AlfalfaGEDB | Msa1236360

M. sativa cultivar zhongmu-4 / Msa1236360

KEGG: -
GO: -
PFAM: PF03226.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1236360	XP_024629387.1	100.000	106	0	0	1	106	1	106	3.55e-73	222

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1236360	sp\|Q9T096\|YIPL6_ARATH	66.667	102	34	0	1	102	1	102	1.60e-46	147

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0028620	Msa1236360	0.818296	2.111163e-52	-8.615850e-47
Msa0079370	Msa1236360	0.809084	2.253509e-50	-8.615850e-47
Msa0644170	Msa1236360	0.802889	4.528789e-49	-8.615850e-47
Msa0683980	Msa1236360	0.809342	1.983898e-50	-8.615850e-47
Msa0738340	Msa1236360	0.800639	1.311667e-48	-8.615850e-47
Msa0741220	Msa1236360	0.811865	5.652572e-51	-8.615850e-47
Msa0784480	Msa1236360	0.804970	1.673004e-49	-8.615850e-47
Msa0818060	Msa1236360	0.804702	1.902831e-49	-8.615850e-47
Msa0831110	Msa1236360	0.805862	1.087377e-49	-8.615850e-47
Msa0219590	Msa1236360	0.806279	8.887725e-50	-8.615850e-47
Msa1236360	Msa1277690	0.854254	1.391263e-61	-8.615850e-47
Msa1236360	Msa1299400	-0.819121	1.371289e-52	-8.615850e-47
Msa1236360	Msa1308870	0.825334	4.960350e-54	-8.615850e-47
Msa1236360	Msa1316420	0.823733	1.181285e-53	-8.615850e-47
Msa1236360	Msa1325410	-0.822367	2.460619e-53	-8.615850e-47
Msa1236360	Msa1331000	0.801340	9.429006e-49	-8.615850e-47
Msa1236360	Msa1331050	-0.805969	1.032776e-49	-8.615850e-47
Msa1236360	Msa1342380	0.804907	1.724030e-49	-8.615850e-47
Msa0367870	Msa1236360	0.826722	2.321059e-54	-8.615850e-47
Msa0403640	Msa1236360	0.800049	1.728947e-48	-8.615850e-47
Msa0460590	Msa1236360	0.804917	1.715773e-49	-8.615850e-47
Msa0999680	Msa1236360	0.835194	1.934381e-56	-8.615850e-47
Msa0287740	Msa1236360	0.810861	9.340029e-51	-8.615850e-47
Msa0363790	Msa1236360	0.836941	6.963852e-57	-8.615850e-47
Msa1147740	Msa1236360	0.801993	6.926286e-49	-8.615850e-47
Msa1211230	Msa1236360	-0.812905	3.350389e-51	-8.615850e-47
Msa0486690	Msa1236360	0.819496	1.126587e-52	-8.615850e-47
Msa0548120	Msa1236360	0.804137	2.495792e-49	-8.615850e-47
Msa0858390	Msa1236360	0.800285	1.548288e-48	-8.615850e-47
Msa0899970	Msa1236360	0.822511	2.277624e-53	-8.615850e-47
Msa0965080	Msa1236360	0.842423	2.609338e-58	-8.615850e-47
Msa0965290	Msa1236360	0.840794	7.015309e-58	-8.615850e-47
Msa0967870	Msa1236360	0.809587	1.757911e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1236360	MtrunA17_Chr8g0346501	100.000	106	0	0	1	106	1	106	3.26e-77	222
Msa1236360	MtrunA17_Chr3g0127211	67.619	105	34	0	1	105	1	105	1.43e-50	155
Msa1236360	MtrunA17_Chr1g0188931	63.725	102	37	0	1	102	1	102	1.13e-47	147
Msa1236360	MtrunA17_Chr5g0436971	50.505	99	49	0	8	106	12	110	1.38e-33	113
Msa1236360	MtrunA17_Chr3g0113291	52.128	94	45	0	8	101	35	128	1.86e-33	113
Msa1236360	MtrunA17_Chr4g0046851	50.000	100	49	1	3	102	8	106	8.28e-31	105
Msa1236360	MtrunA17_Chr7g0242231	48.454	97	50	0	10	106	14	110	4.40e-30	103
Msa1236360	MtrunA17_Chr2g0316921	48.421	95	49	0	8	102	12	106	7.52e-29	100
Msa1236360	MtrunA17_Chr8g0360291	44.211	95	53	0	8	102	12	106	1.91e-26	95.1
Msa1236360	MtrunA17_Chr1g0188951	64.516	62	22	0	44	105	1	62	2.60e-22	82.4
Msa1236360	MtrunA17_Chr2g0316891	50.794	63	31	0	40	102	42	104	1.40e-18	74.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1236360	AT4G27745.2	66.667	102	34	0	1	102	1	102	1.63e-47	147
Msa1236360	AT4G27745.3	66.667	102	34	0	1	102	1	102	1.63e-47	147
Msa1236360	AT4G27745.1	66.667	102	34	0	1	102	1	102	1.63e-47	147
Msa1236360	AT4G27745.4	66.667	102	34	0	1	102	1	102	1.63e-47	147
Msa1236360	AT3G11230.1	50.505	99	49	0	8	106	12	110	1.23e-34	115
Msa1236360	AT3G11230.2	50.505	99	49	0	8	106	45	143	4.47e-34	115
Msa1236360	AT3G11230.4	52.174	92	44	0	8	99	12	103	1.76e-33	112
Msa1236360	AT4G27740.1	51.064	94	46	0	9	102	9	102	2.71e-33	112
Msa1236360	AT3G55890.4	52.475	101	47	1	1	101	6	105	3.21e-33	112
Msa1236360	AT3G55890.1	52.475	101	47	1	1	101	6	105	3.21e-33	112
Msa1236360	AT3G11230.3	52.747	91	43	0	8	98	45	135	6.02e-33	112
Msa1236360	AT5G53940.1	50.526	95	47	0	8	102	12	106	2.25e-32	110
Msa1236360	AT2G40110.1	49.495	99	50	0	8	106	12	110	3.91e-32	109
Msa1236360	AT3G55890.3	52.577	97	45	1	1	97	6	101	4.46e-32	108
Msa1236360	AT3G55890.2	52.577	97	45	1	1	97	6	101	4.46e-32	108
Msa1236360	AT3G08990.2	50.000	102	50	1	1	102	6	106	9.04e-32	108
Msa1236360	AT3G08990.1	50.000	102	50	1	1	102	6	106	9.04e-32	108
Msa1236360	AT3G08990.3	50.000	102	50	1	1	102	6	106	9.04e-32	108
Msa1236360	AT2G40110.2	50.000	92	46	0	8	99	12	103	8.94e-31	105
Msa1236360	AT2G40110.4	45.205	73	40	0	8	80	12	84	1.29e-21	82.0
Msa1236360	AT2G40110.3	45.205	73	40	0	8	80	12	84	1.29e-21	82.0

Find 41 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGTTTCCTCATAAGCTTTA+AGG	0.102549	8_2:+15278280	None:intergenic
TTGTAAATGTTGCTTGATTA+GGG	0.247484	8_2:-15278113	Msa1236360:three_prime_UTR
TGAGAAGTTTAAGATTGTTT+TGG	0.258269	8_2:-15278229	Msa1236360:CDS
AAAGTTCACCTGAAAATCTT+TGG	0.258418	8_2:+15279181	None:intergenic
ATAAGCTGTCTATCTTCTTT+AGG	0.260634	8_2:+15278369	None:intergenic
TCATGTCAGAGTTGGATAAT+TGG	0.261612	8_2:+15279450	None:intergenic
TTTGTAAATGTTGCTTGATT+AGG	0.280982	8_2:-15278114	Msa1236360:three_prime_UTR
TTTAACATTAATTGTTCATT+GGG	0.318516	8_2:-15278181	Msa1236360:three_prime_UTR
ATCATTAATGGCCGATTTGA+TGG	0.329137	8_2:-15279267	Msa1236360:exon
ACCATTCAACAATTAACATT+AGG	0.330744	8_2:+15278017	None:intergenic
CCATTCAACAATTAACATTA+GGG	0.341166	8_2:+15278018	None:intergenic
AAAGAAGATTCCAAAAGAAT+TGG	0.353208	8_2:+15279554	None:intergenic
CATGGAAAGCAACAAGGTTC+TGG	0.376925	8_2:+15279216	None:intergenic
GGAGCTAAGGTGGCAGTGTT+AGG	0.379271	8_2:-15279297	Msa1236360:five_prime_UTR
TGAGGAAACACAGAAGTATA+AGG	0.390368	8_2:-15278268	Msa1236360:CDS
CCCTAATGTTAATTGTTGAA+TGG	0.397010	8_2:-15278018	Msa1236360:three_prime_UTR
GAAATACCTTAAAGCTTATG+AGG	0.433394	8_2:-15278286	Msa1236360:CDS
CAACTCTGACATGAAAAGAT+TGG	0.449933	8_2:-15279441	Msa1236360:five_prime_UTR
CAACAAACCTTCTCTGTACA+AGG	0.478519	8_2:+15277931	None:intergenic
GCAAAGAATATGAATGAGAA+GGG	0.486362	8_2:+15279522	None:intergenic
GGCCGATTTGATGGGATCAC+GGG	0.488237	8_2:-15279258	Msa1236360:CDS
TCAGAGTTGGATAATTGGTT+TGG	0.496251	8_2:+15279455	None:intergenic
TGCCCGTGATCCCATCAAAT+CGG	0.500160	8_2:+15279256	None:intergenic
TTATTAGCCTTGTACAGAGA+AGG	0.526516	8_2:-15277938	Msa1236360:three_prime_UTR
TCATTAATGGCCGATTTGAT+GGG	0.534012	8_2:-15279266	Msa1236360:exon
TGGCCGATTTGATGGGATCA+CGG	0.537450	8_2:-15279259	Msa1236360:CDS
TATGTTTAACAGGCAAGCAA+TGG	0.540111	8_2:-15278435	Msa1236360:intron
CTTTGGAAACAATATCATCA+TGG	0.545782	8_2:+15279198	None:intergenic
AGCAAAGAATATGAATGAGA+AGG	0.555819	8_2:+15279521	None:intergenic
TCTGATTGTGGAGAAGAACT+TGG	0.556503	8_2:-15278312	Msa1236360:CDS
ATTGTGGAGAAGAACTTGGT+TGG	0.558548	8_2:-15278308	Msa1236360:CDS
GAAGATAGACAGCTTATGAC+TGG	0.563935	8_2:-15278363	Msa1236360:CDS
TTATGTATGAGTAGGAGCTA+AGG	0.582837	8_2:-15279310	Msa1236360:intron
TTATACTAATGTTGTACATG+TGG	0.589916	8_2:+15278153	None:intergenic
TGTAAATGTTGCTTGATTAG+GGG	0.592001	8_2:-15278112	Msa1236360:three_prime_UTR
GAAACACAGAAGTATAAGGA+AGG	0.612715	8_2:-15278264	Msa1236360:CDS
TGTATGAGTAGGAGCTAAGG+TGG	0.621962	8_2:-15279307	Msa1236360:intron
TATCATCATGGAAAGCAACA+AGG	0.636612	8_2:+15279210	None:intergenic
TTAACATTAATTGTTCATTG+GGG	0.648404	8_2:-15278180	Msa1236360:three_prime_UTR
GTACAATATGAACAAGAACA+AGG	0.661988	8_2:+15277994	None:intergenic
CATTCAACAATTAACATTAG+GGG	0.686208	8_2:+15278019	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAAATTTGTTTAAATCAAA+TGG	-	chr8_2:15278468-15278487	Msa1236360:intron	10.0%
!!!	AATTTACAATATGATTTTTT+TGG	+	chr8_2:15278876-15278895	None:intergenic	10.0%
!!!	TTAATTTGTTGAATATTAAT+TGG	-	chr8_2:15278973-15278992	Msa1236360:intron	10.0%
!!!	AAAAATTTGTTTAAATCAAA+TGG	-	chr8_2:15278468-15278487	Msa1236360:intron	10.0%
!!!	AATTTACAATATGATTTTTT+TGG	+	chr8_2:15278876-15278895	None:intergenic	10.0%
!!!	TTAATTTGTTGAATATTAAT+TGG	-	chr8_2:15278973-15278992	Msa1236360:intron	10.0%
!!	TATATTATTTATGTATGAGT+AGG	-	chr8_2:15278176-15278195	Msa1236360:three_prime_UTR	15.0%
!!	TTAGGAAAAAAAATCATTAA+TGG	-	chr8_2:15278215-15278234	Msa1236360:exon	15.0%
!!!	AATTTTTTCTCTAAATCTAA+CGG	+	chr8_2:15278455-15278474	None:intergenic	15.0%
!!!	TTTTAACATTAATTGTTCAT+TGG	-	chr8_2:15279312-15279331	Msa1236360:intron	15.0%
!!	TTTAACATTAATTGTTCATT+GGG	-	chr8_2:15279313-15279332	Msa1236360:intron	15.0%
!!!	TTTTATTCACTCAAAAAAAT+TGG	-	chr8_2:15279416-15279435	Msa1236360:intron	15.0%
!!	TATATTATTTATGTATGAGT+AGG	-	chr8_2:15278176-15278195	Msa1236360:three_prime_UTR	15.0%
!!	TTAGGAAAAAAAATCATTAA+TGG	-	chr8_2:15278215-15278234	Msa1236360:exon	15.0%
!!!	AATTTTTTCTCTAAATCTAA+CGG	+	chr8_2:15278455-15278474	None:intergenic	15.0%
!!!	TTTTAACATTAATTGTTCAT+TGG	-	chr8_2:15279312-15279331	Msa1236360:intron	15.0%
!!	TTTAACATTAATTGTTCATT+GGG	-	chr8_2:15279313-15279332	Msa1236360:intron	15.0%
!!!	TTTTATTCACTCAAAAAAAT+TGG	-	chr8_2:15279416-15279435	Msa1236360:intron	15.0%
!!	AAGAATTGGACAAAAAATAT+TGG	+	chr8_2:15277929-15277948	None:intergenic	20.0%
!!!	TATTTTTTGTCCAATTCTTT+TGG	-	chr8_2:15277930-15277949	Msa1236360:three_prime_UTR	20.0%
!!	AGTATATAAAAGAAAAAGCT+TGG	+	chr8_2:15278119-15278138	None:intergenic	20.0%
!!!	ACTTTTCTTTCTTTCAATTT+TGG	-	chr8_2:15278331-15278350	Msa1236360:CDS	20.0%
!!	TTAACATTAATTGTTCATTG+GGG	-	chr8_2:15279314-15279333	Msa1236360:intron	20.0%
!!	AAGAATTGGACAAAAAATAT+TGG	+	chr8_2:15277929-15277948	None:intergenic	20.0%
!!!	TATTTTTTGTCCAATTCTTT+TGG	-	chr8_2:15277930-15277949	Msa1236360:three_prime_UTR	20.0%
!!	AGTATATAAAAGAAAAAGCT+TGG	+	chr8_2:15278119-15278138	None:intergenic	20.0%
!!!	ACTTTTCTTTCTTTCAATTT+TGG	-	chr8_2:15278331-15278350	Msa1236360:CDS	20.0%
!!	TTAACATTAATTGTTCATTG+GGG	-	chr8_2:15279314-15279333	Msa1236360:intron	20.0%
!	AAAGAAGATTCCAAAAGAAT+TGG	+	chr8_2:15277943-15277962	None:intergenic	25.0%
!!	TATTGTTTCCAAAGATTTTC+AGG	-	chr8_2:15278305-15278324	Msa1236360:CDS	25.0%
!!	TATTAGATGATCCATTTTCA+TGG	+	chr8_2:15278404-15278423	None:intergenic	25.0%
!	AGAGAAGTGAAATTAATTTC+AGG	+	chr8_2:15278686-15278705	None:intergenic	25.0%
!	ATTAATTTCACTTCTCTCAA+TGG	-	chr8_2:15278689-15278708	Msa1236360:intron	25.0%
!	TTCATTTCTTCTGATCAATT+CGG	+	chr8_2:15278767-15278786	None:intergenic	25.0%
!	AGAAGAAATGAAACAATCAT+TGG	-	chr8_2:15278775-15278794	Msa1236360:intron	25.0%
!	TTGAATATTAATTGGACAAG+AGG	-	chr8_2:15278981-15279000	Msa1236360:intron	25.0%
!	TGAATATTAATTGGACAAGA+GGG	-	chr8_2:15278982-15279001	Msa1236360:intron	25.0%
!!	TAACCTCAAAAGATTTTCAA+AGG	-	chr8_2:15279035-15279054	Msa1236360:intron	25.0%
!!!	TAACCTTTGAAAATCTTTTG+AGG	+	chr8_2:15279041-15279060	None:intergenic	25.0%
!	TTTCAAAGGTTATGTTTAAC+AGG	-	chr8_2:15279049-15279068	Msa1236360:intron	25.0%
!!!	TGAGAAGTTTAAGATTGTTT+TGG	-	chr8_2:15279265-15279284	Msa1236360:exon	25.0%
!!!	GAGAAGTTTAAGATTGTTTT+GGG	-	chr8_2:15279266-15279285	Msa1236360:exon	25.0%
!!!	TTTAAGATTGTTTTGGGAAA+TGG	-	chr8_2:15279272-15279291	Msa1236360:exon	25.0%
!!!	TTAAGATTGTTTTGGGAAAT+GGG	-	chr8_2:15279273-15279292	Msa1236360:exon	25.0%
!	TTATACTAATGTTGTACATG+TGG	+	chr8_2:15279344-15279363	None:intergenic	25.0%
!!	TTTGTAAATGTTGCTTGATT+AGG	-	chr8_2:15279380-15279399	Msa1236360:intron	25.0%
!!	TTGTAAATGTTGCTTGATTA+GGG	-	chr8_2:15279381-15279400	Msa1236360:intron	25.0%
!	CATTCAACAATTAACATTAG+GGG	+	chr8_2:15279478-15279497	None:intergenic	25.0%
!	CCATTCAACAATTAACATTA+GGG	+	chr8_2:15279479-15279498	None:intergenic	25.0%
!	ACCATTCAACAATTAACATT+AGG	+	chr8_2:15279480-15279499	None:intergenic	25.0%
!	AAAGAAGATTCCAAAAGAAT+TGG	+	chr8_2:15277943-15277962	None:intergenic	25.0%
!!	TATTGTTTCCAAAGATTTTC+AGG	-	chr8_2:15278305-15278324	Msa1236360:CDS	25.0%
!!	TATTAGATGATCCATTTTCA+TGG	+	chr8_2:15278404-15278423	None:intergenic	25.0%
!	AGAGAAGTGAAATTAATTTC+AGG	+	chr8_2:15278686-15278705	None:intergenic	25.0%
!	ATTAATTTCACTTCTCTCAA+TGG	-	chr8_2:15278689-15278708	Msa1236360:intron	25.0%
!	TTCATTTCTTCTGATCAATT+CGG	+	chr8_2:15278767-15278786	None:intergenic	25.0%
!	AGAAGAAATGAAACAATCAT+TGG	-	chr8_2:15278775-15278794	Msa1236360:intron	25.0%
!	TTGAATATTAATTGGACAAG+AGG	-	chr8_2:15278981-15279000	Msa1236360:intron	25.0%
!	TGAATATTAATTGGACAAGA+GGG	-	chr8_2:15278982-15279001	Msa1236360:intron	25.0%
!!	TAACCTCAAAAGATTTTCAA+AGG	-	chr8_2:15279035-15279054	Msa1236360:intron	25.0%
!!!	TAACCTTTGAAAATCTTTTG+AGG	+	chr8_2:15279041-15279060	None:intergenic	25.0%
!	TTTCAAAGGTTATGTTTAAC+AGG	-	chr8_2:15279049-15279068	Msa1236360:intron	25.0%
!!!	TGAGAAGTTTAAGATTGTTT+TGG	-	chr8_2:15279265-15279284	Msa1236360:exon	25.0%
!!!	GAGAAGTTTAAGATTGTTTT+GGG	-	chr8_2:15279266-15279285	Msa1236360:exon	25.0%
!!!	TTTAAGATTGTTTTGGGAAA+TGG	-	chr8_2:15279272-15279291	Msa1236360:exon	25.0%
!!!	TTAAGATTGTTTTGGGAAAT+GGG	-	chr8_2:15279273-15279292	Msa1236360:exon	25.0%
!	TTATACTAATGTTGTACATG+TGG	+	chr8_2:15279344-15279363	None:intergenic	25.0%
!!	TTTGTAAATGTTGCTTGATT+AGG	-	chr8_2:15279380-15279399	Msa1236360:intron	25.0%
!!	TTGTAAATGTTGCTTGATTA+GGG	-	chr8_2:15279381-15279400	Msa1236360:intron	25.0%
!	CATTCAACAATTAACATTAG+GGG	+	chr8_2:15279478-15279497	None:intergenic	25.0%
!	CCATTCAACAATTAACATTA+GGG	+	chr8_2:15279479-15279498	None:intergenic	25.0%
!	ACCATTCAACAATTAACATT+AGG	+	chr8_2:15279480-15279499	None:intergenic	25.0%
	GCAAAGAATATGAATGAGAA+GGG	+	chr8_2:15277975-15277994	None:intergenic	30.0%
	AGCAAAGAATATGAATGAGA+AGG	+	chr8_2:15277976-15277995	None:intergenic	30.0%
!	CTTTGGAAACAATATCATCA+TGG	+	chr8_2:15278299-15278318	None:intergenic	30.0%
	AAAGTTCACCTGAAAATCTT+TGG	+	chr8_2:15278316-15278335	None:intergenic	30.0%
	AAACGATTCATCCATGAAAA+TGG	-	chr8_2:15278390-15278409	Msa1236360:CDS	30.0%
	AAATGGATCATCTAATACTG+TGG	-	chr8_2:15278407-15278426	Msa1236360:CDS	30.0%
	GCTTTCATCGTGATTATATT+GGG	+	chr8_2:15278652-15278671	None:intergenic	30.0%
	AGCTTTCATCGTGATTATAT+TGG	+	chr8_2:15278653-15278672	None:intergenic	30.0%
	TAAAGGAAATGGACAAAATC+CGG	+	chr8_2:15278830-15278849	None:intergenic	30.0%
!!!	TTGGTTTTAATTTAACCCTC+TGG	+	chr8_2:15278857-15278876	None:intergenic	30.0%
!	TCATGCCATGAACATATTTT+TGG	-	chr8_2:15279100-15279119	Msa1236360:intron	30.0%
!	CATGCCATGAACATATTTTT+GGG	-	chr8_2:15279101-15279120	Msa1236360:intron	30.0%
	ATAAGCTGTCTATCTTCTTT+AGG	+	chr8_2:15279128-15279147	None:intergenic	30.0%
!!!	GATGTTTTTTGTTCTGATTG+TGG	-	chr8_2:15279170-15279189	Msa1236360:intron	30.0%
	GAAATACCTTAAAGCTTATG+AGG	-	chr8_2:15279208-15279227	Msa1236360:CDS	30.0%
!	TGTAAATGTTGCTTGATTAG+GGG	-	chr8_2:15279382-15279401	Msa1236360:intron	30.0%
	CCCTAATGTTAATTGTTGAA+TGG	-	chr8_2:15279476-15279495	Msa1236360:five_prime_UTR	30.0%
	GTACAATATGAACAAGAACA+AGG	+	chr8_2:15279503-15279522	None:intergenic	30.0%
	GCAAAGAATATGAATGAGAA+GGG	+	chr8_2:15277975-15277994	None:intergenic	30.0%
	AGCAAAGAATATGAATGAGA+AGG	+	chr8_2:15277976-15277995	None:intergenic	30.0%
!	CTTTGGAAACAATATCATCA+TGG	+	chr8_2:15278299-15278318	None:intergenic	30.0%
	AAAGTTCACCTGAAAATCTT+TGG	+	chr8_2:15278316-15278335	None:intergenic	30.0%
	AAACGATTCATCCATGAAAA+TGG	-	chr8_2:15278390-15278409	Msa1236360:CDS	30.0%
	AAATGGATCATCTAATACTG+TGG	-	chr8_2:15278407-15278426	Msa1236360:CDS	30.0%
	GCTTTCATCGTGATTATATT+GGG	+	chr8_2:15278652-15278671	None:intergenic	30.0%
	AGCTTTCATCGTGATTATAT+TGG	+	chr8_2:15278653-15278672	None:intergenic	30.0%
	TAAAGGAAATGGACAAAATC+CGG	+	chr8_2:15278830-15278849	None:intergenic	30.0%
!!!	TTGGTTTTAATTTAACCCTC+TGG	+	chr8_2:15278857-15278876	None:intergenic	30.0%
!	TCATGCCATGAACATATTTT+TGG	-	chr8_2:15279100-15279119	Msa1236360:intron	30.0%
!	CATGCCATGAACATATTTTT+GGG	-	chr8_2:15279101-15279120	Msa1236360:intron	30.0%
	ATAAGCTGTCTATCTTCTTT+AGG	+	chr8_2:15279128-15279147	None:intergenic	30.0%
!!!	GATGTTTTTTGTTCTGATTG+TGG	-	chr8_2:15279170-15279189	Msa1236360:intron	30.0%
	GAAATACCTTAAAGCTTATG+AGG	-	chr8_2:15279208-15279227	Msa1236360:CDS	30.0%
!	TGTAAATGTTGCTTGATTAG+GGG	-	chr8_2:15279382-15279401	Msa1236360:intron	30.0%
	CCCTAATGTTAATTGTTGAA+TGG	-	chr8_2:15279476-15279495	Msa1236360:five_prime_UTR	30.0%
	GTACAATATGAACAAGAACA+AGG	+	chr8_2:15279503-15279522	None:intergenic	30.0%
	TCAGAGTTGGATAATTGGTT+TGG	+	chr8_2:15278042-15278061	None:intergenic	35.0%
	TCATGTCAGAGTTGGATAAT+TGG	+	chr8_2:15278047-15278066	None:intergenic	35.0%
!	CAATCTTTTCATGTCAGAGT+TGG	+	chr8_2:15278055-15278074	None:intergenic	35.0%
	CAACTCTGACATGAAAAGAT+TGG	-	chr8_2:15278053-15278072	Msa1236360:three_prime_UTR	35.0%
	TTATGTATGAGTAGGAGCTA+AGG	-	chr8_2:15278184-15278203	Msa1236360:three_prime_UTR	35.0%
!!	ATCATTAATGGCCGATTTGA+TGG	-	chr8_2:15278227-15278246	Msa1236360:CDS	35.0%
!!	TCATTAATGGCCGATTTGAT+GGG	-	chr8_2:15278228-15278247	Msa1236360:CDS	35.0%
	TATCATCATGGAAAGCAACA+AGG	+	chr8_2:15278287-15278306	None:intergenic	35.0%
!	AAAATGTCTTCTAGTGGGTA+TGG	+	chr8_2:15278545-15278564	None:intergenic	35.0%
!!!	ACCCACTAGAAGACATTTTT+TGG	-	chr8_2:15278546-15278565	Msa1236360:intron	35.0%
	CCCAAAAAATGTCTTCTAGT+GGG	+	chr8_2:15278550-15278569	None:intergenic	35.0%
!!!	CCCACTAGAAGACATTTTTT+GGG	-	chr8_2:15278547-15278566	Msa1236360:intron	35.0%
	ACCCAAAAAATGTCTTCTAG+TGG	+	chr8_2:15278551-15278570	None:intergenic	35.0%
	TGCTTAACGAGCTTCAATTA+AGG	-	chr8_2:15278741-15278760	Msa1236360:intron	35.0%
!	GGATTTTGTCCATTTCCTTT+AGG	-	chr8_2:15278829-15278848	Msa1236360:intron	35.0%
	ATATTAATTGGACAAGAGGG+TGG	-	chr8_2:15278985-15279004	Msa1236360:intron	35.0%
	TATGTTTAACAGGCAAGCAA+TGG	-	chr8_2:15279059-15279078	Msa1236360:intron	35.0%
	AGGTCCCAAAAATATGTTCA+TGG	+	chr8_2:15279108-15279127	None:intergenic	35.0%
	GTGTTTCCTCATAAGCTTTA+AGG	+	chr8_2:15279217-15279236	None:intergenic	35.0%
!	TGAGGAAACACAGAAGTATA+AGG	-	chr8_2:15279226-15279245	Msa1236360:CDS	35.0%
!	GAAACACAGAAGTATAAGGA+AGG	-	chr8_2:15279230-15279249	Msa1236360:CDS	35.0%
!!	TTATTAGCCTTGTACAGAGA+AGG	-	chr8_2:15279556-15279575	Msa1236360:five_prime_UTR	35.0%
	TCAGAGTTGGATAATTGGTT+TGG	+	chr8_2:15278042-15278061	None:intergenic	35.0%
	TCATGTCAGAGTTGGATAAT+TGG	+	chr8_2:15278047-15278066	None:intergenic	35.0%
!	CAATCTTTTCATGTCAGAGT+TGG	+	chr8_2:15278055-15278074	None:intergenic	35.0%
	CAACTCTGACATGAAAAGAT+TGG	-	chr8_2:15278053-15278072	Msa1236360:three_prime_UTR	35.0%
	TTATGTATGAGTAGGAGCTA+AGG	-	chr8_2:15278184-15278203	Msa1236360:three_prime_UTR	35.0%
!!	ATCATTAATGGCCGATTTGA+TGG	-	chr8_2:15278227-15278246	Msa1236360:CDS	35.0%
!!	TCATTAATGGCCGATTTGAT+GGG	-	chr8_2:15278228-15278247	Msa1236360:CDS	35.0%
	TATCATCATGGAAAGCAACA+AGG	+	chr8_2:15278287-15278306	None:intergenic	35.0%
!	AAAATGTCTTCTAGTGGGTA+TGG	+	chr8_2:15278545-15278564	None:intergenic	35.0%
!!!	ACCCACTAGAAGACATTTTT+TGG	-	chr8_2:15278546-15278565	Msa1236360:intron	35.0%
	CCCAAAAAATGTCTTCTAGT+GGG	+	chr8_2:15278550-15278569	None:intergenic	35.0%
!!!	CCCACTAGAAGACATTTTTT+GGG	-	chr8_2:15278547-15278566	Msa1236360:intron	35.0%
	ACCCAAAAAATGTCTTCTAG+TGG	+	chr8_2:15278551-15278570	None:intergenic	35.0%
	TGCTTAACGAGCTTCAATTA+AGG	-	chr8_2:15278741-15278760	Msa1236360:intron	35.0%
!	GGATTTTGTCCATTTCCTTT+AGG	-	chr8_2:15278829-15278848	Msa1236360:intron	35.0%
	ATATTAATTGGACAAGAGGG+TGG	-	chr8_2:15278985-15279004	Msa1236360:intron	35.0%
	TATGTTTAACAGGCAAGCAA+TGG	-	chr8_2:15279059-15279078	Msa1236360:intron	35.0%
	AGGTCCCAAAAATATGTTCA+TGG	+	chr8_2:15279108-15279127	None:intergenic	35.0%
	GTGTTTCCTCATAAGCTTTA+AGG	+	chr8_2:15279217-15279236	None:intergenic	35.0%
!	TGAGGAAACACAGAAGTATA+AGG	-	chr8_2:15279226-15279245	Msa1236360:CDS	35.0%
!	GAAACACAGAAGTATAAGGA+AGG	-	chr8_2:15279230-15279249	Msa1236360:CDS	35.0%
!!	TTATTAGCCTTGTACAGAGA+AGG	-	chr8_2:15279556-15279575	Msa1236360:five_prime_UTR	35.0%
	AATCAAATGGTTCCTACCAC+AGG	-	chr8_2:15278481-15278500	Msa1236360:intron	40.0%
	ATCAAATGGTTCCTACCACA+GGG	-	chr8_2:15278482-15278501	Msa1236360:intron	40.0%
	TAAGTAGAAGAACATCCCTG+TGG	+	chr8_2:15278500-15278519	None:intergenic	40.0%
	CAGGGATGTTCTTCTACTTA+TGG	-	chr8_2:15278500-15278519	Msa1236360:intron	40.0%
	TGGGTATGGATGAACGAAAT+TGG	+	chr8_2:15278531-15278550	None:intergenic	40.0%
	CTTACTTTGTGACTGAACTC+CGG	-	chr8_2:15278808-15278827	Msa1236360:intron	40.0%
!	CATTTCCTTTAGGAACCAGA+GGG	-	chr8_2:15278839-15278858	Msa1236360:intron	40.0%
!	TTTAACCCTCTGGTTCCTAA+AGG	+	chr8_2:15278847-15278866	None:intergenic	40.0%
	GAAGATAGACAGCTTATGAC+TGG	-	chr8_2:15279131-15279150	Msa1236360:intron	40.0%
	TCTGATTGTGGAGAAGAACT+TGG	-	chr8_2:15279182-15279201	Msa1236360:intron	40.0%
	ATTGTGGAGAAGAACTTGGT+TGG	-	chr8_2:15279186-15279205	Msa1236360:intron	40.0%
	CAACAAACCTTCTCTGTACA+AGG	+	chr8_2:15279566-15279585	None:intergenic	40.0%
	AATCAAATGGTTCCTACCAC+AGG	-	chr8_2:15278481-15278500	Msa1236360:intron	40.0%
	ATCAAATGGTTCCTACCACA+GGG	-	chr8_2:15278482-15278501	Msa1236360:intron	40.0%
	TAAGTAGAAGAACATCCCTG+TGG	+	chr8_2:15278500-15278519	None:intergenic	40.0%
	CAGGGATGTTCTTCTACTTA+TGG	-	chr8_2:15278500-15278519	Msa1236360:intron	40.0%
	TGGGTATGGATGAACGAAAT+TGG	+	chr8_2:15278531-15278550	None:intergenic	40.0%
	CTTACTTTGTGACTGAACTC+CGG	-	chr8_2:15278808-15278827	Msa1236360:intron	40.0%
!	CATTTCCTTTAGGAACCAGA+GGG	-	chr8_2:15278839-15278858	Msa1236360:intron	40.0%
!	TTTAACCCTCTGGTTCCTAA+AGG	+	chr8_2:15278847-15278866	None:intergenic	40.0%
	GAAGATAGACAGCTTATGAC+TGG	-	chr8_2:15279131-15279150	Msa1236360:intron	40.0%
	TCTGATTGTGGAGAAGAACT+TGG	-	chr8_2:15279182-15279201	Msa1236360:intron	40.0%
	ATTGTGGAGAAGAACTTGGT+TGG	-	chr8_2:15279186-15279205	Msa1236360:intron	40.0%
	CAACAAACCTTCTCTGTACA+AGG	+	chr8_2:15279566-15279585	None:intergenic	40.0%
	TGTATGAGTAGGAGCTAAGG+TGG	-	chr8_2:15278187-15278206	Msa1236360:three_prime_UTR	45.0%
	CATGGAAAGCAACAAGGTTC+TGG	+	chr8_2:15278281-15278300	None:intergenic	45.0%
!	ACTGTGGAATAGTGTTGCTG+CGG	-	chr8_2:15278423-15278442	Msa1236360:CDS	45.0%
	TAGAAGAACATCCCTGTGGT+AGG	+	chr8_2:15278496-15278515	None:intergenic	45.0%
!	CCTCTGGTTCCTAAAGGAAA+TGG	+	chr8_2:15278841-15278860	None:intergenic	45.0%
!	CCATTTCCTTTAGGAACCAG+AGG	-	chr8_2:15278838-15278857	Msa1236360:intron	45.0%
	TAATTGGACAAGAGGGTGGT+TGG	-	chr8_2:15278989-15279008	Msa1236360:intron	45.0%
	TGTATGAGTAGGAGCTAAGG+TGG	-	chr8_2:15278187-15278206	Msa1236360:three_prime_UTR	45.0%
	CATGGAAAGCAACAAGGTTC+TGG	+	chr8_2:15278281-15278300	None:intergenic	45.0%
!	ACTGTGGAATAGTGTTGCTG+CGG	-	chr8_2:15278423-15278442	Msa1236360:CDS	45.0%
	TAGAAGAACATCCCTGTGGT+AGG	+	chr8_2:15278496-15278515	None:intergenic	45.0%
!	CCTCTGGTTCCTAAAGGAAA+TGG	+	chr8_2:15278841-15278860	None:intergenic	45.0%
!	CCATTTCCTTTAGGAACCAG+AGG	-	chr8_2:15278838-15278857	Msa1236360:intron	45.0%
	TAATTGGACAAGAGGGTGGT+TGG	-	chr8_2:15278989-15279008	Msa1236360:intron	45.0%
!!	TGGCCGATTTGATGGGATCA+CGG	-	chr8_2:15278235-15278254	Msa1236360:CDS	50.0%
	TGCCCGTGATCCCATCAAAT+CGG	+	chr8_2:15278241-15278260	None:intergenic	50.0%
!!	TGGCCGATTTGATGGGATCA+CGG	-	chr8_2:15278235-15278254	Msa1236360:CDS	50.0%
	TGCCCGTGATCCCATCAAAT+CGG	+	chr8_2:15278241-15278260	None:intergenic	50.0%
	GGAGCTAAGGTGGCAGTGTT+AGG	-	chr8_2:15278197-15278216	Msa1236360:three_prime_UTR	55.0%
!!	GGCCGATTTGATGGGATCAC+GGG	-	chr8_2:15278236-15278255	Msa1236360:CDS	55.0%
	GGAGCTAAGGTGGCAGTGTT+AGG	-	chr8_2:15278197-15278216	Msa1236360:three_prime_UTR	55.0%
!!	GGCCGATTTGATGGGATCAC+GGG	-	chr8_2:15278236-15278255	Msa1236360:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	15277924	15279592	15277924	ID=Msa1236360;Name=Msa1236360
chr8_2	mRNA	15277924	15279592	15277924	ID=Msa1236360-mRNA-1;Parent=Msa1236360;Name=Msa1236360-mRNA-1;_AED=0.24;_eAED=0.24;_QI=212\|1\|1\|1\|0.5\|0.33\|3\|294\|106
chr8_2	exon	15277924	15278445	15277924	ID=Msa1236360-mRNA-1:exon:5829;Parent=Msa1236360-mRNA-1
chr8_2	exon	15279190	15279318	15279190	ID=Msa1236360-mRNA-1:exon:5828;Parent=Msa1236360-mRNA-1
chr8_2	exon	15279417	15279592	15279417	ID=Msa1236360-mRNA-1:exon:5827;Parent=Msa1236360-mRNA-1
chr8_2	five_prime_UTR	15279417	15279592	15279417	ID=Msa1236360-mRNA-1:five_prime_utr;Parent=Msa1236360-mRNA-1
chr8_2	five_prime_UTR	15279283	15279318	15279283	ID=Msa1236360-mRNA-1:five_prime_utr;Parent=Msa1236360-mRNA-1
chr8_2	CDS	15279190	15279282	15279190	ID=Msa1236360-mRNA-1:cds;Parent=Msa1236360-mRNA-1
chr8_2	CDS	15278218	15278445	15278218	ID=Msa1236360-mRNA-1:cds;Parent=Msa1236360-mRNA-1
chr8_2	three_prime_UTR	15277924	15278217	15277924	ID=Msa1236360-mRNA-1:three_prime_utr;Parent=Msa1236360-mRNA-1

Gene Sequence	>Msa1236360 ATGGCCGATTTGATGGGATCACGGGCATACTGCTGCTTCAAGTGCCAGAACCTTGTTGCTTTCCATGATGATATTGTTTCCAAAGATTTTCAGGCAAGCAATGGAAGAGCTTTTCTGTTTTCTCATGCCATGAACATATTTTTGGGACCTAAAGAAGATAGACAGCTTATGACTGGTCTTCACACAGTTGCTGATGTTTTTTGTTCTGATTGTGGAGAAGAACTTGGTTGGAAATACCTTAAAGCTTATGAGGAAACACAGAAGTATAAGGAAGGAAAATGTGTGCTTGAGAAGTTTAAGATTGTTTTGGGAAATGGGTAG
Protein sequence	>Msa1236360 MADLMGSRAYCCFKCQNLVAFHDDIVSKDFQASNGRAFLFSHAMNIFLGPKEDRQLMTGLHTVADVFCSDCGEELGWKYLKAYEETQKYKEGKCVLEKFKIVLGNG*