AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1309010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1309010 MtrunA17_Chr8g0335471 100.000 203 0 0 1 203 1 203 2.57e-153 422
Msa1309010 MtrunA17_Chr7g0250531 94.581 203 10 1 1 203 1 202 4.03e-144 399
Msa1309010 MtrunA17_Chr4g0055651 88.177 203 23 1 1 203 1 202 7.22e-135 376
Msa1309010 MtrunA17_Chr2g0276931 75.253 198 49 0 4 201 5 202 2.92e-112 318
Msa1309010 MtrunA17_Chr4g0030911 60.680 206 70 4 4 201 11 213 6.47e-85 250
Msa1309010 MtrunA17_Chr1g0212521 59.223 206 74 3 4 201 11 214 4.11e-84 248
Msa1309010 MtrunA17_Chr1g0182321 59.809 209 72 4 4 203 11 216 8.27e-84 247
Msa1309010 MtrunA17_Chr7g0263071 59.809 209 72 4 4 203 11 216 2.88e-83 246
Msa1309010 MtrunA17_Chr1g0182301 59.330 209 73 4 4 203 11 216 3.50e-83 246
Msa1309010 MtrunA17_Chr2g0327911 53.171 205 84 4 5 201 12 212 2.82e-71 215
Msa1309010 MtrunA17_Chr4g0032201 47.393 211 103 2 1 203 5 215 8.65e-67 204
Msa1309010 MtrunA17_Chr3g0112681 47.642 212 102 3 1 203 5 216 1.30e-63 196
Msa1309010 MtrunA17_Chr2g0276941 47.867 211 98 3 4 203 11 220 1.21e-62 194
Msa1309010 MtrunA17_Chr6g0458081 47.393 211 96 3 5 202 3 211 5.98e-61 189
Msa1309010 MtrunA17_Chr2g0283131 46.698 212 102 2 2 203 7 217 5.07e-60 187
Msa1309010 MtrunA17_Chr2g0315551 48.095 210 98 3 4 203 9 217 7.92e-60 186
Msa1309010 MtrunA17_Chr3g0123821 44.811 212 105 2 2 201 10 221 1.81e-59 186
Msa1309010 MtrunA17_Chr4g0040781 46.226 212 103 2 2 203 7 217 3.09e-59 185
Msa1309010 MtrunA17_Chr4g0046261 45.755 212 104 2 2 203 7 217 3.09e-59 185
Msa1309010 MtrunA17_Chr2g0290891 46.411 209 101 2 5 202 3 211 4.42e-59 184
Msa1309010 MtrunA17_Chr7g0265001 48.020 202 101 3 5 202 13 214 5.01e-59 184
Msa1309010 MtrunA17_Chr8g0381841 46.047 215 103 4 2 203 11 225 3.19e-58 182
Msa1309010 MtrunA17_Chr8g0384311 48.259 201 100 4 4 201 9 208 3.37e-58 182
Msa1309010 MtrunA17_Chr1g0159481 50.246 203 96 4 4 202 13 214 5.31e-58 181
Msa1309010 MtrunA17_Chr7g0231131 44.076 211 107 2 2 201 9 219 2.08e-57 180
Msa1309010 MtrunA17_Chr2g0325091 46.919 211 104 2 1 203 4 214 4.15e-57 179
Msa1309010 MtrunA17_Chr8g0349711 51.534 163 79 0 7 169 5 167 1.84e-56 177
Msa1309010 MtrunA17_Chr2g0319951 45.972 211 102 3 4 203 9 218 2.46e-56 177
Msa1309010 MtrunA17_Chr1g0189361 44.811 212 102 3 6 202 12 223 2.67e-56 177
Msa1309010 MtrunA17_Chr8g0351861 42.523 214 106 3 6 202 14 227 2.53e-55 175
Msa1309010 MtrunA17_Chr8g0346421 44.221 199 110 1 1 199 19 216 2.65e-55 175
Msa1309010 MtrunA17_Chr7g0234201 45.631 206 100 2 7 202 12 215 3.37e-55 174
Msa1309010 MtrunA17_Chr3g0130271 47.291 203 100 6 4 201 8 208 2.24e-54 172
Msa1309010 MtrunA17_Chr1g0174281 42.791 215 107 4 2 201 9 222 6.70e-54 171
Msa1309010 MtrunA17_Chr7g0275991 43.878 196 107 2 6 199 18 212 1.96e-53 170
Msa1309010 MtrunA17_Chr6g0482721 48.588 177 91 0 1 177 5 181 2.71e-53 169
Msa1309010 MtrunA17_Chr8g0349681 45.918 196 97 1 5 200 381 567 2.73e-53 179
Msa1309010 MtrunA17_Chr8g0349681 48.214 168 87 0 2 169 169 336 7.20e-51 172
Msa1309010 MtrunA17_Chr8g0349681 48.765 162 83 0 7 168 12 173 1.03e-48 167
Msa1309010 MtrunA17_Chr8g0392931 45.283 212 106 2 1 202 7 218 1.73e-52 167
Msa1309010 MtrunA17_Chr1g0180291 46.269 201 103 4 5 201 11 210 2.84e-52 167
Msa1309010 MtrunA17_Chr1g0197331 45.588 204 108 3 2 202 9 212 1.09e-51 165
Msa1309010 MtrunA17_Chr7g0214241 51.282 156 76 0 7 162 14 169 2.99e-50 162
Msa1309010 MtrunA17_Chr3g0109721 41.026 195 107 3 10 202 12 200 1.89e-46 152
Msa1309010 MtrunA17_Chr5g0433561 40.000 195 109 3 10 202 12 200 1.09e-44 147
Msa1309010 MtrunA17_Chr8g0390561 39.487 195 109 3 10 202 11 198 1.05e-43 144
Msa1309010 MtrunA17_Chr5g0400131 37.563 197 117 2 9 200 10 205 2.64e-42 141
Msa1309010 MtrunA17_Chr1g0199331 43.038 158 89 1 10 166 33 190 3.53e-42 140
Msa1309010 MtrunA17_Chr8g0382221 37.313 201 115 3 9 202 10 206 5.16e-42 140
Msa1309010 MtrunA17_Chr4g0055081 39.130 161 98 0 9 169 10 170 2.00e-40 136
Msa1309010 MtrunA17_Chr3g0123971 40.994 161 95 0 9 169 10 170 2.28e-40 136
Msa1309010 MtrunA17_Chr2g0331691 37.313 201 116 4 8 201 10 207 3.31e-39 133
Msa1309010 MtrunA17_Chr4g0004661 37.313 201 116 4 8 201 64 261 3.01e-38 132
Msa1309010 MtrunA17_Chr4g0034871 37.000 200 116 4 8 200 8 204 4.78e-38 130
Msa1309010 MtrunA17_Chr6g0466301 38.554 166 95 3 8 166 8 173 1.30e-34 121
Msa1309010 MtrunA17_Chr6g0465491 38.554 166 95 3 8 166 8 173 1.30e-34 121
Msa1309010 MtrunA17_Chr8g0383261 39.157 166 94 3 8 166 12 177 1.67e-34 121
Msa1309010 MtrunA17_Chr1g0201121 37.952 166 96 3 8 166 8 173 8.67e-34 119
Msa1309010 MtrunA17_Chr3g0117931 31.217 189 115 6 8 183 8 194 1.76e-23 93.2
Msa1309010 MtrunA17_Chr6g0465471 33.929 168 101 4 25 185 7 171 4.37e-23 92.0
Msa1309010 MtrunA17_Chr6g0486041 32.335 167 99 5 8 162 6 170 5.57e-23 91.7
Msa1309010 MtrunA17_Chr3g0138101 32.447 188 111 6 9 189 14 192 5.95e-23 92.0
Msa1309010 MtrunA17_Chr3g0138121 32.447 188 111 6 9 189 14 192 6.40e-23 92.0
Msa1309010 MtrunA17_Chr3g0138111 32.447 188 111 6 9 189 14 192 6.61e-23 91.7
Msa1309010 MtrunA17_Chr6g0466161 35.570 149 89 3 25 166 7 155 9.75e-23 90.9
Msa1309010 MtrunA17_Chr4g0037021 31.737 167 100 5 8 162 6 170 1.60e-22 90.1
Msa1309010 MtrunA17_Chr8g0371501 33.728 169 98 6 8 164 6 172 2.16e-22 89.7
Msa1309010 MtrunA17_Chr2g0325101 32.000 175 102 6 8 170 6 175 3.25e-22 89.4
Msa1309010 MtrunA17_Chr5g0405791 33.136 169 99 6 8 164 6 172 1.17e-21 87.8
Msa1309010 MtrunA17_Chr4g0046211 36.134 119 73 3 8 125 6 122 2.66e-21 85.5
Msa1309010 MtrunA17_Chr1g0178291 35.260 173 102 4 5 177 428 590 2.57e-20 88.2
Msa1309010 MtrunA17_Chr6g0466431 35.354 99 55 1 8 106 8 97 5.43e-17 73.2
Msa1309010 MtrunA17_Chr6g0465611 35.354 99 55 1 8 106 8 97 5.43e-17 73.2
Msa1309010 MtrunA17_Chr5g0434561 32.500 160 90 6 9 159 20 170 8.06e-14 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1309010 AT1G02130.1 91.133 203 18 0 1 203 1 203 1.60e-142 395
Msa1309010 AT5G47200.1 86.700 203 26 1 1 203 1 202 1.27e-132 370
Msa1309010 AT4G17530.1 86.207 203 27 1 1 203 1 202 8.66e-132 368
Msa1309010 AT3G11730.1 73.399 203 52 2 1 201 1 203 1.25e-110 315
Msa1309010 AT5G59840.1 60.287 209 71 4 4 203 11 216 7.58e-85 250
Msa1309010 AT3G46060.2 58.852 209 74 4 4 203 11 216 5.22e-83 245
Msa1309010 AT3G46060.3 58.852 209 74 4 4 203 11 216 5.22e-83 245
Msa1309010 AT3G46060.1 58.852 209 74 4 4 203 11 216 5.22e-83 245
Msa1309010 AT3G53610.2 58.173 208 77 3 4 203 11 216 5.34e-83 245
Msa1309010 AT3G53610.3 58.173 208 77 3 4 203 11 216 5.34e-83 245
Msa1309010 AT3G53610.1 58.173 208 77 3 4 203 11 216 5.34e-83 245
Msa1309010 AT5G03520.1 59.709 206 74 3 4 202 11 214 1.59e-80 239
Msa1309010 AT3G09900.1 58.654 208 75 3 4 202 11 216 5.24e-79 235
Msa1309010 AT1G07410.1 45.714 210 107 2 1 203 5 214 7.53e-64 197
Msa1309010 AT1G09630.1 45.498 211 106 2 2 203 6 216 3.07e-61 190
Msa1309010 AT3G46830.1 45.070 213 107 1 1 203 5 217 8.83e-61 189
Msa1309010 AT3G46830.2 45.070 213 107 1 1 203 5 217 8.83e-61 189
Msa1309010 AT4G35860.1 53.939 165 76 0 5 169 3 167 9.01e-61 189
Msa1309010 AT4G17160.1 47.317 205 97 3 5 201 3 204 1.47e-60 188
Msa1309010 AT5G45750.1 47.596 208 101 1 4 203 9 216 1.59e-60 188
Msa1309010 AT4G39990.1 47.368 209 99 3 6 203 15 223 1.85e-60 188
Msa1309010 AT4G18430.1 49.282 209 95 3 4 202 9 216 2.60e-60 188
Msa1309010 AT3G15060.1 47.170 212 101 2 2 203 7 217 4.38e-60 187
Msa1309010 AT5G03520.2 55.689 167 65 3 43 202 40 204 4.76e-60 187
Msa1309010 AT5G60860.1 46.698 212 102 2 2 203 7 217 8.14e-60 187
Msa1309010 AT2G33870.1 46.445 211 101 3 4 203 9 218 1.55e-59 186
Msa1309010 AT1G16920.1 48.077 208 100 2 4 203 9 216 1.73e-59 186
Msa1309010 AT4G17170.1 52.663 169 80 0 5 173 3 171 2.50e-59 185
Msa1309010 AT5G59150.1 43.192 213 111 1 1 203 5 217 4.31e-59 185
Msa1309010 AT5G47520.1 46.667 210 100 2 6 203 12 221 7.37e-59 184
Msa1309010 AT1G06400.1 45.238 210 107 1 2 203 7 216 1.14e-58 184
Msa1309010 AT5G47960.1 44.651 215 107 3 1 203 8 222 1.75e-58 183
Msa1309010 AT1G28550.1 45.972 211 102 3 4 203 9 218 3.19e-58 182
Msa1309010 AT4G18800.1 46.602 206 104 1 4 203 9 214 5.13e-58 182
Msa1309010 AT5G65270.1 44.811 212 103 3 6 203 15 226 5.46e-58 182
Msa1309010 AT1G73640.1 48.515 202 96 2 4 197 9 210 1.02e-57 182
Msa1309010 AT3G12160.1 44.811 212 104 3 2 201 9 219 2.78e-57 180
Msa1309010 AT3G07410.1 45.894 207 102 2 6 202 10 216 1.47e-56 178
Msa1309010 AT1G43890.3 54.118 170 75 3 2 169 7 175 5.75e-55 174
Msa1309010 AT1G43890.1 54.118 170 75 3 2 169 7 175 5.75e-55 174
Msa1309010 AT1G43890.2 54.118 170 75 3 2 169 7 175 5.75e-55 174
Msa1309010 AT2G43130.1 43.137 204 109 2 6 202 10 213 6.31e-55 174
Msa1309010 AT1G18200.1 50.000 180 90 0 2 181 7 186 1.12e-54 174
Msa1309010 AT5G03530.2 47.030 202 102 4 5 202 10 210 3.56e-53 169
Msa1309010 AT5G03530.1 47.030 202 102 4 5 202 10 210 3.56e-53 169
Msa1309010 AT1G05810.1 43.269 208 108 2 6 203 10 217 3.98e-53 169
Msa1309010 AT2G31680.1 50.955 157 77 0 6 162 10 166 7.95e-53 169
Msa1309010 AT1G05810.2 43.269 208 108 2 6 203 56 263 9.41e-53 170
Msa1309010 AT1G01200.1 43.284 201 112 2 1 199 21 221 2.40e-49 160
Msa1309010 AT4G19640.1 41.837 196 104 3 10 202 12 200 5.12e-46 151
Msa1309010 AT3G09910.3 44.335 203 102 5 5 203 10 205 9.87e-46 150
Msa1309010 AT3G09910.1 44.335 203 102 5 5 203 10 205 9.87e-46 150
Msa1309010 AT3G09910.2 44.335 203 102 5 5 203 10 205 9.87e-46 150
Msa1309010 AT5G45130.1 40.306 196 107 2 10 202 12 200 2.00e-44 147
Msa1309010 AT3G09910.5 47.953 171 86 3 5 173 10 179 3.58e-44 146
Msa1309010 AT3G09910.4 47.953 171 86 3 5 173 10 179 3.58e-44 146
Msa1309010 AT5G45130.2 40.415 193 107 3 12 202 22 208 1.10e-42 142
Msa1309010 AT3G54840.1 44.805 154 84 1 10 162 35 188 2.33e-42 142
Msa1309010 AT2G44610.1 37.073 205 111 2 9 202 10 207 2.95e-42 141
Msa1309010 AT3G54840.2 44.805 154 84 1 10 162 35 188 3.17e-42 141
Msa1309010 AT3G54840.3 44.444 153 84 1 11 162 2 154 6.87e-42 139
Msa1309010 AT4G39890.1 41.358 162 94 1 9 169 10 171 1.06e-41 140
Msa1309010 AT2G22290.1 36.500 200 118 2 9 202 10 206 4.63e-41 138
Msa1309010 AT4G35860.2 52.500 120 57 0 50 169 2 121 1.94e-40 135
Msa1309010 AT5G10260.1 40.994 161 95 0 9 169 10 170 4.94e-40 136
Msa1309010 AT1G52280.1 36.715 207 121 4 1 200 1 204 7.32e-40 135
Msa1309010 AT3G16100.1 37.500 200 115 4 8 200 8 204 8.06e-40 135
Msa1309010 AT5G59150.2 40.244 164 88 1 50 203 3 166 1.97e-39 133
Msa1309010 AT3G18820.1 38.500 200 113 4 8 200 8 204 1.28e-38 132
Msa1309010 AT5G64990.1 39.759 166 100 0 9 174 8 173 2.22e-38 131
Msa1309010 AT2G21880.1 39.759 166 93 3 8 166 9 174 1.99e-36 127
Msa1309010 AT1G49300.2 36.000 200 118 4 8 200 8 204 2.55e-36 126
Msa1309010 AT1G49300.1 36.000 200 118 4 8 200 8 204 2.55e-36 126
Msa1309010 AT4G19640.3 45.038 131 72 0 10 140 12 142 1.32e-35 124
Msa1309010 AT5G64990.2 38.150 173 100 1 9 174 8 180 1.33e-35 124
Msa1309010 AT4G19640.2 45.600 125 68 0 10 134 12 136 7.52e-35 120
Msa1309010 AT4G09720.1 40.361 166 92 3 8 166 8 173 9.01e-34 120
Msa1309010 AT1G22740.1 38.182 165 95 3 8 165 8 172 3.71e-33 118
Msa1309010 AT4G09720.4 38.012 171 94 3 8 166 8 178 1.15e-31 114
Msa1309010 AT4G09720.3 36.723 177 94 3 8 166 8 184 1.08e-30 112
Msa1309010 AT5G39620.1 38.922 167 91 3 9 166 6 170 2.14e-30 111
Msa1309010 AT2G21880.4 40.876 137 74 3 37 166 13 149 1.71e-28 105
Msa1309010 AT2G21880.3 40.876 137 74 3 37 166 13 149 1.71e-28 105
Msa1309010 AT2G21880.2 34.940 166 93 4 8 166 9 166 1.30e-26 101
Msa1309010 AT4G09720.6 41.606 137 73 3 37 166 3 139 4.33e-26 99.4
Msa1309010 AT4G09720.5 41.606 137 73 3 37 166 3 139 4.33e-26 99.4
Msa1309010 AT4G09720.2 41.606 137 73 3 37 166 3 139 4.33e-26 99.4
Msa1309010 AT2G44690.1 33.533 167 97 5 8 162 18 182 1.16e-24 96.7
Msa1309010 AT4G35020.1 35.429 175 96 7 8 170 6 175 2.75e-24 95.1
Msa1309010 AT4G35020.2 35.429 175 96 7 8 170 6 175 2.75e-24 95.1
Msa1309010 AT4G35020.3 35.429 175 96 7 8 170 6 175 2.75e-24 95.1
Msa1309010 AT3G51300.1 35.329 167 94 6 10 164 8 172 5.84e-24 94.4
Msa1309010 AT2G17800.2 34.911 169 96 6 8 164 6 172 7.45e-24 94.0
Msa1309010 AT2G17800.1 34.911 169 96 6 8 164 6 172 7.45e-24 94.0
Msa1309010 AT4G35950.1 34.320 169 97 6 8 164 6 172 2.34e-23 92.8
Msa1309010 AT4G28950.1 31.034 203 118 8 8 200 6 196 4.93e-23 92.0
Msa1309010 AT1G75840.1 32.571 175 101 6 8 170 6 175 6.47e-23 91.7
Msa1309010 AT5G55080.1 30.270 185 114 4 9 189 14 187 3.94e-22 90.1
Msa1309010 AT5G45970.1 32.335 167 99 6 8 162 6 170 4.15e-22 89.7
Msa1309010 AT5G55190.1 32.609 184 116 5 9 189 14 192 5.58e-22 89.7
Msa1309010 AT5G46025.1 42.991 107 60 1 70 175 6 112 5.72e-22 86.7
Msa1309010 AT3G48040.1 31.250 176 106 6 8 170 8 181 8.80e-22 89.0
Msa1309010 AT5G20010.1 32.065 184 117 5 9 189 14 192 1.44e-21 88.6
Msa1309010 AT5G20020.1 31.522 184 118 5 9 189 14 192 1.70e-21 88.6
Msa1309010 AT1G20090.1 31.429 175 103 6 8 170 5 174 3.04e-21 87.0
Msa1309010 AT5G62880.1 30.539 167 102 5 8 162 8 172 1.05e-19 83.6
Msa1309010 AT3G48040.2 31.013 158 95 5 8 153 8 163 2.04e-19 82.0
Msa1309010 AT4G35020.4 35.965 114 70 3 10 122 8 119 2.54e-18 78.2
Msa1309010 AT4G35020.5 35.965 114 70 3 10 122 8 119 2.54e-18 78.2
Msa1309010 AT5G62880.2 33.333 123 79 3 8 129 8 128 2.80e-18 79.3
Msa1309010 AT3G51290.2 35.211 142 78 6 35 164 634 773 1.75e-14 71.6
Msa1309010 AT5G37680.1 31.288 163 94 6 9 162 20 173 1.40e-12 63.9
Msa1309010 AT5G67560.1 32.051 156 96 5 9 159 20 170 2.01e-11 60.8
Msa1309010 AT3G49870.2 32.919 161 88 7 9 159 1 151 3.12e-11 59.7
Msa1309010 AT2G15310.1 29.286 140 82 6 10 145 19 145 6.38e-11 59.7
Msa1309010 AT3G49870.1 31.677 161 90 7 9 159 20 170 6.73e-11 59.3
Msa1309010 AT5G64813.1 22.335 197 115 5 10 170 24 218 7.06e-11 60.8
Msa1309010 AT5G64813.3 22.335 197 115 5 10 170 24 218 7.06e-11 60.8
Msa1309010 AT5G64813.2 22.335 197 115 5 10 170 24 218 7.06e-11 60.8
Msa1309010 AT3G22950.1 30.159 126 80 4 1 125 12 130 8.72e-11 58.9
Msa1309010 AT3G22950.2 30.159 126 80 4 1 125 12 130 8.72e-11 58.9

Find 92 sgRNAs with CRISPR-Local

Find 291 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTTCAAGCTCCTTCTTAT+TGG 0.139580 8_4:-5403888 Msa1309010:CDS
TTTGCACTTGTTTCCATAAA+AGG 0.153571 8_4:+5402154 None:intergenic
ATTCTTTACATACACTTTAA+TGG 0.166971 8_4:-5401130 Msa1309010:three_prime_UTR
ATACTAGTACGAACTATAAA+AGG 0.180590 8_4:-5400644 Msa1309010:three_prime_UTR
CCACCGTGTGGTTTGGGAAT+TGG 0.282481 8_4:-5401169 Msa1309010:three_prime_UTR
TGTCTTGAACTTGCTGTTCA+TGG 0.297587 8_4:-5400982 Msa1309010:three_prime_UTR
CTGCCGGGCAAGAACGATTT+AGG 0.303257 8_4:-5402625 Msa1309010:CDS
AAGTAAAATACTTTCTAGTT+AGG 0.325796 8_4:+5401259 None:intergenic
CATAATCCACCGTGTGGTTT+GGG 0.328282 8_4:-5401175 Msa1309010:three_prime_UTR
GAAATATCCTATAAATAAAA+AGG 0.344545 8_4:-5400592 Msa1309010:three_prime_UTR
GCATAATCCACCGTGTGGTT+TGG 0.344796 8_4:-5401176 Msa1309010:three_prime_UTR
TGATAATGTTAACAAGCTTT+TGG 0.346717 8_4:-5402395 Msa1309010:CDS
ACGTATTATTATGAATGATT+TGG 0.371731 8_4:+5405263 None:intergenic
CCGGTGGTACACGTGGAAAC+TGG 0.371788 8_4:+5405121 None:intergenic
CACGGTGGATTATGCAATAT+TGG 0.373350 8_4:+5401184 None:intergenic
ACAACAAATCTTACATGAAA+AGG 0.383088 8_4:+5401221 None:intergenic
CTCCTTCTTATTGGAGACTC+TGG 0.406221 8_4:-5403879 Msa1309010:CDS
TCCACTTGTAATGTTATTAG+TGG 0.409787 8_4:+5400521 None:intergenic
ATTAAAGTGTATGTAAAGAA+TGG 0.415581 8_4:+5401132 None:intergenic
AAATGTGGAAGGAGCATTCA+TGG 0.416001 8_4:-5402122 Msa1309010:CDS
CTTCTTCTTCTCTTCTCCGT+TGG 0.416764 8_4:-5405153 Msa1309010:five_prime_UTR
GTTGAATTAAAGCTTTAATA+CGG 0.428306 8_4:+5405238 None:intergenic
TTGACAGCACACTCGCTTGA+AGG 0.438444 8_4:-5400403 Msa1309010:three_prime_UTR
CCTCCAACAGTGCAGATTAG+AGG 0.441575 8_4:-5401317 Msa1309010:CDS
GTACACGTGGAAACTGGAAT+TGG 0.441991 8_4:+5405127 None:intergenic
CCTCTAATCTGCACTGTTGG+AGG 0.450000 8_4:+5401317 None:intergenic
CTTATTGGAGACTCTGGTGT+TGG 0.464289 8_4:-5403873 Msa1309010:CDS
TAATGAGCACCTATCCTTGA+TGG 0.471028 8_4:+5402087 None:intergenic
TTCTTGTAGTGGGACACTGC+CGG 0.479251 8_4:-5402641 Msa1309010:intron
TTAAAGTGTATGTAAAGAAT+GGG 0.487470 8_4:+5401133 None:intergenic
AGCTACTATCGTGGGGCACA+TGG 0.488301 8_4:-5402590 Msa1309010:CDS
ATCCTTGATGGCAGCAGCCA+TGG 0.490402 8_4:+5402099 None:intergenic
TATTGCATAATTTATTGTGA+TGG 0.491182 8_4:+5400794 None:intergenic
CAGGAATTTGCAGATCAAAT+CGG 0.492691 8_4:-5402181 Msa1309010:CDS
CAACAAATCTTACATGAAAA+GGG 0.494564 8_4:+5401222 None:intergenic
TTCGAACTGTTGAGCAGGAT+GGG 0.495201 8_4:-5402779 Msa1309010:CDS
AAGCTTGTTAACATTATCAC+TGG 0.500079 8_4:+5402399 None:intergenic
GATTTGTTGTCACTAAATAG+TGG 0.501449 8_4:-5401208 Msa1309010:three_prime_UTR
TGTCCTCTAATCTGCACTGT+TGG 0.502752 8_4:+5401314 None:intergenic
TGGAGGCCTTGCATTGTTTG+CGG 0.503248 8_4:+5401334 None:intergenic
GGCCTTGCATTGTTTGCGGA+TGG 0.508567 8_4:+5401338 None:intergenic
TACACGTGGAAACTGGAATT+GGG 0.508797 8_4:+5405128 None:intergenic
GGCCATGGCTGCTGCCATCA+AGG 0.509743 8_4:-5402101 Msa1309010:CDS
TCTTGTAGTGGGACACTGCC+GGG 0.511489 8_4:-5402640 Msa1309010:intron
GGAGGAAGAGTGATCGGATC+CGG 0.513337 8_4:+5405102 None:intergenic
TTGCATTGTTTGCGGATGGT+TGG 0.514800 8_4:+5401342 None:intergenic
CCCACTAATAACATTACAAG+TGG 0.514876 8_4:-5400522 Msa1309010:three_prime_UTR
TTACAGCAAATCTTAGAAGA+AGG 0.520470 8_4:+5403841 None:intergenic
ATGTATACATGAAGTTCGTA+TGG 0.523531 8_4:+5400928 None:intergenic
ATTCGAACTGTTGAGCAGGA+TGG 0.524930 8_4:-5402780 Msa1309010:CDS
GAAAATTCGAACTGTTGAGC+AGG 0.528077 8_4:-5402784 Msa1309010:CDS
CCAGTTTCCACGTGTACCAC+CGG 0.530828 8_4:-5405121 Msa1309010:five_prime_UTR
ATTATTGAAGCTCTCTTCAT+CGG 0.536063 8_4:+5402456 None:intergenic
GATAGCTACATAAGCACCAT+TGG 0.541145 8_4:-5402907 Msa1309010:CDS
TTAACACAAGTACTATACTT+AGG 0.541410 8_4:+5401096 None:intergenic
TACTAGTACGAACTATAAAA+GGG 0.543996 8_4:-5400643 Msa1309010:three_prime_UTR
CACCAGAGTCTCCAATAAGA+AGG 0.544091 8_4:+5403877 None:intergenic
AACCATCCGCAAACAATGCA+AGG 0.546517 8_4:-5401340 Msa1309010:CDS
TGGCTGCTGCCATCAAGGAT+AGG 0.548752 8_4:-5402096 Msa1309010:intron
CAACGGAGAAGAGAAGAAGA+AGG 0.550308 8_4:+5405154 None:intergenic
GAATAAATAAACAGTAGGTA+AGG 0.550705 8_4:+5400550 None:intergenic
TGTCCTAAATCGTTCTTGCC+CGG 0.552890 8_4:+5402622 None:intergenic
AAGTGTGGAGGAAGAGTGAT+CGG 0.569806 8_4:+5405096 None:intergenic
TGCATAGTCATAAGATTACA+TGG 0.570505 8_4:+5400850 None:intergenic
TAACCAGTAGCTACTATCGT+GGG 0.574293 8_4:-5402598 Msa1309010:CDS
CCACTTGTAATGTTATTAGT+GGG 0.579205 8_4:+5400522 None:intergenic
TAACAGAATAAATAAACAGT+AGG 0.593884 8_4:+5400545 None:intergenic
TGCAAAGGATGCTACAAATG+TGG 0.595953 8_4:-5402137 Msa1309010:CDS
CATGGCACGAATACATGAAG+TGG 0.600329 8_4:+5400868 None:intergenic
TTCAGGATTCATGACGGAGA+AGG 0.601819 8_4:+5405071 None:intergenic
TGAATAATAACGAAGGCTAA+CGG 0.608196 8_4:+5400453 None:intergenic
AAACTGGAATTGGGCACCAA+CGG 0.608236 8_4:+5405137 None:intergenic
ACGGAGAAGGTGAAGTGTGG+AGG 0.609394 8_4:+5405084 None:intergenic
ATCGGATCCGGTGGTACACG+TGG 0.609682 8_4:+5405114 None:intergenic
AAGGATGCTACAAATGTGGA+AGG 0.613051 8_4:-5402133 Msa1309010:CDS
ACTCACAAAATCAACTCCAA+TGG 0.624945 8_4:+5402891 None:intergenic
GGAAGGAGCATTCATGGCCA+TGG 0.628916 8_4:-5402116 Msa1309010:CDS
ATAACCAGTAGCTACTATCG+TGG 0.642124 8_4:-5402599 Msa1309010:CDS
TTCATGGCAAGTAAATAACA+AGG 0.642338 8_4:-5400966 Msa1309010:three_prime_UTR
ATGACGGAGAAGGTGAAGTG+TGG 0.648130 8_4:+5405081 None:intergenic
GTTGAATTGAATAATAACGA+AGG 0.650989 8_4:+5400446 None:intergenic
GCTTCAATAATGTGAAGCAG+TGG 0.652698 8_4:-5402444 Msa1309010:CDS
GGAAGAGTGATCGGATCCGG+TGG 0.657938 8_4:+5405105 None:intergenic
AAACCAATTCCCAAACCACA+CGG 0.659429 8_4:+5401166 None:intergenic
CAGATTAGAGGACAACCAGT+TGG 0.660940 8_4:-5401305 Msa1309010:CDS
AAGTGAATTTGAATCTAACG+TGG 0.689711 8_4:+5400489 None:intergenic
AACCAGTAGCTACTATCGTG+GGG 0.702592 8_4:-5402597 Msa1309010:CDS
TAACATTATCACTGGCATAG+CGG 0.705715 8_4:+5402407 None:intergenic
CCAATTCCCAAACCACACGG+TGG 0.708851 8_4:+5401169 None:intergenic
ATACTATTCAGGATTCATGA+CGG 0.723714 8_4:+5405065 None:intergenic
TCATGGCAAGTAAATAACAA+GGG 0.724517 8_4:-5400965 Msa1309010:three_prime_UTR
ATATTGCATAATCCACCGTG+TGG 0.726238 8_4:-5401181 Msa1309010:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTGTTGTTTTTTTAATTT+GGG - chr8_4:5400760-5400779 Msa1309010:intron 10.0%
!!! ATGTAAAAATGTTTTTTTAA+TGG - chr8_4:5401216-5401235 Msa1309010:three_prime_UTR 10.0%
!! ATGTTAAAATTAAATTGAAA+AGG - chr8_4:5401254-5401273 Msa1309010:three_prime_UTR 10.0%
!!! TCACATTTTTTTTTTTTTAT+AGG + chr8_4:5405066-5405085 None:intergenic 10.0%
!!! TTTTGTTCTTGTTTTTTAAA+AGG - chr8_4:5400709-5400728 Msa1309010:intron 15.0%
!!! TTTGTTCTTGTTTTTTAAAA+GGG - chr8_4:5400710-5400729 Msa1309010:intron 15.0%
!!! GAATTGTTGTTTTTTTAATT+TGG - chr8_4:5400759-5400778 Msa1309010:intron 15.0%
!!! ATTGTTGTTTTTTTAATTTG+GGG - chr8_4:5400761-5400780 Msa1309010:intron 15.0%
!!! ATTTTATGTTTTTCAAAACA+TGG - chr8_4:5401018-5401037 Msa1309010:three_prime_UTR 15.0%
!! TAAGATTACTGTTTAAAAAT+TGG - chr8_4:5401402-5401421 Msa1309010:intron 15.0%
!! AAAATAAAATACTGCAATAT+AGG + chr8_4:5401884-5401903 None:intergenic 15.0%
!! AAATAAATAACCAGTATAAA+AGG + chr8_4:5401968-5401987 None:intergenic 15.0%
!!! TACTAGTATAATTTTTATGA+TGG + chr8_4:5405023-5405042 None:intergenic 15.0%
!! GAAATATCCTATAAATAAAA+AGG - chr8_4:5405086-5405105 Msa1309010:five_prime_UTR 15.0%
!! ACGTATTATTATGAATGATT+TGG + chr8_4:5400418-5400437 None:intergenic 20.0%
!!! GTTGAATTAAAGCTTTAATA+CGG + chr8_4:5400443-5400462 None:intergenic 20.0%
!!! GTTTTGAATTAAAATTTGTG+TGG + chr8_4:5400476-5400495 None:intergenic 20.0%
!! CAAAGTAGAAAAAAAGAAAA+AGG + chr8_4:5400642-5400661 None:intergenic 20.0%
!!! TTTTTAATTTGGGGTTAATT+TGG - chr8_4:5400770-5400789 Msa1309010:intron 20.0%
!!! TTTTAATTTGGGGTTAATTT+GGG - chr8_4:5400771-5400790 Msa1309010:intron 20.0%
!! ACAAGAACAAAAATCATAAA+AGG + chr8_4:5400701-5400720 None:intergenic 20.0%
!!! TTTTGTTCTTGTTTTTCAAA+AGG - chr8_4:5400859-5400878 Msa1309010:three_prime_UTR 20.0%
!!! TTTGTTCTTGTTTTTCAAAA+GGG - chr8_4:5400860-5400879 Msa1309010:three_prime_UTR 20.0%
!! CAGAATAATCAAATCTATAA+TGG - chr8_4:5402193-5402212 Msa1309010:intron 20.0%
!! AATAATCAAATCTATAATGG+AGG - chr8_4:5402196-5402215 Msa1309010:intron 20.0%
!!! ATAATCAAATCTATAATGGA+GGG - chr8_4:5402197-5402216 Msa1309010:intron 20.0%
!! GTTGAATTAAATCTATGAAT+GGG - chr8_4:5402359-5402378 Msa1309010:CDS 20.0%
!! TTAACTTTATGATATACTAC+AGG - chr8_4:5402734-5402753 Msa1309010:intron 20.0%
!! AAAACATAGTAGTGTATATT+AGG + chr8_4:5403011-5403030 None:intergenic 20.0%
!!! TTTGAAATGTACTATGATTT+TGG - chr8_4:5404031-5404050 Msa1309010:intron 20.0%
!!! TTAGATTTCAATGCATATTT+TGG - chr8_4:5404134-5404153 Msa1309010:intron 20.0%
!!! TTTTGTTTAAAACTGACATA+CGG - chr8_4:5404157-5404176 Msa1309010:intron 20.0%
!!! AAGTAAAATACTTTCTAGTT+AGG + chr8_4:5404422-5404441 None:intergenic 20.0%
!!! TAACTAGAAAGTATTTTACT+TGG - chr8_4:5404421-5404440 Msa1309010:intron 20.0%
!! TTAAAGTGTATGTAAAGAAT+GGG + chr8_4:5404548-5404567 None:intergenic 20.0%
!! ATTAAAGTGTATGTAAAGAA+TGG + chr8_4:5404549-5404568 None:intergenic 20.0%
!! ATTCTTTACATACACTTTAA+TGG - chr8_4:5404548-5404567 Msa1309010:intron 20.0%
!! CAACAAAAATATAAAGAAAG+AGG - chr8_4:5404851-5404870 Msa1309010:intron 20.0%
!! TATTGCATAATTTATTGTGA+TGG + chr8_4:5404887-5404906 None:intergenic 20.0%
!!! GTTTTCTCCTTTTTATTTAT+AGG + chr8_4:5405096-5405115 None:intergenic 20.0%
!! TAACAGAATAAATAAACAGT+AGG + chr8_4:5405136-5405155 None:intergenic 20.0%
! ATGGTTTGAATCATTTCAAT+TGG - chr8_4:5401314-5401333 Msa1309010:CDS 25.0%
! TTTCAATTGGAATTTCATCA+TGG - chr8_4:5401327-5401346 Msa1309010:CDS 25.0%
! CAAAACTATGATAGTACTAT+TGG - chr8_4:5401357-5401376 Msa1309010:intron 25.0%
! GATTACTGTTTAAAAATTGG+TGG - chr8_4:5401405-5401424 Msa1309010:intron 25.0%
! AAGTGATTGTGTTTGATATT+TGG - chr8_4:5401682-5401701 Msa1309010:intron 25.0%
! AGTTTATCTCTATTGAGAAA+CGG - chr8_4:5401731-5401750 Msa1309010:intron 25.0%
! AAATAACCAGTATAAAAGGA+TGG + chr8_4:5401964-5401983 None:intergenic 25.0%
! ACATAACAACAAAATCAATG+GGG + chr8_4:5402019-5402038 None:intergenic 25.0%
! GACATAACAACAAAATCAAT+GGG + chr8_4:5402020-5402039 None:intergenic 25.0%
! AGACATAACAACAAAATCAA+TGG + chr8_4:5402021-5402040 None:intergenic 25.0%
! TTAACATTGAATGAGTGTAA+TGG - chr8_4:5402047-5402066 Msa1309010:intron 25.0%
! TAACATTGAATGAGTGTAAT+GGG - chr8_4:5402048-5402067 Msa1309010:intron 25.0%
!! AACTATCAGATCTATTTTGT+AGG - chr8_4:5402234-5402253 Msa1309010:intron 25.0%
! GGTTGAATTAAATCTATGAA+TGG - chr8_4:5402358-5402377 Msa1309010:CDS 25.0%
!! AAATCTATGAATGGGATTTT+TGG - chr8_4:5402367-5402386 Msa1309010:CDS 25.0%
! CAAGATATAGAAGATGATAA+TGG + chr8_4:5402456-5402475 None:intergenic 25.0%
! TTTGCTAAATCATAAACTCA+AGG + chr8_4:5402582-5402601 None:intergenic 25.0%
! ATGATATTATAAGTTCAGAC+TGG + chr8_4:5402861-5402880 None:intergenic 25.0%
! AGATTGTATGTATCTTAGAT+TGG - chr8_4:5402938-5402957 Msa1309010:intron 25.0%
!!! TGATAATGTTAACAAGCTTT+TGG - chr8_4:5403283-5403302 Msa1309010:intron 25.0%
! TCAGCTATTCAATAATAAAC+AGG + chr8_4:5403619-5403638 None:intergenic 25.0%
!!! TATTCTTTCTGGAAGTTTTA+TGG + chr8_4:5403702-5403721 None:intergenic 25.0%
! TAAAGTTTGGTTATTCTTTC+TGG + chr8_4:5403713-5403732 None:intergenic 25.0%
! TAACAATGTCAAGTAAAGTT+TGG + chr8_4:5403726-5403745 None:intergenic 25.0%
!! TATTGTGAACTGATTCATAA+TGG - chr8_4:5403964-5403983 Msa1309010:intron 25.0%
!!! AAACTTTTAAAATCAAGCTC+AGG + chr8_4:5403999-5404018 None:intergenic 25.0%
!! ATGTACTATGATTTTGGATT+TGG - chr8_4:5404037-5404056 Msa1309010:intron 25.0%
!! TTTGTTTAAAACTGACATAC+GGG - chr8_4:5404158-5404177 Msa1309010:intron 25.0%
! TTGTTCAGTAGTTAAAAAGT+CGG + chr8_4:5404269-5404288 None:intergenic 25.0%
! ATTCATGATGAAGTTAAACA+AGG + chr8_4:5404303-5404322 None:intergenic 25.0%
! CAACAAATCTTACATGAAAA+GGG + chr8_4:5404459-5404478 None:intergenic 25.0%
! ACAACAAATCTTACATGAAA+AGG + chr8_4:5404460-5404479 None:intergenic 25.0%
! TTAACACAAGTACTATACTT+AGG + chr8_4:5404585-5404604 None:intergenic 25.0%
! ATACTAGTACGAACTATAAA+AGG - chr8_4:5405034-5405053 Msa1309010:intron 25.0%
! TACTAGTACGAACTATAAAA+GGG - chr8_4:5405035-5405054 Msa1309010:intron 25.0%
! GAATAAATAAACAGTAGGTA+AGG + chr8_4:5405131-5405150 None:intergenic 25.0%
! GTTGAATTGAATAATAACGA+AGG + chr8_4:5405235-5405254 None:intergenic 25.0%
ATACTATTCAGGATTCATGA+CGG + chr8_4:5400616-5400635 None:intergenic 30.0%
! GAAAAAGGTACATACTATTC+AGG + chr8_4:5400627-5400646 None:intergenic 30.0%
!!! TGTTTTTTACAACCCTAGAT+TGG - chr8_4:5400955-5400974 Msa1309010:three_prime_UTR 30.0%
ACCTTGATATTGTGTCTAAT+TGG - chr8_4:5401100-5401119 Msa1309010:three_prime_UTR 30.0%
ACCAATTAGACACAATATCA+AGG + chr8_4:5401104-5401123 None:intergenic 30.0%
TTGCATGATAGAAACCTTAA+TGG - chr8_4:5401287-5401306 Msa1309010:CDS 30.0%
TAGAAACCTTAATGGAATCA+TGG - chr8_4:5401295-5401314 Msa1309010:CDS 30.0%
TTCAAACCATGATTCCATTA+AGG + chr8_4:5401304-5401323 None:intergenic 30.0%
TTACAGCAAATCTTAGAAGA+AGG + chr8_4:5401840-5401859 None:intergenic 30.0%
TATAAAAGGATGGAGAAACA+TGG + chr8_4:5401954-5401973 None:intergenic 30.0%
AACATTGAATGAGTGTAATG+GGG - chr8_4:5402049-5402068 Msa1309010:intron 30.0%
! CTATAATGGAGGGAATATAA+TGG - chr8_4:5402207-5402226 Msa1309010:intron 30.0%
AATAGAATCTACAGGTTGTA+AGG - chr8_4:5402275-5402294 Msa1309010:intron 30.0%
TGATATTTCCTCAAGATCTA+TGG + chr8_4:5402517-5402536 None:intergenic 30.0%
ATAGATCTTGAGGAAATATC+AGG - chr8_4:5402516-5402535 Msa1309010:intron 30.0%
TTTAGCAAAATCGTCTTATG+TGG - chr8_4:5402593-5402612 Msa1309010:CDS 30.0%
! TATTTTGTGCTGTGTATTTC+TGG - chr8_4:5402639-5402658 Msa1309010:intron 30.0%
!! TGATTTAGCTTGAACTCATT+TGG - chr8_4:5402825-5402844 Msa1309010:intron 30.0%
! TTTTGATCTGTTCTTGTAGT+GGG - chr8_4:5403027-5403046 Msa1309010:intron 30.0%
CCATGTTTAAACTCTTATTG+TGG + chr8_4:5403137-5403156 None:intergenic 30.0%
CCACAATAAGAGTTTAAACA+TGG - chr8_4:5403134-5403153 Msa1309010:intron 30.0%
TAGAGAATGAAAAACACAAC+AGG + chr8_4:5403166-5403185 None:intergenic 30.0%
TCTCTAATAACATGTTCCTT+TGG - chr8_4:5403180-5403199 Msa1309010:intron 30.0%
ATTATTGAAGCTCTCTTCAT+CGG + chr8_4:5403225-5403244 None:intergenic 30.0%
!! AAGCTTGTTAACATTATCAC+TGG + chr8_4:5403282-5403301 None:intergenic 30.0%
! AATGTTAACAAGCTTTTGGT+TGG - chr8_4:5403287-5403306 Msa1309010:intron 30.0%
TTTGCACTTGTTTCCATAAA+AGG + chr8_4:5403527-5403546 None:intergenic 30.0%
! TTTTATGGAAACAAGTGCAA+AGG - chr8_4:5403526-5403545 Msa1309010:intron 30.0%
!!! GTCAATTTTTTCGAAAGCTT+TGG + chr8_4:5403903-5403922 None:intergenic 30.0%
TGTTCAGTAGTTAAAAAGTC+GGG + chr8_4:5404268-5404287 None:intergenic 30.0%
ACTTCATCATGAATGTAGAA+TGG - chr8_4:5404309-5404328 Msa1309010:intron 30.0%
!! GATTTGTTGTCACTAAATAG+TGG - chr8_4:5404470-5404489 Msa1309010:intron 30.0%
TTCATGGCAAGTAAATAACA+AGG - chr8_4:5404712-5404731 Msa1309010:intron 30.0%
TCATGGCAAGTAAATAACAA+GGG - chr8_4:5404713-5404732 Msa1309010:intron 30.0%
ATGTATACATGAAGTTCGTA+TGG + chr8_4:5404753-5404772 None:intergenic 30.0%
TGCATAGTCATAAGATTACA+TGG + chr8_4:5404831-5404850 None:intergenic 30.0%
CCACTTGTAATGTTATTAGT+GGG + chr8_4:5405159-5405178 None:intergenic 30.0%
TCCACTTGTAATGTTATTAG+TGG + chr8_4:5405160-5405179 None:intergenic 30.0%
AAGTGAATTTGAATCTAACG+TGG + chr8_4:5405192-5405211 None:intergenic 30.0%
TGAATAATAACGAAGGCTAA+CGG + chr8_4:5405228-5405247 None:intergenic 30.0%
AACTAAAGCATGCATGCATA+TGG - chr8_4:5401142-5401161 Msa1309010:three_prime_UTR 35.0%
TGAACAGATAATCACTGCAA+AGG + chr8_4:5401777-5401796 None:intergenic 35.0%
! GTTTCTCCATCCTTTTATAC+TGG - chr8_4:5401955-5401974 Msa1309010:intron 35.0%
GTTAAACGTGAACGAAGAAA+AGG - chr8_4:5402108-5402127 Msa1309010:CDS 35.0%
!! TTTTGTAGGAGAAGTGTGTA+AGG - chr8_4:5402148-5402167 Msa1309010:CDS 35.0%
AAGGTAGGAATAGAATCTAC+AGG - chr8_4:5402167-5402186 Msa1309010:CDS 35.0%
TAATGGAGGGAATATAATGG+AGG - chr8_4:5402210-5402229 Msa1309010:intron 35.0%
! AATGGAGGGAATATAATGGA+GGG - chr8_4:5402211-5402230 Msa1309010:intron 35.0%
!! TTTTGTAGGAGAAGTGTGTA+AGG - chr8_4:5402148-5402167 Msa1309010:CDS 35.0%
AAGGTAGGAATAGAATCTAC+AGG - chr8_4:5402167-5402186 Msa1309010:CDS 35.0%
!! TCTATGAATGGGATTTTTGG+AGG - chr8_4:5402370-5402389 Msa1309010:CDS 35.0%
TAGTTTCTCCATAGATCTTG+AGG - chr8_4:5402506-5402525 Msa1309010:intron 35.0%
TGTATTGTGCAGCAGTATTA+TGG + chr8_4:5402701-5402720 None:intergenic 35.0%
ACTCACAAAATCAACTCCAA+TGG + chr8_4:5402790-5402809 None:intergenic 35.0%
!! GTTTTGATCTGTTCTTGTAG+TGG - chr8_4:5403026-5403045 Msa1309010:intron 35.0%
CATCATAAACAATCTGCCAA+AGG + chr8_4:5403199-5403218 None:intergenic 35.0%
TAACATTATCACTGGCATAG+CGG + chr8_4:5403274-5403293 None:intergenic 35.0%
! GAGAGAAAAAAAAGAGTGTG+AGG + chr8_4:5403472-5403491 None:intergenic 35.0%
!!! CTTTTTTTTCTCTCTATCGC+AGG - chr8_4:5403478-5403497 Msa1309010:intron 35.0%
CAGGAATTTGCAGATCAAAT+CGG - chr8_4:5403497-5403516 Msa1309010:intron 35.0%
! TCAAATCGGCATACCTTTTA+TGG - chr8_4:5403511-5403530 Msa1309010:intron 35.0%
AAGGCATGGTACATTAAACT+TGG + chr8_4:5403665-5403684 None:intergenic 35.0%
! AAAAACTTTTCTCCCCAATG+GGG + chr8_4:5403806-5403825 None:intergenic 35.0%
! CAAAAACTTTTCTCCCCAAT+GGG + chr8_4:5403807-5403826 None:intergenic 35.0%
! CATTGGGGAGAAAAGTTTTT+GGG - chr8_4:5403806-5403825 Msa1309010:intron 35.0%
! ATTGGGGAGAAAAGTTTTTG+GGG - chr8_4:5403807-5403826 Msa1309010:intron 35.0%
AAATCTGGACCATCTAATCT+CGG - chr8_4:5404185-5404204 Msa1309010:intron 35.0%
AACCAGTTGGTCAAAAAAGT+GGG - chr8_4:5404386-5404405 Msa1309010:intron 35.0%
! TTTCTTTTGCTCAGTTGCTT+TGG - chr8_4:5404666-5404685 Msa1309010:intron 35.0%
CCCACTAATAACATTACAAG+TGG - chr8_4:5405156-5405175 Msa1309010:five_prime_UTR 35.0%
TACACGTGGAAACTGGAATT+GGG + chr8_4:5400553-5400572 None:intergenic 40.0%
CACAAAGCTGATCCAATCTA+GGG + chr8_4:5400970-5400989 None:intergenic 40.0%
TCACAAAGCTGATCCAATCT+AGG + chr8_4:5400971-5400990 None:intergenic 40.0%
! GTTGCTTCTTGTTTCTCACA+AGG - chr8_4:5401175-5401194 Msa1309010:three_prime_UTR 40.0%
CTGTTCAAGCTCCTTCTTAT+TGG - chr8_4:5401790-5401809 Msa1309010:intron 40.0%
! TGTAATGGGGTTCTTGCTAT+TGG - chr8_4:5402062-5402081 Msa1309010:intron 40.0%
!! GTAATGGGGTTCTTGCTATT+GGG - chr8_4:5402063-5402082 Msa1309010:intron 40.0%
GAACGAAGAAAAGGACATGA+CGG - chr8_4:5402117-5402136 Msa1309010:CDS 40.0%
AACGAAGAAAAGGACATGAC+GGG - chr8_4:5402118-5402137 Msa1309010:CDS 40.0%
! TGACGGGACAGAAATTTTGT+AGG - chr8_4:5402134-5402153 Msa1309010:CDS 40.0%
GAATCTACAGGTTGTAAGGT+AGG - chr8_4:5402279-5402298 Msa1309010:intron 40.0%
!! TAAGGTAGGCATTGATGACA+TGG - chr8_4:5402293-5402312 Msa1309010:intron 40.0%
!! AATGGGATTTTTGGAGGCTT+AGG - chr8_4:5402376-5402395 Msa1309010:CDS 40.0%
!! ATTTTTGGAGGCTTAGGCTT+TGG - chr8_4:5402382-5402401 Msa1309010:CDS 40.0%
! GGCACTGAAGAACAAAGTTA+GGG + chr8_4:5402561-5402580 None:intergenic 40.0%
!! AGGCACTGAAGAACAAAGTT+AGG + chr8_4:5402562-5402581 None:intergenic 40.0%
!! TTGTGCTGTGTATTTCTGGA+AGG - chr8_4:5402643-5402662 Msa1309010:intron 40.0%
! GCAGTATTATGGAAGCGTAA+AGG + chr8_4:5402690-5402709 None:intergenic 40.0%
GATAGCTACATAAGCACCAT+TGG - chr8_4:5402771-5402790 Msa1309010:CDS 40.0%
GAAAATTCGAACTGTTGAGC+AGG - chr8_4:5402894-5402913 Msa1309010:intron 40.0%
ATAACCAGTAGCTACTATCG+TGG - chr8_4:5403079-5403098 Msa1309010:intron 40.0%
TAACCAGTAGCTACTATCGT+GGG - chr8_4:5403080-5403099 Msa1309010:intron 40.0%
GCTTCAATAATGTGAAGCAG+TGG - chr8_4:5403234-5403253 Msa1309010:intron 40.0%
GTCTGTGTTCGTGTTTACTT+CGG - chr8_4:5403418-5403437 Msa1309010:intron 40.0%
! AAAAAGAGTGTGAGGTGTTC+TGG + chr8_4:5403464-5403483 None:intergenic 40.0%
TGCAAAGGATGCTACAAATG+TGG - chr8_4:5403541-5403560 Msa1309010:intron 40.0%
AAGGATGCTACAAATGTGGA+AGG - chr8_4:5403545-5403564 Msa1309010:intron 40.0%
!! AAATGTGGAAGGAGCATTCA+TGG - chr8_4:5403556-5403575 Msa1309010:intron 40.0%
TAATGAGCACCTATCCTTGA+TGG + chr8_4:5403594-5403613 None:intergenic 40.0%
!! ATGTACCATGCCTTTGAAGT+AGG - chr8_4:5403671-5403690 Msa1309010:intron 40.0%
ATTGTTAGACCATCCCCAAT+GGG - chr8_4:5403739-5403758 Msa1309010:intron 40.0%
!! GGTCTCAAATTTTGAAGACC+TGG - chr8_4:5403764-5403783 Msa1309010:intron 40.0%
TTGAAGACCTGGTACCTATT+AGG - chr8_4:5403775-5403794 Msa1309010:intron 40.0%
! AAAACTTTTCTCCCCAATGG+GGG + chr8_4:5403805-5403824 None:intergenic 40.0%
! CCAAAAACTTTTCTCCCCAA+TGG + chr8_4:5403808-5403827 None:intergenic 40.0%
! CCATTGGGGAGAAAAGTTTT+TGG - chr8_4:5403805-5403824 Msa1309010:intron 40.0%
AATGGAGACACCTAACATTG+GGG + chr8_4:5403863-5403882 None:intergenic 40.0%
CAATGGAGACACCTAACATT+GGG + chr8_4:5403864-5403883 None:intergenic 40.0%
ACAATGGAGACACCTAACAT+TGG + chr8_4:5403865-5403884 None:intergenic 40.0%
AATGTTAGGTGTCTCCATTG+TGG - chr8_4:5403864-5403883 Msa1309010:CDS 40.0%
!! GAAACTAAGAGCATCCACAA+TGG + chr8_4:5403881-5403900 None:intergenic 40.0%
ATCCATACATTCATGCAGTC+AGG - chr8_4:5404077-5404096 Msa1309010:intron 40.0%
TCCATACATTCATGCAGTCA+GGG - chr8_4:5404078-5404097 Msa1309010:intron 40.0%
ATTCATGCAGACAGAGATGT+CGG + chr8_4:5404222-5404241 None:intergenic 40.0%
CAACCAGTTGGTCAAAAAAG+TGG - chr8_4:5404385-5404404 Msa1309010:intron 40.0%
!! CACGGTGGATTATGCAATAT+TGG + chr8_4:5404497-5404516 None:intergenic 40.0%
ATATTGCATAATCCACCGTG+TGG - chr8_4:5404497-5404516 Msa1309010:intron 40.0%
AAACCAATTCCCAAACCACA+CGG + chr8_4:5404515-5404534 None:intergenic 40.0%
!! TGTCTTGAACTTGCTGTTCA+TGG - chr8_4:5404696-5404715 Msa1309010:intron 40.0%
CAACGGAGAAGAGAAGAAGA+AGG + chr8_4:5400527-5400546 None:intergenic 45.0%
CTTCTTCTTCTCTTCTCCGT+TGG - chr8_4:5400525-5400544 Msa1309010:three_prime_UTR 45.0%
AAACTGGAATTGGGCACCAA+CGG + chr8_4:5400544-5400563 None:intergenic 45.0%
GTACACGTGGAAACTGGAAT+TGG + chr8_4:5400554-5400573 None:intergenic 45.0%
AAGTGTGGAGGAAGAGTGAT+CGG + chr8_4:5400585-5400604 None:intergenic 45.0%
! TTCAGGATTCATGACGGAGA+AGG + chr8_4:5400610-5400629 None:intergenic 45.0%
CTCCTTCTTATTGGAGACTC+TGG - chr8_4:5401799-5401818 Msa1309010:intron 45.0%
CACCAGAGTCTCCAATAAGA+AGG + chr8_4:5401804-5401823 None:intergenic 45.0%
CTTATTGGAGACTCTGGTGT+TGG - chr8_4:5401805-5401824 Msa1309010:intron 45.0%
GTTCACGTTTAACCGCACAT+TGG + chr8_4:5402101-5402120 None:intergenic 45.0%
! GTAGGAGAAGTGTGTAAGGT+AGG - chr8_4:5402152-5402171 Msa1309010:CDS 45.0%
! GTAGGAGAAGTGTGTAAGGT+AGG - chr8_4:5402152-5402171 Msa1309010:CDS 45.0%
! ATTGATGACATGGAGGTGGA+GGG - chr8_4:5402303-5402322 Msa1309010:intron 45.0%
TGGAGGTGGAGGGAATATAA+TGG - chr8_4:5402313-5402332 Msa1309010:intron 45.0%
AGGTGGAGGGAATATAATGG+AGG - chr8_4:5402316-5402335 Msa1309010:intron 45.0%
! GGTGGAGGGAATATAATGGA+GGG - chr8_4:5402317-5402336 Msa1309010:intron 45.0%
! GGGAATATAATGGAGGGAAC+TGG - chr8_4:5402217-5402236 Msa1309010:intron 45.0%
! ATAATGGAGGGAACTGGACT+AGG - chr8_4:5402329-5402348 Msa1309010:intron 45.0%
TAATGGAGGGAACTGGACTA+GGG - chr8_4:5402330-5402349 Msa1309010:intron 45.0%
ATTCGAACTGTTGAGCAGGA+TGG - chr8_4:5402898-5402917 Msa1309010:CDS 45.0%
TTCGAACTGTTGAGCAGGAT+GGG - chr8_4:5402899-5402918 Msa1309010:CDS 45.0%
TGTCCTAAATCGTTCTTGCC+CGG + chr8_4:5403059-5403078 None:intergenic 45.0%
AACCAGTAGCTACTATCGTG+GGG - chr8_4:5403081-5403100 Msa1309010:intron 45.0%
TATGGCTGAGCCTACTTCAA+AGG + chr8_4:5403684-5403703 None:intergenic 45.0%
CATTGTTAGACCATCCCCAA+TGG - chr8_4:5403738-5403757 Msa1309010:intron 45.0%
TTGTTAGACCATCCCCAATG+GGG - chr8_4:5403740-5403759 Msa1309010:intron 45.0%
! AAATTTGAGACCCCCCCATT+GGG + chr8_4:5403756-5403775 None:intergenic 45.0%
! AAAATTTGAGACCCCCCCAT+TGG + chr8_4:5403757-5403776 None:intergenic 45.0%
CTATTAGGTGTACCCCCATT+GGG - chr8_4:5403790-5403809 Msa1309010:intron 45.0%
TATTAGGTGTACCCCCATTG+GGG - chr8_4:5403791-5403810 Msa1309010:intron 45.0%
CACTTAAGACCCCCAATGTT+AGG - chr8_4:5403850-5403869 Msa1309010:CDS 45.0%
ATGGAGACACCTAACATTGG+GGG + chr8_4:5403862-5403881 None:intergenic 45.0%
TCCCTGACTGCATGAATGTA+TGG + chr8_4:5404082-5404101 None:intergenic 45.0%
TGACATACGGGCTTGAAATC+TGG - chr8_4:5404170-5404189 Msa1309010:intron 45.0%
ACCATCTAATCTCGGTCCAA+CGG - chr8_4:5404193-5404212 Msa1309010:intron 45.0%
TCTCTGTCTGCATGAATGAG+TGG - chr8_4:5404225-5404244 Msa1309010:intron 45.0%
TTGCATTGTTTGCGGATGGT+TGG + chr8_4:5404339-5404358 None:intergenic 45.0%
AACCATCCGCAAACAATGCA+AGG - chr8_4:5404338-5404357 Msa1309010:intron 45.0%
TGTCCTCTAATCTGCACTGT+TGG + chr8_4:5404367-5404386 None:intergenic 45.0%
CAGATTAGAGGACAACCAGT+TGG - chr8_4:5404373-5404392 Msa1309010:intron 45.0%
!!! CACCCACTTTTTTGACCAAC+TGG + chr8_4:5404391-5404410 None:intergenic 45.0%
CATAATCCACCGTGTGGTTT+GGG - chr8_4:5404503-5404522 Msa1309010:intron 45.0%
CATGGCACGAATACATGAAG+TGG + chr8_4:5404813-5404832 None:intergenic 45.0%
! ATGACGGAGAAGGTGAAGTG+TGG + chr8_4:5400600-5400619 None:intergenic 50.0%
CCTGATTAGCGTCCAATGTG+CGG - chr8_4:5402086-5402105 Msa1309010:intron 50.0%
!! GGTAGGCATTGATGACATGG+AGG - chr8_4:5402296-5402315 Msa1309010:intron 50.0%
!! AGGCATTGATGACATGGAGG+TGG - chr8_4:5402299-5402318 Msa1309010:intron 50.0%
! CATTGATGACATGGAGGTGG+AGG - chr8_4:5402302-5402321 Msa1309010:intron 50.0%
! AATGGAGGGAACTGGACTAG+GGG - chr8_4:5402331-5402350 Msa1309010:intron 50.0%
! TTCTTGTAGTGGGACACTGC+CGG - chr8_4:5403037-5403056 Msa1309010:intron 50.0%
CTGAGCCTACTTCAAAGGCA+TGG + chr8_4:5403679-5403698 None:intergenic 50.0%
TGTTAGACCATCCCCAATGG+GGG - chr8_4:5403741-5403760 Msa1309010:intron 50.0%
! AATTTGAGACCCCCCCATTG+GGG + chr8_4:5403755-5403774 None:intergenic 50.0%
GGGTACACCTAATAGGTACC+AGG + chr8_4:5403785-5403804 None:intergenic 50.0%
! CCAATGGGGGTACACCTAAT+AGG + chr8_4:5403792-5403811 None:intergenic 50.0%
CCTATTAGGTGTACCCCCAT+TGG - chr8_4:5403789-5403808 Msa1309010:intron 50.0%
TTGGGGTCTTACAAGACCCA+AGG - chr8_4:5403824-5403843 Msa1309010:intron 50.0%
GGGGTCTTAAGTGAGACCTT+GGG + chr8_4:5403843-5403862 None:intergenic 50.0%
CATGCAGTCAGGGACATTGA+CGG - chr8_4:5404088-5404107 Msa1309010:intron 50.0%
TGGAGGCCTTGCATTGTTTG+CGG + chr8_4:5404347-5404366 None:intergenic 50.0%
CCTCTAATCTGCACTGTTGG+AGG + chr8_4:5404364-5404383 None:intergenic 50.0%
CCTCCAACAGTGCAGATTAG+AGG - chr8_4:5404361-5404380 Msa1309010:intron 50.0%
GCATAATCCACCGTGTGGTT+TGG - chr8_4:5404502-5404521 Msa1309010:intron 50.0%
TTGACAGCACACTCGCTTGA+AGG - chr8_4:5405275-5405294 Msa1309010:five_prime_UTR 50.0%
CCAGTTTCCACGTGTACCAC+CGG - chr8_4:5400557-5400576 Msa1309010:three_prime_UTR 55.0%
GGAGGAAGAGTGATCGGATC+CGG + chr8_4:5400579-5400598 None:intergenic 55.0%
ACGGAGAAGGTGAAGTGTGG+AGG + chr8_4:5400597-5400616 None:intergenic 55.0%
CCGCACATTGGACGCTAATC+AGG + chr8_4:5402089-5402108 None:intergenic 55.0%
! TCTTGTAGTGGGACACTGCC+GGG - chr8_4:5403038-5403057 Msa1309010:intron 55.0%
CTGCCGGGCAAGAACGATTT+AGG - chr8_4:5403053-5403072 Msa1309010:intron 55.0%
TGCCCCACGATAGTAGCTAC+TGG + chr8_4:5403086-5403105 None:intergenic 55.0%
AGCTACTATCGTGGGGCACA+TGG - chr8_4:5403088-5403107 Msa1309010:intron 55.0%
!! GGAAGGAGCATTCATGGCCA+TGG - chr8_4:5403562-5403581 Msa1309010:intron 55.0%
! ATCCTTGATGGCAGCAGCCA+TGG + chr8_4:5403582-5403601 None:intergenic 55.0%
TGGCTGCTGCCATCAAGGAT+AGG - chr8_4:5403582-5403601 Msa1309010:intron 55.0%
GTTAGACCATCCCCAATGGG+GGG - chr8_4:5403742-5403761 Msa1309010:intron 55.0%
TTAGACCATCCCCAATGGGG+GGG - chr8_4:5403743-5403762 Msa1309010:intron 55.0%
GGGGGTCTTAAGTGAGACCT+TGG + chr8_4:5403844-5403863 None:intergenic 55.0%
TCAGGGACATTGACGGCTGT+TGG - chr8_4:5404095-5404114 Msa1309010:intron 55.0%
GCCGTTGGACCGAGATTAGA+TGG + chr8_4:5404197-5404216 None:intergenic 55.0%
CATGCAGACAGAGATGTCGG+TGG + chr8_4:5404219-5404238 None:intergenic 55.0%
GGCCTTGCATTGTTTGCGGA+TGG + chr8_4:5404343-5404362 None:intergenic 55.0%
CCAATTCCCAAACCACACGG+TGG + chr8_4:5404512-5404531 None:intergenic 55.0%
! CCACCGTGTGGTTTGGGAAT+TGG - chr8_4:5404509-5404528 Msa1309010:intron 55.0%
CCGGTGGTACACGTGGAAAC+TGG + chr8_4:5400560-5400579 None:intergenic 60.0%
ATCGGATCCGGTGGTACACG+TGG + chr8_4:5400567-5400586 None:intergenic 60.0%
GGAAGAGTGATCGGATCCGG+TGG + chr8_4:5400576-5400595 None:intergenic 60.0%
! GAGGGAACTGGACTAGGGGA+GGG - chr8_4:5402335-5402354 Msa1309010:intron 60.0%
! AGGGAACTGGACTAGGGGAG+GGG - chr8_4:5402336-5402355 Msa1309010:intron 60.0%
ACAGAGATGTCGGTGGCCGT+TGG + chr8_4:5404212-5404231 None:intergenic 60.0%
! GGAGGGAACTGGACTAGGGG+AGG - chr8_4:5402334-5402353 Msa1309010:intron 65.0%
! GGGAACTGGACTAGGGGAGG+GGG - chr8_4:5402337-5402356 Msa1309010:intron 65.0%
GGCCATGGCTGCTGCCATCA+AGG - chr8_4:5403577-5403596 Msa1309010:intron 65.0%
TGAGACCCCCCCATTGGGGA+TGG + chr8_4:5403751-5403770 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 5400392 5405308 5400392 ID=Msa1309010;Name=Msa1309010
chr8_4 mRNA 5400392 5405308 5400392 ID=Msa1309010-mRNA-1;Parent=Msa1309010;Name=Msa1309010-mRNA-1;_AED=0.09;_eAED=0.09;_QI=226|1|1|1|0.62|0.66|9|785|203
chr8_4 exon 5400392 5400685 5400392 ID=Msa1309010-mRNA-1:exon:1753;Parent=Msa1309010-mRNA-1
chr8_4 exon 5400786 5401373 5400786 ID=Msa1309010-mRNA-1:exon:1752;Parent=Msa1309010-mRNA-1
chr8_4 exon 5402097 5402200 5402097 ID=Msa1309010-mRNA-1:exon:1751;Parent=Msa1309010-mRNA-1
chr8_4 exon 5402339 5402494 5402339 ID=Msa1309010-mRNA-1:exon:1750;Parent=Msa1309010-mRNA-1
chr8_4 exon 5402583 5402654 5402583 ID=Msa1309010-mRNA-1:exon:1749;Parent=Msa1309010-mRNA-1
chr8_4 exon 5402758 5402805 5402758 ID=Msa1309010-mRNA-1:exon:1748;Parent=Msa1309010-mRNA-1
chr8_4 exon 5402897 5402944 5402897 ID=Msa1309010-mRNA-1:exon:1747;Parent=Msa1309010-mRNA-1
chr8_4 exon 5403845 5403917 5403845 ID=Msa1309010-mRNA-1:exon:1746;Parent=Msa1309010-mRNA-1
chr8_4 exon 5405069 5405308 5405069 ID=Msa1309010-mRNA-1:exon:1745;Parent=Msa1309010-mRNA-1
chr8_4 five_prime_UTR 5405083 5405308 5405083 ID=Msa1309010-mRNA-1:five_prime_utr;Parent=Msa1309010-mRNA-1
chr8_4 CDS 5405069 5405082 5405069 ID=Msa1309010-mRNA-1:cds;Parent=Msa1309010-mRNA-1
chr8_4 CDS 5403845 5403917 5403845 ID=Msa1309010-mRNA-1:cds;Parent=Msa1309010-mRNA-1
chr8_4 CDS 5402897 5402944 5402897 ID=Msa1309010-mRNA-1:cds;Parent=Msa1309010-mRNA-1
chr8_4 CDS 5402758 5402805 5402758 ID=Msa1309010-mRNA-1:cds;Parent=Msa1309010-mRNA-1
chr8_4 CDS 5402583 5402654 5402583 ID=Msa1309010-mRNA-1:cds;Parent=Msa1309010-mRNA-1
chr8_4 CDS 5402339 5402494 5402339 ID=Msa1309010-mRNA-1:cds;Parent=Msa1309010-mRNA-1
chr8_4 CDS 5402097 5402200 5402097 ID=Msa1309010-mRNA-1:cds;Parent=Msa1309010-mRNA-1
chr8_4 CDS 5401277 5401373 5401277 ID=Msa1309010-mRNA-1:cds;Parent=Msa1309010-mRNA-1
chr8_4 three_prime_UTR 5400786 5401276 5400786 ID=Msa1309010-mRNA-1:three_prime_utr;Parent=Msa1309010-mRNA-1
chr8_4 three_prime_UTR 5400392 5400685 5400392 ID=Msa1309010-mRNA-1:three_prime_utr;Parent=Msa1309010-mRNA-1
Gene Sequence

>Msa1309010

ATGAATCCTGAATATGATTATCTGTTCAAGCTCCTTCTTATTGGAGACTCTGGTGTTGGTAAATCATGCCTTCTTCTAAGATTTGCTGATGATTCATACATTGATAGCTACATAAGCACCATTGGAGTTGATTTTAAAATTCGAACTGTTGAGCAGGATGGGAAGACAATTAAGCTACAGATTTGGGACACTGCCGGGCAAGAACGATTTAGGACAATAACCAGTAGCTACTATCGTGGGGCACATGGAATCATTATTGTTTATGATGTGACCGATGAAGAGAGCTTCAATAATGTGAAGCAGTGGCTCAGTGAAATTGACCGCTATGCCAGTGATAATGTTAACAAGCTTTTGGTTGGAAACAAGAGTGATCTGACAGCAAATAGAGCTGTCTCATATGATACAGCTAAAGAATTTGCAGATCAAATCGGCATACCTTTTATGGAAACAAGTGCAAAGGATGCTACAAATGTGGAAGGAGCATTCATGGCCATGGCTGCTGCCATCAAGGATAGAATGGCGAGCCAACCATCCGCAAACAATGCAAGGCCTCCAACAGTGCAGATTAGAGGACAACCAGTTGGTCAAAAAAGTGGGTGCTGCTCTTCCTAA
Protein sequence

>Msa1309010

MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDSYIDSYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDEESFNNVKQWLSEIDRYASDNVNKLLVGNKSDLTANRAVSYDTAKEFADQIGIPFMETSAKDATNVEGAFMAMAAAIKDRMASQPSANNARPPTVQIRGQPVGQKSGCCSS*