACAACTTGAGCATAA+AGG 0.600355 8_3:-57598810 Msa1297380:CDS
ACTTCAATGCTGCCATGCGT+AGG 0.601033 8_3:+57598774 None:intergenic
TGAAATTGAGAGACCAACCA+TGG 0.603140 8_3:-57600858 Msa1297380:CDS
TGGTAGGAGGAGAATTTGGG+AGG 0.603292 8_3:+57600726 None:intergenic
GAATGAGTTGGTGATAGTTG+TGG 0.603767 8_3:+57600919 None:intergenic
GGGGATTCTGGTGAAAGTCA+TGG 0.606251 8_3:-57599876 Msa1297380:CDS
GATTCACTGCCAGAAGCACA+AGG 0.606463 8_3:-57599897 Msa1297380:CDS
TTCACTGCCAGAAGCACAAG+GGG 0.606586 8_3:-57599895 Msa1297380:CDS
CATCAAAGCATGAATGAACA+AGG 0.610331 8_3:-57598745 Msa1297380:exon
AATGTCAATGGAATCCCCAA+AGG 0.614554 8_3:+57599805 None:intergenic
TGTTTCATCAGTGAATGAGT+TGG 0.619624 8_3:+57600907 None:intergenic
ACATGAATGTTTCTCATTTG+GGG 0.622328 8_3:+57600626 None:intergenic
AAGTGTTGTTCCTTCAAACA+GGG 0.627695 8_3:+57601237 None:intergenic
AAGTGCAAGCAAAAGTGTCA+GGG 0.633121 8_3:-57599937 Msa1297380:CDS
GAAAACAAAGGTAGAGTCCG+TGG 0.640082 8_3:-57600062 Msa1297380:CDS
GTCACTCTAGTTGAGATCGT+AGG 0.648592 8_3:-57598523 Msa1297380:three_prime_UTR
GAGTGAGTCCACAAAACTGG+GGG 0.649560 8_3:+57600699 None:intergenic
ATGAATAAGTAAAATGTTCG+GGG 0.657935 8_3:+57598391 None:intergenic
TGGTTTCTTCAGAACAACCA+CGG 0.659948 8_3:+57600045 None:intergenic
GGAGTGAGTCCACAAAACTG+GGG 0.660208 8_3:+57600698 None:intergenic
ATTCACTGCCAGAAGCACAA+GGG 0.669813 8_3:-57599896 Msa1297380:CDS
AGAACAAAACAAGAAAACAA+AGG 0.679620 8_3:-57600074 Msa1297380:CDS
ACTTGTCAAGAACCTACGCA+TGG 0.686710 8_3:-57598786 Msa1297380:CDS
AGTGAGTCCACAAAACTGGG+GGG 0.773710 8_3:+57600700 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTATTAATTATTATT+AGG - chr8_3:57600023-57600042 Msa1297380:CDS 0.0%
!! ATTATTATTAATTATTATTA+GGG - chr8_3:57600024-57600043 Msa1297380:CDS 0.0%
!! TCAAATACTGTTATAAAATT+TGG - chr8_3:57599499-57599518 Msa1297380:intron 15.0%
!! TTTAAACAATGAATATTTAG+TGG + chr8_3:57599942-57599961 None:intergenic 15.0%
!!! AAGTATTATTATTTTGTTTG+AGG - chr8_3:57599996-57600015 Msa1297380:CDS 15.0%
!!! ATTATTACATTTTTTGTCTA+GGG - chr8_3:57600233-57600252 Msa1297380:intron 15.0%
!!! ATTAAATGCACAAAATTTTA+AGG - chr8_3:57600507-57600526 Msa1297380:CDS 15.0%
!! TATATTAAACATATCTTCTA+GGG + chr8_3:57601104-57601123 None:intergenic 15.0%
!! ATATATTAAACATATCTTCT+AGG + chr8_3:57601105-57601124 None:intergenic 15.0%
!! GATATGTTTAATATATATTG+AGG - chr8_3:57601109-57601128 Msa1297380:intron 15.0%
!! AGATATTATGAGTTAATATT+GGG - chr8_3:57601222-57601241 Msa1297380:five_prime_UTR 15.0%
!!! CCATTATTTGATCTTTTTAT+AGG + chr8_3:57598360-57598379 None:intergenic 20.0%
!! CCTATAAAAAGATCAAATAA+TGG - chr8_3:57598357-57598376 Msa1297380:three_prime_UTR 20.0%
!!! TGATTTGGTAAGAAAATTAA+AGG - chr8_3:57598536-57598555 Msa1297380:three_prime_UTR 20.0%
!!! TAATTTTTTTTTGTTGTTGC+AGG - chr8_3:57598611-57598630 Msa1297380:three_prime_UTR 20.0%
!! ATATTTAGTGGCAATTATTA+AGG + chr8_3:57599930-57599949 None:intergenic 20.0%
!! AATAGTGAAAAAATGTTTAG+TGG + chr8_3:57599973-57599992 None:intergenic 20.0%
!!! AACTTGTTAAGCATAAAAAA+AGG - chr8_3:57600070-57600089 Msa1297380:CDS 20.0%
!! ATAATTAATGAAATGCTAAC+CGG + chr8_3:57600206-57600225 None:intergenic 20.0%
!!! CATTATTACATTTTTTGTCT+AGG - chr8_3:57600232-57600251 Msa1297380:intron 20.0%
!!! TTATTACATTTTTTGTCTAG+GGG - chr8_3:57600234-57600253 Msa1297380:intron 20.0%
!!! CTTGTGTTGTTTTTTTTTTT+TGG - chr8_3:57600384-57600403 Msa1297380:intron 20.0%
!!! CACTACTTTTTTTTTATATC+AGG - chr8_3:57600581-57600600 Msa1297380:CDS 20.0%
!!! TTTTCTATCTGTTTCTTTTT+AGG - chr8_3:57600731-57600750 Msa1297380:CDS 20.0%
!!! TCTTTTTAGGAATTTTGTTT+AGG - chr8_3:57600744-57600763 Msa1297380:CDS 20.0%
!!! TATTGTTATACTTTTGTAGA+TGG - chr8_3:57600836-57600855 Msa1297380:CDS 20.0%
!!! ATTGTTATACTTTTGTAGAT+GGG - chr8_3:57600837-57600856 Msa1297380:CDS 20.0%
!! CAGATATTATGAGTTAATAT+TGG - chr8_3:57601221-57601240 Msa1297380:five_prime_UTR 20.0%
!! AGATGAATAAGTAAAATGTT+CGG + chr8_3:57601287-57601306 None:intergenic 20.0%
! CTGTTATAAAATTTGGTAGA+TGG - chr8_3:57599506-57599525 Msa1297380:intron 25.0%
! AGAACAAAACAAGAAAACAA+AGG - chr8_3:57599599-57599618 Msa1297380:intron 25.0%
! TTTATGCTTAACAAGTTTCT+CGG + chr8_3:57600067-57600086 None:intergenic 25.0%
!! GTTAACGAAGTTTCTTTTTA+TGG + chr8_3:57600105-57600124 None:intergenic 25.0%
! TAATTAATGAAATGCTAACC+GGG + chr8_3:57600205-57600224 None:intergenic 25.0%
!!! ATAATTAACTTGTGCCTTAA+GGG + chr8_3:57600425-57600444 None:intergenic 25.0%
!!! AATAATTAACTTGTGCCTTA+AGG + chr8_3:57600426-57600445 None:intergenic 25.0%
! CGAGTGTAGAAATAATTTAT+TGG - chr8_3:57600446-57600465 Msa1297380:intron 25.0%
!! TTAAGGAATACTTCTTCTTT+TGG - chr8_3:57600524-57600543 Msa1297380:CDS 25.0%
!! TAAGGAATACTTCTTCTTTT+GGG - chr8_3:57600525-57600544 Msa1297380:CDS 25.0%
!! TGTTGGCTGTTATAATTAAT+TGG + chr8_3:57600640-57600659 None:intergenic 25.0%
!!! AAAGTATCGTCAATAATTGT+TGG + chr8_3:57600657-57600676 None:intergenic 25.0%
! TTATTACACGTATTCATGAA+AGG - chr8_3:57600684-57600703 Msa1297380:CDS 25.0%
! TATTACACGTATTCATGAAA+GGG - chr8_3:57600685-57600704 Msa1297380:CDS 25.0%
! AATACAATTAAACAACTCGT+GGG + chr8_3:57600809-57600828 None:intergenic 25.0%
! TAATACAATTAAACAACTCG+TGG + chr8_3:57600810-57600829 None:intergenic 25.0%
!! TCTGTGATTTTTATGTCAAA+TGG + chr8_3:57600971-57600990 None:intergenic 25.0%
!! TCACATGCTATTTATAGAAA+GGG + chr8_3:57601053-57601072 None:intergenic 25.0%
!! ATCACATGCTATTTATAGAA+AGG + chr8_3:57601054-57601073 None:intergenic 25.0%
!! TTTCACATGGACTAATTAAT+AGG - chr8_3:57601183-57601202 Msa1297380:CDS 25.0%
! ATGAATAAGTAAAATGTTCG+GGG + chr8_3:57601285-57601304 None:intergenic 25.0%
! GATGAATAAGTAAAATGTTC+GGG + chr8_3:57601286-57601305 None:intergenic 25.0%
TCATTACACAAGTGTCATAA+TGG - chr8_3:57598458-57598477 Msa1297380:three_prime_UTR 30.0%
!!! TTTTTTGTTGTTGCAGGAAA+TGG - chr8_3:57598617-57598636 Msa1297380:three_prime_UTR 30.0%
! TGAACTCAAACATTGTTGTT+GGG + chr8_3:57598724-57598743 None:intergenic 30.0%
!!! ACTTTTGACTCTTTTGACTT+TGG - chr8_3:57598789-57598808 Msa1297380:CDS 30.0%
AAAGATGAGTTAGATGATGA+TGG + chr8_3:57598900-57598919 None:intergenic 30.0%
!!! CTGTTTTGACTTTGAGTTTA+AGG + chr8_3:57599003-57599022 None:intergenic 30.0%
ACATGAATGTTTCTCATTTG+GGG + chr8_3:57599050-57599069 None:intergenic 30.0%
GACATGAATGTTTCTCATTT+GGG + chr8_3:57599051-57599070 None:intergenic 30.0%
AGACATGAATGTTTCTCATT+TGG + chr8_3:57599052-57599071 None:intergenic 30.0%
TCTCAACTCAAAATCCTAAA+GGG - chr8_3:57599069-57599088 Msa1297380:intron 30.0%
!!! TGTTGCAAAGTTTTGAGTTT+TGG + chr8_3:57599099-57599118 None:intergenic 30.0%
CAAAAACATCTCAAACCAAA+AGG - chr8_3:57599117-57599136 Msa1297380:intron 30.0%
TAAAATTTGGTAGATGGACA+AGG - chr8_3:57599512-57599531 Msa1297380:intron 30.0%
CATCATCATCAATGATACTA+TGG + chr8_3:57599656-57599675 None:intergenic 30.0%
!! TCATATGATGAGAGTATTGA+AGG - chr8_3:57599693-57599712 Msa1297380:intron 30.0%
AGCAATGATAGTAATGTCAA+TGG + chr8_3:57599883-57599902 None:intergenic 30.0%
TTAGTGGCAATTATTAAGGT+AGG + chr8_3:57599926-57599945 None:intergenic 30.0%
AATATCATTAATCAATGCCC+CGG - chr8_3:57600184-57600203 Msa1297380:intron 30.0%
AATTAATGAAATGCTAACCG+GGG + chr8_3:57600204-57600223 None:intergenic 30.0%
!!! AACATTGATTTTGAAAGCGA+GGG + chr8_3:57600281-57600300 None:intergenic 30.0%
!!! TTTTTTAACATGTGCCCTAA+GGG - chr8_3:57600548-57600567 Msa1297380:CDS 30.0%
AGGAATATGTTCATTTCACA+TGG - chr8_3:57601170-57601189 Msa1297380:CDS 30.0%
! AAGATCTTTGTTATATGCAG+AGG - chr8_3:57601247-57601266 Msa1297380:five_prime_UTR 30.0%
! TTTCAAGCTTTGTGCTGAAT+TGG + chr8_3:57598388-57598407 None:intergenic 35.0%
! AAGTGTTGTTCCTTCAAACA+GGG + chr8_3:57598439-57598458 None:intergenic 35.0%
GTTTCTTCTGATACGTTCAT+TGG + chr8_3:57598501-57598520 None:intergenic 35.0%
TATCAGAAGAAACCAGCAAA+TGG - chr8_3:57598508-57598527 Msa1297380:three_prime_UTR 35.0%
!! AAGTTTAGGTTGCATTCTTC+AGG + chr8_3:57598660-57598679 None:intergenic 35.0%
CATTAGCATCAAGGAAGTTT+AGG + chr8_3:57598674-57598693 None:intergenic 35.0%
AAACTTCCTTGATGCTAATG+AGG - chr8_3:57598674-57598693 Msa1297380:three_prime_UTR 35.0%
CTGAACTCAAACATTGTTGT+TGG + chr8_3:57598725-57598744 None:intergenic 35.0%
TGTTTCATCAGTGAATGAGT+TGG + chr8_3:57598769-57598788 None:intergenic 35.0%
! AACAATTGTTTTCAGCTCCA+TGG + chr8_3:57598835-57598854 None:intergenic 35.0%
!! TGGAGAAAGTTTTGGTGAAA+TGG + chr8_3:57598880-57598899 None:intergenic 35.0%
!!! GATGATGATGGAGAAAGTTT+TGG + chr8_3:57598888-57598907 None:intergenic 35.0%
! TTTGACTTTGAGTTTAAGGC+AGG + chr8_3:57598999-57599018 None:intergenic 35.0%
GTCTCAACTCAAAATCCTAA+AGG - chr8_3:57599068-57599087 Msa1297380:intron 35.0%
!!! TGAGTTTTGGATAACCCTTT+AGG + chr8_3:57599086-57599105 None:intergenic 35.0%
TCTTGTTCCTAACCTGAAAA+AGG - chr8_3:57599223-57599242 Msa1297380:intron 35.0%
!!! AGTGTTACCTTTTTCAGGTT+AGG + chr8_3:57599233-57599252 None:intergenic 35.0%
!! TGTGAAGTGTTACCTTTTTC+AGG + chr8_3:57599238-57599257 None:intergenic 35.0%
CACTTCACACTTATGTTTGA+AGG - chr8_3:57599249-57599268 Msa1297380:intron 35.0%
AATGTGTATTAGTGTCGTGT+TGG + chr8_3:57599304-57599323 None:intergenic 35.0%
!! TTGAGTGCTATTTAGCTAGT+GGG + chr8_3:57599434-57599453 None:intergenic 35.0%
!! TTTGAGTGCTATTTAGCTAG+TGG + chr8_3:57599435-57599454 None:intergenic 35.0%
!!! TAAAGAGCGTTTGGAAGTTT+TGG - chr8_3:57599575-57599594 Msa1297380:intron 35.0%
TCATCAATGATACTATGGTC+TGG + chr8_3:57599651-57599670 None:intergenic 35.0%
TGACATTACTATCATTGCTC+AGG - chr8_3:57599884-57599903 Msa1297380:CDS 35.0%
TACTATCATTGCTCAGGTAA+AGG - chr8_3:57599890-57599909 Msa1297380:CDS 35.0%
ATTATTAGGGTCATGCTAAC+CGG - chr8_3:57600037-57600056 Msa1297380:CDS 35.0%
!!! CAACATTGATTTTGAAAGCG+AGG + chr8_3:57600282-57600301 None:intergenic 35.0%
!! AATGTTAACTTGTGCCCTTA+AGG - chr8_3:57600408-57600427 Msa1297380:intron 35.0%
!!! GTTTTTTAACATGTGCCCTA+AGG - chr8_3:57600547-57600566 Msa1297380:CDS 35.0%
! ATACTTTTGTAGATGGGTGA+AGG - chr8_3:57600843-57600862 Msa1297380:CDS 35.0%
! TACTTTTGTAGATGGGTGAA+GGG - chr8_3:57600844-57600863 Msa1297380:CDS 35.0%
CATCAAAGCATGAATGAACA+AGG - chr8_3:57600928-57600947 Msa1297380:CDS 35.0%
!! GAAGTGTTGTTCCTTCAAAC+AGG + chr8_3:57598440-57598459 None:intergenic 40.0%
CAAATCAGAAAGCCATTTGC+TGG + chr8_3:57598523-57598542 None:intergenic 40.0%
CAGCAAATGGCTTTCTGATT+TGG - chr8_3:57598521-57598540 Msa1297380:three_prime_UTR 40.0%
ACTCTTCCTCATTAGCATCA+AGG + chr8_3:57598683-57598702 None:intergenic 40.0%
! TGTTGTTGGGTTTGTTCTTG+TGG + chr8_3:57598711-57598730 None:intergenic 40.0%
!! GTTGGTGATAGTTGTGGAAT+TGG + chr8_3:57598751-57598770 None:intergenic 40.0%
!! GAATGAGTTGGTGATAGTTG+TGG + chr8_3:57598757-57598776 None:intergenic 40.0%
TGAAATTGAGAGACCAACCA+TGG - chr8_3:57598815-57598834 Msa1297380:CDS 40.0%
! ATTGTTTTCAGCTCCATGGT+TGG + chr8_3:57598831-57598850 None:intergenic 40.0%
! TGTTGGTAGGAGGAGAATTT+GGG + chr8_3:57598953-57598972 None:intergenic 40.0%
TGAATGTTTCTCATTTGGGG+TGG + chr8_3:57599047-57599066 None:intergenic 40.0%
TTCAGCCATGATATGGTCAT+CGG + chr8_3:57599156-57599175 None:intergenic 40.0%
GCTTTCTTTCAGCCATGATA+TGG + chr8_3:57599163-57599182 None:intergenic 40.0%
TTACTGTCGATGTTGTGTCT+TGG - chr8_3:57599373-57599392 Msa1297380:intron 40.0%
!! TGAGTGCTATTTAGCTAGTG+GGG + chr8_3:57599433-57599452 None:intergenic 40.0%
!! GAAAGAGCTTAAAGAGCGTT+TGG - chr8_3:57599566-57599585 Msa1297380:intron 40.0%
TGGTTTCTTCAGAACAACCA+CGG + chr8_3:57599631-57599650 None:intergenic 40.0%
! CACTTTTGCTTGCACTTGAA+AGG + chr8_3:57599733-57599752 None:intergenic 40.0%
!! AAGTGCAAGCAAAAGTGTCA+GGG - chr8_3:57599736-57599755 Msa1297380:intron 40.0%
AATAGCAGTGTCTTACCCTT+TGG - chr8_3:57599852-57599871 Msa1297380:CDS 40.0%
ATAGCAGTGTCTTACCCTTT+GGG - chr8_3:57599853-57599872 Msa1297380:CDS 40.0%
ATGTCAATGGAATCCCCAAA+GGG + chr8_3:57599870-57599889 None:intergenic 40.0%
AATGTCAATGGAATCCCCAA+AGG + chr8_3:57599871-57599890 None:intergenic 40.0%
TAACAAGTTTCTCGGAGTAC+CGG + chr8_3:57600059-57600078 None:intergenic 40.0%
!! AGTGTTAACTTGTGCCCTTA+GGG + chr8_3:57600565-57600584 None:intergenic 40.0%
!! TAGTGTTAACTTGTGCCCTT+AGG + chr8_3:57600566-57600585 None:intergenic 40.0%
TACACGTATTCATGAAAGGG+AGG - chr8_3:57600688-57600707 Msa1297380:CDS 40.0%
ACACGTATTCATGAAAGGGA+GGG - chr8_3:57600689-57600708 Msa1297380:CDS 40.0%
AGGGTACAACTTGAGCATAA+AGG - chr8_3:57600863-57600882 Msa1297380:CDS 40.0%
TAGAAAGGGTTTGCATTCTC+AGG + chr8_3:57601039-57601058 None:intergenic 40.0%
TCTCACTCTTCCCTGTTTGA+AGG - chr8_3:57598426-57598445 Msa1297380:three_prime_UTR 45.0%
! GTGTTGGTAGGAGGAGAATT+TGG + chr8_3:57598954-57598973 None:intergenic 45.0%
AGGAGTGAGTCCACAAAACT+GGG + chr8_3:57598979-57598998 None:intergenic 45.0%
CTGCAAGAGCAATGAAGCTT+TGG + chr8_3:57599202-57599221 None:intergenic 45.0%
GAAAACAAAGGTAGAGTCCG+TGG - chr8_3:57599611-57599630 Msa1297380:intron 45.0%
!! CAAGTGCAAGCAAAAGTGTC+AGG - chr8_3:57599735-57599754 Msa1297380:intron 45.0%
! CAGGGAAAGAGATGCTGATT+CGG - chr8_3:57599754-57599773 Msa1297380:intron 45.0%
! ATTCACTGCCAGAAGCACAA+GGG - chr8_3:57599777-57599796 Msa1297380:intron 45.0%
TAGCAGTGTCTTACCCTTTG+GGG - chr8_3:57599854-57599873 Msa1297380:CDS 45.0%
GGTCATCTGAGTGACACATT+TGG + chr8_3:57600260-57600279 None:intergenic 45.0%
!! GGGAGGGCGTATTTTATTCT+AGG - chr8_3:57600705-57600724 Msa1297380:CDS 45.0%
ACTTGTCAAGAACCTACGCA+TGG - chr8_3:57600887-57600906 Msa1297380:CDS 45.0%
GTCACTCTAGTTGAGATCGT+AGG - chr8_3:57601150-57601169 Msa1297380:intron 45.0%
TGGTAGGAGGAGAATTTGGG+AGG + chr8_3:57598950-57598969 None:intergenic 50.0%
AGTGAGTCCACAAAACTGGG+GGG + chr8_3:57598976-57598995 None:intergenic 50.0%
GAGTGAGTCCACAAAACTGG+GGG + chr8_3:57598977-57598996 None:intergenic 50.0%
GGAGTGAGTCCACAAAACTG+GGG + chr8_3:57598978-57598997 None:intergenic 50.0%
CAGGAGTGAGTCCACAAAAC+TGG + chr8_3:57598980-57598999 None:intergenic 50.0%
CATGATATGGTCATCGGCGT+TGG + chr8_3:57599150-57599169 None:intergenic 50.0%
CAACGCCGATGACCATATCA+TGG - chr8_3:57599148-57599167 Msa1297380:intron 50.0%
! GATTCACTGCCAGAAGCACA+AGG - chr8_3:57599776-57599795 Msa1297380:intron 50.0%
! TTCACTGCCAGAAGCACAAG+GGG - chr8_3:57599778-57599797 Msa1297380:intron 50.0%
GGGGATTCTGGTGAAAGTCA+TGG - chr8_3:57599797-57599816 Msa1297380:CDS 50.0%
ACTTCAATGCTGCCATGCGT+AGG + chr8_3:57600902-57600921 None:intergenic 50.0%
!! CAAAACTGGGGGGTGTTGGT+AGG + chr8_3:57598966-57598985 None:intergenic 55.0%
! ACCAACACCCCCCAGTTTTG+TGG - chr8_3:57598966-57598985 Msa1297380:intron 55.0%
! TCCACAAAACTGGGGGGTGT+TGG + chr8_3:57598970-57598989 None:intergenic 55.0%
CCAGAATCCCCTTGTGCTTC+TGG + chr8_3:57599788-57599807 None:intergenic 55.0%
! CCAGAAGCACAAGGGGATTC+TGG - chr8_3:57599785-57599804 Msa1297380:intron 55.0%
!! AACTGGGGGGTGTTGGTAGG+AGG + chr8_3:57598963-57598982 None:intergenic 60.0%
!!! GGCGTTGGCTCGAGACCTTT+TGG + chr8_3:57599135-57599154 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 57598354 57601341 57598354 ID=Msa1297380;Name=Msa1297380
chr8_3 mRNA 57598354 57601341 57598354 ID=Msa1297380-mRNA-1;Parent=Msa1297380;Name=Msa1297380-mRNA-1;_AED=0.19;_eAED=0.19;_QI=123|1|1|1|1|1|4|397|382
chr8_3 exon 57598354 57598840 57598354 ID=Msa1297380-mRNA-1:exon:16522;Parent=Msa1297380-mRNA-1
chr8_3 exon 57599790 57600170 57599790 ID=Msa1297380-mRNA-1:exon:16521;Parent=Msa1297380-mRNA-1
chr8_3 exon 57600451 57601062 57600451 ID=Msa1297380-mRNA-1:exon:16520;Parent=Msa1297380-mRNA-1
chr8_3 exon 57601153 57601341 57601153 ID=Msa1297380-mRNA-1:exon:16519;Parent=Msa1297380-mRNA-1
chr8_3 five_prime_UTR 57601219 57601341 57601219 ID=Msa1297380-mRNA-1:five_prime_utr;Parent=Msa1297380-mRNA-1
chr8_3 CDS 57601153 57601218 57601153 ID=Msa1297380-mRNA-1:cds;Parent=Msa1297380-mRNA-1
chr8_3 CDS 57600451 57601062 57600451 ID=Msa1297380-mRNA-1:cds;Parent=Msa1297380-mRNA-1
chr8_3 CDS 57599790 57600170 57599790 ID=Msa1297380-mRNA-1:cds;Parent=Msa1297380-mRNA-1
chr8_3 CDS 57598751 57598840 57598751 ID=Msa1297380-mRNA-1:cds;Parent=Msa1297380-mRNA-1
chr8_3 three_prime_UTR 57598354 57598750 57598354 ID=Msa1297380-mRNA-1:three_prime_utr;Parent=Msa1297380-mRNA-1
Gene Sequence

>Msa1297380

ATGGAAGAAATCAACAACACACCAATGAACGTATCAGAAGAAACCAGCAAATGGCTTTCTGATTTGGAAATGGATGAGTATAATTTGTTTCCTGAAGAATGCAACCTAAACTTCCTTGATGCTAATGAGGAAGAGTTTCTTCCACAAGAACAAACCCAACAACAATGTTTGAGTTCAGAGTCCAATTCCACAACTATCACCAACTCATTCACTGATGAAACAACTTTTGACTCTTTTGACTTTGGTTTTGAAATTGAGAGACCAACCATGGAGCTGAAAACAATTGTTAGTGACAACAACATCATTGAAACCATTTCACCAAAACTTTCTCCATCATCATCTAACTCATCTTTGCACTCTCAGATTTTGTCTTTTGACAACCTCCCAAATTCTCCTCCTACCAACACCCCCCAGTTTTGTGGACTCACTCCTGCCTTAAACTCAAAGTCAAAACAGAACAAAACAGTGTCAGTGTTTCCACCCCAAATGAGAAACATTCATGTCTCAACTCAAAATCCTAAAGGGTTATCCAAAACTCAAAACTTTGCAACAAAAACATCTCAAACCAAAAGGTCTCGAGCCAACGCCGATGACCATATCATGGCTGAAAGAAAGCGAAGAGAGAAACTTAGCCAAAGCTTCATTGCTCTTGCAGCTCTTGTTCCTAACCTGAAAAAGATGGACAAGGCTTCTGTATTAGCTGAGTCTATCACATACGTGAAAGAGCTTAAAGAGCGTTTGGAAGTTTTGGAAGAACAAAACAAGAAAACAAAGGTAGAGTCCGTGGTTGTTCTGAAGAAACCAGACCATAGTATCATTGATGATGATGATGATGATGATAGCTCATCATATGATGAGAGTATTGAAGGTGCTACTGATTCATCCTTTCAAGTGCAAGCAAAAGTGTCAGGGAAAGAGATGCTGATTCGGATTCACTGCCAGAAGCACAAGGGGATTCTGGTGAAAGTCATGGCTGAGATTCAAAGCTTACAATTGTTTGCTGTGAATAGCAGTGTCTTACCCTTTGGGGATTCCATTGACATTACTATCATTGCTCAGATGGGTGAAGGGTACAACTTGAGCATAAAGGAACTTGTCAAGAACCTACGCATGGCAGCATTGAAGTTTATGTCATCAAAGCATGAATGA
Protein sequence

>Msa1297380

MEEINNTPMNVSEETSKWLSDLEMDEYNLFPEECNLNFLDANEEEFLPQEQTQQQCLSSESNSTTITNSFTDETTFDSFDFGFEIERPTMELKTIVSDNNIIETISPKLSPSSSNSSLHSQILSFDNLPNSPPTNTPQFCGLTPALNSKSKQNKTVSVFPPQMRNIHVSTQNPKGLSKTQNFATKTSQTKRSRANADDHIMAERKRREKLSQSFIALAALVPNLKKMDKASVLAESITYVKELKERLEVLEEQNKKTKVESVVVLKKPDHSIIDDDDDDDSSSYDESIEGATDSSFQVQAKVSGKEMLIRIHCQKHKGILVKVMAEIQSLQLFAVNSSVLPFGDSIDITIIAQMGEGYNLSIKELVKNLRMAALKFMSSKHE*