Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1321080 | MCI04099.1 | 63.235 | 136 | 39 | 2 | 18 | 142 | 1 | 136 | 2.77e-44 | 153 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1321080 | A0A392NY20 | 63.235 | 136 | 39 | 2 | 18 | 142 | 1 | 136 | 1.32e-44 | 153 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0311750 | Msa1321080 | 0.887882 | 9.654603e-73 | -8.615850e-47 |
| Msa0877250 | Msa1321080 | 0.832698 | 8.150806e-56 | -8.615850e-47 |
| Msa0974440 | Msa1321080 | 0.833378 | 5.520025e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1321080 | MtrunA17_Chr4g0059881 | 66.667 | 138 | 34 | 3 | 17 | 142 | 173 | 310 | 1.25e-47 | 164 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1321080 | AT1G74770.1 | 77.143 | 35 | 8 | 0 | 17 | 51 | 165 | 199 | 1.86e-11 | 60.8 |
| Msa1321080 | AT1G74770.2 | 77.143 | 35 | 8 | 0 | 17 | 51 | 165 | 199 | 2.12e-11 | 60.8 |
Find 25 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTCCTCCAGCAACATTAT+AGG | 0.279336 | 8_4:-26303780 | None:intergenic |
| TGTCTGACCTGAAATTTCTT+TGG | 0.284002 | 8_4:-26304145 | None:intergenic |
| AGGAACTCCAAAGAAATTTC+AGG | 0.336881 | 8_4:+26304138 | Msa1321080:CDS |
| TTGGTCCGAGTTCATTCAAT+AGG | 0.354396 | 8_4:+26304118 | Msa1321080:CDS |
| AGATTGGGTCAACCAGGTTA+AGG | 0.419092 | 8_4:+26304191 | Msa1321080:CDS |
| AGCAACAAACAAACCTTCAC+TGG | 0.431741 | 8_4:+26304033 | Msa1321080:intron |
| TAGTGTTCCTATAATGTTGC+TGG | 0.432236 | 8_4:+26303773 | Msa1321080:CDS |
| TCTTGCAGTGCTGTTTCCAT+TGG | 0.434812 | 8_4:-26303866 | None:intergenic |
| TAACAAAGAAACAGAAGATT+GGG | 0.442902 | 8_4:+26304176 | Msa1321080:CDS |
| CTGCTTGTTTATCTGCTGAC+AGG | 0.474479 | 8_4:-26303814 | None:intergenic |
| TTTCTATATGCAAGAATCCA+TGG | 0.478233 | 8_4:-26304089 | None:intergenic |
| ATAACAAAGAAACAGAAGAT+TGG | 0.489453 | 8_4:+26304175 | Msa1321080:CDS |
| TTTCAGAGTGAGGAAATTCA+AGG | 0.502671 | 8_4:+26304063 | Msa1321080:CDS |
| AACAGAAGATTGGGTCAACC+AGG | 0.542861 | 8_4:+26304185 | Msa1321080:CDS |
| GAGGAAATTCAAGGTTGCCA+TGG | 0.554452 | 8_4:+26304072 | Msa1321080:CDS |
| GAGTTCCTATTGAATGAACT+CGG | 0.571210 | 8_4:-26304123 | None:intergenic |
| TCAAATTTATCCACTGCCGC+AGG | 0.573609 | 8_4:+26303683 | Msa1321080:CDS |
| TTGTTTATCTGCTGACAGGA+AGG | 0.573644 | 8_4:-26303810 | None:intergenic |
| CTGAAAATATGTCCCAGTGA+AGG | 0.605019 | 8_4:-26304046 | None:intergenic |
| AAAGATGGAAAACCTTAACC+TGG | 0.608322 | 8_4:-26304203 | None:intergenic |
| AAGAGCAAGCCTCACTTGTG+TGG | 0.630967 | 8_4:+26303739 | Msa1321080:CDS |
| AATGGAAACAGCACTGCAAG+AGG | 0.632913 | 8_4:+26303868 | Msa1321080:CDS |
| GCAACAAACAAACCTTCACT+GGG | 0.643067 | 8_4:+26304034 | Msa1321080:intron |
| TATGAATTGCCACACAAGTG+AGG | 0.645881 | 8_4:-26303748 | None:intergenic |
| TGTTCCTATAATGTTGCTGG+AGG | 0.651755 | 8_4:+26303776 | Msa1321080:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAGATTTGAATTATTAAAA+AGG | - | chr8_4:26303984-26304003 | None:intergenic | 10.0% |
| ! | GGGGGGGGGGGGGGGGGGGG+GGG | - | chr8_4:26303933-26303952 | None:intergenic | 100.0% |
| ! | GGGGGGGGGGGGGGGGGGGG+GGG | - | chr8_4:26303933-26303952 | None:intergenic | 100.0% |
| ! | GGGGGGGGGGGGGGGGGGGG+GGG | - | chr8_4:26303933-26303952 | None:intergenic | 100.0% |
| !! | TTGCATATAGAAAAATCATT+TGG | + | chr8_4:26304099-26304118 | Msa1321080:CDS | 20.0% |
| !! | TTTTAATGAAATTGCACCAA+TGG | + | chr8_4:26303850-26303869 | Msa1321080:CDS | 25.0% |
| !! | AAATCTAATTGCGAAGATTT+TGG | + | chr8_4:26303996-26304015 | Msa1321080:intron | 25.0% |
| ! | ATAACAAAGAAACAGAAGAT+TGG | + | chr8_4:26304175-26304194 | Msa1321080:CDS | 25.0% |
| ! | TAACAAAGAAACAGAAGATT+GGG | + | chr8_4:26304176-26304195 | Msa1321080:CDS | 25.0% |
| ! | GCTCTTTAGTAGACAATTTT+TGG | - | chr8_4:26303725-26303744 | None:intergenic | 30.0% |
| TTTCTATATGCAAGAATCCA+TGG | - | chr8_4:26304092-26304111 | None:intergenic | 30.0% | |
| !! | GACAATTTTTGGATCAGAAG+AGG | - | chr8_4:26303714-26303733 | None:intergenic | 35.0% |
| TAGTGTTCCTATAATGTTGC+TGG | + | chr8_4:26303773-26303792 | Msa1321080:CDS | 35.0% | |
| !!! | TTGCGAAGATTTTGGTTTCT+TGG | + | chr8_4:26304004-26304023 | Msa1321080:intron | 35.0% |
| !!! | AAGATTTTGGTTTCTTGGCT+TGG | + | chr8_4:26304009-26304028 | Msa1321080:intron | 35.0% |
| TTTCAGAGTGAGGAAATTCA+AGG | + | chr8_4:26304063-26304082 | Msa1321080:CDS | 35.0% | |
| GAGTTCCTATTGAATGAACT+CGG | - | chr8_4:26304126-26304145 | None:intergenic | 35.0% | |
| AGGAACTCCAAAGAAATTTC+AGG | + | chr8_4:26304138-26304157 | Msa1321080:CDS | 35.0% | |
| TGTCTGACCTGAAATTTCTT+TGG | - | chr8_4:26304148-26304167 | None:intergenic | 35.0% | |
| ATCAGAAGAGGAAAAACCTG+CGG | - | chr8_4:26303702-26303721 | None:intergenic | 40.0% | |
| TATGAATTGCCACACAAGTG+AGG | - | chr8_4:26303751-26303770 | None:intergenic | 40.0% | |
| TGTTCCTATAATGTTGCTGG+AGG | + | chr8_4:26303776-26303795 | Msa1321080:CDS | 40.0% | |
| ACTTCCTCCAGCAACATTAT+AGG | - | chr8_4:26303783-26303802 | None:intergenic | 40.0% | |
| TTGTTTATCTGCTGACAGGA+AGG | - | chr8_4:26303813-26303832 | None:intergenic | 40.0% | |
| AGCAACAAACAAACCTTCAC+TGG | + | chr8_4:26304033-26304052 | Msa1321080:intron | 40.0% | |
| GCAACAAACAAACCTTCACT+GGG | + | chr8_4:26304034-26304053 | Msa1321080:intron | 40.0% | |
| CTGAAAATATGTCCCAGTGA+AGG | - | chr8_4:26304049-26304068 | None:intergenic | 40.0% | |
| ! | TGGGACATATTTTCAGAGTG+AGG | + | chr8_4:26304053-26304072 | Msa1321080:CDS | 40.0% |
| TTGGTCCGAGTTCATTCAAT+AGG | + | chr8_4:26304118-26304137 | Msa1321080:CDS | 40.0% | |
| TCAAATTTATCCACTGCCGC+AGG | + | chr8_4:26303683-26303702 | Msa1321080:CDS | 45.0% | |
| CTGCTTGTTTATCTGCTGAC+AGG | - | chr8_4:26303817-26303836 | None:intergenic | 45.0% | |
| !! | TCTTGCAGTGCTGTTTCCAT+TGG | - | chr8_4:26303869-26303888 | None:intergenic | 45.0% |
| AATGGAAACAGCACTGCAAG+AGG | + | chr8_4:26303868-26303887 | Msa1321080:CDS | 45.0% | |
| GAGGAAATTCAAGGTTGCCA+TGG | + | chr8_4:26304072-26304091 | Msa1321080:CDS | 45.0% | |
| AACAGAAGATTGGGTCAACC+AGG | + | chr8_4:26304185-26304204 | Msa1321080:CDS | 45.0% | |
| AGATTGGGTCAACCAGGTTA+AGG | + | chr8_4:26304191-26304210 | Msa1321080:CDS | 45.0% | |
| AAGAGCAAGCCTCACTTGTG+TGG | + | chr8_4:26303739-26303758 | Msa1321080:CDS | 50.0% | |
| TGTGTGTGTGTGTGTGTGTG+GGG | - | chr8_4:26303957-26303976 | None:intergenic | 50.0% | |
| GTGTGTGTGTGTGTGTGTGT+GGG | - | chr8_4:26303958-26303977 | None:intergenic | 50.0% | |
| TGTGTGTGTGTGTGTGTGTG+TGG | - | chr8_4:26303957-26303976 | None:intergenic | 50.0% | |
| AGAGGAAAAACCTGCGGCAG+TGG | - | chr8_4:26303696-26303715 | None:intergenic | 55.0% | |
| TGTGTGTGTGTGTGTGTGGG+GGG | - | chr8_4:26303955-26303974 | None:intergenic | 55.0% | |
| GTGTGTGTGTGTGTGTGTGG+GGG | - | chr8_4:26303956-26303975 | None:intergenic | 55.0% | |
| TGTGTGTGTGTGTGTGGGGG+GGG | - | chr8_4:26303953-26303972 | None:intergenic | 60.0% | |
| GTGTGTGTGTGTGTGTGGGG+GGG | - | chr8_4:26303954-26303973 | None:intergenic | 60.0% | |
| TGTGTGTGTGTGTGGGGGGG+GGG | - | chr8_4:26303951-26303970 | None:intergenic | 65.0% | |
| GTGTGTGTGTGTGTGGGGGG+GGG | - | chr8_4:26303952-26303971 | None:intergenic | 65.0% | |
| TGTGTGTGTGTGGGGGGGGG+GGG | - | chr8_4:26303949-26303968 | None:intergenic | 70.0% | |
| GTGTGTGTGTGTGGGGGGGG+GGG | - | chr8_4:26303950-26303969 | None:intergenic | 70.0% | |
| TGTGTGTGTGGGGGGGGGGG+GGG | - | chr8_4:26303947-26303966 | None:intergenic | 75.0% | |
| GTGTGTGTGTGGGGGGGGGG+GGG | - | chr8_4:26303948-26303967 | None:intergenic | 75.0% | |
| ! | TGTGTGTGGGGGGGGGGGGG+GGG | - | chr8_4:26303945-26303964 | None:intergenic | 80.0% |
| ! | GTGTGTGTGGGGGGGGGGGG+GGG | - | chr8_4:26303946-26303965 | None:intergenic | 80.0% |
| ! | TGTGTGGGGGGGGGGGGGGG+GGG | - | chr8_4:26303943-26303962 | None:intergenic | 85.0% |
| ! | GTGTGTGGGGGGGGGGGGGG+GGG | - | chr8_4:26303944-26303963 | None:intergenic | 85.0% |
| ! | TGTGGGGGGGGGGGGGGGGG+GGG | - | chr8_4:26303941-26303960 | None:intergenic | 90.0% |
| ! | GTGTGGGGGGGGGGGGGGGG+GGG | - | chr8_4:26303942-26303961 | None:intergenic | 90.0% |
| ! | TGGGGGGGGGGGGGGGGGGG+GGG | - | chr8_4:26303939-26303958 | None:intergenic | 95.0% |
| ! | GTGGGGGGGGGGGGGGGGGG+GGG | - | chr8_4:26303940-26303959 | None:intergenic | 95.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 26303654 | 26304224 | 26303654 | ID=Msa1321080;Name=Msa1321080 |
| chr8_4 | mRNA | 26303654 | 26304224 | 26303654 | ID=Msa1321080-mRNA-1;Parent=Msa1321080;Name=Msa1321080-mRNA-1;_AED=0.17;_eAED=0.44;_QI=0|0|0|1|1|1|2|0|142 |
| chr8_4 | exon | 26303654 | 26303889 | 26303654 | ID=Msa1321080-mRNA-1:exon:8990;Parent=Msa1321080-mRNA-1 |
| chr8_4 | exon | 26304035 | 26304224 | 26304035 | ID=Msa1321080-mRNA-1:exon:8991;Parent=Msa1321080-mRNA-1 |
| chr8_4 | CDS | 26303654 | 26303889 | 26303654 | ID=Msa1321080-mRNA-1:cds;Parent=Msa1321080-mRNA-1 |
| chr8_4 | CDS | 26304035 | 26304224 | 26304035 | ID=Msa1321080-mRNA-1:cds;Parent=Msa1321080-mRNA-1 |
| Gene Sequence |
| Protein sequence |
