AlfalfaGEDB | Msa1300260

M. sativa cultivar zhongmu-4 / Msa1300260

KEGG: -
GO: -
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300260	sp\|Q9LEZ3\|BIM1_ARATH	46.850	508	205	20	1	488	1	463	6.75e-105	326

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300260	A0A072USK3	96.735	490	13	2	1	488	1	489	0.0	972

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TFs/TRs:

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Gene ID	Type	Classification
Msa1300260	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1260420	Msa1300260	0.811212	7.836296e-51	-8.615850e-47
Msa1300260	Msa1331110	0.804721	1.885419e-49	-8.615850e-47
Msa1300260	Msa1331170	0.807059	6.081168e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300260	MtrunA17_Chr4g0061791	96.735	490	13	2	1	488	1	489	0.0	972
Msa1300260	MtrunA17_Chr5g0420701	47.034	236	110	5	255	488	35	257	9.30e-59	197
Msa1300260	MtrunA17_Chr7g0220371	54.422	147	52	4	252	395	37	171	4.21e-38	142

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1300260	AT5G08130.5	47.059	510	204	20	1	488	1	466	3.14e-107	329
Msa1300260	AT5G08130.8	46.863	510	206	20	1	488	1	467	1.38e-106	328
Msa1300260	AT5G08130.7	47.047	508	203	20	1	488	1	462	1.60e-106	327
Msa1300260	AT5G08130.2	46.850	508	205	20	1	488	1	463	6.87e-106	326
Msa1300260	AT5G08130.4	46.850	508	205	20	1	488	1	463	6.87e-106	326
Msa1300260	AT5G08130.6	46.562	509	205	20	1	488	1	463	1.52e-104	322
Msa1300260	AT5G08130.3	46.966	379	154	13	119	488	2	342	1.14e-82	262
Msa1300260	AT5G08130.1	46.702	379	156	13	119	488	2	343	5.71e-82	260
Msa1300260	AT1G69010.1	41.132	265	118	10	226	486	8	238	1.27e-40	148
Msa1300260	AT5G38860.2	44.828	174	64	5	253	403	21	185	7.30e-34	127

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Find 203 sgRNAs with CRISPR-Local

Find 480 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAGCTTGTATCAGTAT+GGG	0.573845	8_3:+61752569	None:intergenic
AAAGAGCTCACCATTGAAGG+AGG	0.629369	8_3:-61753955	Msa1300260:CDS
AAATGATAAATGTTGTAATT+AGG	0.289965	8_3:-61753264	Msa1300260:three_prime_UTR
AAATTATCTCTGAATTCTGA+AGG	0.565839	8_3:+61760259	None:intergenic
AAATTTGGTTGGGAAGAAAT+AGG	0.321463	8_3:+61754120	None:intergenic
AACATTGCTGTTTGCTTCAA+AGG	0.352246	8_3:-61754277	Msa1300260:CDS
AACTCAACAGAGAAAGTTAA+AGG	0.261850	8_3:-61761065	Msa1300260:five_prime_UTR
AAGACAATAGATCATCCGTC+TGG	0.528087	8_3:-61754168	Msa1300260:CDS
AAGATCCTTCTAAGAATCAA+AGG	0.539351	8_3:-61752901	Msa1300260:three_prime_UTR
AAGCTCTTGATTTGCGATTA+AGG	0.234916	8_3:-61760418	Msa1300260:five_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACTCAACAGAGAAAGTTAA+AGG	-	chr8_3:61752401-61752420	Msa1300260:three_prime_UTR	30.0%
ACTCAACAGAGAAAGTTAAA+GGG	-	chr8_3:61752402-61752421	Msa1300260:three_prime_UTR	30.0%
AGTGATTATAAGAAAAAGCG+CGG	-	chr8_3:61752495-61752514	Msa1300260:three_prime_UTR	30.0%
GAAACCGTAATTCTCTTATT+AGG	+	chr8_3:61753138-61753157	None:intergenic	30.0%
TCTATTTAATCACAGGATCT+GGG	-	chr8_3:61753800-61753819	Msa1300260:three_prime_UTR	30.0%
CCATTTGATGTTAAATCCTT+CGG	+	chr8_3:61753903-61753922	None:intergenic	30.0%
AACTACAAGTCCATCAAAAT+GGG	+	chr8_3:61753992-61754011	None:intergenic	30.0%
AATGAGGAGAATGAAAAAAG+TGG	+	chr8_3:61754658-61754677	None:intergenic	30.0%
TGACATGTAAGTGCATTAAA+TGG	+	chr8_3:61755250-61755269	None:intergenic	30.0%
GATTTGTGCTCAAATTCTAT+GGG	+	chr8_3:61756025-61756044	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	61752327	61761161	61752327	ID=Msa1300260;Name=Msa1300260
chr8_3	mRNA	61752327	61761161	61752327	ID=Msa1300260-mRNA-1;Parent=Msa1300260;Name=Msa1300260-mRNA-1;_AED=0.04;_eAED=0.04;_QI=747\|0.90\|0.83\|1\|0.72\|0.58\|12\|1464\|488
chr8_3	exon	61752327	61752930	61752327	ID=Msa1300260-mRNA-1:exon:18279;Parent=Msa1300260-mRNA-1
chr8_3	exon	61753058	61754367	61753058	ID=Msa1300260-mRNA-1:exon:18278;Parent=Msa1300260-mRNA-1
chr8_3	exon	61754726	61754821	61754726	ID=Msa1300260-mRNA-1:exon:18277;Parent=Msa1300260-mRNA-1
chr8_3	exon	61754902	61754971	61754902	ID=Msa1300260-mRNA-1:exon:18276;Parent=Msa1300260-mRNA-1
chr8_3	exon	61755486	61755594	61755486	ID=Msa1300260-mRNA-1:exon:18275;Parent=Msa1300260-mRNA-1
chr8_3	exon	61756055	61756182	61756055	ID=Msa1300260-mRNA-1:exon:18274;Parent=Msa1300260-mRNA-1
chr8_3	exon	61756304	61756403	61756304	ID=Msa1300260-mRNA-1:exon:18273;Parent=Msa1300260-mRNA-1
chr8_3	exon	61757617	61757791	61757617	ID=Msa1300260-mRNA-1:exon:18272;Parent=Msa1300260-mRNA-1
chr8_3	exon	61757801	61758018	61757801	ID=Msa1300260-mRNA-1:exon:18271;Parent=Msa1300260-mRNA-1
chr8_3	exon	61759915	61759998	61759915	ID=Msa1300260-mRNA-1:exon:18270;Parent=Msa1300260-mRNA-1
chr8_3	exon	61760119	61760594	61760119	ID=Msa1300260-mRNA-1:exon:18269;Parent=Msa1300260-mRNA-1
chr8_3	exon	61760854	61761161	61760854	ID=Msa1300260-mRNA-1:exon:18268;Parent=Msa1300260-mRNA-1
chr8_3	five_prime_UTR	61760854	61761161	61760854	ID=Msa1300260-mRNA-1:five_prime_utr;Parent=Msa1300260-mRNA-1
chr8_3	five_prime_UTR	61760156	61760594	61760156	ID=Msa1300260-mRNA-1:five_prime_utr;Parent=Msa1300260-mRNA-1
chr8_3	CDS	61760119	61760155	61760119	ID=Msa1300260-mRNA-1:cds;Parent=Msa1300260-mRNA-1
chr8_3	CDS	61759915	61759998	61759915	ID=Msa1300260-mRNA-1:cds;Parent=Msa1300260-mRNA-1
chr8_3	CDS	61757801	61758018	61757801	ID=Msa1300260-mRNA-1:cds;Parent=Msa1300260-mRNA-1
chr8_3	CDS	61757617	61757791	61757617	ID=Msa1300260-mRNA-1:cds;Parent=Msa1300260-mRNA-1
chr8_3	CDS	61756304	61756403	61756304	ID=Msa1300260-mRNA-1:cds;Parent=Msa1300260-mRNA-1
chr8_3	CDS	61756055	61756182	61756055	ID=Msa1300260-mRNA-1:cds;Parent=Msa1300260-mRNA-1
chr8_3	CDS	61755486	61755594	61755486	ID=Msa1300260-mRNA-1:cds;Parent=Msa1300260-mRNA-1
chr8_3	CDS	61754902	61754971	61754902	ID=Msa1300260-mRNA-1:cds;Parent=Msa1300260-mRNA-1
chr8_3	CDS	61754726	61754821	61754726	ID=Msa1300260-mRNA-1:cds;Parent=Msa1300260-mRNA-1
chr8_3	CDS	61753918	61754367	61753918	ID=Msa1300260-mRNA-1:cds;Parent=Msa1300260-mRNA-1
chr8_3	three_prime_UTR	61753058	61753917	61753058	ID=Msa1300260-mRNA-1:three_prime_utr;Parent=Msa1300260-mRNA-1
chr8_3	three_prime_UTR	61752327	61752930	61752327	ID=Msa1300260-mRNA-1:three_prime_utr;Parent=Msa1300260-mRNA-1

Gene Sequence

>Msa1300260

ATGGAGCTTCCTCAAGCACGTTCTTATGGAGCCGAAGAAGCAGGGAGGAAATCGACGCATGATTTTCTGTCGCTATACAGTAATTCAACTGCCGAACAAGATCCAAGGCCATCTGCTCAAGCAAGTTTCCTCAAGACCCATGATTTCTTGCGGCCATTAGAGGAGGTAGAAGCCAAATCCAGTGCCAAGGAAGAAGCCGCAGATGAAATCTCATCGGTGGTTGAGAAGCAACCCCCTTTAGCAACACCAGCTTCATCAGTTGAGCACCTCTTACCTGGAGGAATTGGGACTTATAGTATCAGCCACATTAATTCCTGCGTGAATAATAATAATCAAAGGCCAGAAGCATCATCACTTTTCACTGTGCATCAAGCAACTAGTGCGGATAGAAATGATGACAACTCCAACTGCAGCTCACATACTTCAAGCGGTTTCACATTGTGGGAAGAGTCTGAGATTAGAAAGGGAAAGACAGGAAAGGAGAATAATGTTGGGGACAAACTCATTATAGGAGATTCTGCTGCTAAGTTAGGTCAGTGGACATTGAAGGAGCGAACGTCTCAATCGTTTTCTAACAATCATCATGGCAGCTTCAACTCTCGCTCTTCATCTCAGACAACTGGACAGAAGAACCAGAGTTTTATGGAAATGATGAAATCTGCACAGGATTGTGCCCAGGATGAAGAGCTAGAAAATGAAGGAACGTTTTTTCTCAAGAGAGAATCCTCGAATGTTCAAAGAGGAGAACTAAGGGTGAGAGTGGACGGCAAGAGTACCGATCAAAAGCCAAACACACCAAGATCAAAACATTCTGCAACAGAGCAGCGAAGAAGAAGCAAAATTAATGACAGGTTTCAGATGCTACGAGAGCTTATTCCACACAGTGATCAAAAGAGGGACAAAGCATCCTTTTTGTTAGAGGTCATTGAGTACATTCACTTTTTACAAGAGAAGGTTCATAAGTATGAAGGTTCATTCCAAGGTTGGAATAACGAGCCAGAAAAACTGATGCCATGGAGAAATAATGACAGGCCAGCAGAAAGTTTTCAATCTCGTGGCGCAAATGGTGGGTCTAATCCATCTCCAACATTGCTGTTTGCTTCAAAGGTTGACGAGAAAAACATCACCATCTCCCCAACAACTCATGGGGGTATCCATAATGTAGAATCTGGCTTGAGCACAACAACGGCCATGAAGACAATAGATCATCCGTCTGGAATAACAAACAAGGCTTTTCCAAGTCCTATTTCTTCCCAACCAAATTTCTTAACTTCAACCCAAATCGGAGGTTTAGGTGGAGCAGTGTCTCAGCTCAGACGTAGATTATCATCTGATGCAGAGAACACCAATTATCAACCTTCAGTAGAATCTCAGACTATGACTTCAACCAGTGAAAAGCTGAAAGAGAAAGAGCTCACCATTGAAGGAGGTGCCATTAGCATATCAAGTGTGTACTCACAAGGGTGA

Protein sequence

>Msa1300260

MELPQARSYGAEEAGRKSTHDFLSLYSNSTAEQDPRPSAQASFLKTHDFLRPLEEVEAKSSAKEEAADEISSVVEKQPPLATPASSVEHLLPGGIGTYSISHINSCVNNNNQRPEASSLFTVHQATSADRNDDNSNCSSHTSSGFTLWEESEIRKGKTGKENNVGDKLIIGDSAAKLGQWTLKERTSQSFSNNHHGSFNSRSSSQTTGQKNQSFMEMMKSAQDCAQDEELENEGTFFLKRESSNVQRGELRVRVDGKSTDQKPNTPRSKHSATEQRRRSKINDRFQMLRELIPHSDQKRDKASFLLEVIEYIHFLQEKVHKYEGSFQGWNNEPEKLMPWRNNDRPAESFQSRGANGGSNPSPTLLFASKVDEKNITISPTTHGGIHNVESGLSTTTAMKTIDHPSGITNKAFPSPISSQPNFLTSTQIGGLGGAVSQLRRRLSSDAENTNYQPSVESQTMTSTSEKLKEKELTIEGGAISISSVYSQG*