AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1316420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1316420 MtrunA17_Chr8g0346501 100.000 106 0 0 1 106 1 106 3.26e-77 222
Msa1316420 MtrunA17_Chr3g0127211 67.619 105 34 0 1 105 1 105 1.43e-50 155
Msa1316420 MtrunA17_Chr1g0188931 63.725 102 37 0 1 102 1 102 1.13e-47 147
Msa1316420 MtrunA17_Chr5g0436971 50.505 99 49 0 8 106 12 110 1.38e-33 113
Msa1316420 MtrunA17_Chr3g0113291 52.128 94 45 0 8 101 35 128 1.86e-33 113
Msa1316420 MtrunA17_Chr4g0046851 50.000 100 49 1 3 102 8 106 8.28e-31 105
Msa1316420 MtrunA17_Chr7g0242231 48.454 97 50 0 10 106 14 110 4.40e-30 103
Msa1316420 MtrunA17_Chr2g0316921 48.421 95 49 0 8 102 12 106 7.52e-29 100
Msa1316420 MtrunA17_Chr8g0360291 44.211 95 53 0 8 102 12 106 1.91e-26 95.1
Msa1316420 MtrunA17_Chr1g0188951 64.516 62 22 0 44 105 1 62 2.60e-22 82.4
Msa1316420 MtrunA17_Chr2g0316891 50.794 63 31 0 40 102 42 104 1.40e-18 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1316420 AT4G27745.2 66.667 102 34 0 1 102 1 102 1.63e-47 147
Msa1316420 AT4G27745.3 66.667 102 34 0 1 102 1 102 1.63e-47 147
Msa1316420 AT4G27745.1 66.667 102 34 0 1 102 1 102 1.63e-47 147
Msa1316420 AT4G27745.4 66.667 102 34 0 1 102 1 102 1.63e-47 147
Msa1316420 AT3G11230.1 50.505 99 49 0 8 106 12 110 1.23e-34 115
Msa1316420 AT3G11230.2 50.505 99 49 0 8 106 45 143 4.47e-34 115
Msa1316420 AT3G11230.4 52.174 92 44 0 8 99 12 103 1.76e-33 112
Msa1316420 AT4G27740.1 51.064 94 46 0 9 102 9 102 2.71e-33 112
Msa1316420 AT3G55890.4 52.475 101 47 1 1 101 6 105 3.21e-33 112
Msa1316420 AT3G55890.1 52.475 101 47 1 1 101 6 105 3.21e-33 112
Msa1316420 AT3G11230.3 52.747 91 43 0 8 98 45 135 6.02e-33 112
Msa1316420 AT5G53940.1 50.526 95 47 0 8 102 12 106 2.25e-32 110
Msa1316420 AT2G40110.1 49.495 99 50 0 8 106 12 110 3.91e-32 109
Msa1316420 AT3G55890.3 52.577 97 45 1 1 97 6 101 4.46e-32 108
Msa1316420 AT3G55890.2 52.577 97 45 1 1 97 6 101 4.46e-32 108
Msa1316420 AT3G08990.2 50.000 102 50 1 1 102 6 106 9.04e-32 108
Msa1316420 AT3G08990.1 50.000 102 50 1 1 102 6 106 9.04e-32 108
Msa1316420 AT3G08990.3 50.000 102 50 1 1 102 6 106 9.04e-32 108
Msa1316420 AT2G40110.2 50.000 92 46 0 8 99 12 103 8.94e-31 105
Msa1316420 AT2G40110.4 45.205 73 40 0 8 80 12 84 1.29e-21 82.0
Msa1316420 AT2G40110.3 45.205 73 40 0 8 80 12 84 1.29e-21 82.0

Find 40 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGTTTCCTCATAAGCTTTA+AGG 0.102549 8_4:+16711306 None:intergenic
TGAGAAGTTTAAGATTGTTT+TGG 0.258269 8_4:-16711255 Msa1316420:CDS
AAAGTTCACCTGAAAATCTT+TGG 0.258418 8_4:+16712209 None:intergenic
ATAAGCTGTCTATCTTCTTT+AGG 0.260634 8_4:+16711395 None:intergenic
TCATGTCAGAGTTGGATAAT+TGG 0.261612 8_4:+16712478 None:intergenic
CCATTCAACAATTAACATTA+GGG 0.290608 8_4:+16711044 None:intergenic
TTTAACATTAATTGTTCATT+GGG 0.318516 8_4:-16711207 Msa1316420:three_prime_UTR
ACCATTCAACAATTAACATT+AGG 0.327093 8_4:+16711043 None:intergenic
ATCATTAATGGCCGATTTGA+TGG 0.329137 8_4:-16712295 Msa1316420:exon
AAAGAAGATTCCAAAAGAAT+TGG 0.353208 8_4:+16712582 None:intergenic
TTTGTAAATGTTGCTTGATC+AGG 0.356684 8_4:-16711140 Msa1316420:three_prime_UTR
CATGGAAAGCAACAAGGTTC+TGG 0.376925 8_4:+16712244 None:intergenic
GGAGCTAAGGTGGCAGTGTT+AGG 0.379271 8_4:-16712325 Msa1316420:five_prime_UTR
TGAGGAAACACAGAAGTATA+AGG 0.390368 8_4:-16711294 Msa1316420:CDS
CCCTAATGTTAATTGTTGAA+TGG 0.402426 8_4:-16711044 Msa1316420:three_prime_UTR
GAAATACCTTAAAGCTTATG+AGG 0.433394 8_4:-16711312 Msa1316420:CDS
CAACTCTGACATGAAAAGAT+TGG 0.449933 8_4:-16712469 Msa1316420:five_prime_UTR
CAACAAACCTTCTCTGTACA+AGG 0.478519 8_4:+16710963 None:intergenic
GCAAAGAATATGAATGAGAA+GGG 0.486362 8_4:+16712550 None:intergenic
GGCCGATTTGATGGGATCAC+GGG 0.488237 8_4:-16712286 Msa1316420:CDS
TCAGAGTTGGATAATTGGTT+TGG 0.496251 8_4:+16712483 None:intergenic
TGCCCGTGATCCCATCAAAT+CGG 0.500160 8_4:+16712284 None:intergenic
TTGTAAATGTTGCTTGATCA+GGG 0.506988 8_4:-16711139 Msa1316420:three_prime_UTR
TTATTAGCCTTGTACAGAGA+AGG 0.526516 8_4:-16710970 Msa1316420:three_prime_UTR
TCATTAATGGCCGATTTGAT+GGG 0.534012 8_4:-16712294 Msa1316420:exon
TGGCCGATTTGATGGGATCA+CGG 0.537450 8_4:-16712287 Msa1316420:CDS
TATGTTTAACAGGCAAGCAA+TGG 0.540111 8_4:-16711461 Msa1316420:intron
CTTTGGAAACAATATCATCA+TGG 0.545782 8_4:+16712226 None:intergenic
TCTGATTGTGGAGAGGAACT+TGG 0.548681 8_4:-16711338 Msa1316420:CDS
AGCAAAGAATATGAATGAGA+AGG 0.555819 8_4:+16712549 None:intergenic
GAAGATAGACAGCTTATGAC+TGG 0.563935 8_4:-16711389 Msa1316420:CDS
TTATGTATGAGTAGGAGCTA+AGG 0.582837 8_4:-16712338 Msa1316420:intron
TTATACTAATGTTGTACATG+TGG 0.589916 8_4:+16711179 None:intergenic
TGTAAATGTTGCTTGATCAG+GGG 0.602297 8_4:-16711138 Msa1316420:three_prime_UTR
GAAACACAGAAGTATAAGGA+AGG 0.612715 8_4:-16711290 Msa1316420:CDS
ATTGTGGAGAGGAACTTGGT+TGG 0.615032 8_4:-16711334 Msa1316420:CDS
TGTATGAGTAGGAGCTAAGG+TGG 0.621962 8_4:-16712335 Msa1316420:intron
TATCATCATGGAAAGCAACA+AGG 0.636612 8_4:+16712238 None:intergenic
TTAACATTAATTGTTCATTG+GGG 0.648404 8_4:-16711206 Msa1316420:three_prime_UTR
CATTCAACAATTAACATTAG+GGG 0.686208 8_4:+16711045 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATTTGTTTAAATCAAA+TGG - chr8_4:16711500-16711519 Msa1316420:intron 10.0%
!!! AATTTACAATATGATTTTTT+TGG + chr8_4:16711911-16711930 None:intergenic 10.0%
!!! TTAATTTGTTGAATATTAAT+TGG - chr8_4:16712008-16712027 Msa1316420:intron 10.0%
!!! AAAAATTTGTTTAAATCAAA+TGG - chr8_4:16711500-16711519 Msa1316420:intron 10.0%
!!! AATTTACAATATGATTTTTT+TGG + chr8_4:16711911-16711930 None:intergenic 10.0%
!!! TTAATTTGTTGAATATTAAT+TGG - chr8_4:16712008-16712027 Msa1316420:intron 10.0%
!! TATATTATTTATGTATGAGT+AGG - chr8_4:16711208-16711227 Msa1316420:three_prime_UTR 15.0%
!! TTAGGAAAAAAAATCATTAA+TGG - chr8_4:16711247-16711266 Msa1316420:CDS 15.0%
!!! AATTTTTTCTCTAAATCTAA+CGG + chr8_4:16711487-16711506 None:intergenic 15.0%
!!! TTTTAACATTAATTGTTCAT+TGG - chr8_4:16712346-16712365 Msa1316420:intron 15.0%
!! TTTAACATTAATTGTTCATT+GGG - chr8_4:16712347-16712366 Msa1316420:intron 15.0%
!! TATATTATTTATGTATGAGT+AGG - chr8_4:16711208-16711227 Msa1316420:three_prime_UTR 15.0%
!! TTAGGAAAAAAAATCATTAA+TGG - chr8_4:16711247-16711266 Msa1316420:CDS 15.0%
!!! AATTTTTTCTCTAAATCTAA+CGG + chr8_4:16711487-16711506 None:intergenic 15.0%
!!! TTTTAACATTAATTGTTCAT+TGG - chr8_4:16712346-16712365 Msa1316420:intron 15.0%
!! TTTAACATTAATTGTTCATT+GGG - chr8_4:16712347-16712366 Msa1316420:intron 15.0%
!! AAGAATTGGACAAAAAATAT+TGG + chr8_4:16710961-16710980 None:intergenic 20.0%
!!! TATTTTTTGTCCAATTCTTT+TGG - chr8_4:16710962-16710981 Msa1316420:three_prime_UTR 20.0%
!! AGTATATAAAAGAAAAAGCT+TGG + chr8_4:16711151-16711170 None:intergenic 20.0%
!!! ACTTTTCTTTCTTTCAATTT+TGG - chr8_4:16711363-16711382 Msa1316420:CDS 20.0%
!! TTAACATTAATTGTTCATTG+GGG - chr8_4:16712348-16712367 Msa1316420:intron 20.0%
!! AAGAATTGGACAAAAAATAT+TGG + chr8_4:16710961-16710980 None:intergenic 20.0%
!!! TATTTTTTGTCCAATTCTTT+TGG - chr8_4:16710962-16710981 Msa1316420:three_prime_UTR 20.0%
!! AGTATATAAAAGAAAAAGCT+TGG + chr8_4:16711151-16711170 None:intergenic 20.0%
!!! ACTTTTCTTTCTTTCAATTT+TGG - chr8_4:16711363-16711382 Msa1316420:CDS 20.0%
!! TTAACATTAATTGTTCATTG+GGG - chr8_4:16712348-16712367 Msa1316420:intron 20.0%
! AAAGAAGATTCCAAAAGAAT+TGG + chr8_4:16710975-16710994 None:intergenic 25.0%
!! TATTGTTTCCAAAGATTTTC+AGG - chr8_4:16711337-16711356 Msa1316420:CDS 25.0%
!! TATTAGATGATCCATTTTCA+TGG + chr8_4:16711436-16711455 None:intergenic 25.0%
! AGAGAAGTGAAATTAATTTC+AGG + chr8_4:16711721-16711740 None:intergenic 25.0%
! ATTAATTTCACTTCTCTCAA+TGG - chr8_4:16711724-16711743 Msa1316420:intron 25.0%
! TTCATTTCTTCTGATCAATT+CGG + chr8_4:16711802-16711821 None:intergenic 25.0%
! AGAAGAAATGAAACAATCAT+TGG - chr8_4:16711810-16711829 Msa1316420:intron 25.0%
! TTGAATATTAATTGGACAAG+AGG - chr8_4:16712016-16712035 Msa1316420:intron 25.0%
! TGAATATTAATTGGACAAGA+GGG - chr8_4:16712017-16712036 Msa1316420:intron 25.0%
!! TTAACCTCAAAGATTTTCAA+AGG - chr8_4:16712069-16712088 Msa1316420:intron 25.0%
!! ATAACCTTTGAAAATCTTTG+AGG + chr8_4:16712076-16712095 None:intergenic 25.0%
! TTTCAAAGGTTATGTTTAAC+AGG - chr8_4:16712083-16712102 Msa1316420:intron 25.0%
!!! TGAGAAGTTTAAGATTGTTT+TGG - chr8_4:16712299-16712318 Msa1316420:exon 25.0%
!!! GAGAAGTTTAAGATTGTTTT+GGG - chr8_4:16712300-16712319 Msa1316420:exon 25.0%
!!! TTTAAGATTGTTTTGGGAAA+TGG - chr8_4:16712306-16712325 Msa1316420:exon 25.0%
!!! TTAAGATTGTTTTGGGAAAT+GGG - chr8_4:16712307-16712326 Msa1316420:exon 25.0%
! TTATACTAATGTTGTACATG+TGG + chr8_4:16712378-16712397 None:intergenic 25.0%
! CATTCAACAATTAACATTAG+GGG + chr8_4:16712512-16712531 None:intergenic 25.0%
! CCATTCAACAATTAACATTA+GGG + chr8_4:16712513-16712532 None:intergenic 25.0%
! ACCATTCAACAATTAACATT+AGG + chr8_4:16712514-16712533 None:intergenic 25.0%
! AAAGAAGATTCCAAAAGAAT+TGG + chr8_4:16710975-16710994 None:intergenic 25.0%
!! TATTGTTTCCAAAGATTTTC+AGG - chr8_4:16711337-16711356 Msa1316420:CDS 25.0%
!! TATTAGATGATCCATTTTCA+TGG + chr8_4:16711436-16711455 None:intergenic 25.0%
! AGAGAAGTGAAATTAATTTC+AGG + chr8_4:16711721-16711740 None:intergenic 25.0%
! ATTAATTTCACTTCTCTCAA+TGG - chr8_4:16711724-16711743 Msa1316420:intron 25.0%
! TTCATTTCTTCTGATCAATT+CGG + chr8_4:16711802-16711821 None:intergenic 25.0%
! AGAAGAAATGAAACAATCAT+TGG - chr8_4:16711810-16711829 Msa1316420:intron 25.0%
! TTGAATATTAATTGGACAAG+AGG - chr8_4:16712016-16712035 Msa1316420:intron 25.0%
! TGAATATTAATTGGACAAGA+GGG - chr8_4:16712017-16712036 Msa1316420:intron 25.0%
!! TTAACCTCAAAGATTTTCAA+AGG - chr8_4:16712069-16712088 Msa1316420:intron 25.0%
!! ATAACCTTTGAAAATCTTTG+AGG + chr8_4:16712076-16712095 None:intergenic 25.0%
! TTTCAAAGGTTATGTTTAAC+AGG - chr8_4:16712083-16712102 Msa1316420:intron 25.0%
!!! TGAGAAGTTTAAGATTGTTT+TGG - chr8_4:16712299-16712318 Msa1316420:exon 25.0%
!!! GAGAAGTTTAAGATTGTTTT+GGG - chr8_4:16712300-16712319 Msa1316420:exon 25.0%
!!! TTTAAGATTGTTTTGGGAAA+TGG - chr8_4:16712306-16712325 Msa1316420:exon 25.0%
!!! TTAAGATTGTTTTGGGAAAT+GGG - chr8_4:16712307-16712326 Msa1316420:exon 25.0%
! TTATACTAATGTTGTACATG+TGG + chr8_4:16712378-16712397 None:intergenic 25.0%
! CATTCAACAATTAACATTAG+GGG + chr8_4:16712512-16712531 None:intergenic 25.0%
! CCATTCAACAATTAACATTA+GGG + chr8_4:16712513-16712532 None:intergenic 25.0%
! ACCATTCAACAATTAACATT+AGG + chr8_4:16712514-16712533 None:intergenic 25.0%
GCAAAGAATATGAATGAGAA+GGG + chr8_4:16711007-16711026 None:intergenic 30.0%
AGCAAAGAATATGAATGAGA+AGG + chr8_4:16711008-16711027 None:intergenic 30.0%
! CTTTGGAAACAATATCATCA+TGG + chr8_4:16711331-16711350 None:intergenic 30.0%
AAAGTTCACCTGAAAATCTT+TGG + chr8_4:16711348-16711367 None:intergenic 30.0%
AAACGATTCATCCATGAAAA+TGG - chr8_4:16711422-16711441 Msa1316420:CDS 30.0%
AAATGGATCATCTAATACTG+TGG - chr8_4:16711439-16711458 Msa1316420:CDS 30.0%
GCTTTCATCGTGATTATATT+GGG + chr8_4:16711684-16711703 None:intergenic 30.0%
AGCTTTCATCGTGATTATAT+TGG + chr8_4:16711685-16711704 None:intergenic 30.0%
TAAAGGAAATGGACAAAATC+TGG + chr8_4:16711865-16711884 None:intergenic 30.0%
! AGATTTTGTCCATTTCCTTT+AGG - chr8_4:16711864-16711883 Msa1316420:intron 30.0%
!!! TTGGTTTTAATTTAACCCTC+TGG + chr8_4:16711892-16711911 None:intergenic 30.0%
! TCATGCCATGAACATATTTT+TGG - chr8_4:16712134-16712153 Msa1316420:intron 30.0%
! CATGCCATGAACATATTTTT+GGG - chr8_4:16712135-16712154 Msa1316420:intron 30.0%
ATAAGCTGTCTATCTTCTTT+AGG + chr8_4:16712162-16712181 None:intergenic 30.0%
!!! GATGTTTTTTGTTCTGATTG+TGG - chr8_4:16712204-16712223 Msa1316420:intron 30.0%
GAAATACCTTAAAGCTTATG+AGG - chr8_4:16712242-16712261 Msa1316420:CDS 30.0%
! TTTGTAAATGTTGCTTGATC+AGG - chr8_4:16712414-16712433 Msa1316420:intron 30.0%
! TTGTAAATGTTGCTTGATCA+GGG - chr8_4:16712415-16712434 Msa1316420:intron 30.0%
CCCTAATGTTAATTGTTGAA+TGG - chr8_4:16712510-16712529 Msa1316420:five_prime_UTR 30.0%
GCAAAGAATATGAATGAGAA+GGG + chr8_4:16711007-16711026 None:intergenic 30.0%
AGCAAAGAATATGAATGAGA+AGG + chr8_4:16711008-16711027 None:intergenic 30.0%
! CTTTGGAAACAATATCATCA+TGG + chr8_4:16711331-16711350 None:intergenic 30.0%
AAAGTTCACCTGAAAATCTT+TGG + chr8_4:16711348-16711367 None:intergenic 30.0%
AAACGATTCATCCATGAAAA+TGG - chr8_4:16711422-16711441 Msa1316420:CDS 30.0%
AAATGGATCATCTAATACTG+TGG - chr8_4:16711439-16711458 Msa1316420:CDS 30.0%
GCTTTCATCGTGATTATATT+GGG + chr8_4:16711684-16711703 None:intergenic 30.0%
AGCTTTCATCGTGATTATAT+TGG + chr8_4:16711685-16711704 None:intergenic 30.0%
TAAAGGAAATGGACAAAATC+TGG + chr8_4:16711865-16711884 None:intergenic 30.0%
! AGATTTTGTCCATTTCCTTT+AGG - chr8_4:16711864-16711883 Msa1316420:intron 30.0%
!!! TTGGTTTTAATTTAACCCTC+TGG + chr8_4:16711892-16711911 None:intergenic 30.0%
! TCATGCCATGAACATATTTT+TGG - chr8_4:16712134-16712153 Msa1316420:intron 30.0%
! CATGCCATGAACATATTTTT+GGG - chr8_4:16712135-16712154 Msa1316420:intron 30.0%
ATAAGCTGTCTATCTTCTTT+AGG + chr8_4:16712162-16712181 None:intergenic 30.0%
!!! GATGTTTTTTGTTCTGATTG+TGG - chr8_4:16712204-16712223 Msa1316420:intron 30.0%
GAAATACCTTAAAGCTTATG+AGG - chr8_4:16712242-16712261 Msa1316420:CDS 30.0%
! TTTGTAAATGTTGCTTGATC+AGG - chr8_4:16712414-16712433 Msa1316420:intron 30.0%
! TTGTAAATGTTGCTTGATCA+GGG - chr8_4:16712415-16712434 Msa1316420:intron 30.0%
CCCTAATGTTAATTGTTGAA+TGG - chr8_4:16712510-16712529 Msa1316420:five_prime_UTR 30.0%
TCAGAGTTGGATAATTGGTT+TGG + chr8_4:16711074-16711093 None:intergenic 35.0%
TCATGTCAGAGTTGGATAAT+TGG + chr8_4:16711079-16711098 None:intergenic 35.0%
! CAATCTTTTCATGTCAGAGT+TGG + chr8_4:16711087-16711106 None:intergenic 35.0%
CAACTCTGACATGAAAAGAT+TGG - chr8_4:16711085-16711104 Msa1316420:three_prime_UTR 35.0%
TTATGTATGAGTAGGAGCTA+AGG - chr8_4:16711216-16711235 Msa1316420:three_prime_UTR 35.0%
!! ATCATTAATGGCCGATTTGA+TGG - chr8_4:16711259-16711278 Msa1316420:CDS 35.0%
!! TCATTAATGGCCGATTTGAT+GGG - chr8_4:16711260-16711279 Msa1316420:CDS 35.0%
TATCATCATGGAAAGCAACA+AGG + chr8_4:16711319-16711338 None:intergenic 35.0%
! AAAATGTCTTCTAGTGGGTA+TGG + chr8_4:16711577-16711596 None:intergenic 35.0%
!!! ACCCACTAGAAGACATTTTT+TGG - chr8_4:16711578-16711597 Msa1316420:intron 35.0%
CCCAAAAAATGTCTTCTAGT+GGG + chr8_4:16711582-16711601 None:intergenic 35.0%
!!! CCCACTAGAAGACATTTTTT+GGG - chr8_4:16711579-16711598 Msa1316420:intron 35.0%
ACCCAAAAAATGTCTTCTAG+TGG + chr8_4:16711583-16711602 None:intergenic 35.0%
TGCTTAACGAGCTTCAATTA+AGG - chr8_4:16711776-16711795 Msa1316420:intron 35.0%
ATATTAATTGGACAAGAGGG+TGG - chr8_4:16712020-16712039 Msa1316420:intron 35.0%
TATGTTTAACAGGCAAGCAA+TGG - chr8_4:16712093-16712112 Msa1316420:intron 35.0%
AGGTCCCAAAAATATGTTCA+TGG + chr8_4:16712142-16712161 None:intergenic 35.0%
! TTTTTGTTCTGATTGTGGAG+AGG - chr8_4:16712209-16712228 Msa1316420:intron 35.0%
GTGTTTCCTCATAAGCTTTA+AGG + chr8_4:16712251-16712270 None:intergenic 35.0%
! TGAGGAAACACAGAAGTATA+AGG - chr8_4:16712260-16712279 Msa1316420:CDS 35.0%
! GAAACACAGAAGTATAAGGA+AGG - chr8_4:16712264-16712283 Msa1316420:CDS 35.0%
! TGTAAATGTTGCTTGATCAG+GGG - chr8_4:16712416-16712435 Msa1316420:intron 35.0%
!! TTATTAGCCTTGTACAGAGA+AGG - chr8_4:16712584-16712603 Msa1316420:five_prime_UTR 35.0%
TCAGAGTTGGATAATTGGTT+TGG + chr8_4:16711074-16711093 None:intergenic 35.0%
TCATGTCAGAGTTGGATAAT+TGG + chr8_4:16711079-16711098 None:intergenic 35.0%
! CAATCTTTTCATGTCAGAGT+TGG + chr8_4:16711087-16711106 None:intergenic 35.0%
CAACTCTGACATGAAAAGAT+TGG - chr8_4:16711085-16711104 Msa1316420:three_prime_UTR 35.0%
TTATGTATGAGTAGGAGCTA+AGG - chr8_4:16711216-16711235 Msa1316420:three_prime_UTR 35.0%
!! ATCATTAATGGCCGATTTGA+TGG - chr8_4:16711259-16711278 Msa1316420:CDS 35.0%
!! TCATTAATGGCCGATTTGAT+GGG - chr8_4:16711260-16711279 Msa1316420:CDS 35.0%
TATCATCATGGAAAGCAACA+AGG + chr8_4:16711319-16711338 None:intergenic 35.0%
! AAAATGTCTTCTAGTGGGTA+TGG + chr8_4:16711577-16711596 None:intergenic 35.0%
!!! ACCCACTAGAAGACATTTTT+TGG - chr8_4:16711578-16711597 Msa1316420:intron 35.0%
CCCAAAAAATGTCTTCTAGT+GGG + chr8_4:16711582-16711601 None:intergenic 35.0%
!!! CCCACTAGAAGACATTTTTT+GGG - chr8_4:16711579-16711598 Msa1316420:intron 35.0%
ACCCAAAAAATGTCTTCTAG+TGG + chr8_4:16711583-16711602 None:intergenic 35.0%
TGCTTAACGAGCTTCAATTA+AGG - chr8_4:16711776-16711795 Msa1316420:intron 35.0%
ATATTAATTGGACAAGAGGG+TGG - chr8_4:16712020-16712039 Msa1316420:intron 35.0%
TATGTTTAACAGGCAAGCAA+TGG - chr8_4:16712093-16712112 Msa1316420:intron 35.0%
AGGTCCCAAAAATATGTTCA+TGG + chr8_4:16712142-16712161 None:intergenic 35.0%
! TTTTTGTTCTGATTGTGGAG+AGG - chr8_4:16712209-16712228 Msa1316420:intron 35.0%
GTGTTTCCTCATAAGCTTTA+AGG + chr8_4:16712251-16712270 None:intergenic 35.0%
! TGAGGAAACACAGAAGTATA+AGG - chr8_4:16712260-16712279 Msa1316420:CDS 35.0%
! GAAACACAGAAGTATAAGGA+AGG - chr8_4:16712264-16712283 Msa1316420:CDS 35.0%
! TGTAAATGTTGCTTGATCAG+GGG - chr8_4:16712416-16712435 Msa1316420:intron 35.0%
!! TTATTAGCCTTGTACAGAGA+AGG - chr8_4:16712584-16712603 Msa1316420:five_prime_UTR 35.0%
AATCAAATGGTTCCTACCAC+AGG - chr8_4:16711513-16711532 Msa1316420:intron 40.0%
ATCAAATGGTTCCTACCACA+GGG - chr8_4:16711514-16711533 Msa1316420:intron 40.0%
TAAGTAGAAGAACATCCCTG+TGG + chr8_4:16711532-16711551 None:intergenic 40.0%
CAGGGATGTTCTTCTACTTA+TGG - chr8_4:16711532-16711551 Msa1316420:intron 40.0%
TGGGTATGGATGAACGAAAT+TGG + chr8_4:16711563-16711582 None:intergenic 40.0%
! CATTTCCTTTAGGAACCAGA+GGG - chr8_4:16711874-16711893 Msa1316420:intron 40.0%
! TTTAACCCTCTGGTTCCTAA+AGG + chr8_4:16711882-16711901 None:intergenic 40.0%
GAAGATAGACAGCTTATGAC+TGG - chr8_4:16712165-16712184 Msa1316420:intron 40.0%
CAACAAACCTTCTCTGTACA+AGG + chr8_4:16712594-16712613 None:intergenic 40.0%
AATCAAATGGTTCCTACCAC+AGG - chr8_4:16711513-16711532 Msa1316420:intron 40.0%
ATCAAATGGTTCCTACCACA+GGG - chr8_4:16711514-16711533 Msa1316420:intron 40.0%
TAAGTAGAAGAACATCCCTG+TGG + chr8_4:16711532-16711551 None:intergenic 40.0%
CAGGGATGTTCTTCTACTTA+TGG - chr8_4:16711532-16711551 Msa1316420:intron 40.0%
TGGGTATGGATGAACGAAAT+TGG + chr8_4:16711563-16711582 None:intergenic 40.0%
! CATTTCCTTTAGGAACCAGA+GGG - chr8_4:16711874-16711893 Msa1316420:intron 40.0%
! TTTAACCCTCTGGTTCCTAA+AGG + chr8_4:16711882-16711901 None:intergenic 40.0%
GAAGATAGACAGCTTATGAC+TGG - chr8_4:16712165-16712184 Msa1316420:intron 40.0%
CAACAAACCTTCTCTGTACA+AGG + chr8_4:16712594-16712613 None:intergenic 40.0%
TGTATGAGTAGGAGCTAAGG+TGG - chr8_4:16711219-16711238 Msa1316420:three_prime_UTR 45.0%
CATGGAAAGCAACAAGGTTC+TGG + chr8_4:16711313-16711332 None:intergenic 45.0%
! ACTGTGGAATAGTGTTGCTG+CGG - chr8_4:16711455-16711474 Msa1316420:intron 45.0%
TAGAAGAACATCCCTGTGGT+AGG + chr8_4:16711528-16711547 None:intergenic 45.0%
! CCTCTGGTTCCTAAAGGAAA+TGG + chr8_4:16711876-16711895 None:intergenic 45.0%
! CCATTTCCTTTAGGAACCAG+AGG - chr8_4:16711873-16711892 Msa1316420:intron 45.0%
TAATTGGACAAGAGGGTGGT+TGG - chr8_4:16712024-16712043 Msa1316420:intron 45.0%
TCTGATTGTGGAGAGGAACT+TGG - chr8_4:16712216-16712235 Msa1316420:intron 45.0%
ATTGTGGAGAGGAACTTGGT+TGG - chr8_4:16712220-16712239 Msa1316420:CDS 45.0%
TGTATGAGTAGGAGCTAAGG+TGG - chr8_4:16711219-16711238 Msa1316420:three_prime_UTR 45.0%
CATGGAAAGCAACAAGGTTC+TGG + chr8_4:16711313-16711332 None:intergenic 45.0%
! ACTGTGGAATAGTGTTGCTG+CGG - chr8_4:16711455-16711474 Msa1316420:intron 45.0%
TAGAAGAACATCCCTGTGGT+AGG + chr8_4:16711528-16711547 None:intergenic 45.0%
! CCTCTGGTTCCTAAAGGAAA+TGG + chr8_4:16711876-16711895 None:intergenic 45.0%
! CCATTTCCTTTAGGAACCAG+AGG - chr8_4:16711873-16711892 Msa1316420:intron 45.0%
TAATTGGACAAGAGGGTGGT+TGG - chr8_4:16712024-16712043 Msa1316420:intron 45.0%
TCTGATTGTGGAGAGGAACT+TGG - chr8_4:16712216-16712235 Msa1316420:intron 45.0%
ATTGTGGAGAGGAACTTGGT+TGG - chr8_4:16712220-16712239 Msa1316420:CDS 45.0%
!! TGGCCGATTTGATGGGATCA+CGG - chr8_4:16711267-16711286 Msa1316420:CDS 50.0%
TGCCCGTGATCCCATCAAAT+CGG + chr8_4:16711273-16711292 None:intergenic 50.0%
!! TGGCCGATTTGATGGGATCA+CGG - chr8_4:16711267-16711286 Msa1316420:CDS 50.0%
TGCCCGTGATCCCATCAAAT+CGG + chr8_4:16711273-16711292 None:intergenic 50.0%
GGAGCTAAGGTGGCAGTGTT+AGG - chr8_4:16711229-16711248 Msa1316420:exon 55.0%
!! GGCCGATTTGATGGGATCAC+GGG - chr8_4:16711268-16711287 Msa1316420:CDS 55.0%
GGAGCTAAGGTGGCAGTGTT+AGG - chr8_4:16711229-16711248 Msa1316420:exon 55.0%
!! GGCCGATTTGATGGGATCAC+GGG - chr8_4:16711268-16711287 Msa1316420:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 16710956 16712620 16710956 ID=Msa1316420;Name=Msa1316420
chr8_4 mRNA 16710956 16712620 16710956 ID=Msa1316420-mRNA-1;Parent=Msa1316420;Name=Msa1316420-mRNA-1;_AED=0.24;_eAED=0.24;_QI=212|1|1|1|0.5|0.33|3|288|106
chr8_4 exon 16710956 16711471 16710956 ID=Msa1316420-mRNA-1:exon:6267;Parent=Msa1316420-mRNA-1
chr8_4 exon 16712218 16712346 16712218 ID=Msa1316420-mRNA-1:exon:6266;Parent=Msa1316420-mRNA-1
chr8_4 exon 16712445 16712620 16712445 ID=Msa1316420-mRNA-1:exon:6265;Parent=Msa1316420-mRNA-1
chr8_4 five_prime_UTR 16712445 16712620 16712445 ID=Msa1316420-mRNA-1:five_prime_utr;Parent=Msa1316420-mRNA-1
chr8_4 five_prime_UTR 16712311 16712346 16712311 ID=Msa1316420-mRNA-1:five_prime_utr;Parent=Msa1316420-mRNA-1
chr8_4 CDS 16712218 16712310 16712218 ID=Msa1316420-mRNA-1:cds;Parent=Msa1316420-mRNA-1
chr8_4 CDS 16711244 16711471 16711244 ID=Msa1316420-mRNA-1:cds;Parent=Msa1316420-mRNA-1
chr8_4 three_prime_UTR 16710956 16711243 16710956 ID=Msa1316420-mRNA-1:three_prime_utr;Parent=Msa1316420-mRNA-1
Gene Sequence

>Msa1316420

ATGGCCGATTTGATGGGATCACGGGCATACTGCTGCTTCAAGTGCCAGAACCTTGTTGCTTTCCATGATGATATTGTTTCCAAAGATTTTCAGGCAAGCAATGGAAGAGCTTTTCTGTTTTCTCATGCCATGAACATATTTTTGGGACCTAAAGAAGATAGACAGCTTATGACTGGTCTTCACACAGTTGCTGATGTTTTTTGTTCTGATTGTGGAGAGGAACTTGGTTGGAAATACCTTAAAGCTTATGAGGAAACACAGAAGTATAAGGAAGGAAAATGTGTGCTTGAGAAGTTTAAGATTGTTTTGGGAAATGGGTAG
Protein sequence

>Msa1316420

MADLMGSRAYCCFKCQNLVAFHDDIVSKDFQASNGRAFLFSHAMNIFLGPKEDRQLMTGLHTVADVFCSDCGEELGWKYLKAYEETQKYKEGKCVLEKFKIVLGNG*